CN1039844A - From silkworm chrysalis, extract the method for amino acid composite liquid - Google Patents
From silkworm chrysalis, extract the method for amino acid composite liquid Download PDFInfo
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- CN1039844A CN1039844A CN88109976A CN88109976A CN1039844A CN 1039844 A CN1039844 A CN 1039844A CN 88109976 A CN88109976 A CN 88109976A CN 88109976 A CN88109976 A CN 88109976A CN 1039844 A CN1039844 A CN 1039844A
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- amino acid
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Abstract
A kind of is raw material with the silkworm chrysalis, add neutral protease, generate the method for amino acid composite liquid behind enzyme digestion reaction, its main flow process is: the flushing silkworm chrysalis to neutral, grind, go to add water behind the intestines and stir into pasty state, add an amount of neutral protease, 50 ℃ of temperature, enzymolysis is 5 hours under the condition of normal pressure, the residue after the filtration, enzymolysis repeatedly under similarity condition can extract edible amino acid composite liquid in higher efficiency.Abundant raw material of the present invention, technology is simple, and cost is low, and the product amino acid composite liquid can be used for preparing various beverages.
Description
The present invention relates to adopt proteolytic enzyme from silkworm chrysalis, to extract the method for amino acid composite liquid.
Acid, the proteic methods of basic hydrolysis pupa of adopting need to add strong acid, highly basic more in present the production in the reaction, not only production process complexity, and the generation three wastes, contaminate environment.The someone utilizes various protease hydrolyzed pupa albumen in recent years, produces amino acid composite liquid.Liaoning Normal University submitted " method that is prepared amino acid composite liquid by cocoon chrysalis " application for a patent for invention, (CN86106166A, application for a patent for invention prospectus version on October 7th, 87) to Patent Office of the People's Republic of China on September 8th, 86.This method is to utilize wooden pawl protease hydrolyzed tussah silkworm chrysalis protein to produce amino acid composite liquid.This method production technique is simple, cost is low, the no three wastes produce.But the amino acid whose extraction yield of this method is lower, and economic benefit is not high enough.
The objective of the invention is: provide a kind of employing new raw material, technology is simple, cost is low, do not produce the three wastes, the processing method that amino acid whose extraction yield improves relatively in the silkworm chrysalis, the product that makes does not have fishy smell, contains original amino acid composition, is suitable for eating.
Technical scheme of the present invention is: produce amino acid composite liquid with the neutral protease enzymolysis silkworm chrysalis.
Feature of the present invention is: after silkworm chrysalis earlier removes intestines with attrition process, and neutral protease enzymolysis under the following conditions:
1, enzyme amount: dried pupa amount=1: 200～300(weight ratio)
(vigor of enzyme is in 1,000,000 units/gram)
2, dried pupa amount: water=1: the 5(weight ratio) (add water and be stirred to pasty state)
3, hydrolysis temperature is 37 ℃～55 ℃
4, enzymolysis time is 3～8 hours
5, the pH value of reaction solution is 7～8 during enzymolysis.
To the residue that filters for the first time, enzymolysis once more under above-mentioned similarity condition is advisable with 2～3 times.
The optimum enzymolysis condition of present method is:
1, enzyme amount: dried pupa amount=1: the 200(weight ratio)
(unit of enzyme is in 1,000,000 units/gram)
2, dried pupa amount: water=1: the 5(weight ratio) furnishing pasty state
3, hydrolysis temperature: 50 ℃～55 ℃
4, enzymolysis time: 5 hours
5, enzyme digestion reaction liquid pH value=7～7.5
The Production Flow Chart of producing amino acid composite liquid with neutral protease enzymolysis pupa albumen is as follows:
Water flushing silkworm chrysalis to neutral (daylight shines, baking)/(most probably doing) grinds (sieving)/((as far as possible keeping the complete of the interior intestines of silkworm chrysalis body)) and adds water and stir into pasty state (pump)/() reactor (adding neutral protease)/(heating) enzymolysis (filtration)/() rinsing residue → filtration (being cooled to room temperature)/(adding gac) and take off (the filtration)/() amino acid composite liquid of distinguishing the flavor of → leave standstill.
In the production process of the present invention, removing raw meat and enzyme-added enzymolysis except that intestines is the key link of this law.Therefore, following several links is described in detail: 1, first procedure, extremely neutral with the flushing with clean water silkworm chrysalis, because silk cocoon is in silk reeling process, to use the thermokalite water treatment, silkworm chrysalis itself has been alkalescence, therefore feeding intake before with flushing with clean water to neutral, control the important step of pH value after being in the reaction process.To wash and shine, be baked to eighty per cant to the neutral silkworm chrysalis with daylight and do, this condition is very important, is not to rely on chemical process because this law is removed silkworm chrysalis fishy smell, but adopts the physical method that removes intestines; When silkworm chrysalis was in eighty per cant dried state, the intestines of silkworm chrysalis were hard, and in the process of grinding, silkworm chrysalis is broken, and that intestines are still preserved is complete, and after the sieve screening, intestines are promptly separable.The amino acid composite liquid fishy smell that makes is removed substantially, gac in addition again, and food removal agent of raw meat smell such as tealeaves, yellow rice wine, tangerine peel, fishy smell can all be removed.
2, in process of production, the enzyme amount that adds directly influences the effect of silkworm chrysalis enzymolyzing, the neutral protease that the present invention adopts and the ratio of silkworm chrysalis are with 1: 200～300(weight ratio) be advisable, and be the best (vigor of enzyme is with 1,000,000 units/gram) in 1: 200.
3, enzymolysis time: neutral protease needs the regular hour when the enzymolysis silkworm chrysalis protein, under reaction conditions of the present invention, through 5～8 hours reaction, can make amino acid composite liquid, filter the remaining residue in back, repeat enzyme-added, add water, stirring, enzymolysis again, its reason is a protein digestion when being amino acid, and the amount of amino acid of enzymolysis approximately is 50% of a protein content for the first time, and its residue is enzymolysis once more, but in the enzymolysis residue 50% of protein content, the rest may be inferred again.In order to make full use of raw material, to increase economic efficiency, enzymolysis time of the present invention was got 5 hours, behind the enzymolysis 2～3 times, can extract more than 50% of protein content.
4, hydrolysis temperature: among the present invention, the required temperature of neutral protease enzymolysis silkworm chrysalis is 37 ℃～55 ℃.In fact, neutral protease is in the time of 37 ℃, and the time that enzyme activity continues is the longest, during still owing to 37 ℃, and also suitable various microorganism growth, therefore, reaction solution is apt to deteriorate.So the present invention adopts 50 ℃ of these corresponding higher temperature, accelerate the silkworm chrysalis enzymolyzing process, solve the problem that the reaction solution souring is fouled.
The present invention adopts resourceful mulberry silkworm chrysalis to make raw material, and production technique, equipment are simple, and protein digestion efficient height is the method that a high-level efficiency is utilized the silkworm resource.
Embodiment:
Bright silkworm chrysalis is because in the alkaline purification of silk reeling process process, therefore be alkalescence, need to use the clear water thorough washing, to neutral, after water control done, when fine day, dry, use oven for drying during bad weather, but all GPRS is most probably done with the sun, like this when grinding, because intestines are crisp firmly and not, can be kept perfectly substantially, can remove through 20 mesh sieves.
Get 20 kilograms through the dried silkworm chrysalis of past intestines ground powdery, the tap water that adds the process purifying treatment is (in the technological process below, used clear water is through purifying treatment) 100 kilograms, the furnishing pasty state, squeeze in the reactor with beverage pump, in the reactor heating, start agitator, make slurries be warming up to 50 ℃～55 ℃, add neutral protease 100 grams (vigor of enzyme is 1,000,000 units/gram) this moment, keep reaction 5 hours (during this period of time can adopt intermittently and stir) with this understanding, be chilled to room temperature then, filter (using woven dacron) with whizzer (3000～4000 rev/mins), residue is integrated with in the filtrate with clear water washing back centrifuging.Residue adds clear water again, and the furnishing pasty state is squeezed in the reactor with beverage pump, adds proteolytic enzyme 50 grams, repeats above process.
Gained filtrate adds 80% sodium sulphite 200 milliliters (100 gram samples add 1 milliliter) in order to the precipitation metallic element.Add gac 40 grams (add-on is 0.2% of a raw material, takes off flavor in order to decolouring) simultaneously, under 55 ℃～60 ℃ temperature, decoloured 30 minutes, and intermittently stir.Cooled and filtered adds gac 40 grams (flavor is taken off in decolouring) again in the filtrate, still decoloured 30 minutes under 55 ℃～60 ℃ temperature, and intermittently stir cooled and filtered.Filtrate is 0 ℃ of left and right sides standing over night, next day is centrifuging again, add 1 kilogram of yellow rice wine in the filtrate, Jasmine tea 50 grams, tangerine peel 50 grams boiled 10 minutes, after the cooling solution is inclined to, wash in 1～2 solution merging filtrate of residue with clear water, the process underpressure distillation is poured into through in the disinfectant stainless steel cask after boiling cooling in 10 minutes again, makes it become 10% amino acid composite liquid finished product again.
Claims (4)
1, a kind of method of from silkworm chrysalis, extracting amino acid composite liquid, it is characterized in that: neutral protease and silkworm chrysalis carry out repeatedly enzymolysis under the following conditions:
Enzyme amount: dried pupa amount=1: 200～300 (weight ratios)
Dried pupa amount: water=1: 5 (weight ratio)
Hydrolysis temperature: 37 ℃～55 ℃
Enzymolysis time: 3～8 hours
Reaction solution PH=7～8 during enzymolysis
The product amino acid composite liquid that makes goes deodorization method to be: silkworm chrysalis removes intestines, adds the food removal agent of raw meat smell.
2, amino acid composite liquid preparation method according to claim 1 is characterized in that: silkworm chrysalis washes to neutrality, grinds, sieves, and the silkworm chrysalis intestines are removed.
3, amino acid composite liquid preparation method according to claim 1 is characterized in that: add yellow rice wine, tealeaves, tangerine peel in the amino acid composite liquid that makes.
4, amino acid composite liquid preparation method according to claim 1 is characterized in that: the silkworm chrysalis residue after enzymolysis filters, add water, enzyme-added under similarity condition enzymolysis 2～3 times.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN88109976A CN1039844A (en) | 1988-10-14 | 1988-10-14 | From silkworm chrysalis, extract the method for amino acid composite liquid |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN88109976A CN1039844A (en) | 1988-10-14 | 1988-10-14 | From silkworm chrysalis, extract the method for amino acid composite liquid |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN1039844A true CN1039844A (en) | 1990-02-21 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN88109976A Pending CN1039844A (en) | 1988-10-14 | 1988-10-14 | From silkworm chrysalis, extract the method for amino acid composite liquid |
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| CN (1) | CN1039844A (en) |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1061973C (en) * | 1993-10-27 | 2001-02-14 | 唐孝 | Technology for extracting amino acid of animal and plant |
| CN101849605A (en) * | 2010-05-17 | 2010-10-06 | 湖州澳特丝生物科技有限公司 | Preparation method of silkworm pupa high zinc protein powder |
| CN101406291B (en) * | 2008-11-06 | 2012-04-04 | 牟一心 | Herbaceous plant nutritious beverage and method for producing the same |
| CN103190524A (en) * | 2013-04-02 | 2013-07-10 | 江苏科技大学 | Method for improving silkworm chrysalis protein functional characteristics using ultrasonic wave coordinated with enzymolysis technology and product and application thereof |
| CN104921240A (en) * | 2015-05-27 | 2015-09-23 | 毛庆云 | High-protein silkworm chrysalis and bovine bone protein beverage and preparation method thereof |
| CN107242420A (en) * | 2017-07-03 | 2017-10-13 | 潘剑锋 | A kind of naturally enriched polypeptide and amino acid beverage and preparation method thereof |
| CN108967758A (en) * | 2018-07-10 | 2018-12-11 | 天津科技大学 | A kind of low-sugar drink and preparation method thereof containing insect protein |
-
1988
- 1988-10-14 CN CN88109976A patent/CN1039844A/en active Pending
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1061973C (en) * | 1993-10-27 | 2001-02-14 | 唐孝 | Technology for extracting amino acid of animal and plant |
| CN101406291B (en) * | 2008-11-06 | 2012-04-04 | 牟一心 | Herbaceous plant nutritious beverage and method for producing the same |
| CN101849605A (en) * | 2010-05-17 | 2010-10-06 | 湖州澳特丝生物科技有限公司 | Preparation method of silkworm pupa high zinc protein powder |
| CN101849605B (en) * | 2010-05-17 | 2012-07-18 | 湖州澳特丝生物科技有限公司 | Preparation method of silkworm pupa high zinc protein powder |
| CN103190524A (en) * | 2013-04-02 | 2013-07-10 | 江苏科技大学 | Method for improving silkworm chrysalis protein functional characteristics using ultrasonic wave coordinated with enzymolysis technology and product and application thereof |
| CN103190524B (en) * | 2013-04-02 | 2014-06-04 | 江苏科技大学 | Method for improving silkworm chrysalis protein functional characteristics using ultrasonic wave coordinated with enzymolysis technology and product and application thereof |
| CN104921240A (en) * | 2015-05-27 | 2015-09-23 | 毛庆云 | High-protein silkworm chrysalis and bovine bone protein beverage and preparation method thereof |
| CN107242420A (en) * | 2017-07-03 | 2017-10-13 | 潘剑锋 | A kind of naturally enriched polypeptide and amino acid beverage and preparation method thereof |
| CN108967758A (en) * | 2018-07-10 | 2018-12-11 | 天津科技大学 | A kind of low-sugar drink and preparation method thereof containing insect protein |
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