CN104067971A - Multi-parameter multi-functional tester and method for measuring metabolic level of aquatic animals - Google Patents
Multi-parameter multi-functional tester and method for measuring metabolic level of aquatic animals Download PDFInfo
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Abstract
A multi-parameter multi-functional tester for measuring the metabolic level of aquatic animals comprises a closed container, a water circulation system and an oxygen adjustment system. temperature detection probes, dissolved oxygen detection probes, NH3 detection probes and CO2 detection probes are arranged at the two ends of the closed container, and the probes are connected with a temperature detection control unit, a dissolved oxygen detection control unit, an NH3 detection control unit and a CO2 detection control unit. The water circulation system is arranged between the front end and the rear end of the closed container, and the oxygen adjustment system is connected to a pipeline which is connected with a water pump and a water feeding and draining opening in the front end of the closed container. The multi-parameter multi-functional tester for measuring the metabolic level of the aquatic animals has the advantages that multiple metabolic indexes can be continuously detected and include the oxygen consumption rate, the ammonia excretion rate and carbon dioxide elimination rate of basic metabolism and exercise metabolism, and detection can be carried out under different environment conditions which are set; detection is not limited by time, and indexes of detection is stable and reliable; detection parameters provide basic parameters for technological optimization of released density, transport density, disease diagnosis, nutrition metabolism and cultivation of aquatic products.
Description
Technical field
The invention belongs to technical field of aquaculture, relate in particular to aquatic animal metaboilic level determination techniques.
Background technology
In prior art, aquatic animal metaboilic level is measured and is carried out in a closed container, and tested aquatic animal is put into closed container, within a certain period of time, by measuring the variation of Dissolved Oxygen in Water in closed container, measures tested aquatic animal metabolic function.The deficiencies in the prior art are:
1, can only do static metaboilic level measures;
2, in mensuration process, due to tested aquatic animal in metabolic process by the dissolved oxygen of water body in constantly consuming closed container, thereby the dissolved oxygen of water body in closed container is constantly reduced, the metaboilic level of measuring is not under metastable dissolved oxygen conditions, not the metabolic rule of aquatic animal under normal ecological condition, thereby be not of universal significance;
3, in closed container, water temperature can not regulate, and can not keep the temperature constant of setting;
4, only can measure dissolved oxygen, other metabolic index are not measured.
Summary of the invention
The object of the invention is to develop a kind of can adjusting with temperature, flow velocity and the dissolved oxygen of water body measuring, and can measure analyzer and the assay method of multinomial metabolic index simultaneously, overcome the deficiencies in the prior art.
Technical scheme of the present invention is: the multi-functional aquatic animal metaboilic level of multi-parameter analyzer, comprise closed container, the water body circulatory system and oxygen regulating system, it is characterized in that: described closed container front end is provided with into discharge outlet, rear end is provided with cycle water water inlet, closed container middle part is provided with biological subject and puts into mouth, entering discharge outlet is connected with intake-discharge pipe road, on intake-discharge pipe road, be provided with successively water-inflow and drain valve and heater, biological subject is put into mouth and is provided with seal cover, seal cover is provided with blast pipe, blast pipe is provided with air bleeding valve, a commutator is respectively established at the inner two ends of closed container, closed container both ends are provided with temperature measuring probe, dissolved oxygen detection probe, NH
3detection probe and CO
2detection probe, temperature measuring probe, dissolved oxygen detection probe, NH
3detection probe and CO
2detection probe respectively with temperature detection control module, dissolved oxygen detection control unit, NH
3detection control unit and CO
2detection control unit is connected, the described water body circulatory system is located between closed container rear and front end, the water body circulatory system comprises the water pump being connected by pipeline, circular flow meter and flow control valve, the delivery port of water pump is connected with closed container rear end cycle water water inlet, circular flow meter is located on the pipeline that water pump is connected with closed container rear end cycle water water inlet, on the pipeline that water pump is connected with closed container rear end cycle water water inlet, be also provided with circulation line exhaust outlet, circulation line exhaust outlet is provided with circulation line air bleeding valve, the water inlet of water pump enters discharge outlet with closed container front end and is connected, flow control valve is located at water pump and closed container front end and enters on the pipeline that discharge outlet is connected, described oxygen regulating system comprises oxygen cylinder, oxygen regulating valve and oxygen flow meter, oxygen cylinder gas outlet is connected to water pump and closed container front end by oxygen regulating valve to be entered discharge outlet and is connected before the flow control valve of pipeline, and oxygen flow meter is connected to oxygen cylinder gas outlet and enters discharge outlet with closed container front end and be connected on pipeline.
The multi-functional aquatic animal metaboilic level of multi-parameter of the present invention analyzer, is characterized in that: described commutator is the reticular component equating with closed container internal diameter, and reticular component is that thickness is the dull and stereotyped net of square hole more than 10mm, and square hole dividing plate two ends are streamlined.
The multi-functional aquatic animal metaboilic level of multi-parameter of the present invention analyzer, is characterized in that: described temperature detection control module, dissolved oxygen detection control unit, NH
3detection control unit and CO
2detection control unit is that computer based detects and controller, and built-in computer detects controls software.
The detection method of the multi-functional aquatic animal metaboilic level of multi-parameter of the present invention analyzer, comprises Static Detection, sets under warm condition to detect, set under water volume flow rate condition and detect and set under dissolved oxygen conditions and detect, and it is characterized in that:
Described Static Detection comprises the following steps: open air bleeding valve and circulation line air bleeding valve on seal cover, open water-inflow and drain valve on intake-discharge pipe road, water filling in being provided with into discharge outlet to closed container by closed container front end, until filling with in closed container after water, close on intake-discharge pipe road air bleeding valve and circulation line air bleeding valve on water-inflow and drain valve, seal cover, open closed container biological subject and put into the seal cover of mouth, biological subject is put into closed container, seal cover is closed, in setting-up time, by dissolved oxygen detection probe, NH
3detection probe and CO
2detection probe continuous detecting records dissolved oxygen content, the NH of water body in closed container
3capacity and CO
2capacity, the dissolved oxygen content of surveying, NH
3capacity and CO
2capability value can draw biological subject consumption rate, ammonia N-excretion rates and carbon dioxide elimination metabolic function desired value;
Under described setting temperature condition, detect and set under water volume flow rate condition and detect and comprise the following steps: before water filling, heater being adjusted to design temperature, the injecting process is identical with Static Detection step, in closed container, fill with after water, close water-inflow and drain valve on intake-discharge pipe road, air bleeding valve and circulation line air bleeding valve on seal cover, connect the water body circulatory system, adjust water body circulatory system flow control valve to setting flow, until the temperature of water body in closed container and flow speed stability after setting value, open closed container biological subject and put into the seal cover of mouth, biological subject is put into closed container, seal cover is closed, in setting-up time, by dissolved oxygen detection probe, NH
3detection probe and CO
2detection probe continuous detecting records dissolved oxygen content, the NH of water body in closed container
3capacity and CO
2capacity, the dissolved oxygen content of surveying, NH
3capacity and CO
2capability value can show that biological subject is in consumption rate, ammonia N-excretion rates and the carbon dioxide elimination metabolic function desired value set under warm bar and setting water volume flow rate,
Described setting is set under dissolved oxygen conditions and is detected and comprise the following steps: before water filling, heater is adjusted to design temperature, the injecting process is identical with Static Detection step, in closed container, fill with after water, close water-inflow and drain valve on intake-discharge pipe road, air bleeding valve and circulation line air bleeding valve on seal cover, connect the water body circulatory system, adjust water body circulatory system flow control valve to setting flow, until the temperature of water body in closed container and flow speed stability after setting value, by dissolved oxygen detection probe, measure the dissolved oxygen content of water body in closed container, by oxygen regulating valve adjust to closed container in the suitable gas output of the dissolved oxygen content of water body, the dissolved oxygen content that maintains water body in closed container is stable, open closed container biological subject and put into the seal cover of mouth, biological subject is put into closed container, seal cover is closed, in setting-up time, by dissolved oxygen detection probe, NH
3detection probe and CO
2detection probe continuous detecting records dissolved oxygen content, the NH of water body in closed container
3capacity and CO
2capacity, the NH that surveys
3capacity and CO
2capability value can obtain the metabolic function desired value of setting biological subject consumption rate, ammonia N-excretion rates and carbon dioxide elimination under the warm bar of setting and setting water volume flow rate in dissolved oxygen content water body.
Compared with prior art, the present invention has following beneficial effect:
1, the physiological metabolism index that analyzer of the present invention and detection method can the multinomial biological subjects of continuous detecting, comprises consumption rate, ammonia N-excretion rates and carbon dioxide elimination;
2, can under the environmental condition of different set, detect, obtain the physiological metabolism index of biological subject under varying environment condition;
3, owing to making dissolved oxygen content be not less than the aequum of biological subject to closed container water body delivery of supplemental oxygen, detection can not be subject to time restriction, and the index of detection is more reliable and more stable;
4, both can carry out the detection of basal metabolism, also can carry out the detection of exercise metabolism, for determining that hydrobiological energy metabolism level provides reliable detected parameters;
5, breeding density, traffic density, disease diagnosis, Nutrition and Metabolism and breeding technique optimization that the parameter detecting can be aquaculture product provide underlying parameter.
Accompanying drawing explanation
Fig. 1 is the multi-functional aquatic animal metaboilic level of multi-parameter of the present invention analyzer structural representation
Fig. 2 is the multi-functional aquatic animal metaboilic level of the multi-parameter analyzer structural representation by computer system control
Fig. 3 is commutator structural representation
Fig. 4 is commutator structure cross-sectional schematic
Fig. 5 is carp mean oxygen consumption rate curve map under different temperatures
Fig. 6 is carp ammonia N-excretion rates curve map under different temperatures
Fig. 7 is carp carbon dioxide elimination curve map under different temperatures
In figure, 1, base, 2, temperature measuring probe, 3, water-inflow and drain valve, 4, heater, 5, dissolved oxygen detection probe, 6, commutator, 7, biological subject, 8, biological subject puts into mouth, 9, seal cover exhaust outlet, 9-1, circulation line exhaust outlet, 10, closed container, 11, NH
3detection probe, 12, oxygen cylinder, 13, oxygen flow meter, 14, CO
2detection probe, 15, circular flow meter, 16, flow control valve, 17, water pump, 18, cycle water water inlet, 19, computer based detects and controller.
Embodiment
Below in conjunction with drawings and Examples, the present invention is further described.
The multi-functional aquatic animal metaboilic level of multi-parameter analyzer comprises closed container 10, the water body circulatory system and oxygen regulating system, closed container 10 front ends are provided with into discharge outlet, rear end is provided with cycle water water inlet 18, closed container middle part is provided with biological subject and puts into mouth 8, entering discharge outlet is connected with intake-discharge pipe road, on intake-discharge pipe road, be provided with successively water-inflow and drain valve 3 and heater 4, biological subject is put into mouth 8 and is provided with seal cover, seal cover is provided with seal cover exhaust outlet 9, seal cover exhaust outlet 9 is provided with air bleeding valve, a commutator 6 is respectively established at the inner two ends of closed container, commutator 6 is the reticular components that equate with closed container internal diameter, reticular component is that thickness is the dull and stereotyped net of square hole more than 10mm, square hole dividing plate two ends are streamlined, closed container 10 both ends are provided with temperature measuring probe 2, dissolved oxygen detection probe 5, NH
3detection probe 11 and CO
2detection probe 14, temperature measuring probe 2, dissolved oxygen detection probe 5, NH
3detection probe 11 and CO
2detection probe 14 respectively with temperature detection control module, dissolved oxygen detection control unit, NH
3detection control unit and CO
2detection control unit is connected, temperature detection control module, dissolved oxygen detection control unit, NH
3detection control unit and CO
2detection control unit is that computer based detects and controller 19, and built-in computer detects controls software, the water body circulatory system is located between closed container rear and front end, the water body circulatory system comprises the water pump 17 being connected by pipeline, circular flow meter 15 and flow control valve 16, the delivery port of water pump 17 is connected with closed container rear end cycle water water inlet 18, circular flow meter 15 is located on the pipeline that water pump 17 is connected with closed container rear end cycle water water inlet 18, on the pipeline that water pump 17 is connected with closed container rear end cycle water water inlet 18, be also provided with circulation line exhaust outlet 9-1, circulation line exhaust outlet 9-1 is provided with circulation line air bleeding valve, the water inlet of water pump 17 enters discharge outlet with closed container front end and is connected, flow control valve 16 is located at water pump 17 and closed container front end and enters on the pipeline that discharge outlet is connected, oxygen regulating system comprises oxygen cylinder 12, oxygen regulating valve and oxygen flow meter 13, oxygen cylinder 12 gas outlets are connected to water pump 17 and closed container front end by oxygen regulating valve to be entered discharge outlet and is connected before the flow control valve 16 of pipeline, and oxygen flow meter 13 is connected on oxygen cylinder gas outlet and enters discharge outlet with closed container front end and be connected on pipeline.
Detection method comprises Static Detection, sets under warm condition to detect, set under water volume flow rate condition and detect and set under dissolved oxygen conditions and detect:
Static Detection comprises the following steps: open air bleeding valve and circulation line air bleeding valve on seal cover, open water-inflow and drain valve 3 on intake-discharge pipe road, by closed container front end, enter discharge outlet to the interior water filling of closed container 10, in closed container 10, fill with after water, close on intake-discharge pipe road air bleeding valve and circulation line air bleeding valve on water-inflow and drain valve 3, seal cover, open closed container biological subject and put into the seal cover of mouth 8, biological subject 7 is put into closed container 10, seal cover is closed, in setting-up time, by dissolved oxygen detection probe 5, NH
3detection probe 11 and CO
2detection probe 14 continuous detecting record dissolved oxygen content, the NH of water body in closed container
3capacity and CO
2capacity, the dissolved oxygen content of surveying, NH
3capacity and CO
2capability value can draw biological subject consumption rate, ammonia N-excretion rates and carbon dioxide elimination metabolic function desired value;
Set under warm condition and detect and set under water volume flow rate condition and detect and comprise the following steps: before water filling, heater 4 is adjusted to design temperature, the injecting process is identical with Static Detection step, in closed container 10, fill with after water, close water-inflow and drain valve on intake-discharge pipe road, air bleeding valve and circulation line air bleeding valve on seal cover, connect the water body circulatory system, adjust water body circulatory system flow control valve 16 to setting flow, until the temperature of closed container 10 interior water bodys and flow speed stability after setting value, open closed container biological subject and put into the seal cover of mouth 8, biological subject 7 is put into closed container 10, seal cover is closed, in setting-up time, by dissolved oxygen detection probe 5, NH
3detection probe 11 and CO
2detection probe 14 continuous detecting record dissolved oxygen content, the NH of closed container 10 interior water bodys
3capacity and CO
2capacity, the dissolved oxygen content of surveying, NH
3capacity and CO
2capability value can show that biological subject 7 is in consumption rate, ammonia N-excretion rates and the carbon dioxide elimination metabolic function desired value set under warm bar and setting water volume flow rate,
Described setting is set under dissolved oxygen conditions and is detected and comprise the following steps: before water filling, heater 4 is adjusted to design temperature, the injecting process is identical with Static Detection step, in closed container 10, fill with after water, close water-inflow and drain valve 3 on intake-discharge pipe road, air bleeding valve and circulation line air bleeding valve on seal cover, connect the water body circulatory system, adjust water body circulatory system flow control valve 16 to setting flow, until the temperature of water body in closed container and flow speed stability after setting value, by dissolved oxygen detection probe 5, measure the dissolved oxygen content of closed container 10 interior water bodys, oxygen regulating valve is adjusted to the gas output suitable with the dissolved oxygen content of closed container 10 interior water bodys, the dissolved oxygen content that maintains closed container 10 interior water bodys is stable, open closed container biological subject and put into the seal cover of mouth 8, biological subject 7 is put into airtight appearance 10 devices, seal cover is closed, in setting-up time, by dissolved oxygen detection probe 5, NH
3detection probe 11 and CO
2detection probe 14 continuous detecting record dissolved oxygen content, the NH of closed container 10 interior water bodys
3capacity and CO
2capacity, the NH that surveys
3capacity and CO
2capability value can obtain the metabolic function desired value of setting biological subject consumption rate, ammonia N-excretion rates and carbon dioxide elimination under the warm bar of setting and setting water volume flow rate in dissolved oxygen content water body.
Detection example is to be that the metaboilic level of 0.75kg-1kg/ tail carp adult fish under different temperatures detects to body weight.Detection arranges 7 thermogrades, is respectively 5 ℃, and 10 ℃, 15 ℃, 20 ℃, 25 ℃, 30 ℃, 35 ℃, water salinity is 0.By closed container water inlet, be full of after experimental water, open the seal cover that biological subject is put into mouth 8, put at random 3 carps, seal cover is closed.Adjusting flowmeter flow velocity is 4000L/h, and internal circulating load is 68 times/h, and regulating oxygen regulating system to make the first dissolved oxygen of water body in closed container is 8mg/L.By detection system, record initial dissolved oxygen, ammonia nitrogen and carbon dioxide values with detecting in different period water respectively, be 4 hours detection time.By the calculating to surveyed dissolved oxygen, ammonia nitrogen and carbon dioxide values, draw consumption rate, ammonia excretion rate and carbon dioxide elimination.Computing formula is respectively:
Consumption rate computing formula:
RO
2＝(D
1－D
2)×V/(T×W)
RO in formula
2for consumption rate [mg (kgh)
-1], D
1and D
2be respectively in closed container initial dissolved oxygen content and T content (mgL after the time
-1); V is the volume (L) of water body in closed container; T is detection time (h); W is carp gross weight (g) in closed container:
Ammonia excretion rate computing formula:
Ammonia excretion rate [mg/ (kgh)]=(N
t-N
0) * V/ (T * W)
In formula, N
trepresent the ammonia nitrogen amount of T time, N
0representative detects the ammonia nitrogen amount starting, and V is the volume (L) of water body in closed container; T is detection time (h); W is carp gross weight (g) in closed container:
Carbon dioxide elimination computing formula:
Carbon dioxide elimination CR[mg/ (kgh)]=(C
x-C
0) * V/ (T * W)
Wherein CR is CO
2elimination factor, C
0and C
xbe respectively initial CO in closed container
2content and T content (mgL after the time
-1); V is the volume (L) of water body in closed container; T is detection time (h); W is carp gross weight (g) in closed container.
Fig. 1, Fig. 2 and Fig. 3 curve are that detection computations result: Fig. 1 is carp mean oxygen consumption rate under different temperatures, and Fig. 2 is carp ammonia N-excretion rates under different temperatures, and Fig. 3 is carp carbon dioxide elimination under different temperatures.To Fig. 1, Fig. 2 and Fig. 3 tracing analysis, result is as follows:
Carp consumption rate
From curve, can find out the rising along with temperature, carp consumption rate has obvious variation, shows as the rising along with temperature, and carp consumption rate is seen obvious rising on the whole, and in the time of 5 ℃, carp consumption rate is extremely low, while being only 35 ℃ 21.4% of consumption rate.
Carp ammonia N-excretion rates
Rising along with temperature, the ammonia excretion of carp reduces after taking the lead in raising, in the time of 5 ℃ ammonia N-excretion rates extremely low be 0.02, extremely significantly lower than other each groups (P<0.01), in the time of 10 degrees Celsius to 20 degrees Celsius, it is comparatively slow that ammonia N-excretion rates rises, and during to 25 ℃, ammonia N-excretion rates rises rapidly, during to 30 ℃, reach the highest ammonia N-excretion rates 2.52mg/kgh, and ammonia N-excretion rates starts to decline 35 ℃ time, with 30 ℃ of group significant differences (P<0.05).
Carbon dioxide elimination
Within the scope of 5～30 ℃, along with the carbon dioxide elimination of the rising carp of temperature obviously raises, show as carbon metabolism and strengthen, when carbon dioxide elimination is 5 ℃ in the time of 30 ℃ 21.2 times, while being 10 ℃ 6.6 times.When temperature is during higher than 30 ℃, the carbon dioxide elimination of carp presents obvious downward trend, when carbon dioxide elimination is than 30 ℃ in the time of 35 ℃, reduces 36.6%.The metabolic rate that shows excess Temperature body declines on the contrary.
Claims (4)
1. the multi-functional aquatic animal metaboilic level of multi-parameter analyzer, comprise closed container (10), the water body circulatory system and oxygen regulating system, it is characterized in that: described closed container (10) front end is provided with into discharge outlet, rear end is provided with cycle water water inlet (18), closed container middle part is provided with biological subject and puts into mouthful (8), entering discharge outlet is connected with intake-discharge pipe road, on intake-discharge pipe road, be provided with successively water-inflow and drain valve (3) and heater (4), biological subject is put into mouthful (8) and is provided with seal cover, seal cover is provided with seal cover exhaust outlet (9), seal cover exhaust outlet (9) is provided with air bleeding valve, a commutator (6) is respectively established at the inner two ends of closed container, closed container (10) both ends are provided with temperature measuring probe (2), dissolved oxygen detection probe (5), NH
3detection probe (11) and CO
2detection probe (14), temperature measuring probe (2), dissolved oxygen detection probe (5), NH
3detection probe (11) and CO
2detection probe (14) respectively with temperature detection control module, dissolved oxygen detection control unit, NH
3detection control unit and CO
2detection control unit is connected, the described water body circulatory system is located between closed container rear and front end, the water body circulatory system comprises the water pump (17) being connected by pipeline, circular flow meter (15) and flow control valve (16), the delivery port of water pump (17) is connected with closed container rear end cycle water water inlet (18), circular flow meter (15) is located on the pipeline that water pump (17) is connected with closed container rear end cycle water water inlet (18), on the pipeline that water pump (17) is connected with closed container rear end cycle water water inlet (18), be also provided with circulation line exhaust outlet (9-1), circulation line exhaust outlet (9-1) is provided with circulation line air bleeding valve, the water inlet of water pump (17) enters discharge outlet with closed container front end and is connected, flow control valve (16) is located at water pump (17) and closed container front end and enters on the pipeline that discharge outlet is connected, described oxygen regulating system comprises oxygen cylinder (12), oxygen regulating valve and oxygen flow meter (13), oxygen cylinder (12) gas outlet is connected to water pump (17) and closed container front end by oxygen regulating valve, and to enter the be connected flow control valve (16) of pipeline of discharge outlet front, and oxygen flow meter (13) is connected to oxygen cylinder gas outlet and enters discharge outlet with closed container front end and be connected on pipeline.
2. the multi-functional aquatic animal metaboilic level of multi-parameter analyzer according to claim 1, it is characterized in that: described commutator (6) is the reticular component equating with closed container internal diameter, reticular component is that thickness is the dull and stereotyped net of square hole more than 10mm, and square hole dividing plate two ends are streamlined.
3. the multi-functional aquatic animal metaboilic level of multi-parameter analyzer according to claim 1, is characterized in that: described temperature detection control module, dissolved oxygen detection control unit, NH
3detection control unit and CO
2detection control unit is that computer based detects and controller (19), and built-in computer detects controls software.
4. the detection method of the multi-functional aquatic animal metaboilic level of multi-parameter analyzer described in claim 1, comprises under Static Detection, design temperature condition detecting, setting under water volume flow rate condition and detects and set under dissolved oxygen conditions and detect, and it is characterized in that:
Described Static Detection comprises the following steps: open air bleeding valve and circulation line air bleeding valve on seal cover, open water-inflow and drain valve on intake-discharge pipe road (3), by closed container front end, enter discharge outlet to the interior water filling of closed container (10), in closed container (10), fill with after water, close water-inflow and drain valve on intake-discharge pipe road (3), air bleeding valve and circulation line air bleeding valve on seal cover, open closed container biological subject and put into the seal cover of mouthful (8), biological subject (7) is put into closed container (10), seal cover is closed, after biological subject peace and quiet are stable, in setting-up time, by dissolved oxygen detection probe (5), NH
3detection probe (11) and CO
2detection probe (14) continuous detecting records dissolved oxygen content, the NH of water body in closed container
3-N content and CO
2content, dissolved oxygen content, the NH of the different time of surveying
3-N content and CO
2content value is calculated the metabolic function desired value that can draw biological subject consumption rate, ammonia N-excretion rates and carbon dioxide elimination by difference,
Under described design temperature condition, detect and set under water volume flow rate condition and detect and comprise the following steps: before water filling, heater (4) being adjusted to design temperature, the injecting process is identical with Static Detection step, closed container is filled with after water in (10), close water-inflow and drain valve on intake-discharge pipe road, air bleeding valve and circulation line air bleeding valve on seal cover, connect the water body circulatory system, adjust water body circulatory system flow control valve (16) to setting flow, in closed container (10), the temperature of water body and flow speed stability are after setting value, open closed container biological subject and put into the seal cover of mouthful (8), biological subject (7) is put into closed container (10), seal cover is closed, in setting-up time, by dissolved oxygen detection probe (5), NH
3detection probe (11) and CO
2detection probe (14) continuous detecting records dissolved oxygen content, the NH of the interior water body of closed container (10)
3-N content and CO
2content, dissolved oxygen content, the NH of the different time of surveying
3-N content and CO
2content value is calculated the metabolic function desired value that can draw biological subject consumption rate, ammonia N-excretion rates and carbon dioxide elimination by difference,
Detection under described setting dissolved oxygen conditions comprises the following steps: before water filling, heater (4) is adjusted to design temperature, the injecting process is identical with Static Detection step, closed container is filled with after water in (10), close water-inflow and drain valve on intake-discharge pipe road (3), air bleeding valve and circulation line air bleeding valve on seal cover, connect the water body circulatory system, adjust water body circulatory system flow control valve (16) to setting flow, until the temperature of water body in closed container and flow speed stability after setting value, by dissolved oxygen detection probe (5), detect the dissolved oxygen content of the interior water body of closed container (10), oxygen regulating valve is adjusted to the gas output suitable with the dissolved oxygen content of the interior water body of closed container (10), the dissolved oxygen content that maintains the interior water body of closed container (10) is stable, open closed container biological subject and put into the seal cover of mouthful (8), biological subject (7) is put into airtight appearance (10) device, seal cover is closed, in setting-up time, by dissolved oxygen detection probe (5), NH
3detection probe (11) and CO
2detection probe (14) continuous detecting records dissolved oxygen content, the NH of the interior water body of closed container (10)
3-N content and CO
2content, dissolved oxygen content, the NH of the different time of surveying
3-N content and CO
2content value is calculated and can be obtained the metabolic function desired value of setting biological subject consumption rate, ammonia N-excretion rates and carbon dioxide elimination under the warm bar of setting and setting water volume flow rate in dissolved oxygen content water body by difference.
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| CN108354622A (en) * | 2018-02-09 | 2018-08-03 | 华中科技大学同济医学院附属协和医院 | A kind of aquatile detection device and system reflecting tomoscan based on positive electron |
| CN109349203A (en) * | 2018-09-19 | 2019-02-19 | 河海大学 | A kind of accurate automatic control system of aquaculture pool dissolved oxygen and its control method |
| CN110063278A (en) * | 2019-05-28 | 2019-07-30 | 中国水产科学研究院渔业机械仪器研究所 | The device and its working method of fish consumption rate are tested under controllable temperature and flow velocity |
| CN112903939A (en) * | 2021-01-20 | 2021-06-04 | 杨华明 | Mobile intelligent multi-chamber united-row respiratory metabolism measuring device |
| WO2023244578A1 (en) * | 2022-06-13 | 2023-12-21 | Dohner Stephanie Marie | Devices, methods, and systems for collecting aquatic field metabolic rate data |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5681536A (en) * | 1996-05-07 | 1997-10-28 | Nebraska Public Power District | Injection lance for uniformly injecting anhydrous ammonia and air into a boiler cavity |
| JP2009082124A (en) * | 2008-01-08 | 2009-04-23 | Takahiro Yamamoto | Fish observation cistern |
| CN201936200U (en) * | 2010-12-20 | 2011-08-17 | 山东海益宝水产股份有限公司 | Regulation and control system for dissolved oxygen in culturing water body |
| CN103181345A (en) * | 2012-09-25 | 2013-07-03 | 杭州千岛湖鲟龙科技股份有限公司 | Liquid-oxygen oxygen-increasing high-density sturgeon culturing method |
| CN103202254A (en) * | 2013-04-16 | 2013-07-17 | 中国水产科学研究院长江水产研究所 | Fish swimming capability measuring method and submerged open-type testing device |
| CN203942943U (en) * | 2014-07-16 | 2014-11-19 | 大连海洋大学 | The multi-functional aquatic animal metaboilic level of multi-parameter analyzer |
-
2014
- 2014-07-16 CN CN201410341505.4A patent/CN104067971B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5681536A (en) * | 1996-05-07 | 1997-10-28 | Nebraska Public Power District | Injection lance for uniformly injecting anhydrous ammonia and air into a boiler cavity |
| JP2009082124A (en) * | 2008-01-08 | 2009-04-23 | Takahiro Yamamoto | Fish observation cistern |
| CN201936200U (en) * | 2010-12-20 | 2011-08-17 | 山东海益宝水产股份有限公司 | Regulation and control system for dissolved oxygen in culturing water body |
| CN103181345A (en) * | 2012-09-25 | 2013-07-03 | 杭州千岛湖鲟龙科技股份有限公司 | Liquid-oxygen oxygen-increasing high-density sturgeon culturing method |
| CN103202254A (en) * | 2013-04-16 | 2013-07-17 | 中国水产科学研究院长江水产研究所 | Fish swimming capability measuring method and submerged open-type testing device |
| CN203942943U (en) * | 2014-07-16 | 2014-11-19 | 大连海洋大学 | The multi-functional aquatic animal metaboilic level of multi-parameter analyzer |
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| CN108354622A (en) * | 2018-02-09 | 2018-08-03 | 华中科技大学同济医学院附属协和医院 | A kind of aquatile detection device and system reflecting tomoscan based on positive electron |
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| CN109349203A (en) * | 2018-09-19 | 2019-02-19 | 河海大学 | A kind of accurate automatic control system of aquaculture pool dissolved oxygen and its control method |
| CN110063278A (en) * | 2019-05-28 | 2019-07-30 | 中国水产科学研究院渔业机械仪器研究所 | The device and its working method of fish consumption rate are tested under controllable temperature and flow velocity |
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