CN104004644B - A kind of waste water microbial cultivation device and application - Google Patents
A kind of waste water microbial cultivation device and application Download PDFInfo
- Publication number
- CN104004644B CN104004644B CN201410215412.7A CN201410215412A CN104004644B CN 104004644 B CN104004644 B CN 104004644B CN 201410215412 A CN201410215412 A CN 201410215412A CN 104004644 B CN104004644 B CN 104004644B
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- China
- Prior art keywords
- reactor
- aeration
- waste water
- top cover
- microorganism
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/08—Flask, bottle or test tube
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/38—Caps; Covers; Plugs; Pouring means
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/06—Nozzles; Sprayers; Spargers; Diffusers
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
Abstract
The present invention provides a kind of waste water microbial cultivation device, it is made up of reactor, top cover, steel pole, two outlet on top and bottom mouth, aeration tube, pipeline, aeration head, production well, temperature regulating device, reactor is round shape, the steel pole of several extension filler it is provided with under inside top cover, top cover has several production wells, two outlet on top and bottom mouth is arranged on reactor side, and aeration tube 6 is arranged on reactor bottom, and aeration tube is by the extremely outside aeration pump of switch connecting tube. Apparatus of the present invention are used for the cultivation of pilot scale microorganism and the cultivation of biochemistry pool microorganism. The microorganism utilizing apparatus of the present invention to be tamed in laboratory is added to biochemistry pool again after reactor spreads cultivation, and bacterial classification viability strengthens, especially for high-salt wastewater. Apparatus of the present invention are reasonable in design, easy and simple to handle, practical.
Description
Technical field
The invention belongs to biological technical field, it relates to a kind of waste water microbial cultivation device, is applicable to the mass propgation of environmental microorganism, especially the enlarged culturing of brine waste microorganism.
Background technology
The biochemical treatment of waste water is the most important part in wastewater treatment, and wherein required in biochemical treatment microorganism culturing is particularly important. Generally speaking, the microorganism culturing in wastewater biochemical process becomes in the vexed exposure of biochemistry pool after mostly being and adding nutritive substance. But in the process in the face of special water, time especially in the process of saline sewage, it is necessary to cultivate special salt-durable microbe. Cultivated by multistage biomagnification, until adding biochemical treatment in biochemistry pool.
Summary of the invention
It is an object of the invention to provide a kind of waste water microbial cultivation device, by reactor, top cover, steel pole, upper water-out mouth, lower outlet, aeration tube, pipeline, aeration head, production well, temperature regulating device, switch is formed, reactor is round shape, top cover is arranged at top, in top cover, face is provided with the steel pole that 1-10 root hangs filler, hang down on every root steel pole several filler, top cover has several production wells, reactor side arranges upper water-out mouth and lower outlet, register is embedded in reactor tube walls (or hanging inside), reactor bottom arranges aeration tube, aeration tube is provided with aeration head, aeration tube is by the extremely outside aeration pump of switch connecting tube.
Described reactor material selects the one in glass, plastics, 92 bronze medals, titanium alloy or 316L stainless steel.
Another object of the present invention provides the application of described device in waste water microorganism culturing, especially the application in the enlarged culturing of brine waste microorganism.
The present invention is directed to the design of the microorganism enlarged culturing of waste water and a kind of waste water microbial cultivation device device is provided, for the cultivation of pilot scale microorganism and the cultivation of biochemistry pool microorganism, the mass propgation being applicable to environmental microorganism, the especially enlarged culturing of brine waste microorganism.The cultivation of current waste water microorganism mostly is vexed exposure after directly adding nutritive substance toward biochemistry pool, and most incubation time is long, and poor for high salt wastewater treatment efficiency. Other some wastewater biological reactor complicated operations, practical application little, less in the cultivation of high-salt wastewater microorganism. Utilizing apparatus of the present invention, the microorganism that can be tamed in laboratory is added to biochemistry pool again after reactor spreads cultivation, and bacterial classification viability strengthens, especially for high-salt wastewater. Apparatus of the present invention are reasonable in design, easy and simple to handle, practical.
Accompanying drawing explanation
Fig. 1 is present configuration schematic diagram.
Fig. 2 is aeration tube structural representation.
Embodiment
The present invention is further described in conjunction with the accompanying drawings and embodiments.
Embodiment 1
See Fig. 1, Fig. 2, the present invention provides a kind of waste water microbial cultivation device, by reactor 1, top cover 2, steel pole 3, upper water-out mouth 4, lower outlet 5, aeration tube 6, pipeline 7, aeration head 8, production well 9, temperature regulating device 10, switch 11 is formed, reactor 1 is round shape, top cover 2 is arranged at top, 1-10 root is had to hang the steel pole 3 of filler under inside top cover 2, hang down on every root steel pole 3 several filler, top cover 2 upper edge place has several production wells 9, reactor 1 side arranges upper water-out mouth 4 and lower outlet 5, register 10 is embedded on reactor 1 tube wall (or hanging inside), aeration tube 6 is set bottom reactor 1, aeration tube 6 is provided with aeration head 8, aeration tube 6 passes through switch 11 connecting tube 7 to outside aeration pump.
Described reactor 1 material selects the one in glass, plastics, 92 bronze medals, titanium alloy or 316L stainless steel.
Embodiment 2
See Fig. 1, Fig. 2, during use, first filler is suspended on steel pole 3, again nutrient solution is joined in reactor 1, the temperature needed for microorganism culturing is regulated by register 10, start outside aeration pump, air enters aeration in waste water reactor 1 by pipeline 7, nutrient solution is stirred, the seed liquor of inoculation is poured in reactor 1, cover the top cover 2 having filled filler, dissolved oxygen amount and temperature is constantly measured by register 10 during reaction, pH, the parameters such as od, to guarantee microorganism growth, after microorganism culturing is complete, close outside aeration pump, pick out microorganism from upper water-out mouth 4 or lower outlet 5 to spread cultivation liquid, cultivate for biochemical treatment and use.
Waste water with the addition of some nutritive substance after for spreading cultivation.
Embodiment 3
For preserved szechuan pickle waste water biochemical treatment cultivate time, preserved szechuan pickle waste water nutrient solution is joined in 500L reactor 1, it is adjusted to 28 DEG C by register 10, start aeration pump, aeration rate is 200L/min, and air enters reactor 1 aeration by pipeline 7, and nutrient solution is stirred, the seed liquor of inoculation is poured in reactor 1, the top cover 2 having filled filler is built. Treat that pH reaches more than 7, OD600 and reaches about 1.0, COD measured value when 200mg/L, close outside aeration pump, pick out microorganism from upper water-out mouth 4 and spread cultivation liquid, cultivate for biochemical treatment and use. The impurity such as bottom mud are discharged by lower outlet 5. Spread cultivation liquid according to the addition of 1%, can be used for the reacting weight of biochemical reaction tank 50t (see table 1).
。
Embodiment 4
For fine chemistry industry waste water biochemical treatment cultivate time, waste water nutrient solution is joined in 500L reactor 1, it is adjusted to 37 DEG C by register 10, start outside aeration pump, aeration rate is 260L/min, and air enters reactor 1 aeration by pipeline 7, and nutrient solution is stirred, the seed liquor of inoculation is poured in reactor 1, the top cover 2 having filled filler is built.Treat that OD600 reaches about 1.0, COD measured value when 500mg/L, close outside aeration pump, pick out microorganism from upper water-out mouth 4 and spread cultivation liquid, cultivate for biochemical treatment and use. The impurity such as bottom mud are discharged by lower outlet 5. The liquid that spreads cultivation can be used for the reacting weight (table 2) of biochemical reaction tank 50t.
。
Claims (3)
1. a waste water microbial cultivation device, it is characterized in that, by reactor (1), top cover (2), steel pole (3), upper water-out mouth (4), lower outlet (5), aeration tube (6), pipeline (7), aeration head (8), production well (9), register (10), switch (11) is formed, reactor (1) is round shape, top cover (2) is arranged at top, 1-10 root is had to hang the steel pole (3) of filler under top cover (2) the inside, hang down on every root steel pole (3) several filler, top cover (2) edge has several production wells (9), reactor (1) side arranges upper water-out mouth (4) and lower outlet (5), register (10) is embedded on reactor (1) tube wall or hangs inside, reactor (1) bottom arranges aeration tube (6), aeration tube (6) is provided with aeration head (8), aeration tube (6) is by the extremely outside aeration pump of switch (11) connecting tube (7), register (10) is selected to hang in reactor (1) the inside, and described waste water is brine waste.
2. a kind of waste water microbial cultivation device according to claim 1, it is characterised in that, reactor (1) material selects the one in glass, plastics, 92 bronze medals, titanium alloy or 316L stainless steel.
3. the application of a kind of waste water microbial cultivation device according to claim 1 in waste water microorganism culturing, it is characterised in that, the application in the enlarged culturing of brine waste microorganism.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410215412.7A CN104004644B (en) | 2014-05-21 | 2014-05-21 | A kind of waste water microbial cultivation device and application |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410215412.7A CN104004644B (en) | 2014-05-21 | 2014-05-21 | A kind of waste water microbial cultivation device and application |
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| Publication Number | Publication Date |
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| CN104004644A CN104004644A (en) | 2014-08-27 |
| CN104004644B true CN104004644B (en) | 2016-06-15 |
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| CN201410215412.7A Active CN104004644B (en) | 2014-05-21 | 2014-05-21 | A kind of waste water microbial cultivation device and application |
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Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104724834B (en) * | 2015-03-18 | 2017-01-04 | 中国科学院南京土壤研究所 | Periphyton processing system is utilized to remove method and the equipment thereof of copper in the sewage of source, face |
| CN106190825A (en) * | 2016-08-27 | 2016-12-07 | 浙江竟成环保科技有限公司 | Bacterial strain culture device for sewage disposal |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN201321405Y (en) * | 2008-11-10 | 2009-10-07 | 石河子爱克科技有限公司 | Microbial activating tank |
| KR20120100104A (en) * | 2011-03-03 | 2012-09-12 | 정만영 | Cultivator for effective micro-organisms |
| CN203112574U (en) * | 2012-11-29 | 2013-08-07 | 北京工业大学 | Culture device capable of fast enrichment of nitrite oxidizing bacteria (NOB) |
| CN103663696A (en) * | 2013-12-13 | 2014-03-26 | 重庆大学 | Membrane bio-reactor (MBR) used for treating preserved szechuan pickle wastewater and reinforcing biological phosphorous and nitrogen removal |
| CN203923164U (en) * | 2014-05-21 | 2014-11-05 | 浙江大学舟山海洋研究中心 | A kind of waste water microbial cultivation device |
-
2014
- 2014-05-21 CN CN201410215412.7A patent/CN104004644B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN201321405Y (en) * | 2008-11-10 | 2009-10-07 | 石河子爱克科技有限公司 | Microbial activating tank |
| KR20120100104A (en) * | 2011-03-03 | 2012-09-12 | 정만영 | Cultivator for effective micro-organisms |
| CN203112574U (en) * | 2012-11-29 | 2013-08-07 | 北京工业大学 | Culture device capable of fast enrichment of nitrite oxidizing bacteria (NOB) |
| CN103663696A (en) * | 2013-12-13 | 2014-03-26 | 重庆大学 | Membrane bio-reactor (MBR) used for treating preserved szechuan pickle wastewater and reinforcing biological phosphorous and nitrogen removal |
| CN203923164U (en) * | 2014-05-21 | 2014-11-05 | 浙江大学舟山海洋研究中心 | A kind of waste water microbial cultivation device |
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| Publication number | Publication date |
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| CN104004644A (en) | 2014-08-27 |
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Effective date of registration: 20221013 Address after: Room 602, No. 11, Baichuan Road, Lincheng Street, Zhoushan Marine Science City, Dinghai District, Zhoushan City, Zhejiang Province, 316000 Patentee after: Zhejiang Free Trade Zone Huke Biotechnology Co.,Ltd. Address before: 316021 floor 8, block A, Development Building, 171 Thousand Island Road, new town, Zhoushan, Zhejiang. Patentee before: OCEAN RESEARCH CENTER OF ZHOUSHAN, ZHEJIANG University |