CN103969251A - Device and method for quickly detecting integrated chemical reaction - Google Patents
Device and method for quickly detecting integrated chemical reaction Download PDFInfo
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- CN103969251A CN103969251A CN201410185603.3A CN201410185603A CN103969251A CN 103969251 A CN103969251 A CN 103969251A CN 201410185603 A CN201410185603 A CN 201410185603A CN 103969251 A CN103969251 A CN 103969251A
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Abstract
The invention relates to a device and method for quickly detecting an integrated chemical reaction, and relates to the technical field of quick detection of food, drugs, cosmetics and the like. The detection device is a cylindrical or circular cone-shaped sealed cavity, and consists of a sealed cover (1), a pretreatment chamber (2), a reaction chamber (3), a filter pipe (4), a pretreatment reagent (5) and a reaction reagent (6), wherein the sealed cover (1) is arranged at the top of the pretreatment chamber (2); the pretreatment chamber (2) is vertically communicated with the reaction chamber (3) through the filter pipe (4); a microporous filter element or other filter material (4.1) is mounted inside the filter pipe (4); the pretreatment reagent (5) is placed in the treatment chamber (2) or other containers in advance; the reaction reagent (6) is placed in the reaction chamber (6) in advance, or is frozen and dried, or is prepared into tablets or pills, or is added from a side hole (3.1). The detection method comprises the following steps: putting a sample in the pretreatment chamber (2), or adding the pretreatment reagent (5), sealing the cover, shaking, centrifuging, reacting, observing or detecting the result. The sample pretreatment, filtering and reaction integration are realized, and the method is simple, convenient and practical; standardization is easily realized; the detection device is suitable for rapid field detection.
Description
Technical field
The present invention relates to a kind of integrated chemical reaction device for fast detecting and method thereof; Specifically, relate to integrated chemical reaction device for fast detecting and the method for fast detecting such as a kind of food, medicine and cosmetics, belong to rapid detection technical field.
Background technology
In rapid detection technical field such as food, medicine and cosmetics, developed and applied and comprised the multiple Fast Detection Technique such as chemistry, micro-, chromatogram, spectrum, immunity, biology, modal or traditional chemical detection method, mainly because chemical detection method specificity is good, cost is low, simple to operate, the domestic fast detecting method of chemistry of having set up a lot of kinds.Generally, the method needs sample pretreatment, filtration in actual application, react with particular agent again, at least need three above steps, thereby the convenience that operates is not strong, add that most reagent reagents have certain murder by poisoning or easy contaminated environment to human body, while particularly operating at the scene, above-mentioned defect is more obvious.In operation, be also difficult to standardization simultaneously.
In a word, there is certain defect in the fast detecting method of common chemistry, uses also inconvenient.
Through retrieval, the patent that relates to pick-up unit related to the present invention and method has:
1, the reaction tube reacting for immune detection
Publication number: CN201935917U; Application number: 201020692924.X; The applying date: 2010-12-31.
2, a kind of automation equipment for application of sample and reaction tube loading
Publication number: CN201974433U; Application number: 201120037569.7; The applying date: 2011-02-14.
3, reactor tube apparatus
Publication number: CN101072633; Application number: CN200580042036.5; The applying date: 2005-12-08.
4, a kind of nucleic acid amplification and detection reaction pipe
Publication number: CN103103118A; Application number: CN201110360350.5; The applying date: 2011-11-15.
5, gene magnification, hybridization and detect integrated reaction pipe cap
Publication number: CN1372003; Application number: CN01108218.6; The applying date: 2001-02-20.
6, in single reaction tube, carry out the method for a plurality of reactions
Publication number: CN102574090A; Application number: CN201080031874.3; The applying date: 2010-06-16.
7, a kind of reaction tube of controlling reaction process by temperature
Publication number: CN101787347A; Application number: CN200910264173.3; The applying date: 2009-12-30.
8, for the reaction tube of Full-automatic chemiluminescence immunoassay analysis meter
Publication number: CN102147357A; Application number: CN201110037129.6; The applying date: 2011-02-14.
9, a kind of reaction tube for loop-mediated isothermal amplification technique and using method thereof
Publication number: CN101712924A; Application number: CN200910213987.4; The applying date: 2009-12-18.
10, a kind of reaction tube that can realize airtight detection nucleic acid amplification product in same pipe
Publication number: CN101724553A; Application number: CN200910264065.6; The applying date: 2009-12-29.
Do not find the patented invention content identical with present patent application.
Summary of the invention
Object of the present invention is just to overcome the shortcoming and defect that prior art exists, and a kind of integrated chemical reaction device for fast detecting and method are provided.This device and method is easy to use, efficiency is high, practical, be easy to realize standardization; Be particularly suitable for the field quick detection such as food, medicine and cosmetics.Also be requirement and the embodiment of microminiaturized, the integrated and automation development new trend of analytical technology.
The object of the present invention is achieved like this:
The present invention adopts engineering plastics or the glass tube of acid and alkali-resistance and organic solvent, and the pre-service of sample, filtration, reaction are integrated in this device; This device is a cylinder or cone shape airtight cavity; By gland bonnet, pretreatment chamber, reaction chamber, strainer tube, pretreating reagent and reaction reagent, formed; Pretreatment chamber top arranges gland bonnet; Pretreatment chamber is communicated with by strainer tube up and down with reaction chamber; Micropore filter element or other filtering materials are installed in strainer tube; Pretreating reagent is placed in pretreatment chamber in advance, or puts in other container; Reaction reagent is placed in reaction chamber in advance; Or freeze drying, or make tablet, piller; Or add from reaction chamber side opening; Detection method is for to be placed in pretreatment chamber by sample, or adds pretreating reagent, capping, and jolting, centrifugal, reaction, observes or testing result.
Specifically, as accompanying drawing 1, accompanying drawing 2, pick-up unit of the present invention is a cylinder or cone shape airtight cavity; By gland bonnet (1), pretreatment chamber (2), reaction chamber (3), strainer tube (4), pretreating reagent (5) and reaction reagent (6), formed.
Its position and annexation are:
Pretreatment chamber (2) top arranges gland bonnet (1), and gland bonnet (1) is connected with pretreatment chamber (2) by screw thread or connection Jiao Qiao, plays sealing function;
Pretreatment chamber (2) is communicated with by strainer tube (4) up and down with reaction chamber (3); Micropore filter element or other filtering materials (4.1) are installed in strainer tube (4);
Pretreating reagent (5) is placed in pretreatment chamber (2) in advance, or puts in other container;
Reaction reagent (6) is placed in reaction chamber (3) in advance; Or add from side opening (3.1).
Detection method is for to be placed in pretreatment chamber (2) by sample, or adds pretreating reagent (5), capping, and jolting, centrifugal, reaction, observes or testing result.
Principle of work of the present invention is:
Reaction chamber (3) carries out the reacting environment of fast detecting for sample, during use, first open gland bonnet (1), appropriate sample is placed in to pretreatment chamber (2), pretreating reagent (5) liquid level of the inside is raise, surpass the upper top of strainer tube (4); With gland bonnet (1), seal; Jolting mixes, sample pretreatment; Centrifugal, the sample solution that surpasses the upper top of strainer tube (4) utilizes centrifugal force to pass through micropore filter element or other filtering materials (4.1) of strainer tube (4), collects reaction chamber (3), reacts with reaction reagent (6); The phenomenon judged result arriving according to the observation; Or utilize pertinent instruments as photometer etc. detects, obtain test findings.
The present invention has the following advantages and good effect:
1. sample pretreatment, filtration, reaction are integrated in plastics or glass reaction tube, have effectively simplified operation steps.
2. pretreating reagent is placed in pretreatment chamber in advance, its liquid level is lower than top on strainer tube, and when whole pick-up unit is inverted or is tilting its liquid level also lower than strainer tube on top, add that strainer tube top design becomes circular cone or dome shape, aperture is set, increase surface tension, so just effectively avoided pretreating reagent contaminated filter pipe filter core in storage and transportation, also avoided pretreating reagent to flow in reaction chamber by strainer tube.
3. in sample Jia Zhi pretreatment chamber, liquid level raises, and partially liq can flow to reaction tube from strainer tube by centrifugal, reacts with reaction reagent.
4. reaction reagent divides in advance and installs to reaction chamber, its liquid level is lower than bottom under strainer tube, and when whole pick-up unit is inverted or is tilting its liquid level also lower than strainer tube under bottom, add that strainer tube bottom is designed to circular cone or dome shape, aperture is set, increase surface tension, avoided reaction reagent in storage and transportation to enter in pretreatment chamber by strainer tube.
5. reaction reagent can freeze drying; Or make tablet or piller, to improve the term of validity of reagent; According to the difference of fast detecting method, can conveniently select the processing mode of above-mentioned various reaction reagents, make it to be applicable to various fast detecting methods.
6. according to the character of pretreating reagent and reaction reagent, above-mentioned gland bonnet, reaction tube, strainer tube all select the plastics of acid and alkali-resistance or organic solvent or glass to make, and have improved the adaptability of this device.
according to the characteristic of pretreating reagent, select the filtering material of acid and alkali-resistance or organic solvent, or use water, organic phase filter membrane, or use the filter core of various materials, further expand its adaptability.
When 8. reality detects, as long as sample is placed in to pretreatment chamber, sealing, jolting, centrifugal, reaction, can directly observe or testing result.Generally, above-mentioned all operations can just can complete in 1～5min, has improved detection speed.
In a word, because the present invention has realized sample pretreatment, filtration, reaction is integrated, easy to use, efficiency is high, practical; Be easy to realize standardization; Be particularly suitable for the field quick detection such as food, medicine and cosmetics.
Accompanying drawing explanation
Figure of description 1, accompanying drawing 2 are structural drawing of 2 different pick-up units of the present invention.Accompanying drawing 1 be applicable to only have 1 step reaction detection method, be applicable to multistep reaction reagent to be incorporated into the detection method of reaction chamber, and be applicable to have reacted Hou Cong pretreatment chamber in the first step and add again the 2nd step and above reaction reagent and enter by centrifugal the detection method that reaction chamber reacts; Accompanying drawing 2 is applicable to multistep reaction, and the 1st step reaction reagent is placed in reaction chamber in advance, and the 2nd step and above reaction reagent add from reaction chamber side opening.
Wherein:
1-gland bonnet;
2-pretreatment chamber;
3-reaction chamber,
3.1-side opening,
3.2-side opening gland bonnet;
4-strainer tube,
4.1-mistake filter core,
4.2-upper top aperture,
4.3-lower bottom part aperture;
5-pretreating reagent;
6-reaction reagent.
Embodiment
One, installation composition
As accompanying drawing 1, accompanying drawing 2, pick-up unit of the present invention is a cylinder or cone shape airtight cavity; Gland bonnet (1), pretreatment chamber (2), reaction chamber (3) connect from top to bottom successively;
Pretreatment chamber (2) top arranges gland bonnet (1), and gland bonnet (1) is connected with pretreatment chamber (2) by screw thread or connection Jiao Qiao, plays sealing function;
Pretreatment chamber (2) is communicated with by strainer tube (4) up and down with reaction chamber (3);
Reaction chamber (3) sidewall or have side opening (3.1), with side opening gland bonnet (3.2) sealing;
Micropore filter element or other filtering materials (4.1) are installed in strainer tube (4); Upper and lower tip designs becomes circular cone or dome shape, and center arranges aperture (4.2), (4.3);
Pretreating reagent (5) is placed in pretreatment chamber (2) in advance, or puts in other container;
Reaction reagent (6) is placed in reaction chamber (3) in advance; Or add from side opening (3.1).
Two, each component specification
1, gland bonnet (1)
Gland bonnet (1) is cylindrical plastic cover, by screw thread or connection Jiao Qiao, is connected with pretreatment chamber (2), plays the effect of sealing, by engineering plastics injection moulding (blowing), is formed.
2, pretreatment chamber (2)
Pretreatment chamber (2) is the airtight cavity of a right cylinder or cone, and external diameter is 2～50mm; Internal diameter is 1～48mm; Volume is 1～20ml; Sidewall has volume marking line; According to the characteristic of pretreating reagent, select the engineering plastics injection moulding (blowing) of various acid and alkali-resistances, organic solvent-resistant to form, also useable glass processes.
3, reaction chamber (3)
Reaction chamber (3) is the airtight cavity of a right cylinder or cone, and external diameter is 2～50mm; Internal diameter is 1～48mm; Volume is 1～20ml; Sidewall has volume marking line; Bottom design becomes point end shape, is beneficial to collecting precipitation and observations; According to the characteristic of reaction reagent, select the engineering plastics injection moulding (blowing) of various acid and alkali-resistances, organic solvent-resistant to form, also useable glass processes.
4, strainer tube (4)
Strainer tube (4) is a hollow circular cylinder, and external diameter is 1～10mm; Internal diameter is 0.5～8mm; Volume is 0.2～10ml; The micropore filter element that it is built-in or other filtering materials (4.1) aperture are 0.22～20um; Upper and lower side is all designed to circular cone or dome shape, and center arranges aperture (4.2) and aperture (4.3), and aperture is 0.5～2mm.
5, pretreating reagent (5)
Pretreating reagent (5) can be various organic solvents, acid-alkali salt, damping fluid and mixing thereof, and volume is 1～10ml; Be placed in advance pretreatment chamber (2); Its liquid level is lower than the upper top of strainer tube (4), and its liquid level, also lower than the upper top of strainer tube (4), guarantees that in storage and transportation, pretreating reagent (5) can not enter in reaction chamber (3) by strainer tube (4) when whole pick-up unit is inverted or is tilting.
6, reaction reagent (6)
Reaction reagent (6) is the reagent of the various chemical reactions of preparation, and volume is 0.5～10ml; Reaction reagent (6) divides in advance and installs in reaction chamber (3), its liquid level is lower than the lower bottom of strainer tube (4), and its liquid level, also lower than the lower bottom of strainer tube (4), guarantees that in storage and transportation, reaction reagent (6) can not enter in pretreatment chamber (2) by strainer tube (4) when whole pick-up unit is inverted or is tilting;
Reaction reagent (6) can freeze drying; Or make tablet or piller, be placed in reaction tube (2) bottom; Reach the object of extending the expiration date;
For there being the above detection method of 2 steps reactions, relate to the 2nd step and later reaction reagent (6) can add from side opening (3.1); Also can add in pretreatment chamber (2) and enter reaction chamber (3) by centrifugal method.
Three, detection method
1, accompanying drawing 1 is applicable to only have the detection method of 1 step reaction: while using this device to detect, only appropriate sample need be placed in to pretreatment chamber (2), and capping, jolting, centrifugal, reaction, directly observes or testing result.
2, accompanying drawing 1 is applicable to multistep reaction reagent to be incorporated into the detection method of reaction tube: multistep reaction reagent is incorporated in reaction chamber (3) in advance, while using this device to detect, only appropriate sample need be placed in to pretreatment chamber (2), capping, jolting, centrifugal, reaction, directly observes or testing result.
3, accompanying drawing 1 is applicable to have reacted that Hou Cong pretreatment chamber adds the 2nd step and above reaction reagent again and by centrifugal detection method of reacting to reaction tube in the first step: while using this device to detect, first appropriate sample is placed in to pretreatment chamber (2), capping, jolting, centrifugal, carry out the 1st step reaction; Zai Cong pretreatment chamber (2) adds the 2nd step and above reaction reagent (6) again, capping, and jolting, centrifugal, carry out the 2nd step and above reaction, directly observe or testing result.
4, accompanying drawing 2 is applicable to the detection method of multistep reaction: while using this device to detect, the 1st step reaction reagent (6) is placed in reaction chamber (3) in advance, and appropriate sample is placed in to pretreatment chamber (2), capping, and jolting, centrifugal, carry out the 1st step reaction; The 2nd step and above reaction reagent (6) add from reaction chamber side opening (3.1), envelope side opening gland bonnet (3.2), and jolting, carries out the 2nd step and above reaction, directly observes or testing result.
Four, application example
1, the quick identification pipe of Nifedipine Tablets and detection method thereof
2, the quick identification pipe of gliclazid in tablets and detection method thereof
3, sibutramine rapid screening reaction tube and detection method thereof
Claims (6)
1. integrated chemical reaction device for fast detecting and a method thereof, is characterized in that:
Pick-up unit is a cylinder or cone shape airtight cavity; By gland bonnet (1), pretreatment chamber (2), reaction chamber (3), strainer tube (4), pretreating reagent (5) and reaction reagent (6), formed;
Its position and annexation are:
Pretreatment chamber (2) top arranges gland bonnet (1), and gland bonnet (1) is connected with pretreatment chamber (2) by screw thread or connection Jiao Qiao, plays sealing function;
Pretreatment chamber (2) is communicated with by strainer tube (4) up and down with reaction chamber (3); Micropore filter element or other filtering materials (4.1) are installed in strainer tube (4);
Pretreating reagent (5) is placed in pretreatment chamber (2) in advance, or puts in other container;
Reaction reagent (6) is placed in reaction chamber (3) in advance; Or add from side opening (3.1);
Detection method is for to be placed in pretreatment chamber (2) by appropriate sample, or adds pretreating reagent (5), capping, and jolting, centrifugal, reaction, observes or testing result.
2. by integrated chemical reaction device for fast detecting and method thereof claimed in claim 1, it is characterized in that:
Pretreatment chamber (2) external diameter is 2～50mm; Internal diameter is 1～48mm; Volume is 1～20ml; Sidewall has volume marking line.
3. by integrated chemical reaction device for fast detecting and method thereof claimed in claim 1, it is characterized in that:
Reaction chamber (3) external diameter is 2～50mm; Internal diameter is 1～48mm; Volume is 1～20ml; Sidewall has volume marking line; Bottom design becomes point end shape, is beneficial to collecting precipitation and observations.
4. by integrated chemical reaction device for fast detecting and method thereof claimed in claim 1, it is characterized in that:
Strainer tube (4) external diameter is 1～10mm; Internal diameter is 0.5～8mm; Volume is 0.2～10ml; The micropore filter element that it is built-in or other filtering materials (4.1) aperture are 0.22～20um; Upper and lower side is all designed to circular cone or dome shape, and center arranges aperture (4.2) and aperture (4.3), and aperture is 0.5～2mm.
5. by integrated chemical reaction device for fast detecting and method thereof claimed in claim 1, it is characterized in that:
Pretreating reagent (5) can be various organic solvents, acid-alkali salt, damping fluid and mixing thereof, and volume is 1～10ml; Be placed in advance pretreatment chamber (2); Its liquid level is lower than the upper top of strainer tube (4), and its liquid level, also lower than the upper top of strainer tube (4), guarantees that in storage and transportation, pretreating reagent (5) can not enter in reaction chamber (3) by strainer tube (4) when whole pick-up unit is inverted or is tilting.
6. by integrated chemical reaction device for fast detecting and method thereof claimed in claim 1, it is characterized in that:
Reaction reagent (6) is the reagent of the various chemical reactions of preparation, and volume is 0.5～10ml; Reaction reagent (6) divides in advance and installs in reaction chamber (3), its liquid level is lower than the lower bottom of strainer tube (4), and its liquid level, also lower than the lower bottom of strainer tube (4), guarantees that in storage and transportation, reaction reagent (6) can not enter in pretreatment chamber (2) by strainer tube (4) when whole pick-up unit is inverted or is tilting;
Reaction reagent (6) can freeze drying; Or make tablet or piller, be placed in reaction tube (2) bottom; Reach the object of extending the expiration date;
For there being the above detection method of 2 steps reactions, relate to the 2nd step and later reaction reagent (6) can add from side opening (3.1); Also can add in pretreatment chamber (2) and enter reaction chamber (3) by centrifugal method.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410185603.3A CN103969251A (en) | 2014-05-05 | 2014-05-05 | Device and method for quickly detecting integrated chemical reaction |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410185603.3A CN103969251A (en) | 2014-05-05 | 2014-05-05 | Device and method for quickly detecting integrated chemical reaction |
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| Publication Number | Publication Date |
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| CN103969251A true CN103969251A (en) | 2014-08-06 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410185603.3A Pending CN103969251A (en) | 2014-05-05 | 2014-05-05 | Device and method for quickly detecting integrated chemical reaction |
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| Country | Link |
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| CN (1) | CN103969251A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103868918A (en) * | 2014-04-01 | 2014-06-18 | 武汉百仕康生物科技有限公司 | Method for rapidly detecting illegal components added in Chinese patent medicines, healthcare foods, foods and cosmetics |
| CN104535710A (en) * | 2015-01-07 | 2015-04-22 | 中证检测科技（天津）有限公司 | Food detection device |
| CN105241874A (en) * | 2015-09-24 | 2016-01-13 | 广州安诺食品科学技术有限公司 | Rapid detection kit and detection method of hydroquinone in cosmetics |
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| CN2431276Y (en) * | 2000-06-08 | 2001-05-23 | 杨镇纵 | Purifying filter with porous filter membrane sterilizer for directly drinking running water |
| JP2003125753A (en) * | 2001-10-26 | 2003-05-07 | Hideo Tanaka | Plug for flask |
| WO2005124347A1 (en) * | 2004-06-15 | 2005-12-29 | Ani Biotech Oy | Filter device, the method, kit and use thereof |
| CN103364522A (en) * | 2013-07-18 | 2013-10-23 | 沈佳特 | Integrated rapid detection reaction tube |
| CN204255863U (en) * | 2014-05-05 | 2015-04-08 | 湖北省食品药品监督检验研究院 | A kind of integrated chemical reaction device for fast detecting |
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2014
- 2014-05-05 CN CN201410185603.3A patent/CN103969251A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2431276Y (en) * | 2000-06-08 | 2001-05-23 | 杨镇纵 | Purifying filter with porous filter membrane sterilizer for directly drinking running water |
| JP2003125753A (en) * | 2001-10-26 | 2003-05-07 | Hideo Tanaka | Plug for flask |
| WO2005124347A1 (en) * | 2004-06-15 | 2005-12-29 | Ani Biotech Oy | Filter device, the method, kit and use thereof |
| CN103364522A (en) * | 2013-07-18 | 2013-10-23 | 沈佳特 | Integrated rapid detection reaction tube |
| CN204255863U (en) * | 2014-05-05 | 2015-04-08 | 湖北省食品药品监督检验研究院 | A kind of integrated chemical reaction device for fast detecting |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103868918A (en) * | 2014-04-01 | 2014-06-18 | 武汉百仕康生物科技有限公司 | Method for rapidly detecting illegal components added in Chinese patent medicines, healthcare foods, foods and cosmetics |
| CN103868918B (en) * | 2014-04-01 | 2016-06-29 | 武汉纳达康生物科技有限公司 | The detection pipe of a kind of illegal adding ingredient and detection method |
| CN104535710A (en) * | 2015-01-07 | 2015-04-22 | 中证检测科技（天津）有限公司 | Food detection device |
| CN105241874A (en) * | 2015-09-24 | 2016-01-13 | 广州安诺食品科学技术有限公司 | Rapid detection kit and detection method of hydroquinone in cosmetics |
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Application publication date: 20140806 |