CN104054565B - A kind of hardening matrix improving bletilla striata plantlet in vitro survival rate - Google Patents
A kind of hardening matrix improving bletilla striata plantlet in vitro survival rate Download PDFInfo
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- CN104054565B CN104054565B CN201410263929.3A CN201410263929A CN104054565B CN 104054565 B CN104054565 B CN 104054565B CN 201410263929 A CN201410263929 A CN 201410263929A CN 104054565 B CN104054565 B CN 104054565B
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Abstract
A kind of hardening matrix improving bletilla striata plantlet in vitro survival rate provided by the invention, comprise peat soil and vitamin B compound, the weight of described vitamin B compound is the 0.02-0.05% of peat soil weight.These bletilla striata training tissue culture seedling base starting material wide material sources, with low cost, greatly can improve the survival rate of bletilla striata plantlet in vitro.
Description
Technical field
The invention belongs to agricultural cultivation field, particularly a kind of hardening matrix improving bletilla striata plantlet in vitro survival rate.
Background technology
The bletilla striata, is perennial herb bulbous plant (block root), plant height 18-60 centimetre.Pseudobulb oblate spheroid, endless belt like upper mask water chestnut, rich viscosity.Stem is sturdy, rigidness.Leaf 4-6 piece, long and narrow circle or lanceolar, long 8-29 centimetre, wide 1.5-4 centimetre, tip is gradually sharp, and base portion is received narrow one-tenth sheath and embraced stem.Inflorescence tool 3-10 flower, often not branch or extremely seldom branch; Rhachis is more or less tortuous in " it " shape; Petal length of a film round shape lanceolar, long 2-2.5 centimetre, blooms and often withers and falls; Hua great, aubergine or pink; Sepal and petal are closely isometric, long and narrow circle, long 25-30 millimeter, wide 6-8 millimeter, the anxious point of tip; Petal is slightly wide compared with sepal; Lip compared with sepal and petal slightly short, the shape of falling ovum is oval, long 23-28 millimeter, white band aubergine, tool purple arteries and veins; On lip dish, mask 5 typhloscle sheets, extend the nearly top of middle sliver from base portion, are only wavy on middle sliver; Stamen column length 18-20 millimeter, column, the narrow wing of tool, slightly bow.The florescence 4-month.[2] have mutation to spend the bletilla striata in vain, floral white, garden-variety still has indigo plant, Huang, pink isochrome.
The cultivation of the bletilla striata is divided into several stage:
Breeding: conventional division propagation.Spring young leaves sprout before or autumn and winter overground part withered after the bletilla striata bletilla striata, the old strain of turn up, segmentation pseudobulb carries out point planting, and every strain can divide 3-5 strain, and every strain must be with terminal bud.Also can adopt seed propagation, but because of seed tiny, hypoplasia, need use medium aseptic inoculation, complicated operation, therefore seldom used.
Hardening: the plantlet in vitro of the bletilla striata, after the taking-up of laboratory, needs the hardening (domestication) through 2-4 month could transplant training and goes in ground.
Cultivation: plowed soils before ground plants, uses sufficient base manure.Plant at the beginning of 3 months, the depth of planting 3 ㎝.Vegetative period need keep ground moistening, notices that weeding is loosened the soil, fertilising in every 2 weeks 1 time.General cultivation is bloomed for latter 2 months.Hua Houzhi executes 1 phosphate fertilizer mid-August, and block root growth can be made to enrich.
Bletilla striata division propagation efficiency is low, of poor benefits.Through research, the synthetic medium aseptic seeding technology of the bletilla striata is quite ripe, and germination rate is higher.But in hardening link, because matrix selection is improper or component ratio is not right, after bletilla striata plantlet in vitro bottle outlet, growth conditions is bad, and survival rate is lower, which greatly limits and utilizes tissue culture technique large-scale production bletilla striata seedling.Therefore, the research for bletilla striata plantlet in vitro domestication growth period condition is extremely urgent.
Summary of the invention
Goal of the invention: in order to overcome above-mentioned the deficiencies in the prior art, the object of the present invention is to provide a kind of hardening matrix that can improve bletilla striata plantlet in vitro survival rate.
Technical scheme: a kind of hardening matrix improving bletilla striata plantlet in vitro survival rate provided by the invention, comprise peat soil and vitamin B compound, the weight of described vitamin B compound is the 0.02-0.05% of peat soil weight.
Preferably, the hardening matrix of described raising bletilla striata plantlet in vitro survival rate, comprise peat soil and vitamin B compound, the weight of described vitamin B compound is the 0.03-0.04% of peat soil weight.
More preferably, described vitamin B compound comprises the combination of one or more in vitamin B1, vitamin B2, vitamin B6, cobalamin, nicotinic acid, pantothenic acid and folic acid.
More preferably, every 100 parts of vitamin B compounds comprise cobalamin 0-30 part, vitamin B2 10-20 part, vitamin B6 10-20 part, cobalamin 5-10 part, nicotinic acid 10-20 part, pantothenic acid 5-10 part and folic acid 10-20 part.
Beneficial effect: bletilla striata training tissue culture seedling base starting material wide material sources provided by the invention, with low cost, can improve the survival rate of bletilla striata plantlet in vitro greatly.
Embodiment
Embodiment 1
Improve the hardening matrix of bletilla striata plantlet in vitro survival rate, comprise peat soil and vitamin B compound, the weight of described vitamin B compound is 0.03% of peat soil weight.
Wherein, every 100 parts of vitamin B compounds comprise cobalamin 5 parts, vitamin B2 15 parts, vitamin B6 15 parts, cobalamin 8 parts, 15 parts, nicotinic acid, 7 parts, pantothenic acid and 15 parts, folic acid.
Embodiment 2
Improve the hardening matrix of bletilla striata plantlet in vitro survival rate, comprise peat soil and vitamin B compound, the weight of described vitamin B compound is 0.04% of peat soil weight.
Wherein, every 100 parts of vitamin B compounds comprise orotic acid 0 part, vitamin B2 10 parts, vitamin B6 20 parts, cobalamin 10 parts, 10 parts, nicotinic acid, 10 parts, pantothenic acid and 10 parts, folic acid.
Embodiment 3
Improve the hardening matrix of bletilla striata plantlet in vitro survival rate, comprise peat soil and vitamin B compound, the weight of described vitamin B compound is 0.05% of peat soil weight.
Wherein, every 100 parts of vitamin B compounds comprise cobalamin 0 part, vitamin B2 20 parts, vitamin B6 10 parts, cobalamin 5 parts, 20 parts, nicotinic acid, 5 parts, pantothenic acid and 20 parts, folic acid.
Embodiment 4
Improve the hardening matrix of bletilla striata plantlet in vitro survival rate, comprise peat soil and vitamin B compound, the weight of described vitamin B compound is 0.02% of peat soil weight.
Wherein, every 100 parts of vitamin B compounds comprise cobalamin 5 parts, vitamin B2 15 parts, vitamin B6 15 parts, cobalamin 8 parts, 15 parts, nicotinic acid, 7 parts, pantothenic acid and 15 parts, folic acid.
Embodiment 5
Utilize the hardening matrix hardening of the raising bletilla striata plantlet in vitro survival rate of embodiment 1 to 4, arrange control group (peat soil), result is as follows simultaneously.
Table 1 improves the survival rate of the hardening matrix hardening of bletilla striata plantlet in vitro survival rate
| Matrix | Hardening number/strain | Cultivation survives number/strain | Survival rate/% |
| Embodiment 1 | 100 | 95 | 95 |
| Embodiment 2 | 100 | 92 | 92 |
| Embodiment 3 | 100 | 91 | 91 |
| Embodiment 4 | 100 | 94 | 94 |
| Control group | 100 | 71 | 71 |
Claims (2)
1. improve a hardening matrix for bletilla striata plantlet in vitro survival rate, it is characterized in that: comprise peat soil and vitamin B compound, the weight of described vitamin B compound is the 0.02-0.05% of peat soil weight; Every 100 parts of vitamin B compounds comprise cobalamin 0-30 part, vitamin B2 10-20 part, vitamin B6 10-20 part, cobalamin 5-10 part, nicotinic acid 10-20 part, pantothenic acid 5-10 part and folic acid 10-20 part.
2. a kind of hardening matrix improving bletilla striata plantlet in vitro survival rate according to claim 1, it is characterized in that: comprise peat soil and vitamin B compound, the weight of described vitamin B compound is the 0.03-0.04% of peat soil weight.
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| CN104054565B true CN104054565B (en) | 2016-04-20 |
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Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105123328B (en) * | 2015-07-31 | 2017-08-25 | 河南科技大学 | A kind of tuber of pinellia training tissue culture seedling, domestication matrix and its preparation and application |
| CN105453942A (en) * | 2015-12-09 | 2016-04-06 | 遵义市黔北绿野科技产业有限责任公司 | Method for increasing survival rate of hardening seedlings of rhizoma bletillae tissue culture seedlings |
| CN106577002A (en) * | 2016-12-23 | 2017-04-26 | 江苏农林职业技术学院 | Bletilla striata culture medium, and preparation method and application thereof |
| CN107637473A (en) * | 2017-10-30 | 2018-01-30 | 南京工业大学大丰海洋产业研究院 | A kind of iris training tissue culture seedling matrix |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102283115A (en) * | 2011-06-28 | 2011-12-21 | 陕西科技大学 | Method for quickly reproducing bletilla striata seedlings |
| CN102972290A (en) * | 2012-11-15 | 2013-03-20 | 贵州师范大学 | Method for improving transplanting survival rate of rhizoma bletillae tissue culture seedling |
| CN103583365A (en) * | 2013-11-19 | 2014-02-19 | 贵州省生物技术研究所 | Production method of bletilla seed stems |
| CN103651121A (en) * | 2013-11-19 | 2014-03-26 | 安徽振扬农林生态开发有限公司 | Bletilla striata differentiation and seedling strengthening medium |
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2014
- 2014-06-13 CN CN201410263929.3A patent/CN104054565B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102283115A (en) * | 2011-06-28 | 2011-12-21 | 陕西科技大学 | Method for quickly reproducing bletilla striata seedlings |
| CN102972290A (en) * | 2012-11-15 | 2013-03-20 | 贵州师范大学 | Method for improving transplanting survival rate of rhizoma bletillae tissue culture seedling |
| CN103583365A (en) * | 2013-11-19 | 2014-02-19 | 贵州省生物技术研究所 | Production method of bletilla seed stems |
| CN103651121A (en) * | 2013-11-19 | 2014-03-26 | 安徽振扬农林生态开发有限公司 | Bletilla striata differentiation and seedling strengthening medium |
Non-Patent Citations (3)
| Title |
|---|
| 白芨组培小苗炼苗移栽简要技术;无;《豆丁网 www.docin.com/p-560887144.html》;20121222;1 * |
| 白芨组培快繁育苗技术研究;袁宁等;《西南农业学报》;20090630;第22卷(第03期);781-785 * |
| 白芨组织培养与快速繁殖技术研究;石云平等;《广西农业科学》;20091231;第40卷(第11期);1408-1410 * |
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