CN203275260U - Constant temperature anaerobic reactor - Google Patents
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- CN203275260U CN203275260U CN 201320261181 CN201320261181U CN203275260U CN 203275260 U CN203275260 U CN 203275260U CN 201320261181 CN201320261181 CN 201320261181 CN 201320261181 U CN201320261181 U CN 201320261181U CN 203275260 U CN203275260 U CN 203275260U
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- 239000007788 liquid Substances 0.000 claims abstract description 28
- 238000006243 chemical reaction Methods 0.000 claims abstract description 24
- 239000002699 waste material Substances 0.000 claims abstract description 22
- 238000011084 recovery Methods 0.000 claims abstract description 9
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 6
- 238000007789 sealing Methods 0.000 claims abstract 2
- 230000002572 peristaltic effect Effects 0.000 claims description 13
- 239000007789 gas Substances 0.000 claims description 5
- 239000011261 inert gas Substances 0.000 claims description 5
- 239000012295 chemical reaction liquid Substances 0.000 claims description 3
- 238000002848 electrochemical method Methods 0.000 claims description 2
- 206010037660 Pyrexia Diseases 0.000 claims 2
- 239000000376 reactant Substances 0.000 claims 2
- NJPPVKZQTLUDBO-UHFFFAOYSA-N novaluron Chemical compound C1=C(Cl)C(OC(F)(F)C(OC(F)(F)F)F)=CC=C1NC(=O)NC(=O)C1=C(F)C=CC=C1F NJPPVKZQTLUDBO-UHFFFAOYSA-N 0.000 claims 1
- 230000007613 environmental effect Effects 0.000 abstract description 26
- 238000010438 heat treatment Methods 0.000 abstract description 15
- 238000005260 corrosion Methods 0.000 abstract description 10
- 230000007797 corrosion Effects 0.000 abstract description 10
- 244000005700 microbiome Species 0.000 abstract description 10
- 239000002184 metal Substances 0.000 abstract description 7
- 229910052751 metal Inorganic materials 0.000 abstract description 7
- 229910052760 oxygen Inorganic materials 0.000 description 7
- 239000001301 oxygen Substances 0.000 description 7
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 6
- 241000894006 Bacteria Species 0.000 description 4
- 230000000694 effects Effects 0.000 description 3
- 230000000813 microbial effect Effects 0.000 description 3
- 241001148471 unidentified anaerobic bacterium Species 0.000 description 3
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 2
- 238000000034 method Methods 0.000 description 2
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- 241001148470 aerobic bacillus Species 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
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- 229910002092 carbon dioxide Inorganic materials 0.000 description 1
- 239000001569 carbon dioxide Substances 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000006056 electrooxidation reaction Methods 0.000 description 1
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- 239000007787 solid Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000008399 tap water Substances 0.000 description 1
- 235000020679 tap water Nutrition 0.000 description 1
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Abstract
本实用新型为一种恒温厌氧反应器，包括反应装置、通过导管与反应装置相连的加液装置和废液回收装置，其中反应装置包括恒温池以及置于恒温池内的环境池，环境池为密封性结构，内置反应液，恒温池与环境池接触的基座上设置有发热片，通过加热恒温池内的水来保持环境池内反应液的温度，通过加液装置向环境池内加入新鲜溶液，排除废液至废液回收装置起到更新的作用。本实用新型具有恒温、厌氧、自动更新或循环的作用，适用于研究厌氧微生物对金属腐蚀的影响。
The utility model relates to a constant temperature anaerobic reactor, which includes a reaction device, a liquid addition device connected to the reaction device through a conduit, and a waste liquid recovery device, wherein the reaction device includes a constant temperature pool and an environmental pool placed in the constant temperature pool. The environmental pool is Sealing structure, built-in reaction solution, heating sheet is set on the base where the constant temperature pool and the environmental pool are in contact, the temperature of the reaction solution in the environmental pool is maintained by heating the water in the constant temperature pool, and fresh solution is added to the environmental pool through the liquid adding device to eliminate The waste liquid to the waste liquid recovery device plays a role of renewal. The utility model has the functions of constant temperature, anaerobic, automatic renewal or circulation, and is suitable for studying the influence of anaerobic microorganisms on metal corrosion.
Description
技术领域 technical field
本发明涉及微生物培养和金属腐蚀测量设备领域，具体地来说为一种用于恒温、厌氧、自动更新或循环的恒温厌氧反应器。 The invention relates to the field of microbial cultivation and metal corrosion measuring equipment, in particular to a constant temperature anaerobic reactor for constant temperature, anaerobic, automatic renewal or circulation.
背景技术 Background technique
在石油、化工、机械等领域，微生物被发现是影响金属腐蚀的重要原因之一，尤其是在水环境中。能引起材料腐蚀和降解的微生物包括好氧和厌氧细菌，其中，厌氧细菌中的硫酸盐还原菌被认为是与金属腐蚀相关的主要细菌，深入研究厌氧细菌对金属腐蚀的影响及作用机制具有重大的理论和实际意义。 In the fields of petroleum, chemical industry, machinery, etc., microorganisms have been found to be one of the important reasons affecting metal corrosion, especially in water environments. Microorganisms that can cause material corrosion and degradation include aerobic and anaerobic bacteria. Among them, sulfate-reducing bacteria in anaerobic bacteria are considered to be the main bacteria related to metal corrosion. In-depth research on the influence and effect of anaerobic bacteria on metal corrosion The mechanism has great theoretical and practical significance.
厌氧微生物通常认为这是一类只能在低氧分压的条件下生长，而不能在空气（18%氧气）和/或10%二氧化碳浓度下的固体培养基表面生长的微生物，一般的厌氧微生物在自然界生存于常温的无氧和少氧环境中，不同的温度环境下厌氧微生物对金属的腐蚀程度是不同的，因此在研究厌氧微生物的代谢机制时，需要保持恒温、厌氧的环境。 Anaerobic microorganisms are generally considered to be a type of microorganisms that can only grow under low oxygen partial pressure conditions, but cannot grow on the surface of solid medium in air (18% oxygen) and/or 10% carbon dioxide concentration. Aerobic microorganisms live in anaerobic and low-oxygen environments at room temperature in nature. The degree of corrosion of anaerobic microorganisms to metals is different under different temperature environments. Therefore, when studying the metabolic mechanism of anaerobic microorganisms, it is necessary to maintain a constant temperature, anaerobic environment of.
发明内容 Contents of the invention
针对现有技术中存在的问题，本发明要解决的问题在于提供一种具有恒温、厌氧和自动更新或循环的恒温厌氧反应器。 Aiming at the problems existing in the prior art, the problem to be solved by the present invention is to provide a constant temperature anaerobic reactor with constant temperature, anaerobic and automatic renewal or circulation.
本发明采用如下的技术方案： The present invention adopts following technical scheme:
一种恒温厌氧反应器，包括反应装置、通过导管与反应装置相连的加液装置和废液回收装置，其中反应装置包括恒温池以及置于恒温池内的环境池，环境池为密封性结构，内置反应液，恒温池与环境池相连的基座上设置有发热片，通过加热恒温池内的水来保持环境池内反应液的温度。 A constant temperature anaerobic reactor, comprising a reaction device, a liquid addition device and a waste liquid recovery device connected to the reaction device through a conduit, wherein the reaction device includes a constant temperature pool and an environmental pool placed in the constant temperature pool, the environmental pool is a sealed structure, The built-in reaction liquid, the base connected to the constant temperature pool and the environmental pool is provided with a heating sheet, and the temperature of the reaction liquid in the environmental pool is maintained by heating the water in the constant temperature pool.
所述环境池顶部设置有进气口和出气口。 An air inlet and an air outlet are arranged on the top of the environmental pool.
所述环境池顶部设置有伸入反应液内的辅助电极、参比电极以及工作电极，用于连接外部的电化学测量设备。 An auxiliary electrode, a reference electrode and a working electrode protruding into the reaction solution are arranged on the top of the environmental pool for connecting external electrochemical measuring equipment.
所述反应装置安装有温度控制开关，温度控制开关输出端与发热片连接，温度控制开关的输入端连接有温度传感器，温度传感器内置于恒温池内，温度控制开关通过比较温度传感器传至的温度信号与设置的信号，控制电源的通断，进而控制发热片的加热状态，从而达到恒温效果。 The reaction device is equipped with a temperature control switch, the output end of the temperature control switch is connected to the heating sheet, the input end of the temperature control switch is connected to a temperature sensor, the temperature sensor is built in the constant temperature pool, and the temperature control switch is compared with the temperature signal transmitted by the temperature sensor With the set signal, control the on and off of the power supply, and then control the heating state of the heating sheet, so as to achieve a constant temperature effect.
所述加液装置包括蠕动泵和新鲜溶液池，蠕动泵置于反应装置与新鲜溶液池之间，以一定的速率将新鲜溶液压入环境池内，所述蠕动泵流量范围在1.0mL/h和20L/h范围内可调。 The liquid adding device includes a peristaltic pump and a fresh solution pool. The peristaltic pump is placed between the reaction device and the fresh solution pool to press the fresh solution into the environmental pool at a certain rate. The flow range of the peristaltic pump is between 1.0mL/h and 20L /h range adjustable.
所述新鲜溶液池的顶部设置有出气口和进气口，用于充入惰性气体或实验要求的特殊气体。 The top of the fresh solution pool is provided with an air outlet and an air inlet for filling inert gas or special gas required by the experiment.
所述废液回收装置为废液储藏瓶，通过废液导管与环境池相连。 The waste liquid recovery device is a waste liquid storage bottle, which is connected to the environmental pool through a waste liquid conduit.
本发明具有如下的优点和有益效果： The present invention has following advantage and beneficial effect:
1. 本发明通过发热片加热水浴池中的自来水，并通过温度传感器和温度开关自动控制电源的开合，达到恒定温度的效果；根据实验要求实验温度可以在室温到100℃的范围可控，普遍适用于研究微生物腐蚀。 1. The present invention heats the tap water in the water bath through the heating plate, and automatically controls the opening and closing of the power supply through the temperature sensor and the temperature switch to achieve the effect of constant temperature; according to the experimental requirements, the experimental temperature can be controlled from room temperature to 100 ℃, It is generally applicable to the study of microbial corrosion.
2. 本发明通过蠕动泵对环境池中的溶液进行更新或循环，蠕动泵流量范围在1.0mL/h和20L/h范围内可调。通过溶液的更新可以维持细菌量在一定的水平，而不会发生细菌因营养物耗尽而衰亡。另外，通过控制流量的大小，也可以大体上控制细菌菌落的总数。 2. The present invention updates or circulates the solution in the environmental pool through the peristaltic pump, and the flow range of the peristaltic pump is adjustable within the range of 1.0mL/h and 20L/h. The amount of bacteria can be maintained at a certain level through the renewal of the solution, and the bacteria will not die due to the depletion of nutrients. In addition, by controlling the size of the flow, the total number of bacterial colonies can also be generally controlled.
3. 本发明设置了废液储藏瓶，避免了废液直接流向环境中污染环境的问题，符合环保要求；另外定时取出废液并对废液进行离子检测也能评价环境中的离子浓度的改变，进一步研究微生物腐蚀发生的原因。 3. The present invention is equipped with a waste liquid storage bottle, which avoids the problem of waste liquid directly flowing into the environment and pollutes the environment, and meets the requirements of environmental protection; in addition, taking out the waste liquid regularly and performing ion detection on the waste liquid can also evaluate the change of ion concentration in the environment , to further study the cause of microbial corrosion.
4. 本发明通过向新鲜溶液池和环境池中充入惰性气体既可以排除环境池和新鲜溶液池中的氧气，又保持了环境池内气压的平衡，特别适合于研究厌氧微生物对金属腐蚀的影响。 4. The present invention can not only eliminate the oxygen in the environment pool and the fresh solution pool by filling the fresh solution pool and the environment pool with inert gas, but also maintain the balance of air pressure in the environment pool, and is especially suitable for studying the effect of anaerobic microorganisms on metal corrosion Influence.
5. 通过安置在环境池中的电化学体系（工作电极、参比电极和辅助电极）可以对样品的状态进行电化学测量，对发生在环境池的电化学腐蚀过程进行实时检测。 5. Through the electrochemical system (working electrode, reference electrode and auxiliary electrode) placed in the environmental pool, the state of the sample can be electrochemically measured, and the electrochemical corrosion process in the environmental pool can be detected in real time.
附图说明 Description of drawings
图1为本发明的结构示意图； Fig. 1 is a structural representation of the present invention;
图中，1为新鲜溶液池； 2为气体导管；3为硅质胶管；4为蠕动泵； 5为环境池；6为进气口；7为温度计；8为工作电极；9为参比电极；10为辅助电极；11为出气口；12为废液导管；13为废液储藏瓶；14为恒温池 ；15为发热片；16为温度传感器；17为温度控制开关。
In the figure, 1 is a fresh solution pool; 2 is a gas conduit; 3 is a silicon rubber tube; 4 is a peristaltic pump; 5 is an environmental pool; 6 is an air inlet; 7 is a thermometer; 8 is a working electrode; 9 is a
具体实施方式 Detailed ways
下面结合附图对本发明结构进行详细地说明。 The structure of the present invention will be described in detail below in conjunction with the accompanying drawings.
实施例 Example
如图1所示，本实施例恒温厌氧反应器，包括反应装置、加液装置和废液回收装置，加液装置和废液回收装置与反应装置之间通过导管连接，加液装置向反应装置内加入新鲜溶液，反应装置内的废液通过导管流入废液回收装置内，其中反应装置包括恒温池14以及置于恒温池14内的环境池5，环境池5为密封性结构，内均有厌氧微生物的反应液，环境池5可以完全或部分浸入恒温池14内，恒温池14与环境池5接触的基座上设置有发热片15，通过加热恒温池14内的水来保持环境池5内反应液的恒温。
As shown in Figure 1, the constant temperature anaerobic reactor of this embodiment includes a reaction device, a liquid addition device and a waste liquid recovery device. Fresh solution is added into the device, and the waste liquid in the reaction device flows into the waste liquid recovery device through the conduit, wherein the reaction device includes a
为了平衡环境池5内的气压以及排除氧气，在环境池5顶部设置有进气口6和出气口11，通入惰性气体来排除环境池5内的氧气；在环境5的上还设置有温度计7，检测环境池内的温度变化。
In order to balance the air pressure in the
环境池5顶部设置有深入反应液内的辅助电极10、参比电极9以及工作电极8，用于连接外部的电化学测量设备，可以对样品的状态进行电化学测量。
The top of the
反应装置安装有温度控制开关17，温度控制开关17输出端与发热片连接，温度控制开关的输入端连接有温度传感器16，温度传感器16内置于恒温池内，温度控制开关通过比较温度传感器传至的温度信号与设置的信号，控制电源的通断，进而控制发热片的加热状态，从而达到恒温效果。
The reaction device is equipped with a
加液装置包括蠕动泵4和新鲜溶液池1，蠕动泵4置于反应装置与新鲜溶液池之间将新鲜溶液加入环境池内，蠕动泵4流量范围在1.0mL/h和20L/h范围内可调，蠕动泵4通过硅质胶管3与新鲜溶液池1连接。
The liquid adding device includes a peristaltic pump 4 and a
新鲜溶液池1为密封结构，新鲜溶液1的顶部设置有出气口和进气口，通过气体导管2充入惰性气体来平衡内部压力以及排除氧气。
The
本实施例中溶液回收装置为废液储藏瓶13，通过废液导管12与环境池相连。
In this embodiment, the solution recovery device is a waste
实施例2 Example 2
与实施例1的不同之处在于，发热片位于恒温池的四周。 The difference from Example 1 is that the heating sheet is located around the constant temperature pool. the
Claims (7)
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104155236A (en) * | 2013-05-14 | 2014-11-19 | 中国科学院金属研究所 | Thermostat anaerobic reactor |
| CN106830309A (en) * | 2017-02-20 | 2017-06-13 | 中冶南方工程技术有限公司 | Permanent moderate temperature UASB reactor |
| CN112973482A (en) * | 2021-04-08 | 2021-06-18 | 上海海洋大学 | Dansyl chloride derivative solution dissolving method and reagent bottle assembly |
-
2013
- 2013-05-14 CN CN 201320261181 patent/CN203275260U/en not_active Expired - Fee Related
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104155236A (en) * | 2013-05-14 | 2014-11-19 | 中国科学院金属研究所 | Thermostat anaerobic reactor |
| CN106830309A (en) * | 2017-02-20 | 2017-06-13 | 中冶南方工程技术有限公司 | Permanent moderate temperature UASB reactor |
| CN112973482A (en) * | 2021-04-08 | 2021-06-18 | 上海海洋大学 | Dansyl chloride derivative solution dissolving method and reagent bottle assembly |
| CN112973482B (en) * | 2021-04-08 | 2025-06-24 | 上海海洋大学 | A method for dissolving dansyl chloride derivative solution and a reagent bottle assembly |
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