CN104049078A - Combined type biodegradation reaction simulation device - Google Patents

Combined type biodegradation reaction simulation device Download PDF

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Publication number
CN104049078A
CN104049078A CN201410318985.2A CN201410318985A CN104049078A CN 104049078 A CN104049078 A CN 104049078A CN 201410318985 A CN201410318985 A CN 201410318985A CN 104049078 A CN104049078 A CN 104049078A
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water
degradation reaction
degradation
combined type
simulation device
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CN104049078B (en
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王伟
曹媛
刘斌
陈孝政
卢杰妍
刘静
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NANJING INST OF GEOLOGY AND PALEONTOLOGY CHINESE ACADEMY OF SCIENCES
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NANJING INST OF GEOLOGY AND PALEONTOLOGY CHINESE ACADEMY OF SCIENCES
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    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5082Supracellular entities, e.g. tissue, organisms

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Abstract

The invention provides a combined type biodegradation reaction simulation device. According to the combined type biodegradation reaction simulation device, an inner degradation reactor is placed in an external ventilation separation cabinet and is a rectangular semi-sealed or full-sealed device; the inner degradation reactor is internally provided with an internal circulating water power system, a water inlet pump and a temperature control module, and comprises a degradation reaction region I, a water quality detection region II and a hydrodynamic force buffering region III which are separated by a filtering device; the degradation reaction region I is a region for degradation reaction of a living body; the water quality detection region II is internally provided with a series of sensors for monitoring water body parameters in real time; the power of a water pump in the hydrodynamic force buffering region III is slowed down and uniformly dispersed; the external ventilation separation cabinet is internally provided with an exhausting fan and a gas sensor; the temperature control module, an internal circulating water pump, the water inlet pump, the exhausting pump and all the sensors are in communication connection with a general control machine or an upper computer through a control panel so as to realize automatic, online or manual control. The combined type biodegradation reaction simulation device fills up the blank of the prior art; a living body simulated degradation experiment is carried out to obtain the shape, the structure and the environmental change parameters of the living body in a degradation process and an early-stage process of forming fossils.

Description

Combined type biodegradation reaction simulation device
Technical field
The invention belongs to petrifactology and geological experimental facilities field, particularly a kind of relevant to the research of geology petrifactology, with the closely-related biosome degradation experiment of fossil forming process analog machine.
Background technology
Fossil is as establishing geochron, understanding Life Evolution and rebuild palaeoenvironmental important evidence, and its cause analysis receives more and more researchers' concern.Traditional ancient biological study is generally deduced the relevant information of ancient ecologic environment according to existing fossil form, and this research lacks the fully effectively experimental verification of biological characteristic and form relation.And experimental simulation method is by utilizing existing resource simulation to set up various environmental baselines, can verify by experiment inferred extinct plants and animal geological information on the one hand, can in experimentation, seek new breakthrough on the other hand.The method of this experimental simulation is that the new of traditional ancient biological study method supplemented, and also contributes to understand influencing each other of biological and environment under particular surroundings, is the new tool that obtains in the world the research extinct plants and animal geologic media paid attention in the recent period.Numerous research thinks that the formation of fossil is a degraded, deposits the process of burying with mineralising, the significant process of wherein degrading in forming as fossil, and its degradation condition and degree have vital impact to the features such as morphosis of fossil.Experimental simulation method can realize the degradation process of controlled biosome, and can intuitively effectively present biosome and the surrounding environment change procedure under different degradation conditions, for inquiring into fossil formation, provides experimental basis.
Summary of the invention
The object of the present invention is to provide a kind of analog machine of biosome degradation reaction, can provide all types of target degraded environment for bio-carrier simulation, by the observation of biosome simulation degradation process is detected, obtain the form of biosome in degradation process, structure variation and and environmental change information between relation, for the further study fossils origin cause of formation, particularly the history of earth of fossil and the identification mark of biological classification provide the experimental data that has more confidence level and practical significance.
The technical scheme that completes foregoing invention task is, a kind of bio-degradation reactions analogue means, is characterized in that, the main body of this analogue means is become by inner degradation reaction device and external ventilation isolation group; This inside degradation reaction device is placed in external ventilation isolation cabinet, is a rectangle half/whole closing appliance, rigidity impermeable material, consists of; Circulating water power system and water inlet device for supplying in being equipped with in described built-in degradation reaction device; Described built-in degradation reaction device bottom is provided with temperature control module, by heating or freezing, controls the temperature environment in reactor; Described rectangle half/whole closing appliance comprises three regions: degradation reaction district I, water quality detection district II and hydrodynamic force buffer zone III; Between these three regions, be provided with filtration unit, these three zone isolation are opened; Described degradation reaction district I is the region of biosome generation degradation reaction, for biosome being placed on to the inside, carries out degradation reaction and observation; In described water quality detection district II, be equipped with a series of sensors, Real-Time Monitoring water body parameter; The power that water pump produces in the III of hydrodynamic force buffer zone is slowed down with dispersed, the impact for reducing the power of recirculated water on degradation reaction district I; Described temperature control module, interior water circulating pump, intake pump and various water quality sensor are connected with master control machine or upper machine communication by reactor control panel, to realize automatic control, online control or manually to control.
Described interior circulating water power system consists of interior circulation inlet pipeline, interior cyclic water outlet pipeline and interior water circulating pump.Wherein, described interior circulation inlet pipeline and interior cyclic water outlet pipeline, both can exchange, that is and, when interior water circulating pump conversion rotation direction, interior circulation inlet pipeline originally becomes interior cyclic water outlet pipeline; Interior cyclic water outlet pipeline originally becomes interior circulation inlet pipeline; Thereby change water circulation direction.
Described sensor is mainly water quality sensor; Be selected from one or more in lower sensor: temperature sensor, pH sensor, ORP sensor, TDS sensor and/or ion transducer etc.
Described filtration unit is filter screen, filter cloth or solid porous filtering material etc.; The biological residue chip that can prevent degradation reaction district enters water quality detection district and hydrodynamic force buffer zone, avoids affecting water quality detection result and blocking pipe water pump.
The mode of described temperature control module by electroplax heating, electric-heating-wire-heating, water-cooled cooling or semiconductor temperature realizes temperature and controls.
Described water inlet device for supplying comprises intake pump, water inlet pipe and fluid reservoir, and make-up water is delivered to hydrodynamic force buffer zone III from fluid reservoir by water inlet pipe by intake pump, and the physico chemical factor of the make-up water in fluid reservoir needs preparation and stores according to experiment.
Described external ventilation isolation cabinet consists of rigidity impermeable material, and its top is provided with exhaust fan and gas sensor, and this gas sensor is one or more, the NH that may occur for detection of degradation reaction 3, H 2s, SO 2, NO 2; Exhaust fan can open and close according to the data automatic or manual of gas sensor Real-Time Monitoring and feedback; Gas drains into outdoor after exhaust fan is delivered to the purification of external dedicated gas treatment equipment.
Below in conjunction with accompanying drawing, structure of the present invention is done to more detailed explanation:
With reference to Fig. 1: described inside degradation reaction device 1 is the main region that degradation experiment occurs, for target organism carrier provides relatively independent degraded environment.Main body is a rectangle half/whole closing appliance, rigidity impermeable material, consists of.
Described reactor 1 main body mainly comprises three regions: degradation reaction district I, water quality detection district II and hydrodynamic force buffer zone III.
Wherein degradation reaction district I is the region of biosome generation degradation reaction, for biosome being placed on to the inside, carries out degradation reaction and observation;
Wherein water quality detection district II is for analyzing the region of the water quality of catabolite in check degradation process, in water quality detection district, be equipped with a series of sensors 3, Real-Time Monitoring water body parameter, sensor 3 is mainly water quality sensor, comprises temperature sensor, pH sensor, ORP sensor, TDS sensor and conventional ion transducer etc.;
The power that in hydrodynamic force buffer zone III wherein, water pump produces is slowed down with dispersed, reduces as far as possible the impact on degradation reaction district I.Between I and III and I and II, by filtration unit 4, keep apart.Filtration unit 4 is filter screen, filter cloth or solid porous filtering material etc.; The biological residue chip that can prevent degradation reaction district enters water quality detection district and hydrodynamic force buffer zone, avoids affecting water quality detection result and blocking pipe water pump etc.
In the described interior outfit of degradation reaction device 1, circulating water power system mixes to guarantee inner water body, and reduce or the residue that prevents from degrading to the obstruction of filtration unit 4 and the interference to various built-in detecting electrodes;
Described interior circulating water power system consists of circulate in two inlet and outlet pipe 6 and interior water circulating pump 7.Interior water circulating pump 7 is peristaltic pump, can freely change Inlet and outlet water direction, better plays even portions cloth hydrodynamic force and reduces the effect of stopping up.The mouth of pipe of inlet and outlet pipe 6 of circulating in two is separately fixed at top, HeⅡ district, bottom, Ⅲ district, and water circulating pump 7 is placed in inner degradation reaction device 1 outside.
Further optimize, this device has following structure:
Described degradation reaction device 1 upper inside is installed into air pipe 11 and outlet pipeline 12, and air inlet pipeline 11 and outlet pipe 12 are closed according to demand or be open-minded, when experiment needs, can connect with extraneous gas system.11 control gaseous environment for degradation reaction device 1 provides object gas, 12 gas delivery channels exhausts and provide physics and chemistry sense channel and sample for these gases.
In described degradation reaction device, be also provided with into water device for supplying, water inlet device for supplying is from fluid reservoir 10, by water inlet pipe 8, to be delivered to hydrodynamic force buffer zone III by intake pump 9, and the physico chemical factor of the make-up water in fluid reservoir needs preparation and stores according to experiment.
On described degradation reaction device 1 left plate, amount to 6 holes, wherein 5 are passed through by the inlet and outlet pipe 6 that circulates in water inlet pipe 8, air inlet pipeline 11, outlet pipeline 12 and two respectively, separately in middle and upper part, open spout hole 13, prevent that inside reactor liquid level is too high.The cover plate 14 of reactor 1 upper right can freely remove and install, easy to use and maintenance.
Described degradation reaction device 1 can be used as independently reactor and uses separately in fuming cupboard, but also several combine and are placed in the external ventilation isolation a set of parallel connection of the interior formation of cabinet 2 or series connection or reaction system independently separately.
Described external ventilation isolation cabinet 2 main bodys are " ﹄ " shape half/whole closing appliance, rigidity impermeable material, consist of; Object is as inner degradation reaction device 1 provides independent unified analog gas environment, and the issuable gas with foreign flavor of degradation reaction device 1 is detected and discharge processing.
Described external ventilation isolation cabinet 2 top outshots can be the sensor 3, interior water circulating pump 7 of internal reactor 1 etc. placement space are provided.Ventilate and isolate cabinet 2 tops installation exhaust fan 15 and gas sensors 16, exhaust fan 15 objects are the unnecessary gas in continuous or semicontinuous discharge isolation cabinet, and gas sensor 16 is one or more, the NH that may occur for detection of degradation reaction 3, H 2s, SO 2, NO 2deng.Exhaust fan 15 can open and close according to the data automatic or manual of gas sensor 16 Real-Time Monitorings and feedback, and gas drains into outdoor after exhaust fan 15 is delivered to the purification of external dedicated gas treatment equipment.
Breather cheek valve 17 and linker 18 are installed in described external ventilation isolation cabinet 2 right sides.Breather cheek valve 17 is opened when exhaust fan 15 operation, allows outside air to enter in isolation cabinet 2; When exhaust fan 15 cuts out, breather cheek valve 17 sealings.Linker 18, as interface channel, cuts out when the 2 independent use of isolation vent cabinet; When the gas UNICOM of the two or more ventilation isolation of experiment demand cabinet 2, can will isolate cabinet 2 series connection by linker 19 and connect use.
Described external ventilation isolation cabinet 2 left sides are for can open and close hinge door 19, and circle rubber or a sponge sealing strip fixed in hinge door 19 inner sides, guarantees when hinge door 19 is closed to be sealed air-tight state.On isolation cabinet 2 back side panels, open some apertures, facilitate each water pipe and the tracheae of inner degradation reaction device 1 to pass through.
Temperature control module 5 on described degradation reaction device 1, interior water circulating pump 7, intake pump 9 and various water quality sensor 3 are connected with master control machine or host computer 22 communications by reactor control panel 20, can realize automatic control, online control or manually control.
Exhaust fan 15 on described ventilation isolation cabinet 2 is connected with master control machine or host computer 22 communications by shielded box control panel 21 with gas sensor 16, can realize automatic control, online control or manually control.
Described rigidity impermeable material can be organic glass, tempered glass or stainless steel etc.
Wherein on above-mentioned degradation reaction device 1 left plate, limit without fixed measure with the isolation cabinet 2 that ventilates, can adjust according to experiment demand.
The size shape of the hole of wherein opening on above-mentioned degradation reaction device 1 left plate and isolation cabinet 2 back side panels that ventilate, without fixed measure, can relatively freely be adjusted according to demand.
The present invention has filled up the blank of prior art, the biodegradation analogue reactor providing, can provide all types of target degraded environment for biosome simulation, by the observation of biosome simulation degradation process is detected, obtain form, structure and the environmental change parameter of biosome in degradation process, further understand the relation between fossil form and identification mark and the feature of biosome own, and and the fossil mechanism and the physical and chemical processes that form.
Accompanying drawing explanation
Fig. 1 bio-degradation reactions analog machine of the present invention one-piece construction figure.
Fig. 2 is the inside degradation reaction device body design figure of case study on implementation.
Fig. 3 is the external ventilation spacer assembly body design figure of case study on implementation.
Fig. 4 be under aerobic environment and anaerobic environment pH along with the change trend curve of time.Wherein, " ■ " represents aerobic condition, and " ◆ " represents anoxia condition.
Embodiment
By following concrete case study on implementation, further set forth effect feature and the technique effect of degraded simulation reaction equipment, but the present invention is not therefore subject to any restriction.
Embodiment 1, bio-degradation reactions analogue means (referring to Fig. 1), and agent structure is comprised of two parts: inner degradation reaction device (referring to Fig. 2) becomes (referring to Fig. 3) with external ventilation isolation group.
In Fig. 1, inner degradation reaction device 1; External ventilation shielded box 2; Water quality physics and chemistry sensor 3; Filtration unit 4; Temperature control module 5; Interior circulation inlet and outlet pipe 6; Interior water circulating pump 7; Water inlet pipe 8; Water inlet peristaltic pump 9; Fluid reservoir 10; Air inlet pipeline 11; Outlet pipeline 12; Spout hole 13; Dismountable cover plate 14; Exhaust fan 15; Gas sensor 16; Breather cheek valve 17; Linker 18; Hinge door 19; Reactor control panel 20; Shielded box control panel 21; 22 is master control machine or host computer.
Wherein I is degradation reaction district; II is water quality physics and chemistry detection zone or electrode district; III is that hydrodynamic force buffer zone or hydrodynamic force are subdued district.
Inner degradation reaction device main body is the semiclosed device of a rectangle (full-size 270mm*130mm*110mmm, approximately 2.6 liters of useful volume), unorganic glass, consists of.Reactor 1 main body mainly comprises three regions: degradation reaction district I (inside dimension 220mm*100mm*100mm, approximately 2.2 liters of useful volumes), water quality detection district II (approximately 0.2 liter of useful volume) and hydrodynamic force buffer zone III (approximately 0.2 liter of useful volume).Top, water quality detection district has 3 apertures, respectively mounting temperature sensor, pH sensor and dissolved oxygen electrode.Between I and III and I and II, by filtration unit, keep apart.In present case, filtration unit is comprised of iron wire filter screen and loose sponge.The temperature control module of bottom consists of electrical heating wire and metal partion (metp).Interior circulating water power system in degradation reaction device consists of circulate in two inlet and outlet pipe and interior water circulating pump.Interior water circulating pump is peristaltic pump, and the inlet and outlet pipe that circulates in two is silicone tube, and its mouth of pipe is separately fixed at top, HeⅡ district, bottom, Ⅲ district, and it is outside that water circulating pump is placed in inner degradation reaction device, and two emulsion tube pipes pass through by left side hole respectively.On degradation reaction device left plate, amount to 6 holes (Ф 11mm), wherein 5 are passed through by interior circulation inlet and outlet pipe, water inlet pipe, air inlet pipeline and outlet pipeline respectively, separately in middle and upper part, open spout hole, prevent that inside reactor liquid level is too high.The cover plate on degradation reaction device top can freely remove and install.
External ventilation isolation cabinet main body is the semiclosed device of " ﹄ " shape (full-size 430mm*350mm*210mmm, approximately 20 liters of useful volume), organic glass, consists of.External ventilation is isolated portion's outshot perforate cashier's office in a shop (Ф 50mm), in order to exhaust fan to be installed; And open other rectangle hole, for installing gas sensor and control panel circuit etc.Two holes (Ф 30mm) are opened on ventilation isolation cabinet right side, and breather cheek valve and linker are installed.Ventilate isolation cabinet left side for can open and close hinge door, and a circle rubber strip of paper used for sealing bar is fixed in hinge door inner side.On isolation cabinet back side panel, open some apertures (Ф 11mm), facilitate the silica gel water pipe of internal reactor to pass through.
Degradation reaction device is connected with master control machine or upper machine communication by control panel with the isolation various electrical equipment cashier's office in a shop that ventilates, and can realize automatic control and manually control.
Take Fig. 2 and Fig. 3 shown device as analogue reactor main body, investigate the degraded situation of silverfish under aerobic and anoxia condition.
Experiment is divided into two groups of aerobic and anoxics, every group of three parallel experiments, and each experiment is carried out in a degradation reaction device.In aerobic experiment, three degradation reaction devices are placed in the isolation cabinet that ventilates, and experimental anaerobic is the same.Experimental temperature is all 30 ℃, adopts electrical heating wire temperature control.The degradation reaction district I that the clean silverfish (surface is complete, big or small 3.5-3.6cm) of sterilization is placed in to inner degradation reaction device, adds lake water, and cover plate is fastening, and room temperature is placed.An aerator continuous aeration of configuration in each reactor in aerobic experiment, aeration head is placed in hydrodynamic force buffer zone III.In experimentation, Real-Time Monitoring records the water body pH in degradation reaction device, and under the aerobic environment in 1 ~ 6d and oxygen-free environment, pH situation of change as shown in Figure 4.Can find out, in the first six sky that experiment is carried out, As time goes on the pH of water body becomes large, and water body tends to alkalescence, and supposition may be that protein degradation release ammonia causes.Wherein under aerobic condition, pH amplification is greater than anaerobic condition, is because of a large amount of CO that has in anaerobic reactor 2gas dissolution and make water body environment acidifying.

Claims (10)

1. a combined type biodegradation reaction simulation device, is characterized in that, the main body of this analogue means is become with external ventilation isolation group by inner degradation reaction device; This inside degradation reaction device is placed in external ventilation isolation cabinet, is a rectangle half/whole closing appliance, rigidity impermeable material, consists of; Circulating water power system and water inlet device for supplying in being equipped with in described built-in degradation reaction device; Described built-in degradation reaction device bottom is provided with temperature control module, by heating or freezing, controls the temperature environment in reactor; Described rectangle half/whole closing appliance comprises three regions: degradation reaction district I, water quality detection district II and hydrodynamic force buffer zone III; Between these three regions, be provided with filtration unit, these three zone isolation are opened; Described degradation reaction district I is the region of biosome generation degradation reaction, for biosome being placed on to the inside, carries out degradation reaction and observation; In described water quality detection district II, be equipped with a series of sensors, Real-Time Monitoring water body parameter; The power that water pump produces in the III of hydrodynamic force buffer zone is slowed down with dispersed, and the impact for reducing the power of recirculated water on degradation reaction district I guarantees that water quality detection district II can detect the real physical and chemical parameter of reactor simultaneously; Described temperature control module, interior water circulating pump, intake pump and various water quality sensor are connected with master control machine or upper machine communication by reactor control panel, to realize automatic control, online control or manually to control.
2. combined type biodegradation reaction simulation device according to claim 1, is characterized in that, described interior circulating water power system consists of interior circulation inlet pipeline, interior cyclic water outlet pipeline and interior water circulating pump; Wherein, described interior circulation inlet pipeline and interior cyclic water outlet pipeline, both can exchange;
Described interior water circulating pump adopts peristaltic pump; The mouth of pipe of inlet and outlet pipe of circulating in described two is separately fixed at top, HeⅡ district, bottom, Ⅲ district, and it is outside that water circulating pump is placed in inner degradation reaction device.
3. combined type biodegradation reaction simulation device according to claim 1, is characterized in that, described sensor is water quality sensor; Be selected from one or more in lower sensor: temperature sensor, pH sensor, ORP sensor, TDS sensor and/or ion transducer.
4. combined type biodegradation reaction simulation device according to claim 1, is characterized in that, described filtration unit is filter screen, filter cloth or solid porous filtering material.
5. combined type biodegradation reaction simulation device according to claim 1, is characterized in that, described temperature control module is that the mode by electroplax heating, electric-heating-wire-heating, water-cooled cooling or semiconductor temperature realizes temperature and controls.
6. combined type biodegradation reaction simulation device according to claim 1, it is characterized in that, described water inlet device for supplying comprises intake pump, water inlet pipe and fluid reservoir, make-up water is delivered to hydrodynamic force buffer zone III from fluid reservoir by water inlet pipe by intake pump, and the physico chemical factor of the make-up water in fluid reservoir needs preparation and stores according to experiment.
7. combined type biodegradation reaction simulation device according to claim 1, is characterized in that, described degradation reaction device upper inside is provided with air inlet pipeline and outlet pipeline.
8. combined type biodegradation reaction simulation device according to claim 1, is characterized in that, in described degradation reaction device middle and upper part, has opened spout hole, prevents that inside reactor liquid level is too high; On described degradation reaction device top, be provided with the cover plate that can freely remove and install.
9. combined type biodegradation reaction simulation device according to claim 1, it is characterized in that, described degradation reaction device can be used as independently reactor and uses separately in fuming cupboard, or the degradation reaction device described in several combines to be placed in and forms a set of parallel connection or series connection or reaction system independently separately in external ventilation isolation cabinet, to be applicable to different experimental study requirements.
10. according to the combined type biodegradation reaction simulation device one of claim 1-9 Suo Shu, it is characterized in that, described external ventilation isolation cabinet consists of rigidity impermeable material, its top is provided with exhaust fan and gas sensor, this gas sensor is one or more, the NH that may occur for detection of degradation reaction 3, H 2s, SO 2, NO 2; Exhaust fan opens and closes according to the data automatic or manual of gas sensor Real-Time Monitoring and feedback; Gas drains into outdoor after exhaust fan is delivered to the purification of external dedicated gas treatment equipment.
CN201410318985.2A 2014-07-04 2014-07-04 Combined type biodegradation reaction simulation device Expired - Fee Related CN104049078B (en)

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CN112525975A (en) * 2020-12-10 2021-03-19 重庆锟铻电子科技有限公司 Humidity sensor special for biodegradable toilet bowl

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CN112525975A (en) * 2020-12-10 2021-03-19 重庆锟铻电子科技有限公司 Humidity sensor special for biodegradable toilet bowl

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