CN103990503B - Disposable liquid-transfering gun coating gun head and using method thereof - Google Patents
Disposable liquid-transfering gun coating gun head and using method thereof Download PDFInfo
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- CN103990503B CN103990503B CN201410207441.9A CN201410207441A CN103990503B CN 103990503 B CN103990503 B CN 103990503B CN 201410207441 A CN201410207441 A CN 201410207441A CN 103990503 B CN103990503 B CN 103990503B
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- thread pipe
- intermediate thread
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Abstract
The present invention relates to a kind of disposable liquid-transfering gun coating gun head and using method thereof, this rifle head and liquid-transfering gun with the use of, interface portion, intermediate thread pipe, rifle head body.Interface portion, intermediate thread pipe, rifle head body three part are suitable for connecting successively according to the order of interface portion, intermediate thread pipe, rifle head body, and interface portion is also applicable to be combined with the insertion section of pipettor; This interface portion is truncated cone-shaped or cylindrical, and surface is smooth surface; Its making material used of interface portion, intermediate thread pipe, rifle head body is transparent or translucent; This intermediate thread pipe by certain toughness and deformable material form, its material is consistent with interface portion and rifle head body, or be as required suitable for other material, its external diameter internal diameter is homogeneous up and down.Disposable coating gun head involved in the present invention has and moves liquid and coating two kinds of functions, and easy and simple to handle, flexible, high temperature resistant, high pressure resistant, disposable, time and labour saving, pollutes few, and consumption is large, Yong Huguang.The invention still further relates to using method and the step of disposable liquid-transfering gun coating gun head.
Description
Technical field
The invention belongs to life science/biological field, relate to the concrete technical field of microbiology, molecular biology experiment apparatus and use, particularly relate to a kind of disposable liquid-transfering gun coating gun head for experimental liquid coating and using method thereof.
Background technology
In Microbiology Experiment and molecular biology experiment, the use of spreading rod is comparatively extensive, and as common L-type spreading rod, and common rifle head is also frequent replacing, and consumption is very big.Current flat board coating be spreading rod, and spreading rod material to be generally be glass or stainless.
There is very large defect in experiment appliance so at present: the drawback of spreading rod own is a lot, must under the calcination of flame when glass spreading rod uses, and high temperature is easy to grip not, and easily smashes; Stainless spreading rod due to iron and steel specific heat too little, after heating up, heat radiation is slowly, meaningless waits for too long, and spreading rod is all coated with and once needs to burn once, uses and comparatively bothers.The present invention can overcome above shortcoming, and advantage is: 1, rifle head is after removing sealing shroud, can bend, and what its original state can possess common rifle head moves liquid function, may be used for coating after stretch bending; 2, rifle head as spreading rod, also can move liquid; 3, rifle head is plastic products, and disposable, production prices are cheap, more cost-saving than spreading rod; 4, can autoclaving; 5, in use procedure without flame calcination; 6, rifle head instead of rifle head in the past and spreading rod, fast convenient and simple for operation, time saving and energy saving; 7, there is the liquid-transfering gun function of general rifle head; 8, can reduce and drop pollutions such as spilling; 9, the every field of liquid extraction coating is applicable to.10, can support the use with standard liquid-transfering gun rifle, have a wide range of applications field.
In a word, disposable liquid-transfering gun coating gun head is easy to operation, and time saving and energy saving, Painting effect is good, and can move liquid and also can be coated with, sterile working is secure.
Summary of the invention
The present invention proposes a kind of disposable liquid-transfering gun coating gun head, with liquid-transfering gun with the use of, and use its coating process:
Coating gun header structure composition comprises: interface portion, intermediate thread pipe, rifle head body.
Its interface portion, intermediate thread pipe, rifle head body three part are suitable for connecting successively according to the order of interface portion, intermediate thread pipe, rifle head body, and interface portion is also applicable to be combined with the insertion section of pipettor; The internal diameter of interface portion and the internal diameter of general standard pipettor cooperatively interact; This interface portion is truncated cone-shaped or cylindrical, and surface is smooth surface; During for truncated cone-shaped, the oral area bore connected with pipettor is larger.
This intermediate thread pipe by certain toughness and deformable material form, its external diameter internal diameter is homogeneous up and down; Its material can be polyurethane material, as TPU etc.
This rifle head body is taper, and liquid outlet bore is comparatively thin, comparatively thick with intermediate thread pipe junction bore, smooth outer surface.
Interface portion, intermediate thread pipe, rifle head body are made by high pressure resistant material, and the making material that it uses is transparent or translucent; The material of intermediate thread pipe can be consistent with interface portion and rifle head body; Also other material can be suitable for as required; Intermediate thread pipe has the sealing shroud that can simply remove, and intermediate thread pipe is initially in the full deflated state of screw thread closely, and overcoat sealing shroud simultaneously, can stretch after removing sealing shroud, retract, and bending certain angle; Sealing shroud and intermediate thread pipe are identical material, between have many places sheets to connect, sheet junction all has the opening being easy to be separated, and is convenient under common External Force Acting, make sealing shroud be separated with intermediate thread pipe; Backbone screwed pipe in the full deflated state of screw thread closely, its structure and general rifle head do not have large difference, can bear commonly to move liquid task dispatching.
The connection of interface portion and intermediate thread pipe, and the connection of intermediate thread pipe and rifle head body, can be that adhesive type is tightly connected, also can be that screw-type is tightly connected, or the screw-type of band O-ring seal is tightly connected, here, those skilled in the art can select connected mode according to specific needs, as experiment liquid, requirement for experiment condition are not high, then can be tightly connected with adhesive type, if harsher to requirement for experiment condition, then can the screw-type of select tape O-ring seal be tightly connected; Its material for interface portion, intermediate thread pipe, rifle head body is plastics, and color is colourless or possesses certain color.
Its design parameter being applicable to the disposable liquid-transfering gun coating gun head of the rifle head of 200 μ l liquid-transfering gun specifications is: interface portion length 1-2cm, and opening portion internal diameter 5-6mm, external diameter 7-8mm, thickness 1-2mm, for inserting liquid-transfering gun; Intermediate thread pipe retractable curved 90, length 5mm-1cm, intracavity diameter 3-4mm, external diameter 5-6mm; Rifle head body: taper length 3-4cm, conical upper chamber diameter 3-4mm, external diameter 4-6mm, thickness 1-2mm.
It is applicable to the design parameter of the disposable liquid-transfering gun coating gun head of the rifle head of 1000 μ l liquid-transfering gun specifications: interface portion length 1-2cm, and opening portion internal diameter 5-6mm, external diameter 7-8mm, thickness 1-2mm, for inserting liquid-transfering gun; Intermediate thread pipe retractable curved 90, length 1-2cm, intracavity diameter 4-5mm, external diameter 6-7mm; Rifle head body: taper, length 4-6cm, conical upper chamber diameter 5-6mm, external diameter 7-8mm, thickness 1-2mm.
The material of interface portion, intermediate thread pipe, rifle head body can be PP, polyurethane material, PP respectively, and sealing shroud can be any just convenient plastic material removed of can slightly exerting oneself.
What make use of disposable liquid-transfering gun coating gun head of the present invention moves liquid using method, comprises the following steps:
1) choose the liquid-transfering gun of appropriate size, liquid-transfering gun is inserted interface portion, keep compact siro spinning technology;
2) with liquid-transfering gun pipette samples liquid to the amount needed, pipette amount of liquid and be no more than the amount of liquid that liquid-transfering gun specification can hold;
3) utilize rifle head, imbitition is dripped to plating medium surface: the amount of liquid oozed here is the amount being suitable for being coated with, specifically according to the difference of object liquid detected, the difference of experiment purpose and different;
4) rifle head body tip is withstood on plate lid inner face, allow rifle head intermediate thread bend portions, allow rifle tilt simultaneously, angled with media surface, previously, push-and-pull for several times, and then rotary coating, can complete the even spread of liquid like this;
5), after being coated with, liquid-transfering gun rifle head is discarded;
Operation in step (4) is specially: rifle head body tip is withstood on plate lid inner face, allows rifle head intermediate thread bend portions 90, allows rifle tilt simultaneously, 30-45 ° is become with media surface, push-and-pull 2-3 time previously, and then rotary coating, can complete the even spread of bacterium liquid like this; The selected liquid-transfering gun specification that can coordinate can be two kinds of specifications of 200 μ l or 1000 μ l, also can choose other common specification liquid-transfering guns.As 400,600,800ul etc.
accompanying drawing illustrates:specifically see specification digest accompanying drawing 1.The structure composition of accompanying drawing 1 disposable liquid-transfering gun coating gun head.1. interface portion 2. intermediate thread pipe 3. rifle head body.
detailed description of the invention:
Employ disposable liquid-transfering gun coating gun head, its composition comprises interface portion, intermediate thread pipe, rifle head body.
Interface portion, intermediate thread pipe, rifle head body three part are suitable for connecting successively according to the order of interface portion, intermediate thread pipe, rifle head body, and interface portion is also applicable to be combined with the insertion section of pipettor.
This interface portion is cylindrical, and surface is smooth surface; This intermediate thread pipe by certain toughness and deformable material form, its external diameter internal diameter is homogeneous up and down.
This rifle head body is taper, and liquid outlet bore is comparatively thin, comparatively thick with intermediate thread pipe junction bore, smooth outer surface.
Interface portion, intermediate thread pipe, rifle head body are made by high pressure resistant material, and the making material that it uses is transparent or translucent.The material of intermediate thread pipe can be consistent with interface portion and rifle head body; Also other material can be suitable for as required.
Intermediate thread pipe has the sealing shroud that can simply remove, and intermediate thread pipe is initially in the full deflated state of screw thread closely, and overcoat sealing shroud simultaneously, can stretch after removing sealing shroud, retract, and bending certain angle.
The connection of interface portion and intermediate thread pipe, and the connection of intermediate thread pipe and rifle head body are that adhesive type is tightly connected.
Material for interface portion, intermediate thread pipe, rifle head body is plastics, and color is milky.
This rifle head is the disposable liquid-transfering gun coating gun head of the rifle head of applicable 200 μ l liquid-transfering gun specifications, design parameter:
Interface portion length 1-2cm, opening portion internal diameter 5-6mm, external diameter 7-8mm, thickness 1-2mm, for inserting liquid-transfering gun; Intermediate thread pipe retractable curved 90, length 5mm-1cm, intracavity diameter 3-4mm, external diameter 5-6mm; Rifle head body: taper length 3-4cm, conical upper chamber diameter 3-4mm, external diameter 4-6mm, thickness 1-2mm.
The material of interface portion, intermediate thread pipe, rifle head body is PP, polyurethane material, PP respectively.
Its using method of disposable liquid-transfering gun coating gun head, comprises the following steps and 1. chooses required rifle head, liquid-transfering gun is inserted upper interface portions, keeps compact siro spinning technology; 2. by the amount that liquid-transfering gun pipette samples liquid extremely needs, pipette amount of liquid and be no more than 200 μ l; 3. imbitition is dripped to plating medium surface: 4. rifle head body tip is withstood on plate lid inner face, allow rifle head intermediate thread pipe bend 90, allow rifle tilt simultaneously, at 45 ° with media surface, push-and-pull 3 times previously, and then rotary coating, can complete the even spread of bacterium liquid like this.5., after being coated with, liquid-transfering gun rifle head is discarded.
Show by using result: disposable coating gun head involved in the present invention has and moves liquid and coating two kinds of functions, and easy and simple to handle, flexible, high temperature resistant, high pressure resistant, disposable, time and labour saving, pollutes few, and consumption is large, Yong Huguang.
The above, in order to technical scheme of the present invention to be described, is not intended to limit; Although with reference to foregoing teachings to invention has been detailed description, those of ordinary skill in the art is to be understood that: it still can be modified to aforementioned described technical scheme and related content, or carries out equivalent replacement to wherein portion of techniques feature; And these amendments or replacement, do not make the essence of appropriate technical solution depart from the spirit and scope of embodiment of the present invention technical scheme.
Claims (8)
1. a disposable liquid-transfering gun coating gun head, is characterized in that this coating gun header structure composition comprises:
Interface portion, intermediate thread pipe, rifle head body;
Interface portion, intermediate thread pipe, rifle head body three part are suitable for connecting successively according to the order of interface portion, intermediate thread pipe, rifle head body, and interface portion is also applicable to be combined with the insertion section of pipettor;
This interface portion is truncated cone-shaped or cylindrical, and surface is smooth surface;
Its making material used of interface portion, intermediate thread pipe, rifle head body is transparent or translucent;
This intermediate thread pipe by certain toughness and deformable material form, its material is consistent with interface portion and rifle head body, or be as required suitable for other material, its external diameter internal diameter is homogeneous up and down;
This rifle head body is taper, and liquid outlet bore is comparatively thin, comparatively thick with intermediate thread pipe junction bore, smooth outer surface;
There is the sealing shroud that can simply remove, intermediate thread pipe initially in the full deflated state of screw thread closely, overcoat sealing shroud simultaneously, after removing sealing shroud, intermediate thread pipe can be stretched, retract, and bending certain angle;
The connection of interface portion and intermediate thread pipe, and the connected mode of intermediate thread pipe and rifle head body, be that adhesive type is tightly connected, screw-type is tightly connected, or the screw-type of band O-ring seal is tightly connected three kinds of optional one of connected mode.
2. a kind of disposable liquid-transfering gun coating gun head as claimed in claim 1, also possesses feature:
Material for interface portion, intermediate thread pipe, rifle head body is plastics, and color is colourless or possesses certain color.
3. a kind of disposable liquid-transfering gun coating gun head as claimed in claim 2, also possesses feature:
Be applicable to the design parameter of the disposable liquid-transfering gun coating gun head of the rifle head of 200 μ l liquid-transfering gun specifications:
Interface portion length 1-2cm, opening portion internal diameter 5-6mm, external diameter 7-8mm, thickness 1-2mm, for inserting liquid-transfering gun;
Intermediate thread pipe retractable curved 90, length 5mm-1cm, intracavity diameter 3-4mm, external diameter 5-6mm;
Rifle head body: taper length 3-4cm, conical upper chamber diameter 3-4mm, external diameter 4-6mm, thickness 1-2mm.
4. a kind of disposable liquid-transfering gun coating gun head as claimed in claim 2, also possesses feature:
Be applicable to the design parameter of the disposable liquid-transfering gun coating gun head of the rifle head of 1000 μ l liquid-transfering gun specifications:
Interface portion length 1-2cm, opening portion internal diameter 5-6mm, external diameter 7-8mm, thickness 1-2mm, for inserting liquid-transfering gun;
Intermediate thread pipe retractable curved 90, length 1-2cm, intracavity diameter 4-5mm, external diameter 6-7mm;
Rifle head body: taper length 4-6cm, conical upper chamber diameter 5-6mm, external diameter 7-8mm, thickness 1-2mm.
5. the disposable liquid-transfering gun coating gun of the one as described in claim 3 or 4 head, also possesses feature:
The material of interface portion, intermediate thread pipe, rifle head body is PP, polyurethane material, PP respectively.
6. one kind make use of claim 1-5 arbitrary described disposable liquid-transfering gun coating gun head
move liquid coating process, comprise the following steps:
1) choose the liquid-transfering gun of appropriate size, liquid-transfering gun is inserted interface portion, keep compact siro spinning technology;
2) with liquid-transfering gun pipette samples liquid to the amount needed, pipette amount of liquid and be no more than the amount of liquid that liquid-transfering gun specification can hold;
3) utilize rifle head, imbitition dripped to plating medium surface:
4) rifle head body tip is withstood on plate lid inner face, allow rifle head intermediate thread bend portions, allow rifle tilt simultaneously, angled with media surface, previously, push-and-pull for several times, and then rotary coating, namely completes the even spread of liquid;
5), after being coated with, liquid-transfering gun rifle head is discarded.
7. move liquid coating process as claimed in claim 6, it is characterized in that:
The operation of abovementioned steps (4) is specially: rifle head body tip is withstood on plate lid inner face, allows rifle head intermediate thread bend portions 90, allows rifle tilt simultaneously, 30-45 ° is become with media surface, push-and-pull 2-3 time previously, and then rotary coating, namely complete the even spread of bacterium liquid.
8. as claimed in claims 6 or 7 move liquid coating process, it is characterized in that:
The liquid-transfering gun specification of selected cooperation can be two kinds of specifications of 200 μ l or 1000 μ l.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410207441.9A CN103990503B (en) | 2014-05-16 | 2014-05-16 | Disposable liquid-transfering gun coating gun head and using method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410207441.9A CN103990503B (en) | 2014-05-16 | 2014-05-16 | Disposable liquid-transfering gun coating gun head and using method thereof |
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| Publication Number | Publication Date |
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| CN103990503A CN103990503A (en) | 2014-08-20 |
| CN103990503B true CN103990503B (en) | 2015-09-09 |
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| CN201410207441.9A Expired - Fee Related CN103990503B (en) | 2014-05-16 | 2014-05-16 | Disposable liquid-transfering gun coating gun head and using method thereof |
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Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2016153743A (en) * | 2015-02-20 | 2016-08-25 | セイコーエプソン株式会社 | Container, liquid storage body, cartridge and manufacturing method of liquid storage body |
| CN107325961A (en) * | 2017-06-23 | 2017-11-07 | 湖北省农业科学院果树茶叶研究所 | Suction head type bacterium apparatus for coating |
| CN108395982B (en) * | 2018-05-30 | 2024-04-23 | 厦门市科环海洋生物科技有限公司 | Colony spraying device |
| CN109499637A (en) * | 2019-01-27 | 2019-03-22 | 刘晓 | A kind of bent angle liquid relief plastics pipette tips |
| CN109777727A (en) * | 2019-03-11 | 2019-05-21 | 江西农业大学 | A kind of portable microbial suspension coating pipette tips and its design method |
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| GB738927A (en) * | 1952-09-26 | 1955-10-19 | Andre Savary | Improvements in or relating to dropper closures |
| WO1999006149A1 (en) * | 1997-08-01 | 1999-02-11 | Aalto Scientific, Ltd. | Pipetting devices preloaded with standardized control sample materials |
| US6599755B1 (en) * | 1999-04-27 | 2003-07-29 | Basf Aktiengesellschaft | Method and device for applying small quantities of liquid |
| CN101076406A (en) * | 2004-10-16 | 2007-11-21 | 奥林巴斯生命及材料科学欧洲有限公司 | Pipetting device |
| CN102365129A (en) * | 2009-03-24 | 2012-02-29 | 恩姆菲舍尔科技公司 | Tip part pipette |
| CN202366724U (en) * | 2011-12-23 | 2012-08-08 | 重庆卓驰生物科技有限公司 | Elbow transfer pipette |
| CN102698824A (en) * | 2012-06-07 | 2012-10-03 | 林昂 | Novel liquid transfer device |
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2014
- 2014-05-16 CN CN201410207441.9A patent/CN103990503B/en not_active Expired - Fee Related
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB738927A (en) * | 1952-09-26 | 1955-10-19 | Andre Savary | Improvements in or relating to dropper closures |
| WO1999006149A1 (en) * | 1997-08-01 | 1999-02-11 | Aalto Scientific, Ltd. | Pipetting devices preloaded with standardized control sample materials |
| US6599755B1 (en) * | 1999-04-27 | 2003-07-29 | Basf Aktiengesellschaft | Method and device for applying small quantities of liquid |
| CN101076406A (en) * | 2004-10-16 | 2007-11-21 | 奥林巴斯生命及材料科学欧洲有限公司 | Pipetting device |
| CN102365129A (en) * | 2009-03-24 | 2012-02-29 | 恩姆菲舍尔科技公司 | Tip part pipette |
| CN202366724U (en) * | 2011-12-23 | 2012-08-08 | 重庆卓驰生物科技有限公司 | Elbow transfer pipette |
| CN102698824A (en) * | 2012-06-07 | 2012-10-03 | 林昂 | Novel liquid transfer device |
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| CN103990503A (en) | 2014-08-20 |
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Granted publication date: 20150909 Termination date: 20160516 |