CN105409557A - Method for inducing disease resistance of pepper system by taking phosphite as exciton - Google Patents
Method for inducing disease resistance of pepper system by taking phosphite as exciton Download PDFInfo
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- CN105409557A CN105409557A CN201510902052.2A CN201510902052A CN105409557A CN 105409557 A CN105409557 A CN 105409557A CN 201510902052 A CN201510902052 A CN 201510902052A CN 105409557 A CN105409557 A CN 105409557A
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- phosphite
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
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Abstract
The present invention relates to a method for inducing disease resistance of a pepper system by taking phosphite as an exciton and an application. The method comprises the following steps: firstly, preparing the phosphite; secondly, producing pepper seedlings; and finally, detecting disease resistance defense reaction performance of a phosphite exciton induced pepper, treating the cultured pepper seedlings with the prepared phosphite in a spraying method, performing inducing treatment for 48 hours, then inoculating the etiolated seedlings with Phytophthora capsici Zoospore suspension liquid, and observing morbidity of the seedlings after 3-5 days to determine the effect of the liquid to induce plant disease resistance. By the method, the phosphite adapted to the field application can be prepared in a time and labor saving manner, meanwhile, disease resistance defense reaction activation is detected, so that the gap of understanding in studying disease resistance of pepper by using phosphite as the exciton to activate defense responses and to enhance disease resistance is filled up, and the method has a great significance.
Description
Technical field
The present invention relates to technical field of plant protection, disclose the method for inducing capsicum systemic disease resistance using phosphite as exciton.
Background technology
Capsicum epidemic disease is the crushing fungal disease generally occurred in a kind of world wide, and first it found in 1918 in New Mexico, causes the phytophthora blight of pepper of capsicum epidemic disease
phytophthoracapsicileonian is that LeonLeonian named in nineteen twenty-two, and it belongs to pathogenic oomycetes.Phytophthora capsici is survived the winter in soil invalid body with egg spore or chlamydospore, and can survive even longer time several months, host range is wide, mainly infects the multiple important vegetable such as capsicum, tomato, eggplant, cucumber, pumpkin.Capsicum epidemic disease open country and protecting field all can occur, and the popular of this disease causes capsicum to lose seriously, and general diseased plant rate is 15 – 30%, reaches more than 80% time serious.Since nineteen eighty, capsicum epidemic disease increases the weight of increasingly at China's growing vegetables region occurring degree, has had a strong impact on the economic benefit of growing vegetables industry, produces cause huge economic loss to the vegetable safety of China.Therefore, phytophthora blight of pepper is subject to the height extensive concern of various countries phytopathologist and breeding scholar.After the chemical substances such as discovered in recent years salicylic acid are absorbed by plant corpus, the disease resistance of host plant can be strengthened, reduce the generation of disease.
Phosphite has been developed to the field control for multiple diseases of agricultural chemicals or fertilizer, but its real diseases prevention Mechanism Study is not also understood.By to the research utilizing phosphite to activate the understanding of pepper disease resistance defense response, can understand phosphite in depth to the diseases prevention of phytophthora blight of pepper mechanism searches out disease-resistant gene relevant to this diseases prevention mechanism in capsicum as target, and above-mentioned research is for understanding fully that the mechanism of action of phosphite in pepper disease resistance defense response provides basic.By resolving the molecular mechanism of the pepper disease resistance defense response that phosphite activates, can find new resistance locus for breeding expert, the final outburst for effectively controlling epidemic disease proposes new strategy.But current research shows, the disappearance that the molecular mechanism of phosphite in pepper disease resistance defense response is resolved limits our further investigation to its mechanisms of control wheat scab.
Summary of the invention
The object of the invention is to provide the method for inducing capsicum systemic disease resistance using phosphite as exciton, being namely experiment material with Hot Pepper Seedling, detecting inducing anti-disease effect by phosphite as inoculating phytophthora blight of pepper after exciton process.
Of the present invention utilize phosphite to activate pepper disease resistance defense response method and applying step as follows:
(1) the preparation of phosphite is heavily used with 1:1 etc. purity > 95% phosphorous acid and potassium hydroxide, first with water-soluble solution phosphorous acid 1g, dissolved hydrogen potassium oxide 1g on its basis again, by above-mentioned solution constant volume to 1000mL, the pH value of deployed phosphite is about 6.2, and adding the Tween-20 accounting for total volume fraction 2 ‰ can directly use;
(2) pepper seed is after 30% clorox (Shanghai traditional Chinese medicines Cas#:7681-52-9) surface sterilization through volume fraction, rinse well with sterile purified water again, scald in 55 DEG C ~ 58 DEG C water-baths and plant after 20 minutes, by planting seed (30*50cm) in the enamel tray of the two-layer gauze of underlay, then in 28 DEG C of incubators, 5-7 days is cultivated under dark lucifuge condition, until grow elongated capsicum etiolated seedling, shift out illumination box and cultivate 2-3 days;
(3) the detection method of phosphite exciton induction pepper disease resistance defense response usefulness is, first, by the phosphite for preparing with the above-mentioned cultured Hot Pepper Seedling of spray-on process process, induction process is after 48 hours, then stop phytophthora blight of pepper zoospore liquid spray inoculation, incidence is observed, to determine its effect to inducing plant disease resistance after 3-5 days.
The stop preparation method of liquid of phytophthora blight of pepper zoospore is inoculated on V8 flat board for getting 0.3cm × 0.3cm-1cm × 1cm phytophthora blight of pepper 4 pieces, 25 DEG C of growths produce a large amount of sporangium in 10 days, 15min is placed by above-mentioned dull and stereotyped 4 DEG C, then namely obtain phytophthora blight of pepper zoospore after 30min at being placed in 25 DEG C, vortex oscillation is the liquid that stops in 30 seconds.
V8 flat board is 10%V8 culture fluid: 100mLV8 vegetable juice adds 0.2gCaCO
3after, centrifugal 5min under 3000 revs/min, gets supernatant constant volume to 1000ml, adds the agar powder of cumulative volume 1.5%.
The invention solves the problem utilizing ite to activate pepper disease resistance defense response, be the effect that examination material detects phosphorous acid inducing anti-disease defense response with above-mentioned Hot Pepper Seedling, have experimental period short, do not affect by external environmental condition, experimental repeatability is good, inoculation Symptoms is obvious, be easy to the advantage such as classification and Germicidal efficacy.By resolving the molecular mechanism of the pepper disease resistance defense response that phosphite activates, can find new resistance locus for breeding expert, the final outburst for effectively controlling epidemic disease proposes new strategy.But current research shows, the disappearance that the extension set mechanism of phosphite in pepper disease resistance defense response is resolved limits our further investigation to its mechanisms of control wheat scab.
Embodiment
According to phosphorous acid 1g: the weight ratio of potassium hydroxide 1g prepares phosphite solution 1000mL, pepper seed induction after sterilization produces Hot Pepper Seedling, finally detects phosphite exciton induction pepper disease resistance defense response usefulness.
embodiment 1:
According to phosphorous acid 1g: the weight ratio preparation phosphite solution of potassium hydroxide 1g, first with water-soluble solution phosphorous acid 1g, dissolved hydrogen potassium oxide 1g on its basis again, by above-mentioned solution constant volume to 1000mL, the pH value of deployed phosphite is about 6.2, add account for total volume fraction be 2 ‰ Tween-20 can directly use; Platform green pepper 106 and U.S.'s Christian era green pepper seed are after clorox (the Shanghai traditional Chinese medicines Cas#:7681-52-9) surface sterilization of 30% volume, rinse well with sterile purified water again, scald in 55 DEG C of water-baths and plant after 20 minutes, by planting seed (30*50cm) in the enamel tray of the two-layer gauze of underlay, then cultivate 5 days under dark lucifuge condition in 28 DEG C of incubators, until grow elongated capsicum etiolated seedling, shift out illumination box and cultivate 2 days; Phosphorous acid as the detection method of exciton induction pepper disease resistance defense response usefulness is, first, by the phosphite for preparing with the above-mentioned cultured Hot Pepper Seedling of spray-on process process, induction process is after 48 hours, then to stop liquid (10 with phytophthora blight of pepper zoospore
7individual/mL) spray inoculation Hot Pepper Seedling, within 3 days, observe seedling incidence afterwards, to determine its effect to inducing plant disease resistance, simultaneously to spray clear water in contrast.
The stop preparation method of liquid of phytophthora blight of pepper zoospore is inoculated on V8 flat board for getting 1cm × 1cm phytophthora blight of pepper 4 pieces, 25 DEG C of growths produce a large amount of sporangium in 10 days, 15min is placed by above-mentioned dull and stereotyped 4 DEG C, then namely obtain phytophthora blight of pepper zoospore after 30min at being placed in 25 DEG C, vortex oscillation is the liquid that stops in 30 seconds.
V8 flat board is 10%V8 culture fluid: 100mLV8 vegetable juice adds 0.2gCaCO
3after, centrifugal 5min under 3000 revs/min, gets supernatant constant volume to 1000ml, adds the agar powder of 1.5wt.%.
Experimental result statistical analysis finds that the effect of phosphite inducing anti-disease is 75.67%.
Embodiment 2
According to phosphorous acid 1g: the weight ratio preparation phosphorous acid solution of potassium hydroxide 1g, first with water-soluble solution phosphorous acid 1g, dissolved hydrogen potassium oxide 1g on its basis again, by above-mentioned solution constant volume to 1000mL, the pH value of deployed phosphite is 6.2, add account for total volume fraction be 2 ‰ Tween-20 can directly use; Red sun and spring peppery No. 19 seeds are after 30% clorox surface sterilization, rinse well with sterile purified water again, scald in 58 DEG C of water-baths and plant after 20 minutes, by planting seed (30*50cm) in the enamel tray of the two-layer gauze of underlay, then cultivate 7 days under dark lucifuge condition in 28 DEG C of incubators, until grow elongated capsicum etiolated seedling, shift out illumination box and cultivate 3 days; The detection method of phosphorous acid exciton induction pepper disease resistance defense response usefulness is, first, by the phosphite for preparing with the above-mentioned cultured Hot Pepper Seedling of spray-on process process, induction process is after 48 hours, then to stop liquid (10 with phytophthora blight of pepper zoospore
7individual/mL) inoculate etiolated seedling, within 4 days, observe seedling incidence afterwards, to determine its effect to inducing plant disease resistance, simultaneously to spray clear water in contrast.
The stop preparation method of liquid of phytophthora blight of pepper zoospore is inoculated on V8 flat board for getting 0.3cm × 0.3cm phytophthora blight of pepper 4 pieces, 25 DEG C of growths produce a large amount of sporangium in 10 days, 15min is placed by above-mentioned dull and stereotyped 4 DEG C, then namely obtain phytophthora blight of pepper zoospore after 30min at being placed in 25 DEG C, vortex oscillation is the liquid that stops in 30 seconds.
V8 flat board is
10%V8 culture fluid: 100mLV8 vegetable juice adds 0.2gCaCO
3after, centrifugal 5min under 3000 revs/min, gets supernatant constant volume to 1000ml, adds the agar powder of cumulative volume 1.5wt.%.
Experimental result statistical analysis finds that the effect of phosphite inducing anti-disease is 78.53%.
The above results shows, by utilizing phosphite to activate the defense responses of capsicum as exciton, can significantly improve the resistance against diseases of Hot Pepper Seedling, and this is significant to the anti-disease mechanism research and resistive breeding research that promote capsicum.
The foregoing is only preferred embodiment of the present invention, all equalizations done according to the present patent application the scope of the claims change and modify, and all should belong to covering scope of the present invention.
Claims (3)
1. induce the method for capsicum systemic disease resistance using phosphite as exciton, it is characterized in that: following several respects:
(1) the preparation of ite is heavily used with 1:1 etc. purity > 95% phosphorous acid and potassium hydroxide, first with water-soluble solution phosphorous acid 1g, dissolved hydrogen potassium oxide 1g on its basis again, by above-mentioned solution constant volume to 1000mL, the pH value of deployed phosphite is 6.2, add the Tween-20 mixing accounting for cumulative volume 2 ‰, stand-by;
(2) pepper seed is after 30% clorox surface sterilization through volume fraction, rinse well with sterile purified water again, scald in 55 DEG C ~ 58 DEG C water-baths and plant after 20 minutes, by planting seed in the enamel tray at the two-layer gauze of soil underlay, then in 28 DEG C of incubators, 5-7 days is cultivated under dark lucifuge condition, until grow elongated capsicum etiolated seedling, shift out illumination box and cultivate 2-3 days;
(3) phosphite step (1) prepared is with the above-mentioned cultured Hot Pepper Seedling of spray-on process process, induction process is after 48 hours, then to stop liquid spray inoculation with phytophthora blight of pepper zoospore, after 3-5 days, observe incidence, to determine its effect to inducing plant disease resistance.
2. method of inducing capsicum systemic disease resistance using phosphite as exciton according to claim 1, it is characterized in that: the stop preparation method of liquid of phytophthora blight of pepper zoospore is inoculated on V8 flat board for getting 0.3cm × 0.3cm-1cm × 1cm phytophthora blight of pepper 4 pieces, 25 DEG C of growths produce a large amount of sporangium in 10 days, 15min is placed by above-mentioned dull and stereotyped 4 DEG C, then namely obtain phytophthora blight of pepper zoospore after 30min at being placed in 25 DEG C, vortex oscillation is the liquid that stops in 30 seconds.
3. method of inducing capsicum systemic disease resistance using phosphite as exciton according to claim 2, is characterized in that: V8 flat board is 10%V8 culture fluid: 100mLV8 vegetable juice adds 0.2gCaCO
3after, centrifugal 5min under 3000 revs/min, gets supernatant constant volume to 1000ml, adds the agar powder of cumulative volume 1.5%.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN106613555A (en) * | 2016-12-29 | 2017-05-10 | 福建省农业科学院植物保护研究所 | Method for inducing pepper against pestilence by using benzothiadiazole as exciton |
| CN110089326A (en) * | 2019-03-08 | 2019-08-06 | 贵州民族大学 | A kind of adjuvant and the method using its raising masson pine disease-resistant performance |
| CN110564667A (en) * | 2019-09-25 | 2019-12-13 | 云南省烟草农业科学研究院 | method for efficiently suspending and concentrating zoospores of plant pathogenic oomycetes and application of zoospores |
| CN110896842A (en) * | 2019-12-27 | 2020-03-24 | 华中农业大学 | Efficient cultivation method of citrus virus-free etiolation stock and application thereof |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106613555A (en) * | 2016-12-29 | 2017-05-10 | 福建省农业科学院植物保护研究所 | Method for inducing pepper against pestilence by using benzothiadiazole as exciton |
| CN110089326A (en) * | 2019-03-08 | 2019-08-06 | 贵州民族大学 | A kind of adjuvant and the method using its raising masson pine disease-resistant performance |
| CN110564667A (en) * | 2019-09-25 | 2019-12-13 | 云南省烟草农业科学研究院 | method for efficiently suspending and concentrating zoospores of plant pathogenic oomycetes and application of zoospores |
| CN110564667B (en) * | 2019-09-25 | 2023-02-28 | 云南省烟草农业科学研究院 | Method for efficiently suspending and concentrating zoospores of plant pathogenic oomycetes and application of zoospores |
| CN110896842A (en) * | 2019-12-27 | 2020-03-24 | 华中农业大学 | Efficient cultivation method of citrus virus-free etiolation stock and application thereof |
| CN110896842B (en) * | 2019-12-27 | 2021-07-30 | 华中农业大学 | Efficient cultivation method of citrus virus-free etiolation stock and application thereof |
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