CN103875534B - A kind of tissue culture bottle with light splitting function - Google Patents
A kind of tissue culture bottle with light splitting function Download PDFInfo
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- CN103875534B CN103875534B CN201410118872.8A CN201410118872A CN103875534B CN 103875534 B CN103875534 B CN 103875534B CN 201410118872 A CN201410118872 A CN 201410118872A CN 103875534 B CN103875534 B CN 103875534B
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- 235000005976 Citrus sinensis Nutrition 0.000 claims abstract description 21
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- 210000005239 tubule Anatomy 0.000 claims description 9
- 239000012528 membrane Substances 0.000 claims description 3
- 238000012549 training Methods 0.000 abstract description 20
- 238000007789 sealing Methods 0.000 abstract 1
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- 238000002474 experimental method Methods 0.000 description 1
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- 239000011521 glass Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000029553 photosynthesis Effects 0.000 description 1
- 238000010672 photosynthesis Methods 0.000 description 1
- 238000004161 plant tissue culture Methods 0.000 description 1
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The present invention relates to a kind of tissue culture bottle with light splitting function, comprise the bottle sealing of a pair of bottle is connected, be divided into upper bottle body and lower bottle body, longitudinal section, upper bottle body top is trapezium structure, wherein: trapezoidal inclined plane position outer surface is placed with the refraction structure to upper bottle body central area and royal purple anaclasis to upper bottle body periphery district by the blood orange anaclasis of deflecting light beams, and upper bottle wall outer surface be placed with the refraction structure to upper bottle body and royal purple anaclasis to lower bottle body by the blood orange anaclasis of deflecting light beams; This tissue culture bottle can decompose light and can be by the different parts of different light assign group training seedling.
Description
Technical field
The present invention relates to a kind of tissue culture bottle, particularly a kind of tissue culture bottle with light splitting function.
Background technology
Along with development in science and technology, plant seedling raising technology is variation day by day, and plant tissue culture is fast numerous as a kind of efficient raising technology that can obtain in a short time a large amount of seedlings, remains at present one of most important method of seedling breeding; Research for tissue culture technology concentrates on the exploration to optimal medium on the one hand, is mainly research and the regulation and control of relevant group culture container and group training microenvironment on the other hand, but does not have substantive breakthroughs for the research of group culture container at present always.
Only one of most important factor in photosynthesis of plant process, plant growth mainly absorbs blood orange light and royal purple light, and much studies show that blood orange light promotes that plant shoot divides stem to growth, can effectively improve increment; Royal purple light has dwarfing effect to plant growth, is unfavorable for height growth, but can promote to a certain extent base portion differentiation, thereby improves the rate of increase; Therefore group training artificial light source adopts certain spectral illumination extremely to have meaning.
LED light source can provide specific wave spectrum, but LED light source high cost and light decay phenomenon are serious, practical application still has limitation, the artificial light source of group training is at present taking fluorescent lamp as main, still adopt full spectral illumination, cause great luminous energy waste, increased group training cost, can not realize and maximize propagation simultaneously; In addition, traditional tissue culture bottle adopts top cover-opening structure, and the container light transmittance bringing because bottle cap is aging reduction declines the efficiency of light energy utilization.
Therefore, for the exploitation of novel group of culture container, ensureing on oligosaprobic basis, should make full use of existing group of training environment, comprising artificial light source, expecting to make up by the innovation of group culture container the defect of existing light source; Can wish on the other hand to improve the efficiency of light energy utilization by the light transmittance of the culture container of raising group to greatest extent.
Summary of the invention
In order to overcome the deficiency in background technology, invented a kind of can will entirely compose the tissue culture bottle that disperses of light, allow group training seedling different parts fully obtain being conducive to the irradiation of its growth, its growth rate is significantly improved.
For solving the problems of the technologies described above, the invention provides following technical scheme:
Tissue culture bottle of the present invention comprises the bottle that a pair of bottleneck is tightly connected mutually, be divided into upper bottle body and lower bottle body, longitudinal section, upper bottle body top is trapezium structure, wherein: trapezoidal inclined plane position outer surface is placed with the refraction structure to upper bottle body central area and royal purple anaclasis to upper bottle body periphery district by the blood orange anaclasis in deflecting light beams, and upper bottle wall outer surface be placed with the refraction structure to upper bottle body and royal purple anaclasis to lower bottle body by the blood orange anaclasis in deflecting light beams.
Described refraction structure is the rectangle prism structures of upper-thin-lower-thick, and some rectangle refractive power prism structures with the mode alternative arrangement of upper-thin-lower-thick in trapezoidal inclined plane and upper bottle wall outer surface.
The annulus prism structures that the another kind of refraction structure of the present invention is upper-thin-lower-thick, and annulus prism structures with the mode alternative arrangement of upper-thin-lower-thick in trapezoidal inclined plane and upper bottle wall outer surface.
Rectangle prism structures by upper-thin-lower-thick and annulus prism structures alternative arrangement are in upper bottle body top trapezoidal inclined plane and upper bottle wall outer surface, this structure has similar prism effect, when light is above bottle or when oblique upper is irradiated to this structure, it is to purple some light beams from top to bottom by red that full spectrum is separated into, wherein the prism structures of upper bottle body top trapezoidal inclined plane can separate the royal purple light of the short-and-medium wavelength of full spectrum directive tissue culture bottle sidewall, improves the blood orange light ratio example of direct irradiation on group training seedling; The prism structures of upper bottle body sidewall can separate the blood orange light in full spectrum the first half of the whole tissue culture bottle of directive, the blood orange light ratio example of group training seedling upper end is improved again, and by the latter half of the whole tissue culture bottle of royal purple light separation directive, direct irradiation is reduced in the royal purple light ratio example of plant upper end growing point, group training seedling base portion breaks up the purple light ratio of filling enamel and raises, therefore effectively raising group is trained the seedling speed of growth on the one hand, can improve on the other hand base portion differentiation rate.
As a modification of the present invention, the scope that the scope of the upper thin cut-away section angle β of described rectangle prism structures is made as to the upper thin cut-away section angle theta of 5-30 ° and another program annulus prism structures is 5-30 °, after light is separated, the light that is conducive to group training seedling each several part is distributed more evenly and accurate.
As another kind of improvement of the present invention, the scope of angle α between top bottle top trapezoidal inclined plane and upper bottle wall vertical plane is made as to 20-45 °, make blood orange irradiation greatlyr in the blood orange optical range of upper bottle body central area, to such an extent as to group training seedling can obtain more blood orange light.
As another kind of improvement of the present invention, be non-sharp-pointed arc structure by the corresponding chamfering of described tissue culture bottle bottle upper and lower side, be convenient to clean, can not scratch, simultaneously open an aperture in trapezium structure center of top position, and microstome is connected with a hollow tubule one end, the hollow tubule other end extends in bottle, microstome is filled with gas permeable material with ventilative filter membrane device or hollow tubule inside, convenient ventilative.
As another kind of improvement of the present invention, the material that the top surfaces externally and internally of described upper bottle body trapezium structure and tissue culture bottle inwall are smooth structure and described tissue culture bottle is transparent, light transmissive material, to such an extent as to the light transmittance of raising tissue culture bottle has significantly improved the efficiency of light energy utilization, cost saves lighting.
Brief description of the drawings
Fig. 1 is the tissue culture bottle cross section structure schematic diagram that the present invention has light splitting function;
Fig. 2 A is rectangle prism structures top view of the present invention;
Fig. 2 B is rectangle prism structures left view of the present invention;
Fig. 3 is that rectangle prism structures of the present invention is arranged schematic diagram;
Fig. 4 is annulus Rhizoma Sparganii structural arrangement schematic diagram of the present invention;
Fig. 5 is annulus Rhizoma Sparganii structure longitdinal cross-section diagram of the present invention;
Fig. 6 is that tissue culture bottle rectangle prism structures of the present invention is decomposed and distribution diagram light;
Fig. 7 is that tissue culture bottle annulus prism structures of the present invention is decomposed and distribution diagram light.
Wherein: 1, tissue culture bottle; 2, angle α; 3, trapezoidal inclined plane; 4, upper bottle body; 5, lower bottle body; 6, middle part connecting ring; 7, screw thread; 8, trapezoidal top; 9-12, chamfering; 13, rectangle prism structures; 14, angle β; 15, group training seedling; 16, central area; 17, periphery district; 18, annulus prism structures; 19, angle theta; 20, aperture; 21, hollow tubule.
Detailed description of the invention
Further describe the present invention referring to accompanying drawing and in conjunction with detailed description of the invention, to make those skilled in the art can implement according to this with reference to description word, protection domain of the present invention is not limited to the specific embodiment of the present invention.
As shown in Figure 1, tissue culture bottle 1 of the present invention comprises a pair of cylindrical bottle, and bottleneck periphery is furnished with screw thread 7, also comprise a middle part connecting ring 6, be in circular and middle part connecting ring 6 and be furnished with the screw thread corresponding with bottleneck 7, two bottle body and bottlenecks are screwed in the connecting ring 6 of middle part and are relatively tightly connected by screw thread 7, after forming entirety, be divided into upper bottle body 4 and lower bottle body 5, upper bottle body 4 longitudinal sections, top are trapezium structure, wherein: trapezoidal inclined plane position 3 outer surfaces and upper bottle wall outer surface are placed with refraction structure, described refraction structure is the rectangle prism structures 13 of upper-thin-lower-thick, and some rectangle refractive power prism structures 13 with the mode alternative arrangement of upper-thin-lower-thick in trapezoidal inclined plane 3 and upper bottle wall outer surface, as shown in Figure 3, on bottle, can select zones of different alternative arrangement, also can trapezoidal inclined plane and on a bottle wall outer surface all arrange (unlisted in figure).
As shown in Fig. 4-5, for this programme, refraction structure is not limited to this, and refraction structure can also be the annulus prism structures 18 of upper-thin-lower-thick, and annulus prism structures 18 with the mode alternative arrangement of upper-thin-lower-thick in trapezoidal inclined plane 3 and upper bottle wall outer surface.
Draw through inventor's experiment, the scope that is the upper thin cut-away section angle theta 19 of annulus prism structures 18 in 5-30 ° and the other scheme of Fig. 5 as the scope of the upper thin cut-away section angle β 14 of rectangle prism structures 13 in Fig. 2 A and Fig. 2 B is 5-30 °, after light is separated, the light that is conducive to group training seedling each several part is distributed more evenly and accurate, more preferably, shown in Fig. 1, the scope of angle α 2 between tissue culture bottle upper bottle body 4 top trapezoidal inclined planes 3 and upper bottle wall vertical plane is made as to 20-45 °, make blood orange irradiation larger in the blood orange optical range of upper bottle body central area, to such an extent as to that group training seedling can obtain the ratio of blood orange light is higher, and by corresponding tissue culture bottle bottle upper and lower side chamfering (9, 10, 11, 12) be made as non-sharp-pointed arc structure, this structure allows tissue culture bottle be convenient to clean, can not scratch hand, open an aperture in trapezium structure center of top position simultaneously, and microstome is connected with a hollow tubule one end, the hollow tubule other end extends in bottle, microstome with ventilative filter membrane device (as, air-tightness filter screen etc.) or hollow tubule in be filled with gas permeable material (as, cotton etc.), convenient ventilative.
Rectangle prism structures by upper-thin-lower-thick and annulus prism structures alternative arrangement are in upper bottle body top trapezoidal inclined plane and upper bottle wall outer surface, this structure has similar prism effect, when light is above bottle or when oblique upper is irradiated to this structure, it is to purple some light beams from top to bottom by red that full spectrum is separated into, wherein the prism structures of upper bottle body top trapezoidal inclined plane can separate directive tissue culture bottle sidewall (as shown in Fig. 6-7) by the royal purple light of the short-and-medium wavelength of full spectrum, improves the blood orange light ratio example of direct irradiation on group training seedling; The prism structures of upper bottle body sidewall can separate the blood orange light in full spectrum the first half (as shown in Fig. 6-7) of the whole tissue culture bottle of directive, the blood orange light ratio example of group training seedling upper end is raise, and by the latter half (as shown in Fig. 6-7) of the whole tissue culture bottle of royal purple light separation directive, direct irradiation is reduced in the royal purple light ratio example of plant upper end growing point, group training seedling base portion breaks up the purple light ratio of filling enamel and raises, therefore effectively raising group is trained the seedling speed of growth on the one hand, can improve on the other hand base portion differentiation rate.
At tissue culture bottle 1 duration of test, find that it is that transparent, light transmissive material is (as glass that upper bottle body 4 trapezium structure top 8 surfaces externally and internallies and tissue culture bottle 1 inwall are all made as to the material of smooth structure and described tissue culture bottle 1 and the material of middle part connecting ring 6, transparent, smooth plastics etc.), the light transmittance of tissue culture bottle is obviously improved, significantly improved the efficiency of light energy utilization, cost saves lighting.
As shown in Fig. 6-7, for in use light distribution diagram of this tissue culture bottle 1, the rectangle prism structures 13 of upper bottle body trapezoidal inclined plane 3 and annulus prism structures 18 can be assigned to blood orange light the central area 16 of upper bottle body 4, rectangle Rhizoma Sparganii structure 13 and annulus prism structures 18 that royal purple light is assigned to upper bottle body 4 periphery districts 17 and upper bottle wall can be assigned to royal purple light lower bottle body 5, and group training seedling base portion germination percentage and group training seedling growth rate are significantly improved.
Claims (6)
1. there is a tissue culture bottle for light splitting function, comprise the bottle that a pair of bottleneck is tightly connected mutually, pointFor upper bottle body and lower bottle body, longitudinal section, upper bottle body top is trapezium structure, it is characterized in that: trapezoidal inclined planePosition outer surface is placed with the blood orange anaclasis in deflecting light beams to upper bottle body central area and royal purple anaclasisTo the refraction structure in upper bottle body periphery district, and upper bottle wall outer surface is placed with the blood orange in deflecting light beamsThe refraction structure of lower bottle body is arrived in anaclasis to upper bottle body and royal purple anaclasis;
Described refraction structure is the rectangle prism structures of upper-thin-lower-thick, and some rectangle refractive power prisms knotStructure with the mode alternative arrangement of upper-thin-lower-thick in trapezoidal inclined plane and upper bottle wall outer surface;
Wherein, between upper bottle body top trapezoidal inclined plane and upper bottle wall vertical plane, the scope of angle α is 20-45 °.
2. the tissue culture bottle according to claim 1 with light splitting function, is characterized in that: described refractionStructure can also be the annulus prism structures of upper-thin-lower-thick, and annulus prism structures is with upper-thin-lower-thickMode alternative arrangement is in trapezoidal inclined plane and upper bottle wall outer surface.
3. according to the tissue culture bottle described in claim 1-2 any one with light splitting function, it is characterized in that:The corresponding chamfering of described tissue culture bottle bottle upper and lower side is non-sharp-pointed arc structure.
4. according to the tissue culture bottle described in claim 1-2 any one with light splitting function, it is characterized in that:Open an aperture in trapezium structure center of top position, and microstome is connected with a hollow tubule one end, hollowThe tubule other end extends in bottle, and microstome is with being filled with in ventilative filter membrane device or hollow tubuleGas material.
5. according to the tissue culture bottle described in claim 1-2 any one with light splitting function, it is characterized in that:Top surfaces externally and internally and the tissue culture bottle inwall of described upper bottle body trapezium structure are smooth structure.
6. according to the tissue culture bottle described in claim 1-2 any one with light splitting function, it is characterized in that:The material of described tissue culture bottle is transparent, light transmissive material.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410118872.8A CN103875534B (en) | 2013-11-29 | 2014-03-27 | A kind of tissue culture bottle with light splitting function |
Applications Claiming Priority (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310629481.8A CN103688853A (en) | 2013-11-29 | 2013-11-29 | Tissue culture flask with light splitting function |
| CN2013106294818 | 2013-11-29 | ||
| CN201310629481.8 | 2013-11-29 | ||
| CN201410118872.8A CN103875534B (en) | 2013-11-29 | 2014-03-27 | A kind of tissue culture bottle with light splitting function |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103875534A CN103875534A (en) | 2014-06-25 |
| CN103875534B true CN103875534B (en) | 2016-05-04 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310629481.8A Withdrawn CN103688853A (en) | 2013-11-29 | 2013-11-29 | Tissue culture flask with light splitting function |
| CN201410118872.8A Active CN103875534B (en) | 2013-11-29 | 2014-03-27 | A kind of tissue culture bottle with light splitting function |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310629481.8A Withdrawn CN103688853A (en) | 2013-11-29 | 2013-11-29 | Tissue culture flask with light splitting function |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN202046501U (en) * | 2011-04-29 | 2011-11-23 | 中水(上海)饮料有限公司 | Beverage bottle of form with hexagonal prism surface |
| CN102433259A (en) * | 2011-12-16 | 2012-05-02 | 湖北民族学院 | Full-wavelength light source controllable biological culture device |
| CN202575008U (en) * | 2012-05-30 | 2012-12-05 | 陈飞伍 | Multi-pyramid conical surface refraction bottle |
| CN103851581A (en) * | 2012-11-28 | 2014-06-11 | 深圳市海洋王照明工程有限公司 | Lamp holder component |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR100954054B1 (en) * | 2008-05-14 | 2010-04-20 | 롯데알미늄 주식회사 | PET beverage bottle |
| CN203851581U (en) * | 2013-11-29 | 2014-10-01 | 广西壮族自治区农业科学院花卉研究所 | Plant tissue-culture container |
-
2013
- 2013-11-29 CN CN201310629481.8A patent/CN103688853A/en not_active Withdrawn
-
2014
- 2014-03-27 CN CN201410118872.8A patent/CN103875534B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN202046501U (en) * | 2011-04-29 | 2011-11-23 | 中水(上海)饮料有限公司 | Beverage bottle of form with hexagonal prism surface |
| CN102433259A (en) * | 2011-12-16 | 2012-05-02 | 湖北民族学院 | Full-wavelength light source controllable biological culture device |
| CN202575008U (en) * | 2012-05-30 | 2012-12-05 | 陈飞伍 | Multi-pyramid conical surface refraction bottle |
| CN103851581A (en) * | 2012-11-28 | 2014-06-11 | 深圳市海洋王照明工程有限公司 | Lamp holder component |
Also Published As
| Publication number | Publication date |
|---|---|
| CN103875534A (en) | 2014-06-25 |
| CN103688853A (en) | 2014-04-02 |
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Effective date of registration: 20230105 Address after: No.174, Daxue East Road, Nanning, Guangxi 530000 Patentee after: GUANGXI ACADEMY OF AGRICULTURAL SCIENCES Address before: No. 174, Daxue East Road, XiXiangTang District, Nanning City, Guangxi Zhuang Autonomous Region Patentee before: FLOWER Research Institute GUANGXI ACADEMY OF AGRICULTURAL SCIENCES |