CN109265490A - A method of improving the total saponin content of soapberry - Google Patents
A method of improving the total saponin content of soapberry Download PDFInfo
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- CN109265490A CN109265490A CN201811065426.XA CN201811065426A CN109265490A CN 109265490 A CN109265490 A CN 109265490A CN 201811065426 A CN201811065426 A CN 201811065426A CN 109265490 A CN109265490 A CN 109265490A
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- irradiation
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- C07—ORGANIC CHEMISTRY
- C07G—COMPOUNDS OF UNKNOWN CONSTITUTION
- C07G3/00—Glycosides
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Abstract
The present invention provides a kind of methods of total saponin content for improving soapberry, including by soapberry powder through electron accelerator irradiation processing step；The irradiation value of electron accelerator irradiation is ≯ 0-12kGy.The beneficial effects of the present invention are: soapberry powder, after electron accelerator irradiation is handled, radiation treatment group is significantly increased with control group total saponin content.Simultaneously within the scope of 0-6KGy, as irradiation dose increases, the bacteriostasis rate of the crude extract of soapberry powder is consequently increased.
Description
Technical field
The present invention relates to a kind of methods for improving total saponin of sapindusmukerossi with soapberry.
Background technique
Soapberry (Sapindus mukorossi Gaertn.), alias wood suffer from son, and oil suffers from son, soap tree etc..Soapberry
Contain a large amount of saponins compound in pericarp, be natural nonionic surfactant, extracting solution has very strong table
Face is active and antibacterial, anti-oxidant, removes the bioactivity such as free radical.Soapberry extract is a kind of environmentally protective washing production
Product can not have any pollution to environment by the microorganism fast decoupled in nature.
Soapberry extraction process is gradually optimized in recent years, but extract product purity it is relatively low, and because its nutrition at
Divide rich content, easily growth bacterium.Irradiation technique is a kind of emerging product processing technique.It utilizes ionising radiation (such as Co60
Gamma-rays, electron accelerator) with the interaction of substance, change the physicochemical properties of substance, and have extremely significant
Sterilization effect.
Irradiation technique be mostly used process food, the easily putrid and deteriorated product such as cosmetics, harmful substance in degradation products,
The microorganism in product is killed, to improve shelf-life and the safety of product.Studies have shown that in product after radiation treatment simultaneously
"dead" residual only changes the structure of molecule, harmless, non-toxic.The main purpose of irradiation is exactly to control micro- life
Object pollution, it can by reduce putrefactive microorganisms quantity achieve the purpose that postpone it is putrid and deteriorated, and can specific killing it
And improve the hygienic quality of food.Research shows that irradiation can make the reduction of plant cell Protoplasm viscosity, permeability increases for part,
Be conducive to substance extraction.Irradiation can also cause macromolecular substances to be degraded, at the same cause the crosslinking of part of compounds with polymerize.
Summary of the invention
The present invention provides a kind of methods of soapberry crude extract total saponin content for improving preparation.
Another goal of the invention of the invention is the soapberry crude extract for preparing to its antibacterial and decontamination performance.
The technical scheme is that
A method of the total saponin content of soapberry is improved, including soapberry powder is handled through electron accelerator irradiation
Step.
Preferably, the irradiation value of above-mentioned electron accelerator irradiation is ≯ 0-12kGy.
Preferably, the irradiation value of above-mentioned electron accelerator irradiation is 6kGy.
The beneficial effects of the present invention are: soapberry powder is after electron accelerator irradiation is handled, radiation treatment group with compare
There were significant differences for group total saponin content.
Simultaneously within the scope of 0-6KGy, as irradiation dose increases, the bacteriostasis rate of the crude extract of soapberry powder is also therewith
Increase.
Detailed description of the invention
Fig. 1 is total saponin content figure in the electron accelerator irradiation processing last extracting solution of spaonin powder；
Fig. 2 Co60- gamma-ray irradiation handles total saponin content figure in the last extracting solution of spaonin powder；
Fig. 3 various dose size electron accelerator irradiation treated soapberry crude extract washs effect to the spot on cotton
Fruit average score result figure；
Fig. 4 various dose size Co60- gamma-ray irradiation treated soapberry crude extract washs the spot on cotton
Effect average score result figure；
Wherein the order illustrated in Fig. 3 and Fig. 4 is: Tomato juice, lemon juice, soy sauce, snow chrysanthemum leaching liquor, carbon black ink,
Total detergency scoring.
Specific embodiment
Specific embodiment is further described below the present invention.
Embodiment 1
1.1 material soapberry powder of materials and methods: the baking of the soapberry pericarp in campus is picked up from self-control in November, 2017
It is dry, it crushes, seals spare；Escherichia coli (Escherichia coli), staphylococcus aureus
(Staphylococcus aureus), bacillus subtilis (Bacillus subtilis), Candida albicans (Candida
Albicans) it is purchased from Shanghai Lu Wei Science and Technology Ltd.；Commercially available bleached cotton cloth, tomato, soy sauce, lemon juice, carbon black ink, snow
Chrysanthemum；Culture dish, glass apply stick etc..
1.2 radiation treatment irradiation bombs: 1, Co60- gamma-ray irradiation source 2, electron accelerator irradiation source irradiation dose: (1) electric
Sub- accelerator: 0kGy, 4kGy, 6kGy, 8kGy, 10kGy, 12kGy.(2) Co60- gamma-rays: 0Gy, 50Gy, 100Gy, 150Gy,
200Gy.It is A liquid that the soapberry powder handled through electron accelerator irradiation, which prepares crude extract, is handled through Co60- gamma-ray irradiation
It is B liquid that soapberry powder, which prepares crude extract,.
1.3 test method
1.3.1 the preparation of soapberry crude extract takes soapberry sample 10g, adds distilled water 90ml, the high fire heating 75s of microwave,
Strainer filtering, filter residue repeat to extract twice.Merging filtrate is settled to 270ml.It is filtered under diminished pressure, obtains supernatant liquid, 4 DEG C close
Envelope saves.Every portion sample handling characteristics are same as above.
1.3.2 the measurement of saponin content
(1) assay for measuring maximum absorption wavelength total saponin of sapindusmukerossi uses the method vanillic aldehyde-of the refined foundation of Rites of the Zhou Dynasty
Glacial acetic acid development process) it carries out.Instrument: Shanghai member analyses ultraviolet-uisible spectrophotometer UV-9000S, with oleanolic acid standard
Product (purity >=98%) determine measurement wavelength of the 550nm as saponins compound in sample solution as control.
(2) using absorbance A as ordinate, concentration C/(μ g/ml) is that abscissa draws standard curve for the drafting of standard curve.
It is repeated 3 times.Calculating regression equation is y=0.0336x-0.0048, R2=0.9991.Linear relationship is good.
(3) sample measures: sapindoside crude extract being diluted, takes 100.0 μ L dilutions in test tube, remaining operation stream
Journey is identical as standard curve.And calculate total saponin content in soapberry crude extract.
1.3.3 the measurement of sapindoside crude extract detergency
(1) preparation of spot liquid: respectively by fresh tomato and lemon, shredding, grind, and filtering can be obtained western red
Persimmon juice and lemon juice；Snow chrysanthemum is taken, boiling water is added to extract, filters, obtains snow chrysanthemum leaching liquor
(2) soiled cotton makes: taking the cloth of suitable size, is cut into the rectangle that size is 5cm ╳ 5cm.Respectively in these sanctions
It is separated by dropwise addition Tomato juice at 2cm on the rectangle calico sheared, lemon juice, snow chrysanthemum leaching liquor, carbon black ink, (drop is in just for soy sauce
Centre, other in four spot drop in four vertex).The cloth of spot will be added dropwise, has been put into after being dried in drying box and takes out.
(3) soiled cotton washs: preparing the soapberry dilution that 100ml volume fraction is 3%.It is diluted to each equipped with soapberry
3 blocks of soiled cottons made are respectively put into the vial of liquid.12h is impregnated, is stirred once every 1h glass bar.It is taken out after 12h
Soiled cotton drains the water, and tiling is dried in drying box.Washing effect is observed after drying.And it scores.
(4) detergency evaluation: detergency effect is set as 5 grades, respectively A (no obvious spot residual, 80-100
Point), B (has a little spot to remain, but unobvious, 60-80 points), and C (most of spot residual, 40-60 points), D (take off few by spot
Perhaps or spot desalination, 20-40 point), E (spot is unchanged, 0-20 points) dirt essentially unchangedization afterwards).After 3 people scoring, it is averaged
Value.
1.3.4 the measurement of the measurement inhibition zone of inhibition zone is carried out referring to the method for Zhu Chaoyang [3].
Strain used: Escherichia coli, staphylococcus aureus, hay bacillus, Candida albicans, totally 4 kinds of bacterium.Bacteriostasis rate=
(test antibacterial circle diameter-control antibacterial circle diameter)/test antibacterial circle diameter × 100%.
The analysis of 1.4 data is parallel with experimental data setting 3 herein is counted, and data are united by Excel2016 software
Meter processing.
2 results and analysis
Influence of the 2.1 different irradiation to soapberry powder crude extract saponin content is to soapberry powder (valve bag packing) point
Not carry out electron accelerator irradiation, Co60- gamma-ray irradiation.Electron accelerator irradiation dosage is 0,4,6,8,10,12kGy；
Co60- gamma-ray irradiation dosage size is 0,50,100,150,200Gy.Soapberry crude extract is made after irradiation, detects total saposins
Content.As a result as shown in Fig.1 and Fig.2.As shown in Figure 1, soapberry powder is after electron accelerator irradiation is handled, radiation treatment group
There were significant differences with control group total saponin content.In the irradiation dose 0-6kGy stage, total saponin content with irradiation dose increasing
Add and increases.When irradiation dose is 6KGy, total saponin content is up to 0.67mg/ml, when irradiation dose is greater than 6kGy, total soap
Glycosides content is then reduced with the increase of irradiation dose.As irradiation dose≤6KGy, soapberry crude extract total saponin content increases
Add, is the Protoplasm viscosity for reducing soapberry powder due to ionising radiation, increases recovery rate.It increases irradiation dose and makes original
Raw matter viscosity increases.
And through Co60- gamma-ray irradiation treated soapberry powder, crude extract total saponin content increases with irradiation dose
Add no significant change, this is because a period of time that low dose irradiation causes Protoplasm viscosity reduces.Due to storage or external condition
It influences, Protoplasm viscosity is restored to original position.
Influence of the 2.2 different irradiation to soapberry powder crude extract detergency
Through electron accelerator, crude extract made from Co60- gamma-ray irradiation treated soapberry powder, through being diluted to body
After fraction 3%.For washing self-control soiled cotton.It is scored, statistics.Drafting obtains Fig. 3, Fig. 4.
The soapberry crude extract obtained after two kinds of different radiation treatments all has preferable washing to the spot on cotton
Effect.
As shown in Figure 3: A liquid, with the increase of irradiation dose, total detergency is also incremented by successively.Wherein through 6kGy irradiation at
The total decontamination force value highest of soapberry crude extract after reason, the total decontamination force value of soapberry crude extract after 10kGy radiation treatment are taken second place,
The total detergency of crude extract of soapberry powder without radiation treatment is minimum.
As shown in Figure 4: total detergency through Co60- gamma-ray irradiation treated soapberry crude extract is without significant change.
As Co60- gamma-ray irradiation dosage increases, total decontamination force value of crude extract remains constant.This is because various dose spoke
According to rear, total saponin content difference is little, therefore detergency no significant difference.
2.3 different irradiation are to the influence 2.3.1 electron accelerator irradiation of soapberry powder crude extract bacteriostasis to no trouble
The influence of sub- powder crude extract bacteriostasis
It is antibacterial by being carried out to physiological saline processing group and the soapberry powder crude extract after two kinds of different radiation treatments
Detection, the results are shown in Table 1, table 2.
As can be seen from Table 1: A liquid bacteriostasis rate dramatically increases, and the soapberry powder after the radiation treatment of 6kGy dosage is thick
Extract is maximum to the bacteriostasis rate of different strain.As irradiation dose increases, crude extract bacteriostasis rate is gradually increased, when 6kGy dosage
Up to maximum value.Analysis of variance, the bacteriostasis rate between different irradiation dose processing groups have differences, the processing of 6KGy irradiation dose
There are extremely significant differences between group and 0KGy processing group.When irradiation dose is more than 6kGy, the bacteriostasis rate of crude extract is with irradiation
Dosage increases and gradually decreases.When irradiation dose is 12kGy, crude extract bacteriostasis rate is lower than 0kGy dosage processing group.Show spoke
According to the excessively high bacteriostasis rate that may reduce soapberry powder crude extract of dosage.Therefore, when electron accelerator irradiation dosage is 6kGy,
Optimal fungistatic effect can be obtained.
Inhibiting effect of the crude extract of 1 electron accelerator irradiation of table treated soapberry powder to four kinds of bacterium
Note: small English alphabet represents 0.05 level of signifiance in table, and capitalization English letter represents 0.01 level of signifiance.
2.3.2 influence of the Co60- gamma-ray irradiation to soapberry powder crude extract bacteriostasis is as shown in Table 2, through difference
The Co60- gamma-ray irradiation of dosage size treated soapberry powder crude extract bacteriostasis rate is without significant change.
Without significant difference between Analysis of variance 50,100,150,200Gy radiation treatment group and 0Gy radiation treatment group.
Inhibiting effect of the crude extract of 2 Co60- gamma-ray irradiation of table treated soapberry powder to four kinds of bacterium
Note: small English alphabet represents 0.05 level of signifiance in table, and capitalization English letter represents 0.01 level of signifiance.
In summary, when electron accelerator irradiation dosage is 6KGy, the saponin extraction rate highest of soapberry powder, corresponding detergency and suppression
Bacterium power also reaches maximum value.As irradiation dose≤4KGy or >=8KGy, for the extraction effect of total saposins in soapberry powder
Generally.When irradiation dose is more than 10KGy, the decontamination of soapberry crude extract and the reduction of bacteriostasis are resulted even in；Low dosage
Co60- gamma-ray irradiation has no significant effect soapberry powder.As irradiation dose≤200Gy.It is total in soapberry crude extract
Saponin content, detergency, antibacterial power have no significant change.
3 conclusions
(1) low dosage Co60- gamma-ray irradiation handles soapberry powder, to the recovery rate of its total saposins without positive effect；
The processing of high dose electron accelerator irradiation can be such that total saposins recovery rate in the crude extract of spaonin powder end increases, when being lower than 6KGy,
Its total saponin content increases with the increase of irradiation dose, but its content increases decline therewith again when more than 6KGy.This experiment
In processing group, when irradiation dose is 6KGy, total saponin content highest in soapberry powder crude extract.
(2) low dosage Co60- gamma-ray irradiation has no significant effect total detergency of the crude extract of soapberry powder.Through
The total detergency of crude extract of≤200Gy dose delivery treated soapberry powder, equal no significant difference between each processing group；
The electron accelerator irradiation of high dose handles soapberry powder, and crude extract dirt-removing power obtained significantly improves.At 6KGy irradiation
The total detergency highest of reason group, when irradiation dose is more than or less than 6KGy, downward trend is presented in detergency.
(3) low dosage Co60- gamma-ray irradiation is for the antibacterial power of the crude extract of soapberry powder also without obvious effect.When
When handling soapberry powder using electron accelerator irradiation, within the scope of 0-6KGy, as irradiation dose increases, soapberry powder
The bacteriostasis rate of crude extract be consequently increased.When irradiation dose reaches 6KGy, bacteriostasis rate reaches maximum value.When irradiation dose is more than
When 6KGy, within the scope of 6-12KGy, as irradiation dose increases, the bacteriostasis rate of the crude extract of soapberry powder is decreased, when
When irradiation dose is more than 10KGy, the bacteriostasis rate of soapberry crude extract may be inhibited.
Claims (3)
1. a kind of method for the total saponin content for improving soapberry, which is characterized in that including accelerating soapberry powder through electronics
Device radiation treatment step.
2. the method as described in claim 1, which is characterized in that the irradiation value of the electron accelerator irradiation is ≯ 0-12kGy.
3. the method as described in claim 1, which is characterized in that the irradiation value of the electron accelerator irradiation is 6kGy.
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103992358A (en) * | 2014-04-25 | 2014-08-20 | 湖南春源生物科技开发集团有限公司 | Extraction method for sapindus-saponin |
| CN104773338A (en) * | 2015-04-04 | 2015-07-15 | 湖北科技学院 | Anaerobic packing low-temperature and high-dosage-rate irradiation sterilization method of gelatin |
| CN107028994A (en) * | 2016-12-16 | 2017-08-11 | 江苏苏轩堂药业有限公司 | The direct oral traditional Chinese medicine medicine materical crude slice of Radix Notoginseng powder |
-
2018
- 2018-09-13 CN CN201811065426.XA patent/CN109265490A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103992358A (en) * | 2014-04-25 | 2014-08-20 | 湖南春源生物科技开发集团有限公司 | Extraction method for sapindus-saponin |
| CN104773338A (en) * | 2015-04-04 | 2015-07-15 | 湖北科技学院 | Anaerobic packing low-temperature and high-dosage-rate irradiation sterilization method of gelatin |
| CN107028994A (en) * | 2016-12-16 | 2017-08-11 | 江苏苏轩堂药业有限公司 | The direct oral traditional Chinese medicine medicine materical crude slice of Radix Notoginseng powder |
Non-Patent Citations (1)
| Title |
|---|
| 章月红等，: "西洋参辐照效应的研究", 《核农学报》 * |
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