CN103951504A - Preparation method of culture medium for promoting rapid growth of enoki mushroom mycelium - Google Patents
Preparation method of culture medium for promoting rapid growth of enoki mushroom mycelium Download PDFInfo
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- CN103951504A CN103951504A CN201410159572.4A CN201410159572A CN103951504A CN 103951504 A CN103951504 A CN 103951504A CN 201410159572 A CN201410159572 A CN 201410159572A CN 103951504 A CN103951504 A CN 103951504A
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- putrescine
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Abstract
The invention discloses a preparation method of a culture medium for promoting the rapid growth of enoki mushroom mycelium, which belongs to the technical field of edible fungus cultivation. The method comprises the following steps of mixing putrescine with distilled water so as to obtain a putrescine aqueous solution with a concentration of 10 mg/ml; uniformly stirring bran, cotton seed shells, rice bran, the putrescine aqueous solution and water so as to obtain a culture medium mixture; repackaging the culture medium mixture into cultivation bottles, after the cultivation bottles are sealed, putting the cultivation bottles at an environment temperature of 95-105 DEG C to carry out sterilization processing, and then naturally cooling the cultivation bottles, so that the culture medium for promoting the rapid growth of enoki mushroom mycelium is obtained. According to the invention, on the basis of guaranteeing the essential nutrients for the growth and reproduction of enoki mushrooms, the culture medium is simplified, and the cost of raw materials is reduced; necessary nutrients are provided for the growth and reproduction of enoki mushrooms, the cotton seed shells provide a suitable growth environment, and the putrescine strengthens the division growth of cells, and shortens the production cycle, thereby facilitating the reduction of the production cost of enoki mushrooms.
Description
Technical field
The invention belongs to fungus growing technique field, particularly a kind of substratum that promotes golden mushroom mycelium Fast Growth and preparation method thereof.
Background technology
Needle mushroom (
flammulina velutipes), have another name called plain mushroom, belong to Agaricales Bai Mo section genus flammulina, be in China, to develop a kind of edible mushrooms faster in recent years, its mushroom body is milky white fresh and tender, and stem fine hair is few, and mouthfeel is better.Needle mushroom nutrition is extremely abundant, contains protein 8.9% in its dry product, carbohydrate 60.2%, and robust fibre reaches 7.4%, and is rich in the multiple mineral elements such as calcium, iron, phosphorus, has the function that promotes children ' s intelligence development, is called as " intelligence development mushroom ".In addition, the flammulina velutipes that Ion Absorption of Flammulina Velutipes contains, has the functions such as prevention liver and digestive tract diseases generation.Therefore, needle mushroom enjoys people's favor.
Genus flammulina is in macro fungi, and its growth and breeding needs the nutritive substances such as suitable carbon source, nitrogenous source, mineral substance.It is main raw material that existing culture medium for golden mushroom is selected cotton seed hulls (skin), corn cob (powder), wheat bran, soya-bean cake, sawdust, rice straw, rice bran etc. conventionally.But needle mushroom is poor growth in this class substratum, and the production cycle is long.And the production cycle of needle mushroom is the internal factor that affects its production cost.According to investigations, as the production cycle shortens 2～4 days, cost will decline 10～15%.Therefore, how to shorten the production cycle, reducing production costs becomes needle mushroom enterprise problem urgently.
Summary of the invention
The object of this invention is to provide a kind of preparation method who promotes golden mushroom mycelium Fast Growth, shortens the substratum of production cycle.
Technical solution of the present invention comprises the following steps:
1) preparation putrescine solution: putrescine is mixed with distilled water, and obtaining concentration is the putrescine aqueous solution of 10mg/ml;
2) wheat bran, cotton seed hulls, rice bran, the putrescine aqueous solution and water are stirred, obtain substratum compound; In described substratum compound, the mass percent of putrescine is 0.01～0.05%, and the mass percent of water is 60～62%;
3) substratum compound is distributed in culture bottle, sealing is placed on sterilising treatment under 100 ± 5 ℃ of ambient temperature conditions and, after 1.5～2.0 hours, naturally cools to room temperature, is promoted the substratum of golden mushroom mycelium Fast Growth.
Because the present invention has added putrescine composition, the substratum of making has following advantage: 1., guaranteeing, on the basis of the essential nutritive ingredient of needle mushroom growth and breeding, to have simplified substratum, reduced raw materials cost; 2. the growth and breeding that the wheat bran in this substratum, rice bran are needle mushroom provides necessary nutritive substance, cotton seed hulls provides suitable growing environment, putrescine has strengthened the division growth of cell, their collaborative fast-germination, growths that has promoted golden mushroom mycelium, the full bottle of mycelium was down to 22～24 days from 25～28 days, has shortened the production cycle, be beneficial to the production cost that reduces needle mushroom, for people's many a selections of vegetable basket, it is the how a benefit of mushroom agriculture.
In addition, in substratum compound, the mass percent of wheat bran is 15～20%, and the mass percent of cotton seed hulls is 15～20%, and the mass percent of rice bran is 50～60%.
The multivitamins such as the common about cellulose 10% of wheat bran, protein 14%, starch 53%, mineral substance 6% and vitamins B (Lin Lin. the nutritive ingredient of Testa Tritici and exploitation thereof. agricultural science and technology and equipment .2010, (189): 41-42); Rice bran approximately containing 88% dry-matter, 10% Mierocrystalline cellulose, 14% protein, 6% mineral substance and multiple amino acids (Liu Jing, Zhang Shirui. the nutritive value of rice bran and exploitation thereof. Hunan feed .2010, (3): 12-14,17); Cotton seed hulls is approximately containing 4% crude protein, 1.4% crude fat, 44.9% robust fibre and abundant calcium and phosphorus (Feng Weilin, enter the efforts of everyone, Cai Weimin, Fan Lijun, Shen Yingyue, Tian Fangfang. important nutritive ingredient and the Analysis of Heavy Metals of cotton seed hulls, corn cob and wheat bran. edible medicinal fungus, 2012, (4): 220-221).
Adopt the substratum compound of above proportioning can meet the nutritional need that needle mushroom saprophytic fungus is grown.
During the preparation putrescine aqueous solution, first putrescine is dissolved in part distilled water, and then adds another part distilled water, the putrescine aqueous solution that formation concentration is 10mg/ml.
Putrescine is a kind of of biogenic amine, by ornithine decarboxylation, is produced, and is present in each cell, and its content height is directly related with the pH value of cell.Appropriate putrescine has division, promotion tissue growth, the adjusting of enhancing cell growth and the morphogenesis relevant with phytochrome, maintains the effects such as stablizing of cytolemma.In the present invention, by add the putrescine aqueous solution of a small amount of 10mg/ml in substratum compound, not only do not affect trophic component and the culture environment of substratum, and help lend some impetus to division and the mycelial breeding of needle mushroom cell.
Embodiment
Following examples are only used for illustrating the present invention, scope of the present invention are not formed to any restriction.
The wheat bran using in invention, cotton seed hulls, rice bran are purchased from week grain mass wholesale market, Hefei City, Anhui Province; Putrescine is U.S. Sigma product, and purity is greater than 99%; Needle mushroom bacterial classification is commercially available one-level production bacterial strain.
embodiment 1:
1. prepare the putrescine aqueous solution: accurately take 100g putrescine, with a small amount of distilled water, thoroughly dissolve, after dissolving, be settled in 10 liters of containers, obtain the putrescine solution of 10mg/ml, be stored in reagent bottle.
2. prepare burden: take respectively wheat bran 18kg, cotton seed hulls 17kg, rice bran 56kg, measure putrescine aqueous solution 3000ml, add water to 100kg, stir, obtain substratum compound.
After testing, the substratum compound putrescine mass percent being made into is 0.03%.
3. charging: press the charge amount of the every bottled material 680g of 1000ml culture bottle, substratum compound is distributed in culture bottle, after sealing, be placed under 100 ± 5 ℃ of conditions sterilising treatment 1.5 hours.
From sterilising chamber, take out, naturally cool to room temperature, be promoted the substratum of golden mushroom mycelium Fast Growth.
embodiment 2:
One, prepare substratum:
1. prepare the putrescine aqueous solution: accurately take 100g putrescine, with a small amount of distilled water, thoroughly dissolve, after dissolving, be settled in 10 liters of containers, obtain the putrescine solution of 10mg/ml, be stored in reagent bottle.
2. prepare burden: take respectively wheat bran 15 kg, cotton seed hulls 15 kg, rice bran 50kg, measure putrescine aqueous solution 1000ml, add water to 100kg, stir and obtain substratum compound.
After testing, in the substratum compound being made into, water content is 61%, putrescine mass percent is 0.01%.
3. charging: the charge amount of pressing the every bottled material 690g of 1000ml culture bottle, substratum compound is distributed in culture bottle, after sealing, is placed under 100 ± 5 ℃ of conditions sterilising treatment 1.8 hours, naturally cool to room temperature, be promoted the substratum of golden mushroom mycelium Fast Growth.
embodiment 3:
1. prepare the putrescine aqueous solution: accurately take 100g putrescine, with a small amount of distilled water, thoroughly dissolve, after dissolving, be settled in 10 liters of containers, obtain the putrescine solution of 10mg/ml, be stored in reagent bottle.
2. prepare burden: take respectively wheat bran 20 kg, cotton seed hulls 20 kg, rice bran 60 kg, measure putrescine aqueous solution 5000ml, add water and be stirred to 100kg, stir and obtain substratum compound.
After testing, in the substratum compound being made into, water content is 62%, putrescine mass percent is 0.05%.
3. charging: the charge amount of pressing the every bottled material 700g of 1000ml culture bottle, substratum compound is distributed in culture bottle, after sealing, is placed under 100 ± 5 ℃ of conditions sterilising treatment 2.0 hours, naturally cool to room temperature, be promoted the substratum of golden mushroom mycelium Fast Growth.
Two, application:
Needle mushroom first class inoculum is inoculated in respectively in the substratum that above each example makes, and to take daily production substratum be control group, observe respectively mycelial growth, the results are shown in Table 1.
The experimental result of table 1 putrescine to golden mushroom mycelium growth-promoting effect
| Sample source | The mycelium germination time | Mycelial growth situation | Cover with the full bottle time (my god) |
| Embodiment 1 | The 1st day | Mycelia is dense sturdy | 23～24 |
| Embodiment 2 | The 1st day | Mycelia is dense sturdy | 22～24 |
| Embodiment 3 | The 1st day | Mycelia is dense sturdy | 22～23 |
| Control group | The 2nd day | Mycelia is dense sturdy | 25～28 |
From the result of table 1, show that the time advance one day that the substratum of promotion golden mushroom mycelium Fast Growth provided by the invention can make needle mushroom mycelia start growth covers with the more daily production of time of full bottle and shortens 3～4 days with supporting base.
Claims (3)
1. promote a preparation method for the substratum of golden mushroom mycelium Fast Growth, it is characterized in that comprising the following steps:
1) preparation putrescine solution: putrescine is mixed with distilled water, and obtaining concentration is the putrescine aqueous solution of 10mg/ml;
2) wheat bran, cotton seed hulls, rice bran, the putrescine aqueous solution and water are stirred, obtain substratum compound; In described substratum compound, the mass percent of putrescine is 0.01～0.05%, and the mass percent of water is 60～62%;
3) substratum compound is distributed in culture bottle, sealing is placed on sterilising treatment under 100 ± 5 ℃ of ambient temperature conditions and, after 1.5～2.0 hours, naturally cools to room temperature, is promoted the substratum of golden mushroom mycelium Fast Growth.
2. preparation method according to claim 1, the mass percent that it is characterized in that wheat bran in described substratum compound is 15～20%, and the mass percent of cotton seed hulls is 15～20%, and the mass percent of rice bran is 50～60%.
3. according to preparation method described in claim 1 or 2, it is characterized in that first putrescine being dissolved in part distilled water, and then add another part distilled water, the putrescine aqueous solution that formation concentration is 10mg/ml.
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101010433A (en) * | 2004-07-15 | 2007-08-01 | 帝斯曼知识产权资产管理有限公司 | Biochemical synthesis of 1,4-butanediamine |
| CN101684053A (en) * | 2008-09-24 | 2010-03-31 | 上海雪国高榕生物技术有限公司 | Needle mushroom culture medium and preparation method |
| CN101792349A (en) * | 2010-03-22 | 2010-08-04 | 浙江菇老爷食品有限公司 | Preparation method for raw material used for culturing needle mushroom |
| CN102523937A (en) * | 2012-03-12 | 2012-07-04 | 广东星河生物科技股份有限公司 | Culture medium formula for cultivating white beech mushrooms by utilizing needle mushroom fungus chaff and cultivating method thereof |
-
2014
- 2014-04-21 CN CN201410159572.4A patent/CN103951504A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101010433A (en) * | 2004-07-15 | 2007-08-01 | 帝斯曼知识产权资产管理有限公司 | Biochemical synthesis of 1,4-butanediamine |
| CN101684053A (en) * | 2008-09-24 | 2010-03-31 | 上海雪国高榕生物技术有限公司 | Needle mushroom culture medium and preparation method |
| CN101792349A (en) * | 2010-03-22 | 2010-08-04 | 浙江菇老爷食品有限公司 | Preparation method for raw material used for culturing needle mushroom |
| CN102523937A (en) * | 2012-03-12 | 2012-07-04 | 广东星河生物科技股份有限公司 | Culture medium formula for cultivating white beech mushrooms by utilizing needle mushroom fungus chaff and cultivating method thereof |
Non-Patent Citations (1)
| Title |
|---|
| 张勇、谢丽源等: "多胺对离体培养条件下丛枝菌根真菌生长发育的影响", 《菌物系统》 * |
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Application publication date: 20140730 |