CN104041521B - The application in field produces of water-retaining agent and Nicotiana tabacum L. Promoting bacteria - Google Patents
The application in field produces of water-retaining agent and Nicotiana tabacum L. Promoting bacteria Download PDFInfo
- Publication number
- CN104041521B CN104041521B CN201410115221.3A CN201410115221A CN104041521B CN 104041521 B CN104041521 B CN 104041521B CN 201410115221 A CN201410115221 A CN 201410115221A CN 104041521 B CN104041521 B CN 104041521B
- Authority
- CN
- China
- Prior art keywords
- water
- retaining agent
- promoting bacteria
- promoting
- soil
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses water-retaining agent and the application in field produces jointly of tobacco seedlings Promoting bacteria, this water-retaining agent has the effect that prolongation Promoting bacteria is grown in soil surely, grow owing to extending Promoting bacteria determining in soil, serving more preferable growth-promoting effect, the cigarette strain growing way that Promoting bacteria water-retaining agent processes is considerably better than comparison.
Description
Technical field
The invention belongs to microorganism and agricultural production, relate to water-retaining agent and Nicotiana tabacum L. Promoting bacteria answering in field produces
With.
Background technology
Water-retaining agent also known as high water absorbent, wetting agent, super-strong moisture absorbing resin, hydrophilic intelligence multipotency cross-polymer etc., be utilize superpower
A kind of functional high-polymer polymer with super-strong moisture absorbing water holding capacity that water-absorbing resins is made.This kind of material contains in a large number
The group that absorbs water by force that structure is special, it can absorb the moisture of own wt hundred times.Owing to molecular structure cross-links, molecular network institute
Water suction point can not be extruded with general physical method, therefore has the strongest water retention property.Water-retaining agent has absorbent function repeatedly, water suction
After be expanded to hydrogel, moisture slowly can be discharged for crop utilization the most again.Water-retaining agent can strengthen soil water-retaining
Property, improve Soil structure, improve rainfall infiltration rate, reduce soil moisture and the loss of nutrient, improve rainfall, irrigation water and soil
The utilization rate of accumulation water.In addition water-retaining agent also has the features such as selection absorbency, tolerance to cold, water penetration, slow-releasing, dilatancy.
Water-retaining agent is widely used in soil conditioner, many studies have shown that, uses water-retaining agent and can improve soil thing in soil
Reason moisture character, increases soil water retention, increases the adsorption to fertilizer, thus reaches to promote that crop emerges, survives, improves
Crop dry-matter accumulation, promotes the purpose of root system development.Water-retaining agent can also be as culture matrix for the crop such as flowers, vegetable
Nursery.The result of study on Fructus Cucumidis sativi such as Zhao Rui shows, adds water-retaining agent and significantly improve the confession of substrate in seedling medium
Aqueous condition, promotes the root growth and development of rice shoot, improves the physiologically active of rice shoot, is beneficial to form strong sprout and can improve Huang
The yield of melon.
Along with the high speed development of industry, environmental pollution is further exacerbated by, and the environmental consciousness of people is strengthened day by day, thirsts for finding
A kind of usage amount that can not only reduce pesticide and chemical fertilizer, can not also affect the effective means even promoting plant growing simultaneously.
Under such realistic background, to plant rhizosphere growth-promoting antibacterial PGPR (Plant Growth Promoting
Rhizobacteria) research increasingly receives publicity.The states such as the U.S., Japan, Australia all expand the special project to PGPR
Research, PGPR is increasingly becoming international study hotspot.
But how to extend Promoting bacteria and determine, in field, the time of growing, give full play to its effect be those skilled in the art urgently
Solve the technical problem that.
Summary of the invention
It is an object of the invention to the above-mentioned deficiency overcome existing for prior art, one aspect of the present invention relates to water-retaining agent and cigarette
The application in field produces of the grass Promoting bacteria, on the other hand, further relates to Nicotiana tabacum L. Promoting bacteria itself.In order to realize the mesh of the present invention
, intend adopting the following technical scheme that
The present invention relates to water-retaining agent and the application in field produces jointly of Nicotiana tabacum L. Promoting bacteria.
In a preferred embodiment of the present invention, described Nicotiana tabacum L. Promoting bacteria preserving number is CGMCC NO.'s 8266
Bacillus licheniformis or its direct transfer thing.
In another preferred embodiment of the present invention, described field refers to tobacco planting field.
In a preferred embodiment of the present invention, described water-retaining agent is propylene hydrochloric acid-acrylic amide water-retaining agent
(BJ2101), purchased from Beijing Han Limiao Technew SA.
In another aspect of this invention, the invention still further relates to described Nicotiana tabacum L. Promoting bacteria.
In another aspect of this invention, the invention still further relates to water-retaining agent and surely grow answering in the time at prolongation Nicotiana tabacum L. Promoting bacteria
With.
Accompanying drawing explanation
Fig. 1 growth-promoting microorganism growth-promoting functions to tobacco seedlings；
Fig. 2 Promoting bacteria quantity dynamic monitoring in water-retaining agent；
Promoting bacteria Number dynamics change in water-retaining agent in Fig. 3 soil；
Fig. 4 water-retaining agent extends Promoting bacteria and surely grows to promote that cigarette strain grows；
Microbial information
Tobacco seedlings Promoting bacteria LX5 lichens bud used in a preferred embodiment of the present invention and detailed description of the invention
Spore bacillus (Bacillus licheniformis) it is preserved in the common micro-life of China Committee for Culture Collection of Microorganisms
Thing center, deposit number CGMCC NO. 8266, preservation address is: Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3, section of China
Institute of microbiology of institute, preservation date is JIUYUE in 2013 25.
Detailed description of the invention
With specific embodiment, the method for the present invention is described in more detail below in conjunction with the accompanying drawings.
One, the screening of tobacco seedlings Promoting bacteria microorganism
By fertilizers such as the oil cake fertilizer after fermentation, cattle manure fertilizers by the separation of dilution plate rubbing method, pure culture
Antibacterial, fungus and actinomycetes in fertilizer, picking list bacterium colony carries out DNA qualification.Select different types of microorganism respectively cattle
Meat extract protein culture medium (Carnis Bovis seu Bubali cream 5.0 g, sodium chloride 5.0 g, peptone 10 g, agar 20 g, distilled water 1000 mL,
PH 7.0-7.2,121 DEG C of sterilizing 20 min, for the cultivation of antibacterial), Gause I culture medium (potassium nitrate 1.0 g, phosphoric acid hydrogen
Dipotassium 0.5 g, sodium chloride 0.5 g, magnesium sulfate 0.5 g, ferrous sulfate 0.01 g, soluble starch 20.0 g, agar 20.0 g,
Sterilized water 1000 mL, pH value 7.2 ~ 7.4,121 DEG C of sterilizing 20 min, for actinomycetic cultivation) and Martin-rose-bengal
Culture medium (glucose 10 g, peptone 5 g, K2HPO4 1 g, MgSO4.7H2O 0.5 g, 1/300 rose-bengal aqueous solution
10 mL, agar 20.0 g, sterilized water 1000 mL, pH are natural, and 115 DEG C of sterilizing 30 min, for the cultivation of fungus) on carry out
Purification.By comparing whether tobacco seedling growth is had facilitation to determine growth-promoting microorganism by the strain screened.Select tobacco seedlings raw
Long there is notable growth-promoting functions LX5 Promoting bacteria (Bacillus licheniformisBacillus licheniformis, Chinese micro-life
Thing culture presevation administration committee's common micro-organisms center CGMCC NO. 8266), this Promoting bacteria main biological property is: leather
Blue Albert'stain Albert positive G+, cellular morphology is shaft-like, Dan Sheng, and spore is oval or cylindrical, without pod membrane, can move, facultative good
Oxygen, bacterium colony is opaque, in irregular shape, adhesion, rough surface.Can make gelatin liquefaction, Starch Hydrolysis, V.P. tests the positive, profit
Use citrate.This strain apply after compared with the control, increase tobacco root system vigor 1.08 times, increase root dry weight 16.2 %, cigarette
Leaf-making quantity improves 15.5 % with comparing, and utilization rate of fertilizer improves 10 more than %.
T1 in Fig. 1 is Routine control, is added without any strain, and T2-T8 adds 10 mL2-8 respectively after tobacco seedlings is transplanted
Number Promoting bacteria 36 h fermentation liquid (109Cfu/mL), wherein T5 processes for adding LX5 Promoting bacteria.T5 is can be seen that by result
Reason Biomass is significantly higher than comparison T1, it was demonstrated that LX5 Promoting bacteria has significant facilitation to tobacco seedling growth, and in all rush
During raw bacterium processes, growth-promoting effect is the strongest.
Two, tobacco seedlings Promoting bacteria determining in water-retaining agent is grown
The tobacco seedlings Promoting bacteria LX5 screened is inoculated in beef extract-peptone fluid medium, 170 r/min, 30 DEG C
After 24 h cultivated by shaking table, bacterial concentration reaches 108Cfu/mL, is inoculated in propylene hydrochloric acid-propylene by the water absorbent rate of 1:16.85
In amide-type water-retaining agent, it is set to comparison to be inoculated in same amount of culture medium, at 30 DEG C, is incubated at microbiological incubator
In, measure the quantity of Promoting bacteria LX5 in water-retaining agent every 5 days, draw Number dynamics monitoring figure (Fig. 2).It can be seen that
The quantity of tobacco seedlings Promoting bacteria in water-retaining agent in 0-10 days in after falling before raise trend, become on the order of magnitude in 60 days
Change.And in downward trend always in tobacco seedlings Promoting bacteria liquid medium within, after 25 days, in water-retaining agent, LX5 Promoting bacteria quantity is notable
Higher than fluid medium, thus showing, water-retaining agent has the effect that prolongation is grown surely to Promoting bacteria LX5.
Three, water-retaining agent extend tobacco seedlings Promoting bacteria field determine grow experiment
Being inoculated in beef-protein medium by Promoting bacteria LX5,170 rpm/min, 36 h cultivated by 30 DEG C of shaking tables
After, bacterial concentration reaches 107Cfu/mL, is inoculated in propylene hydrochloric acid-acrylic amide water-retaining agent with the water absorbent rate of 1:16.85
In.When tobacco transplant, the water-retaining agent of absorption Promoting bacteria is applied in the soil of transplanting cave, tobacco seedlings is planted on water-retaining agent, water conservation
Agent amount of application is 100 g/ strain cigarettes.From soil, periodically obtain Rhizosphere Soil, measure the Number dynamics change of Promoting bacteria in soil.From
It can be seen that in the mensuration of 90 days, due to the existence of water-retaining agent, the Promoting bacteria in soil is reduced only by one in Fig. 3
The order of magnitude, this result absolutely proves, this water-retaining agent has the effect that prolongation Promoting bacteria is grown in soil surely.
Four, Promoting bacteria water-retaining agent promotes the experiment of cigarette strain growth
By Promoting bacteria in beef-protein medium, 170 r/min, after 36 h cultivated by 30 DEG C of shaking tables, bacterial concentration
Reach 107Cfu/mL, is inoculated in propylene hydrochloric acid-acrylic amide water-retaining agent with the water absorbent rate of 1:16.85.Move Nicotiana tabacum L.
Being applied in the soil of transplanting cave by the water-retaining agent of absorption Promoting bacteria during cultivation, tobacco seedlings planted on water-retaining agent, water-retaining agent amount of application is
100 g/ strain cigarettes.Grow owing to extending Promoting bacteria determining in soil, serve more preferable growth-promoting effect.At Promoting bacteria water-retaining agent
The cigarette strain growing way of reason is considerably better than comparison, and yield increases by 57.28 % (seeing table 1 and Fig. 4).
The table 1 Promoting bacteria water-retaining agent growth-promoting functions to flue-cured tobacco
The above, the only detailed description of the invention of the present invention, but protection scope of the present invention is not limited thereto, and any
The change expected without creative work or replacement, all should contain within protection scope of the present invention.Therefore, the present invention
Protection domain should be as the criterion with the protection domain that claims are limited.
Claims (1)
1. the application in promoting cigarette strain growth jointly of water-retaining agent and tobacco seedlings Promoting bacteria, is first inoculated in water-retaining agent by tobacco seedlings Promoting bacteria
In, apply the most again in the soil of transplanting cave, finally transplanting tobacco seedlings is planted on water-retaining agent；Described tobacco seedlings Promoting bacteria is preserving number
Bacillus licheniformis or its direct transfer thing for CGMCC NO.8266；Described water-retaining agent is propylene hydrochloric acid-acrylic amide
Water-retaining agent.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410115221.3A CN104041521B (en) | 2014-03-26 | 2014-03-26 | The application in field produces of water-retaining agent and Nicotiana tabacum L. Promoting bacteria |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410115221.3A CN104041521B (en) | 2014-03-26 | 2014-03-26 | The application in field produces of water-retaining agent and Nicotiana tabacum L. Promoting bacteria |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN104041521A CN104041521A (en) | 2014-09-17 |
| CN104041521B true CN104041521B (en) | 2017-01-04 |
Family
ID=51495390
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410115221.3A Active CN104041521B (en) | 2014-03-26 | 2014-03-26 | The application in field produces of water-retaining agent and Nicotiana tabacum L. Promoting bacteria |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104041521B (en) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106561231B (en) * | 2016-10-24 | 2020-04-14 | 云南省烟草农业科学研究院 | Method for drought-resistant transplanting of flue-cured tobacco by applying agricultural and forestry water-retaining agent |
| CN115141779B (en) * | 2022-07-26 | 2023-04-07 | 贵州大学 | Growth-promoting bacterium for promoting growth of tobacco seedlings and application thereof |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1276074C (en) * | 2003-11-14 | 2006-09-20 | 神州汉邦（北京)生物技术有限公司 | Lichen bacillus for the production of phytocytomine and plant growth regulator |
| CN101189942A (en) * | 2006-11-29 | 2008-06-04 | 河南农业大学 | Method for preventing and controlling hot pepper epidemic disease |
| CN101054568B (en) * | 2007-04-17 | 2010-05-26 | 周文彩 | Special biological organic fertilizer for tobacco, preparation and use method |
| KR100954297B1 (en) * | 2007-10-05 | 2010-04-20 | 김달수 | Compositions of increasing microbial populations on surfaces and their uses |
-
2014
- 2014-03-26 CN CN201410115221.3A patent/CN104041521B/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| CN104041521A (en) | 2014-09-17 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN109022315A (en) | The biological and ecological methods to prevent plant disease, pests, and erosion bacillus of one plant of broad-spectrum disease resistance and its application | |
| CN101659932A (en) | Antagonistic bacteria preventing and removing continuous cropping tobacco bacterial wilt and microbial organic fertilizer thereof | |
| CN106190917B (en) | The degeneration-resistant production nitrogen pseudomonad of one plant of broad-spectrum disease resistance growth-promoting and its application | |
| CN105331567B (en) | A kind of bacillus amyloliquefaciens and promoting growth of plants preparation and application | |
| CN108624543B (en) | One plant effectively controls eggplant Raul Salmonella and promotes the bacillus amyloliquefaciens of chili growth | |
| CN103484396B (en) | New strain of streptomyces thermocarboxydus and application thereof | |
| CN105838656A (en) | Broad-spectrum disease resisting, growth promotion and stress resisting bacillus capable of preventing and treating tomato gray mold and tomato leaf mold and application of broad-spectrum disease resisting, growth promotion and stress resisting bacillus | |
| CN105567615A (en) | Bradyrhizobium sp. and application thereof | |
| CN107646616A (en) | A kind of preparation method of floriculture substrate | |
| CN104232532B (en) | A kind of plant endogenesis bacillus pumilus and its microbial inoculum, preparation method and application | |
| CN108641989B (en) | Bacillus methylotrophicus and application thereof | |
| CN104480097A (en) | Green alga glycosyl microcapsule biological agent and preparation method and application thereof | |
| CN104177138B (en) | Biological bacterial fertilizer prepared through solid fermentation technology and application of biological bacterial fertilizer | |
| CN110295129A (en) | A kind of biocontrol microorganisms and application for preventing and treating gray mold of cucumber and powdery mildew | |
| CN103497919B (en) | Compound microorganism straw-degrading bacteria agent and preparation method and application thereof | |
| CN101519644B (en) | Sinorhizobium sp. and application thereof | |
| CN105462881A (en) | Paenibacillus polymyxa for preventing and treating vertieillium wilt in crops and application of paenibacillus polymyxa | |
| CN101886055A (en) | Antagonistic bacteria NJL-14 for preventing and controlling continuous-cropping tobacco bacterial wilt | |
| CN103224897A (en) | Bacillussubtilis for tobacco black shank prevention and control | |
| CN106701619B (en) | high-density fermentation method of bacillus amyloliquefaciens and preparation method of microbial inoculum thereof | |
| CN104450573A (en) | Compound ecological microbial agent containing microbial polysaccharide and functional microbes | |
| CN107502565A (en) | One plant of fritillary bulb rhizosphere Paenibacillus polymyxa and its application | |
| CN104152372B (en) | The biocontrol bacterial strain G68 of controlling plant diseases and its bacterial preparation process and application | |
| CN104041521B (en) | The application in field produces of water-retaining agent and Nicotiana tabacum L. Promoting bacteria | |
| CN103650977B (en) | Method for improving survival rate of afforestation of non-woven fabric container seedlings in arid mountains |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| CB03 | Change of inventor or designer information |
Inventor after: Liu Yanxia Inventor after: Li Xiang Inventor after: Shi Junxiong Inventor after: Zhang Heng Inventor before: Liu Yanxia Inventor before: Shi Junxiong Inventor before: Zhang Heng |
|
| COR | Change of bibliographic data | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant |