CN103918801A - Application of ulmus macrocarpa seed oil to prepare anti-fatigue blend oil - Google Patents
Application of ulmus macrocarpa seed oil to prepare anti-fatigue blend oil Download PDFInfo
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- CN103918801A CN103918801A CN201310217439.5A CN201310217439A CN103918801A CN 103918801 A CN103918801 A CN 103918801A CN 201310217439 A CN201310217439 A CN 201310217439A CN 103918801 A CN103918801 A CN 103918801A
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Abstract
The invention provides application of ulmus macrocarpa seed oil to prepare anti-fatigue blend oil. Through experiments, the optimum CO2 supercritical extraction conditions for ulmus macrocarpa seed oil are determined as the pressure of 30 MPa, the temperature of 40 DEG C and the extraction time of 90 min. Ulmus macrocarpa seed oil is taken as a main material, one or more of other oil materials are taken as auxiliary materials, and the blend oil is prepared according to a certain ratio. In the experiments, xanthoceras sorbifolia oil and corn oil are determined as the raw materials, and the preferable ratio of xanthoceras sorbifolia oil to corn oil is 1:1-1:10. Through animal experiments for verifying health-care capability of ulmus macrocarpa seed oil, the ulmus macrocarpa seed oil disclosed by the invention is transparent in color and lustre and rich in fragrance, and has effects of resisting fatigue and resisting oxidation.
Description
Technical field
The present invention relates to the application of yellow elm seed oil in preparation antifatigue ready-mixed oil, field is manufactured in function of dominant food processing.
Background technology
Overtiredly refer to because the working time is long, labour intensity is excessive, psychological pressure is overweight causes used-up sub-health state.The hidden danger of its maximum is to cause that the hiding disease of health worsens rapidly, such as causing the underlying diseases such as hypertension to worsen acute causing circulatory obstacles such as causing cerebrovascular disease or cardiovascular disease, even occurs fatal symptom.Namely " overworking death " of the sudden death of bringing out after this long-term chronic fatigue.It is bad that people often works overtime, stays up late, has a rest, and the time has been grown and will cause anxiety, insomnia, failure of memory, mental depression, even causes depression and schizophrenia.If this fatigue continues 6 months or the longer time, just may there is the symptoms such as low fever, abscess of throat, decreased attention, failure of memory in health.And very serious chronicity fatigue is exactly probably the tendency of other illnesss.
Yellow elm (Ulmus macrocarpa Hance var.mongolica) has another name called wych-elm, large fruit elm, is racemosus shrub or the dungarunga of Ulmaceae Elm, high more than 10 meters.Sprig dark brown, has pubescence and sheet suberin projection.Leaf is avette, slightly thick, and edge is coarse heavy sawtooth, rough surface, back side hairiness.5-9 cluster of flower.Samara obovate, has short fine, soft fur.Be born in and fix that sand dune, produce Keerqin sandy land and muddy Starch 1500 gram sand ground.Be distributed in northeast, North China, Shaanxi and Anhui.Korea, Mongolia, the Soviet Union also have.
As a kind of desert plant, the resistance to sand of its drought resisting, the ecological value is huge; Its tree-like grace, has ornamental value; Wooden hard, be first-class high-quality timber; Be used as medicine and can control the diseases such as ascariasis, there is higher medical value.Yellow elm seed oil expression is edible.
Have no the yellow elm seed of clinical report oil and there is antifatigue, oxidation resistant function.
Summary of the invention
The object of this invention is to provide a kind of new purposes of yellow elm seed oil, i.e. the application of yellow elm seed oil in the anti-oxidant ready-mixed oil of preparation.
The yellow elm seed oil using in the new purposes of the present invention can prepare by various mode, such as: solvent extraction, aqueous enzymatic extraction, ultrasonic wave auxiliary extraction, microwave auxiliary extraction, cold-press extraction, supercritical carbon dioxide extracting etc., be preferably supercritical carbon dioxide extracting.
The preferred extraction conditions of method of the yellow elm seed of above-mentioned supercritical carbon dioxide extracting oil is: pressure 30-40Mpa, temperature are that 30-50 DEG C, extraction time are 90-180 minute;
More preferably: pressure 30Mpa, temperature are that 40 DEG C, extraction time are 180 minutes.
In the new purposes of the present invention, the main oil plant of preparing ready-mixed oil is yellow elm seed oil, and auxiliary oil plant is one or more in peanut oil, soybean oil, olive oil, rapeseed oil or corn oil; Auxiliary oil plant is preferably corn oil; The two ratio of yellow elm seed oil and corn oil is preferably 1; 1-1: 10.
Tired can having a certain impact to human body, particularly can there are some changes as indexs such as MDA (MDA), superoxide dismutase (SOD), lactic acid (LD), glutathione reductase (GR), glycogen, urea nitrogens (BUN) in the factor of some oxidative stress in body, be called anti-oxidant to the improvement of these indexs, therefore, the new purposes of the present invention, is preferably the application of yellow elm seed oil in the anti-oxidant ready-mixed oil of preparation.
In order to confirm yellow elm seed oil antifatigue and oxidation resistant function, inventor has carried out following experiment:
1, experiment material
1.1 test sample
Title: yellow elm seed oil samples;
The unit of providing: Beijing Agricultural College;
Proterties: yellow oily liquid;
Dissolubility: water insoluble;
Preservation condition: cool place, shading, sealing
Compound method: high dose is directly applied crude oil, in, salad oil dilution preparation for low dose group, used time agitator mixes.Sealing, 4 DEG C of refrigerator storages.For gastric infusion, the used time fully shakes up, and each administration residue liquid is discarded.
1.2 positive control drug
Lentinan film: Puluokangyu Natural Drug Co., Ltd., Zhejiang.Lot number: 101002, specification: 0.1g/ sheet, 36/plate, 1 plate/bag.Clinical administration mode is oral, one time 2～5, every day 2 times.Clinical practice is in replenishing qi to invigorate the spleen, qi-restoratives righting.Product authentication code: the accurate word Z20026215 of traditional Chinese medicines, shelf-life: 24 months.Compound method: use mortar pulverize, distilled water preparation, used time agitator mixes.Sealing, 4 DEG C of refrigerator storages.For gastric infusion, the used time fully shakes up, and each administration residue liquid is discarded.
1.3 reagent
Malondialdehyde Kit (MDA): Bioengineering Research Institute's product is built up in Nanjing, lot number: 20110802; Superoxide dismutase kit (SOD): Bioengineering Research Institute's product is built up in Nanjing, lot number: 20110729; Lactic acid Kit (LD): Bioengineering Research Institute's product is built up in Nanjing, lot number: 20110721; Glutathione reductase kit (GR): Bioengineering Research Institute's product is built up in Nanjing, lot number: 20110729; Glycogen kit: Bioengineering Research Institute's product is built up in Nanjing, lot number: 20110803; Determination of urea nitrogen kit (BUN): ShangHai RongSheng Biology Pharmacy Co., Ltd's product, lot number: 20101001; 4 DEG C of refrigerator storages of above reagent, for measuring serum and liver sample.
Physiological saline: the healthy and free from worry pharmaceutcal corporation, Ltd in Shandong, lot number: E10110809, the date of manufacture: 2010/11/08, valid until: 2012/05/07, normal temperature sealing storage, directly applies as the solvent of the required solution of reagent preparation box.
1.4 laboratory apparatus
UV-1601PC type visible ultraviolet spectrophotometer: Japanese Shimadzu company product;
Thunder Du Chemray360 automatic clinical chemistry analyzer: Shenzhen thunder Du life science Tou Fen Co., Ltd product;
The single-row thermostat water bath of HD type, Constant Temp. Instrument Factory, Liaoyang manufactures;
The conventional equipment of dissecting;
Micro sample adding appliance, plastics suction nozzle, graduated cylinder
1.5 animal used as test
Germline: ICR mouse, SPF level;
Sex, quantity: male, 90
Body weight, week age scope: starting weight 17.9 ± 1.0g, 6w, body weight 22.9 ± 1.2g when administration, 6～7w.
Source: be purchased from Beijing Vital River Experimental Animals Technology Co., Ltd.
Grade: SPF level
Animal production licence number: SCXK (capital) 2006-0009
Issuing unit: Science and Technology Commission of Beijing
Feeding environment condition: barrier environment system: be applied to SPF level mouse and raise; Occupancy permit number: SYXK (capital) 2008-0024; Humiture: 20～25 DEG C of temperature, relative humidity 40～70%; Rate of ventilation: 10～20 times/hour; Lighting hours: alternately illumination of 12h/12h every day; Cleaning and sterilization: duration of test sterilize every day ground, table top and metope, disinfectant is that benzalkonium bromide, lysol, ethanol are used alternatingly.
Feed: mouse maintains pellet, meets GB14924.3-2001 standard, is purchased from the feed corporation,Ltd that pulls together of Beijing Australia of section, and production licence number: SCXK (capital) 2009-0012 uses after high temperature and high pressure steam sterilizing, freely absorbs.
Drinking water kind: conventional environment system is used running water, and barrier environment system SPF level animal is drunk front high pressure steam sterilization water.Method of supplying water: freely absorb by drinking water for animals bottle.
2, test method
2.1 animal grouping and identifications
Labeling method is picric acid decoration method, in conjunction with group numbering, sex, cage tool numbering and individual numbering, all animals is numbered, and number of animals order be " group alias+sex (hero)+cage number+individual numbering ".Concrete group numbering, sex, cage tool numbering and individual numbering are in table 1.
The labeling method of test cage: after grouping, carry out group differentiation with different colours cage card, and indicate the information such as thematic title, animal strain, grade group, quantity, cage number, sex, animal number, responsible person, experimenter, date of test.
The packet marking of table 1 mice burden swimming experiment
2.2 method of administration
According to clinical plan method of administration, all take gastric infusion mode.
2.3 dosage designs
Tested material: yellow elm seed oil, in crude content, set low 2.5ml/kg.bw/d, middle 5.0ml/kg.bw/d, tri-dosage groups of high 10ml/kg.bw/d.
Lentinan: clinical administration mode is oral, determines that mouse oral dose is about 300mg/kg.bw/d.
2.4 operating procedure
With 90 of above-mentioned qualified ICR mouse, body weight 18～22g, male, be divided at random 5 groups by body weight, 12 every group: blank group, positive controls (lentinan film 300mg/kg), yellow elm seed innage, in, low dose group (with crude content count 2.5,5.0,10ml/kg.bw/d).Each administration group respectively gavage gives relative medicine, and control group is to equivalent salad oil 10ml/kg.bw/d, every day gastric infusion once, 28d continuously.
After successive administration 28d, after last administration 1h, test, at the sheet lead of mouse tail heavy burden 5%, dropping into water temperature is 27 ± 0.5 DEG C, the swimming trunk went swimming of depth of water 40cm, can not swim out of the water surface taking head submerged and exhaust (head sinks under water till 10s) as muscle power, the observed and recorded time that extremely muscle power is exhausted from drop into water is swimming time.After above mouse swimming, take out, eyeball blood sampling, the centrifugal 15min of 2000rpm/min after blood clotting, get serum frozen, and get liver, get three parts and weighings (requiring scale liver weight according to glycogen content, MDA, tri-kit description differences of SOD) for every, be placed in advance in ready pipe frozen.
Testing index: survey BUN, LD, GR with serum; Survey hepatic glycogen content, MDA, SOD with liver.Wherein serum BUN detects with automatic clinical chemistry analyzer, other index kit measurements, and by specification is surveyed after absorbance OD value, then calculates to specifications each index.
2.5 data statistics processing methods
Each administration group data are calculated
the DAS software that adopts the chief editors such as Sun Ruiyuan, Zheng Qingshan, carries out statistical analysis, with the t inspection of solvent group comparison, judges significant difference.
3, result of the test
As shown in Table 2, with the comparison of blank group, yellow elm seed oil high dose group mice burden swimming time significant prolongation (P < 0.01), under same experimental condition, positive control medicine lentinan film 300mg/kg highly significant extends (P < 0.01), shows that yellow elm seed oil sample all can improve mouse fatigue resistance;
By table 4, 5, 6, 7 is known, with the comparison of blank group, the yellow elm seed oil sample hepatic glycogen content (P < 0.05) in liver organization that significantly raises after mouse movement, significantly rising liver organization SOD activity (P < 0.05), significantly GR activity (P < 0.05) in rising serum, significantly reduce LD in serum, significantly reduce liver organization MDA content (P < 0, 05), under same experimental condition, the positive control medicine lentinan film 300mg/kg hepatic glycogen content (P < 0.01) in liver organization that also significantly raises after mouse movement, significantly GR activity (P < 0.05) in rising serum, significantly reduce LD in serum, significantly reduce liver organization MDA content (P < 0.05), show that yellow elm seed oil sample is close with lentinan, all can improve oxidation resistance and the anti-fatigue ability of exercised rats, reduce the damage of the damage factor producing in body to body.
The impact of the yellow elm seed of table 2 oil on the mice burden swimming time
With control group comparison, * P < 0.05, * * P < 0.01.
The yellow elm seed of table 3 oil on mouse movement after the impact of hepatic glycogen content
With control group comparison, * P < 0.05, * * P < 0.01.
The yellow elm seed of table 4 oil on mouse movement after the impact of serum LD content
With control group comparison, * P < 0.05, * * P < 0.01.
The yellow elm seed of table 5 oil on mouse movement after the impact of serum BUN content
With control group comparison, * P < 0.05, * * P < 0.01.
The yellow elm seed of table 6 oil on mouse movement after the impact of serum GR content
With control group comparison, * P < 0.05, * * P < 0.01.
The yellow elm seed of table 7 oil on mouse movement after the impact of MDA and SOD activity in liver organization
With control group comparison, * P < 0.05, * * P < 0.01.
4, experiment conclusion
Yellow elm seed oil can improve oxidation resistance and the anti-fatigue ability of exercised rats, reduces the damage of the damage factor producing in body to body.
This Huang elm seed oil can reduce the content of the interior lactic acid of the rear animal body of motion and MDA, extends the swimming with a load attached to the body time, improves the rear glutathione-reductase of motion, hepatic glycogen content, illustrates that it has certain antifatigue and antioxidation.
At present, the method for extracting grease from vegetable seeds has solvent refluxing extraction method, cold-maceration, hot moulding method, supercritical carbon dioxide extraction method, ultrasonic extraction etc.But consider from extraction efficiency, product purity, dissolvent residual problem equal angles, that supercritical carbon dioxide extraction technology has is easy to operate, energy consumption is low, extraction efficiency is high, product purity advantages of higher, oneself is especially well used through being applied to the field such as biological medicine, food in grease extracts.
In order to obtain the optimum process condition of the yellow elm seed of supercritical carbon dioxide extraction oil, inventor has carried out following experiment:
1, test material and instrument
1.1 experiment material
Yellow elm seed: pick up from Jilin Province's national level to the yellow Yushulin of extra large nature reserve area Mongolia (yellow Yulin 5-6 month of protection zone, Horqin, Inner Mongol (general 2 years once fruits of knot of this tree, the ripe 5-6 month of fruit).
1.2 test apparatus
Supercritical carbon dioxide extracting instrument: Applied Separations company of the U.S., carbon dioxide food grade 99.999%: northern wet body manufactory, precise electronic balance.Shanghai Second Balance Factory; High speed Universalpulverizer FW100 type: Tianjin Stettlen Instrument Ltd.; DH-101 electric heating constant-temperature blowing drying box: Tianjin Zhong Huan experimental electric furnace Co., Ltd.
2, test method
Get the yellow elm seed after cleaning impurity elimination, in drying box, after 105 DEG C of baking 2h, pulverize (crossing 60 mesh sieve), put in extraction kettle, regulating system temperature is to design temperature, regulation of carbon dioxide flow, to maximum, then keeps constant voltage to carry out cycling extraction.
Extraction yield=must oil mass/yellow elm powder of seeds amount × 100%
Orthogonal test condition: the consideration of synthesise various, according to the result of preliminary experiment, slective extraction pressure (A), temperature (B), time (C) are investigation factor, and each factor is got three levels, yellow elm seed oil extraction yield, for investigating index, is selected L9 (3
4) orthogonal table carries out orthogonal design, after static extracting 30min, keeps carbon dioxide flow velocity 3.0L/min, outlet temperature is 50 DEG C.
Table 8 factor level table
3 results and discussion
Table 9L
9(3
4) Orthogonal experiment results
Table 10 extracts yield analysis of variance table
F0.01(2，2)＝99，F0.05(2，2)＝19。(* * has utmost point significant, and * has conspicuousness).
Known according to the investigation result of supercritical extract yield, taking supercritical extract yield as index, there is extreme difference (R) A > B > C, can find out that by intuitive analysis the process conditions of final the best are A
3b
3c
3, and the best factor level being drawn by experiment is combined as A
1b
1c
2, therefore need to do confirmatory experiment.Show that through experimental verification optimum combination is A
3b
3c3, its extraction yield is the highest, i.e. pressure 40MPa, temperature 50 C, time 90min, yield is 5.032%.
Yellow elm seed oil its acid value, peroxide value, insoluble impurities, the moisture of supercritical carbon dioxide extracting and volatile substance content all meets GB GB2716 " edible vegetable oil sanitary standard " and GB2760 " food additives use sanitary standard " is better than yellow elm seed oil prepared by the method such as cold press, solvent extraction from appearance luster.
Detailed description of the invention
Following embodiment is for illustrating the preparation of antifatigue ready-mixed oil of the present invention, but it can not form any restriction to scope of the present invention, the difference of the ready-mixed oil that the oily yellow elm seed oil obtaining with cold-press of yellow elm seed simultaneously obtaining for proved invention supercritical carbon dioxide extracting is made, the yellow elm seed oil that uses respectively supercritical carbon dioxide extracting to obtain with two parts of method preparations in each embodiment and the yellow elm seed oil that cold-press obtains, mark and contrast respectively at aspects such as color and luster, mouthfeel, smells.
Embodiment 1
Major-minor oil plant proportioning:
10 kilograms of yellow elm seed oil, 2 kilograms of corn oils;
Preparation method:
By two kinds of oily mix and blends 30 minutes and get final product;
Quality evaluation:
| Yellow elm seed oil source | Color and luster | Mouthfeel | Smell |
| Supercritical carbon dioxide extracting | 9 | 8 | 8 |
| Cold-press | 8 | 7 | 6 |
Embodiment 2
Major-minor oil plant proportioning:
10 kilograms of yellow elm seed oil, 10 kilograms of corn oils;
Preparation method:
By two kinds of oily mix and blends 30 minutes and get final product;
Quality evaluation:
| Yellow elm seed oil source | Color and luster | Mouthfeel | Smell |
| Supercritical carbon dioxide extracting | 8 | 8 | 8 |
| Cold-press | 7 | 7 | 7 |
Embodiment 3
Major-minor oil plant proportioning:
10 kilograms of yellow elm seed oil, 30 kilograms of corn oils;
Preparation method:
By two kinds of oily mix and blends 30 minutes and get final product;
Quality evaluation:
| Yellow elm seed oil source | Color and luster | Mouthfeel | Smell |
| Supercritical carbon dioxide extracting | 8 | 9 | 8 |
| Cold-press | 8 | 7 | 8 |
Embodiment 4
Major-minor oil plant proportioning:
10 kilograms of yellow elm seed oil, 50 kilograms of corn oils;
Preparation method:
By two kinds of oily mix and blends 30 minutes and get final product;
Quality evaluation:
| Yellow elm seed oil source | Color and luster | Mouthfeel | Smell |
| Supercritical carbon dioxide extracting | 9 | 9 | 8 |
| Cold-press | 8 | 7 | 8 |
Embodiment 5
Major-minor oil plant proportioning:
10 kilograms of yellow elm seed oil, 80 kilograms of corn oils;
Preparation method:
By two kinds of oily mix and blends 30 minutes and get final product;
Quality evaluation:
| Yellow elm seed oil source | Color and luster | Mouthfeel | Smell |
| Supercritical carbon dioxide extracting | 10 | 10 | 10 |
| Cold-press | 9 | 9 | 9 |
Embodiment 6
Major-minor oil plant proportioning:
10 kilograms of yellow elm seed oil, 100 kilograms of corn oils;
Preparation method:
By two kinds of oily mix and blends 30 minutes and get final product;
Quality evaluation:
| Yellow elm seed oil source | Color and luster | Mouthfeel | Smell |
| Supercritical carbon dioxide extracting | 10 | 9 | 9 |
| Cold-press | 9 | 8 | 8 |
Claims (8)
1. the application of yellow elm seed oil in preparation antifatigue ready-mixed oil.
2. application as claimed in claim 1, is characterized in that described yellow elm seed oil is made up through carbon dioxide supercritical extraction of yellow elm seed.
3. application as claimed in claim 2, is characterized in that described carbon dioxide supercritical extraction condition is: pressure 20-40Mpa, temperature are that 30-50 DEG C, extraction time are 30-90 minute.
4. application as claimed in claim 3, is characterized in that described carbon dioxide supercritical extraction condition is: pressure 30Mpa, temperature are that 40 DEG C, extraction time are 90 minutes.
5. application as claimed in claim 1, the main oil plant that it is characterized in that described antifatigue ready-mixed oil is yellow elm seed oil, auxiliary oil plant is one or more in peanut oil, soybean oil, olive oil, rapeseed oil or corn oil.
6. application as claimed in claim 5, the main oil plant that it is characterized in that described antifatigue ready-mixed oil is yellow elm seed oil, auxiliary oil plant is corn oil.
7. application as claimed in claim 6, the main oil plant that it is characterized in that described antifatigue ready-mixed oil is yellow elm seed oil, and auxiliary oil plant is corn oil, and the two ratio is 1: 1-1: 10.
8. application as claimed in claim 1, is characterized in that the application of described yellow elm seed oil in the anti-oxidant ready-mixed oil of preparation.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114532414A (en) * | 2022-03-07 | 2022-05-27 | 大理大学 | Antioxidant edible walnut blend oil and preparation method thereof |
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