CN107325961A - Suction head type bacterium apparatus for coating - Google Patents
Suction head type bacterium apparatus for coating Download PDFInfo
- Publication number
- CN107325961A CN107325961A CN201710486305.1A CN201710486305A CN107325961A CN 107325961 A CN107325961 A CN 107325961A CN 201710486305 A CN201710486305 A CN 201710486305A CN 107325961 A CN107325961 A CN 107325961A
- Authority
- CN
- China
- Prior art keywords
- coating
- suction nozzle
- head type
- spreading rod
- hole seat
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000000576 coating method Methods 0.000 title claims abstract description 62
- 239000011248 coating agent Substances 0.000 title claims abstract description 60
- 241000894006 Bacteria Species 0.000 title claims abstract description 36
- 238000005213 imbibition Methods 0.000 abstract description 2
- 238000002474 experimental method Methods 0.000 description 6
- 239000007788 liquid Substances 0.000 description 5
- 238000006243 chemical reaction Methods 0.000 description 2
- 239000004744 fabric Substances 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 230000001954 sterilising effect Effects 0.000 description 2
- 230000010310 bacterial transformation Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000000969 carrier Substances 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 238000004064 recycling Methods 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/04—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Molecular Biology (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention discloses a kind of suction head type bacterium apparatus for coating, including liquid-transfering gun, spreading rod, the hole seat for placing spreading rod, the outer box for accommodating spreading rod and hole seat；The spreading rod is made up of cone coating suction nozzle and spiral block；The coating suction nozzle matches with liquid-transfering gun, and the wide mouth part of coating suction nozzle is provided with external screw thread, and annular groove is provided with the end bore of coating suction nozzle；The Handheld Division that the spiral block connects by fitting portion and with fitting portion is constituted, and the inwall of fitting portion is provided with the internal thread matched with the external screw thread of the wide mouth part of coating suction nozzle；The hole seat is provided with for placing the suction nozzle hole of coating suction nozzle and placing the block hole of spiral block.The present invention set on liquid-transfering gun suction nozzle can mechanical deflection annular groove, and be equipped with the spiral block that matches with it, it is provided simultaneously with the function that imbibition and bacterium are coated with, be greatly improved conventional efficient and save experimental cost.
Description
Technical field
The present invention relates to bacterium apparatus for coating technical field, and in particular to be a kind of suction head type bacterium apparatus for coating.
Background technology
Spreading rod is biology laboratory common tool, is used for engineering bacteria vector construction conversion coated plate；Suction pipette head
It is liquid relief consumptive material the most commonly used during life science is tested.The bacterium spreading rod of domestic market sales is broadly divided into two kinds：
(a) disposable hand-held plastic-coated rod；(b) the recycling spreading rod that metal or glass make.The former additional package and sheet
Body easily causes energy waste, and the latter needs reserved instrument cooling or the repeatedly time of bacterium sterilization in use.Two
The common issue that person is present is, is unfavorable for the Bacterial Transformation experiment of once high-volume (more than 10), while adding experiment
The complexity that material prepares, influences conventional efficient.
The content of the invention
The purpose of the present invention be in view of the above-mentioned problems of the prior art, provide one kind can high efficiency carry out high-volume it is thin
Bacterium transformation experiment, and the suction head type bacterium apparatus for coating of experimental cost can be reduced.
To achieve the above object, the present invention is adopted the following technical scheme that：
Suction head type bacterium apparatus for coating, including liquid-transfering gun, spreading rod, for place spreading rod hole seat, for accommodate apply
The outer box of cloth rod and hole seat；The spreading rod is made up of cone coating suction nozzle and spiral block；The coating suction nozzle and liquid relief
Rifle matches, and the wide mouth part of coating suction nozzle is provided with external screw thread, annular is provided with the end bore of coating suction nozzle recessed
Groove；The Handheld Division that the spiral block connects by fitting portion and with fitting portion is constituted, and the inwall of fitting portion is provided with and is coated with
The internal thread that the external screw thread of the wide mouth part of suction nozzle matches；The hole seat is provided with the suction nozzle hole for placing coating suction nozzle and put
Put the block hole of spiral block.
Further, the side wall of the hole seat is provided with vertical groove；The interior of the box body of the outer box is provided with
Vertical projections；The vertical groove of the hole seat and the vertical projections of outer box match, and so can be placed in during moving, hole seat
Slid in outer box.
Preferably, the height of the outer box is 85~90mm.
Preferably, a diameter of 6mm of the coating suction nozzle, length is 55~60mm.
Preferably, the distance of end bore of the annular groove on the coating suction nozzle with being coated with suction nozzle is
15mm。
Preferably, the height of the spiral block is 20~30mm；The height of the fitting portion of the spiral block
For 10~15mm.
The invention has the advantages that：
1st, relative to existing bacterium spreading rod, the present invention integrates the use function of liquid-transfering gun suction nozzle, in liquid-transfering gun suction nozzle
Upper setting can mechanical deflection annular groove, and be equipped with the spiral block that matches with it, it is provided simultaneously with imbibition and bacterium
The function of coating, moreover, its volume is only existing trigonometric expression bacterium spreading rod 1/10~1/5, greatlys save raw materials for production,
Experimental cost is reduced, and causes coating suction nozzle function diversification.
2nd, the present invention has micro liquid concurrently and draws function in itself, can be stored in as common suction nozzle in the seat of hole, before experiment
Can centralized high voltage sterilizing, shorten experiment appliance time, also simplify disposable a large amount of bacterium coating process, greatly improve
Conventional efficient.
When the 3rd, being tested using the present invention, bacterium solution can be carried out by liquid-transfering gun set with the coating suction nozzle of spreading rod
Piping and druming is allowed to uniform, then, lives to be coated with the wide mouth part of suction nozzle using screw lid cap sleeve, it is taken out from container, and point touches coating
Annular groove on suction nozzle, makes its mechanical deflection to proper angle (60 °~90 °), you can to carry out bacterium coating, be once coated with
Former suction nozzle is abandoned after finishing, operating process easily and fast, and can be prevented effectively from cross pollution, in a large amount of opertions engineering bacterium carriers
It is with the obvious advantage during structure conversion coated plate, greatly improve conventional efficient.
Brief description of the drawings
Fig. 1 is the structural representation of spreading rod in suction head type bacterium apparatus for coating of the present invention；
Fig. 2 is the structural representation of suction head type bacterium apparatus for coating mesopore of the present invention seat；
Fig. 3 is the structural representation of outer box in suction head type bacterium apparatus for coating of the present invention.
In figure：Spreading rod 1 is (wherein：It is coated with suction nozzle 11, spiral block 12；Suction nozzle 11 is coated with again including wide mouth part 111, end
Port footpath 112, annular groove 113；Spiral block 12 is again including fitting portion 121, Handheld Division 122), hole seat 2 (wherein：Suction nozzle hole
21st, block hole 22, vertical groove 23), outer box 3 (wherein：Box body 31, vertical projections 32).
Embodiment
The invention will be further described with specific embodiment below in conjunction with the accompanying drawings.
As shown in Figure 1, Figure 2 and Figure 3, suction head type bacterium apparatus for coating of the present invention, including liquid-transfering gun, spreading rod 1, for putting
The hole seat 2 of spreading rod 1, the outer box 3 for accommodating spreading rod 1 and hole seat 2 are put, wherein, the height of outer box 3 is 85~90mm.
As shown in figure 1, cone coating suction nozzle 11 and height that the spreading rod 1 is 60mm by a diameter of 6mm, length are
20mm spiral block 12 is constituted；The coating suction nozzle 11 matches with liquid-transfering gun, and the wide mouth part 111 of coating suction nozzle 11 is set
There are 2~3 circle external screw threads, be provided with annular groove 113 at the 15mm of the end bore 112 of coating suction nozzle 11, the annular is recessed
Being provided with for groove 113 is beneficial to bottoming out coating suction nozzle 11 into " L " shape for being bent to 60 °~90 °；The spiral block 12 is by being highly
10mm fitting portion 121 and the Handheld Division 122 connected with fitting portion 121 are constituted, and the inwall of fitting portion 121 is provided with and is coated with
The internal thread that the external screw thread of the wide mouth part 111 of suction nozzle 11 matches.
As shown in Fig. 2 the hole seat 2 is provided with the suction nozzle hole 21 for placing coating suction nozzle 11 and places spiral block 12
Block hole 22；The side wall of the hole seat 2 is provided with vertical groove 23, wherein, hole seat 2 is 88+2 types, and it can place 88 amounts
Coating suction nozzle 11 and two spiral blocks 12 of the journey for 200 μ L.
As shown in figure 3, the interior of the box body 31 of the outer box 3 is provided with vertical projections 32；Hole seat 2 it is vertical
Groove 23 and the vertical projections 32 of outer box 3 match.
Bacterium coating is carried out using suction head type bacterium apparatus for coating of the present invention, comprised the following steps：
1) 88+2 type holes seat 2 is put into the box body 31 of outer box 3, coating suction nozzle 11 is positioned in the suction nozzle hole 21 of hole seat 2,
Spiral block 12 is positioned in the block hole 22 of hole seat 2, then, the autoclaving before being tested；
2) range is placed on to the wide mouth part 111 of coating suction nozzle 11 on superclean bench for 200 μ L liquid-transfering gun, bacterium is blown and beaten
Liquid is allowed to uniform, and pipettes the bacterium solution into culture plate, abandons former coating suction nozzle 11；
3) take spiral block 12 to be enclosed on the wide mouth part 111 of new coating suction nozzle 11, it is taken out from hole seat 2, point, which is touched, to be applied
On cloth suction nozzle 11 can mechanical deflection 113 to 60 ° of annular groove~90 °, you can to carry out bacterium coating, after bacterium coating terminates
Abandon former coating suction nozzle 11；
4) repeat step 1)~3) carry out next time bacterium take liquid and coating process, until all experimentss complete.
Other parts not being described in detail belong to prior art.
Claims (6)
1. suction head type bacterium apparatus for coating, including liquid-transfering gun, spreading rod (1), the hole seat (2) for placing spreading rod (1), it is used for
Accommodate the outer box (3) of spreading rod (1) and hole seat (2), it is characterised in that：The spreading rod (1) is by cone coating suction nozzle (11)
With spiral block (12) composition；The coating suction nozzle (11) matches with liquid-transfering gun, and the wide mouth part (111) of coating suction nozzle (11)
External screw thread is provided with, end bore (112) place close to coating suction nozzle (11) is provided with annular groove (113)；The screw lid
The Handheld Division (122) that cap (12) connects by fitting portion (121) and with fitting portion (121) is constituted, and the inwall of fitting portion (121) is set
It is equipped with the internal thread matched with the external screw thread of the wide mouth part (111) of coating suction nozzle (11)；The hole seat (2) is provided with for putting
Put the suction nozzle hole (21) of coating suction nozzle (11) and place the block hole (22) of spiral block (12).
2. suction head type bacterium apparatus for coating according to claim 1, it is characterised in that：The side wall of the hole seat (2) is set
There is vertical groove (23)；The interior of the box body (31) of the outer box (3) is provided with vertical projections (32)；The hole seat (2)
Vertical groove (23) and the vertical projections (32) of outer box (3) match.
3. suction head type bacterium apparatus for coating according to claim 1 or 2, it is characterised in that：The height of the outer box (3) is
85~90mm.
4. suction head type bacterium apparatus for coating according to claim 3, it is characterised in that：The diameter of the coating suction nozzle (11)
For 6mm, length is 55~60mm.
5. suction head type bacterium apparatus for coating according to claim 4, it is characterised in that：Ring on the coating suction nozzle (11)
The distance of connected in star (113) and the end bore (112) of coating suction nozzle (11) is 15mm.
6. suction head type bacterium apparatus for coating according to claim 5, it is characterised in that：The height of the spiral block (12)
For 20~30mm；The height of the fitting portion (121) of the spiral block (12) is 10~15mm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710486305.1A CN107325961A (en) | 2017-06-23 | 2017-06-23 | Suction head type bacterium apparatus for coating |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710486305.1A CN107325961A (en) | 2017-06-23 | 2017-06-23 | Suction head type bacterium apparatus for coating |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107325961A true CN107325961A (en) | 2017-11-07 |
Family
ID=60194793
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710486305.1A Pending CN107325961A (en) | 2017-06-23 | 2017-06-23 | Suction head type bacterium apparatus for coating |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107325961A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109251850A (en) * | 2018-11-13 | 2019-01-22 | 无锡市疾病预防控制中心 | A kind of automatic L stick of hand-held |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2005224194A (en) * | 2004-02-13 | 2005-08-25 | Japan Tissue Engineering:Kk | Pipette pipe and pipette apparatus |
| CN202671538U (en) * | 2012-06-28 | 2013-01-16 | 庄菁 | Bacterium coating rod |
| CN103990503A (en) * | 2014-05-16 | 2014-08-20 | 临沂大学 | Disposable pipette coating tip and using method thereof |
| CN203842600U (en) * | 2014-05-16 | 2014-09-24 | 临沂大学 | One-time pipetting gun coating head |
-
2017
- 2017-06-23 CN CN201710486305.1A patent/CN107325961A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2005224194A (en) * | 2004-02-13 | 2005-08-25 | Japan Tissue Engineering:Kk | Pipette pipe and pipette apparatus |
| CN202671538U (en) * | 2012-06-28 | 2013-01-16 | 庄菁 | Bacterium coating rod |
| CN103990503A (en) * | 2014-05-16 | 2014-08-20 | 临沂大学 | Disposable pipette coating tip and using method thereof |
| CN203842600U (en) * | 2014-05-16 | 2014-09-24 | 临沂大学 | One-time pipetting gun coating head |
Non-Patent Citations (1)
| Title |
|---|
| 钱君: "《基因探秘》", 上海人民出版社, pages: 69 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109251850A (en) * | 2018-11-13 | 2019-01-22 | 无锡市疾病预防控制中心 | A kind of automatic L stick of hand-held |
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|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20171107 |
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| RJ01 | Rejection of invention patent application after publication |