CN103947642B - The hay agent of growth hormone wild rice stem progestogenic and spraying method - Google Patents
The hay agent of growth hormone wild rice stem progestogenic and spraying method Download PDFInfo
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- CN103947642B CN103947642B CN201410147462.6A CN201410147462A CN103947642B CN 103947642 B CN103947642 B CN 103947642B CN 201410147462 A CN201410147462 A CN 201410147462A CN 103947642 B CN103947642 B CN 103947642B
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Abstract
The invention discloses the hay agent of growth hormone wild rice stem progestogenic and spraying method, this wild rice stem progestogenic hay agent is composed of the following components: growth hormone, abscisic acid, gibberellin, three's weight ratio is: growth hormone: abscisic acid: gibberellin=2:1-2:1.Spraying method: carry out first time in the wild rice stem growth 4-5 leaf phase and spray, carry out second time with dosage after one week and spray.The present invention is by somatotropin IAA, ABA, GA
3rational proportion, in the suitable time, adopt rational spraying method and the amount of spraying, the pregnant hay of wild rice stem can be promoted, increase Water Bamboo Yield, and IAA, ABA and GA in wild rice stem body
3content does not have significance difference XOR lower than contrast with contrasting, and does not therefore cause wild rice stem hormone in vivo content abnormal.Adopt the hay field of the inventive method management than conventional wild rice stem management and adopt nutrient solution to carry out the hay field managed, its knot hay number and knot hay output significantly improve, and economic benefit significantly improves.
Description
Technical field
The invention belongs to production technology of crops field, be specifically related to a kind ofly promote the pregnant hay of wild rice stem, improve wild rice stem field yield and do not cause the hay agent of growth hormone wild rice stem progestogenic and the spraying method of wild rice stem hormone in vivo content exception.
Background technology
Wild rice stem (
zizania latifoliaturcz.) for grass family wild rice belongs to perennial root herbaceous plant, it is the important aquatic vegetable of one originating in China, extensively cultivate in the Yangtze river basin and areas to the south, become the second largest aquatic vegetable of China except lotus rhizome at present, cultivated area only in Zhejiang Province just up to more than 50 ten thousand mu, has important economic worth.Wild rice stem, also known as wild rice, carving Hu or melon, is one of ancient Chinese five cereals.Wild rice stem is nutritious, and containing more protein, dietary fiber, potassium, phosphorus, sodium, magnesium, nickel, cobalt and thiamine, and moisture is lower, essential amino acids content is enriched, proportion of composing is reasonable, and far exceed other cereals and beans, protein efficiency is higher than flour, rice and soybean.In addition, wild rice stem also has very high medical value, and record according to medical book, wild rice stem taste is sweet cool in nature, has heat-clearing solution to be tired of, promotes the production of body fluid to quench thirst, adjusts the function such as stomach, sharp stool and urine.
The pregnant hay of wild rice stem and its endogenetic fungus-wild rice smut to infect interaction close.There are three kinds of phenotypes when producing in wild rice stem field, i.e. normal wild rice stem (edible), grey hay (wild rice stem stem is full of wild rice smut spore) and male hay (stem does not expand).Histologic section is observed and is shown: wild rice smut is expanded stem at normal wild rice stem and exists in a large number, and the vascular tissue of this external wild rice stem subterranean stem, leaf sheath and bud and parenchyma also observe the distribution of wild rice smut.After wild rice smut infects wild rice stem success, wild rice stem is bloomed and is suppressed, and stimulates wild rice stem stem to expand, forms the edible stem that expands.Having report to think, wild rice stem stem expands is cause due to wild rice stem shoot growth element and the increase of Cytokinin Contents.Research find, when wild rice stem fleshy stem expands, IAA content increasing degree is less, and the duration is longer, before IAA content ratio in fleshy stem enlargement process expands rise, until expand the later stage just occur decline; Fleshy stem cell and expanding of gap thereof may be that IAA plays a major role; GA in wild rice stem enlargement process
3lower with ABA content.Plant hormone IAA, ABA and GA is detected in the wild rice smut zymotic fluid of cultured in vitro
3, wherein IAA content is 0.1306 mgL
-1, ABA content is 0.01367 mgL
-1.As can be seen here, the somatotropin relevant to the pregnant hay of wild rice stem may be produced in Interaction jointly by wild rice stem plant and endogenetic fungus thereof, grows relevant factor very likely have an impact to the pregnant hay of wild rice stem to wild rice stem plant and wild rice smut.
At present, for the pregnant hay of promotion wild rice stem, improve Water Bamboo Yield mainly through methods such as the control of field rich water, breed improvements.There is not yet the similar pregnant hay of promotion wild rice stem, improve the somatotropin series products of Water Bamboo Yield.
Summary of the invention
For the defect existed in prior art, the object of this invention is to provide a kind of rational proportion by different somatotropin and promote the pregnant hay of wild rice stem and output but the hay agent of growth hormone wild rice stem progestogenic and the spraying method that do not cause wild rice stem hormone in vivo content exception.
Described growth hormone wild rice stem progestogenic hay agent, is characterized in that this wild rice stem progestogenic hay agent is composed of the following components: growth hormone, abscisic acid, gibberellin, three's weight ratio is: growth hormone: abscisic acid: gibberellin=2:1-2:1.
Described growth hormone wild rice stem progestogenic hay agent, it is characterized in that this wild rice stem progestogenic hay agent is composed of the following components, growth hormone, abscisic acid, gibberellin, three's weight ratio is: growth hormone: abscisic acid: gibberellin=2:2:1.
Described growth hormone wild rice stem progestogenic hay agent, it is characterized in that this wild rice stem progestogenic hay agent is composed of the following components, growth hormone, abscisic acid, gibberellin, three's weight ratio is: growth hormone: abscisic acid: gibberellin=2:1:1.
The spraying method of described growth hormone wild rice stem progestogenic hay agent, is characterized in that the method is made up of following steps:
1) Jiao Dun selects: select hay Tanaka normally to tie hay and the similar Jiao Dun of proterties in field planting in the last ten-days period in March, conventional field management;
2) preparation of progestogenic hay dilution agent solution: the somatotropin taking formula ratio, dissolve with ethanol, it is 5% that ultra-pure water is settled to mother liquid concentration, and during use, mother liquor dilute with water 1000 times obtains progestogenic hay dilution agent solution;
3) spray: in the 4-5 leaf phase of wild rice stem growth, spray on the blade and leaf sheath of wild rice stem plant with progestogenic hay dilution agent solution, sprayed once every 7 days, spray twice continuously.
The spraying method of described growth hormone wild rice stem progestogenic hay agent, is characterized in that step 2) in concentration of alcohol be 95%.
The spraying method of described growth hormone wild rice stem progestogenic hay agent, is characterized in that step 2) the w/v g:ml of the agent of progestogenic hay and ethanol is 4-5:2.
The spraying method of described growth hormone wild rice stem progestogenic hay agent, is characterized in that in step 3), and the amount of spraying is that every pier wild rice stem sprays 4L progestogenic hay dilution agent solution at every turn.
The present invention compared with prior art its advantage is: by different somatotropin IAA, ABA, GA
3rational proportion, in the suitable time, after adopting rational spraying method and the amount of spraying, the pregnant hay of wild rice stem can be promoted, increase Water Bamboo Yield, adopt the hay field of the inventive method management than the management of conventional wild rice stem and adopt nutrient solution to carry out the hay field managed, its knot hay number and knot hay output significantly improve, and IAA, ABA and GA in wild rice stem body
3content does not have significance difference XOR lower than contrast with contrasting, and therefore do not cause wild rice stem hormone in vivo content abnormal, its economic benefit significantly improves.
Embodiment
The present invention is further illustrated below in conjunction with specific embodiment.
1 field trial
1.1 test materials: test and carried out in experiment field, Dong family's wild rice stem base, Tongxiang City, Zhejiang Province in 2013, test material is No. two, imperial hay, select hay Tanaka in 2012 normally to tie hay and the similar Jiao Dun of proterties, be colonizated in plastic basin (80 × 60 × 50 cm March 25
3) in, every basin 1 pier Basic Seedling.Conventional field management.
1.2 experimental scheme: field trial establishes four treatment combinations altogether, are respectively process one, process two, process three, process four, each process 3 repetition.
Process one formula: growth hormone: abscisic acid: gibberellin=2:2:1; Specifically be formulated as: take 1gIAA, 1gABA and 0.5g GA respectively in proportion
3, be dissolved in 1ml 95 ℅ ethanol, add ultra-pure water constant volume and obtain mother liquor to 50ml, 4 DEG C keep in Dark Place for subsequent use.When spraying, mother liquor is diluted 1000 times of uses.
Process two formula: growth hormone: abscisic acid: gibberellin=2:1:1; Specifically be formulated as: take 1gIAA, 0.5gABA and 0.5g GA respectively in proportion
3, be dissolved in 1ml 95 ℅ ethanol, add ultra-pure water constant volume and obtain mother liquor to 40ml, 4 DEG C keep in Dark Place for subsequent use.When spraying, mother liquor is diluted 1000 times of uses.
Process three formula: process three be nutrient solution prescription: ' trace element water-soluble fertilizer ' dilute with water 500 times, be specially trace element water-soluble fertilizer 50g and add water 2.5kg, ' spray health ' 25g is dissolved in 15kg water, use after equal-volume mixing.Wherein ' trace element water-soluble fertilizer ' is provided by Guilin flower letter plant tissue culture seedling Co., Ltd; ' spray health ' is provided by Shanxi Kaisheng Fertilizer Industry Co., Ltd..
Process four is contrast CK, adopts clear water to spray.
Each process respectively sprays alignment processing formula, and the amount of spraying is that every pier sprays 4L at every turn, all sprays twice, and carry out first time in the 4-5 leaf phase of wild rice stem growth and spray, it is on April 3rd, 2013 that first time sprays the concrete time, and spraying the time is for the second time on April 10th, 2013.At the different times of wild rice stem growth, field statistical observation is carried out to it.
1.3 sample collections: every getting a sample 1 week after first time sprays, Jiao Dun is dug out from field plastic basin during sampling, plant height is measured after cleaning desilt, choose wild rice stem basal part of stem material, each process 3 repetition, after pack with clean husky cloth, mark respectively, liquid nitrogen flash freezer preservation, taking back laboratory, to put-70 DEG C of refrigerators for subsequent use.
1.4 hormone-contents measure: sample 80% methyl alcohol homogenate is extracted, and measures heteroauxin (IAA), gibberellin (GA with Dot-ELISA (ELISA)
3) and the content of abscisic acid (ABA), 3 repetitions established by each sample.
ELISA kit is purchased from Yong Hui bio tech ltd, Beijing.
Hormone extracting method: take 1g wild rice stem stem material, (80% methyl alcohol includes 1.0 mmolL to add 2mL sample extracting solution
-1toluene di-tert-butyl phenol), homogenate is ground to form in ice bath, proceed to 10mL test tube, with the gradation of 2mL extract, mortar is rinsed well again, proceed in test tube in the lump, shake up to be placed in 4 DEG C of refrigerators and leave standstill after 4h, with the centrifugal 15min of 1000 × g, get supernatant and cross C-18 solid-phase extraction column, then the sample after crossing post is proceeded in 10mL plastic centrifuge tube, for subsequent use at being stored in-20 DEG C after nitrogen dries up.
1.5 field knot hay statistics: based on wild rice stem field growing and harvesting standard, respectively the wild rice stem of 4 treatment combinations is gathered on May 24th, 2013 to June 8.The collecting time first of process one, process two, this three process of process three is May 27, and process four first collecting time is May 27.Respectively knot hay number, output, plant height, shell hay weight, hay meat length, hay meat width are measured and added up when gathering.
2. interpretation of result
2.1 wild rice stem change of heights
Somatotropin is used the wild rice stem plant height of rear 4 different times to 4 treatment combinations and is measured and statistical analysis, the results are shown in Table 1.The statistics of table 1 shows: somatotropin treatment combination has obviously facilitation to wild rice stem plant strain growth, the wild rice stem plant of somatotropin treatment combination is significantly higher than other combinations before pregnant hay, but when picking time and between other treatment combinations, there is no significant difference, the results are shown in Table 5.
Table 1: wild rice stem plant height field statistics
2.2 wild rice stem growth hormone changes of contents
With Dot-ELISA, the comparative analysis of basal part of stem IAA changes of contents before the pregnant hay of wild rice stem is shown: the use of somatotropin is little on the impact of Various Zizania latifolia IAA in period content, the results are shown in Table 2.
Table 2: different disposal combination IAA changes of contents before and after process
2.3 wild rice stem GA content changes
With Dot-ELISA to basal part of stem GA before the pregnant hay of wild rice stem
3the comparative analysis of changes of contents shows: somatotropin uses Various Zizania latifolia GA in period
3changes of contents impact seldom.No significant difference between respectively combining when the 7th day, the 14th day, the 21st day after using for the first time; Different disposal combination GA when the 28th day
3content manifests difference, the GA of adjoining tree
3content is significantly higher than the plant of nutrient solution process.The results are shown in Table 3.
Table 3: different disposal combination GA before and after process
3changes of contents
2.4 wild rice stem ABA content changes
With Dot-ELISA, the comparative analysis of basal part of stem ABA changes of contents before the pregnant hay of wild rice stem is shown: spraying basal part of stem ABA content impact before the pregnant hay of wild rice stem of somatotropin is very remarkable.In most of period, the ABA content of adjoining tree is significantly higher than other treatment combinations.The results are shown in Table 4.
Table 4: different disposal combination ABA changes of contents before and after process
2.5 field wild rice stem knot hay analyses
Different disposal combination wild rice stem ties the statistic analysis result of hay in table 5, as shown in Table 5: the use of somatotropin can significantly improve the output of wild rice stem.Process one, process two are relative to control group, and knot hay number improves 20.7,10.0 times respectively, and gross yield improves 25.1,12.6 times respectively.The shell hay representation work of control group combines lower than other, and the hay meat width between different disposal combination does not have notable difference.Hay meat length does not have significant difference between the somatotropin and contrast of different formulations.Compare control group, two formula collecting times of the present invention are late three days.
Table 5: the pregnant hay situation of wild rice stem after process
Claims (7)
1. growth hormone wild rice stem progestogenic hay agent, is characterized in that this wild rice stem progestogenic hay agent is composed of the following components: growth hormone, abscisic acid, gibberellin, three's weight ratio is: growth hormone: abscisic acid: gibberellin=2:1-2:1.
2. growth hormone wild rice stem progestogenic hay according to claim 1 agent, it is characterized in that this wild rice stem progestogenic hay agent is composed of the following components, growth hormone, abscisic acid, gibberellin, three's weight ratio is: growth hormone: abscisic acid: gibberellin=2:2:1.
3. growth hormone wild rice stem progestogenic hay according to claim 1 agent, it is characterized in that this wild rice stem progestogenic hay agent is composed of the following components, growth hormone, abscisic acid, gibberellin, three's weight ratio is: growth hormone: abscisic acid: gibberellin=2:1:1.
4. a spraying method for growth hormone wild rice stem progestogenic hay according to claim 1 agent, is characterized in that the method is made up of following steps:
1) Jiao Dun selects: select hay Tanaka normally to tie hay and the similar Jiao Dun of proterties in field planting in the last ten-days period in March, conventional field management;
2) preparation of progestogenic hay dilution agent solution: the somatotropin taking formula ratio, dissolve with ethanol, it is 5% that ultra-pure water is settled to mother liquid concentration, and during use, mother liquor dilute with water 1000 times obtains progestogenic hay dilution agent solution;
3) spray: in the 4-5 leaf phase of wild rice stem growth, spray on the blade and leaf sheath of wild rice stem plant with progestogenic hay dilution agent solution, sprayed once every 7 days, spray twice continuously.
5. the spraying method of growth hormone wild rice stem progestogenic hay according to claim 4 agent, is characterized in that step 2) in concentration of alcohol be 95%.
6. the spraying method of growth hormone wild rice stem progestogenic hay according to claim 4 agent, is characterized in that step 2) the w/v g:ml of the agent of progestogenic hay and ethanol is 4-5:2.
7. the spraying method of growth hormone wild rice stem progestogenic hay according to claim 4 agent, is characterized in that in step 3), and the amount of spraying is that every pier wild rice stem sprays 4L progestogenic hay dilution agent solution at every turn.
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| CN104585194B (en) * | 2014-12-31 | 2016-04-06 | 安徽农业大学 | A kind of method of wild rice stem progestogenic hay agent and the raising pregnant hay rate of wild rice stem and quality |
| CN107306683A (en) * | 2017-06-28 | 2017-11-03 | 中国计量大学 | A kind of method for postponing wild rice stem knot hay |
| CN107455111A (en) * | 2017-07-10 | 2017-12-12 | 中国计量大学 | A kind of method for promoting wild rice stem to concentrate harvesting |
| CN107278570A (en) * | 2017-07-10 | 2017-10-24 | 中国计量大学 | A kind of method for promoting wild rice stem to tie hay in advance and increase production |
| CN108887268B (en) * | 2018-06-05 | 2021-02-09 | 中国计量大学 | Growth regulator for advancing pregnancy of zizania latifolia and spraying method thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2009079508A1 (en) * | 2007-12-18 | 2009-06-25 | Crop Microclimate Management, Inc. | Methods and compositions for reducing stress in plants |
| CN103314734A (en) * | 2013-05-20 | 2013-09-25 | 安徽宏恩生态农业有限责任公司 | High-quality and high-production cultivating technology of cane shoots |
-
2014
- 2014-04-14 CN CN201410147462.6A patent/CN103947642B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2009079508A1 (en) * | 2007-12-18 | 2009-06-25 | Crop Microclimate Management, Inc. | Methods and compositions for reducing stress in plants |
| CN103314734A (en) * | 2013-05-20 | 2013-09-25 | 安徽宏恩生态农业有限责任公司 | High-quality and high-production cultivating technology of cane shoots |
Non-Patent Citations (2)
| Title |
|---|
| 茭白生育过程中地上各部位内源激素的含量变化;江解增 等;《武汉植物学研究》;20041231;第22卷(第3期);第245-250页 * |
| 茭白的正常茭及其雄茭的主要差异研究;江解增 等;《江苏农学院学报》;19981231;第19卷(第2期);第77-80页 * |
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