CN105341927A - Preparation method of cordyceps militaris and vitis vinifera ferment having cosmetic effect - Google Patents

Preparation method of cordyceps militaris and vitis vinifera ferment having cosmetic effect Download PDF

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Publication number
CN105341927A
CN105341927A CN201510865364.0A CN201510865364A CN105341927A CN 105341927 A CN105341927 A CN 105341927A CN 201510865364 A CN201510865364 A CN 201510865364A CN 105341927 A CN105341927 A CN 105341927A
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ferment
cordyceps militaris
liquid
extract
temperature
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CN201510865364.0A
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不公告发明人
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Shenyang Yuankang Natural Biological Technology Co Ltd
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Shenyang Yuankang Natural Biological Technology Co Ltd
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Priority to CN201510865364.0A priority Critical patent/CN105341927A/en
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES
    • A23V2002/00Food compositions, function of food ingredients or processes for food or foodstuffs

Abstract

The invention discloses a preparation method of a cordyceps militaris and vitis vinifera ferment having a cosmetic effect. The method comprises the specific steps of: step I, preparing a yeast strain solution; step II, preparing a lactic acid bacteria solution, step III, performing fermentation culture; step IV, performing centrifugation treatment so as to obtain a raw ferment solution; and step V, adding a botanical extract to the raw ferment solution. According to the preparation method disclosed by the invention, the characteristics of the ferment and the characteristics of cordyceps militaris are combined, the ferment and the cordyceps militaris are in mixed fermentation or compounding, and then, other active components are added for compounding, so that the cordyceps militaris and ferment products can be developed in an innovative manner. The cordyceps militaris and vitis vinifera ferment integrates the efficacies of the ferment and the cordyceps militaris, and has the efficacies of optimizing the metabolic process, beautifying the features, improving the skin elasticity, reducing erythema in skins, reducing wrinkles in skins, diminishing the pores and the like.

Description

A kind of Cordyceps militaris grape ferment preparation method having cosmetic result
Technical field
The present invention relates to bio-science field, be specifically related to a kind of Cordyceps militaris grape ferment preparation method having cosmetic result.
Background technology
Ferment is also called " enzyme ", is the polymer substance with biocatalytic Activity.Nearly all cellular activity process all needs the participation of ferment.Modern society, along with the quickening of people's rhythm of life and the aggravation of environmental pollution, the content of people's body endo enzyme reduces day by day, and then reduces body immunity and metabolic rate, and the probability making people ill increases.At present, the ferment content in daily bread is too low, cannot meet the normal need of people.In order to meet modern to diversity, the balance of nutritional need be easy to absorbability, the production of efficient ferment product is extremely urgent.
Cordyceps militaris has the active component similar with Cordyceps sinensis and pharmacological action, is called as " Cordyceps sinensis " that can manually cultivate.2009, Cordyceps militaris was approved for new resource food, and its price is far below Cordyceps sinensis, was thus more and more subject to the favor of consumers in general.The traditional Chinese medical science is thought, Cordyceps militaris enters lung kidney two warp, and both tonifying lung was cloudy, and kidney-replenishing again, function cures mainly as invigorating the lung and the kidney, relieving cough and reducing sputum.Modern medicine study its there is cellular immunity and Humoral Immunological Regulation Roles, also there is antifatigue effect.Therefore, be the product of primary raw material if can develop with Cordyceps militaris, further developing of Cordyceps militaris industry can not only be promoted, more can bring happiness for the health of broad masses of the people.Although the product that on market, existing Cordyceps militaris is relevant, market scale is little, and added value is low, and effect is indefinite, needs badly and develops cordyceps militaris series products that is efficient, high added value.
Summary of the invention
The object of the invention is to, a kind of Cordyceps militaris grape ferment preparation method having cosmetic result is provided, with
Customer service is to existing method Problems existing.
The object of the invention is to be achieved through the following technical solutions: a kind of Cordyceps militaris grape ferment preparation method having cosmetic result, described in this, method comprises:
Step one: prepared by barms liquid, plant flow container and expand culture medium, YPD fluid nutrient medium, its mass percent is 1% yeast extract, 2% peptone, 2% glucose, 2% agar, and YPD fluid nutrient medium is put into 0.5M by 93% water 3seeding tank in, 115 DEG C, 30-50 minute high-temperature sterilization; Under aseptic technique, 1.5 kilograms of saccharomycete dry bacterium powders are dissolved in the sterilized water of 3 times, form bacterium liquid, described bacterium liquid is loaded serum bottle and is inoculated into 0.5M 3seeding tank in, at 28-30 DEG C, pass into aseptic compressed air, throughput is 0.2-0.3M 3/ min, cultivates 20-30h, until thalli growth to stopping during logarithmic phase latter stage cultivating, repeats the kind liquid that above-mentioned steps can obtain the yeast liquid of required quality.
Step 2: prepared by lactobacillus inoculation liquid, plants flow container and expands culture medium: enrichment medium of wort, its mass percent is: 10% brewer's wort, 0.5% soy peptone, 0.5% yeast extract, 1% beef extract, 0.2%K 2hPO 4,87.8% water, puts into 0.5M by enrichment medium of wort 3in seeding tank, 1.5 kilograms of lactic acid bacteria dry bacterium powders are dissolved in the sterilized water of 3 times under aseptic technique by high-temperature sterilization 30-50min at 115 DEG C, and bacterium liquid loads serum bottle and is inoculated into 0.5M 3seeding tank, 37 DEG C of constant temperature quiescent culture 24-36h, until thalli growth to stopping during logarithmic phase latter stage cultivating, repeat the kind liquid that above-mentioned steps can obtain required lactic acid bacteria;
Step 3: fermented and cultured, be the grape of 110:50:40:1000 by mass ratio, pears, pakchoi, water mixing is smashed rear filtration and is obtained filtrate, culture medium is made with described filtrate, high-temperature sterilization 30-50 minute at 115 DEG C, described culture medium is added in fermentation tank, then be the saccharomycetic kind of liquid that the inoculum concentration inoculation step 1 of the 5%-10% of described filtrate obtains by quality, 48h is cultivated at the temperature of 28 DEG C, the volume of described fermentation tank is V cubic meter, throughput is (0.4-0.6) V cubic meters per minute, stir, then be the lactobacillus inoculation liquid that the inoculum concentration inoculation step 2 of the 5%-10% of described filtrate obtains by quality, continue to cultivate 5-7d to fermentation ends at the temperature of 37 DEG C, obtain zymotic fluid,
Step 4: centrifugal treating obtains ferment stoste, and zymotic fluid is imported centrifuge, and through the centrifugal 10min of 6000rpm, gained supernatant is ferment stoste;
Step 5: add plant extracts to ferment stoste, be that 20:0.15:14:21:1000 is mixed to join ferment stoste by aloe extract, sucrose, ginger extract, Cordyceps militaris extract, stoste according to mass ratio, 115 degree of 30-50 minute high-temperature sterilizations, carry out packing.
Above-mentioned a kind of Cordyceps militaris grape ferment preparation method having cosmetic result, described speed of agitator is 100-120rpm/min.
Above-mentioned a kind of Cordyceps militaris grape ferment preparation method having cosmetic result, described lactic acid bacteria is streptococcus thermophilus.
The invention has the advantages that: compared with prior art, both, in conjunction with the features of ferment and Cordyceps militaris, are carried out mixed culture fermentation or composite, then are equipped with other active component, develop Cordyceps militaris ferment product innovatively by the present invention.Effect of this product set ferment and Cordyceps militaris, have optimize metabolic process, beauty treatment, increase skin elasticity, reduce skin erythema, reduce skin wrinkle, reduce the effects such as pore.
Detailed description of the invention
Below in conjunction with specific embodiment, technical scheme of the present invention is described in detail, to understand object, the scheme of the present invention further, but not as the restriction of claims protection domain of the present invention.
Embodiment 1.The invention discloses a kind of Cordyceps militaris grape ferment preparation method having cosmetic result, the method
Comprise:
Step one: prepared by barms liquid, plant flow container and expand culture medium, YPD fluid nutrient medium, its mass percent is 1% yeast extract, 2% peptone, 2% glucose, 2% agar, and YPD fluid nutrient medium is put into 0.5M by 93% water 3seeding tank in, 115 DEG C, 30-50 minute high-temperature sterilization; Under aseptic technique, 1.5 kilograms of saccharomycete dry bacterium powders are dissolved in the sterilized water of 3 times, form bacterium liquid, described bacterium liquid is loaded serum bottle and is inoculated into 0.5M 3seeding tank in, at 28-30 DEG C, pass into aseptic compressed air, throughput is 0.2-0.3M 3/ min, cultivates 20-30h, until thalli growth to stopping during logarithmic phase latter stage cultivating, repeats the kind liquid that above-mentioned steps can obtain the yeast liquid of required quality.
Step 2: prepared by thermophilus bacterial classification liquid, plants flow container and expands culture medium: enrichment medium of wort, its mass percent is: 10% brewer's wort, 0.5% soy peptone, 0.5% yeast extract, 1% beef extract, 0.2%K 2hPO 4,87.8% water, puts into 0.5M by enrichment medium of wort 3in seeding tank, 1.5 kilograms of streptococcus thermophilus dry bacterium powders are dissolved in the sterilized water of 3 times under aseptic technique by high-temperature sterilization 30-50min at 115 DEG C, and bacterium liquid loads serum bottle and is inoculated into 0.5M 3seeding tank, 37 DEG C of constant temperature quiescent culture 24-36h, until thalli growth to stopping during logarithmic phase latter stage cultivating, repeat the kind liquid that above-mentioned steps can obtain required streptococcus thermophilus;
Step 3: fermented and cultured, be the grape of 110:50:40:1000 by mass ratio, pears, pakchoi, water mixing is smashed rear filtration and is obtained filtrate, culture medium is made with described filtrate, high-temperature sterilization 30-50 minute at 115 DEG C, described culture medium is added in fermentation tank, then be the saccharomycetic kind of liquid that the inoculum concentration inoculation step 1 of the 5%-10% of described filtrate obtains by quality, 48h is cultivated at the temperature of 28 DEG C, the volume of described fermentation tank is V cubic meter, throughput is (0.4-0.6) V cubic meters per minute, speed of agitator is 100-120rpm/min, then be the lactobacillus inoculation liquid that the inoculum concentration inoculation step 2 of the 5%-10% of described filtrate obtains by quality, continue to cultivate 5-7d to fermentation ends at the temperature of 37 DEG C, obtain zymotic fluid,
Step 4: centrifugal treating obtains ferment stoste, and zymotic fluid is imported centrifuge, and through the centrifugal 10min of 6000rpm, gained supernatant is ferment stoste;
Step 5: add plant extracts to ferment stoste, be that 20:0.15:14:21:1000 is mixed to join ferment stoste by aloe extract, sucrose, ginger extract, Cordyceps militaris extract, stoste according to mass ratio, 115 degree of 30-50 minute high-temperature sterilizations, carry out packing.
Embodiment 2.The evaluation and test volunteer participating in the Cordyceps militaris Kiwi berry ferment of cosmetic result has 19 people, and sooner or later respectively taking 1 time, each 100-200ml for each person every day, 15 days is an evaluation and test cycle.Compare before and after taking, the average color cellulose content decline 17-30% of 19 people, skin elasticity increases 21-31%, and moisture content of skin increases 8-13%, and pore dia reduces 13-23%, and erythema reduces 13-23%.
Above the detailed description of the invention of invention is described; but these explanations can not be understood to limit scope of the present invention; protection scope of the present invention is limited by the claims of enclosing, and any change on the claims in the present invention basis is all protection scope of the present invention.

Claims (3)

1. there is a Cordyceps militaris grape ferment preparation method for cosmetic result, it is characterized in that: described method comprises:
Step one: prepared by barms liquid, plant flow container and expand culture medium, YPD fluid nutrient medium, its mass percent is 1% yeast extract, 2% peptone, 2% glucose, 2% agar, and YPD fluid nutrient medium is put into 0.5M by 93% water 3seeding tank in, 115 DEG C, 30-50 minute high-temperature sterilization; Under aseptic technique, 1.5 kilograms of saccharomycete dry bacterium powders are dissolved in the sterilized water of 3 times, form bacterium liquid, described bacterium liquid is loaded serum bottle and is inoculated into 0.5M 3seeding tank in, at 28-30 DEG C, pass into aseptic compressed air, throughput is 0.2-0.3M 3/ min, cultivates 20-30h, until thalli growth to stopping during logarithmic phase latter stage cultivating, repeats the kind liquid that above-mentioned steps can obtain the yeast liquid of required quality;
Step 2: prepared by lactobacillus inoculation liquid, plants flow container and expands culture medium: enrichment medium of wort, its mass percent is: 10% brewer's wort, 0.5% soy peptone, 0.5% yeast extract, 1% beef extract, 0.2%K 2hPO 4,87.8% water, puts into 0.5M by enrichment medium of wort 3in seeding tank, 1.5 kilograms of lactic acid bacteria dry bacterium powders are dissolved in the sterilized water of 3 times under aseptic technique by high-temperature sterilization 30-50min at 115 DEG C, and bacterium liquid loads serum bottle and is inoculated into 0.5M 3seeding tank, 37 DEG C of constant temperature quiescent culture 24-36h, until thalli growth to stopping during logarithmic phase latter stage cultivating, repeat the kind liquid that above-mentioned steps can obtain required lactic acid bacteria;
Step 3: fermented and cultured, be the grape of 110:50:40:1000 by mass ratio, pears, pakchoi, water mixing is smashed rear filtration and is obtained filtrate, culture medium is made with described filtrate, high-temperature sterilization 30-50 minute at 115 DEG C, described culture medium is added in fermentation tank, then be the saccharomycetic kind of liquid that the inoculum concentration inoculation step 1 of the 5%-10% of described filtrate obtains by quality, 48h is cultivated at the temperature of 28 DEG C, the volume of described fermentation tank is V cubic meter, throughput is (0.4-0.6) V cubic meters per minute, stir, then be the lactobacillus inoculation liquid that the inoculum concentration inoculation step 2 of the 5%-10% of described filtrate obtains by quality, continue to cultivate 5-7d to fermentation ends at the temperature of 37 DEG C, obtain zymotic fluid,
Step 4: centrifugal treating obtains ferment stoste, and zymotic fluid is imported centrifuge, and through the centrifugal 10min of 6000rpm, gained supernatant is ferment stoste;
Step 5: add plant extracts to ferment stoste, be that 20:0.15:14:21:1000 is mixed to join ferment stoste by aloe extract, sucrose, ginger extract, Cordyceps militaris extract, stoste according to mass ratio, 115 degree of 30-50 minute high-temperature sterilizations, carry out packing.
2. a kind of Cordyceps militaris grape ferment preparation method having cosmetic result according to claim 1, is characterized in that: described speed of agitator is 100-120rpm/min.
3. a kind of Cordyceps militaris grape ferment preparation method having cosmetic result according to claim 1, is characterized in that: described lactic acid bacteria is streptococcus thermophilus.
CN201510865364.0A 2015-12-01 2015-12-01 Preparation method of cordyceps militaris and vitis vinifera ferment having cosmetic effect Pending CN105341927A (en)

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Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20080089978A1 (en) * 2006-10-13 2008-04-17 Body Structures, Inc. Lacteal coated pizzas
CN102356899A (en) * 2011-09-07 2012-02-22 中国食品发酵工业研究院 Natural fruit and vegetable ferment beverage, and preparation method thereof
CN103039883A (en) * 2013-01-16 2013-04-17 周礼红 Yangtao lucid ganoderma composite ferment and preparation method thereof
CN103766872A (en) * 2012-10-17 2014-05-07 刘铭 Ferment oral liquid and preparation method thereof
CN103908476A (en) * 2014-04-29 2014-07-09 洛阳奥达特食用菌技术开发有限公司 Cordyceps militaris extract as well as preparation method and preparation thereof
CN103960613A (en) * 2014-05-09 2014-08-06 亳州千草药业有限公司 Method for preparing fruit and vegetable enzymes
CN104784261A (en) * 2015-04-30 2015-07-22 孟令刚 Cistanche enzyme and preparation process thereof

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20080089978A1 (en) * 2006-10-13 2008-04-17 Body Structures, Inc. Lacteal coated pizzas
CN102356899A (en) * 2011-09-07 2012-02-22 中国食品发酵工业研究院 Natural fruit and vegetable ferment beverage, and preparation method thereof
CN103766872A (en) * 2012-10-17 2014-05-07 刘铭 Ferment oral liquid and preparation method thereof
CN103039883A (en) * 2013-01-16 2013-04-17 周礼红 Yangtao lucid ganoderma composite ferment and preparation method thereof
CN103908476A (en) * 2014-04-29 2014-07-09 洛阳奥达特食用菌技术开发有限公司 Cordyceps militaris extract as well as preparation method and preparation thereof
CN103960613A (en) * 2014-05-09 2014-08-06 亳州千草药业有限公司 Method for preparing fruit and vegetable enzymes
CN104784261A (en) * 2015-04-30 2015-07-22 孟令刚 Cistanche enzyme and preparation process thereof

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Application publication date: 20160224