CN104004644A - Wastewater microbial culture device and application thereof - Google Patents
Wastewater microbial culture device and application thereof Download PDFInfo
- Publication number
- CN104004644A CN104004644A CN201410215412.7A CN201410215412A CN104004644A CN 104004644 A CN104004644 A CN 104004644A CN 201410215412 A CN201410215412 A CN 201410215412A CN 104004644 A CN104004644 A CN 104004644A
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- reactor
- aeration
- waste water
- top cover
- microorganism
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/08—Flask, bottle or test tube
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/38—Caps; Covers; Plugs; Pouring means
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/06—Nozzles; Sprayers; Spargers; Diffusers
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
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- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Biomedical Technology (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Clinical Laboratory Science (AREA)
- Physics & Mathematics (AREA)
- Thermal Sciences (AREA)
- Analytical Chemistry (AREA)
- Biological Treatment Of Waste Water (AREA)
Abstract
The invention provides a wastewater microbial culture device consisting of a reactor, a head cover, steel poles, upper and lower water outlets, an aeration pipe, a pipeline, an aerator, air outlet holes and a temperature adjustment device, wherein the reactor is cylindrical; a plurality of steel poles used for hanging a filler are arranged at the bottom in the head cover; a plurality of air outlet holes are formed in the head cover; the upper and lower water outlets are formed in the side surface of the reactor; the aeration pipe is arranged at the bottom of the reactor, and is used for connecting the pipeline to an external aeration pump by virtue of a switch. The device disclosed by the invention is used for culture of pilot test microorganisms and biochemical pool microorganisms. By adopting the device, microorganisms acclimated in laboratories can be subjected to propagation by virtue of the reactor, and then can be put into a biochemical pool, so that the strain survivability can be enhanced by especially aiming at high-salt wastewater. The device disclosed by the invention is reasonable in design, simple and convenient in operation and strong in practicability.
Description
Technical field
The invention belongs to biological technical field, relate to a kind of waste water microbial cultivation device, be applicable to a large amount of cultivations of environmental microorganism, especially the enlarged culturing of brine waste microorganism.
Background technology
The biochemical treatment of waste water is the most important part in wastewater treatment, and wherein in biochemical treatment, required microorganism culturing is particularly important.Generally speaking, the microorganism culturing in wastewater biochemical processing forms vexed the exposing to the sun of biochemistry pool after mostly being and adding nutritive substance.Yet in the time of in the processing of processing, especially saline sewage in the face of special water, need to cultivate special salt-durable microbe.By multistage biomagnification, cultivate, until add biochemistry pool mesophytization to process.
Summary of the invention
The object of this invention is to provide a kind of waste water microbial cultivation device, by reactor, top cover, steel pole, upper water-out mouth, lower outlet, aeration tube, pipeline, aeration head, production well, temperature regulating device, switch forms, reactor is round shape, top cover is arranged at top, top cover inner face is provided with 1-10 root and hangs the steel pole that filler is used, several fillers hang down on every steel pole, on top cover, have several production wells, reactor side arranges upper water-out mouth and lower outlet, register is embedded in (or hanging over the inside) in reactor tube walls, reactor bottom arranges aeration tube, aeration tube is provided with aeration head, aeration tube passes through switch connecting tube to outside aeration pump.
Described reactor material is selected a kind of in glass, plastics, 92 bronze medals, titanium alloy or 316L stainless steel.
Another object of the present invention provides the application of described device in waste water microorganism culturing, the especially application in the enlarged culturing of brine waste microorganism.
The microorganism enlarged culturing design that the present invention is directed to waste water provides a kind of waste water microbial cultivation device device, for the cultivation of pilot scale microorganism and the cultivation of biochemistry pool microorganism, be applicable to a large amount of cultivations of environmental microorganism, especially the enlarged culturing of brine waste microorganism.The at present cultivation of waste water microorganism mostly is directly toward vexed exposing to the sun after biochemistry pool interpolation nutritive substance, and most incubation times are long, and poor for high salt wastewater treatment efficiency.Other some wastewater biological reactor complicated operations, practical application seldom, in the cultivation of high-salt wastewater microorganism still less.Utilize apparatus of the present invention, the good microorganism of Laboratory Acclimation can be added to biochemistry pool after reactor spreads cultivation again, bacterial classification viability strengthens, especially for high-salt wastewater.Apparatus of the present invention are reasonable in design, easy and simple to handle, practical.
Accompanying drawing explanation
Fig. 1 is structural representation of the present invention.
Fig. 2 is aeration tube structural representation.
embodiment
The present invention is further described in conjunction with the accompanying drawings and embodiments.
embodiment 1
Referring to Fig. 1, Fig. 2, the invention provides a kind of waste water microbial cultivation device, by reactor 1, top cover 2, steel pole 3, upper water-out mouth 4, lower outlet 5, aeration tube 6, pipeline 7, aeration head 8, production well 9, temperature regulating device 10, switch 11 forms, reactor 1 is round shape, top cover 2 is arranged at top, on under top cover 2 the insides, there is 1-10 root to hang the steel pole 3 that filler is used, several fillers hang down on every steel pole 3, top cover 2 upper edge places have several production wells 9, reactor 1 side arranges upper water-out mouth 4 and lower outlet 5, register 10 is embedded in (or hanging over the inside) on reactor 1 tube wall, reactor 1 bottom arranges aeration tube 6, aeration tube 6 is provided with aeration head 8, aeration tube 6 passes through switch 11 connecting tubes 7 to outside aeration pump.
A kind of in described reactor 1 material selection glass, plastics, 92 bronze medals, titanium alloy or 316L stainless steel.
embodiment 2
Referring to Fig. 1, Fig. 2, during use, first filler is suspended on steel pole 3, nutrient solution is joined in reactor 1 again, by register 10, regulate the required temperature of microorganism culturing, start outside aeration pump, air enters the interior aeration of waste water reactor 1 by pipeline 7, nutrient solution is stirred, the seed liquor of inoculation is poured in reactor 1, cover the top cover 2 that installs filler, during reaction, by register 10, constantly measure dissolved oxygen amount and temperature, pH, the parameters such as od, to guarantee microorganism growth, after microorganism culturing is complete, close outside aeration pump, from upper water-out mouth 4 or lower outlet 5, pick out the microorganism liquid that spreads cultivation, for biochemical treatment, cultivate and use.
Waste water has added after some nutritive substance for spreading cultivation.
embodiment 3
While cultivating for the biochemical treatment of preserved szechuan pickle waste water, preserved szechuan pickle waste water nutrient solution is joined in 500L reactor 1, by register 10, be adjusted to 28 ℃, start aeration pump, aeration rate is 200L/min, and air enters reactor 1 aeration by pipeline 7, and nutrient solution is stirred, the seed liquor of inoculation is poured in reactor 1, the top cover 2 that installs filler is built.Treat that pH reaches more than 7, OD600 reaches 1.0 left and right, and COD measured value, when 200mg/L, is closed outside aeration pump, from upper water-out mouth 4, picks out the microorganism liquid that spreads cultivation, and for biochemical treatment, cultivates and uses.The impurity such as bottom mud are discharged by lower outlet 5.Spread cultivation liquid according to 1% addition, can be used for the reacting weight (in Table 1) of biochemical reaction tank 50t.
。
embodiment 4
While cultivating for the biochemical treatment of Fine Chemistry Wastewater, waste water nutrient solution is joined in 500L reactor 1, by register 10, be adjusted to 37 ℃, start outside aeration pump, aeration rate is 260L/min, and air enters reactor 1 aeration by pipeline 7, and nutrient solution is stirred, the seed liquor of inoculation is poured in reactor 1, the top cover 2 that installs filler is built.Treat that OD600 reaches 1.0 left and right, COD measured value, when 500mg/L, is closed outside aeration pump, from upper water-out mouth 4, picks out the microorganism liquid that spreads cultivation, and for biochemical treatment, cultivates and uses.The impurity such as bottom mud are discharged by lower outlet 5.The liquid that spreads cultivation can be used for the reacting weight (table 2) of biochemical reaction tank 50t.
。
Claims (5)
1. a waste water microbial cultivation device, it is characterized in that, by reactor (1), top cover (2), steel pole (3), upper water-out mouth (4), lower outlet (5), aeration tube (6), pipeline (7), aeration head (8), production well (9), temperature regulating device (10), switch (11) forms, reactor (1) round shape, top cover (2) is arranged at top, on under top cover (2) the inside, there is 1-10 root to hang the steel pole (3) that filler is used, several fillers hang down on every steel pole (3), top cover (2) edge has several production wells (9), reactor (1) side arranges upper water-out mouth (4) and lower outlet (5), register (10) is embedded in (or hanging over the inside) on reactor (1) tube wall, reactor (1) bottom arranges aeration tube (6), aeration tube (6) is provided with aeration head (8), aeration tube (6) passes through switch (11) connecting tube (7) to outside aeration pump.
2. a kind of waste water microbial cultivation device according to claim 1, is characterized in that, register (10) selects to hang over reactor (1) the inside.
3. a kind of waste water microbial cultivation device according to claim 1, is characterized in that, a kind of in reactor (1) material selection glass, plastics, 92 bronze medals, titanium alloy or 316L stainless steel.
4. the application of a kind of waste water microbial cultivation device according to claim 1 in waste water microorganism culturing.
5. application according to claim 4, is characterized in that, the application of described device in the enlarged culturing of brine waste microorganism.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410215412.7A CN104004644B (en) | 2014-05-21 | 2014-05-21 | A kind of waste water microbial cultivation device and application |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410215412.7A CN104004644B (en) | 2014-05-21 | 2014-05-21 | A kind of waste water microbial cultivation device and application |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN104004644A true CN104004644A (en) | 2014-08-27 |
| CN104004644B CN104004644B (en) | 2016-06-15 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410215412.7A Active CN104004644B (en) | 2014-05-21 | 2014-05-21 | A kind of waste water microbial cultivation device and application |
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| CN (1) | CN104004644B (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104724834A (en) * | 2015-03-18 | 2015-06-24 | 中国科学院南京土壤研究所 | Method and equipment for removing copper in non-point source sewage employing periphyton processing system |
| CN106190825A (en) * | 2016-08-27 | 2016-12-07 | 浙江竟成环保科技有限公司 | Bacterial strain culture device for sewage disposal |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN201321405Y (en) * | 2008-11-10 | 2009-10-07 | 石河子爱克科技有限公司 | Microbial activating tank |
| KR20120100104A (en) * | 2011-03-03 | 2012-09-12 | 정만영 | Cultivator for effective micro-organisms |
| CN203112574U (en) * | 2012-11-29 | 2013-08-07 | 北京工业大学 | Culture device capable of fast enrichment of nitrite oxidizing bacteria (NOB) |
| CN103663696A (en) * | 2013-12-13 | 2014-03-26 | 重庆大学 | Membrane bio-reactor (MBR) used for treating preserved szechuan pickle wastewater and reinforcing biological phosphorous and nitrogen removal |
| CN203923164U (en) * | 2014-05-21 | 2014-11-05 | 浙江大学舟山海洋研究中心 | A kind of waste water microbial cultivation device |
-
2014
- 2014-05-21 CN CN201410215412.7A patent/CN104004644B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN201321405Y (en) * | 2008-11-10 | 2009-10-07 | 石河子爱克科技有限公司 | Microbial activating tank |
| KR20120100104A (en) * | 2011-03-03 | 2012-09-12 | 정만영 | Cultivator for effective micro-organisms |
| CN203112574U (en) * | 2012-11-29 | 2013-08-07 | 北京工业大学 | Culture device capable of fast enrichment of nitrite oxidizing bacteria (NOB) |
| CN103663696A (en) * | 2013-12-13 | 2014-03-26 | 重庆大学 | Membrane bio-reactor (MBR) used for treating preserved szechuan pickle wastewater and reinforcing biological phosphorous and nitrogen removal |
| CN203923164U (en) * | 2014-05-21 | 2014-11-05 | 浙江大学舟山海洋研究中心 | A kind of waste water microbial cultivation device |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104724834A (en) * | 2015-03-18 | 2015-06-24 | 中国科学院南京土壤研究所 | Method and equipment for removing copper in non-point source sewage employing periphyton processing system |
| CN104724834B (en) * | 2015-03-18 | 2017-01-04 | 中国科学院南京土壤研究所 | Periphyton processing system is utilized to remove method and the equipment thereof of copper in the sewage of source, face |
| CN106190825A (en) * | 2016-08-27 | 2016-12-07 | 浙江竟成环保科技有限公司 | Bacterial strain culture device for sewage disposal |
Also Published As
| Publication number | Publication date |
|---|---|
| CN104004644B (en) | 2016-06-15 |
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Effective date of registration: 20221013 Address after: Room 602, No. 11, Baichuan Road, Lincheng Street, Zhoushan Marine Science City, Dinghai District, Zhoushan City, Zhejiang Province, 316000 Patentee after: Zhejiang Free Trade Zone Huke Biotechnology Co.,Ltd. Address before: 316021 floor 8, block A, Development Building, 171 Thousand Island Road, new town, Zhoushan, Zhejiang. Patentee before: OCEAN RESEARCH CENTER OF ZHOUSHAN, ZHEJIANG University |
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