CN105241874A - Rapid detection kit and detection method of hydroquinone in cosmetics - Google Patents
Rapid detection kit and detection method of hydroquinone in cosmetics Download PDFInfo
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- CN105241874A CN105241874A CN201510615993.8A CN201510615993A CN105241874A CN 105241874 A CN105241874 A CN 105241874A CN 201510615993 A CN201510615993 A CN 201510615993A CN 105241874 A CN105241874 A CN 105241874A
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- quinhydrones
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- 239000002537 cosmetic Substances 0.000 title claims abstract description 40
- 238000001514 detection method Methods 0.000 title claims abstract description 38
- QIGBRXMKCJKVMJ-UHFFFAOYSA-N Hydroquinone Chemical compound OC1=CC=C(O)C=C1 QIGBRXMKCJKVMJ-UHFFFAOYSA-N 0.000 title abstract description 10
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims abstract description 42
- 238000006243 chemical reaction Methods 0.000 claims abstract description 32
- 239000000243 solution Substances 0.000 claims abstract description 32
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 26
- 239000000203 mixture Substances 0.000 claims abstract description 19
- 239000003513 alkali Substances 0.000 claims abstract description 16
- YMWUJEATGCHHMB-UHFFFAOYSA-N Dichloromethane Chemical compound ClCCl YMWUJEATGCHHMB-UHFFFAOYSA-N 0.000 claims abstract description 15
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 claims abstract description 15
- 239000002904 solvent Substances 0.000 claims abstract description 15
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 15
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 12
- KWYUFKZDYYNOTN-UHFFFAOYSA-M Potassium hydroxide Chemical compound [OH-].[K+] KWYUFKZDYYNOTN-UHFFFAOYSA-M 0.000 claims abstract description 12
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 claims abstract description 10
- 239000008213 purified water Substances 0.000 claims abstract description 10
- 239000007787 solid Substances 0.000 claims abstract description 9
- KEAYESYHFKHZAL-UHFFFAOYSA-N Sodium Chemical compound [Na] KEAYESYHFKHZAL-UHFFFAOYSA-N 0.000 claims abstract description 4
- 229910000104 sodium hydride Inorganic materials 0.000 claims abstract description 4
- 239000012312 sodium hydride Substances 0.000 claims abstract description 4
- 239000007864 aqueous solution Substances 0.000 claims abstract description 3
- 238000002474 experimental method Methods 0.000 claims abstract description 3
- 239000003960 organic solvent Substances 0.000 claims abstract description 3
- 238000012360 testing method Methods 0.000 claims description 24
- 239000002585 base Substances 0.000 claims description 9
- 238000010790 dilution Methods 0.000 claims description 5
- 239000012895 dilution Substances 0.000 claims description 5
- 238000001819 mass spectrum Methods 0.000 claims description 4
- 238000004811 liquid chromatography Methods 0.000 claims description 3
- 239000012153 distilled water Substances 0.000 claims description 2
- 239000000463 material Substances 0.000 claims description 2
- 230000007935 neutral effect Effects 0.000 claims 1
- 208000003351 Melanosis Diseases 0.000 abstract description 10
- 230000002087 whitening effect Effects 0.000 abstract description 10
- 230000008859 change Effects 0.000 abstract description 2
- 206010014970 Ephelides Diseases 0.000 abstract 1
- 235000019441 ethanol Nutrition 0.000 abstract 1
- 238000000034 method Methods 0.000 description 11
- 239000000126 substance Substances 0.000 description 6
- 239000006071 cream Substances 0.000 description 5
- 230000008569 process Effects 0.000 description 5
- AZQWKYJCGOJGHM-UHFFFAOYSA-N 1,4-benzoquinone Chemical compound O=C1C=CC(=O)C=C1 AZQWKYJCGOJGHM-UHFFFAOYSA-N 0.000 description 4
- 230000003020 moisturizing effect Effects 0.000 description 4
- 229940005561 1,4-benzoquinone Drugs 0.000 description 3
- UHOVQNZJYSORNB-UHFFFAOYSA-N Benzene Chemical compound C1=CC=CC=C1 UHOVQNZJYSORNB-UHFFFAOYSA-N 0.000 description 3
- 230000008901 benefit Effects 0.000 description 3
- 238000003822 preparative gas chromatography Methods 0.000 description 3
- 238000004458 analytical method Methods 0.000 description 2
- 238000004128 high performance liquid chromatography Methods 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 239000011159 matrix material Substances 0.000 description 2
- 238000005259 measurement Methods 0.000 description 2
- BDJXVNRFAQSMAA-UHFFFAOYSA-N quinhydrone Chemical compound OC1=CC=C(O)C=C1.O=C1C=CC(=O)C=C1 BDJXVNRFAQSMAA-UHFFFAOYSA-N 0.000 description 2
- 229940052881 quinhydrone Drugs 0.000 description 2
- 230000004044 response Effects 0.000 description 2
- 150000003839 salts Chemical class 0.000 description 2
- 238000012216 screening Methods 0.000 description 2
- 230000035945 sensitivity Effects 0.000 description 2
- 206010019233 Headaches Diseases 0.000 description 1
- UFHFLCQGNIYNRP-UHFFFAOYSA-N Hydrogen Chemical compound [H][H] UFHFLCQGNIYNRP-UHFFFAOYSA-N 0.000 description 1
- 206010067125 Liver injury Diseases 0.000 description 1
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 1
- 241000238370 Sepia Species 0.000 description 1
- 208000009205 Tinnitus Diseases 0.000 description 1
- 230000003796 beauty Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- UREBDLICKHMUKA-DVTGEIKXSA-N betamethasone Chemical compound C1CC2=CC(=O)C=C[C@]2(C)[C@]2(F)[C@@H]1[C@@H]1C[C@H](C)[C@@](C(=O)CO)(O)[C@@]1(C)C[C@@H]2O UREBDLICKHMUKA-DVTGEIKXSA-N 0.000 description 1
- 229960002537 betamethasone Drugs 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 238000005282 brightening Methods 0.000 description 1
- 210000003169 central nervous system Anatomy 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 230000008878 coupling Effects 0.000 description 1
- 238000010168 coupling process Methods 0.000 description 1
- 238000005859 coupling reaction Methods 0.000 description 1
- 239000013078 crystal Substances 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 239000006185 dispersion Substances 0.000 description 1
- 208000002173 dizziness Diseases 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000006870 function Effects 0.000 description 1
- 231100000869 headache Toxicity 0.000 description 1
- 229910052739 hydrogen Inorganic materials 0.000 description 1
- 239000001257 hydrogen Substances 0.000 description 1
- 125000002887 hydroxy group Chemical group [H]O* 0.000 description 1
- 238000004895 liquid chromatography mass spectrometry Methods 0.000 description 1
- 210000004400 mucous membrane Anatomy 0.000 description 1
- 230000006855 networking Effects 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 239000000049 pigment Substances 0.000 description 1
- 231100000614 poison Toxicity 0.000 description 1
- 230000007096 poisonous effect Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 210000003491 skin Anatomy 0.000 description 1
- 230000004215 skin function Effects 0.000 description 1
- 238000001228 spectrum Methods 0.000 description 1
- 208000024891 symptom Diseases 0.000 description 1
- 231100000886 tinnitus Toxicity 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
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- Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
Abstract
The invention provides a rapid detection kit and a detection method of hydroquinone in cosmetics. The rapid detection kit comprises a solvent reagent and an alkaline reagent, and experiment expendables such as reaction bottles, pipettes, and sample spoons; wherein the solvent reagent is purified water, or one or a mixture of a plurality of organic solvents selected from ethyl alcohol, methyl alcohol, ether, and dichloromethane; the alkaline reagent is an alkaline ionic solution or a solid alkali; the alkaline ionic solution is one selected from sodium hydroxide, potassium hydroxide, sodium hydride, or aqueous solutions of sodium hydroxide, potassium hydroxide, and sodium hydride; and preferably the concentration of the alkaline ionic solution ranges from 0.5 to 5%. The rapid detection method is mainly used for rapidly detecting that whether hydroquinone is illegally added into cosmetics which are used for whitening and removing freckles; operation of the rapid detection method is simple and convenient; no precise or large-scale equipment is needed; detection time is short; detection can be finished in 2 to 5min; judgment can be made only via direct observation of color change; and detection steps are simplified greatly.
Description
Technical field
The present invention relates to quick detection kit and the detection method thereof of quinhydrones in a kind of cosmetics.
Background technology
The current raising along with domestic level, the demand of women to beauty treatment is increasing, various whitening product also emerges in an endless stream, add in cosmetics various whitening function composition also right and wrong usually see, quinhydrones is one of them, although treatment pigment increase chemical composition a lot, but quinhydrones is cheap, be easy to get, all there is the feature of sale in each chemical reagent shop, makes illegal situation of adding in cosmetics very common.In sampling observation to networking cosmetic of China 2014, quinhydrones, phenol or betamethasone chemical composition in most of whitening, Freckle removing cosmetics, are all detected.
Quinhydrones has another name called p-dihydroxy-benzene, it is the compound that two contraposition hydrogen of benzene are optionally substituted by a hydroxyl group formation, white crystals, p-dihydroxy-benzene is poisonous, and adult wrongly takes 1 gram, the symptoms such as headache, dizziness, tinnitus, pale complexion can be there is, further, because quinhydrones toxicity is very large, strong corrosive attack is had to skin, mucous membrane, central nervous system can be suppressed or damage liver, skin function, so it is classified as the banned substance in whitening, Freckle removing cosmetics by our country all the time.
At present for the detection adding quinhydrones illegal in cosmetics, mainly adopt the method for high performance liquid chromatography, liquid chromatography mass coupling.High performance liquid chromatography and LC-MS chromatographic process are state-of-the-art modern analysis means, it has, and separation efficiency is high, sensitivity advantages of higher, adopt standard control method, point-devicely can detect the content of quinhydrones in sample, but these need the checkout equipment of specialty, operating process is more complicated all, experimental period is long, experimental cost is high, are very large tests for the patience of Site Detection personnel, be therefore necessary to research and develop a kind of kit that can detect fast the carrying out of quinhydrones in cosmetics and detection method thereof.
Summary of the invention
The object of the invention is to for the deficiencies in the prior art, provide one very simple, fast, analysis cost is very low, strong, highly sensitive and be applicable to the kit of field quick detection to the specificity of quinhydrones.
The technical solution used in the present invention is as follows:
A quick detection kit for quinhydrones in cosmetics utilizes following several character of quinhydrones to realize detecting fast it.
1, quinhydrones and alkali ion form quinhydrones salt, and quinhydrones salt character is very unstable, are very fastly oxidized to 1,4-benzoquinone, and 1,4-benzoquinone and quinhydrones generate the molecular complex quinhydrone of quinhydrones-1,4-benzoquinone.
2, this reaction is very sensitive, even if also can very fast response in the matrix of cosmetics, cosmetic base affects its reaction hardly, matrix in addition in cosmetics does not almost have and alkali ion generation metachromasia, therefore, quinhydrones need not extract and can complete by this reaction from cosmetics, and testing process is simple and quick.
3, quinhydrone is blackish green, and color is very dark, and be very easy to show in the background color of cosmetics, therefore, the detectability reached is very low, can reach detect a small amount of object of adding fast completely.
Utilize the above-mentioned character of quinhydrones, quick detection kit provided by the invention comprises following component:
Described kit comprises solvent reagent and alkaline reagent, be equipped with the experiment consumptive materials such as reaction bulb, suction pipe, sample spoon, be assembled into kit, the reagent proportioning of taking in a certain amount of water and kit according to testing sample situation is only needed to observe colour developing during use, can judge whether add quinhydrones composition in testing sample, wherein said solvent reagent is one or more of the organic solvent such as purified water or ethanol, methyl alcohol, ether, methylene chloride.
Described alkaline reagent is basic ion solution or solid base, and described basic ion solution is the one of NaOH, potassium hydroxide, sodium hydride and aqueous solution thereof.
The concentration of described alkali ion solution is 0.5 ~ 5%, is wherein preferably 1.5 ~ 3.0%, because the alkali ion in the too high solution of concentration is easily by the chemical composition oxidation in cosmetics, disturbance reponse served by band, affects result and judges.
Another object of the present invention is to provide a kind of method for quick utilizing mentioned reagent box to detect quinhydrones chemical composition in cosmetics, and concrete steps are as follows:
1, get testing sample in tool reaction bulb, in testing sample, add appropriate solvent reagent dilutions, ensure that cosmetics have certain mobility;
2, drip appropriate alkaline solion or solid base in test sample to treating after dilution, shake up immediately, leave standstill under normal temperature and observe colour developing situation after 2-5 minute;
3, colour developing situation is observed: when reaction bulb occurs brown color to brownish black, then tentatively can judge that these cosmetics may with the addition of quinhydrones composition, if chromogenic reaction is unchanged, then illustrate in these cosmetics and do not add quinhydrones composition, and then the sample that may be added with quinhydrones carries out the meticulousr detection such as mass spectrum, liquid chromatography again.
In above-mentioned steps 1, described solvent reagent can be the one in purified water or ethanol, methyl alcohol, ether, methylene chloride, is wherein preferably purified water or distilled water.
In above-mentioned steps 1, the consumption of described solvent reagent is 0 ~ 10ml, and testing sample amount is 0.1 ~ 10g or 1 ~ 10ml, and the volume of described solvent reagent can 0.5 ~ 2 times of preferred institute's sample thief cosmetics amount, cosmetics dilution can be kept certain mobility like this, can Detection results be ensured again.
In above-mentioned steps 2, the alkali ion solution added or solid base are preferably alkali ion solution, and alkali ion Solution Dispersion is good, can be dispersed in very short time after adding, intensified response effect.
In above-mentioned steps 2, the addition of described solid base is 1mg ~ 1g.
The alkali ion solution added in above-mentioned steps 2 is preferably concentration 2.0 ~ 3.0% sodium hydroxide solution.
The volume adding alkali ion solution in above-mentioned steps 2 is preferably 50 ~ 1000 microlitres.
For the testing sample of some inherently liquid type, can directly add alkali ion solution, save the process of step 1.
Compared with prior art, the present invention has following beneficial effect:
Whether method for quick of the present invention mainly illegally adds the rapid screening carrying out quinhydrones chemical composition in brightening, dispelling spots, dispelling class cosmetics, have sensitivity very high, colour developing is obvious, the advantage that testing result accuracy rate is higher, accuracy rate can reach more than 99%, and false negative rate is zero, in addition, detection method of the present invention is simple to operate, convenient, do not need precision or large-scale instrument, detection time is short, detection can be completed in general 2 ~ 5 minutes, and testing result is intuitively by seeing that color change just can judge, be applicable to very much from gross sample, pick out a small amount of suspicious specimen, enormously simplify the step of detection, and only need sample suspicious on a small quantity to carry out mass spectrum again, the Precision measurement such as chromatogram, this also makes testing cost greatly reduce, be applicable to very much the needs of supervision department's rapid screening, to the operational paradigm improving supervision department, rapid strike lawless person has great social benefit.
Embodiment
Below in conjunction with specific embodiments of the invention, technical scheme in the embodiment of the present invention is clearly and completely described, obviously, described embodiment is only the present invention's part embodiment, instead of whole embodiments, when not conflicting, the embodiment in the application and the feature in embodiment can combine mutually, describe the present invention in detail below according to each embodiment.
Embodiment 1:
Getting the clean white flawless essence cream 1g of certain brand adds in reaction bulb, add pure water 1ml, shake up to be measured, the sodium hydroxide solution that 250 lli are 0.5% is dripped in reaction bulb, shake up, observe after 2 minutes, reaction bulb content shows sepia, then can judge in the clean white flawless essence cream of the present embodiment containing quinhydrones composition.
Embodiment 2:
Getting certain brand skin-whitening speckle-removing cream 1ml adds in reaction bulb, add pure water 1ml, shake up to be measured, the sodium hydroxide solution that 150 lli are 2% is dripped in reaction bulb, shake up, observe after 5 minutes, reaction bulb content shows brownish black, then can judge in the present embodiment skin-whitening speckle-removing cream containing quinhydrones composition.
Embodiment 3:
Getting the tender bloom 1ml of certain brand nti-freckle adds in reaction bulb, add ethanol 1ml, shake up to be measured, the sodium hydroxide solution that 250 lli are 2.5% is dripped in reaction bulb, shake up, observe after 2 ~ 3 minutes, reaction bulb content shows brown color, then can judge in the tender bloom of the present embodiment nti-freckle containing quinhydrones composition.
Implement 4:
Getting certain brand freckle removing and whitening element frost 1ml adds in reaction bulb, add purified water 1ml, shake up to be measured, the sodium hydroxide solution that 250 lli are 1.5% is dripped in reaction bulb, shake up, observe after 5 minutes, reaction bulb content does not have variable color, then can judge in this freckle removing and whitening element frost not containing quinhydrones composition.
Implement 5:
Get certain brand whitening and moisturizing moisturizing freckle-removing mask a slice, extruding liquid 1ml adds in reaction bulb, directly to dripping the sodium hydroxide solution that 250 lli are 3% in reaction bulb, shake up, observe after 3 minutes, reaction bulb content shows brownish black, then can judge in the present embodiment whitening and moisturizing moisturizing freckle-removing mask containing quinhydrones composition.
Implement 6:
Getting certain brand whitening day cream 10g adds in reaction bulb, adds purified water 1ml, shakes up to be measured, in reaction bulb, drip 1500 lli is 5% sodium hydroxide solution, shakes up, and observes after 5 minutes, reaction bulb content shows brownish black, then can judge in the tender bloom of the present embodiment nti-freckle containing quinhydrones composition.
Example 7 quinhydrones detects situation contrast
Whether adopt described in example 6 and detect reagent and detection method, carry out contrast testing result, detect 30 batch samples altogether with vapor-phase chromatography, first detect containing quinhydrones composition in judgement sample by this law, then compare by vapor-phase chromatography, result is as follows:
| Sample number into spectrum | This method | Vapor-phase chromatography |
| 001 | - | - |
| 002 | - | - |
| 003 | + | + |
| 004 | - | - |
| 005 | - | - |
| 006 | - | - |
| 007 | - | - |
| 008 | - | - |
| 009 | + | + |
| 010 | + | + |
| 011 | - | - |
| 012 | - | - |
| 013 | - | - |
| 014 | - | - |
| 015 | - | - |
| 016 | - | - |
| 017 | - | - |
| 018 | - | - |
| 019 | + | + |
| 020 | - | - |
| 021 | - | - |
| 022 | + | + |
| 023 | - | - |
| 024 | - | - |
| 025 | - | - |
| 026 | - | - |
| 027 | - | - |
| 028 | - | - |
| 029 | + | + |
| 030 | - | - |
Note: "-" expression does not detect, "+" expression detects the positive.
As can be seen from the above table, the identical rate of two kinds of methods is 100%, visible chromogenic reaction result accuracy rate of the present invention is higher, false positive rate is 0, suspicious specimen can narrow down to very among a small circle by the present invention rapidly, finally only need to carry out the Precision measurement such as chromatogram, mass spectrum to these few suspicious specimen, the illegal sample containing quinhydrones can be detected.
The foregoing is only embodiments of the invention; not thereby the scope of the claims of the present invention is limited; every utilize description of the present invention to do equivalent structure or equivalent flow process conversion; or be directly or indirectly used in other relevant technical fields, be all in like manner included in scope of patent protection of the present invention.
Claims (10)
1. the quick detection kit of quinhydrones in cosmetics, is characterized in that: described kit comprises solvent reagent and alkaline reagent, is equipped with the experiment consumptive materials such as reaction bulb, suction pipe, sample spoon;
Wherein, described solvent reagent is one or more of the organic solvent such as purified water or ethanol, methyl alcohol, ether, methylene chloride;
Described alkaline reagent is basic ion solution or solid base, and described basic ion solution is the one of NaOH, potassium hydroxide, sodium hydride and aqueous solution thereof, and be wherein preferably basic ion solution, the concentration of described alkali ion solution is preferably 0.5 ~ 5%.
2. the quick detection kit of quinhydrones in cosmetics according to claim 1, is characterized in that: it is 1.5 ~ 3.0% sodium hydroxide solutions that described basic ion solution is preferably concentration.
3. the quick detection kit of quinhydrones in cosmetics according to claim 1, it is characterized in that: described solvent reagent is purified water, described alkaline reagent is concentration is 2.0 ~ 2.5% sodium hydroxide solutions.
4. the quick detection method for testing of quinhydrones in cosmetics as claimed in claim 1 or 2, is characterized in that: comprise the following steps:
(1), get testing sample in reaction bulb, in testing sample, add appropriate solvent reagent dilutions, ensure that cosmetics have certain mobility;
(2), to treating after dilution drip appropriate alkaline solion or solid base in test sample, shake up immediately, leave standstill under normal temperature and observe colour developing situation after 2-5 minute;
(3), colour developing situation is observed: when reaction bulb occurs brown color to brownish black, then tentatively can judge that these cosmetics may with the addition of quinhydrones composition, if chromogenic reaction is unchanged, then illustrate in these cosmetics and do not add quinhydrones composition, and then the sample that may be added with quinhydrones carries out the meticulousr detection such as mass spectrum, liquid chromatography again.
5. the quick detection method for testing of quinhydrones in cosmetics according to claim 4, it is characterized in that: the solvent reagent in described step (1) can be the one in purified water or ethanol, methyl alcohol, ether, methylene chloride, is wherein preferably purified water or distilled water.
6. the quick detection method for testing of quinhydrones in cosmetics according to claim 4, it is characterized in that: the consumption of the solvent reagent in described step (1) is 0 ~ 10ml, testing sample amount is 0.1 ~ 10g or 1 ~ 10ml, and the volume of described solvent reagent can 0.5 ~ 2 times of preferred institute's sample thief cosmetics amount.
7. the quick detection method for testing of quinhydrones in cosmetics according to claim 4, is characterized in that: the alkali ion solution added in described step (2) or solid base, is wherein preferably alkali ion solution.
8. the quick detection method for testing of quinhydrones in cosmetics according to claim 4, is characterized in that: in described step (2), the addition of solid base is 1mg ~ 1g.
9. the quick detection method for testing of quinhydrones in cosmetics according to claim 4, is characterized in that: described step (2) neutral and alkali solion is preferably concentration 2.0 ~ 3.0% sodium hydroxide solution.
10. the quick detection method for testing of quinhydrones in cosmetics according to claim 4, is characterized in that: the volume adding alkali ion solution in described step (2) is preferably 50 ~ 1000 microlitres.
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| CN201510615993.8A CN105241874A (en) | 2015-09-24 | 2015-09-24 | Rapid detection kit and detection method of hydroquinone in cosmetics |
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| CN201510615993.8A CN105241874A (en) | 2015-09-24 | 2015-09-24 | Rapid detection kit and detection method of hydroquinone in cosmetics |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106932525A (en) * | 2017-03-02 | 2017-07-07 | 广州安诺食品科学技术有限公司 | Auramine O quick detection kit, detection method and the application of medicinal material in the detection |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1563955A (en) * | 2004-03-17 | 2005-01-12 | 周强 | Reagent for detecting harmful ingredient in cosmetics |
| CN1766586A (en) * | 2005-09-29 | 2006-05-03 | 浙江省疾病预防控制中心 | A rapid detection reagent for hydroquinone and its detection method and application |
| US20080076670A1 (en) * | 2004-03-24 | 2008-03-27 | Uri Sivan | Artificial receptors |
| CN101581694A (en) * | 2009-06-20 | 2009-11-18 | 西北师范大学 | Electrochemical detection method for quinhydrone |
| CN102109464A (en) * | 2009-12-25 | 2011-06-29 | 中国科学院沈阳应用生态研究所 | Method of detecting content of hydroquinone in soil |
| CN103969251A (en) * | 2014-05-05 | 2014-08-06 | 湖北省食品药品监督检验研究院 | Device and method for quickly detecting integrated chemical reaction |
| CN104569187A (en) * | 2014-12-17 | 2015-04-29 | 深圳出入境检验检疫局食品检验检疫技术中心 | Detection method and device for whitening and freckle-removing components in cosmetics |
-
2015
- 2015-09-24 CN CN201510615993.8A patent/CN105241874A/en active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1563955A (en) * | 2004-03-17 | 2005-01-12 | 周强 | Reagent for detecting harmful ingredient in cosmetics |
| US20080076670A1 (en) * | 2004-03-24 | 2008-03-27 | Uri Sivan | Artificial receptors |
| CN1766586A (en) * | 2005-09-29 | 2006-05-03 | 浙江省疾病预防控制中心 | A rapid detection reagent for hydroquinone and its detection method and application |
| CN101581694A (en) * | 2009-06-20 | 2009-11-18 | 西北师范大学 | Electrochemical detection method for quinhydrone |
| CN102109464A (en) * | 2009-12-25 | 2011-06-29 | 中国科学院沈阳应用生态研究所 | Method of detecting content of hydroquinone in soil |
| CN103969251A (en) * | 2014-05-05 | 2014-08-06 | 湖北省食品药品监督检验研究院 | Device and method for quickly detecting integrated chemical reaction |
| CN104569187A (en) * | 2014-12-17 | 2015-04-29 | 深圳出入境检验检疫局食品检验检疫技术中心 | Detection method and device for whitening and freckle-removing components in cosmetics |
Non-Patent Citations (3)
| Title |
|---|
| 浮田忠之进等: "《汞的分析方法》", 31 March 1979, 原子能出版社 * |
| 王箴: "《化工辞典》", 31 December 1979, 化学工业出版社 * |
| 郑星泉，周淑玉等: "《化妆品卫生检验手册》", 31 August 2003, 化学工业出版社 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106932525A (en) * | 2017-03-02 | 2017-07-07 | 广州安诺食品科学技术有限公司 | Auramine O quick detection kit, detection method and the application of medicinal material in the detection |
| CN106932525B (en) * | 2017-03-02 | 2018-12-21 | 广州安诺科技股份有限公司 | Auramine O quick detection kit, detection method and the application of medicinal material in the detection |
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Address after: 511000 Guangzhou High-tech Industrial Development Zone, Guangzhou, Guangdong Province, No. 3 Lanyue Road, Science City, No. G204, G District, Guangzhou International Business Incubator Applicant after: Guangzhou Annuo Polytron Technologies Inc Address before: 511000 Guangzhou High-tech Industrial Development Zone, Guangzhou, Guangdong Province, No. 3 Lanyue Road, Science City, No. G204, G District, Guangzhou International Business Incubator Applicant before: GUANGZHOU ANNUO FOOD SCIENCE AND TECHNOLOGY CO., LTD. |
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Application publication date: 20160113 |