CN103966081B - A kind of negative pressure filter membrane microorganism sampler - Google Patents
A kind of negative pressure filter membrane microorganism sampler Download PDFInfo
- Publication number
- CN103966081B CN103966081B CN201410194515.XA CN201410194515A CN103966081B CN 103966081 B CN103966081 B CN 103966081B CN 201410194515 A CN201410194515 A CN 201410194515A CN 103966081 B CN103966081 B CN 103966081B
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- China
- Prior art keywords
- gas channel
- negative pressure
- filter membrane
- pressure filter
- microorganism
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/12—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by pressure
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/14—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus with filters, sieves or membranes
Abstract
The present invention relates to a kind of negative pressure filter membrane microorganism sampler, the air removal unit comprising sampling thief housing and be arranged on housing, microorganism gathering device, described housing has the gas channel (4) of cruciform four-way pipe structure, gas channel (4) is for cross, the ingress of described gas channel (4) is provided with microorganism gathering device, the intersection or node of gas channel (4) offer outward-dipping opening (11), housing in described opening (11) is provided with air removal unit, this negative pressure filter membrane microorganism sampler compact construction, light-type.Be adapted to the integrated and miniaturization of small space sampling and enviromental monitoring equipment especially.
Description
Technical field
The present invention relates to machinery of sampling equipment technical field, specifically relate to a kind of negative pressure filter membrane microorganism sampler.
Background technology
According to different sampling principles, conventional air microorganism sampler is divided into six classes: 1) impact sampler, comprising solid type, liquid-type and gas-liquid mixed; 2) decanter type sampling thief, comprising natural subsidence formula and heating power decanter type; 3) filtering type sampling thief, comprising solvent-soluble membrane sampling and insolubility filter membrane sampling; 4) electrostatic adhesion sampling thief; 5) Cyclonic sampling thief 6) centrifugal impact sampling thief.Contrast this six classes sampling thief, the volume of filtering type sampling thief is relatively little, but sampling process needs to rely on external air pump to realize collection and the filtration of air, loosely organized and and be difficult to realize lightweight.
Application number is that the Chinese invention patent application of CN201010517630.8 discloses a kind of Miniature personal microbial air sampler, it mainly by: air path part (1), supplying unit (2) and signal processing (3) and mechanical part (4) formed; The sampling head of described air path part have employed micro-filtration membrane device, and filter membrane is positioned at the inside of micro-filtration membrane device.The present invention mainly solves current sampling thief and only uses Alternating Current Power Supply and cause that volume is large, Heavy Weight, and staff carries very inconvenient, the problem that sampling position and environment are very limited.Although this air microorganism sampler have portable, volume is little, the feature such as lightweight, its inlet mouth only has one, and collecting efficiency is lower.In addition, inlet mouth and the air outlet of this air microorganism sampler intersect vertically, and inlet mouth is greater than air outlet again and inlet mouth and air outlet are all one, and this kind of structure design is also unreasonable, is unfavorable for the circulation of air.In addition, the accuracy of change to the sampling inwall that the microorganism in air easily sticks in sampling thief easily changing sampling thief inner air flow velocity and estimation impacts.
In addition, microorganism sampler conventional in prior art is made up of collection device and air pump, loosely organized, and volume is comparatively large, for small space adaptability or be applied to minienvironment monitoring device etc. and there is very large defect.
Summary of the invention
For overcoming above-mentioned shortcomings and deficiencies of the prior art, the invention provides the negative pressure filter membrane air microorganism sampler that a kind of compact construction, light-type and sampling efficiency are high.It is little that this sampling thief has volume, lightweight, compact construction and the higher advantage of collecting efficiency.In pollution incident, on-the-spot or other natural disasteies scenes, can be widely used in miniature self-service environment reconnaissance plane, to the accurate detection of air ambient.For management of public health mechanism reply harmful organism information prevention, intervene and provide technical support in time.
Technical scheme of the present invention is: a kind of negative pressure filter membrane microorganism sampler, comprise sampling thief housing and be arranged on air removal unit, the microorganism gathering device on housing, described housing has the gas channel of cruciform four-way pipe structure, the ingress of described gas channel is provided with microorganism gathering device, the intersection or node of described gas channel offer outward-dipping opening, housing in described opening is provided with air removal unit, and gas channel is cross.Improve the stationarity of air flowing.
Preferably, cross connection place between gas channel and gas channel adopts chamfered transition.This kind is provided with and is beneficial to air and discharges, and improves the acquisition precision of this device.
In above-mentioned either a program preferably, the cross section of gas channel is square.
In above-mentioned either a program preferably, described air removal unit, comprises and is arranged on wind wheel on the turning axle of motor and bracing frame.
In above-mentioned either a program preferably, the turning axle of motor is fixedly connected with wind wheel by interference fit.
In above-mentioned either a program preferably, motor be fixedly mounted on bracing frame collar mounting holes in and be placed in the outside being positioned at gas channel above bracing frame.
In above-mentioned either a program preferably, motor is connected with adopting threaded hole between collar mounting holes by the motor open holes on it.
In above-mentioned either a program preferably, described wind wheel is positioned at the gas channel inside of the below of bracing frame and is in gas channel cross junction place.
In above-mentioned either a program preferably, two external ears of support frame as described above are fixedly connected with by the housing of screw with gas channel top.
In above-mentioned either a program preferably, described motor is direct-current machine.
In above-mentioned either a program preferably, described microorganism gathering device comprises filter screen, millipore filtration and clamping piece.
In above-mentioned either a program preferably, millipore filtration to be attached on filter screen and to be fixedly mounted on the entrance of gas channel by clamping piece.
In above-mentioned either a program preferably, the entrance section of gas channel is square.
In above-mentioned either a program preferably, the entrance left and right sides of gas channel is processed with the first ears flange matched with the ears flange on clamping piece.
In above-mentioned either a program preferably, described clamping piece is square plate with the second ears flange and the central position of square plate offers circular port.
In above-mentioned either a program preferably, described clamping piece is fixedly connected with by the bolt in the connecting hole on the second ears flange with the first ears flange on gas channel entrance and millipore filtration is fixed on the ingress of gas channel.
In above-mentioned either a program preferably, the quantity of described flow channel is at least two.
In above-mentioned either a program preferably, the inner chamber of described gas channel is square gas channel.
In above-mentioned either a program preferably, adjacent between two gas channel is mutually through.
In above-mentioned either a program preferably, the medullary ray of four drift angle medullary rays difference gas channel corresponding thereto of opening overlaps.
In above-mentioned either a program preferably, described wind wheel is multi-blade type wind wheel.
In above-mentioned either a program preferably, sampling thief housing adopts synthetic glass manufacture.
Compared with prior art the invention has the advantages that: the novel microorganism sampling thief of this microorganism sampler compact construction and light-type, to adapt to the integrated and miniaturization of small space sampling and enviromental monitoring equipment.The structural part of sampling thief all adopts synthetic glass to process, and lightweight, volume is little.In addition, the inlet mouth of this sampling thief and the equal diameters of air outlet, air-flow can mild circulation in gas channel, can effectively avoid in background technology owing to adopting device inlet mouth and air outlet diameter is unequal and the generation of " sticky wall " phenomenon and the inaccurate problem of sampling precision that causes occurs.
Main technical principle of the present invention is: utilize the rotation of wind wheel blade to be discharged by the air in space, place, form low pressure area, extraneous air, under the effect of draught head, is discharged after entering into low pressure area by the gas channel being provided with millipore filtration.Microbe granular in air is collected into filter membrane surface.
Accompanying drawing explanation
Fig. 1 is the perspective view of a preferred embodiment of negative pressure filter membrane microorganism sampler of the present invention.
Fig. 2 is the main TV structure schematic diagram embodiment illustrated in fig. 1 of negative pressure filter membrane microorganism sampler of the present invention.
Fig. 3 is the plan structure schematic diagram embodiment illustrated in fig. 2 of negative pressure filter membrane microorganism sampler of the present invention.
In figure, Reference numeral is: 1: motor, 2: bracing frame, 3: wind wheel, 4: gas channel, 5: inclined-plane, 6: the second ears flanges 7: clamping piece, 8: filter screen, 9: millipore filtration, 10: circular port, 11: opening.
Embodiment
Below in conjunction with accompanying drawing, explanation is further elaborated to the preferred embodiments of the present invention;
Embodiment 1:
As shown in Figure 1, a kind of negative pressure filter membrane microorganism sampler, comprise sampling thief housing and be arranged on air removal unit, the microorganism gathering device on housing, described housing is integral type structure.Described housing has the four-way pipe structure of cruciform gas channel 4.Gas channel 4 in described housing is right-angled intersection, and the housing of the infall of gas channel 4 offers opening 11.Further in the intersection or node of gas channel 4, offer outward-dipping opening 11.Be provided with air removal unit on the housing, described air removal unit is positioned at directly over opening 11.Microorganism gathering device is installed with in the ingress of described gas channel 4.
Cross connection place employing inclined-plane 5 transition between gas channel 4 and gas channel 4, employing inclined-plane 5 carries out the gas uniform speed walking that transition is conducive to entering in gas channel 4.The cross section of gas channel 4 is square.Described air removal unit is arranged on the opening 11 above the intersection of gas channel 4 and gas channel or node.Flow channel 4 is in the present embodiment four and the inner chamber of described gas channel 4 is square gas channel, and gas channel 4 adjacent is between two mutually through and form chamber in described housing.Opening 11 is set to square equally, and the medullary ray of four drift angle medullary rays difference each gas channel 4 corresponding thereto of opening 11 overlaps.
As shown in figs. 1 and 3, described air removal unit comprises the wind wheel 3 on the turning axle being arranged on motor 1, and described wind wheel 3 is multi-blade type wind wheel.The turning axle of motor 1 is fixedly connected with wind wheel 3 by interference fit, and motor 1 is fixedly connected with described housing by bracing frame 2.In the present embodiment, motor 1 be fixedly mounted on bracing frame 2 collar mounting holes in and be placed in the outside being positioned at gas channel 4 above bracing frame 2.Motor 1 is connected with adopting threaded hole between collar mounting holes by the motor open holes on it.When the turning axle rotation of motor 1 drives wind wheel 3 to rotate, in described housing, form negative pressuren zone, the air forcing the entrance being positioned at gas channel 4 to go out enters gas channel, and is discharged outside described housing by air through the effect of wind wheel 3.
Wind wheel 3 is positioned at gas channel 4 inside of the below of bracing frame 2 and is in gas channel 4 cross junction place.Two external ears of bracing frame 2 by screw to be fixedly connected with the housing on gas channel 4 top by described motor firmly fixing on the housing.In the present embodiment, what motor 1 adopted is direct-current machine.
As Figure 1-3, be provided with microorganism gathering device in the ingress of the gas channel 4 of described housing, this device comprises filter screen 8, millipore filtration 9 and clamping piece 7.Millipore filtration 9 to be attached on filter screen 8 and to be fixedly mounted on by clamping piece 7 on the entrance of gas channel 4.The shape of filter screen 8 and the shape approximation of gas channel 4.Entrance section due to gas channel 4 is square.So filter screen 8 also adopts the structural shape matched with gas channel entrance shape.
Described clamping piece 7 is square plate with the second ears flange 6 and the central position of square plate offers circular port 10.By described millipore filtration 9 docile in the outside of filter screen 8, then by clamping piece 7, the combination both it is fixed on the entrance of gas channel 4.The diameter of circular port 10 is less than the diameter of gas channel 4, and the object that this kind is arranged is, when extraneous air is by after circular port 10, can to enter stably in gas channel 4 and to get rid of outside described housing.
The the first ears flange matched with the second ears flange 6 on clamping piece 7 is processed with in the entrance left and right sides of gas channel 4.Described clamping piece 7 is fixedly connected with by the bolt in the connecting hole 11 on the second ears flange 6 with the first ears flange on gas channel 4 entrance and millipore filtration 9 is fixed on the ingress of gas channel 4.In the present embodiment, negative pressure filter membrane microorganism sampler housing adopts synthetic glass manufacture.The housing of synthetic glass manufacture is adopted to have lightweight, the advantages such as volume is little.
Main technical principle of the present invention is: utilize the blade of wind wheel 3 to be discharged by the air of (cavity that gas channel and gas channel are formed) in space, place in the process rotated, form low pressure area or negative pressuren zone, extraneous air, due under the effect of draught head, is discharged after entering into low pressure area discharged by opening 11 by the gas channel being provided with millipore filtration.Microbe granular in air can be collected on filter membrane surface naturally, forms the object gathering microbes in air.
Embodiment 2: be that gas channel 4 is columnar structured with embodiment 1 difference in the present embodiment.Microorganism gathering device to be screwed with gas channel 4 by screw thread and is connected.
It is little, lightweight that negative pressure filter membrane microorganism sampler of the present invention has volume relative to the sampling thief in background technology, compact construction and the higher advantage of collecting efficiency.Particularly or other natural disasteies scenes on-the-spot in pollution incident, can be widely used in miniature self-service environment reconnaissance plane, to the accurate detection of air ambient.For management of public health mechanism reply harmful organism information prevention, intervene and provide technical support in time.In addition, the inlet mouth of this sampling thief and the equal diameters of air outlet, air-flow can mild circulation in gas channel, can effectively avoid in background technology owing to adopting device inlet mouth and air outlet diameter is unequal and the generation of " sticky wall " phenomenon and the inaccurate problem of sampling precision that causes occurs.
It should be noted that, comprise the arbitrary combination between each part mentioned above according to the category of the technical scheme of negative pressure filter membrane microorganism sampler of the present invention.More than be described in detail in conjunction with specific embodiments of the invention, but be not limitation of the present invention, everyly according to technical spirit of the present invention, technical scope of the present invention is all belonged to any simple modification made for any of the above embodiments.
Claims (16)
1. a negative pressure filter membrane microorganism sampler, comprise sampling thief housing and be arranged on air removal unit, the microorganism gathering device on housing, it is characterized in that: described housing has the gas channel of cruciform four-way pipe structure, gas channel is cross, the ingress of described gas channel is provided with microorganism gathering device, the intersection or node of gas channel offer outward-dipping opening, the housing in described opening is provided with air removal unit; Described air removal unit, comprises and is arranged on wind wheel on the turning axle of motor and bracing frame; Be placed in the outside being positioned at gas channel above bracing frame in the collar mounting holes that motor is fixedly mounted on bracing frame.
2. negative pressure filter membrane microorganism sampler as claimed in claim 1, is characterized in that: the cross section of gas channel is square.
3. negative pressure filter membrane microorganism sampler as claimed in claim 1, is characterized in that: the turning axle of motor is fixedly connected with wind wheel by interference fit.
4. negative pressure filter membrane microorganism sampler as claimed in claim 1, is characterized in that: motor is connected with adopting threaded hole between collar mounting holes by the motor open holes on it.
5. negative pressure filter membrane microorganism sampler as claimed in claim 3, is characterized in that: wind wheel is positioned at the gas channel inside of the below of bracing frame and is in gas channel cross junction place.
6. negative pressure filter membrane microorganism sampler as claimed in claim 1, is characterized in that: two external ears of bracing frame are fixedly connected with by the housing of screw with gas channel top.
7. negative pressure filter membrane microorganism sampler as claimed in claim 1, is characterized in that: described motor is direct-current machine.
8. negative pressure filter membrane microorganism sampler as claimed in claim 1, is characterized in that: described microorganism gathering device comprises filter screen, millipore filtration and clamping piece.
9. negative pressure filter membrane microorganism sampler as claimed in claim 8, is characterized in that: millipore filtration to be attached on filter screen and to be fixedly mounted on the entrance of gas channel by clamping piece.
10. negative pressure filter membrane microorganism sampler as claimed in claim 9, is characterized in that: the entrance section of gas channel is square.
11. negative pressure filter membrane microorganism samplers as claimed in claim 10, is characterized in that: described clamping piece is square plate with the second ears flange and the central position of square plate offers circular port.
12. negative pressure filter membrane microorganism samplers as claimed in claim 11, is characterized in that: the entrance left and right sides of gas channel is processed with the first ears flange matched with the second ears flange on clamping piece.
13. negative pressure filter membrane microorganism samplers as claimed in claim 12, is characterized in that: described clamping piece is fixedly connected with by the bolt in the connecting hole on the second ears flange with the first ears flange on gas channel entrance and millipore filtration is fixed on the ingress of gas channel.
14. negative pressure filter membrane microorganism samplers as claimed in claim 13, is characterized in that: the inner chamber of described gas channel is square gas channel.
15. negative pressure filter membrane microorganism samplers as claimed in claim 1, is characterized in that: described wind wheel is multi-blade type wind wheel.
16. negative pressure filter membrane microorganism samplers as claimed in claim 1, is characterized in that: sampling thief housing adopts synthetic glass manufacture.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410194515.XA CN103966081B (en) | 2014-05-09 | 2014-05-09 | A kind of negative pressure filter membrane microorganism sampler |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410194515.XA CN103966081B (en) | 2014-05-09 | 2014-05-09 | A kind of negative pressure filter membrane microorganism sampler |
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| Publication Number | Publication Date |
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| CN103966081A CN103966081A (en) | 2014-08-06 |
| CN103966081B true CN103966081B (en) | 2015-12-09 |
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| CN201410194515.XA Expired - Fee Related CN103966081B (en) | 2014-05-09 | 2014-05-09 | A kind of negative pressure filter membrane microorganism sampler |
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Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106969935B (en) * | 2017-02-23 | 2019-04-16 | 河海大学 | A kind of aquatic organism film acquisition system and acquisition method |
| CN113049319A (en) * | 2021-03-09 | 2021-06-29 | 上海交通大学 | Negative pressure entering chamber type filter membrane aerosol microorganism sampler |
| CN114045217B (en) * | 2022-01-11 | 2022-04-19 | 至美时代生物智能科技(北京)有限公司 | Air microorganism sampling device and air microorganism sampling and detecting integrated device |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2352949Y (en) * | 1998-11-16 | 1999-12-08 | 李玉国 | Large flow rate atmosphere sampler |
| CN101974418A (en) * | 2010-10-24 | 2011-02-16 | 青岛众瑞智能仪器有限公司 | Miniature personal microbial air sampler |
| CN103776666A (en) * | 2014-02-17 | 2014-05-07 | 青岛众瑞智能仪器有限公司 | Six-channel air particle sampling device |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8012226B2 (en) * | 2007-07-17 | 2011-09-06 | Ging-Chung Chen | Air separator and purifier device |
| US7704293B2 (en) * | 2007-10-23 | 2010-04-27 | Institute Of Nuclear Energy Research | Turbulence device used for air filtration system |
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2014
- 2014-05-09 CN CN201410194515.XA patent/CN103966081B/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2352949Y (en) * | 1998-11-16 | 1999-12-08 | 李玉国 | Large flow rate atmosphere sampler |
| CN101974418A (en) * | 2010-10-24 | 2011-02-16 | 青岛众瑞智能仪器有限公司 | Miniature personal microbial air sampler |
| CN103776666A (en) * | 2014-02-17 | 2014-05-07 | 青岛众瑞智能仪器有限公司 | Six-channel air particle sampling device |
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Granted publication date: 20151209 Termination date: 20180509 |