CN110463464A - A kind of plant cultivation method based on plant roots in situ dynamic observation and measurement - Google Patents
A kind of plant cultivation method based on plant roots in situ dynamic observation and measurement Download PDFInfo
- Publication number
- CN110463464A CN110463464A CN201810436545.5A CN201810436545A CN110463464A CN 110463464 A CN110463464 A CN 110463464A CN 201810436545 A CN201810436545 A CN 201810436545A CN 110463464 A CN110463464 A CN 110463464A
- Authority
- CN
- China
- Prior art keywords
- plant
- transparent
- culture medium
- root system
- cultivation method
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G9/00—Cultivation in receptacles, forcing-frames or greenhouses; Edging for beds, lawn or the like
- A01G9/02—Receptacles, e.g. flower-pots or boxes; Glasses for cultivating flowers
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Botany (AREA)
- Cultivation Of Plants (AREA)
Abstract
The invention discloses a kind of plant cultivation method based on plant roots in situ dynamic observation and measurement, 1) it is the following steps are included: set semi-transparent cultivating container and somatomedin；2) Seed sterilization and vernalization transplant seedlings to cultivating container transparent described in step 1) after budding, root system of plant are kept to grow naturally in the transparent somatomedin, and root system is scanned and observed for three-dimensional laser scanner.Plant cultivation method of the invention, it realizes that the root system of plant is grown naturally in transparent growing environment, by carrying out three-dimensional visualization analysis to plant roots in situ upgrowth situation, can directly reflect the three dimensional growth situation of root system, destructive detection is not needed, and realizes dynamic observation.In addition, by the component for changing nutrient added by culture medium, supply situation of the adjustable somatomedin to root system of plant nutrient.
Description
Technical field
The present invention relates to field of plant growing technology, are based on plant roots in situ dynamic observation and survey more particularly to one kind
Fixed plant cultivation method.
Background technique
Root system is the major organs of plant absorption nutrient and moisture, and the only good root system of growth and development is just able to satisfy plant
The needs of growth.Therefore, researching plant root grows the demand to understanding plant to nutrient and its has ten with the relationship of environment
Divide important meaning.Certain progress is had been achieved with to the research of root system at present, but under natural growthing condition, due to soil
The opacity of medium and the complexity of root structure, it is difficult to carry out home position observation and measurement.The root system shape having been reported so far
State configuration data is the data that destructive sampling obtains mostly, and ex situ data, and root dry mass and nutrient efficiency mostly
Research belongs to qualitative research, and the quantitative study of root dry mass and nutrient efficiency is also rarely reported.In addition, existing research at present
Most of is all the root dry mass data and the research of nutrient efficiency at a certain moment, dynamic original position root dry mass data and nutrient efficiency
Research then have no technical report.The basis for completing the research of dynamic root dry mass data in situ and nutrient efficiency is to realize to plant
Object root system is grown naturally in transparent environment.
Summary of the invention
The object of the present invention is to provide a kind of plant cultivation method based on plant roots in situ dynamic observation and measurement, with
Effectively overcome above-mentioned the deficiencies in the prior art.
The scheme that the present invention solves technical problem is: a kind of to be planted based on the plant of plant roots in situ dynamic observation and measurement
Culture method, it the following steps are included:
1) semi-transparent cultivating container and somatomedin are set；
2) Seed sterilization and vernalization transplant seedlings to cultivating container transparent described in step 1) after budding, keep root system of plant described
It is grown naturally in transparent somatomedin, root system is scanned and observed for three-dimensional laser scanner.
In step 1), the transparent cultivating container is cylindrical plexiglass box, and one is equipped on cultivating container
A openable container cover is equipped with pass through aperture in the middle part of container cover；The somatomedin is transparent culture medium, transparent culture medium
It is the mixture of the corresponding nutrient solution and 0.15%PhytagelTM that are adapted to plant.
A height of 15~the 25cm of the cylinder plexiglass box, diameter be 10~15cm, through bore dia be 15~
25mm。
The transparent culture medium configuration step is as follows:
A) culture medium is stirred evenly, adjusts pH value；Plexiglass box uses ultraviolet disinfection 1 hour, spare；
B) culture medium is sterilized 30 minutes at 121 DEG C；It is poured into after sterilizing in above-mentioned sterilized plexiglass box；
C) culture medium stands cooling, solidification.
The invention has the advantages that: plant cultivation method of the invention realizes the root system of plant in transparent life
It is grown naturally in long environment, by carrying out three-dimensional visualization analysis to plant roots in situ upgrowth situation, can directly reflect root system
Three dimensional growth situation, do not need destructive detection, and realize dynamic observation.In addition, being supported added by culture medium by changing
The component divided, supply situation of the adjustable somatomedin to root system of plant nutrient.Therefore, it can also be grown with the present invention
The research of the supply situation of nutrient and relationship of plant root growth etc. in medium.
Specific embodiment
Below with reference to embodiment, the present invention is described in further detail:
Plant cultivation method of the invention, it the following steps are included:
1) semi-transparent cultivating container and somatomedin are set；
2) Seed sterilization and vernalization transplant seedlings to cultivating container transparent described in step 1) after budding, keep root system of plant described
It grows in transparent somatomedin, root system is scanned naturally using three-dimensional laser scanner, carry out root system 3-d modelling
Home position observation and image capture.
In step 1), the transparent cultivating container is cylindrical plexiglass box, and one is equipped on cultivating container
A openable container cover is equipped with pass through aperture in the middle part of container cover；The somatomedin is transparent culture medium, transparent culture medium
It is the mixture of the corresponding nutrient solution and 0.15%PhytagelTM that are adapted to plant.
It a height of 20cm of the cylinder plexiglass box, diameter cm, through bore dia is 20mm.
The transparent culture medium configuration step is as follows:
A) culture medium is stirred evenly, adjusting pH value is 5.8；Plexiglass box uses ultraviolet disinfection 1 hour, spare；
B) culture medium is sterilized 30 minutes at 121 DEG C；It is poured into after sterilizing in above-mentioned sterilized plexiglass box；
C) culture medium stand cooling, solidification, when culture medium solidifies fastly, container cannot be moved, with prevent culture medium solidify after in
Face generates bubble.
Plant cultivation method of the invention realizes that the root system of plant is grown naturally in transparent growing environment, by right
Plant roots in situ upgrowth situation carries out three-dimensional visualization analysis, can directly reflect the three dimensional growth situation of root system, not need brokenly
The detection of bad property, and realize dynamic observation.In addition, adjustable growth is situated between by the component for changing nutrient added by culture medium
The supply situation of confrontation root system of plant nutrient.
Claims (4)
1. a kind of plant cultivation method based on plant roots in situ dynamic observation and measurement, it is characterised in that: it includes following
Step:
1) semi-transparent cultivating container and somatomedin are set；
2) Seed sterilization and vernalization transplant seedlings to cultivating container transparent described in step 1) after budding, keep root system of plant described
It is grown naturally in transparent somatomedin, root system is scanned and observed for three-dimensional laser scanner.
2. a kind of plant cultivation method based on plant roots in situ dynamic observation and measurement according to claim 1,
Be characterized in that: in step 1), the transparent cultivating container is cylindrical plexiglass box, and one is equipped on cultivating container
A openable container cover is equipped with pass through aperture in the middle part of container cover；The somatomedin is transparent culture medium, transparent culture medium
It is the mixture of the corresponding nutrient solution and 0.15%PhytagelTM that are adapted to plant.
3. a kind of plant cultivation method based on plant roots in situ dynamic observation and measurement according to claim 2,
It being characterized in that: a height of 15~25cm of the cylinder plexiglass box, diameter be 10~15cm, through bore dia be 15~
25mm。
4. a kind of plant cultivation method based on plant roots in situ dynamic observation and measurement according to claim 2,
Be characterized in that: the transparent culture medium configuration step is as follows:
A) culture medium is stirred evenly, adjusts pH value；Plexiglass box uses ultraviolet disinfection 1 hour, spare；
B) culture medium is sterilized 30 minutes at 121 DEG C；It is poured into after sterilizing in above-mentioned sterilized plexiglass box；
C) culture medium stands cooling, solidification.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810436545.5A CN110463464A (en) | 2018-05-09 | 2018-05-09 | A kind of plant cultivation method based on plant roots in situ dynamic observation and measurement |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810436545.5A CN110463464A (en) | 2018-05-09 | 2018-05-09 | A kind of plant cultivation method based on plant roots in situ dynamic observation and measurement |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN110463464A true CN110463464A (en) | 2019-11-19 |
Family
ID=68503370
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810436545.5A Pending CN110463464A (en) | 2018-05-09 | 2018-05-09 | A kind of plant cultivation method based on plant roots in situ dynamic observation and measurement |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN110463464A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115152477A (en) * | 2022-08-24 | 2022-10-11 | 江西省农业科学院植物保护研究所 | Device and method for observing underground part of soil-cultured plant |
-
2018
- 2018-05-09 CN CN201810436545.5A patent/CN110463464A/en active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115152477A (en) * | 2022-08-24 | 2022-10-11 | 江西省农业科学院植物保护研究所 | Device and method for observing underground part of soil-cultured plant |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101658107B (en) | Method for in-situ dynamic observation and measurement of root system of plant | |
| CN101663968B (en) | Plant cultivating method for dynamically observing and measuring roots in situ | |
| CN102150624B (en) | Tissue culture and rapid propagation method for pinellia tuber plant | |
| CN102246695A (en) | Method for preparing common bletilla pseudobulb in culture vessel and special culture media thereof | |
| CN101263787A (en) | Louis iris tissue culture fast seedling establishment and ecological application method | |
| CN102792893A (en) | Tissue culture propagating method of Rhododendron agastum | |
| CN107896986A (en) | A kind of high quality bog moss rapid propagation method | |
| CN204104458U (en) | A kind of device observing rice seedling Root morphology | |
| CN204393035U (en) | The plant that a kind of water planting, sand culture combine is without native culture apparatus | |
| CN110463464A (en) | A kind of plant cultivation method based on plant roots in situ dynamic observation and measurement | |
| CN109005964A (en) | A kind of aseptic filter paper cultural method for researching plant root and environmental interaction | |
| CN105028192A (en) | Culture medium series for rapidly breeding dendrobium nobile seedlings and tissue culture method | |
| CN103283602B (en) | Culture method of embryoid by inducing asparagus microspore | |
| CN105454044A (en) | Tissue culture method for Punica granatum and culture medium | |
| CN101658133A (en) | Method for quickly and directly establishing albizzia regeneration plant system | |
| CN208387514U (en) | A kind of plant cultivating device for root system of plant two dimension in-situ dynamic observation and measurement | |
| CN104920219B (en) | Dendrobium devonianum rapid propagation seedling survival culture medium series and tissue culture method | |
| CN203435434U (en) | Crop root dynamic observation test device | |
| CN101328608B (en) | Growth method of mercuric bromide single crystal | |
| CN102511397A (en) | Method for inducing populus calli and induction culture medium | |
| CN105557527A (en) | Manufacturing method for artificial cymbidium seeds | |
| CN103947534A (en) | Plane culturing device and method for rice roots | |
| JP2011036154A (en) | Method for producing moss, and device for producing moss | |
| CN106171991B (en) | A kind of expanding propagation method of fructus schisandrae | |
| CN203968763U (en) | The observation experiment bottle of a kind of growth hormone on growth and development of plants impact |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20191119 |
|
| WD01 | Invention patent application deemed withdrawn after publication |