CN103954776B - Ngal光激发化学发光检测试剂盒、其制备及使用方法 - Google Patents
Ngal光激发化学发光检测试剂盒、其制备及使用方法 Download PDFInfo
- Publication number
- CN103954776B CN103954776B CN201410197672.6A CN201410197672A CN103954776B CN 103954776 B CN103954776 B CN 103954776B CN 201410197672 A CN201410197672 A CN 201410197672A CN 103954776 B CN103954776 B CN 103954776B
- Authority
- CN
- China
- Prior art keywords
- microballoon
- ngal
- antibody
- luminous
- optical excitation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 102000013519 Lipocalin-2 Human genes 0.000 title claims abstract description 33
- 108010051335 Lipocalin-2 Proteins 0.000 title claims abstract description 33
- 238000000034 method Methods 0.000 title claims abstract description 24
- 230000003287 optical effect Effects 0.000 title claims abstract description 16
- 230000005284 excitation Effects 0.000 title claims abstract description 15
- 238000002038 chemiluminescence detection Methods 0.000 title claims abstract description 14
- 238000002360 preparation method Methods 0.000 title abstract description 9
- 238000010168 coupling process Methods 0.000 claims abstract description 30
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 claims abstract description 28
- 230000008878 coupling Effects 0.000 claims abstract description 28
- 238000005859 coupling reaction Methods 0.000 claims abstract description 28
- 239000007853 buffer solution Substances 0.000 claims abstract description 22
- 206010070834 Sensitisation Diseases 0.000 claims abstract description 20
- 230000008313 sensitization Effects 0.000 claims abstract description 20
- 235000020958 biotin Nutrition 0.000 claims abstract description 16
- 239000000243 solution Substances 0.000 claims abstract description 16
- 108090001008 Avidin Proteins 0.000 claims abstract description 14
- 229960002685 biotin Drugs 0.000 claims abstract description 14
- 239000011616 biotin Substances 0.000 claims abstract description 14
- 238000006243 chemical reaction Methods 0.000 claims abstract description 9
- MWPLVEDNUUSJAV-UHFFFAOYSA-N anthracene Chemical compound C1=CC=CC2=CC3=CC=CC=C3C=C21 MWPLVEDNUUSJAV-UHFFFAOYSA-N 0.000 claims abstract description 8
- 150000001718 carbodiimides Chemical class 0.000 claims abstract description 7
- 230000004913 activation Effects 0.000 claims abstract description 6
- 238000002372 labelling Methods 0.000 claims abstract description 6
- 125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 claims abstract description 5
- 229910052693 Europium Inorganic materials 0.000 claims abstract description 4
- 239000013522 chelant Substances 0.000 claims abstract description 4
- OGPBJKLSAFTDLK-UHFFFAOYSA-N europium atom Chemical compound [Eu] OGPBJKLSAFTDLK-UHFFFAOYSA-N 0.000 claims abstract description 4
- YYMBJDOZVAITBP-UHFFFAOYSA-N rubrene Chemical compound C1=CC=CC=C1C(C1=C(C=2C=CC=CC=2)C2=CC=CC=C2C(C=2C=CC=CC=2)=C11)=C(C=CC=C2)C2=C1C1=CC=CC=C1 YYMBJDOZVAITBP-UHFFFAOYSA-N 0.000 claims abstract description 4
- RPENMORRBUTCPR-UHFFFAOYSA-M sodium;1-hydroxy-2,5-dioxopyrrolidine-3-sulfonate Chemical compound [Na+].ON1C(=O)CC(S([O-])(=O)=O)C1=O RPENMORRBUTCPR-UHFFFAOYSA-M 0.000 claims abstract 2
- 230000009514 concussion Effects 0.000 claims description 6
- 239000001007 phthalocyanine dye Substances 0.000 claims description 3
- 241001614291 Anoplistes Species 0.000 claims 1
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims 1
- 229910052799 carbon Inorganic materials 0.000 claims 1
- 238000001514 detection method Methods 0.000 abstract description 23
- 230000035945 sensitivity Effects 0.000 abstract description 9
- 230000007613 environmental effect Effects 0.000 abstract description 4
- 239000006228 supernatant Substances 0.000 description 13
- 239000003153 chemical reaction reagent Substances 0.000 description 11
- 238000010790 dilution Methods 0.000 description 9
- 239000012895 dilution Substances 0.000 description 9
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 description 6
- 229920001213 Polysorbate 20 Polymers 0.000 description 6
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 6
- 229910052760 oxygen Inorganic materials 0.000 description 6
- 239000001301 oxygen Substances 0.000 description 6
- 235000010486 polyoxyethylene sorbitan monolaurate Nutrition 0.000 description 6
- 239000000256 polyoxyethylene sorbitan monolaurate Substances 0.000 description 6
- 210000002966 serum Anatomy 0.000 description 6
- 150000003626 triacylglycerols Chemical class 0.000 description 6
- 238000005406 washing Methods 0.000 description 6
- 102000001554 Hemoglobins Human genes 0.000 description 5
- 108010054147 Hemoglobins Proteins 0.000 description 5
- 238000004458 analytical method Methods 0.000 description 5
- 230000006287 biotinylation Effects 0.000 description 5
- 238000007413 biotinylation Methods 0.000 description 5
- 239000000872 buffer Substances 0.000 description 5
- 238000011534 incubation Methods 0.000 description 5
- BPYKTIZUTYGOLE-IFADSCNNSA-N Bilirubin Chemical compound N1C(=O)C(C)=C(C=C)\C1=C\C1=C(C)C(CCC(O)=O)=C(CC2=C(C(C)=C(\C=C/3C(=C(C=C)C(=O)N\3)C)N2)CCC(O)=O)N1 BPYKTIZUTYGOLE-IFADSCNNSA-N 0.000 description 4
- 239000003795 chemical substances by application Substances 0.000 description 3
- 238000002474 experimental method Methods 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- BTBUEUYNUDRHOZ-UHFFFAOYSA-N Borate Chemical compound [O-]B([O-])[O-] BTBUEUYNUDRHOZ-UHFFFAOYSA-N 0.000 description 2
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 2
- 102000019298 Lipocalin Human genes 0.000 description 2
- 108050006654 Lipocalin Proteins 0.000 description 2
- 239000007987 MES buffer Substances 0.000 description 2
- ZYBXCIBWFOILPE-UHFFFAOYSA-N [Na].N#CC#N Chemical compound [Na].N#CC#N ZYBXCIBWFOILPE-UHFFFAOYSA-N 0.000 description 2
- 150000001615 biotins Chemical class 0.000 description 2
- 238000011088 calibration curve Methods 0.000 description 2
- BVKZGUZCCUSVTD-UHFFFAOYSA-N carbonic acid Chemical compound OC(O)=O BVKZGUZCCUSVTD-UHFFFAOYSA-N 0.000 description 2
- 150000001875 compounds Chemical class 0.000 description 2
- CCIVGXIOQKPBKL-UHFFFAOYSA-N ethanesulfonic acid Chemical compound CCS(O)(=O)=O CCIVGXIOQKPBKL-UHFFFAOYSA-N 0.000 description 2
- 125000002485 formyl group Chemical class [H]C(*)=O 0.000 description 2
- 238000006197 hydroboration reaction Methods 0.000 description 2
- 238000003018 immunoassay Methods 0.000 description 2
- 239000008363 phosphate buffer Substances 0.000 description 2
- 239000000047 product Substances 0.000 description 2
- 238000003908 quality control method Methods 0.000 description 2
- 230000035484 reaction time Effects 0.000 description 2
- 239000012086 standard solution Substances 0.000 description 2
- 239000000725 suspension Substances 0.000 description 2
- 238000000108 ultra-filtration Methods 0.000 description 2
- NQRKYASMKDDGHT-UHFFFAOYSA-N (aminooxy)acetic acid Chemical compound NOCC(O)=O NQRKYASMKDDGHT-UHFFFAOYSA-N 0.000 description 1
- 102000013382 Gelatinases Human genes 0.000 description 1
- 108010026132 Gelatinases Proteins 0.000 description 1
- 206010018910 Haemolysis Diseases 0.000 description 1
- 230000000975 bioactive effect Effects 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 230000003139 buffering effect Effects 0.000 description 1
- -1 carboxymethoxyl Chemical group 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 239000000084 colloidal system Substances 0.000 description 1
- 230000002596 correlated effect Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000009792 diffusion process Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000006870 function Effects 0.000 description 1
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 1
- 239000010931 gold Substances 0.000 description 1
- 229910052737 gold Inorganic materials 0.000 description 1
- 230000008588 hemolysis Effects 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- HVHKMUMXERBUAN-IFADSCNNSA-N mesobilirubin IXalpha Chemical compound N1C(=O)C(CC)=C(C)\C1=C\C1=C(C)C(CCC(O)=O)=C(CC2=C(C(C)=C(\C=C/3C(=C(C)C(=O)N\3)CC)N2)CCC(O)=O)N1 HVHKMUMXERBUAN-IFADSCNNSA-N 0.000 description 1
- 230000007935 neutral effect Effects 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 239000002504 physiological saline solution Substances 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000004445 quantitative analysis Methods 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 239000012266 salt solution Substances 0.000 description 1
- 230000001235 sensitizing effect Effects 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/531—Production of immunochemical test materials
- G01N33/532—Production of labelled immunochemicals
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54393—Improving reaction conditions or stability, e.g. by coating or irradiation of surface, by reduction of non-specific binding, by promotion of specific binding
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/92—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving lipids, e.g. cholesterol, lipoproteins, or their receptors
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Immunology (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Chemical & Material Sciences (AREA)
- Biomedical Technology (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Food Science & Technology (AREA)
- Biochemistry (AREA)
- Cell Biology (AREA)
- Biotechnology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Biophysics (AREA)
- Endocrinology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Peptides Or Proteins (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
Abstract
Description
次数 | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | MEAN | SD |
盐水 | 1720 | 1667 | 1682 | 1700 | 1688 | 1677 | 1669 | 1711 | 1691 | 1680 | 1688.5 | 17.40 |
样本 | 25866 | 25855 | 25766 | 25780 | 25797 | 25831 | 25877 | 25785 | 25765 | 25879 | 25820.1 | 46.53 |
Claims (6)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201410197672.6A CN103954776B (zh) | 2014-05-12 | 2014-05-12 | Ngal光激发化学发光检测试剂盒、其制备及使用方法 |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201410197672.6A CN103954776B (zh) | 2014-05-12 | 2014-05-12 | Ngal光激发化学发光检测试剂盒、其制备及使用方法 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN103954776A CN103954776A (zh) | 2014-07-30 |
CN103954776B true CN103954776B (zh) | 2016-05-18 |
Family
ID=51332074
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201410197672.6A Active CN103954776B (zh) | 2014-05-12 | 2014-05-12 | Ngal光激发化学发光检测试剂盒、其制备及使用方法 |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN103954776B (zh) |
Families Citing this family (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN116337851A (zh) * | 2018-07-18 | 2023-06-27 | 科美博阳诊断技术(上海)有限公司 | 一种均相化学发光检测试剂盒及其应用 |
CN109633163B (zh) * | 2018-11-28 | 2022-03-04 | 浙江聚康生物工程有限公司 | 降钙素原/c反应蛋白二合一检测试剂盒 |
CN111665235A (zh) * | 2019-03-08 | 2020-09-15 | 上海索昕生物科技有限公司 | 一种化学发光微阵列芯片及其应用 |
CN110907636A (zh) * | 2019-12-17 | 2020-03-24 | 天津森郁生物科技有限公司 | 一种光激化学发光均相免疫分析方法 |
CN111077304A (zh) * | 2019-12-31 | 2020-04-28 | 深圳市瀚德标检生物工程有限公司 | 一种羧基乳胶微球与抗体的偶联方法 |
CN113376146A (zh) * | 2020-02-25 | 2021-09-10 | 上海交通大学 | 适于生物分子多重检测的检测颗粒及其制备方法与应用 |
CN111579801B (zh) * | 2020-06-02 | 2024-01-30 | 苏州翊讯生物科技有限公司 | 用于抗缪勒氏管激素含量检测的单人份试剂盒及其检测方法 |
Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6251581B1 (en) * | 1991-05-22 | 2001-06-26 | Dade Behring Marburg Gmbh | Assay method utilizing induced luminescence |
CN101281137A (zh) * | 2008-04-24 | 2008-10-08 | 博阳生物科技(上海)有限公司 | 光激化学发光免疫检测方法 |
CN102967714A (zh) * | 2012-12-10 | 2013-03-13 | 天津市协和医药科技集团有限公司 | 中性粒细胞明胶酶相关脂质运载蛋白化学发光检测试剂盒 |
Family Cites Families (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1791797A (zh) * | 2003-03-27 | 2006-06-21 | 儿童医院医疗中心 | 用于检测肾小管细胞损伤的早发的方法和试剂盒 |
CN102253217B (zh) * | 2011-04-07 | 2013-08-07 | 武汉生之源生物科技有限公司 | 一种胶乳颗粒增强型中性粒细胞明胶酶相关脂质运载蛋白检测试剂盒 |
CN102680698B (zh) * | 2011-07-29 | 2016-03-30 | 南京诺尔曼生物技术有限公司 | 中性粒细胞明胶酶相关脂质运载蛋白(ngal)测定试剂盒(胶乳增强免疫比浊法) |
-
2014
- 2014-05-12 CN CN201410197672.6A patent/CN103954776B/zh active Active
Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6251581B1 (en) * | 1991-05-22 | 2001-06-26 | Dade Behring Marburg Gmbh | Assay method utilizing induced luminescence |
CN101281137A (zh) * | 2008-04-24 | 2008-10-08 | 博阳生物科技(上海)有限公司 | 光激化学发光免疫检测方法 |
CN102967714A (zh) * | 2012-12-10 | 2013-03-13 | 天津市协和医药科技集团有限公司 | 中性粒细胞明胶酶相关脂质运载蛋白化学发光检测试剂盒 |
Non-Patent Citations (6)
Title |
---|
Luminescent oxygen channeling assay sensitive, broadly applicable homogeneous immunoassay method;Edwin F. ULLMAN;《Clinical Chemistry》;19961231;第42卷;第1518-1526页 * |
Luminescent oxygen channeling immunoassay: Masurement of particle binding kinetics by chemiluminescence;EDWIN F. ULLMAN;《PNAS》;19940630;第91卷;第5426-5430页 * |
中性粒细胞明胶酶相关脂质运载蛋白的研究进展;须静;《检验医学》;20121031;第27卷(第10期);第871-875页 * |
光激化学发光均相免疫分析的应用动态;范少娟 等;《纳米技术与精密工程》;20140331;第12卷(第2期);第151-155页 * |
光激化学发光技术研究进展与应用;高云朝;《中华医学检验杂志》;20090430;第32卷(第4期);第474-478页 * |
均相光激化学发光免疫分析技术的研究进展;张存亮 等;《中国动物检疫》;20140331;第31卷(第3期);第46-50页 * |
Also Published As
Publication number | Publication date |
---|---|
CN103954776A (zh) | 2014-07-30 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN103954776B (zh) | Ngal光激发化学发光检测试剂盒、其制备及使用方法 | |
CN102662061B (zh) | 胶乳增强免疫比浊法测定人甲胎蛋白含量的试剂盒 | |
CN103443626B (zh) | 链霉亲和素结合磁性粒子及其制造方法 | |
CN107817232A (zh) | 用于进行过敏症和自身免疫性疾病的诊断测定的自动化免疫分析系统 | |
CN104849469A (zh) | 一种检测ngal含量的试剂盒及其制备方法 | |
CN104246500A (zh) | 宽范围发光免疫分析 | |
CN103823070A (zh) | 一种高灵敏度的胱抑素c测定试剂盒 | |
CN103954766B (zh) | 转铁蛋白受体检测试剂盒及制备方法 | |
CN102692504A (zh) | D-二聚体荧光免疫定量测定试纸条及其制备方法 | |
CN107918022B (zh) | 一种cTnI检测试剂盒及其使用方法 | |
CN101949942A (zh) | 游离三碘甲状腺原氨酸定量检测试剂盒及其制备方法 | |
CN107807241A (zh) | 一种血管内表皮生长因子受体1的磁微粒化学发光检测试剂盒及制备方法 | |
JP6820319B2 (ja) | 増強された感度を有するイムノアッセイ | |
CN104535770A (zh) | 一种复合抗体的肌红蛋白测定试剂盒 | |
CN105388292A (zh) | 试剂盒及联合检测pct、crp及il-6的方法 | |
CN104614528A (zh) | 一种线性范围更宽的视黄醇结合蛋白测定试剂盒 | |
CN106093411A (zh) | 一种一步均相ck‑mb检测试剂盒及其应用 | |
CN107957495A (zh) | 一种ck-mb检测试剂盒及其使用方法 | |
CN104535761B (zh) | 超支化聚缩水甘油醚修饰胶乳微球增强免疫比浊法及其应用 | |
CN108872616A (zh) | 基于单粒径胶乳颗粒的检测ngal的免疫胶乳比浊法试剂盒 | |
Ghosh et al. | Detection of c-reactive protein using network-deployable DNA aptamer based optical nanosensor | |
CN103529225B (zh) | 一种肝型脂肪酸结合蛋白含量检测试剂盒及其制备方法 | |
CN106124771A (zh) | 一种一步均相cTnT检测试剂盒及其应用 | |
CN107942072A (zh) | 肾损伤因子1检测试剂盒 | |
CN104483492A (zh) | 抗链球菌溶血素o抗体的检测试剂盒 |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
C06 | Publication | ||
PB01 | Publication | ||
C10 | Entry into substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
C41 | Transfer of patent application or patent right or utility model | ||
TA01 | Transfer of patent application right |
Effective date of registration: 20160331 Address after: 430206 hi tech medical equipment Park No. 818, hi tech Avenue, East Lake Development Zone, Wuhan, Hubei, Wuhan, B11 Applicant after: Wuhan Life Origin Biotech Co., Ltd. Address before: 430206 hi tech medical equipment Park No. 818, hi tech Avenue, East Lake Development Zone, Wuhan, Hubei, Wuhan, B11 Applicant before: Hua Quangao |
|
C14 | Grant of patent or utility model | ||
GR01 | Patent grant | ||
C56 | Change in the name or address of the patentee | ||
CP03 | Change of name, title or address |
Address after: 430206 B11 building, No. 818, hi tech medical center, hi tech Avenue, East Lake Development Zone, Wuhan, Hubei, 2 Patentee after: WUHAN LIFE ORIGIN BIOTECH JOINT STOCK CO., LTD. Address before: 430206 hi tech medical equipment Park No. 818, hi tech Avenue, East Lake Development Zone, Wuhan, Hubei, Wuhan, B11 Patentee before: Wuhan Life Origin Biotech Co., Ltd. |
|
PE01 | Entry into force of the registration of the contract for pledge of patent right | ||
PE01 | Entry into force of the registration of the contract for pledge of patent right |
Denomination of invention: NGAL (Neutrophil Gelatinase Associated Lipocalin) optical excitation chemiluminescence detection kit and preparation and use methods of kit Effective date of registration: 20180612 Granted publication date: 20160518 Pledgee: Wuhan rural commercial bank Limited by Share Ltd Optics Valley branch Pledgor: WUHAN LIFE ORIGIN BIOTECH JOINT STOCK CO., LTD. Registration number: 2018420000024 |
|
PC01 | Cancellation of the registration of the contract for pledge of patent right | ||
PC01 | Cancellation of the registration of the contract for pledge of patent right |
Date of cancellation: 20190620 Granted publication date: 20160518 Pledgee: Wuhan rural commercial bank Limited by Share Ltd Optics Valley branch Pledgor: WUHAN LIFE ORIGIN BIOTECH JOINT STOCK CO., LTD. Registration number: 2018420000024 |
|
PE01 | Entry into force of the registration of the contract for pledge of patent right | ||
PE01 | Entry into force of the registration of the contract for pledge of patent right |
Denomination of invention: NGAL (Neutrophil Gelatinase Associated Lipocalin) optical excitation chemiluminescence detection kit and preparation and use methods of kit Effective date of registration: 20191107 Granted publication date: 20160518 Pledgee: Guanggu Branch of Wuhan Rural Commercial Bank Co., Ltd. Pledgor: WUHAN LIFE ORIGIN BIOTECH JOINT STOCK CO., LTD. Registration number: Y2019420000025 |
|
PC01 | Cancellation of the registration of the contract for pledge of patent right | ||
PC01 | Cancellation of the registration of the contract for pledge of patent right |
Date of cancellation: 20201223 Granted publication date: 20160518 Pledgee: Guanggu Branch of Wuhan Rural Commercial Bank Co.,Ltd. Pledgor: WUHAN LIFE ORIGIN BIOTECH JOINT STOCK Co.,Ltd. Registration number: Y2019420000025 |
|
CP02 | Change in the address of a patent holder | ||
CP02 | Change in the address of a patent holder |
Address after: 430000 1 / F, 2 / F and 3 / F, B11, hi tech medical device Park, 818 Gaoxin Avenue, Donghu Development Zone, Wuhan, Hubei Province Patentee after: WUHAN LIFE ORIGIN BIOTECH JOINT STOCK Co.,Ltd. Address before: 2 / F, B11, Gaoke medical equipment Park, 818 Gaoxin Avenue, Donghu Development Zone, Wuhan Patentee before: WUHAN LIFE ORIGIN BIOTECH JOINT STOCK Co.,Ltd. |