CN103977232A - Lily extract having alcohol-relieving function and application thereof - Google Patents
Lily extract having alcohol-relieving function and application thereof Download PDFInfo
- Publication number
- CN103977232A CN103977232A CN201410246263.0A CN201410246263A CN103977232A CN 103977232 A CN103977232 A CN 103977232A CN 201410246263 A CN201410246263 A CN 201410246263A CN 103977232 A CN103977232 A CN 103977232A
- Authority
- CN
- China
- Prior art keywords
- extract
- bulbus lilii
- extraction
- ethanol
- dry
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Medicines Containing Plant Substances (AREA)
Abstract
The invention discloses a lily extract having an alcohol-relieving function. The lily extract is prepared from the following steps: a. extracting dried lily with 30-70% of methanol or ethanol for 2-3 times, wherein the amount of the methanol or ethanol is 4-6 times that of the lily, wherein each time lasts for 1-2 hours; combining extracting solutions of various times, vacuum-condensing and drying to obtain an alcohol extract; b. adding 15-20 water to the alcohol extract so that the alcohol extract is dissolved into suspension, extracting the suspension through 1-1.5 times of one of petroleum ether, chloroform, benzene and cyclohexyl ring for 1-5 times to obtain an extracting solution A; c. adding 1-1.5 times of ethyl acetate or butyl alcohol to the extracting solution A and extracting for 1-5 times to obtain an extracting solution B; condensing and drying the extracting solution B to obtain the lily extract. A mice drunk experiment shows that the prepared lily extract disclosed by the invention has a certain alcohol-reliving function; therefore, the lily extract can be used for preparing alcohol-reliving medicines or healthcare products.
Description
Technical field
The present invention relates to Chinese medicine extract and application thereof, be specifically related to a kind of Bulbus Lilii extract and application thereof with antialcoholism action.
Background technology
Wine and human lives are closely related, and China is a country with deep spirits culture inside information especially.Size happy event, party and red-letter day, wine has become indispensable beverage in people's daily life, even becomes some businessmans indispensable on conference table " sharp weapon ".But excessive consumption of alcohol all can produce certain harm to human health and society, as dizzy in nausea and vomiting, headache, memory decreases, alcoholic hepatitis, liver cirrhosis; Make trouble after drinking, disturb public order, drive when intoxicated etc.Therefore, exploitation one safely and effectively Medicament for Alcoholism tool be of great significance.
Tiger lily Lilium lancifolium Thunb., bulb is near wide spherical, and high approximately 3.5 centimetres, diameter 4-8 centimetre; Scale width egg shape, long 2.5-3 centimetre, wide 1.4-2.5 centimetre, white.The high 0.8-1.5 rice of stem, band purple fringe, tool white MIAOMAO.Capsule is long and narrow avette, long 3-4 centimetre.The florescence 7-8 month, the fruit phase 9-10 month.Produce the provinces and regions such as Jiangsu, Zhejiang, Anhui, Jiangxi, Hunan, Hubei, Guangxi, Sichuan, Qinghai, Tibet, Gansu, Shaanxi, Shanxi, Henan, Hebei, Shandong and Jilin.Under the spinney of raw hillside, meadow, by roadside or water, height above sea level 400-2500 rice.There is cultivation various places.Also there are distribution in Japan, Korea.Bulb rich in starch, edible, also can make medicinal [152 pages of 1980 " Chinese Plants will " the 14th volumes].
Bulbus Lilii Lilium brownii F. E. Brown var. viridulum Baker, originates in Hebei, Shanxi, Henan, Shaanxi, Hubei, Hunan, Jiangxi, Anhui and Zhejiang.Be born in the thick grass of hillside, under sparse woods, by gully, by rand or village, also have cultivation, height above sea level 300-920 rice.Bulb containing enriching starch, is a kind of famous and precious food, also does medicinally, has nourishing the lung to arrest cough, heat clearing away, calms the nerves and the effect [152 pages of 1980 " Chinese Plants will " the 14th volumes] such as diuresis.
Lilium tenuifolium Lilium pumilum D C., bulb is avette or conical, high 2.5-4.5 centimetre, diameter 2-3 centimetre; Scale square is circular or long avette, long 2-3.5 centimetre, wide 1-1.5 centimetre, white.The florescence 7-8 month, the fruit phase 9-10 month.Produce Hebei, Henan, Shanxi, Shaanxi, Ningxia, Shandong, Qinghai, Gansu, the Inner Mongol, Heilungkiang, Liaoning and Jilin.Raw patana or border, height above sea level 400-2600 rice.Also there are distribution in the Soviet Union, Korea, Mongolia.Bulb is starch-containing, edible, also can be used as medicine, and has the effects such as strengthening by means of tonics, eliminating phlegm and stopping cough, diuresis.Spend beauty, can cultivate for viewing and admiring, also, containing volatile oil, can extract for spice [147 pages of 1980 " Chinese Plants will " the 14th volumes].
From finding out above, main effect of the bulb of tiger lily, Bulbus Lilii, this three kind of plant of Lilium tenuifolium is nourishing YIN and moistening the lung, clearing away heart-fire for tranquillization.For deficiency of YIN cough caused by dryness, chronic cough hemoptysis, fidgets due to deficiency palpitation with fear, insomnia and dreamful sleep, absentminded.Do not have so far and specifically report that Bulbus Lilii extract has antialcoholism action.
Summary of the invention
Object of the present invention is just to provide a kind of Bulbus Lilii extract with antialcoholism action; Bulbus Lilii extract of the present invention is through mice drunk experiment demonstration, and the administration group mice drunk rate that gives extract of the present invention obviously reduces, and points out Bulbus Lilii extract of the present invention to have certain antialcoholism action.
The Bulbus Lilii extract with antialcoholism action of the present invention is to be prepared from by the method for following step:
A. 30%～70% the methanol of dry Bulbus Lilii doubly being measured with 4-6 or ethanol extraction 2-3 time extract 1-2 hour at every turn; Merge extracting solution each time, vacuum concentration, dry, obtain ethanol extract;
B. in ethanol extract, add 15-20 times of water and make it be dissolved into suspension, then one extraction in having encircled with 1-1.5 petroleum ether, chloroform, benzene or ring doubly 1-5 time, extract A obtained;
C. in extract A, add again ethyl acetate doubly of 1-1.5 or n-butanol extraction 1-5 time, obtain extract B; Extract B is concentrated, dry, obtain Bulbus Lilii extract.
Preferably, the described Bulbus Lilii extract with antialcoholism action is to be prepared from by following method:
A. 70% the ethanol extraction of dry Bulbus Lilii doubly being measured with 4-6 2 times extracts 1-2 hour at every turn; Merge 2 times extracting solution, vacuum concentration, dry, obtain ethanol extract;
B. in ethanol extract, add 15-20 times of water and make it be dissolved into suspension, then with 1-1.5 petroleum ether extraction doubly 1-5 time, obtain extract A;
C. in extract A, add again 1-1.5 ethyl acetate extraction doubly 1-5 time, obtain extract B; Extract B is concentrated, dry, obtain Bulbus Lilii extract.
Described Bulbus Lilii is liliaceous plant tiger lily Lilium lancifolium Thunb. or Bulbus Lilii Lilium brownii F. E. Brown var. viridulum Baker or Lilium tenuifolium Lilium pumilum D C..
Described antialcoholic drugs or health product contain Bulbus Lilii extract and the pharmaceutically acceptable carrier for the treatment of effective dose.
Described antialcoholic drugs or health product can be made into tablet, capsule, granule, powder, ointment, oral agents.
The prepared Bulbus Lilii extract of the present invention is through the demonstration of mice drunk experimental result, and the administration group mice drunk rate that gives extract of the present invention obviously reduces, and points out Bulbus Lilii extract of the present invention to have certain antialcoholism action.
Detailed description of the invention
Embodiment 1
Get dry Bulbus Lilii 1000g, the ethanol that is 70% by 5L volume fraction, heating and refluxing extraction, extraction time is 2 times, the time is 1h, merge extractive liquid,, vacuum concentration, dry 70% ethanol extraction that obtains Bulbus Lilii.
Embodiment 2
After the meat bulb of Bulbus Lilii is cleaned, dries or dried, get dry Bulbus Lilii 1000g, the ethanol that is 30% by 5L volume fraction, heating and refluxing extraction, extraction time is 2 times, the time is 1h, merge extractive liquid,, vacuum concentration, dry 30% ethanol extraction that obtains Bulbus Lilii.
Embodiment 3
After the meat bulb of Bulbus Lilii is cleaned, dries or dried, get dry Bulbus Lilii 1000g, the ethanol that is 50% by 5L volume fraction, heating and refluxing extraction, extraction time is 2 times, the time is 1h, merge extractive liquid,, vacuum concentration, dry 50% ethanol extraction that obtains Bulbus Lilii.
Embodiment 4
After the meat bulb of Bulbus Lilii is cleaned, dries or dried, get dry Bulbus Lilii 1000g, the methanol that is 70% by 5L volume fraction, heating and refluxing extraction, extraction time is 2 times, the time is 1h, merge extractive liquid,, vacuum concentration, dry 70% methanolic extract that obtains Bulbus Lilii.
Embodiment 5
Get the 70% ethanol extraction 100g that embodiment 1 prepares Bulbus Lilii, the 2L that adds water is dissolved into suspension.Use 2L petroleum ether extraction, re-extract 3 times, gained extract extracts by 2L ethyl acetate again, re-extract 3 times, combined ethyl acetate extract, concentrates, is drying to obtain the Bulbus Lilii extract with antialcoholism action.
Embodiment 6
Get the 70% ethanol extraction 100g that embodiment 1 prepares Bulbus Lilii, the 2L that adds water is dissolved into suspension.Use 2L petroleum ether extraction, re-extract 3 times, gained extract is used 2L chloroform extraction again, re-extract 3 times, combined chloroform extract, concentrates, is drying to obtain the Bulbus Lilii extract with antialcoholism action.
Embodiment 7
Get the 50% ethanol extraction 100g that embodiment 3 prepares Bulbus Lilii, the 2L that adds water is dissolved into suspension.Use 2L chloroform extraction, re-extract 3 times, gained extract extracts by 2L ethyl acetate again, re-extract 3 times, combined ethyl acetate extract, concentrates, is drying to obtain the Bulbus Lilii extract with antialcoholism action.
Embodiment 8
The 70% methanolic extract 100g that embodiment 4 is prepared, the 2L that adds water is dissolved into suspension.With the extraction of 2L cyclohexane extraction, re-extract 3 times, gained extract extracts by 2L ethyl acetate again, re-extract 3 times, and combined ethyl acetate extract, concentrates, is drying to obtain the Bulbus Lilii extract with antialcoholism action.
Embodiment 9
The 70% methanolic extract 100g that embodiment 4 is prepared, the 2L that adds water is dissolved into suspension.With the extraction of 2L benzene, re-extract 3 times, gained extract is used 2L n-butanol extraction again, and re-extract 3 times merges butanol extraction liquid, concentrates, is drying to obtain the Bulbus Lilii extract with antialcoholism action.
Embodiment 10
Mice drunk experiment
Get 200 of white mice, body weight is 18 ~ 20g approximately, is divided at random 10 groups, 20 every group, establishes and gives 1 group of 9 groups of the administration groups of embodiment 1 ~ 9 extract and the matched group of not administration.Each group gavage every day to wine 0.4 mL/ only.Matched group every day to wine before 30min, gavage 0.4 mL that feeds water, each administration group every day to wine before 30min, gastric infusion 0.4 mL(gets the extract that embodiment 1 ~ 9 prepares and uses respectively water dissolution), continuous 30 days.In the time of the 2nd, 5,10,15,20 days, mice is carried out to righting reflex experiment respectively, observe to after drinking in 1h mice there is the number of elements of righting reflex loss, righting reflex loss thinks that animal is drunk, when each righting reflex experiment, every mouse experiment 5 times, observed result, calculates its drunk rate.
The experiment of table 1 mice drunk
Shown by the experiment in upper table, the drunk rate of mice of matched group (give wine before do not give extract of the present invention) reaches 90%, and the drunk rate of administration group mice that gives extract of the present invention obviously reduces.The effect of relieving the effect of alcohol after further extract extracting and refining is further improved, as embodiment 5, first remove after a little liposoluble constituents with petroleum ether, then be extracted with ethyl acetate, the extract obtaining after the extraction effect of relieving the effect of alcohol is best.With the drunk rate of administration group mice of embodiment 5 extract administrations be only 5%, further the extract administration group mice drunk rate of extracting and refining is not that 41%(is as embodiment 1 extract group).Meanwhile, above data show, relieve the effect of alcohol effect than by the further better effects if of extraction of n-butyl alcohol with the extract of the further extracting and refining of ethyl acetate, and as embodiment 9 use n-butyl alcohol further extract, the drunk rate of mice is 25%.
Claims (4)
1. there is a Bulbus Lilii extract for antialcoholism action, it is characterized in that being prepared from by following method:
A. 30%～70% the methanol of dry Bulbus Lilii doubly being measured with 4-6 or ethanol extraction 2-3 time extract 1-2 hour at every turn; Merge extracting solution each time, vacuum concentration, dry, obtain ethanol extract;
B. in ethanol extract, add 15-20 times of water and make it be dissolved into suspension, then one extraction in having encircled with 1-1.5 petroleum ether, chloroform, benzene or ring doubly 1-5 time, extract A obtained;
C. in extract A, add again ethyl acetate doubly of 1-1.5 or n-butanol extraction 1-5 time, obtain extract B; Extract B is concentrated, dry, obtain Bulbus Lilii extract.
2. a kind of Bulbus Lilii extract with antialcoholism action according to claim 1, it is characterized in that being prepared from by following method:
A. 70% the ethanol extraction of dry Bulbus Lilii doubly being measured with 4-6 2 times extracts 1-2 hour at every turn; Merge 2 times extracting solution, vacuum concentration, dry, obtain ethanol extract;
B. in ethanol extract, add 15-20 times of water and make it be dissolved into suspension, then with 1-1.5 petroleum ether extraction doubly 1-5 time, obtain extract A;
C. in extract A, add again 1-1.5 ethyl acetate extraction doubly 1-5 time, obtain extract B; Extract B is concentrated, dry, obtain Bulbus Lilii extract.
3. the Bulbus Lilii extract with antialcoholism action according to claim 1 and 2, is characterized in that: described Bulbus Lilii is liliaceous plant tiger lily Lilium lancifolium Thunb. or Bulbus Lilii Lilium brownii F. E. Brown var. viridulum Baker or Lilium tenuifolium Lilium pumilum D C..
4. the Bulbus Lilii extract with antialcoholism action described in claim 1-2 is in the application of preparing in antialcoholic drugs or health product.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410246263.0A CN103977232A (en) | 2014-06-05 | 2014-06-05 | Lily extract having alcohol-relieving function and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410246263.0A CN103977232A (en) | 2014-06-05 | 2014-06-05 | Lily extract having alcohol-relieving function and application thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN103977232A true CN103977232A (en) | 2014-08-13 |
Family
ID=51269559
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410246263.0A Pending CN103977232A (en) | 2014-06-05 | 2014-06-05 | Lily extract having alcohol-relieving function and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103977232A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113367341A (en) * | 2021-06-29 | 2021-09-10 | 华纳盛世(武汉)生物科技有限公司 | Composition for soothing nerves and improving sleep and preparation method thereof |
-
2014
- 2014-06-05 CN CN201410246263.0A patent/CN103977232A/en active Pending
Non-Patent Citations (2)
| Title |
|---|
| 小梧: "10种护肝蔬菜 百合最防肝硬化", 《农产品加工》 * |
| 李林等: "中药百合有效部位的药效学筛选", 《南京中医药大学学报》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113367341A (en) * | 2021-06-29 | 2021-09-10 | 华纳盛世(武汉)生物科技有限公司 | Composition for soothing nerves and improving sleep and preparation method thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Dong et al. | Cordyceps industry in China | |
| CN103316096A (en) | General flavone extract of seeds of nigella damascena l., nigella sativa l. or nigella glandulifera freyn et sint., and preparation method and use thereof | |
| CN103054008A (en) | Lucid ganoderma sleep-aiding capsule and preparation method thereof | |
| CN104274502B (en) | A kind of extracting method of the efficient antiinflammatory action compound of euscaphis konishii, product | |
| CN103598422A (en) | Chinese herbal medicine feed addictive for river crabs | |
| CN104666934A (en) | Cordyceps sinensis compound lozenge and preparation method thereof | |
| CN102178721B (en) | Application of fiveleaf gynostemma herb suspension and extract to preparation of drug for treating and resisting depression | |
| CN107854519A (en) | A kind of perilla leaf oil and its application | |
| CN103211858A (en) | Si-Jun-Zi dropping pill preparation | |
| CN104971088A (en) | Tibetan artemisia capillaris extract and preparation method, drug composition and application thereof | |
| CN103977232A (en) | Lily extract having alcohol-relieving function and application thereof | |
| CN101301357B (en) | Use of schisandra chinensis extract in anti-depression medicament | |
| CN102764294A (en) | Cough relieving and sputum eliminating combination and preparation method thereof | |
| CN100515230C (en) | New medicinal use of eupatorium lindleyanun | |
| CN101785826A (en) | Thunberg fritillary flower micropowder preparation method | |
| CN105561093A (en) | Formulation for treating cough | |
| CN105030874A (en) | Preparation method of platycodon extract and extract and drink prepared by preparation method | |
| CN104042677A (en) | Use of Manglietia fruit extractive in preparation of orthopaedic anti-inflammatory and analgesic medicine | |
| CN103610843A (en) | Ampelopsis japonica extractive with effect of relieving alcohol effect or protecting liver as well as preparation method and application thereof | |
| CN107753906A (en) | It is a kind of that there is the medicinal liquor for alleviating pernio effect | |
| CN103263475A (en) | Traditional chinese medicine composition | |
| CN102764288A (en) | Application of luffa water spray for treating acute pharyngitis | |
| CN103263446A (en) | Dicranopteris pedata extract with gastric cancer resistant effect and preparation method thereof | |
| CN101721448A (en) | Preparation method of silybum marianum total flavonoid as active ingredient of swertia pseudochinensis-silybum marianum liver benefiting product | |
| TWI654985B (en) | A use of an extract of a formula including a fruit body of antrodia cinnamomea in the manufacture of a medicament for the treatment of hepatitis b |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20140813 |
|
| WD01 | Invention patent application deemed withdrawn after publication |