CN104066838A - 变体α-淀粉酶及其使用方法 - Google Patents

变体α-淀粉酶及其使用方法 Download PDF

Info

Publication number
CN104066838A
CN104066838A CN201280063219.5A CN201280063219A CN104066838A CN 104066838 A CN104066838 A CN 104066838A CN 201280063219 A CN201280063219 A CN 201280063219A CN 104066838 A CN104066838 A CN 104066838A
Authority
CN
China
Prior art keywords
amylase
variant
amino acid
composition
detergent
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201280063219.5A
Other languages
English (en)
Chinese (zh)
Inventor
L·G·卡斯康-佩雷拉
M·科尔克曼
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Danisco US Inc
Original Assignee
Danisco US Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Danisco US Inc filed Critical Danisco US Inc
Publication of CN104066838A publication Critical patent/CN104066838A/zh
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/386—Preparations containing enzymes, e.g. protease or amylase
    • C11D3/38618—Protease or amylase in liquid compositions only
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2414—Alpha-amylase (3.2.1.1.)
    • C12N9/2417—Alpha-amylase (3.2.1.1.) from microbiological source
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/386—Preparations containing enzymes, e.g. protease or amylase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01001—Alpha-amylase (3.2.1.1)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Detergent Compositions (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
CN201280063219.5A 2011-12-22 2012-12-18 变体α-淀粉酶及其使用方法 Pending CN104066838A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201161579356P 2011-12-22 2011-12-22
US61/579,356 2011-12-22
PCT/US2012/070334 WO2013096305A1 (en) 2011-12-22 2012-12-18 Variant alpha-amylases and methods of use, thereof

Publications (1)

Publication Number Publication Date
CN104066838A true CN104066838A (zh) 2014-09-24

Family

ID=47436284

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201280063219.5A Pending CN104066838A (zh) 2011-12-22 2012-12-18 变体α-淀粉酶及其使用方法

Country Status (7)

Country Link
US (1) US20140342431A1 (en:Method)
EP (1) EP2794867A1 (en:Method)
CN (1) CN104066838A (en:Method)
BR (1) BR112014015421A2 (en:Method)
CA (1) CA2858252A1 (en:Method)
IN (1) IN2014DN03298A (en:Method)
WO (1) WO2013096305A1 (en:Method)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104379737B (zh) 2012-06-08 2018-10-23 丹尼斯科美国公司 对淀粉聚合物具有增强的活性的变体α淀粉酶
WO2016201069A1 (en) 2015-06-09 2016-12-15 Danisco Us Inc Low-density enzyme-containing particles
WO2024137248A1 (en) 2022-12-19 2024-06-27 Novozymes A/S Compositions comprising arabinofuranosidases and a xylanase, and use thereof for increasing hemicellulosic fiber solubilization
WO2024137246A1 (en) 2022-12-19 2024-06-27 Novozymes A/S Carbohydrate esterase family 1 (ce1) polypeptides having ferulic acid esterase and/or acetyl xylan esterase activity and polynucleotides encoding same
CN120380158A (zh) 2022-12-19 2025-07-25 诺维信公司 用于降低发酵产物生产方法后端的浆体粘度的方法
WO2024137250A1 (en) 2022-12-19 2024-06-27 Novozymes A/S Carbohydrate esterase family 3 (ce3) polypeptides having acetyl xylan esterase activity and polynucleotides encoding same
CN115960870A (zh) * 2022-12-22 2023-04-14 福州三合元生物科技有限公司 提高淀粉酶热稳定性的方法
WO2025128568A1 (en) 2023-12-11 2025-06-19 Novozymes A/S Composition and use thereof for increasing hemicellulosic fiber solubilization
CN118222546B (zh) * 2024-05-27 2024-07-19 潍坊康地恩生物科技有限公司 一种淀粉酶突变体及其应用

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2009098229A2 (en) * 2008-02-04 2009-08-13 Danisco Us Inc., Genencor Division A method of preparing food products using ts23 alpha-amylase
CN101848985A (zh) * 2007-11-05 2010-09-29 丹尼斯科美国公司 具有改变性质的杆菌物种TS-23的α-淀粉酶变体
WO2010115021A2 (en) * 2009-04-01 2010-10-07 Danisco Us Inc. Compositions and methods comprising alpha-amylase variants with altered properties

Family Cites Families (68)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB1372034A (en) 1970-12-31 1974-10-30 Unilever Ltd Detergent compositions
GB1483591A (en) 1973-07-23 1977-08-24 Novo Industri As Process for coating water soluble or water dispersible particles by means of the fluid bed technique
GB1590432A (en) 1976-07-07 1981-06-03 Novo Industri As Process for the production of an enzyme granulate and the enzyme granuate thus produced
DK187280A (da) 1980-04-30 1981-10-31 Novo Industri As Ruhedsreducerende middel til et fuldvaskemiddel fuldvaskemiddel og fuldvaskemetode
DK263584D0 (da) 1984-05-29 1984-05-29 Novo Industri As Enzymholdige granulater anvendt som detergentadditiver
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
DK171161B1 (da) 1985-03-28 1996-07-08 Hoffmann La Roche Fremgangsmåde til påvisning af forekomst eller fravær af mindst én specifik nukleinsyresekvens i en prøve eller til skelnen mellem to forskellige nukleinsyresekvenser i denne prøve
US4933287A (en) 1985-08-09 1990-06-12 Gist-Brocades N.V. Novel lipolytic enzymes and their use in detergent compositions
EG18543A (en) 1986-02-20 1993-07-30 Albright & Wilson Protected enzyme systems
DK122686D0 (da) 1986-03-17 1986-03-17 Novo Industri As Fremstilling af proteiner
ATE110768T1 (de) 1986-08-29 1994-09-15 Novo Nordisk As Enzymhaltiger reinigungsmittelzusatz.
NZ221627A (en) 1986-09-09 1993-04-28 Genencor Inc Preparation of enzymes, modifications, catalytic triads to alter ratios or transesterification/hydrolysis ratios
ATE125865T1 (de) 1987-08-28 1995-08-15 Novo Nordisk As Rekombinante humicola-lipase und verfahren zur herstellung von rekombinanten humicola-lipasen.
JPS6474992A (en) 1987-09-16 1989-03-20 Fuji Oil Co Ltd Dna sequence, plasmid and production of lipase
ATE129523T1 (de) 1988-01-07 1995-11-15 Novo Nordisk As Spezifische protease.
DK6488D0 (da) 1988-01-07 1988-01-07 Novo Industri As Enzymer
JP3079276B2 (ja) 1988-02-28 2000-08-21 天野製薬株式会社 組換え体ｄｎａ、それを含むシュードモナス属菌及びそれを用いたリパーゼの製造法
JP2728531B2 (ja) 1988-03-24 1998-03-18 ノボ ノルディスク アクティーゼルスカブ セルラーゼ調製品
US5776757A (en) 1988-03-24 1998-07-07 Novo Nordisk A/S Fungal cellulase composition containing alkaline CMC-endoglucanase and essentially no cellobiohydrolase and method of making thereof
GB8915658D0 (en) 1989-07-07 1989-08-23 Unilever Plc Enzymes,their production and use
US5427936A (en) 1990-04-14 1995-06-27 Kali-Chemie Aktiengesellschaft Alkaline bacillus lipases, coding DNA sequences therefor and bacilli, which produce these lipases
BR9106435A (pt) 1990-05-09 1993-05-04 Novo Nordisk As Preparado de celulase,enzima demonstrando atividade de andoglucanase,enzima de endoglucanase,construcao de dna,vetor de expressao celula,processo para produzir uma enzima de endoglucanase,aditivo composicao detergente,e processo para reduzir a taxa em que os tecidos contendo celulose,se tornam asperos,prover clareamento da cor de tecidos contendo celulose colorida,prover uma variacao localizada da cor de tecidos contendo colorida,e melhorar as propriedades de drenagem de polpa
DK115890D0 (da) 1990-05-09 1990-05-09 Novo Nordisk As Enzym
WO1992005249A1 (en) 1990-09-13 1992-04-02 Novo Nordisk A/S Lipase variants
DE69126879T2 (de) 1990-09-28 1998-02-19 The Procter & Gamble Co., Cincinnati, Ohio Polyhydroxyfettsäureamidtenside zur erhöhung der enzymleistung
ATE219136T1 (de) 1991-01-16 2002-06-15 Procter & Gamble Kompakte waschmittelzusammensetzungen mit hochaktiven cellulasen
PL170474B1 (pl) 1991-04-30 1996-12-31 Procter & Gamble C iekla kom pozycja detergentow a PL PL PL
EP0511456A1 (en) 1991-04-30 1992-11-04 The Procter & Gamble Company Liquid detergents with aromatic borate ester to inhibit proteolytic enzyme
ATE168130T1 (de) 1991-05-01 1998-07-15 Novo Nordisk As Stabilisierte enzyme und waschmittelzusammensetzungen
DK72992D0 (da) 1992-06-01 1992-06-01 Novo Nordisk As Enzym
DK88892D0 (da) 1992-07-06 1992-07-06 Novo Nordisk As Forbindelse
JP3681750B2 (ja) 1992-10-06 2005-08-10 ノボザイムス アクティーゼルスカブ セルラーゼ変異体
US5281526A (en) 1992-10-20 1994-01-25 Solvay Enzymes, Inc. Method of purification of amylase by precipitation with a metal halide and 4-hydroxybenzic acid or a derivative thereof
DE69434242T2 (de) 1993-04-27 2006-01-12 Genencor International, Inc., Palo Alto Neuartige Lipasevarianten zur Verwendung in Reinigungsmitteln
DK52393D0 (en:Method) 1993-05-05 1993-05-05 Novo Nordisk As
JP2859520B2 (ja) 1993-08-30 1999-02-17 ノボ ノルディスク アクティーゼルスカブ リパーゼ及びそれを生産する微生物及びリパーゼ製造方法及びリパーゼ含有洗剤組成物
JPH09503664A (ja) 1993-10-13 1997-04-15 ノボ ノルディスク アクティーゼルスカブ Ｈ▲下２▼ｏ▲下２▼安定ペルオキシダーゼ変異体
JPH07143883A (ja) 1993-11-24 1995-06-06 Showa Denko Kk リパーゼ遺伝子及び変異体リパーゼ
JP3553958B2 (ja) 1994-02-22 2004-08-11 ノボザイムス アクティーゼルスカブ 脂質分解酵素の変異体の製造方法
EP1632557B1 (en) 1994-03-08 2011-02-23 Novozymes A/S Novel alkaline cellulases
AU2524695A (en) 1994-05-04 1995-11-29 Genencor International, Inc. Lipases with improved surfactant resistance
WO1995035381A1 (en) 1994-06-20 1995-12-28 Unilever N.V. Modified pseudomonas lipases and their use
WO1996000292A1 (en) 1994-06-23 1996-01-04 Unilever N.V. Modified pseudomonas lipases and their use
JPH10507073A (ja) 1994-10-06 1998-07-14 ノボ ノルディスク アクティーゼルスカブ エンドグルカナーゼ活性を有する酵素及び酵素調製品
BE1008998A3 (fr) 1994-10-14 1996-10-01 Solvay Lipase, microorganisme la produisant, procede de preparation de cette lipase et utilisations de celle-ci.
US5827719A (en) 1994-10-26 1998-10-27 Novo Nordisk A/S Enzyme with lipolytic activity
JPH08228778A (ja) 1995-02-27 1996-09-10 Showa Denko Kk 新規なリパーゼ遺伝子及びそれを用いたリパーゼの製造方法
DE815209T1 (de) 1995-03-17 1998-06-25 Novonordisk As Neue endoglukanase
EP0839186B1 (en) 1995-07-14 2004-11-10 Novozymes A/S A modified enzyme with lipolytic activity
JP4068142B2 (ja) 1995-08-11 2008-03-26 ノボザイムス アクティーゼルスカブ 新規の脂肪分解酵素
AU3938997A (en) 1996-08-26 1998-03-19 Novo Nordisk A/S A novel endoglucanase
WO1998012307A1 (en) 1996-09-17 1998-03-26 Novo Nordisk A/S Cellulase variants
DE69718351T2 (de) 1996-10-08 2003-11-20 Novozymes A/S, Bagsvaerd Diaminobenzoesäure derivate als farbstoffvorläufer
US6300116B1 (en) 1996-11-04 2001-10-09 Novozymes A/S Modified protease having improved autoproteolytic stability
AU4772697A (en) 1996-11-04 1998-05-29 Novo Nordisk A/S Subtilase variants and compositions
WO1998034946A1 (en) 1997-02-12 1998-08-13 Massachusetts Institute Of Technology Daxx, a novel fas-binding protein that activates jnk and apoptosis
DE19736591A1 (de) 1997-08-22 1999-02-25 Peter Prof Dr Hegemann Verfahren zum Herstellen von Nukleinsäurepolymeren
US6254645B1 (en) 1999-08-20 2001-07-03 Genencor International, Inc. Enzymatic modification of the surface of a polyester fiber or article
US6933140B1 (en) 1999-11-05 2005-08-23 Genencor International, Inc. Enzymes useful for changing the properties of polyester
WO2002014490A2 (en) 2000-08-11 2002-02-21 Genencor International, Inc. Bacillus transformation, transformants and mutant libraries
DE10260805A1 (de) 2002-12-23 2004-07-22 Geneart Gmbh Verfahren und Vorrichtung zum Optimieren einer Nucleotidsequenz zur Expression eines Proteins
PT1689859E (pt) 2003-12-03 2011-06-01 Danisco Us Inc Per-hidrolase
US7754460B2 (en) 2003-12-03 2010-07-13 Danisco Us Inc. Enzyme for the production of long chain peracid
US8703459B2 (en) 2003-12-05 2014-04-22 The United States Of America, As Represented By The Secretary, Department Of Health & Human Services Catalytic domains of beta(1,4)-galactosyltransferase I having altered metal ion specificity
US7723083B2 (en) 2005-12-13 2010-05-25 E.I. Du Pont De Nemours And Company Production of peracids using an enzyme having perhydrolysis activity
US7951566B2 (en) 2005-12-13 2011-05-31 E.I. Du Pont De Nemours And Company Production of peracids using an enzyme having perhydrolysis activity
CN102239255B (zh) 2008-10-03 2015-11-25 纳幕尔杜邦公司 改善过水解酶用于酶促过酸生成

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101848985A (zh) * 2007-11-05 2010-09-29 丹尼斯科美国公司 具有改变性质的杆菌物种TS-23的α-淀粉酶变体
WO2009098229A2 (en) * 2008-02-04 2009-08-13 Danisco Us Inc., Genencor Division A method of preparing food products using ts23 alpha-amylase
WO2010115021A2 (en) * 2009-04-01 2010-10-07 Danisco Us Inc. Compositions and methods comprising alpha-amylase variants with altered properties

Also Published As

Publication number Publication date
CA2858252A1 (en) 2013-06-27
US20140342431A1 (en) 2014-11-20
IN2014DN03298A (en:Method) 2015-06-26
BR112014015421A2 (pt) 2019-09-24
EP2794867A1 (en) 2014-10-29
WO2013096305A1 (en) 2013-06-27

Similar Documents

Publication Publication Date Title
US8877479B2 (en) Halomonas strain WDG195-related alpha-amylases, and methods of use, thereof
EP2406373B1 (en) Bacillus megaterium strain dsm90-related alpha-amylases, and methods of use, thereof
US20200087644A1 (en) Alpha-amylase combinatorial variants
US8470758B2 (en) Detergent compositions and methods of use for an alpha-amylase polypeptide of bacillus species 195
CN104066838A (zh) 变体α-淀粉酶及其使用方法
CN104379738A (zh) 衍生自噬纤维菌属物种(CYTOPHAGA SP.)淀粉酶|(CSPAMY2)的α淀粉酶的α淀粉酶变体
CN105960456A (zh) 对蛋白酶裂解敏感性降低的变体α-淀粉酶及其使用方法
JP2012522875A (ja) アルファ‐アミラーゼ及びプロテアーゼを含む洗浄組成物及び方法
WO2013063460A2 (en) Variant maltohexaose-forming alpha-amylase variants
US8815559B2 (en) Amylase from nesterenkonia and methods of use, thereof
HK1166819A (en) Bacillus megaterium strain dsm90-related alpha-amylases, and methods of use, thereof

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
C02 Deemed withdrawal of patent application after publication (patent law 2001)
WD01 Invention patent application deemed withdrawn after publication

Application publication date: 20140924