CN101189942A - Method for preventing and controlling hot pepper epidemic disease - Google Patents
Method for preventing and controlling hot pepper epidemic disease Download PDFInfo
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- CN101189942A CN101189942A CNA2006101282975A CN200610128297A CN101189942A CN 101189942 A CN101189942 A CN 101189942A CN A2006101282975 A CNA2006101282975 A CN A2006101282975A CN 200610128297 A CN200610128297 A CN 200610128297A CN 101189942 A CN101189942 A CN 101189942A
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Abstract
The invention discloses a method of preventing pepper blight, which is that bio-antagonists and solid liquid-preserving agent together is used for treating breeding matrix; the addition weight of the solid liquid-preserving agent is 1 to 2 percent of the breeding matrix; the addition amount of the suspension liquid of the bio-antagonists is 100 to 200ml per 200g of the breeding matrix; the concentration of the suspension liquid of the bio-antagonists is 10<9>cfu/ml. By adopting the method, the survival ability of the bio-antagonists is stable; the colonization density of pepper root region of the bio-antagonists is added during the nursery stage; the pepper blight is prevented and the disease prevention effect in field is improved. In addition, the invention can also enhance seed germination rate, promote seedling growth, and be in favor of seedling growing strong; when being applied to seeding production, the invention can prevent diseases of seedling and improve seedling quality.
Description
Technical field
The invention belongs to plant protection, relate in particular to a kind of method of utilizing biological and ecological methods to prevent plant disease, pests, and erosion microbial control capsicum epidemic disease.
Background technology
Capsicum epidemic disease is a kind of crushing soil-borne disease that is caused by Phytophthora capsici.First since the U.S. finds, now each pepper planting district generally took place this disease in the world from 1918.At present annual because of the capsicum epidemic disease 20%-30% that causes damage, up to 70%-80%, even full field total crop failure is one of severe diseases on capsicum produces when serious.Because of its pathogen time-to-live in soil long, and, endanger more and more serious along with continuous cropping germ number increases year by year.Still lack effective capsicum epidemic disease disease-resistant variety at present on producing, the control of capsicum epidemic disease mainly depends on chemical control, but pathogen develops immunity to drugs to most chemical pesticides, thereby also lacks the effectively preventing measure on producing at present.
In view of this, it is more active to utilize antagonistic microbe to prevent and treat the research of capsicum epidemic disease in recent years.Utilize the biocontrol microorganisms controlling plant diseases, the stability problem of its effect is perplexing the biological control of plant disease always, and the stability of biocontrol effect is subjected to all multifactor influences.To the control of soil-borne disease, the biocontrol microorganisms of introducing is decision biological and ecological methods to prevent plant disease, pests, and erosion success and the key of failing in the power and the stability of root colonization ability.Promptly how to make the biocontrol microorganisms of handling in the soil suppress the key technology that pathogene is the biological and ecological methods to prevent plant disease, pests, and erosion success at rhizosphere soil and the certain density of plant root long term maintenance.
Summary of the invention
The purpose of this invention is to provide a kind of method of preventing and treating capsicum epidemic disease, decide to grow density, control sprout term disease, and improve the protection effect in field at the capsicum rhizosphere by stabilizing and increasing biocontrol microorganisms.
For achieving the above object, the present invention adopts following technical scheme: a kind of method of preventing and treating capsicum epidemic disease, with biocontrol microorganisms and solid water-loss reducer joining process in seedling medium, the addition of solid water-loss reducer is 1%～2% of a seedling medium weight, the addition of biocontrol microorganisms suspension is that every 200g seedling medium adds 100～200ml, and the concentration of biocontrol microorganisms suspension is 10
9Cfu/ml.
Described biocontrol microorganisms is Serratia plymuthica (Serratia plymuthica).
The Serratia plymuthica of adopting among the present invention belongs to the blue formula negative bacterium of leather, and thalline is shaft-like, 0.6-0.7 * 1.4-2.5 micron; The bacterium colony circle, neat in edge, smooth surface, glossy; Optimum growth temperature is 28-30 ℃, and optimum pH value is 6.5-8.0, generally cultivates on protein culture medium.Can strong inhibition the mycelial growth of phytophthora capsici, suppress the formation of zoosporangium and zoospore, suppress zoosporangium and by the germination of cystospore; Suppress the concentration of soil root circle phytophthora capsici and prevent the pathogen intrusion; Produce chlorinated macrolide series antibiotics macrocyclic lactone A21-4.
The solid water-loss reducer is that the super absorbent polymer compound also is solid water (Dry Water), can stablize the survival ability of biocontrol microorganisms in matrix with biocontrol microorganisms and solid water-loss reducer joining process, and nursery stage increases biocontrol microorganisms and decides to grow density at the capsicum rhizosphere.
In seedling medium, abbreviate PS-DW-BA with biocontrol microorganisms and solid water-loss reducer joining process as, have the following advantages:
1, as shown in Figure 1, can improve the survival density of biocontrol microorganisms in matrix: biocontrol microorganisms and solid water-loss reducer joining process in seedling medium than biocontrol microorganisms independent the processing in seedling medium improve the survival rate of biocontrol microorganisms more than 10 times;
2, as shown in Figure 2, utilize PS-DW-BA matrix to educate the capsicum seedling, raising biocontrol microorganisms deciding in capsicum root and rhizosphere soil grown density and respectively improved 100 times and 10 times;
3, shown in Fig. 3, table 1, in pot experiment, the capsicum seedling that utilizes PS-DW-BA matrix to educate, control capsicum epidemic disease effect reaches 100%.
The capsicum epidemic disease incidence of disease of the capsicum seedling that the seedling medium with biocontrol microorganisms and solid water-loss reducer joining process that table 1. utilizes biocontrol microorganisms to handle is separately educated
a0.05 significant difference on the level.
4, capsicum seedling as shown in table 2, as to utilize PS-DW-BA matrix to educate is transplanted to its control efficiency of land for growing field crops and is reached 70%, improves output more than 20%;
The capsicum epidemic disease incidence of disease and yield of hot pepper were relatively when the capsicum field was arrived in the capsicum transplantation of seedlings that the seedling medium with biocontrol microorganisms and solid water-loss reducer joining process that table 2. utilizes biocontrol microorganisms to handle is separately educated
a0.05 significant difference on the level.
bYield of hot pepper is the output of accumulation.
5, as shown in Figure 4, utilize PS-DW-BA matrix to educate the capsicum seedling, improve percentage of seedgermination about 40%;
6, as shown in table 3, utilize PS-DW-BA matrix to educate the capsicum seedling, promote growth of seedling, improve seedling quality, increase the benefit.
The seedling medium of table 3. biocontrol microorganisms and solid water-loss reducer joining process promotes capsicum seedling growth result
a0.05 significant difference on the level.
In addition, pour into processing once more during the capsicum transplantation of seedlings after soaking the root processing in the biocontrol microorganisms suspension, transplanting and can control capsicum epidemic disease effectively, it reaches 76% in protection ground cultural control effect.
Description of drawings
Fig. 1 is the survival density comparison diagram of biocontrol microorganisms in biocontrol microorganisms seedling medium of handling separately and the seedling medium of using biocontrol microorganisms and solid water-loss reducer joining process.
Fig. 2 be when utilizing seedling medium that biocontrol microorganisms handles separately and educating the capsicum seedling with the seedling medium of biocontrol microorganisms and solid water-loss reducer joining process the biocontrol microorganisms in capsicum root and the rhizosphere soil decide to grow the density comparison diagram.
Fig. 3 is the capsicum epidemic disease occurring degree comparison diagram that utilizes biocontrol microorganisms seedling medium of handling separately and the capsicum seedling of educating with the seedling medium of biocontrol microorganisms and solid water-loss reducer joining process.
Fig. 4 is the percentage of seedgermination comparison diagram of capsicum in the seedling medium of biocontrol microorganisms and solid water-loss reducer joining process.
Embodiment
Embodiment 1: in the present embodiment, select Sinangro Co.Jinju for use, and the seedling medium that Korea produces, JRM chemical.Inc.Cleveland, Ohio, the solid water-loss reducer that USA produces, Serratia plymuthica is selected KACC91013 for use.
Get dry seedling medium double centner, evenly mix with 1 kilogram of solid water-loss reducer, the reason of selecting dry seedling medium for use is that the suction of solid water-loss reducer is congealed into one when mixing.Be 10 with concentration then
9100 liters of the Serratia plymuthica suspension of cfu/ml join in the mixture of above-mentioned matrix and solid water-loss reducer, and the limit adds stirring, and it is mixed, can be in order to grow seedlings.
Embodiment 2: in the present embodiment, select the seedling medium double centner for use, and 2 kilograms of solid water-loss reducers, concentration is 10
9100 liters of the Serratia plymuthica suspension of cfu/ml, other is identical with embodiment 1.
Embodiment 3: in the present embodiment, select 1000 kilograms of seedling mediums for use, and 20 kilograms of solid water-loss reducers, concentration is 10
9750 liters of the Serratia plymuthica suspension of cfu/ml, other is identical with embodiment 1.
Embodiment 4: in the present embodiment, select 200 kilograms of seedling mediums for use, and 3 kilograms of solid water-loss reducers, concentration is 10
9100 liters of the Serratia plymuthica suspension of cfu/ml, other is identical with embodiment 1.
Embodiment 5: in the present embodiment, select the seedling medium double centner for use, and 1.2 kilograms of solid water-loss reducers, concentration is 10
990 liters of the Serratia plymuthica suspension of cfu/ml, other is identical with embodiment 1.
Embodiment 7: in the present embodiment, select 1000 kilograms of seedling mediums for use, and 19 kilograms of solid water-loss reducers, concentration is 10
9850 liters of the Serratia plymuthica suspension of cfu/ml, other is identical with embodiment 1.
Embodiment 8: in the present embodiment, select the seedling medium double centner for use, and 1 kilogram of solid water-loss reducer, concentration is 10
950 liters of the Serratia plymuthica suspension of cfu/ml, other is identical with embodiment 1.
Embodiment 9: in the present embodiment, select the seedling medium double centner for use, and 1.7 kilograms of solid water-loss reducers, concentration is 10
960 liters of the Serratia plymuthica suspension of cfu/ml, other is identical with embodiment 1.
Embodiment 10: in the present embodiment, select the seedling medium double centner for use, and 1.5 kilograms of solid water-loss reducers, concentration is 10
975 liters of the Serratia plymuthica suspension of cfu/ml, other is identical with embodiment 1.
Embodiment 11: in the present embodiment, select 10 kilograms of seedling mediums for use, and solid water-loss reducer 130 grams, concentration is 10
98 liters of the Serratia plymuthica suspension of cfu/ml, other is identical with embodiment 1.
Claims (2)
1. method of preventing and treating capsicum epidemic disease, it is characterized in that: use biocontrol microorganisms and solid water-loss reducer joining process in seedling medium, the addition of solid water-loss reducer is 1%～2% of a seedling medium weight, the addition of biocontrol microorganisms suspension is that every 100g seedling medium adds 50～100ml, and the concentration of biocontrol microorganisms suspension is 10
9Cfu/ml.
2. the method for control capsicum epidemic disease as claimed in claim 1 is characterized in that: described biocontrol microorganisms is Serratia plymuthica (Serratia plymuthica).
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| CNA2006101282975A CN101189942A (en) | 2006-11-29 | 2006-11-29 | Method for preventing and controlling hot pepper epidemic disease |
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| CNA2006101282975A CN101189942A (en) | 2006-11-29 | 2006-11-29 | Method for preventing and controlling hot pepper epidemic disease |
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Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102154178A (en) * | 2011-01-28 | 2011-08-17 | 广东省农业科学院植物保护研究所 | Bacillus brevis for preventing and treating hot pepper epidemic disease as well as preparation method and application of biological agent |
| CN102939848A (en) * | 2012-11-30 | 2013-02-27 | 赖月娥 | Pest control and management method in seedling period of peppers |
| CN103122330A (en) * | 2012-12-31 | 2013-05-29 | 陈秀蓉 | Alpine grassland pasture endogenous serratia plymuthica strain GH010 and application thereof as well as microbial agent prepared from same and preparation method |
| CN103415613A (en) * | 2011-01-11 | 2013-11-27 | 基础服务农业研究院 | Serratia plymuthica for biological control of bacterial plant pathogens |
| CN103931658A (en) * | 2014-04-28 | 2014-07-23 | 贵州省烟草科学研究院 | Application of water-retraining agent and ralstonia solannacearum antagonistic bacteria to field production |
| CN104041521A (en) * | 2014-03-26 | 2014-09-17 | 贵州省烟草科学研究院 | Use of water-retaining agent and tobacco growth-promoting bacteria in production in field |
| CN104302758A (en) * | 2012-05-16 | 2015-01-21 | 罗盖特兄弟公司 | Strain producing turanose and uses thereof |
| CN105409557A (en) * | 2015-12-09 | 2016-03-23 | 福建省农业科学院植物保护研究所 | Method for inducing disease resistance of pepper system by taking phosphite as exciton |
| CN105766582A (en) * | 2016-03-23 | 2016-07-20 | 山东圣翔节能科技有限公司 | Functional seedling culturing matrix |
| CN110144301A (en) * | 2018-02-10 | 2019-08-20 | 河南农业大学 | A kind of Pseudomonas chlororaphis and its cultural method and application |
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2006
- 2006-11-29 CN CNA2006101282975A patent/CN101189942A/en active Pending
Cited By (17)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103415613A (en) * | 2011-01-11 | 2013-11-27 | 基础服务农业研究院 | Serratia plymuthica for biological control of bacterial plant pathogens |
| CN102154178B (en) * | 2011-01-28 | 2012-09-19 | 广东省农业科学院植物保护研究所 | Bacillus brevis for preventing and treating hot pepper epidemic disease as well as preparation method and application of biological agent |
| CN102154178A (en) * | 2011-01-28 | 2011-08-17 | 广东省农业科学院植物保护研究所 | Bacillus brevis for preventing and treating hot pepper epidemic disease as well as preparation method and application of biological agent |
| CN104302758A (en) * | 2012-05-16 | 2015-01-21 | 罗盖特兄弟公司 | Strain producing turanose and uses thereof |
| CN104302758B (en) * | 2012-05-16 | 2016-10-05 | 罗盖特兄弟公司 | Produce turanose bacterial strain and application thereof |
| CN102939848A (en) * | 2012-11-30 | 2013-02-27 | 赖月娥 | Pest control and management method in seedling period of peppers |
| CN103122330B (en) * | 2012-12-31 | 2015-04-08 | 甘肃农业大学 | Alpine grassland pasture endogenous serratia plymuthica strain GH010 and application thereof as well as microbial agent prepared from same and preparation method |
| CN103122330A (en) * | 2012-12-31 | 2013-05-29 | 陈秀蓉 | Alpine grassland pasture endogenous serratia plymuthica strain GH010 and application thereof as well as microbial agent prepared from same and preparation method |
| CN104041521A (en) * | 2014-03-26 | 2014-09-17 | 贵州省烟草科学研究院 | Use of water-retaining agent and tobacco growth-promoting bacteria in production in field |
| CN103931658A (en) * | 2014-04-28 | 2014-07-23 | 贵州省烟草科学研究院 | Application of water-retraining agent and ralstonia solannacearum antagonistic bacteria to field production |
| CN103931658B (en) * | 2014-04-28 | 2017-01-18 | 贵州省烟草科学研究院 | Application of water-retraining agent and ralstonia solannacearum antagonistic bacteria to field production |
| CN105409557A (en) * | 2015-12-09 | 2016-03-23 | 福建省农业科学院植物保护研究所 | Method for inducing disease resistance of pepper system by taking phosphite as exciton |
| CN105409557B (en) * | 2015-12-09 | 2018-03-23 | 福建省农业科学院植物保护研究所 | The method that capsicum systemic disease resistance is induced using phosphite as exciton |
| CN105766582A (en) * | 2016-03-23 | 2016-07-20 | 山东圣翔节能科技有限公司 | Functional seedling culturing matrix |
| CN105766582B (en) * | 2016-03-23 | 2020-03-27 | 山东圣翔节能科技有限公司 | Functional seedling culture substrate |
| CN110144301A (en) * | 2018-02-10 | 2019-08-20 | 河南农业大学 | A kind of Pseudomonas chlororaphis and its cultural method and application |
| CN110144301B (en) * | 2018-02-10 | 2022-04-01 | 河南农业大学 | Pseudomonas chlororaphis and culture method and application thereof |
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Open date: 20080604 |