CN103990503A - Disposable pipette coating tip and using method thereof - Google Patents
Disposable pipette coating tip and using method thereof Download PDFInfo
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- CN103990503A CN103990503A CN201410207441.9A CN201410207441A CN103990503A CN 103990503 A CN103990503 A CN 103990503A CN 201410207441 A CN201410207441 A CN 201410207441A CN 103990503 A CN103990503 A CN 103990503A
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- liquid
- rifle head
- transfering gun
- intermediate thread
- thread pipe
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Abstract
The invention relates to a disposable pipette coating tip and a using method thereof. The tip is matched with a pipette, and comprises a connector part, an intermediate threaded pipe and a tip body. The connector body, the intermediate threaded pipe and the tip body are connected in sequence; the tip body is also applicable to being combined with an insertion part of the pipette; the connector part is frustum-shaped or cylindrical, and the surface of the connector part is a smooth surface; the connector part, the intermediate threaded pipe and the tip body are made of transparent or semitransparent manufacturing materials; the intermediate threaded pipe is made of a material which has certain toughness and is deformable, is made of the same material as the connector part and the tip body, or can be applicable to other materials as required; the inside diameter and the outside diameter of the intermediate threaded pipe are uniform vertically. The disposable pipette coating tip has two functions of transferring liquid and coating, and is easy and convenient to operate, bendable, high-temperature resistant, disposable, time-saving, labor-saving, low in pollution, large in using amount and wide in users. The invention also relates to the using method and using steps of the disposable pipette coating tip.
Description
Technical field
The invention belongs to life science/biological field, relate to the concrete technical field of microbiology, molecular biology experiment apparatus and use, relate in particular to a kind of disposable liquid-transfering gun coating gun head and using method thereof for experimental liquid coating.
Background technology
In Microbiology Experiment and molecular biology experiment, the use of spreading rod is comparatively extensive, and as common L-type spreading rod, and common rifle head is also frequent replacing, and consumption is very big.At present dull and stereotyped coating use is spreading rod, and spreading rod material be generally be glass or stainless.
There is very large defect at present such experiment appliance: the drawback of spreading rod own is a lot, must be under the calcination of flame when glass spreading rod uses, and high temperature is easy to grip not, and easily smashes; Stainless spreading rod is because iron and steel specific heat is too little, after heating up heat radiation slowly, meaningless waits for too long, and spreading rod is all coated with and once needs to burn once, uses comparatively trouble.The present invention can overcome above shortcoming, and advantage is: 1, rifle head is removing after sealing shroud, can be bending, and what its original state can possess common rifle head moves liquid function, can be for coating after stretch bending; 2, rifle head can be used as spreading rod, also can move liquid; 3, rifle head is plastic products, disposable, and production prices are cheap, more cost-saving than spreading rod; 4, can autoclaving; 5, in use procedure without flame calcination; 6, a rifle head has substituted rifle head and spreading rod in the past, convenient and simple for operation quick, time saving and energy saving; 7, there is the liquid-transfering gun function of general rifle head; 8, can reduce to drop and the pollution such as spill; 9, be applicable to the every field of liquid extraction coating.10, can support the use with standard liquid-transfering gun rifle, have a wide range of applications field.
In a word, disposable liquid-transfering gun coating gun head easy operating, time saving and energy saving, Painting effect is good, can move liquid and also can be coated with, and sterile working is secure.
Summary of the invention
The present invention proposes a kind of disposable liquid-transfering gun coating gun head, is used in conjunction with, and uses its coating process with liquid-transfering gun:
Coating gun header structure composition comprises: interface portion, intermediate thread pipe, rifle head body.
Its interface portion, intermediate thread pipe, rifle head body three parts are suitable for connecting successively according to the order of interface portion, intermediate thread pipe, rifle head body, and interface portion is also applicable to be combined with the insertion section of pipettor; The internal diameter of the internal diameter of interface portion and general standard pipettor cooperatively interacts; This interface portion is truncated cone-shaped or cylindrical, and surface is smooth surface; During for truncated cone-shaped, the oral area bore joining with pipettor is larger.
This intermediate thread pipe is made up of certain toughness and deformable material, the upper and lower homogeneous of its external diameter internal diameter; Its material can be polyurethane material, as TPU etc.
This rifle head body is taper, and liquid outlet bore is thinner, thicker with intermediate thread pipe junction bore, smooth outer surface.
Interface portion, intermediate thread pipe, rifle head body are made by high pressure resistant material, and the making material that it uses is transparent or translucent; The material of intermediate thread pipe can be consistent with interface portion and rifle head body; Also can be suitable for as required other material; Intermediate thread pipe has the sealing shroud that can simply remove, and intermediate thread pipe is initially the full deflated state of screw thread closely, and overcoat sealing shroud simultaneously can stretch, retract after removal sealing shroud, and bending certain angle; Sealing shroud and intermediate thread pipe are identical material, between have many places sheets to connect, sheet junction all has and is easy to the opening that separates, is convenient to make sealing shroud to separate with intermediate thread pipe under common External Force Acting; Be the backbone screwed pipe of the full deflated state of screw thread closely, its structure does not have large difference with general rifle head, can bear the common liquid task dispatching that moves.
Interface portion is connected with intermediate thread pipe, and being connected of intermediate thread pipe and rifle head body, can be that adhesive type is tightly connected, can be also that screw-type is tightly connected, or be tightly connected with the screw-type of O-ring seal, here, those skilled in the art can select connected mode according to specific needs, as not high with liquid, requirement for experiment condition in experiment, can be tightly connected with adhesive type, if harsher to requirement for experiment condition, screw-type that can select tape O-ring seal is tightly connected; Its material for interface portion, intermediate thread pipe, rifle head body is plastics, and color is colourless or possesses certain color.
Its design parameter that is applicable to the disposable liquid-transfering gun coating gun head of the rifle head of 200 μ l liquid-transfering gun specifications is: interface portion length 1-2cm, and peristome internal diameter 5-6mm, external diameter 7-8mm, thickness 1-2mm, for inserting liquid-transfering gun; Intermediate thread pipe is retractable curved 90, length 5mm-1cm, intracavity diameter 3-4mm, external diameter 5-6mm; Rifle head body: taper length 3-4cm, conical upper chamber diameter 3-4mm, external diameter 4-6mm, thickness 1-2mm.
It is applicable to the design parameter of the disposable liquid-transfering gun coating gun head of the rifle head of 1000 μ l liquid-transfering gun specifications: interface portion length 1-2cm, and peristome internal diameter 5-6mm, external diameter 7-8mm, thickness 1-2mm, for inserting liquid-transfering gun; Intermediate thread pipe is retractable curved 90, length 1-2cm, intracavity diameter 4-5mm, external diameter 6-7mm; Rifle head body: taper, length 4-6cm, conical upper chamber diameter 5-6mm, external diameter 7-8mm, thickness 1-2mm.
The material of interface portion, intermediate thread pipe, rifle head body can be respectively PP, polyurethane material, PP, and sealing shroud can be any just convenient plastic material of removing of can slightly exerting oneself.
That has utilized disposable liquid-transfering gun coating gun head of the present invention moves liquid using method, comprises the following steps:
1) choose the liquid-transfering gun of appropriate size, liquid-transfering gun is inserted to interface portion, keep closely connecting;
2) draw sample liquids to the amount needing with liquid-transfering gun, pipette amount of liquid and be no more than the amount of liquid that liquid-transfering gun specification can be held;
3) utilize rifle head, imbitition is dripped to plate media surface: the amount of liquid oozing is here the amount that is suitable for coating, specifically according to the difference of difference, the experiment purpose of the object liquid detecting and different;
4) rifle head body tip is withstood on to plate lid inner face, allows the bending of rifle head intermediate thread part, allow rifle tilt simultaneously, angled with media surface, after push-and-pull for several times, and then rotary coating, can complete the even coating of liquid like this;
5), after being coated with, liquid-transfering gun rifle head is discarded;
Operation in step (4) is specially: rifle head body tip is withstood on to plate lid inner face, allow rifle head intermediate thread part bending 90, allow rifle tilt simultaneously, become 30-45 ° with media surface, push-and-pull 2-3 time previously, and then rotary coating, can complete the even coating of bacterium liquid like this; The selected liquid-transfering gun specification that can coordinate can be two kinds of specifications of 200 μ l or 1000 μ l, also can choose other common specification liquid-transfering guns.As 400,600,800ul etc.
brief description of the drawings:specifically see specification digest accompanying drawing 1.The structure composition of the disposable liquid-transfering gun coating gun of accompanying drawing 1 head.1. interface portion 2. intermediate thread pipe 3. rifle head bodies.
detailed description of the invention:
Used disposable liquid-transfering gun coating gun head, its composition comprises interface portion, intermediate thread pipe, rifle head body.
Interface portion, intermediate thread pipe, rifle head body three parts are suitable for connecting successively according to the order of interface portion, intermediate thread pipe, rifle head body, and interface portion is also applicable to be combined with the insertion section of pipettor.
This interface portion is cylindrical, and surface is smooth surface; This intermediate thread pipe is made up of certain toughness and deformable material, the upper and lower homogeneous of its external diameter internal diameter.
This rifle head body is taper, and liquid outlet bore is thinner, thicker with intermediate thread pipe junction bore, smooth outer surface.
Interface portion, intermediate thread pipe, rifle head body are made by high pressure resistant material, and the making material that it uses is transparent or translucent.The material of intermediate thread pipe can be consistent with interface portion and rifle head body; Also can be suitable for as required other material.
Intermediate thread pipe has the sealing shroud that can simply remove, and intermediate thread pipe is initially the full deflated state of screw thread closely, and overcoat sealing shroud simultaneously can stretch, retract after removal sealing shroud, and bending certain angle.
Interface portion is connected with intermediate thread pipe, and being connected of intermediate thread pipe and rifle head body, and is that adhesive type is tightly connected.
Material for interface portion, intermediate thread pipe, rifle head body is plastics, and color is milky.
This rifle head is the disposable liquid-transfering gun coating gun head that is applicable to the rifle head of 200 μ l liquid-transfering gun specifications, design parameter:
Interface portion length 1-2cm, peristome internal diameter 5-6mm, external diameter 7-8mm, thickness 1-2mm, for inserting liquid-transfering gun; Intermediate thread pipe is retractable curved 90, length 5mm-1cm, intracavity diameter 3-4mm, external diameter 5-6mm; Rifle head body: taper length 3-4cm, conical upper chamber diameter 3-4mm, external diameter 4-6mm, thickness 1-2mm.
The material of interface portion, intermediate thread pipe, rifle head body is respectively PP, polyurethane material, PP.
Its using method of disposable liquid-transfering gun coating gun head, comprises the following steps and 1. chooses required rifle head, and liquid-transfering gun is inserted to upper interface portions, keeps closely connecting; 2. draw sample liquids to the amount needing with liquid-transfering gun, pipette amount of liquid and be no more than 200 μ l; 3. imbitition is dripped to plate media surface: 4. rifle head body tip is withstood on to plate lid inner face, allow rifle head intermediate thread pipe bending 90, allow rifle tilt simultaneously, at 45 ° with media surface, push-and-pull 3 times previously, and then rotary coating, can complete the even coating of bacterium liquid like this.5., after being coated with, liquid-transfering gun rifle head is discarded.
By using result to show: disposable coating gun head involved in the present invention has the liquid of moving and two kinds of functions of coating, easy and simple to handle, flexible, high temperature resistant, high pressure resistant, disposable, and time and labour saving, pollutes less, and consumption is large, Yong Huguang.
The above,, in order to technical scheme of the present invention to be described, is not intended to limit; Although the present invention is had been described in detail with reference to aforementioned content, those of ordinary skill in the art is to be understood that: it still can be modified to aforementioned recorded technical scheme and related content, or part technical characterictic is wherein equal to replacement; And these amendments or replacement do not make the essence of appropriate technical solution depart from the spirit and scope of embodiment of the present invention technical scheme.
Claims (9)
1. a disposable liquid-transfering gun coating gun head, is characterized in that this coating gun header structure composition comprises:
Interface portion, intermediate thread pipe, rifle head body.
2. the disposable liquid-transfering gun coating gun of one as claimed in claim 1 head, further possesses feature:
Interface portion, intermediate thread pipe, rifle head body three parts are suitable for connecting successively according to the order of interface portion, intermediate thread pipe, rifle head body, and interface portion is also applicable to be combined with the insertion section of pipettor;
This interface portion is truncated cone-shaped or cylindrical, and surface is smooth surface;
Interface portion, intermediate thread pipe, its making material using of rifle head body are transparent or translucent;
This intermediate thread pipe is made up of certain toughness and deformable material, and its material is consistent with interface portion and rifle head body, or is suitable for other material as required, the upper and lower homogeneous of its external diameter internal diameter;
This rifle head body is taper, and liquid outlet bore is thinner, thicker with intermediate thread pipe junction bore, smooth outer surface;
Have the sealing shroud that can simply remove, intermediate thread pipe is initially the full deflated state of screw thread closely, overcoat sealing shroud simultaneously, and after removal sealing shroud, intermediate thread pipe can be stretched, retract, and bending certain angle;
Interface portion is connected with intermediate thread pipe, and the connected mode of intermediate thread pipe and rifle head body, is that adhesive type is tightly connected, and screw-type is tightly connected, or with the screw-type of the O-ring seal three kinds of optional one of connected mode that are tightly connected.
3. the disposable liquid-transfering gun coating gun of one as claimed in claim 1 or 2 head, also possesses feature:
Material for interface portion, intermediate thread pipe, rifle head body is plastics, and color is colourless or possesses certain color.
4. the disposable liquid-transfering gun coating gun of one as claimed in claim 3 head, also possesses feature:
Be applicable to the design parameter of the disposable liquid-transfering gun coating gun head of the rifle head of 200 μ l liquid-transfering gun specifications:
Interface portion length 1-2cm, peristome internal diameter 5-6mm, external diameter 7-8mm, thickness 1-2mm, for inserting liquid-transfering gun;
Intermediate thread pipe is retractable curved 90, length 5mm-1cm, intracavity diameter 3-4mm, external diameter 5-6mm;
Rifle head body: taper length 3-4cm, conical upper chamber diameter 3-4mm, external diameter 4-6mm, thickness 1-2mm.
5. the disposable liquid-transfering gun coating gun of one as claimed in claim 3 head, also possesses feature:
Be applicable to the design parameter of the disposable liquid-transfering gun coating gun head of the rifle head of 1000 μ l liquid-transfering gun specifications:
Interface portion length 1-2cm, peristome internal diameter 5-6mm, external diameter 7-8mm, thickness 1-2mm, for inserting liquid-transfering gun;
Intermediate thread pipe is retractable curved 90, length 1-2cm, intracavity diameter 4-5mm, external diameter 6-7mm;
Rifle head body: taper, length 4-6cm, conical upper chamber diameter 5-6mm, external diameter 7-8mm, thickness 1-2mm.
6. the disposable liquid-transfering gun coating gun of the one as described in claim 4 or 5 head, also possesses feature:
The material of interface portion, intermediate thread pipe, rifle head body is respectively PP, polyurethane material, PP.
7. that has utilized the arbitrary described disposable liquid-transfering gun coating gun head of claim 1-7 moves a liquid using method, comprises the following steps:
1) choose the liquid-transfering gun of appropriate size, liquid-transfering gun is inserted to interface portion, keep closely connecting;
2) draw sample liquids to the amount needing with liquid-transfering gun, pipette amount of liquid and be no more than the amount of liquid that liquid-transfering gun specification can be held;
3) utilize rifle head, imbitition dripped to plate media surface:
4) rifle head body tip is withstood on to plate lid inner face, allows the bending of rifle head intermediate thread part, allow rifle tilt simultaneously, angled with media surface, after push-and-pull for several times, and then rotary coating, completes the even coating of liquid;
5), after being coated with, liquid-transfering gun rifle head is discarded.
8. move as claimed in claim 7 liquid coating process, it is characterized in that:
The operation of abovementioned steps (4) is specially: rifle head body tip is withstood on to plate lid inner face, allow rifle head intermediate thread part bending 90, allow rifle tilt simultaneously, become 30-45 ° with media surface, push-and-pull 2-3 time previously, and then rotary coating, complete the even coating of bacterium liquid.
9. as described in claim 7-8, move liquid coating process, it is characterized in that:
The liquid-transfering gun specification of selected cooperation can be two kinds of specifications of 200 μ l or 1000 μ l, also can choose other common specification liquid-transfering guns.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410207441.9A CN103990503B (en) | 2014-05-16 | 2014-05-16 | Disposable liquid-transfering gun coating gun head and using method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410207441.9A CN103990503B (en) | 2014-05-16 | 2014-05-16 | Disposable liquid-transfering gun coating gun head and using method thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103990503A true CN103990503A (en) | 2014-08-20 |
| CN103990503B CN103990503B (en) | 2015-09-09 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201410207441.9A Expired - Fee Related CN103990503B (en) | 2014-05-16 | 2014-05-16 | Disposable liquid-transfering gun coating gun head and using method thereof |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105907640A (en) * | 2015-02-20 | 2016-08-31 | 精工爱普生株式会社 | Container, liquid storing member, cartridge set, and method of manufacturing liquid storing member |
| CN107325961A (en) * | 2017-06-23 | 2017-11-07 | 湖北省农业科学院果树茶叶研究所 | Suction head type bacterium apparatus for coating |
| CN108395982A (en) * | 2018-05-30 | 2018-08-14 | 厦门市科环海洋生物科技有限公司 | A kind of bacterium colony spraying device |
| CN109499637A (en) * | 2019-01-27 | 2019-03-22 | 刘晓 | A kind of bent angle liquid relief plastics pipette tips |
| CN109777727A (en) * | 2019-03-11 | 2019-05-21 | 江西农业大学 | A kind of portable microbial suspension coating pipette tips and its design method |
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Patent Citations (7)
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| GB738927A (en) * | 1952-09-26 | 1955-10-19 | Andre Savary | Improvements in or relating to dropper closures |
| WO1999006149A1 (en) * | 1997-08-01 | 1999-02-11 | Aalto Scientific, Ltd. | Pipetting devices preloaded with standardized control sample materials |
| US6599755B1 (en) * | 1999-04-27 | 2003-07-29 | Basf Aktiengesellschaft | Method and device for applying small quantities of liquid |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105907640A (en) * | 2015-02-20 | 2016-08-31 | 精工爱普生株式会社 | Container, liquid storing member, cartridge set, and method of manufacturing liquid storing member |
| CN107325961A (en) * | 2017-06-23 | 2017-11-07 | 湖北省农业科学院果树茶叶研究所 | Suction head type bacterium apparatus for coating |
| CN108395982A (en) * | 2018-05-30 | 2018-08-14 | 厦门市科环海洋生物科技有限公司 | A kind of bacterium colony spraying device |
| CN108395982B (en) * | 2018-05-30 | 2024-04-23 | 厦门市科环海洋生物科技有限公司 | Colony spraying device |
| CN109499637A (en) * | 2019-01-27 | 2019-03-22 | 刘晓 | A kind of bent angle liquid relief plastics pipette tips |
| CN109777727A (en) * | 2019-03-11 | 2019-05-21 | 江西农业大学 | A kind of portable microbial suspension coating pipette tips and its design method |
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| Publication number | Publication date |
|---|---|
| CN103990503B (en) | 2015-09-09 |
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Granted publication date: 20150909 Termination date: 20160516 |