CN103877127A - Application of hirsutella sinensis refined fermentation liquor as bacteriostatic agent of bat moth infection paecilomyces farinosus - Google Patents
Application of hirsutella sinensis refined fermentation liquor as bacteriostatic agent of bat moth infection paecilomyces farinosus Download PDFInfo
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Abstract
The invention relates to the field of prevention and control of animal diseases, and in particular relates to application of hirsutella sinensis refined fermentation liquor as a bacteriostatic agent of bat moth infection paecilomyces farinosus, a paecilomyces farinosus bacteriostatic agent and a preparation method of the paecilomyces farinosus bacteriostatic agent. The bacteriostatic agent provided by the invention can be used for well inhibiting paecilomyces farinosus cultured in vitro and/or in vivo without causing any bad influence on bat moths. Although the bacteriostatic agent is applied continuously to infected moths, hirsutella sinensis in the moths can still live till the moths become stiff. Moreover, stiff moths can form a sporocarp blastema.
Description
The application requires within 19th, to submit in December in 2012 priority that Patent Office of the People's Republic of China, application number are 201210554669.6, denomination of invention is the Chinese patent application of " China pilose spore fermentation liquid refined liquid infects the application of the antibacterial of paecilomycerol as Hepialus armorieanus Oberthur larva " to, and its full content is by reference in conjunction with in this application.
Technical field
The present invention relates to animal diseases prevention and control field, particularly China pilose spore fermentation liquid refined liquid infects the application of the antibacterial of paecilomycerol as Hepialus armorieanus Oberthur larva.
Background technology
Cordyceps is Chinese a kind of rare Chinese medicine, classifies China's three large tonics together with Radix Ginseng, Cornu Cervi Pantotrichum as.Within 7 years, in " Bencao Congxin ", just there is the record of " Cordyceps Gan Pingbao lung, kidney tonifying are mended marrow, and hemostasis and phlegm, phthisical cough, controls diaphragm disease all good " as far back as a Seventh Five-Year Plan.The traditional Chinese medical science thinks, Cordyceps enters lung kidney two warps, can tonifying the lung the moon, again can kidney-replenishing, and cure mainly and suffer from a deficiency of the kidney, impotence and seminal emission, soreness of waist and knee joint, weak after being ill, chronic cough weakness, phthisical cough expectorant blood, spontaneous sweatings etc., are unique a kind of balance simultaneously, the Chinese medicine that regulates negative and positive.
Cordyceps is the organism of a kind of special worm and mycosymbiosis, the mycelium that is Cordyceps fungus (China pilose spore) infects the Hepialus armorieanus Oberthur larva of (Lepidoptera Hepialidae Hepialus armorieanus Oberthur belongs to insecticide) by variety of way, organic substance in its body carries out parasitic life as nutrient energy source, after continuous growth promoter and differentiation, final mycelium is twisted together and is formed Stroma and stretches out host's shell, thus the organism of a kind of special entomogenous fungi symbiosis forming.
Cordyceps main product the grassy marshland area, the local high and cold mountain area of provinces and regions height above sea level 3500～5200m such as in China Tibet, Qinghai, Yunnan, Sichuan and Gansu, and natural resources are very limited, excavates in recent years transition and cause the deterioration of ecological environment, and natural resources are increasingly exhausted.Therefore, artificial culture Cordyceps is imperative.
Artificial culture Cordyceps, infects host insect (Hepialus armorieanus Oberthur larva) by Cordyceps fungus (China pilose spore), enters in host's body cavity, and breeding growth in body cavity, finally produce Stroma, form Cordyceps medical material.
Hepialus armorieanus Oberthur (Hepialidae), the general designation of the nearly 300 kinds of insecticides of Lepidoptera (Lepidoptera) Hepialidae (Hepialidae), distribution on global.Comprise the moth class of several maximums, wing expanse exceedes 22.5 centimeters (9 inch), fly very fast, but without certain orientation.Larva pierces in stem, or lives in the underground root that pastures.It is generally acknowledged, the fungus coalition that every fungi autoeciousness by Cordyceps also can produce sporophore is all called Cordyceps, but the Cordyceps of Chinese traditional Chinese medicine and pharmacy indication is to refer in particular to the cordyceps sinensis bacterium being only distributed in China Qinghai-Tibet Platean and plateau, border district grassy marshland.
In the artificial culture field of Cordyceps, host of Cordyceps sinensis insecticide is Hepialus armorieanus Oberthur larva, often suffers paecilomycerol to infect harm, and Paecilomyces varioti disease is to cause its dead major reason.If this disease spreads, will make Artificial Cultured Cordyceps Sinensis cause heavy losses, therefore to its take effective measures prevent and treat imperative.The control of the current Paecilomyces varioti disease for Hepialus armorieanus Oberthur larva is simple external prevention, under the condition of isolated culture, adopt medicament to suppress, be not that living worm body is tested, and medicament for infect the growth of the China pilose spore strain in rear larva body and larva ossify whether can exert an influence also uncertain.And for the control that in Hepialus armorieanus Oberthur larva body, Paecilomyces varioti infects, also there is no effectively preventing method.If employing chemical prevention and control method, it is residual whether medicament has in Hepialus armorieanus Oberthur larva body, and not yet someone measures.Therefore adopt biological control method to there is certain advantage.
China pilose spore is the phorozoon of Cordyceps, has at present research to claim that its tunning has certain bacteriostasis, escherichia coli and staphylococcus aureus is had to obvious inhibitory action, and there is not yet report for the inhibitory action of fungus.Although the multiple fermentation liquids such as Cordyceps militaris (L.) Link., Cordyceps and Hypocrea virens and Concretio silicea Bambusae seu schizostachyi were all cooked the preliminary study of antibacterial activity, but all just simply measure the inhibitory action of fermentation liquid to certain several antibacterial and fungus, clearly do not indicate the concrete purposes of its mechanism of action and fermentation liquid, in industrial use, also have gap far away with real use.
Summary of the invention
In view of this, the invention provides a kind of refined liquid of China pilose spore fermentation liquid as the application of the antibacterial of Hepialus armorieanus Oberthur larva infection paecilomycerol, paecilomycerol antibacterial and preparation method thereof.Antibacterial provided by the invention has good inhibitory action for the paecilomycerol of external and/or culturing in vivo, and to Hepialus armorieanus Oberthur larva without any harmful effect.For the larva after infecting, although SM, the China pilose spore in polypide still can be survived, until polypide becomes stiff.And Bombyx Batryticatus can form fruit body primordium.
China pilose spore fermentation liquid refined liquid described in the present invention, refers in China pilose spore liquid medium within after fermentation, centrifugal or filter and obtain supernatant, and supernatant, again through means such as evaporation or rotary evaporations, obtains 10-30 fermentation refined liquid doubly.
In order to realize foregoing invention object, the invention provides following technical scheme:
The invention provides China pilose spore fermentation refined liquid infects the antibacterial of paecilomycerol application as Hepialus armorieanus Oberthur larva.
The method of existing inhibition paecilomycerol, often adopts photo-irradiation treatment.Paecilomyces varioti is not strong to the patience of illumination, has impact but Paecilomyces varioti is produced to spore under dark condition, produces spore therefore can raise to control Paecilomyces varioti by appropriate shading.But photo-irradiation treatment is not satisfactory to the inhibition of Paecilomyces varioti.In order to avoid using the chemical reagent of toxicity as far as possible, therefore, the invention provides the application of China pilose spore fermentation refined liquid as the antibacterial of Hepialus armorieanus Oberthur larva infection paecilomycerol.
In some embodiments of the invention, the preparation method of China pilose spore fermentation liquid refined liquid comprises the following steps:
Step 1: get China pilose spore strain and be inoculated in culture medium and ferment, obtain China pilose spore fermentation liquid;
Step 2: get China pilose spore fermentation liquid through centrifugal or filtration, collect filtrate;
Step 3: get the concentrated 10-30 of filtrate doubly, to obtain final product;
Culture medium comprises glucose, peptone, yeast powder, anhydrous magnesium sulfate, potassium dihydrogen phosphate and water, and the mass ratio of described glucose, peptone, yeast powder, anhydrous magnesium sulfate, potassium dihydrogen phosphate and water is 20～32: 6～12: 6～12: 0.4～0.6: 0.8～1.2: 800～1200.
In other embodiment of the present invention, in step 1, fermentation temperature is 15-20 DEG C.
In other embodiment of the present invention, in step 1, fermentation time is 14-25d.
In some embodiments of the invention, in step 3, concentrated temperature is 40-50 DEG C.
The present invention also provides a kind of Hepialus armorieanus Oberthur larva to infect the antibacterial of paecilomycerol, and it includes China pilose spore fermentation liquid refined liquid.
In some embodiments of the invention, a kind of Hepialus armorieanus Oberthur larva provided by the invention infects in the antibacterial of paecilomycerol, and the preparation method of China pilose spore fermentation liquid refined liquid comprises the following steps:
Step 1: get China pilose spore strain and be inoculated in culture medium and ferment, obtain China pilose spore fermentation liquid;
Step 2: get China pilose spore fermentation liquid through centrifugal or filtration, collect filtrate;
Step 3: get the concentrated 10-30 of filtrate doubly, to obtain final product;
Culture medium comprises glucose, peptone, yeast powder, anhydrous magnesium sulfate, potassium dihydrogen phosphate and water, and the mass ratio of described glucose, peptone, yeast powder, anhydrous magnesium sulfate, potassium dihydrogen phosphate and water is 20～32: 6～12: 6～12: 0.4～0.6: 0.8～1.2: 800～1200.
In other embodiment of the present invention, a kind of Hepialus armorieanus Oberthur larva provided by the invention infects in the antibacterial of paecilomycerol, and in step 1, fermentation temperature is 15-20 DEG C.
In other embodiment of the present invention, a kind of Hepialus armorieanus Oberthur larva provided by the invention infects in the antibacterial of paecilomycerol, and in step 1, fermentation time is 14-25d.
In some embodiments of the invention, in step 3, concentrated temperature is 40-50 DEG C.
Add emulsifying agent can make medicine better contact with polypide, therefore, as preferably, antibacterial also comprises emulsifying agent.
As preferably, in antibacterial, the mass fraction of emulsifying agent is 0.1～1.2%.
Preferably, in antibacterial, the mass fraction of emulsifying agent is 0.4～1%.
As preferably, emulsifying agent is a kind of or both above mixture of Tween 80, Polyethylene Glycol (PEG), emulsifier op-10 (condensation substance of alkyl phenol and oxirane).
As preferably, emulsifying agent is Tween 80.
As preferably, the occupation mode of antibacterial provided by the invention is for directly to spray to Hepialus armorieanus Oberthur polypide.
As preferably, every 100 insects are sprayed antibacterial 50-100mL provided by the invention.
The invention provides a kind of China pilose spore fermentation liquid refined liquid and infect application, paecilomycerol antibacterial of the antibacterial of paecilomycerol and preparation method thereof as Hepialus armorieanus Oberthur larva.In Hepialus armorieanus Oberthur larva raising process, if do not find to infect the larva of Paecilomyces varioti, do not process; If while there is infecting the larva of Paecilomyces varioti in colony, susceptible larva is taken out to isolation at once, and other larva is taked to prophylactico-therapeutic measures.When use, first prepare 10-30 China pilose spore fermentation refined liquid doubly, and add emulsifying agent in solution, after mixing, to the spraying of Hepialus armorieanus Oberthur larva body surface, then add appropriate feedstuff to raise.Result of the test shows, antibacterial provided by the invention has good inhibitory action for the paecilomycerol of external and/or culturing in vivo, and to Hepialus armorieanus Oberthur larva without any harmful effect.For the larva after infecting, although SM, the China pilose spore in polypide still can be survived, until polypide becomes stiff.And Bombyx Batryticatus can form fruit body primordium.
Detailed description of the invention
The invention discloses a kind of China pilose spore fermentation liquid refined liquid and infect application, paecilomycerol antibacterial of the antibacterial of paecilomycerol and preparation method thereof as Hepialus armorieanus Oberthur larva, those skilled in the art can use for reference content herein, suitably improve technological parameter and realize.Special needs to be pointed out is, all similar replacements and change apparent to those skilled in the artly, they are all deemed to be included in the present invention.Method of the present invention and application are described by preferred embodiment, related personnel obviously can change methods and applications as herein described in content of the present invention, spirit and scope or suitably change and combination not departing from, and realizes and apply the technology of the present invention.
China pilose spore fermentation liquid refined liquid provided by the invention infects raw materials used in application, paecilomycerol antibacterial of the antibacterial of paecilomycerol and preparation method thereof and reagent and all can be buied by market as Hepialus armorieanus Oberthur larva.
Below in conjunction with embodiment, further set forth the present invention:
Embodiment 1
Prepare fermentation liquid:
China pilose spore strain is inoculated in fermentation medium, and medium component is glucose 500g, peptone 150g, and yeast powder 300g, anhydrous magnesium sulfate 10g, potassium dihydrogen phosphate 20g, water 25L, cultivates 25d in 15 DEG C of 100rpm, obtains fermentation liquid.After fermentation liquid is centrifugal, get supernatant, 40 DEG C of rotary evaporations, are concentrated into 1/10 of original volume, obtain 10 times of refined liquid.
Polypide experiment:
Select infection of Chinese by 60 of the Hepialus armorieanus Oberthur larvas of hair spore bacterium, wherein 30 use 15 times of fermentation refined liquid of China pilose spore to spray to larva body surface, and use amount is every 100 insects spraying 50mL.Other 30 in contrast, use sterilized water processing.Process once every 15d, process 30 still healthy survivals of larva in 4 months post processing groups, find that through sampling microscopy the China pilose spore thalline quantity in larva body is still a lot, be not subject to the impact of medicament.And larva still can be ossify and grow fruit body primordium.And 30 10 of larvas infect Paecilomyces varioti, 20 survivals in matched group.
For the larva after infecting, SM, the China pilose spore in polypide still can be survived, until polypide becomes stiff.And Bombyx Batryticatus can form fruit body primordium.
Embodiment 2
Prepare fermentation liquid:
China pilose spore strain is inoculated in fermentation medium, and medium component is glucose 800g, peptone 300g, and yeast powder 150g, anhydrous magnesium sulfate 15g, potassium dihydrogen phosphate 30g, water 25L, cultivates 14d in 20 DEG C of 100rpm, obtains fermentation liquid.After fermentation liquid is centrifugal, get supernatant, 50 DEG C of rotary evaporations, are concentrated into 1/15 of original volume, obtain 15 times of refined liquid, and weighing, then to add wherein Tween 80 20g be that Tween 80 mass fraction is 1.2%.
Polypide experiment:
Select infection of Chinese by 60 of the Hepialus armorieanus Oberthur larvas of hair spore bacterium, wherein 30 use 30 times of fermentation refined liquid of China pilose spore to spray to larva body surface, and use amount is every 100 insects spraying 100mL.Other 30 in contrast, use sterilized water processing.Process once every 15d, process 30 still healthy survivals of larva in 4 months post processing groups, find that through sampling microscopy the China pilose spore thalline quantity in larva body is still a lot, be not subject to the impact of medicament.And larva still can be ossify and grow fruit body primordium.And 30 11 of larvas infect Paecilomyces varioti, 19 survivals in matched group.
For the larva after infecting, SM, the China pilose spore in polypide still can be survived, until polypide becomes stiff.And Bombyx Batryticatus can form fruit body primordium.
Embodiment 3
Prepare fermentation liquid:
China pilose spore strain is inoculated in fermentation medium, and medium component is glucose 750g, peptone 250g, and yeast powder 250g, anhydrous magnesium sulfate 12.5g, potassium dihydrogen phosphate 25g, water 25L, cultivates 20d in 18 DEG C of 100rpm, obtains fermentation liquid.After fermentation liquid is centrifugal, get supernatant, 45 DEG C of rotary evaporations, are concentrated into 1/10 of original volume, obtain 10 times of refined liquid, and weighing, then to add wherein PEG20g be that PEG mass fraction is 0.8%.
Polypide experiment:
Select infection of Chinese by 60 of the Hepialus armorieanus Oberthur larvas of hair spore bacterium, wherein 30 use 20 times of fermentation refined liquid of China pilose spore to spray to larva body surface, and use amount is every 100 insects spraying 75mL.Other 30 in contrast, use sterilized water processing.Process once every 15d, process 30 still healthy survivals of larva in 4 months post processing groups, find that through sampling microscopy the China pilose spore thalline quantity in larva body is still a lot, be not subject to the impact of medicament.And larva still can be ossify and grow fruit body primordium.And 30 9 of larvas infect Paecilomyces varioti, 21 survivals in matched group.
For the larva after infecting, SM, the China pilose spore in polypide still can be survived, until polypide becomes stiff.And Bombyx Batryticatus can form fruit body primordium.
Embodiment 4
Prepare fermentation liquid:
China pilose spore strain is inoculated in fermentation medium, and medium component is glucose 800g, peptone 200g, and yeast powder 200g, anhydrous magnesium sulfate 10g, potassium dihydrogen phosphate 22.5g, water 20L, cultivates 18d in 16 DEG C of 100rpm, obtains fermentation liquid.After fermentation liquid is centrifugal, get supernatant, 42 DEG C of rotary evaporations, are concentrated into 1/30 of original volume, obtain 30 times of refined liquid.Weigh and then add wherein emulsifier op-10 8.0g, OP-10 mass fraction is 1.2%.
Polypide experiment:
Select infection of Chinese by 60 of the Hepialus armorieanus Oberthur larvas of hair spore bacterium, wherein 30 use 20 times of fermentation refined liquid of China pilose spore to spray to larva body surface, and use amount is every 100 insects spraying 60mL.Other 30 in contrast, use sterilized water processing.Process once every 15d, process 30 still healthy survivals of larva in 4 months post processing groups, find that through sampling microscopy the China pilose spore thalline quantity in larva body is still a lot, be not subject to the impact of medicament.And larva still can be ossify and grow fruit body primordium.And 30 13 of larvas infect Paecilomyces varioti, 17 survivals in matched group.
Embodiment 5
Prepare fermentation liquid:
China pilose spore strain is inoculated in fermentation medium, and medium component is glucose 800g, peptone 300g, and yeast powder 300g, anhydrous magnesium sulfate 15g, potassium dihydrogen phosphate 30g, water 30L, cultivates 20d in 20 DEG C of 100rpm, obtains fermentation liquid.After fermentation liquid is centrifugal, get supernatant, 50 DEG C of rotary evaporations, are concentrated into 1/30 of original volume, obtain 30 times of refined liquid, and weighing, then to add wherein Tween 80 1g be that Tween 80 mass fraction is 0.1%.
Polypide experiment:
Select infection of Chinese by 60 of the Hepialus armorieanus Oberthur larvas of hair spore bacterium, wherein 30 use 30 times of fermentation refined liquid of China pilose spore to spray to larva body surface, and use amount is every 100 insects spraying 80mL.Other 30 in contrast, use sterilized water processing.Process once every 15d, process 30 still healthy survivals of larva in 4 months post processing groups, find that through sampling microscopy the China pilose spore thalline quantity in larva body is still a lot, be not subject to the impact of medicament.And larva still can be ossify and grow fruit body primordium.And 30 11 of larvas infect Paecilomyces varioti, 19 survivals in matched group.
For the larva after infecting, SM, the China pilose spore in polypide still can be survived, until polypide becomes stiff.And Bombyx Batryticatus can form fruit body primordium.
The above is only the preferred embodiment of the present invention; it should be pointed out that for those skilled in the art, under the premise without departing from the principles of the invention; can also make some improvements and modifications, these improvements and modifications also should be considered as protection scope of the present invention.
Claims (14)
1. China pilose spore fermentation liquid refined liquid is as the application of the antibacterial of Hepialus armorieanus Oberthur larva In vivo infection paecilomycerol.
2. application according to claim 1, is characterized in that, the preparation method of described China pilose spore fermentation liquid refined liquid comprises the following steps:
Step 1: get China pilose spore strain and be inoculated in culture medium and ferment, obtain China pilose spore fermentation liquid;
Step 2: get described China pilose spore fermentation liquid through centrifugal or filtration, collect filtrate;
Step 3: get the concentrated 10-30 of described filtrate doubly, to obtain final product;
Described culture medium comprises glucose, peptone, yeast powder, anhydrous magnesium sulfate, potassium dihydrogen phosphate and water, and the mass ratio of described glucose, peptone, yeast powder, anhydrous magnesium sulfate, potassium dihydrogen phosphate and water is 20～32: 6～12: 6～12: 0.4～0.6: 0.8～1.2: 800～1200.
3. application according to claim 2, is characterized in that, fermentation temperature described in step 1 is 15-20 DEG C.
4. application according to claim 2, is characterized in that, fermentation time described in step 1 is 14-25d.
5. application according to claim 2, is characterized in that, described in step 3, concentrated temperature is 40-50 DEG C.
6. Hepialus armorieanus Oberthur larva infects an antibacterial for paecilomycerol, it is characterized in that, it includes China pilose spore fermentation liquid refined liquid.
7. antibacterial according to claim 6, is characterized in that, the preparation method of described China pilose spore fermentation liquid refined liquid comprises the following steps:
Step 1: get China pilose spore strain and be inoculated in culture medium and ferment, obtain China pilose spore fermentation liquid;
Step 2: get described China pilose spore fermentation liquid through centrifugal or filtration, collect filtrate;
Step 3: get the concentrated 10-30 of described filtrate doubly, to obtain final product;
Described culture medium comprises glucose, peptone, yeast powder, anhydrous magnesium sulfate, potassium dihydrogen phosphate and water, and the mass ratio of described glucose, peptone, yeast powder, anhydrous magnesium sulfate, potassium dihydrogen phosphate and water is 20～32: 6～12: 6～12: 0.4～0.6: 0.8～1.2: 800～1200.
8. antibacterial according to claim 7, is characterized in that, fermentation temperature described in step 1 is 15-20 DEG C.
9. antibacterial according to claim 7, is characterized in that, fermentation time described in step 1 is 14-25d.
10. antibacterial according to claim 7, is characterized in that, described in step 3, concentrated temperature is 40-50 DEG C.
11. antibacterial according to claim 6, is characterized in that, it also comprises emulsifying agent.
12. antibacterial according to claim 6, is characterized in that, the mass fraction of emulsifying agent described in described antibacterial is 0.1～1.2%.
13. antibacterial according to claim 6, is characterized in that, described emulsifying agent is a kind of or both the above mixture in Tween 80, Polyethylene Glycol, emulsifier op-10.
14. antibacterial according to claim 6, is characterized in that, its occupation mode is directly spray or be added in the raising substrate of Hepialus armorieanus Oberthur larva to Hepialus armorieanus Oberthur polypide.
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| CN201310697573.XA CN103877127B (en) | 2012-12-19 | 2013-12-18 | China pilose spore fermentation liquid refined liquid infects the application of the antibacterial of paecilomycerol as Hepialus armorieanus Oberthur larva |
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| CN201210554669 | 2012-12-19 | ||
| CN201310697573.XA CN103877127B (en) | 2012-12-19 | 2013-12-18 | China pilose spore fermentation liquid refined liquid infects the application of the antibacterial of paecilomycerol as Hepialus armorieanus Oberthur larva |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2017161917A1 (en) * | 2016-03-22 | 2017-09-28 | Sunshine Lake Pharma Co., Ltd. | Method and system of cultivating cordyceps |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1148623A (en) * | 1996-10-04 | 1997-04-30 | 海南兆隆实业有限公司 | North cordyceps mycelium fermentation technology |
| CN102106235A (en) * | 2010-12-24 | 2011-06-29 | 王梅平 | Total artificial method for cultivating cordyceps sinensis |
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2013
- 2013-12-18 CN CN201310697573.XA patent/CN103877127B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1148623A (en) * | 1996-10-04 | 1997-04-30 | 海南兆隆实业有限公司 | North cordyceps mycelium fermentation technology |
| CN102106235A (en) * | 2010-12-24 | 2011-06-29 | 王梅平 | Total artificial method for cultivating cordyceps sinensis |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2017161917A1 (en) * | 2016-03-22 | 2017-09-28 | Sunshine Lake Pharma Co., Ltd. | Method and system of cultivating cordyceps |
| US10405527B2 (en) | 2016-03-22 | 2019-09-10 | Sunshine Lake Pharma Co., Ltd | Method and system of cultivating cordyceps |
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Denomination of invention: Application of hirsutella sinensis refined fermentation liquor as bacteriostatic agent of bat moth infection paecilomyces farinosus Effective date of registration: 20170511 Granted publication date: 20160817 Pledgee: China Development Bank Co., Ltd.|Hubei Bank Co., Ltd. Yichang Nanhu Sub-branch Pledgor: Yichang mountain water Cordyceps Co., Ltd.|Ruyuan Nanling Mountain good water Cordyceps Co., Ltd. Registration number: 2017990000400 |
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Date of cancellation: 20201230 Granted publication date: 20160817 Pledgee: China Development Bank Co.|Bank of Hubei Limited by Share Ltd. Yichang Nanhu sub branch Pledgor: YICHANG SHANCHENGSHUIDU CORDYCEPS Co.,Ltd.|RUYUAN NANLING HAOSHAN HAOSHUI CORDYCEPS Co.,Ltd. Registration number: 2017990000400 |
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Denomination of invention: Application of purified fermentation broth of trichospora sinensis as an antimicrobial agent for paecilomycetes infection of bat moth larvae Effective date of registration: 20220426 Granted publication date: 20160817 Pledgee: Minmetals International Trust Ltd. Pledgor: YICHANG SHANCHENGSHUIDU CORDYCEPS Co.,Ltd. Registration number: Y2022980004813 |
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