CN103933410A

CN103933410A - Medicinal use and preparation method of traditional Chinese medicine composition

Info

Publication number: CN103933410A
Application number: CN201310021647.8A
Authority: CN
Inventors: 赵志全
Original assignee: Lunan Pharmaceutical Group Corp
Current assignee: Lunan Pharmaceutical Group Corp
Priority date: 2013-01-21
Filing date: 2013-01-21
Publication date: 2014-07-23

Abstract

The invention discloses use of a traditional Chinese medicine composition in preparation of a drug for treatment of hemorrhoids, and belongs to the field of medicines. The traditional Chinese medicine composition comprises many traditional Chinese medicines such as astragalus, honeysuckle, golden cypress, atractylodes rhizome, coix seed, radix scrophulariae, Chinese angelica, radix paeoniae alba, radix glycyrrhizae, leech, whole scorpion and centipede. Researches on various hemorrhoid models show that the traditional Chinese medicine composition has remarkable pharmacodynamics effects of inflammatory resistance and analgesia and effects of activating blood circulation to dissipate stasis when in use of treatment of hemorrhoids, and has good therapeutic effects on various animal models of hemorrhoids. The traditional Chinese medicine composition has a comprehensive effect on treatment of hemorrhoids, is high in drug application compliance, has a better therapeutic effect that drugs for treatment of hemorrhoids in the prior art, and has broad market prospects.

Description

Medical usage of a kind of Chinese medicine composition and preparation method thereof

Technical field

The present invention relates to a kind of new purposes of Chinese medicine composition, be specifically related to the purposes of Chinese medicine composition of the present invention in preparing medicine for treaching hemorrhoie, belong to field of medicaments.

Background technology

Hemorrhoid are a kind of common anal and intestinal diseases clinically, comprise internal hemorrhoid, external hemorrhoid and mixed hemorrhoid, and any age all can fall ill, but with age growth, sickness rate increases.Clinical symptoms main manifestations is hemorrhage, pain, infection, falling inflation, pruritus etc., and 90% left and right is internal hemorrhoid, and internal hemorrhoid be take again hemorrhage and deviate from type as main.Hemorrhoid are caused prolonged and repeatedly to be gone out blood solution and easily causes anemia and infection etc.Modern medicine thinks that the generation of hemorrhoid is mainly because hemorrhoidal plexus blood backflow is limited, the local lump that hemorrhoidal plexus vasodilation is tortuous or anus pad pathologic hypertrophy is shifted and prolapses and form, with congestion, hamartoplasia and structural deterioration etc., but the true cause of its morbidity it be unclear that.The object of clinical drug therapy is mainly the symptom of alleviating or eliminate hemorrhoid, as hemorrhage, pain, edema, deviate from, pruritus etc., and cannot make the hemorrhoid body or the hemorrhoidal tissue that have produced pathological changes return to normal condition.

Traditional theory of Chinese medical science is thought: hemorrhoid are damp invasion of lower energizer, spleen-QI sinking, caused by energy stagnation and blood stasis, is mainly the weakness due to wall of vein, has lost normal elasticity, hold concurrently because of eating and drinking without temperance, production of dryness-heat in the interior, tenesmus large intestine and sitting, heavy burden, long journey etc., cause hematogenous blockage, blood stagnation, ties stagnant not loose forming.The symptoms such as clinical manifestation is local inflammation, swell and ache, have blood in stool, deviate from, its cause of disease is because QI and blood is sunk, the poly-anus of knot, dyspepsia is loose, affects anus lymphatic return and causes.The hot dryness accumulated in the stomach and intestine of intestinal can cause anal fissure, take clinically and has blood in stool, deviates from, swells and ache as main manifestations; Hemorrhage is due to blood stagnation, and hematogenous blockage causes vein blood vessel to break.Treatment should be take heat clearing and damp drying, blood circulation promoting and blood stasis dispelling, reducing swelling and alleviating pain as main.

Chinese medicine composition composition granule of the present invention is three class Chinese patent medicines for the treatment of thrombophlebitis, its prescription and preparation method have obtained Chinese invention patent (ZL03100187.4), the effect with heat-clearing and toxic substances removing, disperse blood stasis and dredge collateral, damp eliminating detumescence, for the superficial thrombophlebitis due to dampness and heat stasis venation, lower limb swelling, pain, the colour of skin due to non-acute stage venous thrombosis are dark red or with streak thing.Prescription is comprised of taste Chinese medicines such as the Radix Astragali, Flos Lonicerae, Cortex Phellodendri, Rhizoma Atractylodis, Semen Coicis, Radix Scrophulariae, Radix Angelicae Sinensis, the Radix Paeoniae Alba, Radix Glycyrrhizae, Hirudo, Scolopendra, Scorpios 12, merged the Simiao San with clearing heat and expelling damp function, the SIMIAOYONGAN TANG with heat-clearing and toxic substances removing, promoting blood circulation and stopping pain function, having removing toxic substances search pick, the Zhijing San of relieving convulsion eliminating stagnation effect, the recipe that there is the peony and licorice decoction of emergency solution contraction, relieving pain by regulating nutrient QI effect and there is the treatment thrombotic disease such as Radix Angelicae Sinensis decoction for tonifying blood of QI invigorating and blood producing function.The advantage that it has followed all sides, has made up former side's deficiency, brings out the best in each other, and left and right is taken into account, and clinical trial shows that this Chinese medicine composition is effective prescription for the treatment of thrombophlebitis.

CN101618161B discloses a kind of Chinese medicine composition (hereinafter to be referred as " superfine powder venation Shutong granules ") of thrombophlebitis and preparation method of this Chinese medicine composition for the treatment of.This Chinese medicine composition Hirudo, Scolopendra and Scorpio are processed through micronizing, can improve effective ingredient in gastrointestinal dissolubility and bioavailability, and accelerate the drug effect time, the therapeutic effect of thrombophlebitis is better than to the product of Ordinary pulverization processes.< < treatment by Chinese herbs hemorrhoid general introduction > > [Wei Jingjing, Yang Xiuhui. treatment by Chinese herbs hemorrhoid general introduction [J]. practical Chinese medicine magazine .2007 (05)] summarized present situation and the administering mode of existing treatment by Chinese herbs hemorrhoid, it thinks that mostly hemorrhoid are because damp-heat accumulation, stagnation of QI-blood are due to the anorectum.The non-operative treatment such as oral medicinal herb or external, has avoided the appearance of complication, is specially adapted to the patients such as old age, body void, children's, and Chinese medicine has unique advantage aspect treatment hemorrhoid.

Summary of the invention

The object of the invention is to provide a kind of Chinese medicine composition for the treatment of hemorrhoid, this Chinese medicine composition is by multi-flavor ingredients such as the Radix Astragali, Flos Lonicerae, Cortex Phellodendri, Rhizoma Atractylodis, Semen Coicis, Radix Scrophulariae, Radix Angelicae Sinensis, the Radix Paeoniae Alba, Radix Glycyrrhizae, Hirudo, Scorpio, Scolopendras, it has the effect of significant antiinflammatory, analgesia pharmacodynamic action and activating blood circulation to dissipate blood stasis during for hemorrhoid treating, therapeutic effect is excellent, has wide market prospect.

The invention discloses the purposes of a kind of Chinese medicine composition in preparing medicine for treaching hemorrhoie, described Chinese medicine composition contains following Chinese medicinal components:

Radix Astragali 300-700 weight portion Flos Lonicerae 300-700 weight portion Cortex Phellodendri 100-400 weight portion

Rhizoma Atractylodis 100-400 weight portion Radix Scrophulariae 300-700 weight portion Radix Angelicae Sinensis 100-400 weight portion

Radix Paeoniae Alba 100-400 weight portion Semen Coicis 300-700 weight portion.

Preferably, in above-mentioned Chinese medicine composition, also contain following Chinese medicinal components:

Scolopendra 10-40 weight portion Scorpio 50-100 weight portion Hirudo 180-280 weight portion Radix Glycyrrhizae 50-100 weight portion.

The preferred weight proportioning of described Chinese medicine composition raw material of Chinese medicine is:

Radix Astragali 400-550 weight portion Flos Lonicerae 450-550 weight portion Cortex Phellodendri 200-300 weight portion

Rhizoma Atractylodis 200-300 weight portion Radix Scrophulariae 450-550 weight portion Radix Angelicae Sinensis 200-300 weight portion

Radix Paeoniae Alba 200-280 weight portion Semen Coicis 450-550 weight portion Scolopendra 10-40 weight portion

Scorpio 50-100 weight portion Hirudo 180-280 weight portion Radix Glycyrrhizae 50-100 weight portion.

The optimum weight proportion of described Chinese medicine composition raw material of Chinese medicine is:

The Radix Astragali 500 weight portion Flos Lonicerae 500 weight portion Cortex Phellodendri 250 weight portions

Rhizoma Atractylodis 250 weight portion Radix Scrophulariae 500 weight portion Radix Angelicae Sinensis 250 weight portions

The Radix Paeoniae Alba 250 weight portion Semen Coicis 500 weight portion Scolopendra 20 weight portions

Scorpio 83 weight portion Hirudo 250 weight portion Radix Glycyrrhizae 83 weight portions.

Chinese medicine composition of the present invention is comprised of kinds of traditional Chinese medicines such as the Radix Astragali, Flos Lonicerae, Cortex Phellodendri, Rhizoma Atractylodis, Semen Coicis, Radix Scrophulariae, Radix Angelicae Sinensis, the Radix Paeoniae Alba, Radix Glycyrrhizae, Hirudo, Scorpio, Scolopendras, the effect that in side, the Radix Astragali, Semen Coicis, Radix Angelicae Sinensis have benefiting QI for strengthening the superficies, enrich blood and invigorate blood circulation, Flos Lonicerae, Cortex Phellodendri, Radix Scrophulariae, Rhizoma Atractylodis have the effect of heat clearing and damp drying, eliminating fire and detoxication, the Radix Paeoniae Alba, Scorpio, Scolopendra have the effect of relieving spasm by subduing liver-wind, dispersing pathogen accumulation, removing obstruction in the collateral to relieve pain, the effect that Hirudo has removing blood stasis, stimulates the menstrual flow.All medicines coordinate, and have strengthened the merit of heat clearing and damp drying, blood circulation promoting and blood stasis dispelling, reducing swelling and alleviating pain, and pharmacology pharmacodynamic is tested and shown, Chinese medicine composition of the present invention all has good therapeutic effect to various types of hemorrhoid.

Another object of the present invention is to provide the preparation method of above-mentioned Chinese medicine composition.Using Chinese medicine of the present invention as raw material, add the different auxiliary material of pharmaceutically often using, adopt common process, medicine of the present invention can be prepared into different clinical medicine dosage forms, as pill, granule, capsule, tablet, oral liquid, masticatory, drop pill or Emulsion.

The conventional medicine excipient that medicine of the present invention adds as required can disintegrating agent, one or more in the middle of dispersant, lubricant, diluent, antiseptic, coloring agent, correctives etc.

A kind of preparation method of preparing above-mentioned Chinese medicine composition comprises: by Hirudo, Scolopendra, three kinds of animal drugs of Scorpio through cleaning, after washing, sterilizing, dried, through various superfine communication techniques of the prior art, carry out micronizing, make medicated powder mean diameter be less than 75 μ m, by Flos Lonicerae, Rhizoma Atractylodis, Radix Scrophulariae, Radix Angelicae Sinensis, the Radix Paeoniae Albas etc. first adopt routine techniques extracting in water volatile oil, medicinal residues and the Radix Astragali, Cortex Phellodendri, Semen Coicis, Radix Glycyrrhizae decocts with water extraction together, water extraction liquid filters by concentrated, adding ethanol turns molten essence and carries, reconcentration becomes the water extracted immersing paste, remain 1/2 recipe quantity Hirudo, Scolopendra, Scorpio superfine powder is first uniformly mixed and adds appropriate granulation liquid mix and blend to granulate again with the water extracted immersing paste, dry, granulate, spray into Flos Lonicerae, Rhizoma Atractylodis, Radix Scrophulariae, Radix Angelicae Sinensis, the volatile oil alcoholic solution that the Radix Paeoniae Alba is extracted, mix by particles packing machine and pack, granulation.

The another kind of preparation method of preparing above-mentioned Chinese medicine composition comprises: by Hirudo, Scolopendra, three kinds of animal drugs of Scorpio through cleaning, after washing, sterilizing, dried, through various superfine communication techniques of the prior art, carry out micronizing, make medicated powder mean diameter be less than 75 μ m; Flos Lonicerae, Rhizoma Atractylodis, Radix Scrophulariae, Radix Angelicae Sinensis, the Radix Paeoniae Alba etc. are first adopted to routine techniques extracting in water volatile oil, medicinal residues decoct with water extraction together with the Radix Astragali, Cortex Phellodendri, Semen Coicis, Radix Glycyrrhizae, water extraction liquid filters by concentrating, add ethanol and turns that molten essence is carried, reconcentration becomes the water extracted immersing paste, to remain 1/2 recipe quantity Hirudo, Scolopendra, Scorpio superfine powder and the water extracted immersing paste and appropriate granulation liquid mix and blend granulation, dry, granulate, spray into the volatile oil alcoholic solution of Flos Lonicerae, Rhizoma Atractylodis, Radix Scrophulariae, Radix Angelicae Sinensis, Radix Paeoniae Alba extraction, mix by particles packing machine and pack, granulation.

The third preparation method of preparing above-mentioned Chinese medicine composition comprises: by Hirudo, Scolopendra, three kinds of animal drugs of Scorpio through cleaning, after washing, sterilizing, dried, through various superfine communication techniques of the prior art, carry out micronizing, make medicated powder mean diameter be less than 75 μ m; Flos Lonicerae, Rhizoma Atractylodis, Radix Scrophulariae, Radix Angelicae Sinensis, the Radix Paeoniae Alba etc. are first adopted to routine techniques extracting in water volatile oil, medicinal residues decoct with water extraction together with the Radix Astragali, Cortex Phellodendri, Semen Coicis, Radix Glycyrrhizae, water extraction liquid filters by concentrating, add ethanol and turns that molten essence is carried, reconcentration becomes the water extracted immersing paste, extract dry is become to dry powder, standby; Remaining 1/2 recipe quantity Hirudo, Scolopendra, Scorpio superfine powder flour and the water extracted immersing paste granulates through the worth water extraction liquid extract dry powder of conventional drying process and appropriate granulation liquid mix and blend, dry, granulate, spray into the volatile oil of Flos Lonicerae, Rhizoma Atractylodis, Radix Scrophulariae, Radix Angelicae Sinensis, Radix Paeoniae Alba extraction, mix by particles packing machine and pack, granulation agent.

Most preferred dosage form of the present invention is pill, its preparation method comprises: extracting honeysuckle, Rhizoma Atractylodis, Radix Scrophulariae, Radix Angelicae Sinensis, the Radix Paeoniae Alba are placed in and in multi-function extractor, carry out steam distillation, obtain volatile oil crude product, redistillation, obtain refined volatile oil, prepare volatile oil betacyclodextrin clathrate, put in hermetic container and store; Getting the Hirudo, Scolopendra, Scorpio of the Radix Astragali, Cortex Phellodendri, Semen Coicis, Radix Glycyrrhizae and 1/2 recipe quantity and the medicinal residues after distillation decocts with water 1-3 time, each 1-3 hours, merge decocting liquid twice, filter, the medicinal liquid of getting after distillation merges with it concentrated, after cooling, add ethanol standing more than 12 hours, make it abundant precipitation; By the supernatant liquid filtering after precipitate with ethanol, reclaim ethanol, concentrating under reduced pressure becomes extractum, then puts in vacuum drying oven and dry, pulverize, and obtains extractum fine powder; The Hirudo of another 1/2 amount, Scolopendra, scorpion powder are broken into routine or micropowders, animal drugs fine powder, extractum fine powder, volatile oil betacyclodextrin clathrate and starch are mixed by a certain percentage, add ethanol to make soft material as wetting agent; Soft material is joined in medicine refining machine, refining 2-3 time, make soft material evenly softening, till soft durometer is applicable to shaping, the qualified soft material of refining is progressively joined and in pellet processing machine feed bin, starts to manufacture pill, qualified pill through polishing, shaping glazing, be dried, select ball and coating, finally by packer, pack, make pill.

Better dosage form of the present invention is granule, its preparation method comprises: extracting honeysuckle, Rhizoma Atractylodis, Radix Scrophulariae, Radix Angelicae Sinensis, the Radix Paeoniae Alba are placed in and in multi-function extractor, carry out steam distillation, obtain volatile oil crude product, redistillation, obtain refined volatile oil, add dehydrated alcohol, mix homogeneously, obtain ethanol volatile oil solution, put in hermetic container and store.The medicinal residues of getting after the Radix Astragali, Cortex Phellodendri, Semen Coicis, Radix Glycyrrhizae and 1/2 recipe quantity Hirudo, Scolopendra, Scorpio and distillation decoct with water 1-3 time, each 1-3 hour, merge decocting liquid twice, filter, the medicinal liquid of getting after distillation merges with it concentrated, after cooling, add ethanol standing more than 12 hours, make it abundant precipitation.By the supernatant liquid filtering after precipitate with ethanol, reclaim ethanol, concentrating under reduced pressure becomes extractum.Hirudo, Scolopendra, the scorpion powder of residue recipe quantity are broken into fine powder, medicated powder, extractum, Icing Sugar and dextrin are mixed by a certain percentage, add ethanol to make soft material as wetting agent, enter boiling type drying bed dry, spray volatile oil alcoholic solution, finally by conventional operation directly or add pharmaceutically acceptable excipient, granulation agent.

The present invention has the effect of heat clearing and damp drying, blood circulation promoting and blood stasis dispelling, reducing swelling and alleviating pain, pharmacology pharmacodynamic test shows, Chinese medicine composition of the present invention can reduce due to rat anus swelling due to Oleum Tiglii, acetic acid rat anus swelling due to rat Perianal ulceration and carrageenin, and the tissue injury at anal orifice and rectal intestine position is protected in the increase that reduces anus position capillary permeability; Can extend clotting time and prothrombin time, have obvious reduction plasma viscosity, improve hemorheologic parameters, improve significantly blood coagulation system, there is function of promoting blood circulation to disperse blood clots; Chinese medicine composition of the present invention can suppress the increase of the rat thoracic cavity WBC number that carrageenin causes, due on Carrageenan, rat paw edema all has obvious inhibitory action and antiinflammation, can extend the mouse writhing reaction that mice causes the sweet sufficient response latency of hot plate stimulation, inhibition acetic acid, extend the effect that writhing occurs incubation period, significantly suppresses the mice ear of dimethylbenzene induction, demonstrate certain antiinflammatory, analgesic activity.

Chinese medicine composition of the present invention can obviously improve the caused inflammation of hemorrhoid, pain symptom, eliminates the rectum position blood stasis of blood stagnant by activating blood circulation to dissipate blood stasis, thereby eliminates and produce hemorrhage influence factor from source of disease.For the therapeutical effect of checking medicine of the present invention to hemorrhoid, inventor has done a large amount of experimentatioies by multiple hemorrhoid model, by following experimental example, is used for further illustrating the therapeutic effect of Chinese medicine composition of the present invention to hemorrhoid.

The positive control medicine using in the following experimental example of the present invention, capsule for treating piles with 9-medicinal herbs is that Guizhou Wansheng Pharmaceutical Co., Ltd. produces, lot number is 110301, specification: every fills 0.4 gram, 15/plate X2 plate/box; The clever sheet stepping is that German gift reaches the production of large pharmaceutical factory, and product batch number is 7449, specification: every containing Aesculus hippocastanum extract 150mg, 20, every box; Dexibuprofen is prepared by the Drug Manufacturing Room of China Pharmaceutical Research & Development Center Co., Ltd, lot number: C102-1106017M.

Experimental example 1 Chinese medicine composition of the present invention causes the therapeutic effect of rat anus swelling model to Oleum Tiglii

1.1 Oleum Tigliis cause rat anus swelling model and make and index determining

Choose 100 of the SD rats that 180-220g is heavy, male and female half and half.Laboratory animal is divided into 5 groups at random according to body weight: model control group (gavage equal-volume distilled water), positive controls (capsule for treating piles with 9-medicinal herbs), high, medium and low 3 the dosage groups of Chinese medicine composition of the present invention, 20/group.Each group all adopts gastric infusion, dosage as shown in table 1, table 2 or table 3, administration 20ml/kg.1h after last administration, carries out light anaesthesia with ether to rat, will soak inhales the cotton swab of Oleum Tiglii, inserts anesthetized rat anal 10s, and the degree of depth should not be too dark, with 15mm, is advisable, and puts to death rat after 6h, before putting to death by rat anesthesia and measure following index:

(1) clotting time detects: use after chloral hydrate anesthesia, will get blood vessel and insert eye rear vein beard, along getting vessel flow, go out blood, drip one and drip on clean sheet glass, clock immediately with stopwatch.Every about 20s, with clean pin, from drop of blood edge, stir once gently inwards, and observation has or not the blood streak to provoke.From blood sampling, starting to only provoking the blood streak, is clotting time between institute lasts.

(2) prothrombin time PT detects: simultaneously by the blood collection of above-mentioned outflow in 1ml pipe, the sodium citrate anticoagulant (0.109mol/L) that has added 0.1ml in it, while being added to 1ml, build lid, by after blood mix homogeneously, the centrifugal 10min of 3000rpm, gets supernatant as for-20 ℃ of Refrigerator stores.Mensuration for prothrombin time PT.

(3) mensuration of rectal tissue: cut and be about 15mm rectum portion claim weight in wet base from anus fur edge, and be placed in after 80 ℃ of xeothermic 16h, take dry weight, calculate anorectum coefficient=weight in wet base/dry weight, as degree of inflammation index.

(4) vascular permeability detects.30min before sacrifice of animal, injects 2% azovan blue (EB) 20mg/kg (1ml/kg, 20mg/ml) through jugular vein, 0.5h is by superior mesenteric artery connecting tee pipe, take out most blood, pour into again about 10ml normal saline, go out blood and remaining EB in circulation at once.Get about 15mm from anus fur edge, cut off longitudinal tangent plane, cut off about 5mm wide, be placed in 10% formalin, fixing, carry out histopathologic examination.Remainder claims weight in wet base, according to the ratio of 1g:10ml, adds Methanamide, water-bath 24h in 50 ℃ of constant water bath box, and the centrifugal 10min of 3000rpm, gets supernatant and measures the absorbance OD value under 620nm with spectrophotometric.

1.2 experimental result

1.2.1 the impact of Chinese medicine composition of the present invention on rat anal orifice and rectal intestine weight

Experimental result shows, with model group comparison, 3 dosage groups of Chinese medicine composition of the present invention (7.2-14.4g/kg) significantly reduce weight in wet base (the P < 0.01 of rectum after Oleum Tiglii modeling, P < 0.01, P < 0.01), dry weight (P < 0.01, P < 0.01, P < 0.01), and significantly reduce weight in wet base/dry weight ratio (P < 0.05, P < 0.05, P < 0.05), show that Chinese medicine composition of the present invention can improve the rat anus swelling that Oleum Tiglii causes, alleviate inflammatory edema, improve hemorrhoid damage.The results are shown in Table 1.Under same experiment condition, positive control drug capsule for treating piles with 9-medicinal herbs also significantly reduces weight in wet base (P < 0.01), dry weight (P < 0.05) and weight in wet base/dry weight ratio (P < 0.05) of rectum after Oleum Tiglii modeling, has shown equally the significant hemorrhoid damaging action that improves.3 dosage groups of Chinese medicine composition of the present invention are better than capsule for treating piles with 9-medicinal herbs improving aspect These parameters, but there was no significant difference.

Table 1 Chinese medicine composition of the present invention on Oleum Tiglii modeling after the impact of rat anal orifice and rectal intestine weight

Note: * represents to compare P < 0.05, * * P < 0.01 with model group

1.2.2 the impact of Chinese medicine composition of the present invention on rat anal orifice and rectal intestine vascular permeability

Table 2 result shows, with model group comparison, Chinese medicine composition of the present invention obviously reduces OD value, high dose group (14.4g/kg) has significant difference (P < 0.05), show that Chinese medicine composition of the present invention has the effect of remarkable inhibition vascular permeability, and there is the effect of the rectal tissue inflammatory exudation that remarkable inhibition Oleum Tiglii causes.Under same experiment condition, positive control drug capsule for treating piles with 9-medicinal herbs also significantly reduces OD value (P < 0.05), has shown significant inhibition vascular permeability, and alleviates the effect of the rectal tissue inflammatory exudation that Oleum Tiglii causes.Two dosage groups of Chinese medicine composition (9.6g/kg and 14.4g/kg) are compared with capsule for treating piles with 9-medicinal herbs group has the difference of significance aspect inhibition vascular permeability.

Table 2 Chinese medicine composition of the present invention on Oleum Tiglii modeling after the impact of rat anal orifice and rectal intestine vascular permeability

Note: with model group comparison, * P < 0.05, * * P < 0.01; Compare with capsule for treating piles with 9-medicinal herbs group, ^#p < 0.05

1.2.3 the impact of Chinese medicine composition of the present invention on rat clotting time

Table 3 result shows, with model group comparison, Chinese medicine composition high dose group of the present invention (14.4g/kg) significance extends clotting time (P < 0.05) and prothrombin time (P < 0.05), show the Chinese medicine composition tool of the present invention blood coagulation system that is significantly improved, show and there is the effect of invigorating blood circulation.Under same experiment condition, positive control drug capsule for treating piles with 9-medicinal herbs has no significant change (P > 0.05) to clotting time and prothrombin time.

The impact of table 3 Chinese medicine composition of the present invention on rat clotting time and prothrombin time

Note: with model group comparison, * P < 0.05, * * P < 0.01

Experimental example 2 acetic acid cause the mensuration of rat Perianal ulceration model and thoracic cavity WBC

2.1 acetic acid cause the modelling of rat Perianal ulceration and index determining

Choose body weight 70 of the SD of 180-220g rats, male and female half and half, are divided into 7 groups at random by rat: Normal group, model group, capsule for treating piles with 9-medicinal herbs group, clever group advanced in years, Chinese medicine composition low dose group of the present invention, middle dosage group and high dose group.

Application method: the filter paper that is 6mm by equal and opposite in direction internal diameter launches and puts into glacial acetic acid and fully soaks, except Normal group, all rats are sticked to anus with the filter paper that is soaked with glacial acetic acid, filter paper need be close to anal skin and mucosa, 0.5min, 1min changes filter paper.Within the 2nd day, press body weight and ulcer level by rat random packet, administration is evaluated ulcer level according to rat ulcer healing state in the 3rd, 5,7,9,11,13 days.Evaluation criteria: crissum is rubescent, swelling 4 minutes, has ulcer sepage 3 minutes; A little ulcer sepage 2 minutes; Basic healing 1 minute; Healing (normally) is 0 minute completely.After sticking 99% glacial acetic acid filter paper, all there is white ulcer surface, crissum swelling and have inflammation to ooze out in each treated animal.

Successive administration 14d, dosage as mentioned above.Fasting 12h, during experiment after gastric infusion 1h, in Rat Right pleurobranch chamber, (lean on right back angle, thoracic cavity in order to avoid the damage lobe of the lung) as far as possible and inject sterilizing 1% carrageenin normal saline solution, 0.5ml/ only, after 5h, de-cervical vertebra is put to death rat, at diaphragm periphery, cut thoracic cavity (avoiding blood to infiltrate thoracic cavity) open and get pleural effusion 0.2ml with syringe, by numeration of leukocyte method, under inverted microscope, observe counting leukocyte.WBC method of counting is with reference in pharmacological experimental methodology 1248 pages, counting after Gentian Violet colour developing.

2.2 experimental result

Experimental result demonstration, all there is white ulcer surface, crissum swelling and has inflammation to ooze out in each treated animal after sticking glacial acetic acid filter paper.Along with passage of time, each is organized rat Perianal ulceration and can alleviate gradually voluntarily, but administration group rat ulcer healing rate is obviously accelerated.Result demonstration, model group is observed in administration 14d, and crissum situation progressively returns to normal by serious redness, and its appraisal result and Normal group relatively have the difference (P < 0.01) of significance, this model modeling success.All the other each group and model group comparisons, when the administration phase finishes, rat anus swelling have significant therapeutic effect (P < 0.01) due to Chinese medicine composition high dose Dichlorodiphenyl Acetate of the present invention.Wherein middle dosage and low dose group also have therapeutical effect.Under same experiment condition, the therapeutical effect of rat anus swelling is remarkable due to positive control drug capsule for treating piles with 9-medicinal herbs group (P < 0.05) and clever group of (P < 0.05) Dichlorodiphenyl Acetate advanced in years.The results are shown in Table 4.

Table 4 Chinese medicine composition Dichlorodiphenyl Acetate of the present invention causes the rat anal ulcer degree grade form of Perianal ulceration model

Note: with normal control comparison, #P < 0.05, ##P < 0.01; With model group comparison, * P < 0.05, * * P < 0.01

Crissum swelling impact due to experimental example 3 Chinese medicine composition on Carrageenan of the present invention

Due to 3.1 carrageenin, crissum swelling model is made and index determining

Get healthy SD rat, body weight 180-200g, 70, female half and half, by body weight, be divided at random 7 groups, 10 every group, Normal group, model group, capsule for treating piles with 9-medicinal herbs group, clever group, the basic, normal, high dosage group of Chinese medicine composition of the present invention stepping.Each organizes equal gastric infusion, dosage as shown in table 5 or table 6, successive administration 14 days, administration volume is 20ml/kg, Normal group and model group are to the 0.5%CMC of same volume.1h after administration in the 10th day wherein, except Normal group, after intraperitoneal anesthesia, to anal downward rectal mucosa seromuscular layer, inject 1% carrageenin 0.1mL, respectively at 6,24,48,72,96h surveys the diameter of whole perianal tissue swelling, get the average of its long and short two diameter, as the index of measured swelling degree, and sepage situation is recorded in perusal.After last 1 day administration 1h, rat, after chloral hydrate anesthesia, measures crissum swelling, observed and recorded sepage, gets blood measurement clotting time, surveys PT.

3.2 experimental result

3.2.1 the impact of rat anus swelling degree due to Chinese medicine composition on Carrageenan of the present invention

There is obvious perianal tissue edema after injecting 1% carrageenin in each treated animal anal, model group and normal control swelling degree have significant difference.And crissum swelling peaks during 24h after injection.Compare with model group, the rat crissum swelling that the equal on Carrageenan of the low middle high dose group of Chinese medicine composition of the present invention causes all has obvious inhibitory action, acts on inhibition the most obviously (P<0.01) when causing scorching rear 24-48h.Under same experiment condition, it is not good that the therapeutical effect of rat anus swelling is organized due on Carrageenan in positive control drug capsule for treating piles with 9-medicinal herbs group and the spirit stepping.

The impact of rat anus swelling model swelling degree due to table 5 Chinese medicine composition on Carrageenan of the present invention

3.2.2 the impact of rat clotting time and prothrombin time in rat anus swelling experiment due on Carrageenan

Result shows, with model group comparison, the basic, normal, high dosage group of Chinese medicine composition of the present invention significance extends clotting time and prothrombin time (P < 0.05), shows that Chinese medicine composition of the present invention has the effect of invigorating blood circulation, and can significantly improve blood coagulation system.Under same experiment condition, positive control drug capsule for treating piles with 9-medicinal herbs and the spirit stepping also have certain prolongation effect to clotting time and prothrombin time, but its effect is not as good as Chinese medicine composition of the present invention.

The impact of table 6 Chinese medicine composition of the present invention on the clotting time of anus swelling model and prothrombin time

Experimental example 4 Chinese medicine composition on Carrageenan of the present invention causes the impact of PGE2 in the impact of rat paw edema and blood and inflammation tissue

4.1 carrageenin cause rat paw edema modelling and index determining

Reference literature method, healthy SD rat, body weight 180-200g, 70, male and female half and half, are divided into 7 groups at random by body weight.Normal group, model group, capsule for treating piles with 9-medicinal herbs group (1.0g/kg), dexibuprofen group (0.02g/kg) and Chinese medicine composition of the present invention low (7.2g/kg), in (9.6g/kg), high dose group (14.4g/kg), 10 every group.Normal group and model group gavage are to 0.5%CMC.After last medicine, marking pen is ankle place labelling after Rat Right, mensuration causes scorching front Rat Right metapedes sole of the foot volume measuring apparatus and measures, record displacement (mL), then except Normal group, 1h after last administration, in Rat Right metapedes plantar subcutaneous injection, 1% carrageenin 0.1mL causes inflammation, measures and causes scorching rear 0.5h, 1h, 2h, 4h, 6h Rat Right metapedes volume.Calculate swelling rate, scorching front foot sole of the foot volume * 100% of swelling rate=(cause scorching metapedes sole of the foot volume-cause scorching front foot sole of the foot volume)/cause.

Mensuration to PGE2 in rat foot claw inflammatory exudate: carrageenin causes in the experiment of rat paw edema method, after measuring the paw swelling of rat, rat anesthesia is put to death, peeling, the scorching foot portions of clip also shreds, after weighing, in every 1g tissue, add 4ml normal saline, the lower 24h that soaks of 4 degree, centrifugal 3500 turn, 15min, get supernatant 0.1ml, add 05mol/LKOH-methanol solution 2ml, isomerization 20min(water-bath at 50 ℃), add again methanol 2ml, mix, in 278nm place, measure OD value, with every gram of inflammation, organize suitable OD value (A/g) to represent the content of PGE.

4.2 experimental result

4.2.1 the impact of rat paw edema degree due to Chinese medicine composition on Carrageenan of the present invention

Result demonstration, with normal control comparison, model group causes scorching rear swelling rate significant difference, modeling success.With model group comparison, the basic, normal, high dosage of Chinese medicine composition of the present invention all can be significantly or the utmost point suppress significantly rat paw edema percentage rate due on Carrageenan, show that Chinese medicine composition of the present invention has significant antiinflammation, and there is obvious dose-dependence in antiinflammation.Under same experiment condition, due to positive control drug capsule for treating piles with 9-medicinal herbs and dexibuprofen on Carrageenan, rat paw edema also has inhibitory action in various degree, the foot swelling inhibition of its foot swelling inhibition and Chinese medicine composition low dose group of the present invention is close, but the foot swelling inhibition of the middle and high dosage group of too late Chinese medicine composition of the present invention.

The percentile impact of rat paw edema due to table 7 Chinese medicine composition on Carrageenan of the present invention

4.2.2 the PGE2 impact in the scorching foot of rat due to Chinese medicine composition on Carrageenan of the present invention

Result demonstration, with Normal group comparison, the PGE2 discharging in the inflammation of model group animal foot is significantly higher than Normal group (P < 0.01).With model group comparison, the PGE2 discharging in the scorching foot of rat due to the middle and high dosage on Carrageenan of Chinese medicine composition of the present invention has extremely significant inhibitory action (P < 0.01), and the PGE2 discharging in the scorching foot of rat due to low dose group on Carrageenan also has significant inhibitory action (P < 0.05).Under same experiment condition, the PGE2 discharging in the scorching foot of rat due to positive control drug capsule for treating piles with 9-medicinal herbs and dexibuprofen (P < 0.05) on Carrageenan also has inhibitory action to some extent, but its inhibition is all not as good as Chinese medicine composition of the present invention.

PGE2 impact due to table 8 Chinese medicine composition on Carrageenan of the present invention in the scorching foot of rat

Experimental example 5 Chinese medicine composition xylol of the present invention causes the impact of mice ear

Reference literature method is got 72 of healthy ICR mices, ♀ ♂ half and half, minutes 6 groups, 12/group, i.e. model group, capsule for treating piles with 9-medicinal herbs, dexibuprofen group, Chinese medicine composition low dose group of the present invention, middle dosage group and high dose group.1 time/d of every ig administration; Administration 14d, 60min after last medicine, mouse right ear is evenly smeared dimethylbenzene 0.03mL/ and is only caused swollenly, cuts ears and beat auricle and weigh after 2h, calculates ear swelling value (mg).Swelling value=auris dextra weight-left ear weight.

Result shows, with model group comparison, the low middle Senior Three of a Chinese medicine composition of the present invention dosage group all can significantly reduce the ear swelling value (P < 0.01) of dimethylbenzene induced mice, show that Chinese medicine composition of the present invention has the effect of the mice ear of remarkable inhibition dimethylbenzene induction, and there is significant dose-effect relationship in its reduction effect to ear swelling.Under same experiment condition, positive control drug dexibuprofen group also significantly reduces ear swelling value (P < 0.05), but its antiphlogistic effects is obviously not as good as Chinese medicine composition of the present invention.

The impact of table 9 Chinese medicine composition xylol of the present invention induced mice ear swelling

Note: with model group comparison, * P < 0.05, * * P < 0.01

The impact of experimental example 6 Chinese medicine composition of the present invention on mice hot-plate analgesia

6.1 mice hot plate pain models are made

Body weight 20g left and right female mice is placed on hot plate dolorimeter, (licking metapedes time <5s or >30s throws aside to screen 90 of qualified ♀ mices, for preventing foot, scald, also should establish deadline, be generally 60s), be the threshold of pain index incubation period that the mice of take is added metapedes reaction, get 72 jennies, be divided into 6 groups, 12/group, i.e. model group, capsule for treating piles with 9-medicinal herbs, dexibuprofen group, Chinese medicine composition low dose group of the present invention, middle dosage group and high dose group.Each group is gastric infusion, and 1 times/day, dosage is as shown in table 10, after successive administration 14d, and the time that after mensuration last medicine, 60min, 120min add foot reaction.

6.2 Chinese medicine compositions of the present invention are on licking the impact of foot response latency in the experiment of mice hot-plate analgesia

Mice licks foot response latency time showing, with model group comparison, after administration after 1h and 2h, all be greater than model group the incubation period that the mice of each administration group of Chinese medicine composition of the present invention is added foot reaction, there is dose-dependence, and be significantly greater than model group (P < 0.05) incubation period of high dose group.Illustrate that Chinese medicine composition of the present invention can extend adding the sufficient response latency that mice stimulates hot plate, has certain analgesic activity.Under same experiment condition, positive control drug capsule for treating piles with 9-medicinal herbs group (P < 0.05) and dexibuprofen group (1h, P < 0.05; 2h, P < 0.01) also significant prolongation mice incubation period of adding foot reaction, have certain analgesic activity.The results are shown in Table 10.

Table 10 Chinese medicine composition of the present invention is on licking the impact of foot response latency in the experiment of mice hot-plate analgesia

Note: with model group comparison, * P < 0.05, * * P < 0.01

The impact of experimental example 7 Chinese medicine composition Dichlorodiphenyl Acetate of the present invention induced mice writhing

Get 72 of healthy ICR mices, ♀ ♂ half and half, is divided into 6 groups at random, and 12/group, i.e. model group, capsule for treating piles with 9-medicinal herbs, dexibuprofen group, Chinese medicine composition low dose group of the present invention, middle dosage group and high dose group.Model group gavage is to 0.5%CMC, and other each groups are to corresponding medicine, and dosage is as shown in table 11, continuously 14d.60min after last medicine, ip only injects 0.7% glacial acetic acid 0.2mL/, and in observed and recorded 15min there is incubation period, writhing number of times in mouse writhing immediately.

Result shows, with model group comparison, the basic, normal, high dosage group of Chinese medicine composition of the present invention can significantly suppress the mouse writhing reaction (P < 0.05) that acetic acid causes, and significant prolongation the writhing of mice there is incubation period, show that the pain that Chinese medicine composition of the present invention causes periphery chemical irritation has significant analgesic activity.Under same experiment condition, positive control drug capsule for treating piles with 9-medicinal herbs group and dexibuprofen group also have certain analgesic effect, but its analgesic effect is not as good as Chinese medicine composition of the present invention.

On writhing in mice acetic acid pain model, there is the impact of incubation period and writhing number of times in table 11 Chinese medicine composition of the present invention

Note: with model group comparison, * P < 0.05, * * P < 0.01

The impact of experimental example 8 Chinese medicine composition Dichlorodiphenyl Acetate induced mice of the present invention abdominal cavity capillary permeability

Get 72 of ICR mices, ♀ ♂ half and half, is divided into 6 groups, and 12/group, i.e. model group, capsule for treating piles with 9-medicinal herbs, dexibuprofen group, Chinese medicine composition low dose group of the present invention, middle dosage group and high dose group.Each organizes continuous gastric infusion 14d, dosage is as shown in table 12,60min after last medicine, and after mouse tail vein injection 0.5% azovan blue 0.1mL/10g, ip0.6% glacial acetic acid 0.2mL/ is only immediately, after 20min, ip normal saline 5mL/ only, gently rub abdominal part, draw neck to put to death, draw peritoneal fluid, 3000r/min, 10min, centrifugal after in 721 spectrophotometer 590nm place colorimetrics, respectively organize OD value.

Result demonstration, with model group comparison, the absorbance of the mouse peritoneal cleanout fluid of basic, normal, high three the dosage groups of Chinese medicine composition of the present invention is starkly lower than model group (P < 0.05).This shows that Chinese medicine composition of the present invention can suppress the increase of the mouse peritoneal capillary permeability that acetic acid causes, and presents certain dose-dependence.Under same experiment condition, positive control drug dexibuprofen group (P < 0.05) has also significantly suppressed the increase of the mouse peritoneal capillary permeability that acetic acid causes, but effect is not as good as Chinese medicine composition of the present invention.

The impact of table 12 Chinese medicine composition Dichlorodiphenyl Acetate induced mice of the present invention abdominal cavity capillary permeability

Note: with model group comparison, * P < 0.05, * * P < 0.01

The improvement effect of experimental example 9 Chinese medicine composition of the present invention to rat blood stasis models

Get healthy SD rat, body weight 180-200g, 70, male and female half and half, are divided into 7 groups at random by body weight: basic, normal, high group of Normal group, model group, capsule for treating piles with 9-medicinal herbs group, advanced in years clever group and Chinese medicine composition of the present invention, 10 every group.Gastric infusion, successive administration 14 days, Normal group and model group are to 0.5%CMC, and all the other give corresponding medicine, and dosage is as shown in table 14.Administration the 13rd day, 14 days, except Normal group, all the other treated animals are pressed 1mg/Kg subcutaneous injection adrenalin hydrochloride, and 2 times/day, interval 4h, after injection, 2h puts animal in 4 ℃ of frozen water 15min for the first time.Administration is got 4-5ml blood anticoagulant heparin on the 15th day and is measured blood rheology parameter, gets the liver conversion organ coefficient of weighing.

Result shows, model group and normal control comparison, and the low cut index of whole blood, erythrocyte aggregation index, Casson viscosity and plasma viscosity all have significantly and increase (P<0.05).With model group comparison, basic, normal, high each dosage group of the Chinese medicine composition of the present invention all remarkable or utmost point has reduced the low cut index of whole blood, erythrocyte aggregation index, Casson viscosity and plasma viscosity significantly, reduction amplitude amount effect relationship, wherein there were significant differences (P<0.05) for high dose.Under same experiment condition, positive control drug capsule for treating piles with 9-medicinal herbs group and the spirit stepping are organized and have also been reduced the high cut index of whole blood, the low cut index of whole blood, erythrocyte aggregation index, but it improves effect all not as good as Chinese medicine composition of the present invention to hemorheology index.The result statistics of liver index shows, model group and normal control comparison, and liver index significantly increases.And with model group comparison, basic, normal, high each dosage group of Chinese medicine composition of the present invention has significantly reduced liver index (P<0.01), positive control drug capsule for treating piles with 9-medicinal herbs and Chinese medicine composition of the present invention Chinese medicine composition high dose group of the present invention can reduce liver index (P<0.05) significantly, but effect is not as good as Chinese medicine composition of the present invention.

The impact that table 13 becomes rat blood stasis models blood flow

The impact of table 14 on rat blood stasis models liver coefficient

Note: with normal control comparison, ##P < 0.01; With model group comparison, * P < 0.05, * * P < 0.01

The specific embodiment

By specific embodiment, further illustrate the present invention below, but those skilled in the art should know, described embodiment does not also limit the present invention in any way.

Embodiment 1

Radix Astragali 300g Flos Lonicerae 300g Cortex Phellodendri 100g Rhizoma Atractylodis 100g Radix Scrophulariae 300g Radix Angelicae Sinensis 100g

Radix Paeoniae Alba 100g Radix Glycyrrhizae 50g Scolopendra 10g Scorpio 50g Hirudo 180g Semen Coicis 300g

Extracting honeysuckle, Rhizoma Atractylodis, Radix Scrophulariae, Radix Angelicae Sinensis, the Radix Paeoniae Alba are placed in multi-function extractor and carry out steam distillation, obtain volatile oil crude product, and redistillation, obtains refined volatile oil, adds dehydrated alcohol, and mix homogeneously obtains ethanol volatile oil solution, put in hermetic container and store.Getting the Hirudo, Scolopendra, Scorpio of the Radix Astragali, Cortex Phellodendri, Semen Coicis, Radix Glycyrrhizae and 1/2 recipe quantity and the medicinal residues after distillation decocts with water 2 times, time is respectively 1.5 hours and 1 hour, merge decocting liquid twice, filter, the medicinal liquid of getting after distillation merges with it concentrated, after cooling, add ethanol standing more than 12 hours, make it abundant precipitation.By the supernatant liquid filtering after precipitate with ethanol, reclaim ethanol, concentrating under reduced pressure becomes extractum.The Hirudo of another 1/2 amount, Scolopendra, scorpion powder are broken into fine powder, medicated powder, extractum, Icing Sugar and dextrin are mixed by a certain percentage, add ethanol to make soft material as wetting agent, enter boiling type drying bed dry, spray distillate, finally by conventional operation directly or add pharmaceutically acceptable excipient to make clinical acceptable dosage form, as pill, tablet, drop pill, Emulsion, masticatory, oral liquid, capsule, granule.

Embodiment 2

Radix Astragali 450g Flos Lonicerae 450g Cortex Phellodendri 200g Rhizoma Atractylodis 200g Radix Scrophulariae 450g Radix Angelicae Sinensis 200g

Radix Paeoniae Alba 200g Radix Glycyrrhizae 75g Scolopendra 20g Scorpio 80g Hirudo 220g Semen Coicis 450g

Embodiment 3

Radix Astragali 700g Flos Lonicerae 700g Cortex Phellodendri 400g Rhizoma Atractylodis 400g Radix Scrophulariae 700g Radix Angelicae Sinensis 400g

Radix Paeoniae Alba 400g Radix Glycyrrhizae 100g Scolopendra 40g Scorpio 100g Hirudo 280g Semen Coicis 700g

Embodiment 4

Radix Astragali 550g Flos Lonicerae 550g Cortex Phellodendri 300g Rhizoma Atractylodis 300g Radix Scrophulariae 550g Radix Angelicae Sinensis 300g

Radix Paeoniae Alba 320g Radix Glycyrrhizae 85g Scolopendra 25g Scorpio 80g Hirudo 250g Semen Coicis 550g

Embodiment 5

Radix Astragali 500g Flos Lonicerae 500g Cortex Phellodendri 250g Rhizoma Atractylodis 250g Radix Scrophulariae 500g Radix Angelicae Sinensis 250g

Radix Paeoniae Alba 250g Radix Glycyrrhizae 83g Scolopendra 20g Scorpio 83g Hirudo 250g Semen Coicis 500g

Embodiment 6

Radix Astragali 415g Flos Lonicerae 416g Cortex Phellodendri 208g Rhizoma Atractylodis 208g Radix Scrophulariae 416g Radix Angelicae Sinensis 208g

Radix Paeoniae Alba 208g Radix Glycyrrhizae 69g Scolopendra 16g Scorpio 69g Hirudo 208g Semen Coicis 416g

Extracting honeysuckle, Rhizoma Atractylodis, Radix Scrophulariae, Radix Angelicae Sinensis, the Radix Paeoniae Alba are placed in multi-function extractor and carry out steam distillation, obtain volatile oil crude product, and redistillation, obtains refined volatile oil, prepare volatile oil betacyclodextrin clathrate, put in hermetic container and store; Getting the Hirudo, Scolopendra, Scorpio of the Radix Astragali, Cortex Phellodendri, Semen Coicis, Radix Glycyrrhizae and 1/2 recipe quantity and the medicinal residues after distillation decocts with water 1-3 times, each 1-3 hours, merge decocting liquid twice, filter, the medicinal liquid of getting after distillation merges with it concentrated, after cooling, add ethanol standing more than 12 hours, make it abundant precipitation; By the supernatant liquid filtering after precipitate with ethanol, reclaim ethanol, concentrating under reduced pressure becomes extractum, then puts in vacuum drying oven and dry, pulverize, and obtains extractum fine powder; The Hirudo of another 1/2 amount, Scolopendra, scorpion powder are broken into routine or micropowders, animal drugs fine powder, extractum fine powder, volatile oil betacyclodextrin clathrate and starch are mixed by a certain percentage, add ethanol to make soft material as wetting agent; Soft material is joined in medicine refining machine, refining 2-3 time, make soft material evenly softening, till soft durometer is applicable to shaping, the qualified soft material of refining is progressively joined and in pellet processing machine feed bin, starts to manufacture pill, qualified pill through polishing, shaping glazing, be dried, select ball and coating, finally by packer, pack, make pill.

Embodiment 7

Radix Astragali 416.5g Flos Lonicerae 416.5g Cortex Phellodendri 208.5g Rhizoma Atractylodis 2088.5g Radix Scrophulariae 416.5g

Radix Angelicae Sinensis 208.5g Radix Paeoniae Alba 208.5g Radix Glycyrrhizae 69g Scolopendra 16.5g Scorpio 69g

Hirudo 208.5g Semen Coicis 416.g

Embodiment 8

Radix Astragali 500g Flos Lonicerae 500g Cortex Phellodendri 250g Rhizoma Atractylodis 250g

Radix Scrophulariae 500g Radix Angelicae Sinensis 250g Radix Paeoniae Alba 250g Semen Coicis 300g

Embodiment 9

Radix Paeoniae Alba 250g Scolopendra 20g Scorpio 83g Hirudo 250g Semen Coicis 500g

Embodiment 10

Prescription is with the embodiment of the present invention 2

A micronizing technique: through cleaning, after washing, sterilizing, dried, utilize preferred micronizing unit to carry out micronizing, make medicated powder mean diameter be less than 10 μ m the Hirudo of 1/2 recipe quantity, Scolopendra, three kinds of animal drugs of Scorpio;

B extracts concentrated and drying process: by Flos Lonicerae, Rhizoma Atractylodis, Radix Scrophulariae, Radix Angelicae Sinensis, the first extracting in water volatile oil of the Radix Paeoniae Alba, medicinal residues decoct with water extraction together with the Radix Astragali, Cortex Phellodendri, Semen Coicis, Radix Glycyrrhizae, and water extraction liquid filters by concentrating, add ethanol and turns that molten essence is carried, reconcentration becomes the water extracted immersing paste;

C Extraction Process of Volatile Oil: extracting honeysuckle, Rhizoma Atractylodis, Radix Scrophulariae, Radix Angelicae Sinensis, the Radix Paeoniae Alba are placed in multi-function extractor and carry out steam distillation, obtain volatile oil crude product, redistillation, obtain refined volatile oil, add dehydrated alcohol, mix homogeneously, obtain ethanol volatile oil solution, put in hermetic container and store.

Granulating process: granulate different according to the extract state after extracting, be divided into three kinds of method of granulating, a kind of is that residue 1/2 recipe quantity Hirudo, Scolopendra, Scorpio superfine powder and the water extracted immersing paste and appropriate granulation liquid mix and blend are granulated, dry, granulate, the volatile oil alcoholic solution that sprays into Flos Lonicerae, Rhizoma Atractylodis, Radix Scrophulariae, Radix Angelicae Sinensis, Radix Paeoniae Alba extraction, mixes by particles packing machine and packs, granulation; Another kind is that residue 1/2 recipe quantity Hirudo, Scolopendra, Scorpio superfine powder are first uniformly mixed and add appropriate granulation liquid mix and blend to granulate again with the water extracted immersing paste, dry, granulate, spray into the volatile oil alcoholic solution of Flos Lonicerae, Rhizoma Atractylodis, Radix Scrophulariae, Radix Angelicae Sinensis, Radix Paeoniae Alba extraction, mix by particles packing machine and pack, granulation; Another kind is that residue 1/2 recipe quantity Hirudo, Scolopendra, Scorpio superfine powder flour and the water extracted immersing paste are granulated through the worth water extraction liquid extract dry powder of conventional drying process and appropriate granulation liquid mix and blend, dry, granulate, spray into the volatile oil of Flos Lonicerae, Rhizoma Atractylodis, Radix Scrophulariae, Radix Angelicae Sinensis, Radix Paeoniae Alba extraction, mix by particles packing machine and pack, granulation agent.

Embodiment 11

Prescription is with the embodiment of the present invention 4.

A through cleaning, after washing, sterilizing, dried, utilize preferred micronizing unit to carry out micronizing, makes medicated powder mean diameter be less than 125 μ m Hirudo, Scolopendra, three kinds of animal drugs of Scorpio;

B extracts concentrated and drying process and C granulating process with the corresponding technique of embodiment 9.

Embodiment 12

Prescription is with the embodiment of the present invention 5.

A through cleaning, after washing, sterilizing, dried, utilize preferred micronizing unit to carry out micronizing, makes medicated powder mean diameter be less than 55 μ m Hirudo, Scolopendra, three kinds of animal drugs of Scorpio;

Embodiment 13

Prescription is with the embodiment of the present invention 5.

A through cleaning, after washing, sterilizing, dried, utilize preferred micronizing unit to carry out micronizing, makes medicated powder mean diameter be less than 28 μ m Hirudo, Scolopendra, three kinds of animal drugs of Scorpio;

Embodiment 14

Prescription is with the embodiment of the present invention 5.

Embodiment 15

Prescription is with the embodiment of the present invention 9.

A through cleaning, after washing, sterilizing, dried, utilize preferred micronizing unit to carry out micronizing, makes medicated powder mean diameter be less than 75 μ m Hirudo, Scolopendra, three kinds of animal drugs of Scorpio;

Embodiment 16

Prescription is with the embodiment of the present invention 7.

A through cleaning, after washing, sterilizing, dried, utilize preferred micronizing unit to carry out micronizing, makes medicated powder mean diameter be less than 30 μ m Hirudo, Scolopendra, three kinds of animal drugs of Scorpio;

Claims

1. the purposes of Chinese medicine composition in preparing medicine for treaching hemorrhoie, is characterized in that, contains following Chinese medicinal components in described Chinese medicine composition:

Radix Paeoniae Alba 100-400 weight portion Semen Coicis 300-700 weight portion.

2. purposes as claimed in claim 1, is characterized in that, also contains following Chinese medicinal components in described Chinese medicine composition:

3. purposes as claimed in claim 2, is characterized in that, contains following Chinese medicinal components in described Chinese medicine composition:

Radix Astragali 400-550 weight portion Flos Lonicerae 400-550 weight portion Cortex Phellodendri 200-300 weight portion

Rhizoma Atractylodis 200-300 weight portion Radix Scrophulariae 400-550 weight portion Radix Angelicae Sinensis 200-300 weight portion

Radix Paeoniae Alba 200-280 weight portion Semen Coicis 400-550 weight portion Scolopendra 10-40 weight portion

4. purposes as claimed in claim 3, is characterized in that, contains following Chinese medicinal components in described Chinese medicine composition:

5. purposes as claimed in claim 3, is characterized in that, contains following Chinese medicinal components in described Chinese medicine composition:

The Radix Astragali 416.5 weight portion Flos Lonicerae 416.5 weight portion Cortex Phellodendri 208.5 weight portions

Rhizoma Atractylodis 208.5 weight portion Radix Scrophulariae 416.5 weight portion Radix Angelicae Sinensis 208.5 weight portions

The Radix Paeoniae Alba 208.5 weight portion Semen Coicis 416.5 weight portion Scolopendra 16.5 weight portions

Scorpio 69 weight portion Hirudo 208.5 weight portion Radix Glycyrrhizae 69 weight portions.

6. the purposes as described in claim 1-5 any one, is characterized in that, described Chinese medicine composition is oral drug preparation.

7. purposes as claimed in claim 6, is characterized in that, described Chinese medicine composition oral drug preparation is one or more in its pill, granule, capsule, tablet, oral liquid, masticatory, drop pill and Emulsion

8. the purposes as described in claim 1-5 any one, is characterized in that the preparation method of described Chinese medicine composition comprises the steps:

Extracting honeysuckle, Rhizoma Atractylodis, Radix Scrophulariae, Radix Angelicae Sinensis, the Radix Paeoniae Alba are placed in multifunctional extracting pot and carry out steam distillation, obtain volatile oil crude product, and redistillation, obtains refined volatile oil, adds dehydrated alcohol, and mix homogeneously obtains ethanol volatile oil solution, put in hermetic container and store; The medicinal residues of getting after the Radix Astragali, Cortex Phellodendri, Semen Coicis, Radix Glycyrrhizae and 1/2 recipe quantity Hirudo, Scolopendra, Scorpio and distillation decoct with water 1-3 time, each 1-3 hour, merge decocting liquid twice, filter, the medicinal liquid of getting after distillation merges with it concentrated, after cooling, add ethanol standing more than 12 hours, make it abundant precipitation; By the supernatant liquid filtering after precipitate with ethanol, reclaim ethanol, concentrating under reduced pressure becomes extractum.Hirudo, Scolopendra, the scorpion powder of residue recipe quantity are broken into fine powder, medicated powder, extractum, Icing Sugar and dextrin are mixed by a certain percentage, add ethanol to make soft material as wetting agent, enter boiling type drying bed dry, spray volatile oil alcoholic solution, finally by conventional operation directly or add pharmaceutically acceptable excipient to make clinical acceptable dosage form.

9. the purposes as described in claim 1-5 any one, is characterized in that the preparation method of described Chinese medicine composition comprises the steps:

10. the purposes as described in as arbitrary in claim 1-5, is characterized in that the preparation method of described Chinese medicine composition comprises the steps:

Through cleaning, after washing, sterilizing, dried, carry out micronizing through various superfine communication techniques of the prior art, make medicated powder mean diameter be less than 75 μ m Hirudo, Scolopendra, three kinds of animal drugs of Scorpio, by Flos Lonicerae, Rhizoma Atractylodis, Radix Scrophulariae, Radix Angelicae Sinensis, the Radix Paeoniae Albas etc. first adopt routine techniques extracting in water volatile oil, medicinal residues and the Radix Astragali, Cortex Phellodendri, Semen Coicis, Radix Glycyrrhizae decocts with water extraction together, water extraction liquid filters by concentrated, adding ethanol turns molten essence and carries, reconcentration becomes the water extracted immersing paste, remain 1/2 recipe quantity Hirudo, Scolopendra, Scorpio superfine powder is first uniformly mixed and adds appropriate granulation liquid mix and blend to granulate again with the water extracted immersing paste, dry, granulate, spray into Flos Lonicerae, Rhizoma Atractylodis, Radix Scrophulariae, Radix Angelicae Sinensis, the volatile oil alcoholic solution that the Radix Paeoniae Alba is extracted, mix by particles packing machine and pack, granulation.

11. purposes as described in as arbitrary in claim 1-5, is characterized in that the preparation method of described Chinese medicine composition comprises the steps:

Through cleaning, after washing, sterilizing, dried, carry out micronizing through various superfine communication techniques of the prior art, make medicated powder mean diameter be less than 75 μ m Hirudo, Scolopendra, three kinds of animal drugs of Scorpio; Flos Lonicerae, Rhizoma Atractylodis, Radix Scrophulariae, Radix Angelicae Sinensis, the Radix Paeoniae Alba etc. are first adopted to routine techniques extracting in water volatile oil, medicinal residues decoct with water extraction together with the Radix Astragali, Cortex Phellodendri, Semen Coicis, Radix Glycyrrhizae, water extraction liquid filters by concentrating, add ethanol and turns that molten essence is carried, reconcentration becomes the water extracted immersing paste, to remain 1/2 recipe quantity Hirudo, Scolopendra, Scorpio superfine powder and the water extracted immersing paste and appropriate granulation liquid mix and blend granulation, dry, granulate, spray into the volatile oil alcoholic solution of Flos Lonicerae, Rhizoma Atractylodis, Radix Scrophulariae, Radix Angelicae Sinensis, Radix Paeoniae Alba extraction, mix by particles packing machine and pack, granulation.

12. purposes as described in as arbitrary in claim 1-5, is characterized in that the preparation method of described Chinese medicine composition comprises the steps:

Through cleaning, after washing, sterilizing, dried, carry out micronizing through various superfine communication techniques of the prior art, make medicated powder mean diameter be less than 75 μ m Hirudo, Scolopendra, three kinds of animal drugs of Scorpio; Flos Lonicerae, Rhizoma Atractylodis, Radix Scrophulariae, Radix Angelicae Sinensis, the Radix Paeoniae Alba etc. are first adopted to routine techniques extracting in water volatile oil, medicinal residues decoct with water extraction together with the Radix Astragali, Cortex Phellodendri, Semen Coicis, Radix Glycyrrhizae, water extraction liquid filters by concentrating, add ethanol and turns that molten essence is carried, reconcentration becomes the water extracted immersing paste, extract dry is become to dry powder, standby; Remaining 1/2 recipe quantity Hirudo, Scolopendra, Scorpio superfine powder flour and the water extracted immersing paste granulates through the worth water extraction liquid extract dry powder of conventional drying process and appropriate granulation liquid mix and blend, dry, granulate, spray into the volatile oil of Flos Lonicerae, Rhizoma Atractylodis, Radix Scrophulariae, Radix Angelicae Sinensis, Radix Paeoniae Alba extraction, mix by particles packing machine and pack, granulation agent.