CN103966149A - 一种生活垃圾和污泥腐熟菌剂及其应用和所制腐熟基质 - Google Patents
一种生活垃圾和污泥腐熟菌剂及其应用和所制腐熟基质 Download PDFInfo
- Publication number
- CN103966149A CN103966149A CN201410239327.4A CN201410239327A CN103966149A CN 103966149 A CN103966149 A CN 103966149A CN 201410239327 A CN201410239327 A CN 201410239327A CN 103966149 A CN103966149 A CN 103966149A
- Authority
- CN
- China
- Prior art keywords
- heap
- fermentation
- life
- mud
- temperature
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 239000010813 municipal solid waste Substances 0.000 title claims abstract description 62
- 239000003795 chemical substances by application Substances 0.000 title claims abstract description 60
- 241000894006 Bacteria Species 0.000 title claims abstract description 42
- 239000011159 matrix material Substances 0.000 title claims abstract description 39
- 239000010802 sludge Substances 0.000 title claims abstract description 11
- 238000000855 fermentation Methods 0.000 claims abstract description 54
- 230000004151 fermentation Effects 0.000 claims abstract description 54
- 238000000034 method Methods 0.000 claims abstract description 54
- 241000881860 Paenibacillus mucilaginosus Species 0.000 claims abstract description 33
- 239000007633 bacillus mucilaginosus Substances 0.000 claims abstract description 33
- 230000008569 process Effects 0.000 claims abstract description 33
- 241000187395 Streptomyces microflavus Species 0.000 claims abstract description 29
- 230000001877 deodorizing effect Effects 0.000 claims abstract description 16
- 240000008067 Cucumis sativus Species 0.000 claims abstract description 15
- 235000010799 Cucumis sativus var sativus Nutrition 0.000 claims abstract description 15
- 239000010902 straw Substances 0.000 claims abstract description 13
- 240000008042 Zea mays Species 0.000 claims abstract description 12
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims abstract description 12
- UHPMCKVQTMMPCG-UHFFFAOYSA-N 5,8-dihydroxy-2-methoxy-6-methyl-7-(2-oxopropyl)naphthalene-1,4-dione Chemical compound CC1=C(CC(C)=O)C(O)=C2C(=O)C(OC)=CC(=O)C2=C1O UHPMCKVQTMMPCG-UHFFFAOYSA-N 0.000 claims abstract description 9
- 241000223218 Fusarium Species 0.000 claims abstract description 9
- 241000228212 Aspergillus Species 0.000 claims abstract description 4
- 244000063299 Bacillus subtilis Species 0.000 claims abstract description 4
- 235000014469 Bacillus subtilis Nutrition 0.000 claims abstract description 4
- 241000233866 Fungi Species 0.000 claims description 48
- 239000002131 composite material Substances 0.000 claims description 47
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 39
- 230000001580 bacterial effect Effects 0.000 claims description 34
- 230000007306 turnover Effects 0.000 claims description 30
- 239000002068 microbial inoculum Substances 0.000 claims description 29
- 241000228245 Aspergillus niger Species 0.000 claims description 25
- 239000002023 wood Substances 0.000 claims description 25
- 229920002472 Starch Polymers 0.000 claims description 21
- 239000008107 starch Substances 0.000 claims description 21
- 235000019698 starch Nutrition 0.000 claims description 21
- 238000004519 manufacturing process Methods 0.000 claims description 19
- 239000002994 raw material Substances 0.000 claims description 12
- 235000016383 Zea mays subsp huehuetenangensis Nutrition 0.000 claims description 11
- 235000009973 maize Nutrition 0.000 claims description 11
- 239000000463 material Substances 0.000 claims description 10
- 201000010099 disease Diseases 0.000 claims description 9
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims description 9
- 210000003608 fece Anatomy 0.000 claims description 8
- 239000010871 livestock manure Substances 0.000 claims description 8
- 235000015097 nutrients Nutrition 0.000 claims description 8
- 239000001913 cellulose Substances 0.000 claims description 7
- 229920002678 cellulose Polymers 0.000 claims description 7
- 150000001875 compounds Chemical class 0.000 claims description 7
- 229910052700 potassium Inorganic materials 0.000 claims description 7
- 102000004169 proteins and genes Human genes 0.000 claims description 7
- 108090000623 proteins and genes Proteins 0.000 claims description 7
- 230000000630 rising effect Effects 0.000 claims description 7
- 230000006870 function Effects 0.000 claims description 6
- 235000006008 Brassica napus var napus Nutrition 0.000 claims description 5
- 235000001169 Brassica oleracea var oleracea Nutrition 0.000 claims description 5
- 241001674939 Caulanthus Species 0.000 claims description 5
- 238000000354 decomposition reaction Methods 0.000 claims description 5
- 230000003203 everyday effect Effects 0.000 claims description 5
- 239000005416 organic matter Substances 0.000 claims description 4
- 229910052698 phosphorus Inorganic materials 0.000 claims description 4
- 241000223259 Trichoderma Species 0.000 claims description 3
- CKUAXEQHGKSLHN-UHFFFAOYSA-N [C].[N] Chemical compound [C].[N] CKUAXEQHGKSLHN-UHFFFAOYSA-N 0.000 claims description 3
- 230000015572 biosynthetic process Effects 0.000 claims description 3
- 238000013467 fragmentation Methods 0.000 claims description 3
- 238000006062 fragmentation reaction Methods 0.000 claims description 3
- 229910052757 nitrogen Inorganic materials 0.000 claims description 3
- 238000002203 pretreatment Methods 0.000 claims description 3
- 239000000203 mixture Substances 0.000 abstract description 6
- 238000002156 mixing Methods 0.000 abstract description 4
- 241000223262 Trichoderma longibrachiatum Species 0.000 abstract 1
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 abstract 1
- 235000005822 corn Nutrition 0.000 abstract 1
- 239000000575 pesticide Substances 0.000 abstract 1
- 239000000243 solution Substances 0.000 description 33
- 239000007788 liquid Substances 0.000 description 28
- 230000000694 effects Effects 0.000 description 25
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 20
- 238000012360 testing method Methods 0.000 description 17
- 239000012153 distilled water Substances 0.000 description 16
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 15
- 239000008103 glucose Substances 0.000 description 15
- 239000002609 medium Substances 0.000 description 14
- 102000004190 Enzymes Human genes 0.000 description 13
- 108090000790 Enzymes Proteins 0.000 description 13
- 229940088598 enzyme Drugs 0.000 description 13
- 229910000037 hydrogen sulfide Inorganic materials 0.000 description 12
- RWSOTUBLDIXVET-UHFFFAOYSA-N Dihydrogen sulfide Chemical compound S RWSOTUBLDIXVET-UHFFFAOYSA-N 0.000 description 11
- 229910021529 ammonia Inorganic materials 0.000 description 11
- 238000012545 processing Methods 0.000 description 10
- 239000007787 solid Substances 0.000 description 10
- 229920001817 Agar Polymers 0.000 description 9
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 9
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 description 9
- 239000008272 agar Substances 0.000 description 9
- 239000003337 fertilizer Substances 0.000 description 9
- 238000002360 preparation method Methods 0.000 description 9
- 230000001954 sterilising effect Effects 0.000 description 9
- 238000004448 titration Methods 0.000 description 9
- 244000068988 Glycine max Species 0.000 description 8
- 235000010469 Glycine max Nutrition 0.000 description 8
- 239000000126 substance Substances 0.000 description 8
- 238000006243 chemical reaction Methods 0.000 description 7
- 238000002474 experimental method Methods 0.000 description 7
- ZCYVEMRRCGMTRW-UHFFFAOYSA-N 7553-56-2 Chemical compound [I] ZCYVEMRRCGMTRW-UHFFFAOYSA-N 0.000 description 6
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 6
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 6
- 244000061456 Solanum tuberosum Species 0.000 description 6
- 235000002595 Solanum tuberosum Nutrition 0.000 description 6
- 229910000831 Steel Inorganic materials 0.000 description 6
- 239000002361 compost Substances 0.000 description 6
- 238000001514 detection method Methods 0.000 description 6
- 229910052740 iodine Inorganic materials 0.000 description 6
- 239000011630 iodine Substances 0.000 description 6
- 230000000813 microbial effect Effects 0.000 description 6
- 238000004321 preservation Methods 0.000 description 6
- 239000010959 steel Substances 0.000 description 6
- 239000001117 sulphuric acid Substances 0.000 description 6
- 235000011149 sulphuric acid Nutrition 0.000 description 6
- 108010059892 Cellulase Proteins 0.000 description 5
- 230000004913 activation Effects 0.000 description 5
- 230000008485 antagonism Effects 0.000 description 5
- KGBXLFKZBHKPEV-UHFFFAOYSA-N boric acid Chemical compound OB(O)O KGBXLFKZBHKPEV-UHFFFAOYSA-N 0.000 description 5
- 239000004327 boric acid Substances 0.000 description 5
- 229940106157 cellulase Drugs 0.000 description 5
- 239000003153 chemical reaction reagent Substances 0.000 description 5
- 239000003814 drug Substances 0.000 description 5
- 239000001963 growth medium Substances 0.000 description 5
- 235000018102 proteins Nutrition 0.000 description 5
- 102000013142 Amylases Human genes 0.000 description 4
- 108010065511 Amylases Proteins 0.000 description 4
- 108091005804 Peptidases Proteins 0.000 description 4
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 description 4
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 4
- 230000008859 change Effects 0.000 description 4
- 238000001816 cooling Methods 0.000 description 4
- 239000000706 filtrate Substances 0.000 description 4
- 235000013336 milk Nutrition 0.000 description 4
- 239000008267 milk Substances 0.000 description 4
- 210000004080 milk Anatomy 0.000 description 4
- 238000010899 nucleation Methods 0.000 description 4
- 239000008188 pellet Substances 0.000 description 4
- 239000011591 potassium Substances 0.000 description 4
- 230000035484 reaction time Effects 0.000 description 4
- 238000012807 shake-flask culturing Methods 0.000 description 4
- 239000002689 soil Substances 0.000 description 4
- 239000000758 substrate Substances 0.000 description 4
- -1 15 grams Substances 0.000 description 3
- LWFUFLREGJMOIZ-UHFFFAOYSA-N 3,5-dinitrosalicylic acid Chemical compound OC(=O)C1=CC([N+]([O-])=O)=CC([N+]([O-])=O)=C1O LWFUFLREGJMOIZ-UHFFFAOYSA-N 0.000 description 3
- 239000004382 Amylase Substances 0.000 description 3
- 239000004365 Protease Substances 0.000 description 3
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 description 3
- ZOIORXHNWRGPMV-UHFFFAOYSA-N acetic acid;zinc Chemical compound [Zn].CC(O)=O.CC(O)=O ZOIORXHNWRGPMV-UHFFFAOYSA-N 0.000 description 3
- 235000019418 amylase Nutrition 0.000 description 3
- 230000003042 antagnostic effect Effects 0.000 description 3
- 230000003385 bacteriostatic effect Effects 0.000 description 3
- FFBHFFJDDLITSX-UHFFFAOYSA-N benzyl N-[2-hydroxy-4-(3-oxomorpholin-4-yl)phenyl]carbamate Chemical compound OC1=C(NC(=O)OCC2=CC=CC=C2)C=CC(=C1)N1CCOCC1=O FFBHFFJDDLITSX-UHFFFAOYSA-N 0.000 description 3
- 230000033228 biological regulation Effects 0.000 description 3
- 239000000872 buffer Substances 0.000 description 3
- 238000009264 composting Methods 0.000 description 3
- 230000005669 field effect Effects 0.000 description 3
- 239000007789 gas Substances 0.000 description 3
- 239000002054 inoculum Substances 0.000 description 3
- 238000011068 loading method Methods 0.000 description 3
- CEQFOVLGLXCDCX-WUKNDPDISA-N methyl red Chemical compound C1=CC(N(C)C)=CC=C1\N=N\C1=CC=CC=C1C(O)=O CEQFOVLGLXCDCX-WUKNDPDISA-N 0.000 description 3
- 244000005700 microbiome Species 0.000 description 3
- 239000006916 nutrient agar Substances 0.000 description 3
- 235000016709 nutrition Nutrition 0.000 description 3
- 230000035764 nutrition Effects 0.000 description 3
- NLKNQRATVPKPDG-UHFFFAOYSA-M potassium iodide Chemical compound [K+].[I-] NLKNQRATVPKPDG-UHFFFAOYSA-M 0.000 description 3
- 239000000047 product Substances 0.000 description 3
- 235000019419 proteases Nutrition 0.000 description 3
- 210000000697 sensory organ Anatomy 0.000 description 3
- 239000000725 suspension Substances 0.000 description 3
- 235000013311 vegetables Nutrition 0.000 description 3
- 239000004246 zinc acetate Substances 0.000 description 3
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 2
- 241000219112 Cucumis Species 0.000 description 2
- 235000015510 Cucumis melo subsp melo Nutrition 0.000 description 2
- 241000196324 Embryophyta Species 0.000 description 2
- CDBYLPFSWZWCQE-UHFFFAOYSA-L Sodium Carbonate Chemical compound [Na+].[Na+].[O-]C([O-])=O CDBYLPFSWZWCQE-UHFFFAOYSA-L 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- FJJCIZWZNKZHII-UHFFFAOYSA-N [4,6-bis(cyanoamino)-1,3,5-triazin-2-yl]cyanamide Chemical compound N#CNC1=NC(NC#N)=NC(NC#N)=N1 FJJCIZWZNKZHII-UHFFFAOYSA-N 0.000 description 2
- DBJUEJCZPKMDPA-UHFFFAOYSA-N acetic acid;zinc Chemical compound [Zn].CC(O)=O DBJUEJCZPKMDPA-UHFFFAOYSA-N 0.000 description 2
- 229910000147 aluminium phosphate Inorganic materials 0.000 description 2
- 230000000844 anti-bacterial effect Effects 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 230000008033 biological extinction Effects 0.000 description 2
- 238000009835 boiling Methods 0.000 description 2
- 238000009395 breeding Methods 0.000 description 2
- 230000001488 breeding effect Effects 0.000 description 2
- 238000004364 calculation method Methods 0.000 description 2
- 235000010980 cellulose Nutrition 0.000 description 2
- 238000010411 cooking Methods 0.000 description 2
- 238000013016 damping Methods 0.000 description 2
- 238000013461 design Methods 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 239000012530 fluid Substances 0.000 description 2
- 239000011521 glass Substances 0.000 description 2
- 229910001385 heavy metal Inorganic materials 0.000 description 2
- 230000005764 inhibitory process Effects 0.000 description 2
- 125000002496 methyl group Chemical group [H]C([H])([H])* 0.000 description 2
- 239000003895 organic fertilizer Substances 0.000 description 2
- 239000010865 sewage Substances 0.000 description 2
- 239000012064 sodium phosphate buffer Substances 0.000 description 2
- 239000007921 spray Substances 0.000 description 2
- 239000012086 standard solution Substances 0.000 description 2
- 230000003068 static effect Effects 0.000 description 2
- 239000006228 supernatant Substances 0.000 description 2
- 239000012085 test solution Substances 0.000 description 2
- 230000009466 transformation Effects 0.000 description 2
- 239000010455 vermiculite Substances 0.000 description 2
- 235000019354 vermiculite Nutrition 0.000 description 2
- 229910052902 vermiculite Inorganic materials 0.000 description 2
- 239000011701 zinc Substances 0.000 description 2
- WHBHBVVOGNECLV-OBQKJFGGSA-N 11-deoxycortisol Chemical compound O=C1CC[C@]2(C)[C@H]3CC[C@](C)([C@@](CC4)(O)C(=O)CO)[C@@H]4[C@@H]3CCC2=C1 WHBHBVVOGNECLV-OBQKJFGGSA-N 0.000 description 1
- 241000244203 Caenorhabditis elegans Species 0.000 description 1
- 240000001980 Cucurbita pepo Species 0.000 description 1
- 235000009852 Cucurbita pepo Nutrition 0.000 description 1
- FBPFZTCFMRRESA-KVTDHHQDSA-N D-Mannitol Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-KVTDHHQDSA-N 0.000 description 1
- 208000035240 Disease Resistance Diseases 0.000 description 1
- 241000305071 Enterobacterales Species 0.000 description 1
- 229930195725 Mannitol Natural products 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- IOVCWXUNBOPUCH-UHFFFAOYSA-M Nitrite anion Chemical compound [O-]N=O IOVCWXUNBOPUCH-UHFFFAOYSA-M 0.000 description 1
- 102000035195 Peptidases Human genes 0.000 description 1
- 239000001888 Peptone Substances 0.000 description 1
- 108010080698 Peptones Proteins 0.000 description 1
- 244000046052 Phaseolus vulgaris Species 0.000 description 1
- 235000010627 Phaseolus vulgaris Nutrition 0.000 description 1
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 1
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
- UISHMWJBGGDOII-UHFFFAOYSA-L S(=S)(=O)([O-])[O-].[Na+].[I+] Chemical compound S(=S)(=O)([O-])[O-].[Na+].[I+] UISHMWJBGGDOII-UHFFFAOYSA-L 0.000 description 1
- DPDMMXDBJGCCQC-UHFFFAOYSA-N [Na].[Cl] Chemical compound [Na].[Cl] DPDMMXDBJGCCQC-UHFFFAOYSA-N 0.000 description 1
- 229960000583 acetic acid Drugs 0.000 description 1
- DPXJVFZANSGRMM-UHFFFAOYSA-N acetic acid;2,3,4,5,6-pentahydroxyhexanal;sodium Chemical compound [Na].CC(O)=O.OCC(O)C(O)C(O)C(O)C=O DPXJVFZANSGRMM-UHFFFAOYSA-N 0.000 description 1
- 239000003929 acidic solution Substances 0.000 description 1
- 238000001632 acidimetric titration Methods 0.000 description 1
- 239000003905 agrochemical Substances 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 229940041514 candida albicans extract Drugs 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 239000001768 carboxy methyl cellulose Substances 0.000 description 1
- 239000005018 casein Substances 0.000 description 1
- BECPQYXYKAMYBN-UHFFFAOYSA-N casein, tech. Chemical compound NCCCCC(C(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(CC(C)C)N=C(O)C(CCC(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(C(C)O)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(COP(O)(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(N)CC1=CC=CC=C1 BECPQYXYKAMYBN-UHFFFAOYSA-N 0.000 description 1
- 235000021240 caseins Nutrition 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 238000002144 chemical decomposition reaction Methods 0.000 description 1
- 235000019219 chocolate Nutrition 0.000 description 1
- 238000002485 combustion reaction Methods 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 230000001186 cumulative effect Effects 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000007865 diluting Methods 0.000 description 1
- LOKCTEFSRHRXRJ-UHFFFAOYSA-I dipotassium trisodium dihydrogen phosphate hydrogen phosphate dichloride Chemical compound P(=O)(O)(O)[O-].[K+].P(=O)(O)([O-])[O-].[Na+].[Na+].[Cl-].[K+].[Cl-].[Na+] LOKCTEFSRHRXRJ-UHFFFAOYSA-I 0.000 description 1
- 230000005611 electricity Effects 0.000 description 1
- 230000008030 elimination Effects 0.000 description 1
- 238000003379 elimination reaction Methods 0.000 description 1
- 238000001952 enzyme assay Methods 0.000 description 1
- 238000012262 fermentative production Methods 0.000 description 1
- 230000035558 fertility Effects 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 230000001408 fungistatic effect Effects 0.000 description 1
- 239000012362 glacial acetic acid Substances 0.000 description 1
- 238000003306 harvesting Methods 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- 239000002655 kraft paper Substances 0.000 description 1
- 239000000594 mannitol Substances 0.000 description 1
- 235000010355 mannitol Nutrition 0.000 description 1
- 238000013507 mapping Methods 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 244000000010 microbial pathogen Species 0.000 description 1
- 229940005654 nitrite ion Drugs 0.000 description 1
- 230000000050 nutritive effect Effects 0.000 description 1
- 230000009965 odorless effect Effects 0.000 description 1
- 210000004681 ovum Anatomy 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 238000012856 packing Methods 0.000 description 1
- 239000000123 paper Substances 0.000 description 1
- 235000019319 peptone Nutrition 0.000 description 1
- 239000002953 phosphate buffered saline Substances 0.000 description 1
- 239000011574 phosphorus Substances 0.000 description 1
- 239000004033 plastic Substances 0.000 description 1
- 235000007715 potassium iodide Nutrition 0.000 description 1
- 229960004839 potassium iodide Drugs 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 239000006041 probiotic Substances 0.000 description 1
- 235000018291 probiotics Nutrition 0.000 description 1
- 235000019833 protease Nutrition 0.000 description 1
- 238000012113 quantitative test Methods 0.000 description 1
- 238000005067 remediation Methods 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- 229910000029 sodium carbonate Inorganic materials 0.000 description 1
- 235000019812 sodium carboxymethyl cellulose Nutrition 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 235000011006 sodium potassium tartrate Nutrition 0.000 description 1
- GEHJYWRUCIMESM-UHFFFAOYSA-L sodium sulfite Chemical compound [Na+].[Na+].[O-]S([O-])=O GEHJYWRUCIMESM-UHFFFAOYSA-L 0.000 description 1
- AKHNMLFCWUSKQB-UHFFFAOYSA-L sodium thiosulfate Chemical compound [Na+].[Na+].[O-]S([O-])(=O)=S AKHNMLFCWUSKQB-UHFFFAOYSA-L 0.000 description 1
- PODWXQQNRWNDGD-UHFFFAOYSA-L sodium thiosulfate pentahydrate Chemical compound O.O.O.O.O.[Na+].[Na+].[O-]S([S-])(=O)=O PODWXQQNRWNDGD-UHFFFAOYSA-L 0.000 description 1
- 235000019345 sodium thiosulphate Nutrition 0.000 description 1
- 230000007480 spreading Effects 0.000 description 1
- 238000003892 spreading Methods 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
- 235000015099 wheat brans Nutrition 0.000 description 1
- 239000012138 yeast extract Substances 0.000 description 1
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
Landscapes
- Fertilizers (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
Description
名称 | 枯草芽孢杆菌 | 细黄链霉菌 | 长柄木霉 | 黑曲霉 | 胶胨样芽孢杆菌 |
(1) | 0.5 | 2.0 | 1.0 | 1.5 | 2.0 |
(2) | 1.0 | 1.5 | 2.0 | 1.0 | 1.5 |
(3) | 1.5 | 1.0 | 1.5 | 0.5 | 1.0 |
(4) | 2.0 | 0.5 | 0.5 | 2.0 | 0.5 |
枯草芽孢杆菌 | 细黄链霉菌 | 长柄木霉 | 黑曲霉 | 胶胨样芽孢杆菌 | |
枯草芽孢杆菌 | - | - | - | - | |
细黄链霉菌 | - | - | - | - | |
长柄木霉 | - | - | - | - | |
黑曲霉 | - | - | - | - | |
胶胨样芽孢杆菌 | - | - | - | - |
试管号 | 标准葡萄糖溶液(ml) | 磷酸钠缓冲液(0.2M pH 6.0)(ml) | 试管中葡萄糖量(μg) |
1 | 0 | 5 | 0 |
2 | 0.4 | 4.6 | 400 |
3 | 0.7 | 4.2 | 800 |
4 | 1.6 | 3.4 | 1600 |
5 | 3.2 | 1.8 | 3200 |
处理 | 纤维素酶活性(u/ml) | 蛋白酶活性(u/ml ) | 淀粉酶活性(u/ mL) |
枯草芽孢杆菌 | 68.00 | 47.20 | 18.90 |
细黄链霉菌 | 12.51 | - | 21.21 |
长柄木霉 | 95.08 | 15.74 | 12.53 |
黑曲霉 | 54.34 | 18.23 | 37.23 |
胶胨样芽孢杆菌 | 15.64 | 8.94 | - |
复合菌剂 | 60.08 | 21.37 | 18.63 |
处理 | 臭气等级 | 氨气含量(10-3mol) | 硫化氢含量(10-3mol) |
枯草芽孢杆菌 | + | 0.00260 | 0.32321 |
细黄链霉菌 | + | 0.00250 | 0.32509 |
长柄木霉 | +++ | 0.00325 | 0.43461 |
黑曲霉 | ++ | 0.00310 | 0.40063 |
胶胨样芽孢杆菌 | ++ | 0.00310 | 0.40098 |
复合菌剂 | + | 0.00222 | 0.30653 |
对照 | ++++ | 0.00713 | 0.65432 |
处理 | 小区平均产量(kg) | 平均亩产量(kg) | 增产(%) |
处理1 | 480 | 3335 | 14.3 |
处理2 | 482 | 3349 | 14.8 |
处理3 | 478 | 3321 | 13.8 |
处理4 | 460 | 3196 | 9.5 |
处理5 | 490 | 3404 | 16.7 |
处理6 | 495 | 3439 | 17.9 |
处理7 | 420 | 2918 | - |
名称 | 农村生活垃圾(%) | 污泥(%) | 玉米秸秆(%) |
(1) | 40 | 40 | 10 |
(2) | 45 | 30 | 15 |
(3) | 50 | 30 | 5 |
(4) | 60 | 20 | 15 |
产量(kg) | 增产幅度 | 增收(元/亩) | 节药(mL) | |
对照 | 5011 | - | - | - |
处理1 | 5564 | - | - | - |
增加 | 553 | 11% | 859 | 200 |
Claims (9)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201410239327.4A CN103966149B (zh) | 2014-06-03 | 2014-06-03 | 一种生活垃圾和污泥腐熟菌剂及其应用和所制腐熟基质 |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201410239327.4A CN103966149B (zh) | 2014-06-03 | 2014-06-03 | 一种生活垃圾和污泥腐熟菌剂及其应用和所制腐熟基质 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN103966149A true CN103966149A (zh) | 2014-08-06 |
CN103966149B CN103966149B (zh) | 2017-11-21 |
Family
ID=51236157
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201410239327.4A Active CN103966149B (zh) | 2014-06-03 | 2014-06-03 | 一种生活垃圾和污泥腐熟菌剂及其应用和所制腐熟基质 |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN103966149B (zh) |
Cited By (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN104193508A (zh) * | 2014-08-14 | 2014-12-10 | 广东省农业科学院果树研究所 | 一种复合有机型环保育苗基质块的制备方法 |
CN104892042A (zh) * | 2015-05-28 | 2015-09-09 | 安顺市西秀区春实绿化苗木有限公司 | 一种生物有机肥的制作工艺 |
CN105801195A (zh) * | 2016-03-09 | 2016-07-27 | 山东植丰农化集团有限公司 | 一种生物有机肥及其制备方法 |
CN107227268A (zh) * | 2017-05-17 | 2017-10-03 | 中国水利水电科学研究院 | 一种秸秆微生物腐熟菌剂及其制备方法 |
CN108402084A (zh) * | 2018-01-24 | 2018-08-17 | 上海环垦生态科技股份有限公司 | 一种防治根部病害的微生物菌剂及制备方法 |
CN109867567A (zh) * | 2019-04-11 | 2019-06-11 | 云南德坤生物科技有限公司 | 一种含钾基小分子碳的腐植酸生物菌肥的制备方法 |
CN109956769A (zh) * | 2019-01-23 | 2019-07-02 | 中鼎特金秦皇岛科技股份有限公司 | 一种秸秆袋式就地堆肥腐熟方法及装置 |
CN110326513A (zh) * | 2019-06-05 | 2019-10-15 | 长江大学 | 一种基于污泥的抗病育苗基质生产方法 |
CN110467272A (zh) * | 2019-08-20 | 2019-11-19 | 陈可 | 一种酶+菌微生物菌剂河道水体与底泥原位修复工艺 |
Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1974492A (zh) * | 2006-12-11 | 2007-06-06 | 陈南岭 | 用垃圾和污泥混合二次发酵生产微生物菌肥的方法 |
CN101294141A (zh) * | 2007-04-28 | 2008-10-29 | 上海四季生物科技有限公司 | 一组用于制备复合微生物肥料的活体微生物制剂及其制作方法 |
CN102690769A (zh) * | 2012-06-01 | 2012-09-26 | 德州市元和农业科技开发有限责任公司 | 一种高效降解粪便的复合微生物菌剂及其制备方法与应用 |
CN103508760A (zh) * | 2013-10-10 | 2014-01-15 | 宣城市南阳生物科技有限公司 | 一种利用鸡粪生产有机肥的方法 |
-
2014
- 2014-06-03 CN CN201410239327.4A patent/CN103966149B/zh active Active
Patent Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1974492A (zh) * | 2006-12-11 | 2007-06-06 | 陈南岭 | 用垃圾和污泥混合二次发酵生产微生物菌肥的方法 |
CN101294141A (zh) * | 2007-04-28 | 2008-10-29 | 上海四季生物科技有限公司 | 一组用于制备复合微生物肥料的活体微生物制剂及其制作方法 |
CN102690769A (zh) * | 2012-06-01 | 2012-09-26 | 德州市元和农业科技开发有限责任公司 | 一种高效降解粪便的复合微生物菌剂及其制备方法与应用 |
CN103508760A (zh) * | 2013-10-10 | 2014-01-15 | 宣城市南阳生物科技有限公司 | 一种利用鸡粪生产有机肥的方法 |
Non-Patent Citations (1)
Title |
---|
李妍等: "污泥堆肥发酵菌剂的筛选及其发酵效果的研究", 《天津农业科学》 * |
Cited By (12)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN104193508A (zh) * | 2014-08-14 | 2014-12-10 | 广东省农业科学院果树研究所 | 一种复合有机型环保育苗基质块的制备方法 |
CN104193508B (zh) * | 2014-08-14 | 2016-06-29 | 广东省农业科学院果树研究所 | 一种复合有机型环保育苗基质块的制备方法 |
CN104892042A (zh) * | 2015-05-28 | 2015-09-09 | 安顺市西秀区春实绿化苗木有限公司 | 一种生物有机肥的制作工艺 |
CN105801195A (zh) * | 2016-03-09 | 2016-07-27 | 山东植丰农化集团有限公司 | 一种生物有机肥及其制备方法 |
CN107227268A (zh) * | 2017-05-17 | 2017-10-03 | 中国水利水电科学研究院 | 一种秸秆微生物腐熟菌剂及其制备方法 |
CN108402084A (zh) * | 2018-01-24 | 2018-08-17 | 上海环垦生态科技股份有限公司 | 一种防治根部病害的微生物菌剂及制备方法 |
CN108402084B (zh) * | 2018-01-24 | 2020-08-11 | 上海环垦生态科技股份有限公司 | 一种防治根部病害的微生物菌剂及制备方法 |
CN109956769A (zh) * | 2019-01-23 | 2019-07-02 | 中鼎特金秦皇岛科技股份有限公司 | 一种秸秆袋式就地堆肥腐熟方法及装置 |
CN109867567A (zh) * | 2019-04-11 | 2019-06-11 | 云南德坤生物科技有限公司 | 一种含钾基小分子碳的腐植酸生物菌肥的制备方法 |
CN110326513A (zh) * | 2019-06-05 | 2019-10-15 | 长江大学 | 一种基于污泥的抗病育苗基质生产方法 |
CN110326513B (zh) * | 2019-06-05 | 2022-04-29 | 长江大学 | 一种基于污泥的抗病育苗基质生产方法 |
CN110467272A (zh) * | 2019-08-20 | 2019-11-19 | 陈可 | 一种酶+菌微生物菌剂河道水体与底泥原位修复工艺 |
Also Published As
Publication number | Publication date |
---|---|
CN103966149B (zh) | 2017-11-21 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN103981107B (zh) | 一种多功能农用微生物菌剂及其使用方法和效果 | |
CN103966150B (zh) | 一种农作物秸秆腐熟菌剂及其使用方法和效果 | |
CN103980019B (zh) | 一种农业固体有机废弃物中温生物发酵工艺及应用 | |
CN103848698B (zh) | 一种利用沼渣好氧发酵制备的生物有机肥及其制备方法 | |
CN103980010B (zh) | 一种畜禽粪便腐熟菌剂及其使用方法和所制生物有机肥 | |
CN103966149A (zh) | 一种生活垃圾和污泥腐熟菌剂及其应用和所制腐熟基质 | |
CN102531766B (zh) | 一种微生物腐熟剂及其生产方法 | |
CN103966148B (zh) | 一种蔬菜废弃物腐熟菌剂及其使用方法和所制有机基质 | |
CN101659934B (zh) | 用于防除连作香蕉巴拿马枯萎病的拮抗菌及其微生物有机肥料 | |
CN103524253B (zh) | 一种育苗基质及其制备方法与用途 | |
CN102199049B (zh) | 一种微生物有机物肥料及其制备方法 | |
CN104211505B (zh) | 生物有机肥及其制备方法 | |
CN103740693B (zh) | 一种秸秆腐熟剂及其制作方法 | |
CN102442849A (zh) | 一种利用秸秆制备的防病生物有机肥及其制备方法 | |
CN106810370A (zh) | 一种含螯合态微量元素的微生物肥料及其制备方法 | |
CN104263677A (zh) | 一种复合微生物拌种剂及其制备方法和应用 | |
CN106068746B (zh) | 一种盐碱地生物改良法 | |
CN106831233B (zh) | 一种利用堆肥产物改良基坑土获得的生物有机土壤及其制备方法 | |
CN106242829A (zh) | 一种克服丹参重茬障碍的土壤修复有机肥、制备方法及其用途 | |
CN106278654A (zh) | 一种克服生姜重茬障碍的土壤修复有机肥、制备方法及其用途 | |
CN108863462A (zh) | 一种以秸秆为原料的生物有机肥及其制备方法 | |
CN106591147A (zh) | 一种黑曲霉njdl‑12菌株及其在滨海盐碱土改良上的应用 | |
CN106045677A (zh) | 一种果树专用的有机复合生物肥的制备方法 | |
CN107298633A (zh) | 一种秸秆堆沤用生物制剂 | |
CN103333851B (zh) | 一种哈茨木霉固体发酵生产分生孢子的方法 |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
C06 | Publication | ||
PB01 | Publication | ||
C10 | Entry into substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
GR01 | Patent grant | ||
GR01 | Patent grant | ||
TR01 | Transfer of patent right |
Effective date of registration: 20210106 Address after: 300192, 26 Nankai District Aerospace Road, Tianjin Patentee after: Tianjin Academy of Agricultural Sciences Address before: 300380 Biological Center building, 17 km away from Jinjing Road, Xiqing District, Tianjin Patentee before: TIANJIN INSTITUTE OF AGRICULTURAL RESOURCES AND ENVIRONMENT SCIENCE |
|
TR01 | Transfer of patent right | ||
TR01 | Transfer of patent right | ||
TR01 | Transfer of patent right |
Effective date of registration: 20231116 Address after: No. 2938 Xiamen Road, Tanggu Ocean Science and Technology Park, Binhai High tech Zone, Binhai New Area, Tianjin, 300000 Patentee after: TIANJIN KUNHE BIOLOGICAL GROUP Co.,Ltd. Address before: 300192, 26 Nankai District Aerospace Road, Tianjin Patentee before: Tianjin Academy of Agricultural Sciences |
|
TR01 | Transfer of patent right | ||
TR01 | Transfer of patent right |
Effective date of registration: 20231206 Address after: 300457 No.202, Huashan Road, Hangu modern industrial zone, Binhai New Area Development Zone, Tianjin Patentee after: TIANJIN DEVELOPMENT ZONE KUNHE BIOTECHNOLOGY Co.,Ltd. Address before: No. 2938 Xiamen Road, Tanggu Ocean Science and Technology Park, Binhai High tech Zone, Binhai New Area, Tianjin, 300000 Patentee before: TIANJIN KUNHE BIOLOGICAL GROUP Co.,Ltd. |
|
TR01 | Transfer of patent right | ||
TR01 | Transfer of patent right |
Effective date of registration: 20240124 Address after: No. 2938 Xiamen Road, Tanggu Ocean Science and Technology Park, Binhai High tech Zone, Binhai New Area, Tianjin, 300000 Patentee after: TIANJIN KUNHE BIOLOGICAL GROUP Co.,Ltd. Country or region after: China Address before: 300457 No.202, Huashan Road, Hangu modern industrial zone, Binhai New Area Development Zone, Tianjin Patentee before: TIANJIN DEVELOPMENT ZONE KUNHE BIOTECHNOLOGY Co.,Ltd. Country or region before: China |