CN103947534A - Plane culturing device and method for rice roots - Google Patents
Plane culturing device and method for rice roots Download PDFInfo
- Publication number
- CN103947534A CN103947534A CN201410186579.5A CN201410186579A CN103947534A CN 103947534 A CN103947534 A CN 103947534A CN 201410186579 A CN201410186579 A CN 201410186579A CN 103947534 A CN103947534 A CN 103947534A
- Authority
- CN
- China
- Prior art keywords
- supporting plate
- culture dish
- angle
- base
- seed
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/20—Reduction of greenhouse gas [GHG] emissions in agriculture, e.g. CO2
- Y02P60/21—Dinitrogen oxide [N2O], e.g. using aquaponics, hydroponics or efficiency measures
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention relates to the technical field of experimental material culture, particularly to a plane culturing device and method for rice roots. The device comprises a base, a plurality of supporting plates arranged on the base, linkage rods arranged between the supporting plates, and supporting rods for controlling the angle of the inclination of the supporting plates, wherein a limiting groove which is matched with the connection of the supporting rods for controlling the angle of the inclination of the supporting plates is formed in one side of the base, the base is connected with the supporting plates through rotating shafts, and the supporting plates are connected with the linkage rods through fixed shafts. The culturing method comprises the following steps: A, preparing a culture medium; B, preparing seeds; C, adjusting the angle of the inclination of the supporting plates of the culturing device; D, transplanting sprouted seeds; E, culturing roots. In the technical scheme of the invention, the device is used for adjusting a proper culturing angle, so that the roots are cultured in bevels; therefore, the roots grow on the surface of the culture medium, not only the dynamic growth of the roots can be effectively observed at any time, but also the growth of seedlings is not influenced, and convenience is brought for the research of the roots.
Description
Technical field
The present invention relates to test material culture technique field, be a kind of rice root plane culture apparatus specifically and utilize this device to carry out rice root plane cultural method.
Background technology
Root system is the dynamic interface of Plants and Soils, and it not only has the function that absorbs nutrient and moisture, and the growth of root system simultaneously and turnover directly affect the biogeochemical cycle of carbon in terrestrial ecosystem, nitrogen.Although root system occupies very consequence in terrestrial ecosystem, because root growth is in soil, we are difficult to grasp the true upgrowth situation of root system, so the bottleneck of root system research method k-index research and development always.Therefore, the exploration of root system research method is the importance that root system research worker makes great efforts all the time.Existing research root system method is to adopt root system to excavate out together with soil, then soil is cleaned, and takes out root system.This method is difficult to obtain full dose root system, and the Structure and form of root system also easily destroys, and need to spend a large amount of man power and materials, and reliability and the authenticity of root system data are poor.
At present, observe when rice root carrying out aseptic culture, be generally by planting on medium, under root system of plant, prick in medium; In the time of kinetic measurement extent of the root system, cannot utilize scanner to carry out scanning analysis to root system.Even if analyze and also can exert an influence to experimental data from different perspectives, and waste time and energy.
Summary of the invention
The object of the invention is the problem existing for above-mentioned technology, a kind of rice root plane culture apparatus and method are provided, root growth is on the surface of medium, medium is transparent, root growth Structure and form is true, is convenient to take pictures and root system scanning, is also convenient to the dynamic observation of root growth, the method cost is low, flexible and convenient to use.
For achieving the above object, the technical solution used in the present invention is: a kind of rice root plane culture apparatus, comprise base, be located at several piece supporting plate on base, be located at trace between supporting plate and for controlling the support bar at supporting plate angle of inclination, described base is provided with on one side and coordinates support bar to connect for controlling the stopper slot at supporting plate angle of inclination, between described base and supporting plate, be connected by rotating shaft, between described supporting plate and trace, be connected by fixed axis.
Further, described supporting plate is L shape.
Further, described in several piece, between supporting plate, be equidistant setting.
Another object of the present invention is to provide a kind of rice root plane cultural method, comprises the steps:
The preparation of A, medium: the agar that preparation weight ratio is 0.8%, after autoclaving, pour in culture dish, make the thickness of agar medium reach 4～6mm, obtain transparent agar medium;
The preparation of B, seed: rice paddy seed is first peelled off to shell, the H that is 30% by mass concentration
2o
2sterilization 15min, more thoroughly clean up with distilled water, then seed is sowed in the culture dish that contains agar medium;
The adjustment at C, culture apparatus supporting plate angle of inclination: regulate the support bar on culture apparatus, to the angle that is applicable to rice root cultivation, then culture dish is placed on supporting plate;
The transplanting of D, germinating seed: in gnotobasis, be transplanted in the culture dish that agar medium thickness is 4～6mm with the germinating seed that root has been grown to 2～4mm by aseptic nipper, apart from culture dish upper end 3cm, seed base-root is downward, seals;
E, root system are cultivated: culture dish is reapposed on the cultivation supporting plate that mixes up angle, is placed in illumination cultivation device and cultivates; Can readjust or calibrate if needed during this time cultivation support, reach the required requirement of test; In addition, its temperature setting is set to 28 DEG C of daytimes, and at 22 DEG C of nights, light application time is 16h, dark 8h, and intensity of illumination is 100 μ molm
-2s
-1; Incubation time is Root Growth of Rice situation and the test requirements document of arriving according to the observation, choose reasonable.
Further, in described step D, in each culture dish, transplant 2～5 germinating seeds.
Further, culture dish is selected organic transparent plastic ware, and culture dish is made up of lid and bottom, and the thickness of its middle cover is 1cm, and the thickness at the end is 1.5cm, uses before use 10k Gy
60co γ ray sterilization 24h.
Useful technique effect of the present invention: in technical scheme of the present invention, utilize this device to regulate appropriate incubation angle, root system inclined-plane is cultivated, thereby reach root growth in media surface, can not only effectively observe at any time the dynamic growth of root system, and do not affect the growth of seedling, for the research of root system offers convenience.
Brief description of the drawings
In order to be illustrated more clearly in the embodiment of the present invention or technical scheme of the prior art, to the accompanying drawing of required use in embodiment or description of the Prior Art be briefly described below, apparently, accompanying drawing in the following describes is only some embodiments of the present invention, for those of ordinary skill in the art, do not paying under the prerequisite of creative work, can also obtain according to these accompanying drawings other accompanying drawing.
Fig. 1 is the structural representation of culture apparatus of the present invention;
Fig. 2 is the structural representation that apparatus of the present invention are carried out the cultivation of rice root plane;
In figure: 1 base; 2 supporting plates; 3 traces; 4 support bars; 5 rotating shafts; 6 fixed axis; 7 stopper slots.
Embodiment
Below in conjunction with the accompanying drawing in the embodiment of the present invention, the technical scheme in the embodiment of the present invention is clearly and completely described, obviously, described embodiment is only the present invention's part embodiment, instead of whole embodiment.Based on the embodiment in the present invention, those of ordinary skill in the art, not making the every other embodiment obtaining under creative work prerequisite, belong to the scope of protection of the invention.
Embodiment 1
A rice root plane culture apparatus as shown in Figure 1, comprises base 1, is located at 7 supporting plates 2 on base 1, is located at trace 3 between supporting plate 2 and for controlling the support bar 4 at supporting plate 2 angles of inclination; The effect that is provided with trace 3 between supporting plate 2 is the control that facilitates angle of inclination between all supporting plates 2, in the time that being used, reality adjusts separately angle without every supporting plate 2, by the interlink function of support bar 4 and trace 3, realize the unified of angle of inclination of all supporting plates 2 and adjust; Base 1 is provided with on one side and coordinates support bar 4 to connect the stopper slot 7 for controlling supporting plate 2 angles of inclination, in the time that reality is used, can control by support bar 4 angle of inclination of supporting plate 2 in the position of stopper slot 7; Between base 1 and supporting plate 2, be connected by rotating shaft 5, adopt the adjustment of rotating shaft 5 angles easy to connect, between supporting plate 2 and trace 3, be connected by fixed axis 6.
Wherein, supporting plate 2 is L shape, and the supporting plate 2 of L shape can ensure that supporting plate 2 can only be along on one side carrying out angle of inclination adjustment, the culture dish of avoiding operate miss the to occur phenomenon that drops.
Wherein, 7 supporting plates 2 are equidistant setting.
As shown in Figure 2, utilize said apparatus to carry out rice root plane cultural method, comprise the steps:
The preparation of A, medium: the agar that preparation weight ratio is 0.8%, after autoclaving, pour in culture dish, make the thickness of agar medium reach 4～6mm, obtain transparent agar medium;
The preparation of B, seed: rice paddy seed is first peelled off to shell, the H2O2 that is 30% by the mass concentration 15min that sterilizes, more thoroughly clean up with distilled water, then seed is sowed in the culture dish that contains agar medium;
The adjustment at C, culture apparatus supporting plate angle of inclination: regulate the support bar on culture apparatus, to the angle that is applicable to rice root cultivation, then culture dish is placed on supporting plate;
The transplanting of D, germinating seed: in gnotobasis, be transplanted in the culture dish that thickness is 4～6mm with the germinating seed that root has been grown to 2～4mm by aseptic nipper, apart from culture dish upper end 3cm, seed base-root is downward, seal, in each culture dish, transplant 2～5 germinating seeds;
E, root system are cultivated: culture dish is reapposed on the cultivation supporting plate that mixes up angle, is placed in illumination cultivation device and cultivates;
In cultivation, culture dish is selected organic transparent plastic ware, is made up of lid and bottom, and the thickness of its middle cover is 1cm, and the thickness at the end is 1.5cm, uses before use 10k Gy
60co γ ray sterilization 24h.The foregoing is only preferred embodiment of the present invention, in order to limit the present invention, within the spirit and principles in the present invention not all, any amendment of doing, be equal to replacement, improvement etc., all should be included in
Within protection scope of the present invention.
Claims (6)
1. rice root plane culture apparatus, it is characterized in that, comprise base (1), be located at the several piece supporting plate (2) on base (1), be located at trace (3) between supporting plate (2) and for controlling the support bar (4) at supporting plate (2) angle of inclination, described base (1) one side is provided with and coordinates support bar (4) to connect the stopper slot (7) that is used for controlling supporting plate (2) angle of inclination, between described base (1) and supporting plate (2), be connected by rotating shaft (5), between described supporting plate (2) and trace (3), be connected by fixed axis (6).
2. rice root plane culture apparatus according to claim 1, is characterized in that, described supporting plate (2) is L shape.
3. rice root plane culture apparatus according to claim 2, is characterized in that, between supporting plate (2), is equidistant setting described in several piece.
4. rice root plane cultural method, is characterized in that, comprises the steps:
The preparation of A, medium: the agar that preparation weight ratio is 0.8%, after autoclaving, pour in culture dish, make the thickness of agar medium reach 4～6mm, obtain transparent agar medium;
The preparation of B, seed: rice paddy seed is first peelled off to shell, the H that is 30% by mass concentration
2o
2sterilization 15min, then clean up with distilled water, then seed is sowed in the culture dish that contains agar medium;
The adjustment at C, culture apparatus supporting plate angle of inclination: regulate the support bar on culture apparatus, to the angle that meets rice root cultivation, then culture dish is placed on supporting plate;
The transplanting of D, germinating seed: in gnotobasis, be transplanted in the culture dish that agar medium thickness is 4～6mm with the germinating seed that root has been grown to 2～4mm by aseptic nipper, apart from culture dish upper end 3cm, seed base-root is downward, seals;
E, root system are cultivated: culture dish is reapposed on the cultivation supporting plate that mixes up angle, is placed in illumination cultivation device and cultivates.
5. rice root plane cultural method according to claim 4, is characterized in that, transplants 2～5 germinating seeds in described step D in each culture dish.
6. rice root plane cultural method according to claim 4, is characterized in that, culture dish is selected organic transparent plastic ware, and culture dish is made up of lid and bottom, and the thickness of its middle cover is 1cm, and the thickness at the end is 1.5cm, uses before use 10k Gy
60co γ ray sterilization 24h.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410186579.5A CN103947534B (en) | 2014-05-05 | 2014-05-05 | Rice root plane culture apparatus and method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410186579.5A CN103947534B (en) | 2014-05-05 | 2014-05-05 | Rice root plane culture apparatus and method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103947534A true CN103947534A (en) | 2014-07-30 |
| CN103947534B CN103947534B (en) | 2016-01-20 |
Family
ID=51325009
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410186579.5A Active CN103947534B (en) | 2014-05-05 | 2014-05-05 | Rice root plane culture apparatus and method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103947534B (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106718777A (en) * | 2016-11-15 | 2017-05-31 | 江西省红壤研究所 | A kind of rice at whole growth periods hydroponics System and method for |
| CN107655888A (en) * | 2017-09-09 | 2018-02-02 | 华中农业大学 | One kind is applied to rice root two dimensional image harvester in root box soil |
Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB444377A (en) * | 1934-09-01 | 1936-03-19 | Hermann Beck | Cabinet for promoting germination of seeds and growth of seedlings |
| GB472873A (en) * | 1936-03-26 | 1937-09-27 | Henry Hilton Blair Lund | Improvements in or relating to apparatus for the germination of seeds and the growth of seedlings |
| JP2010259345A (en) * | 2009-04-30 | 2010-11-18 | Akazawa Sogo Kenkyusho:Kk | Hydroponic apparatus and cultivation bed to be used for the same |
| CN102106252A (en) * | 2009-12-28 | 2011-06-29 | 中国科学院沈阳应用生态研究所 | Display system suitable for comparing and observing growth of plant root systems |
| CN102523977A (en) * | 2011-12-09 | 2012-07-04 | 河南科技大学 | Culture method for rice seedlings |
| CN202617902U (en) * | 2012-05-22 | 2012-12-26 | 中国水稻研究所 | Soilless nutritional board for rice plate seedling raising |
| CN202998993U (en) * | 2012-12-18 | 2013-06-19 | 南京农业大学 | Plant water planting and root system observation device |
| CN103283548A (en) * | 2013-06-17 | 2013-09-11 | 金寨县冠友盆景园艺有限公司 | Rice soilless culture method |
| CN203435434U (en) * | 2013-08-08 | 2014-02-19 | 华中农业大学 | Crop root dynamic observation test device |
| CN203840890U (en) * | 2014-05-05 | 2014-09-24 | 中国水稻研究所 | Rice root system plate culture device |
-
2014
- 2014-05-05 CN CN201410186579.5A patent/CN103947534B/en active Active
Patent Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB444377A (en) * | 1934-09-01 | 1936-03-19 | Hermann Beck | Cabinet for promoting germination of seeds and growth of seedlings |
| GB472873A (en) * | 1936-03-26 | 1937-09-27 | Henry Hilton Blair Lund | Improvements in or relating to apparatus for the germination of seeds and the growth of seedlings |
| JP2010259345A (en) * | 2009-04-30 | 2010-11-18 | Akazawa Sogo Kenkyusho:Kk | Hydroponic apparatus and cultivation bed to be used for the same |
| CN102106252A (en) * | 2009-12-28 | 2011-06-29 | 中国科学院沈阳应用生态研究所 | Display system suitable for comparing and observing growth of plant root systems |
| CN102523977A (en) * | 2011-12-09 | 2012-07-04 | 河南科技大学 | Culture method for rice seedlings |
| CN202617902U (en) * | 2012-05-22 | 2012-12-26 | 中国水稻研究所 | Soilless nutritional board for rice plate seedling raising |
| CN202998993U (en) * | 2012-12-18 | 2013-06-19 | 南京农业大学 | Plant water planting and root system observation device |
| CN103283548A (en) * | 2013-06-17 | 2013-09-11 | 金寨县冠友盆景园艺有限公司 | Rice soilless culture method |
| CN203435434U (en) * | 2013-08-08 | 2014-02-19 | 华中农业大学 | Crop root dynamic observation test device |
| CN203840890U (en) * | 2014-05-05 | 2014-09-24 | 中国水稻研究所 | Rice root system plate culture device |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106718777A (en) * | 2016-11-15 | 2017-05-31 | 江西省红壤研究所 | A kind of rice at whole growth periods hydroponics System and method for |
| CN106718777B (en) * | 2016-11-15 | 2020-06-05 | 江西省红壤研究所 | Soilless planting system and method for rice in whole growth period |
| CN107655888A (en) * | 2017-09-09 | 2018-02-02 | 华中农业大学 | One kind is applied to rice root two dimensional image harvester in root box soil |
Also Published As
| Publication number | Publication date |
|---|---|
| CN103947534B (en) | 2016-01-20 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101658107B (en) | Method for in-situ dynamic observation and measurement of root system of plant | |
| CN101663968B (en) | Plant cultivating method for dynamically observing and measuring roots in situ | |
| CN201142831Y (en) | Integrated box for germination/water culture of small-sized seeds | |
| CN102696463A (en) | Arabidopsis hydroponic method capable of effectively preventing generation of green algae | |
| CN204393035U (en) | The plant that a kind of water planting, sand culture combine is without native culture apparatus | |
| CN207410963U (en) | A kind of forestry seedling equipment for promoting plant health growth | |
| CN103588517A (en) | Efficient sugarcane seedling cultivating matrix | |
| CN103947534B (en) | Rice root plane culture apparatus and method | |
| CN109275395A (en) | The identification of machine transplanting of rice rice shoot burst and method of filling the gaps with seedlings | |
| CN102160503A (en) | A dynamic measurement method of a potted plant root system | |
| CN109005964A (en) | A kind of aseptic filter paper cultural method for researching plant root and environmental interaction | |
| CN202818919U (en) | Soybean seed germination and water culture device for laboratory | |
| CN208387514U (en) | A kind of plant cultivating device for root system of plant two dimension in-situ dynamic observation and measurement | |
| CN204317135U (en) | A kind of vegetable growing device | |
| CN203840890U (en) | Rice root system plate culture device | |
| CN105875143B (en) | A kind of field pipe plants the test method of fertilizer | |
| CN206651111U (en) | A kind of Self-water absorption type vegetables nursery box | |
| CN102326508B (en) | Combined-type water culture device for root division experiment of plants and method by utilizing device to carry out water culture | |
| CN211407170U (en) | Water culture device for arabidopsis thaliana | |
| CN210695373U (en) | Sand water culture device for culturing crop seedlings | |
| CN207733389U (en) | A kind of device of laboratory water planting identification rice root form | |
| CN105103940A (en) | Novel edible fungus earthing and seed production method | |
| CN110463464A (en) | A kind of plant cultivation method based on plant roots in situ dynamic observation and measurement | |
| CN104737669B (en) | A kind of plumelet resistance detection means and plumelet resistance detection method | |
| CN215683586U (en) | Tool for observing cultivated crops in situ |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant |