CN104004660A - Shake-flask spawn culture composition of cordyceps militaris - Google Patents
Shake-flask spawn culture composition of cordyceps militaris Download PDFInfo
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- CN104004660A CN104004660A CN201410186454.2A CN201410186454A CN104004660A CN 104004660 A CN104004660 A CN 104004660A CN 201410186454 A CN201410186454 A CN 201410186454A CN 104004660 A CN104004660 A CN 104004660A
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Abstract
A shake-flask spawn culture composition of cordyceps militaris relates to the technical field of culture of cordyceps militaris, and is prepared from the following raw materials in parts by weight: 100-150 parts of tomato powder, 15-20 parts of glucose, 3-5 parts of peptone, 1.5-1.8 parts of potassium dihydrogen phosphate, 1.2-1.5 parts of magnesium sulfate, 1000-1200 parts of water, 15-30 parts of corn flour, 10-15 parts of maltose, 2-5 parts of egg white, 3-8 parts of pupa slurry and 0.5-1.5 parts of vegetable juice. The cordyceps militaris medium is capable of substantially improving the content of cordycepin and cordyceps acid in cordyceps militaris, enabling cordyceps militaris to have stronger pharmaceutical functions and health-care function, and promoting growth and breeding of cordyceps militaris florae.
Description
Technical field
The present invention relates to Cordyceps militaris (L.) Link. culture technique field, relate in particular to a kind of Cordyceps militaris (L.) Link. shaking flask spawn culture composition.
Background technology
The worldwide distribution natural resource of Cordyceps militaris (L.) Link. quantity is little.By stroma (being careless part) and sclerotium (being the corpse part of worm) complex body dimerous.Winter, larva lived in seclusion in soil, and mushroom parasitism wherein, is drawn nutrition, was full of mycelia and dead in larva body.To summer, on larva corpse, bear seedling, likeness in form grass, front and back gathered and obtained the Summer Solstice.Cordyceps militaris (L.) Link. has edible and pharmaceutical use.
The domestic research to cordyceps militaris plantation and substratum is at present more traditional.Cause cordycepin content in Cordyceps militaris (L.) Link. generally in 4000mg/kg left and right, cordycepic acid content is only in 12% left and right.And cordycepin and the cordycepic acid part of most critical in the nutritive substance of Cordyceps militaris (L.) Link. just.Cordycepin is nucleoside antibiotics, and cordycepic acid is a kind of D type N.F,USP MANNITOL.Improve the content of these two kinds of materials in Cordyceps militaris (L.) Link., can greatly promote Cordyceps militaris (L.) Link. to insomnia, rheumatism, rheumatism, cardiovascular disease alleviation and auxiliary therapeutic action.
Summary of the invention
Technical problem to be solved by this invention is to overcome the defect of prior art, further proposes a kind of Cordyceps militaris (L.) Link. shaking flask spawn culture composition.
Technical problem to be solved by this invention realizes by the following technical solutions.
A kind of Cordyceps militaris (L.) Link. shaking flask spawn culture composition, is characterized in that being made up of the material of following former weight part: 100～150 parts of mealy potatos, 15～20 parts of glucose, 3～5 parts of peptones, 1.5～1.8 parts of potassium primary phosphates, 1.2～1.5 parts, magnesium sulfate, 1000～1200 parts, water, 15～30 parts of Semen Maydis powder, 10～15 parts of maltose, 2～5 parts, egg white, 3～8 parts, pupa slurry, 0.5～1.5 part, green vegetables juice;
Preparation method is:
After being mixed in proportion, each raw material of group component prepares Cordyceps militaris kind substratum, Cordyceps militaris kind substratum packs in shaking flask, every bottled enter liquid measure account for a bottle cubic capacity 40-45%, sealing sterilizing, after cooling, under aseptic condition, access Cordyceps militaris (L.) Link. slant strains, the artificial intermittently shake-flask culture 5-10 days that first leaves standstill 24-36 hour again at 22～25 DEG C, more than 3 hours observe cotton-shaped mycelia natural packing body and account for whole liquid volume time over half wait leaving standstill, obtain Cordyceps militaris bacterial classification and can be directly used in cordyceps militaris plantation through rejecting the sample of disqualified upon inspection.
Further preferred version,
The feed composition also comprising in composition is expanded bean powder, expanded bean powder and Semen Maydis powder equivalent in component.
The preparation method of described expanded bean powder is: first soybean is soaked in water 2～5 hours, pulls draining in the time that fold appears in soybean epidermis out, grind to form bean powder after draining, make bean powder surface air-dry, and then bean powder is added and in extruser, carries out puffing.Bean powder is after puffing, and nutritive substance is more easily utilized by mushroom.
The pH value of the water in Chinese caterpillar fungus culture medium of the present invention is 7.4, and in water, contains the zinc of 0.03～0.1mg/L, free carbon dioxide, the calcium of 60～70mg/L and the iodide of 0.1～0.2mg/L of 10～12mg/L.
Beneficial effect of the present invention is:
The present invention is fully studying growth characteristic and the bacterial classification habit of Cordyceps militaris (L.) Link. under physical environment, and selected a kind of high quality combination formula.The present invention adopts maltose and glucose acting in conjunction, and Cordyceps militaris (L.) Link. mushroom, due to the Growth and reproduction of hobby sugar energy strong impulse Cordyceps militaris (L.) Link. flora, improves the Cordyceps polysaccharide content in Cordyceps militaris (L.) Link. greatly; Glucose can directly be utilized by mushroom, and maltose can be decomposed utilization for a long time by maltose.
Green vegetables juice add the Growth and reproduction that can promote Cordyceps militaris (L.) Link. flora, for Cordyceps militaris (L.) Link. fungus group growth and breeding provides necessary vegetable-protein and trace element.
The feature of the Cordyceps militaris (L.) Link. of cultivating according to Chinese caterpillar fungus culture medium of the present invention is: surviving rate reaches 99%, and more than cordycepin content reaches 8500mg/kg, cordycepic acid content exceedes 18%.
Of the present invention another more remarkable with being worth.Its dominant mechanism is: 1, the composition of egg white and human protein composition are the most approaching.2, this mixed small peptide being made up of 2-9 amino acid has unique amino acid structure and physiological function.
Chinese caterpillar fungus culture medium of the present invention can improve cordycepin in Cordyceps militaris (L.) Link. and the content of cordycepic acid greatly, makes its medicinal function and nourishing function stronger.
Embodiment
For technique means, creation characteristic that the present invention is realized, reach object and effect is easy to understand, below in conjunction with specific embodiment, further set forth the present invention.
Embodiment 1
A kind of Cordyceps militaris (L.) Link. shaking flask spawn culture composition, is made up of the material of following former weight: mealy potato 100g, glucose 15g, peptone 3g, potassium primary phosphate 1.5g, magnesium sulfate 1.2g, water 1000g, Semen Maydis powder 15g, maltose 10g, egg white 2g, pupa slurry 3g, green vegetables juice 0.5g;
Preparation method is:
After being mixed in proportion, each raw material of group component prepares Cordyceps militaris kind substratum, Cordyceps militaris kind substratum packs in shaking flask, every bottled enter liquid measure account for a bottle cubic capacity 40-45%, sealing sterilizing, after cooling, under aseptic condition, access Cordyceps militaris (L.) Link. slant strains, the artificial intermittently shake-flask culture 5-10 days that first leaves standstill 24-36 hour again at 22～25 DEG C, more than 3 hours observe cotton-shaped mycelia natural packing body and account for whole liquid volume time over half wait leaving standstill, obtain Cordyceps militaris bacterial classification and can be directly used in cordyceps militaris plantation through rejecting the sample of disqualified upon inspection.
Embodiment 2
A kind of Cordyceps militaris (L.) Link. shaking flask spawn culture composition, is made up of the material of following former weight: mealy potato 150g, glucose 20g, peptone 5g, potassium primary phosphate 1.8g, magnesium sulfate 1.5g, water 1200g, Semen Maydis powder 30g, maltose 15g, egg white 5g, pupa slurry 8g, green vegetables juice 1.5g;
Preparation method is:
After being mixed in proportion, each raw material of group component prepares Cordyceps militaris kind substratum, Cordyceps militaris kind substratum packs in shaking flask, every bottled enter liquid measure account for a bottle cubic capacity 40-45%, sealing sterilizing, after cooling, under aseptic condition, access Cordyceps militaris (L.) Link. slant strains, the artificial intermittently shake-flask culture 5-10 days that first leaves standstill 24-36 hour again at 22～25 DEG C, more than 3 hours observe cotton-shaped mycelia natural packing body and account for whole liquid volume time over half wait leaving standstill, obtain Cordyceps militaris bacterial classification and can be directly used in cordyceps militaris plantation through rejecting the sample of disqualified upon inspection.
Embodiment 3
A kind of Cordyceps militaris (L.) Link. shaking flask spawn culture composition, is made up of the material of following former weight: mealy potato 125g, glucose 18g, peptone 4g, potassium primary phosphate 1.6g, magnesium sulfate 1.3g, water 1100g, Semen Maydis powder 23g, maltose 13g, egg white 4g, pupa slurry 5g, green vegetables juice 1.2g;
Preparation method is:
After being mixed in proportion, each raw material of group component prepares Cordyceps militaris kind substratum, Cordyceps militaris kind substratum packs in shaking flask, every bottled enter liquid measure account for a bottle cubic capacity 40-45%, sealing sterilizing, after cooling, under aseptic condition, access Cordyceps militaris (L.) Link. slant strains, the artificial intermittently shake-flask culture 5-10 days that first leaves standstill 24-36 hour again at 22～25 DEG C, more than 3 hours observe cotton-shaped mycelia natural packing body and account for whole liquid volume time over half wait leaving standstill, obtain Cordyceps militaris bacterial classification and can be directly used in cordyceps militaris plantation through rejecting the sample of disqualified upon inspection.
More than show and described ultimate principle of the present invention, principal character and advantage of the present invention.The technician of the industry should understand; the present invention is not restricted to the described embodiments; that in above-described embodiment and specification sheets, describes just illustrates principle of the present invention; without departing from the spirit and scope of the present invention; the present invention also has various changes and modifications, and these changes and improvements all fall in the claimed scope of the invention.The claimed scope of the present invention is defined by appending claims and equivalent thereof.
Claims (3)
1. a Cordyceps militaris (L.) Link. shaking flask spawn culture composition, is characterized in that being made up of the material of following former weight part: 100～150 parts of mealy potatos, 15～20 parts of glucose, 3～5 parts of peptones, 1.5～1.8 parts of potassium primary phosphates, 1.2～1.5 parts, magnesium sulfate, 1000～1200 parts, water, 15～30 parts of Semen Maydis powder, 10～15 parts of maltose, 2～5 parts, egg white, 3～8 parts, pupa slurry, 0.5～1.5 part, green vegetables juice;
Preparation method is:
After being mixed in proportion, each raw material of group component prepares Cordyceps militaris kind substratum, Cordyceps militaris kind substratum packs in shaking flask, every bottled enter liquid measure account for a bottle cubic capacity 40-45%, sealing sterilizing, after cooling, under aseptic condition, access Cordyceps militaris (L.) Link. slant strains, the artificial intermittently shake-flask culture 5-10 days that first leaves standstill 24-36 hour again at 22～25 DEG C, more than 3 hours observe cotton-shaped mycelia natural packing body and account for whole liquid volume time over half wait leaving standstill, obtain Cordyceps militaris bacterial classification and can be directly used in cordyceps militaris plantation through rejecting the sample of disqualified upon inspection.
2. Cordyceps militaris (L.) Link. shaking flask spawn culture composition according to claim 1, is characterized in that: the feed composition also comprising in composition is expanded bean powder.
3. Cordyceps militaris (L.) Link. shaking flask spawn culture composition according to claim 2, it is characterized in that, the preparation method of described expanded bean powder is: first soybean is soaked in water 2～5 hours, in the time that appearring in soybean epidermis, fold pulls draining out, after draining, grind to form bean powder, make bean powder surface air-dry, and then bean powder is added and in extruser, carries out puffing.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201410186454.2A CN104004660A (en) | 2014-05-05 | 2014-05-05 | Shake-flask spawn culture composition of cordyceps militaris |
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| CN201410186454.2A CN104004660A (en) | 2014-05-05 | 2014-05-05 | Shake-flask spawn culture composition of cordyceps militaris |
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| CN201410186454.2A Pending CN104004660A (en) | 2014-05-05 | 2014-05-05 | Shake-flask spawn culture composition of cordyceps militaris |
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102491810A (en) * | 2011-11-17 | 2012-06-13 | 哈尔滨工业大学 | Cordyceps militaris medium |
| CN102612985A (en) * | 2011-08-29 | 2012-08-01 | 何寒 | Production technology for cordyceps militaris mycelium |
-
2014
- 2014-05-05 CN CN201410186454.2A patent/CN104004660A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102612985A (en) * | 2011-08-29 | 2012-08-01 | 何寒 | Production technology for cordyceps militaris mycelium |
| CN102491810A (en) * | 2011-11-17 | 2012-06-13 | 哈尔滨工业大学 | Cordyceps militaris medium |
Non-Patent Citations (1)
| Title |
|---|
| 河北省农业广播电视学校 组编: "《种植实用新技术问答》", 31 March 2011, 中国农业大学出版社 * |
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