CN103992358A - Extraction method for sapindus-saponin - Google Patents
Extraction method for sapindus-saponin Download PDFInfo
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- CN103992358A CN103992358A CN201410170323.5A CN201410170323A CN103992358A CN 103992358 A CN103992358 A CN 103992358A CN 201410170323 A CN201410170323 A CN 201410170323A CN 103992358 A CN103992358 A CN 103992358A
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Abstract
The invention relates to the technical field of extraction processes for sapindus-saponin, specifically to a method for extracting sapindus-saponin from pericarp, stems and leaves of Chinese soapberry, particularly from pericarp of Chinese soapberry. The method comprises the following steps: (1) material pretreatment: a step of taking out the seeds of Chinese soapberry, placing the pericarp of Chinese soapberry in an electrothermal blowing dry box for drying at a temperature of 80 to 100 DEG C and crushing the Chinese soapberry with a pulverizer into 80-mesh powder; and (2) extraction: a step of weighing 10 g of the Chinese soapberry powder, repeatedly carrying out extraction twice, combining filtrates, using the combined filtrate as a sample extract and carrying out extraction three times with ethanol as an extraction solvent. Thus, the yield of sapindus-saponin is 9.32%. The invention has the following advantages: the method is simple, product purity is high, and a basis is provided for industrial production of sapindus-saponin and development of novel drugs; extraction efficiency is high, the solvent is nontoxic and can be easily recovered and reused, and the method is applicable to industrial production of sapindus-saponin.
Description
Technical field
The present invention relates to sapindoside extraction process technology field, specifically refer to that a kind of being applicable to extract sapindoside from Seed of Chinese Soapberry pericarp, stem, leaf, root, particularly from pericarp, extract the method for sapindoside.
Background technology
Sapindoside (Soapnut Saponin) is the extract of wild deciduous tree Seed of Chinese Soapberry (Sapindus mukorossi Gareth.) pericarp, main surface active ingredient is triterpene saponin (I), sesquiterpene glucosides class (II), fatty oil and protein, it is a kind of natural nonionic surface active agent, all-natural product, without adding any artificial synthetic detergent, essence, pigment and sanitas.There is the capillary effect of very strong reduction, for skin clean (take a shower, wash one's face, wash one's hands, have one's hair wash) and clothes washing, wash clean performance good, effectively take out stains, free from extraneous odour, antibacterial beauty treatment, brightens, nti-freckle, anti-acne, prevents and treats tetter.100% degraded, gentle non-stimulated, can not produce the residue that is harmful to HUMAN HEALTH and hostile environment.Wherein triterpene saponin, sesquiterpene glucosides class, having confirmed to act on human body skin has antibacterial, sterilization, anti-inflammatory and dandruff-removing itching-relieve effect.Also contain in addition many materials useful to human body, as: reducing sugar, lipid acid, grease, oleic acid, carotenoid, Yan acid, riboflavin, protein, 10 multiple amino acids such as vitamins C, vitamin A, vitamins B and Methionin, tyrosine, phenylalanine, L-glutamic acid, glycine and Histidine.
Sapindoside is a kind of natural nonionic surface active agent, can reduce the surface tension of water, foam is abundant, feel is fine and smooth, strong detergency, has antibacterial, environmental protection, nuisanceless, foam enriches, take out stains, antibacterial beauty treatment, tender skin, brightening, dispelling spots, dispelling, anti-acne, prevent and treat the features such as tetter.
1, sapindoside has stronger soil removability, easily degraded, and without hazard residue, rinse simple and convenient, free from extraneous odour, little to skin irritation, can be used for the washings such as skin clean (take a shower, wash one's face, wash one's hands, have one's hair wash), clothing and automobile, so it is the good raw material of shampoo, makeup and various biocleaners.
2, it has the capillary effect of very strong reduction, and human body skin is had antibacterial beauty treatment, bactericidal antiphlogistic, dandruff-removing itching-relieve, speckle removing, acne removing, brightens, prevents and treats the effects such as tetter.
3, sapindoside is also good farm chemical emulgent, and cotton aphid, red spider and sweet potato Jinhua worm etc. are all had to good killing effect.
4, sapindoside has the multiple pharmacological effect such as antitumor, antiviral, antimycotic, hypotensive.
5, sapindoside can obviously reduce volume and the concentration of hydrochloric acid in gastric juice, thereby has the potentiality for the treatment of acute gastric ulcer.
6, in sapindoside, also contain many materials useful to human body in addition, as: 10 multiple amino acids such as reducing sugar, lipid acid, grease, oleic acid, carotenoid, riboflavin, protein, vitamins C, vitamin A, vitamins B and Methionin, tyrosine, phenylalanine, L-glutamic acid, glycine and Histidine.
Summary of the invention
The object of the present invention is to provide a kind of sapindoside extracting method.
The present invention is achieved through the following technical solutions, and comprises the following steps:
(1). pretreatment, take out Seed of Chinese Soapberry seed, its pericarp is placed in to electric drying oven with forced convection, in 80～100 ℃ of oven dry, with pulverizer, be ground into 80 order powder;
(2). extract, take 10 g Seed of Chinese Soapberry powder, repeatedly extract 2 times under different extraction conditions, merging filtrate, as sample extraction thing, be take ethanol as extracting solvent, and extracting temperature is 60 ℃, and solid-liquid ratio is 1: 9, and extraction time is 3 h, and extraction time is 3 times.Under this condition, the yield of sapindoside is 9. 32%.
By 50～75% aqueous ethanolic solution refluxing extraction; Extracting solution is, purifying separated with macroporous adsorptive resins after reclaiming ethanol, thin up, tune pH3～5, to remove impurity by means of precipitation, and concentrated, dry obtain pale powder be total saponin of sapindusmukerossi; Purity >=90%, yield 5% left and right;
Described extraction and purifying are used 50～75% aqueous ethanolic solution refluxing extraction at least twice exactly, united extraction liquid, ethanol is reclaimed in underpressure distillation, thin up, adjusts pH3～5 with mineral acid, divides and abandons precipitation, clear liquid is crossed macroporous adsorptive resins, successively, with deionized water, 30～95% aqueous ethanolic solution gradient elutions, collect 70～95% ethanol eluates, the precipitation that finally reduces pressure, vacuum-drying obtain canescence total saponin of sapindusmukerossi powder-product.
Present method is equally applicable to extract total saponin of sapindusmukerossi in Seed of Chinese Soapberry stem, leaf, root.
The invention has the advantages that, adopting water extraction macroporous resin adsorption separating technology from natural Seed of Chinese Soapberry pericarp, to extract separated sapindoside, to have technique simple, product purity advantages of higher, suitability for industrialized production and new drug development that this technique is sapindoside provide foundation; Extraction efficiency is high, solvent is nontoxic and the advantage such as easy recycling, is suitable for the suitability for industrialized production of sapindoside.
Product main application: 1, for the preparation of washing product such as Liquid soap, body wash, shampoo, perfumed soap, makeup; 2, as farm chemical emulgent; 3, for antitumor, antiviral, antimycotic, Altace Ramipril; 4, as natural radioactivity organic pollutants solubilizing agent.
Embodiment
Below in conjunction with specific embodiment, the preferred embodiment of the present invention is described further:
Seed of Chinese Soapberry originates in Feidong County, Anhui Province, adopts HZ-101 type macroporous resins, and all the other reagent are analytical pure.Equipment: GFN-14 type Minitype electric pulverizers; SHB-95 type water ring vacuum pump DGF30/7-1 type electric drying oven with forced convections; UV-250 ultraviolet-visible spectrophotometer.The analysis of sapindoside is measured.The 0. 4 mg/ml hederagenin methanol solutions of take are contrast, adopt high efficiency liquid phase chromatographic analysis method to measure sapindoside content.Chromatographic condition: chromatographic column: SymmetryTM C18 (3. 9 mm * 150mm); Column temperature: 40 ℃; Moving phase: CH3 CN:H2O (H2O:90%～20%, 30 min), flow velocity: 1 ml/min; Detect wavelength: 210 nm.Sapindoside yield (%)=100% * (genuine soap glycosides output/Seed of Chinese Soapberry pericarp dry weight).
Seed of Chinese Soapberry is really cleaned, dries, peels off, pulverized, by extraction with aqueous solution, water extraction liquid is standing except crossing macroporous resin column after precipitation, first with distilled water, wash away carbohydrate impurity (effluent liquid reacts without molish), with ethanol aqueous wash deresination post, collect ethanol eluate again, underpressure distillation is except after ethanol, dry to obtain light grey powder, be product.
The extraction of sapindoside.Take 10 g Seed of Chinese Soapberry powder, under different extraction conditions, repeatedly extract 2 times, merging filtrate, as sample extraction thing (except extraction time is on the impact test of saponin(e yield), extracts solvent to investigate, extracts the impact on Seed of Chinese Soapberry yield such as temperature, extraction time, extraction time, solid-liquid ratio.
The optimal extract process of sapindoside is: take ethanol as extracting solvent, extracting temperature is 60 ℃, and solid-liquid ratio is 1: 9, and extraction time is 3 h, and extraction time is 3 times.Under this condition, the yield of sapindoside is 9. 32%.Ethanol refluxing process extracts that sapindoside has that technique is simple, extraction efficiency is high, solvent is nontoxic and the advantage such as easy recycling, is suitable for the suitability for industrialized production of sapindoside.
Claims (4)
1. a sapindoside extracting method, is characterized in that comprising the following steps:
(1). pretreatment, take out Seed of Chinese Soapberry seed, its pericarp is placed in to electric drying oven with forced convection, in 80～100 ℃ of oven dry, with pulverizer, be ground into 80 order powder;
(2). extract, take 10 g Seed of Chinese Soapberry powder, repeatedly extract 2 times under different extraction conditions, merging filtrate, as sample extraction thing, be take ethanol as extracting solvent, and extracting temperature is 60 ℃, and solid-liquid ratio is 1: 9, and extraction time is 3 h, and extraction time is 3 times.Under this condition, the yield of sapindoside is 9. 32%.
2. a kind of sapindoside extracting method according to claim 1, is characterized in that by 50～75% aqueous ethanolic solution refluxing extraction; Extracting solution is, purifying separated with macroporous adsorptive resins after reclaiming ethanol, thin up, tune pH3～5, to remove impurity by means of precipitation, and concentrated, dry obtain pale powder be total saponin of sapindusmukerossi; Purity >=90%, yield 5% left and right.
3. a kind of sapindoside extracting method according to claim 1, extraction and purifying described in it is characterized in that are used 50～75% aqueous ethanolic solution refluxing extraction at least twice exactly, united extraction liquid, ethanol is reclaimed in underpressure distillation, thin up, with mineral acid, adjust pH3～5, divide and abandon precipitation, clear liquid is crossed macroporous adsorptive resins, successively with deionized water, 30～95% aqueous ethanolic solution gradient elutions, collect 70～95% ethanol eluates, the precipitation that finally reduces pressure, vacuum-drying obtain canescence total saponin of sapindusmukerossi powder-product.
4. a kind of sapindoside extracting method according to claim 1, is characterized in that present method is equally applicable to extract total saponin of sapindusmukerossi in Seed of Chinese Soapberry stem, leaf, root.
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Cited By (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104327744A (en) * | 2014-09-23 | 2015-02-04 | 北京泰克美高新技术有限公司 | Application of Chinese soapberry extract and Chinese soapberry composition in automobile washing and washing |
| CN104725436A (en) * | 2015-01-25 | 2015-06-24 | 福建师范大学泉港石化研究院 | Production method for preventing soapberry saponin from color change in extraction process |
| CN105037445A (en) * | 2015-01-25 | 2015-11-11 | 福建师范大学泉港石化研究院 | Method of preparing light-colored sapindus saponin through oxidization-reduction combination |
| CN104292294B (en) * | 2014-08-26 | 2016-06-15 | 北京林业大学 | A kind of method of purification of sapindust saponin |
| CN105816387A (en) * | 2016-05-08 | 2016-08-03 | 杭州氧泡泡生物科技有限公司 | Pure natural solution prepared from fleece flower roots, Chinese soapberry fruits and plant Chinese honeylocust fruits |
| CN106831931A (en) * | 2017-01-23 | 2017-06-13 | 厦门大学 | A kind of method that two-step method prepares sapindoside |
| CN108659248A (en) * | 2017-03-31 | 2018-10-16 | 台湾塑胶工业股份有限公司 | Water-absorbent resin and method for producing same |
| CN108774613A (en) * | 2018-04-03 | 2018-11-09 | 浙江海洋大学 | A kind of soapberry pericarp handmade soap and preparation method thereof |
| CN108794562A (en) * | 2018-08-30 | 2018-11-13 | 佛山市欧若拉生物科技有限公司 | A kind of processing technology of sapindoside extract |
| CN109265490A (en) * | 2018-09-13 | 2019-01-25 | 湖北科技学院 | A method of improving the total saponin content of soapberry |
| CN110627843A (en) * | 2019-10-22 | 2019-12-31 | 赣州华汉生物科技有限公司 | Method for extracting saponin from soapberry pericarp |
| CN113519562A (en) * | 2021-07-28 | 2021-10-22 | 安顺学院 | Method for preparing botanical insecticide by taking rabdosia tomentosa as raw material |
| CN113813213A (en) * | 2021-11-08 | 2021-12-21 | 青岛科技大学 | Antibacterial and body taste-removing radix curcumae and soapberry composition and preparation method thereof |
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2014
- 2014-04-25 CN CN201410170323.5A patent/CN103992358A/en active Pending
Cited By (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104292294B (en) * | 2014-08-26 | 2016-06-15 | 北京林业大学 | A kind of method of purification of sapindust saponin |
| CN104327744A (en) * | 2014-09-23 | 2015-02-04 | 北京泰克美高新技术有限公司 | Application of Chinese soapberry extract and Chinese soapberry composition in automobile washing and washing |
| CN104725436A (en) * | 2015-01-25 | 2015-06-24 | 福建师范大学泉港石化研究院 | Production method for preventing soapberry saponin from color change in extraction process |
| CN105037445A (en) * | 2015-01-25 | 2015-11-11 | 福建师范大学泉港石化研究院 | Method of preparing light-colored sapindus saponin through oxidization-reduction combination |
| CN105816387B (en) * | 2016-05-08 | 2018-08-07 | 浙江艾卡医学科技有限公司 | A kind of pure natural tuber of multiflower knotweed soapberry gleditsia sinensis liquid |
| CN105816387A (en) * | 2016-05-08 | 2016-08-03 | 杭州氧泡泡生物科技有限公司 | Pure natural solution prepared from fleece flower roots, Chinese soapberry fruits and plant Chinese honeylocust fruits |
| CN106831931A (en) * | 2017-01-23 | 2017-06-13 | 厦门大学 | A kind of method that two-step method prepares sapindoside |
| CN108659248A (en) * | 2017-03-31 | 2018-10-16 | 台湾塑胶工业股份有限公司 | Water-absorbent resin and method for producing same |
| CN108659248B (en) * | 2017-03-31 | 2021-07-27 | 台湾塑胶工业股份有限公司 | Water-absorbent resin and method for producing same |
| CN108774613A (en) * | 2018-04-03 | 2018-11-09 | 浙江海洋大学 | A kind of soapberry pericarp handmade soap and preparation method thereof |
| CN108794562A (en) * | 2018-08-30 | 2018-11-13 | 佛山市欧若拉生物科技有限公司 | A kind of processing technology of sapindoside extract |
| CN109265490A (en) * | 2018-09-13 | 2019-01-25 | 湖北科技学院 | A method of improving the total saponin content of soapberry |
| CN110627843A (en) * | 2019-10-22 | 2019-12-31 | 赣州华汉生物科技有限公司 | Method for extracting saponin from soapberry pericarp |
| CN113519562A (en) * | 2021-07-28 | 2021-10-22 | 安顺学院 | Method for preparing botanical insecticide by taking rabdosia tomentosa as raw material |
| CN113813213A (en) * | 2021-11-08 | 2021-12-21 | 青岛科技大学 | Antibacterial and body taste-removing radix curcumae and soapberry composition and preparation method thereof |
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