CN103908476B - Cordyceps militaris extract as well as preparation method and preparation thereof - Google Patents
Cordyceps militaris extract as well as preparation method and preparation thereof Download PDFInfo
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Abstract
The invention relates to the technical field of extraction of effective components and particularly relates to a cordyceps militaris extract as well as a preparation method and a preparation thereof. In the cordyceps militaris extract, the content of cordyceps polysaccharide is not less than 1700mg/L and the content of cordyceps adenosine is not less than 35mg/L. The invention further provides a preparation method of the extract and a cordyceps militaris preparation prepared by the extract. Experiments verify that compared with the cordyceps militaris extract extracted by a known negative pressure gas explosion assistant extraction method with a good extraction effect, the extraction rate of cordyceps polysaccharide by the method is improved by 9.19% while the extraction rate of cordyceps adenosine is improved by 7.17%. The contents of cordyceps polysaccharide and cordyceps adenosine in the cordyceps militaris extract extracted by the method provided by the invention are remarkably (p is less than 0.05) higher than those in the cordyceps militaris extract extracted by the negative pressure gas explosion assistant extraction method.
Description
Technical field
The present invention relates to extracts active ingredients technical field, more particularly, to a kind of Cordyceps militaris (L.) Link. extract and preparation method thereof with
Preparation.
Background technology
Cordyceps militaris (L.) Link. (cordyceps militaris) also known as Cordyceps militaris (L.) Link., Cordyceps militaris etc., are Ascomycotina, Ergota
Zoopagales, Clavicipitaceae, the type sepecies of Cordyceps, widely distributed in the world, it is a kind of famous and precious Chinese medicine and high tonic.Its
Active component and function are extremely similar to famous medicinal fungi Cordyceps, containing several amino acids, vitamin, micro unit
Element, sod, Cordycepses adenosine, cordycepic acid, the bioactive substance such as Cordyceps polysaccharide, and also the content of the material such as Cordycepses adenosine, nucleoside is also
Higher than Cordyceps, there is enhancing immunity, endocrine regulation, resisting fatigue, antioxidation, defying age, antibacterial, antitumor, anti-lack
Oxygen, calmness, blood sugar lowering, the protection different physiological roles such as Liver and kidney and respiratory system.
Wherein, Cordyceps polysaccharide is by mannose, cordycepin, adenosine, galactose, arabinose, xylose essence, glucose, rock
The polysaccharide of algae sugar composition.It is demonstrated experimentally that Cordyceps polysaccharide can improve immune function of human body, leukocyte increasing, clinic has been used for controlling
Treat malignant tumor.In addition Cordyceps are additionally operable to control pulmonary tuberculosis, shortness of breath is breathed with cough, sexual impotence, nocturnal emission, spontaneous sweating, soreness of waist and knee joint, with
When have hypoglycemic effect.Cordycepses adenosine mainly includes uracil, adenine, adenine, hypoxanthine, guanine, adenine
Deng having antitumor action.
At present, the extracting method directly carrying out active substance to Cordyceps militaris (L.) Link. itself there are still that extraction efficiency is low, extracted
Journey destroys serious problem to active substance.Therefore, for the exploitation of Cordyceps militaris (L.) Link., it is with fermentation mode or in fermentation base mostly
Active substance extraction is carried out on plinth carry out.However, the increased periods extraction time of fermentation, reduce the efficiency of extraction.Therefore,
Develop a kind of new Cordyceps militaris (L.) Link. active substance extracting method, improve extraction efficiency, reduce the destruction to active substance for the extraction process,
And to shorten extraction time be very necessary.
Content of the invention
In view of this, the technical problem to be solved in the present invention be to provide a kind of Cordyceps militaris (L.) Link. extract and preparation method thereof with
Preparation.In the Cordyceps militaris (L.) Link. extract that the present invention provides, the content of Cordyceps polysaccharide and Cordycepses adenosine is higher, and this preparation method can
Fast and effectively improve active component in Cordyceps militaris (L.) Link., reduce the destruction to active substance for the extraction process.
The invention provides a kind of Cordyceps militaris (L.) Link. extract, the content of wherein Cordyceps polysaccharide is not less than 1700mg/l, Cordycepses gland
The content of glycosides is not less than 35mg/l.
In prior art, improper due to extracting method, reduce to active substance extraction efficiency in Cordyceps militaris (L.) Link., generally,
Cordyceps polysaccharide content in Cordyceps militaris (L.) Link. extract is less than 1620mg/l, and the content of Cordycepses adenosine is also less than 34mg/l.
The preparation method of the Cordyceps militaris (L.) Link. extract that the present invention provides, comprises the following steps:
Step 1: the Cordyceps militaris (L.) Link. that water content is 20%～90% sprays quick-fried, product in the middle of prepared first through supercritical carbon dioxide
Thing；
Step 2: by the first intermediate product with water with mass ratio as 1:5～15 mix, be crushed to particle diameter as 0.005mm～
0.02mm, adjusting ph value is 5～8, and temperature is 18 DEG C～30 DEG C, stirs 0.5h～2h, through centrifugation, must precipitate and liquid, collects
Liquid is the second intermediate product；
Step 3: will precipitate with water with mass ratio as 1:5～15 mix, adjusting ph value is 5～8, and temperature is 18 DEG C～30
DEG C, stir 0.5h～2h, after precipitation is abandoned in centrifugation, mix with the second intermediate product, concentrate, obtain final product.
Preferably, supercritical carbon dioxide spray quick-fried particularly as follows: by water content be 20%～90% Cordyceps militaris (L.) Link. load pressure
In container, into pressure vessel, injection food stage liquid carbon dioxide to container inner pressure reaches 70mpa～100mpa and liquid two
Carbonoxide can be totally submerged Cordycepses, opens relief valve blood pressure lowering spray quick-fried after keeping 1h～5h.
The present invention adopts supercritical carbon dioxide to spray quick-fried technology, and its principle is mainly Cordyceps militaris (L.) Link. and is dropped suddenly by high pressure in moment
To normal pressure, the carbon dioxide within raw material gasifies suddenly, expands, and occurs spray quick-fried, volume increases more than 3 times, so that Cordyceps militaris (L.) Link.
In spongy, volume increase, some structure organizations such as fibre bundle etc. is destroyed, and inclusions expose, and are conducive to for histiocyte rupture
Cordyceps polysaccharide, the dissolution of Cordycepses adenosine, improve the extraction yield of functional mass.And, in technical scheme, through two
Carbonoxide spray is quick-fried process after Cordyceps militaris (L.) Link. extracted using 18 DEG C～30 DEG C of room temperature during extracting, it is to avoid tradition
Technique high temperature extracts the destruction to active substance in Cordyceps militaris (L.) Link., improves wherein Cordyceps polysaccharide, the extraction efficiency of Cordycepses adenosine.
It is as fast as possible for the upper limit that the quick-fried speed of pressure release spray does not form drikold with container.
Preferably, the granularity that water content is 20%～90% Cordyceps militaris (L.) Link. is 2 mesh～40 mesh.
Present invention research shows, in the carbon dioxide spray of the present invention is quick-fried, affects titanium dioxide when the granularity of Cordyceps militaris (L.) Link. is excessive
The effect that the infiltration of carbon is immersed and spray is quick-fried, is also unfavorable for the preparation in later stage simultaneously；And granularity be less than 40 mesh when, material be in fine powder
Shape, single volume of material expansion effect is poor, is not suitable for carrying out spraying quick-fried process.
Preferably, the water content of Cordyceps militaris (L.) Link. is 50%～90%.
Present invention research shows, in the carbon dioxide spray of the present invention is quick-fried, the too low Cordyceps militaris (L.) Link. of water content lacks flexibility and exists
Frangible in pressure process, and when moisture is too high, it is unfavorable for the immersion of carbon dioxide, the shell-broken effect of impact Cordyceps militaris (L.) Link..
Preferably, the preparation method of the Cordyceps militaris (L.) Link. of water content 20%～90% is: from the infested Cordyceps militaris (L.) Link. that no goes mouldy,
After cleaning remove impurity, round a Cordyceps militaris (L.) Link. or the Cordyceps militaris (L.) Link. through cutting absorbs water to water content for 20%～90%, seal all after dewatering
Wet.
Preferably, water suction, particularly as follows: rounding a Cordyceps militaris (L.) Link. or the Cordyceps militaris (L.) Link. through cutting, is soaked or spray water with water.
Preferably, spring or winter, the time of water suction is 30min～60min, and the time of sam is 40～90min；Summer
Or autumn, absorbent time is 20min～40min, and the sam time is 30min～60min.
Preferably, being crushed to particle diameter in step 2 is 0.008mm～0.01mm.
Preferably, concentrating the quality of Cordyceps militaris (L.) Link. and the Cordyceps militaris (L.) Link. extract making that water content is 20%～90% in step 3
Volume ratio be 1g:15ml.
Present invention also offers a kind of Cordyceps militaris (L.) Link. preparation, the Cordyceps militaris (L.) Link. extract providing including the present invention.
The Cordyceps militaris (L.) Link. that in the Cordyceps militaris (L.) Link. extraction that the present invention provides, the content of active substance is extracted apparently higher than prior art carries
Take thing, prepared and remain to keep higher activity substance content after being referred to as Cordyceps militaris (L.) Link. preparation, thus reaching more preferable health care effect
Really.
Preferably, the Cordyceps militaris (L.) Link. preparation that the present invention provides also includes acceptable adjuvant on pharmacy or food.
Preferably, on pharmacy or food, acceptable adjuvant is sweeting agent, acidic flavoring agent, stabilizer or Sal.
It is furthermore preferred that sweeting agent is sucrose；Acidic flavoring agent is citric acid, and stabilizer is pectin or xanthan gum.
Preferably, the dosage form of the Cordyceps militaris (L.) Link. preparation of present invention offer is oral solutionses.
The preparation method of Cordyceps militaris (L.) Link. preparation that the present invention provides is: take the Cordyceps militaris (L.) Link. extract that the present invention provides through homogenizing,
Sterilization, obtains final product.
Preferably, the pressure of homogenizing is 20mpa～50mpa, number of times is 1 time～2 times.
Preferably, the temperature of sterilization is 105 DEG C～110 DEG C, the time is 30min.
The invention provides a kind of Cordyceps militaris (L.) Link. extract, the content of wherein Cordyceps polysaccharide is not less than 1700mg/l, Cordycepses gland
The content of glycosides is not less than 35mg/l.Present invention also offers the preparation method of this extract, and the pupa worm prepared by this extract
Careless preparation.In the Cordyceps militaris (L.) Link. extraction that the present invention provides, the content of active substance is extracted apparently higher than the Cordyceps militaris (L.) Link. that prior art is extracted
Thing.And this preparation method realize in the case of non-heated spray quick-fried, and adopt the non-thermal water extraction of room temperature.Improve Cordyceps polysaccharide and worm
The yield of careless adenosine.The present invention experiments prove that, extracts with generally acknowledged extraction effect preferable negative pressure air blasting assisted extraction method
Cordyceps militaris (L.) Link. extract compare, the method that the present invention provides improves 9.19% to the extraction ratio of Cordyceps polysaccharide, and to Cordycepses gland
The extraction ratio of glycosides improves 7.17%；And with Cordyceps polysaccharide and Cordycepses in the Cordyceps militaris (L.) Link. extract of the method extraction of present invention offer
The content of adenosine notable (p ＜ 0.05) is higher than the Cordyceps militaris (L.) Link. extract being extracted with negative pressure air blasting assisted extraction method.
Specific embodiment
The invention provides a kind of Cordyceps militaris (L.) Link. extract and preparation method thereof and preparation, those skilled in the art can use for reference
Present disclosure, is suitably modified technological parameter and realizes.Specifically, all similar replacements and change are to this area skill
For art personnel it is it will be apparent that they are considered as including in the present invention.The method of the present invention and application have been passed through relatively
Good embodiment is described, and related personnel substantially can be to methods herein in without departing from present invention, spirit and scope
It is modified with application or suitably changes and combine, to realize and to apply the technology of the present invention.
The instrument that the present invention adopts is all common commercially available product, all can buy in market.
With reference to embodiment, the present invention be expanded on further:
The method that embodiment 1 is provided with the present invention prepares Cordyceps militaris (L.) Link. extract
From the infested Cordyceps militaris (L.) Link. that no goes mouldy, after cleaning remove impurity, weigh 1kg Cordyceps militaris (L.) Link., Cordyceps militaris (L.) Link. is improved using infusion method
To 80%, sealing 50min sam after dewatering makes its internal moisture equalize to water content, is then placed in pressure vessel, to resistance to pressure-volume
In device, injection food-class liquid CO 2 to container inner pressure reaches 81mpa and liquid CO 2 can be totally submerged Cordycepses, protects
Open relief valve blood pressure lowering spray after holding 4h quick-fried, first intermediate product is obtained.
First intermediate product is added water making beating by solid-liquid ratio 1: 15, crossing colloid mill and being crushed to particle diameter is 0.008mm, often
Temperature does not heat, and it is 5.5 that hydrochloric acid adjusts ph value, and 1h is extracted in stirring.Obtain precipitation and the second intermediate product through centrifugation.Precipitation presses solid-liquid ratio
1: 10 room temperature does not heat, and it is 5.5 that hydrochloric acid adjusts ph value, and 1h is extracted in stirring, after precipitation is abandoned in centrifugation, mixes with the second intermediate product, dense
Contracting is settled to 15l, obtains final product.The method that embodiment 2 is provided with the present invention prepares Cordyceps militaris (L.) Link. extract
From the infested Cordyceps militaris (L.) Link. that no goes mouldy, after cleaning remove impurity, weigh 1kg Cordyceps militaris (L.) Link., Cordyceps militaris (L.) Link. is improved using infusion method
To 20%, sealing 90min sam after dewatering makes its internal moisture equalize to water content, is then placed in pressure vessel, to resistance to pressure-volume
In device, injection food-class liquid CO 2 to container inner pressure reaches 70mpa and liquid CO 2 can be totally submerged Cordycepses, protects
Open relief valve blood pressure lowering spray after holding 1h quick-fried, first intermediate product is obtained.
First intermediate product is added water making beating by solid-liquid ratio 1: 10, crossing particle diameter that colloid mill is crushed to is 0.005mm,
Room temperature does not heat, and it is 5 that hydrochloric acid adjusts ph value, and 0.5h is extracted in stirring.Obtain precipitation and the second intermediate product through centrifugation.Precipitation presses feed liquid
Do not heat than 1: 5 room temperature, it is 5 that hydrochloric acid adjusts ph value, 0.5h is extracted in stirring, after precipitation is abandoned in centrifugation, mixes with the second intermediate product, dense
Contracting is settled to 15l, obtains final product.The method that embodiment 3 is provided with the present invention prepares Cordyceps militaris (L.) Link. extract
Gone mouldy infested Cordyceps militaris (L.) Link. from nothing, be cut into after cleaning remove impurity length be 1cm about Cordyceps militaris (L.) Link. section, weigh 1kg
Cordyceps militaris (L.) Link. section, using spraying raising Cordyceps militaris (L.) Link. water content to 90%, sealing 30min sam after dewatering makes its internal moisture equal
Weighing apparatus, is then placed in pressure vessel, and into pressure vessel, injection food-class liquid CO 2 reaches 100mpa to container inner pressure
And liquid CO 2 can be totally submerged Cordycepses, open relief valve blood pressure lowering after keeping 5h and spray quick-fried, prepared first intermediate product.
First intermediate product is added water making beating by solid-liquid ratio 1: 8, crossing colloid mill and being crushed to particle diameter is 0.02mm, room temperature
Do not heat, it is 8 that hydrochloric acid adjusts ph value, and 2h is extracted in stirring.Through 200 mesh filter cloth suction filterings, obtain precipitation and the second intermediate product.Precipitation is pressed
Solid-liquid ratio 1: 8 room temperature does not heat, and it is 8 that hydrochloric acid adjusts ph value, and 2h is extracted in stirring, abandons after precipitation through 200 mesh filter cloth suction filterings, in second
Between product mixing, concentrate be settled to 15l, obtain final product.
The method that embodiment 4 is provided with the present invention prepares Cordyceps militaris (L.) Link. extract
Gone mouldy infested Cordyceps militaris (L.) Link. from nothing, be cut into after cleaning remove impurity length be 1cm about Cordyceps militaris (L.) Link. section, weigh 1kg
Cordyceps militaris (L.) Link. section, using spraying raising Cordyceps militaris (L.) Link. water content to 50%, sealing 50min sam after dewatering makes its internal moisture equal
Weighing apparatus, be then placed in pressure vessel, into pressure vessel injection food-class liquid CO 2 to container inner pressure reach 90mpa and
Liquid CO 2 can be totally submerged Cordycepses, open relief valve blood pressure lowering and spray quick-fried, prepared first intermediate product after keeping 3h.
First intermediate product is added water making beating by solid-liquid ratio 1: 10, crossing colloid mill and being crushed to particle diameter is 0.01mm, often
Temperature does not heat, and it is 6.5 that hydrochloric acid adjusts ph value, and 1.2h is extracted in stirring.Through 200 mesh filter cloth suction filterings, obtain precipitation and the second intermediate product.
Precipitation is not heated by solid-liquid ratio 1: 9 room temperature, and it is 6.5 that hydrochloric acid adjusts ph value, and 1.2h is extracted in stirring, abandons precipitation through 200 mesh filter cloth suction filterings
Afterwards, mix with the second intermediate product, concentrate and be settled to 15l, obtain final product.
Comparative example 1 prepares Cordyceps militaris (L.) Link. extract with negative pressure air blasting assisted extraction method
From the infested Cordyceps militaris (L.) Link. that no goes mouldy, after cleaning remove impurity, weigh 1kg Cordycepses, place into the swollen of negative pressure bulking equipment
Change in tank, Bulking tank is heated to 80 DEG C of holding 30min, and in vacuum tank, vacuum is evacuated to opens solenoid valve wink during below -0.09mpa
Between pressure release air blasting, be obtained the first intermediate product.
First intermediate product is added water making beating by solid-liquid ratio 1: 15, crossing colloid mill and being crushed to particle diameter is 0.008mm, plus
To 90 DEG C, it is 5.5 that hydrochloric acid adjusts ph value to heat, and 1h is extracted in stirring.Obtain precipitation and the second intermediate product through centrifugation.Precipitation presses solid-liquid ratio
1: 10, it is heated to 90 DEG C, it is 5.5 that hydrochloric acid adjusts ph value, 1h is extracted in stirring, after precipitation is abandoned in centrifugation, mixes with the second intermediate product, dense
Contracting is settled to 15l, obtains final product.
Comparative example 2 prepares Cordyceps militaris (L.) Link. extract with negative pressure air blasting assisted extraction method
Gone mouldy infested Cordyceps militaris (L.) Link. from nothing, be cut into after cleaning remove impurity length be 1cm about Cordyceps militaris (L.) Link. section, weigh 1kg
Cordyceps militaris (L.) Link. section, places in the Bulking tank of negative pressure bulking equipment, and Bulking tank is heated to 90 DEG C of holding 30min, vacuum in vacuum tank
Degree is evacuated to opens solenoid valve moment pressure release air blasting during below -0.09mpa, and first intermediate product is obtained.
First intermediate product is added water making beating by solid-liquid ratio 1: 10, crossing colloid mill and being crushed to particle diameter is 0.01mm, plus
To 92 DEG C, it is 6.5 that hydrochloric acid adjusts ph value to heat, and 1.2h is extracted in stirring.Through 200 mesh filter cloth suction filterings, obtain precipitation and the second intermediate product.
Precipitation presses solid-liquid ratio 1: 9, is heated to 92 DEG C, and it is 6.5 that hydrochloric acid adjusts ph value, and 1.2h is extracted in stirring, abandons precipitation through 200 mesh filter cloth suction filterings
Afterwards, mix with the second intermediate product, concentrate and be settled to 15l, obtain final product.
The Cordyceps militaris (L.) Link. extract quality of embodiment 5 present invention and prior art preparation compares
Phenol sulfuric acid procedure and high performance liquid chromatography is adopted to detect and calculate the method for present invention offer and with negative pressure air blasting
Cordyceps polysaccharide in the Cordyceps militaris (L.) Link. extract of assisted extraction method preparation and the content of Cordycepses adenosine, every group is done 3 groups of repetitions, result
As shown in table 1:
The Cordyceps militaris (L.) Link. extract quality that table 1 present invention is prepared with prior art compares
Note: the alphabetical difference person's significant difference (p < 0.05) of colleague.
As can be seen from Table 1, the technology of the present invention and existing more advanced negative pressure puffing technique are in Cordyceps militaris (L.) Link. extraction system
Significant difference (p < 0.05) on main extraction index polysaccharide in standby oral liquid and adenosine, it is swollen that the technology of the present invention is substantially better than negative pressure
Change matched group.
The preparation of embodiment 6 Cordyceps militaris (L.) Link. oral liquid
The Cordyceps militaris (L.) Link. extract of any one of Example 1～4 preparation, homogenizing 2 times under 35mpa pressure.Use after homogenizing
25ml bottle fill, using 110 DEG C of sterilization 30min, as original flavor Cordyceps militaris (L.) Link. is administered orally liquid product.
The preparation of embodiment 7 Cordyceps militaris (L.) Link. oral liquid
The Cordyceps militaris (L.) Link. extract of any one of Example 1～4 preparation, homogenizing 1 time under 40mpa pressure.Use after homogenizing
10ml bottle fill, using 105 DEG C of sterilization 30min, as original flavor Cordyceps militaris (L.) Link. is administered orally liquid product.Embodiment 8 Cordyceps militaris (L.) Link. oral liquid
Preparation
The Cordyceps militaris (L.) Link. extract of any one of Example 1～4 preparation, adds sucrose, citric acid and pectin to be seasoned,
Homogenizing 1 time under 40mpa pressure.10ml bottle fill is used, using 105 DEG C of sterilization 30min, as Cordyceps militaris (L.) Link. oral liquid produces after homogenizing
Product.
The above is only the preferred embodiment of the present invention it is noted that coming for those skilled in the art
Say, under the premise without departing from the principles of the invention, some improvements and modifications can also be made, these improvements and modifications also should be regarded as
Protection scope of the present invention.
Claims (10)
1. a kind of Cordyceps militaris (L.) Link. extract is it is characterised in that the content of wherein Cordyceps polysaccharide is not less than 1700mg/l, Cordycepses adenosine
Content is not less than 35mg/l；
Its preparation method comprises the following steps:
Step 1: the Cordyceps militaris (L.) Link. that water content is 20%～90% is mixed with liquid carbon dioxide, pressure 70mpa～100mpa protects
After holding 1h～5h, pressure release spray is quick-fried, and first intermediate product is obtained；
Step 2: by described first intermediate product with water with mass ratio as 1:5～15 mix, be crushed to particle diameter as 0.005mm～
0.02mm, adjusting ph value is 5～8, and temperature is 18 DEG C～30 DEG C, stirs 0.5h～2h, through solid-liquid separation, must precipitate and liquid,
Collecting described liquid is the second intermediate product；
Step 3: by described precipitation with water with mass ratio as 1:5～15 mix, regulation ph value is 5～8, and temperature is 18 DEG C～30
DEG C, stir 0.5h～2h, after precipitation is abandoned in centrifugation, mix with the second intermediate product, concentrate, obtain final product.
2. the preparation method of Cordyceps militaris (L.) Link. extract described in claim 1 is it is characterised in that comprise the following steps:
Step 1: the Cordyceps militaris (L.) Link. that water content is 20%～90% is mixed with liquid carbon dioxide, pressure 70mpa～100mpa protects
After holding 1h～5h, pressure release spray is quick-fried, and first intermediate product is obtained；
Step 2: by described first intermediate product with water with mass ratio as 1:5～15 mix, be crushed to particle diameter as 0.005mm～
0.02mm, adjusting ph value is 5～8, and temperature is 18 DEG C～30 DEG C, stirs 0.5h～2h, through solid-liquid separation, must precipitate and liquid,
Collecting described liquid is the second intermediate product；
Step 3: by described precipitation with water with mass ratio as 1:5～15 mix, regulation ph value is 5～8, and temperature is 18 DEG C～30
DEG C, stir 0.5h～2h, after precipitation is abandoned in centrifugation, mix with the second intermediate product, concentrate, obtain final product.
3. preparation method according to claim 2 is it is characterised in that described water content is 20%～90% Cordyceps militaris (L.) Link.
Granularity is 2 mesh～40 mesh.
4. preparation method according to claim 2 is it is characterised in that the water content of described Cordyceps militaris (L.) Link. is 50%～90%.
5. preparation method according to claim 2 it is characterised in that be crushed to described in step 2 particle diameter be 0.008mm～
0.01mm.
6. preparation method according to claim 2 makes the described water content be it is characterised in that concentrating described in step 3
The quality of 20%～90% Cordyceps militaris (L.) Link. is 1g:15ml with the ratio of the volume of described Cordyceps militaris (L.) Link. extract.
7. a kind of Cordyceps militaris (L.) Link. preparation is it is characterised in that include Cordyceps militaris (L.) Link. extract as claimed in claim 1.
8. Cordyceps militaris (L.) Link. preparation according to claim 7 it is characterised in that its also include acceptable auxiliary on pharmacy or food
Material.
9. Cordyceps militaris (L.) Link. preparation according to claim 7 is it is characterised in that its dosage form is oral solutionses.
10. the preparation method of the Cordyceps militaris (L.) Link. preparation as described in any one of claim 7～9 is it is characterised in that will be as claim
Cordyceps militaris (L.) Link. extract described in 1, through homogenizing, sterilization, obtains final product.
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| CN103421655A (en) * | 2013-08-17 | 2013-12-04 | 刘润成 | Cordyceps militaris juice wine and production method thereof |
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| CN101007025A (en) * | 2006-01-24 | 2007-08-01 | 上海市农业科学院 | A continuous extraction method of effective ingredients from fruitbodies of Cordyceps militaris |
| CN101690463A (en) * | 2009-08-21 | 2010-04-07 | 吉林大学 | Mutagenic strain of cordyceps militaris and breeding method |
| CN101717709A (en) * | 2009-12-24 | 2010-06-02 | 孙国明 | Preparation method of cordyceps wine |
| CN102726216A (en) * | 2012-07-17 | 2012-10-17 | 杨毅 | Method for culturing cordyceps militaris anti-degradation strain |
| CN103421655A (en) * | 2013-08-17 | 2013-12-04 | 刘润成 | Cordyceps militaris juice wine and production method thereof |
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