CN104046590B - 一种稀少精子体外培养液 - Google Patents
一种稀少精子体外培养液 Download PDFInfo
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- CN104046590B CN104046590B CN201410270739.4A CN201410270739A CN104046590B CN 104046590 B CN104046590 B CN 104046590B CN 201410270739 A CN201410270739 A CN 201410270739A CN 104046590 B CN104046590 B CN 104046590B
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- sperm
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- oligospermia
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- 238000000338 in vitro Methods 0.000 title claims abstract description 42
- 206010003883 azoospermia Diseases 0.000 title claims abstract description 21
- 206010067162 Asthenospermia Diseases 0.000 title abstract 3
- 208000008634 oligospermia Diseases 0.000 title abstract 3
- 230000036616 oligospermia Effects 0.000 title abstract 3
- 231100000528 oligospermia Toxicity 0.000 title abstract 3
- 239000001963 growth medium Substances 0.000 claims abstract description 28
- 210000001550 testis Anatomy 0.000 claims abstract description 14
- DAEPDZWVDSPTHF-UHFFFAOYSA-M sodium pyruvate Chemical compound [Na+].CC(=O)C([O-])=O DAEPDZWVDSPTHF-UHFFFAOYSA-M 0.000 claims abstract description 10
- XOAAWQZATWQOTB-UHFFFAOYSA-N taurine Chemical compound NCCS(O)(=O)=O XOAAWQZATWQOTB-UHFFFAOYSA-N 0.000 claims abstract description 10
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 claims abstract description 8
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims abstract description 8
- WCUXLLCKKVVCTQ-UHFFFAOYSA-M Potassium chloride Chemical compound [Cl-].[K+] WCUXLLCKKVVCTQ-UHFFFAOYSA-M 0.000 claims abstract description 8
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims abstract description 8
- LWIHDJKSTIGBAC-UHFFFAOYSA-K tripotassium phosphate Chemical compound [K+].[K+].[K+].[O-]P([O-])([O-])=O LWIHDJKSTIGBAC-UHFFFAOYSA-K 0.000 claims abstract description 8
- 210000000918 epididymis Anatomy 0.000 claims abstract description 6
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- MTCFGRXMJLQNBG-REOHCLBHSA-N (2S)-2-Amino-3-hydroxypropansäure Chemical compound OC[C@H](N)C(O)=O MTCFGRXMJLQNBG-REOHCLBHSA-N 0.000 claims abstract description 5
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims abstract description 5
- BYPFEZZEUUWMEJ-UHFFFAOYSA-N Pentoxifylline Chemical compound O=C1N(CCCCC(=O)C)C(=O)N(C)C2=C1N(C)C=N2 BYPFEZZEUUWMEJ-UHFFFAOYSA-N 0.000 claims abstract description 5
- 239000008103 glucose Substances 0.000 claims abstract description 5
- 229940054269 sodium pyruvate Drugs 0.000 claims abstract description 5
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- CKLJMWTZIZZHCS-UHFFFAOYSA-N D-OH-Asp Natural products OC(=O)C(N)CC(O)=O CKLJMWTZIZZHCS-UHFFFAOYSA-N 0.000 claims abstract description 4
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- CEAZRRDELHUEMR-URQXQFDESA-N Gentamicin Chemical compound O1[C@H](C(C)NC)CC[C@@H](N)[C@H]1O[C@H]1[C@H](O)[C@@H](O[C@@H]2[C@@H]([C@@H](NC)[C@@](C)(O)CO2)O)[C@H](N)C[C@@H]1N CEAZRRDELHUEMR-URQXQFDESA-N 0.000 claims abstract description 4
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- CKLJMWTZIZZHCS-UWTATZPHSA-N L-Aspartic acid Natural products OC(=O)[C@H](N)CC(O)=O CKLJMWTZIZZHCS-UWTATZPHSA-N 0.000 claims abstract description 4
- ONIBWKKTOPOVIA-BYPYZUCNSA-N L-Proline Chemical compound OC(=O)[C@@H]1CCCN1 ONIBWKKTOPOVIA-BYPYZUCNSA-N 0.000 claims abstract description 4
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- ZDXPYRJPNDTMRX-VKHMYHEASA-N L-glutamine Chemical compound OC(=O)[C@@H](N)CCC(N)=O ZDXPYRJPNDTMRX-VKHMYHEASA-N 0.000 claims abstract description 4
- BELBBZDIHDAJOR-UHFFFAOYSA-N Phenolsulfonephthalein Chemical compound C1=CC(O)=CC=C1C1(C=2C=CC(O)=CC=2)C2=CC=CC=C2S(=O)(=O)O1 BELBBZDIHDAJOR-UHFFFAOYSA-N 0.000 claims abstract description 4
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- MKJXYGKVIBWPFZ-UHFFFAOYSA-L calcium lactate Chemical compound [Ca+2].CC(O)C([O-])=O.CC(O)C([O-])=O MKJXYGKVIBWPFZ-UHFFFAOYSA-L 0.000 claims abstract description 4
- 239000001527 calcium lactate Substances 0.000 claims abstract description 4
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- 239000011780 sodium chloride Substances 0.000 claims abstract description 4
- 235000002639 sodium chloride Nutrition 0.000 claims abstract description 4
- UIIMBOGNXHQVGW-UHFFFAOYSA-M Sodium bicarbonate Chemical compound [Na+].OC([O-])=O UIIMBOGNXHQVGW-UHFFFAOYSA-M 0.000 claims description 6
- 229930182816 L-glutamine Natural products 0.000 claims description 3
- 235000017557 sodium bicarbonate Nutrition 0.000 claims description 3
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- ZDXPYRJPNDTMRX-UHFFFAOYSA-N glutamine Natural products OC(=O)C(N)CCC(N)=O ZDXPYRJPNDTMRX-UHFFFAOYSA-N 0.000 abstract 1
- 229960002743 glutamine Drugs 0.000 abstract 1
- 229960002449 glycine Drugs 0.000 abstract 1
- 229960003390 magnesium sulfate Drugs 0.000 abstract 1
- 229960002816 potassium chloride Drugs 0.000 abstract 1
- 229940093916 potassium phosphate Drugs 0.000 abstract 1
- 229960002429 proline Drugs 0.000 abstract 1
- 229960001153 serine Drugs 0.000 abstract 1
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RU2603084C2 (ru) * | 2015-03-18 | 2016-11-20 | Федеральное государственное бюджетное учреждение "Научный центр акушерства, гинекологии и перинатологии имени академика В.И. Кулакова" Министерства здравоохранения Российской Федерации | Способ дифференцировки живых сперматозоидов с помощью диагностической культуральной среды при абсолютной астенозооспермии в рамках экстракорпорального оплодотворения |
CN108531446B (zh) * | 2018-03-28 | 2021-08-31 | 成都艾伟孚生物科技有限公司 | 一种精子梯度离心液及其配制方法 |
CN108315295A (zh) * | 2018-03-28 | 2018-07-24 | 南宁市第二人民医院 | 睾丸穿刺精子的培养液 |
CN109136172B (zh) * | 2018-09-11 | 2022-02-11 | 苏州市立医院 | 提高囊胚率的小鼠精子体外培养试剂盒及其使用方法 |
CN110402919B (zh) * | 2019-07-17 | 2021-09-28 | 西北农林科技大学 | 环腺苷酸用于制备山羊精子冷冻保存用稀释液的应用及制备方法 |
CN110923200B (zh) * | 2019-12-25 | 2021-08-03 | 佛山辅康生物科技有限公司 | 一种精子培养液及其制备方法 |
CN111345284B (zh) * | 2020-04-09 | 2021-04-20 | 四川大学华西第二医院 | 一种人类睾丸组织悬液冷冻保护剂及其制备方法 |
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JP3660026B2 (ja) * | 1995-09-04 | 2005-06-15 | 扶桑薬品工業株式会社 | 体外受精用培地組成物 |
US6140121A (en) * | 1995-10-19 | 2000-10-31 | Advanced Reproduction Technologies, Inc. | Methods and compositions to improve germ cell and embryo survival and function |
TR200101538T2 (tr) * | 1998-11-30 | 2001-12-21 | Ivf Sciences Colorado, Inc. | In vitro dölleme sistemi ve sıralı kültür ortamı. |
RU2007143058A (ru) * | 2005-04-21 | 2009-05-27 | Гленн А. ГОЛДШТЕЙН (US) | Амид n-ацетилцистеина (амид nac) для лечения окислительного стресса, связанного с бесплодием |
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Effective date of registration: 20220617 Address after: 201900 2-3 / F, No.5, Lane 18, Weihang Road, Baoshan District, Shanghai Patentee after: SHANGHAI BEION PHARMACEUTICAL TECHNOLOGY CO.,LTD. Address before: 200127 room 210, zone I, floor 2, building 4, No. 264, Liantang new West Road, Qingpu District, Shanghai Patentee before: GAOHONG BIOTECHNOLOGY (SHANGHAI) CO.,LTD. |
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Denomination of invention: A rare sperm in vitro culture medium Granted publication date: 20170201 Pledgee: Industrial Bank Co.,Ltd. Shanghai Pengpu Sub branch Pledgor: SHANGHAI BEION PHARMACEUTICAL TECHNOLOGY CO.,LTD. Registration number: Y2024310000281 |
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