CN103837510A - Fluorescence measurement cavity, system and method thereof - Google Patents
Fluorescence measurement cavity, system and method thereof Download PDFInfo
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- CN103837510A CN103837510A CN201310003586.2A CN201310003586A CN103837510A CN 103837510 A CN103837510 A CN 103837510A CN 201310003586 A CN201310003586 A CN 201310003586A CN 103837510 A CN103837510 A CN 103837510A
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- 238000005259 measurement Methods 0.000 title claims abstract description 46
- 238000000034 method Methods 0.000 title abstract description 13
- 239000000523 sample Substances 0.000 claims description 25
- 238000002795 fluorescence method Methods 0.000 claims description 21
- 239000013307 optical fiber Substances 0.000 claims description 17
- 239000000463 material Substances 0.000 claims description 15
- 238000004611 spectroscopical analysis Methods 0.000 claims description 14
- 238000009792 diffusion process Methods 0.000 claims description 12
- TZCXTZWJZNENPQ-UHFFFAOYSA-L barium sulfate Chemical compound [Ba+2].[O-]S([O-])(=O)=O TZCXTZWJZNENPQ-UHFFFAOYSA-L 0.000 claims description 10
- 230000010354 integration Effects 0.000 claims description 9
- 230000000149 penetrating effect Effects 0.000 claims description 9
- 230000000295 complement effect Effects 0.000 claims description 4
- 229910044991 metal oxide Inorganic materials 0.000 claims description 4
- 150000004706 metal oxides Chemical class 0.000 claims description 4
- 239000004065 semiconductor Substances 0.000 claims description 4
- 238000004458 analytical method Methods 0.000 claims 2
- 238000000691 measurement method Methods 0.000 abstract 1
- 238000010586 diagram Methods 0.000 description 18
- 230000005284 excitation Effects 0.000 description 17
- 229930002875 chlorophyll Natural products 0.000 description 11
- 235000019804 chlorophyll Nutrition 0.000 description 11
- ATNHDLDRLWWWCB-AENOIHSZSA-M chlorophyll a Chemical compound C1([C@@H](C(=O)OC)C(=O)C2=C3C)=C2N2C3=CC(C(CC)=C3C)=[N+]4C3=CC3=C(C=C)C(C)=C5N3[Mg-2]42[N+]2=C1[C@@H](CCC(=O)OC\C=C(/C)CCC[C@H](C)CCC[C@H](C)CCCC(C)C)[C@H](C)C2=C5 ATNHDLDRLWWWCB-AENOIHSZSA-M 0.000 description 11
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- 230000037039 plant physiology Effects 0.000 description 1
- 230000009131 signaling function Effects 0.000 description 1
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Abstract
The invention discloses a fluorescence measurement cavity, a system and a method thereof. The fluorescence measuring cavity comprises a body and an inner emitting surface. The body is provided with at least one signal window, and the inner shooting surface is arranged on the inner wall of the body. In another embodiment, the fluorescence measurement cavity may be integrated into a fluorescence measurement system, which includes at least one light source control module, at least one spectrometer, at least one fluorescence measurement cavity, and at least one central control module. The fluorescence measuring cavity is respectively coupled with the at least one spectrometer and the at least one light source control module, and the central control module is respectively electrically connected with the at least one spectrometer and the at least one light source control module. In addition, in another embodiment, a fluorescence measurement method is provided, in which a fluorescence signal generated by at least one object to be measured excited by a light is measured by a fluorescence measurement system, and then analyzed to generate fluorescence information.
Description
Technical field
The present invention relates to a kind of technology that measures cavity and system and its method, especially relate to a Plants fluorescence volume and survey cavity and system and its method.
Background technology
In existing single-point plant fluorescence volume test aircraft structure, metering system need be with each single sample, and the mode measuring is one by one carried out, and causes life period between metric data to postpone, and also represents and in metric data, comprises micro-physiological effect and error between the individuality that the time causes.Therefore, measure multiple samples if want simultaneously, must set up multiple independent quantities measurement units, build cost and also significantly rise thereupon.
In addition, many fluorescence signal functions that only can possess measurement single component in standing crop test aircraft structure, for example: measure chlorophyll fluorescence signal, but in plant individual, there is multiple heterogeneity, can pass through different excitation sources, produce corresponding fluorescence signal in different reaction wave bands, possess the analytic ability of full spectrum if measure framework, can lifting capacity examining system be applied to the potentiality of multiple research and demand.
Therefore, the present invention will set up the complete stool plant fluorescence volume examining system that can carry out synchronous, instant, multisample and full spectrum, it has non-time delay, must not consider to cause between individuality micro-physiological effect and obtain more single number of sheets according to more representative complete stool plant chlorophyll fluorescence information because of the time.
Summary of the invention
The object of the present invention is to provide a kind of fluorescence to measure cavity, in order to make complete stool plant evenly be subject to light via total reflection or perfect diffusion exciting light, and retain complete stool plant and be excited fluorescence signal that light excites in cavity.
A further object of the present invention is to propose a kind of fluorescence volume examining system, and the complete stool plant fluorescence of synchronous in order to carry out, instant, multisample and full spectrum measures.
Another object of the present invention is to propose a kind of fluorescence method for measurement, there is the non-time delay of measurement with the complete complete stool plant chlorophyll fluorescence information that obtains.
For reaching above-mentioned purpose, the present invention proposes a kind of fluorescence and measures cavity, and it comprises in a body and one penetrates face.Body has at least one signal window.Inside penetrate the inwall that face is arranged at body.
The present invention also proposes a kind of fluorescence volume examining system, and it comprises that at least one light source control module, at least one spectrometer, at least one fluorescence measure cavity and at least one central control module.At least one fluorescence measures cavity and is respectively coupled at least one spectrometer and at least one light source control module.Wherein each fluorescence measures cavity and has in a body and one and penetrate face.Body has at least one signal window.Inside penetrate the inwall that face is arranged at body.At least one central control module is electrically connected on respectively at least one spectrometer and at least one light source control module.
The present invention also proposes a kind of fluorescence method for measurement, it includes the following step: provide at least one fluorescence to measure cavity, it has a body and on body, offers the inwall of penetrating face at least one signal window and one and be arranged at body, at least one determinand is arranged at least one fluorescence and measures in cavity; At least one light source control module is provided, and it launches an exciting light at least one fluorescence measures cavity; And at least one spectrometer is provided, it measures that at least one determinand is excited that light excites and the fluorescence signal that produces, and then analyzes and produce a fluorescence information.
Brief description of the drawings
Fig. 1 is that the fluorescence of the embodiment of the present invention measures cavity schematic diagram;
Fig. 2 A is the fluorescence volume examining system schematic diagram of first embodiment of the invention;
Fig. 2 B is the fluorescence volume examining system schematic diagram of second embodiment of the invention;
Fig. 2 C is the fluorescence volume examining system schematic diagram of third embodiment of the invention;
Fig. 3 A is the fluorescence volume examining system schematic diagram of fourth embodiment of the invention;
Fig. 3 B is the fluorescence volume examining system schematic diagram of fifth embodiment of the invention;
Fig. 4 is that the fluorescence of Fig. 2 A of the present invention measures method flow diagram;
Fig. 5 is that another fluorescence of Fig. 2 A of the present invention measures method flow diagram;
Fig. 6 is that the fluorescence of Fig. 3 A of the present invention measures method flow diagram.
Main element symbol description
20-fluorescence volume examining system
200A-200E-first～five fluorescence measures cavity
202A-202E-first～five body
In 204A-204E-first～five, penetrate face
206A-206E-first～five signal window
210A-the first light source control module
212A-the first light source control unit
214A-the first excitation source
210B-secondary light source control module
212B-secondary light source control module
214B-the second excitation source
210D-the 4th light source control module
212D-the 4th light source control unit
214D-the 4th excitation source
220A-the first spectrometer
222A-the first spectroscopy unit
224A-the first sense channel module
226A-the first sensing optical fiber
228A-the first sensing probe
220D-the 4th spectrometer
222D-the 4th spectroscopy unit
224D-the 4th sense channel module
226C-the 4th sensing optical fiber
228C-the 4th sensing probe
230A-the first central control module
230D-the 4th central control module
240-exciting light
250-determinand
260-fluorescence signal
30-fluorescence method for measurement
Step S31-step S33
40-fluorescence method for measurement
Step S41-step S45
50-fluorescence method for measurement
Step S51-step S55
Embodiment
Fig. 1 is that the fluorescence of the embodiment of the present invention measures cavity schematic diagram, and fluorescence measures cavity 100, and it comprises in a body 102 and one penetrates face 104.Body 102 has at least one signal window 106, and the present embodiment is three signal windows 106, but not as limit, wherein signal window 106 can be used as fluorescence and measures the signal input of cavity 100 and the interface of output.In one embodiment, each signal window 106 can be also a hole.Inside penetrate the inwall that face 104 is arranged at body 102, inside penetrating face 104 can be a curved surface, a concave surface, a convex surface or a plane.Inside penetrating face 104 can be formed or be coated with by a total reflection material or a perfect diffusion material, wherein, in one embodiment, can be made up of a barium sulphate material optical effect of total reflection or perfect diffusion, but not as limit.In addition, fluorescence measurement cavity 100 can be also an integrating sphere, an integration rod or an integral operation module.In the present embodiment, fluorescence measurement cavity 100 is an integrating sphere.
Fig. 2 A is the fluorescence volume examining system schematic diagram of first embodiment of the invention, fluorescence volume examining system 20, and it comprises that one first light source control module 210A, one first spectrometer 220A, one first fluorescence measure cavity 200A and one first central control module 230A.Fluorescence measures cavity 200A and is respectively coupled to the first spectrometer 220A and the first light source control module 210A, and wherein the first fluorescence measures cavity 200A and has in a first noumenon 202A and one first and penetrate face 204A.The first noumenon 202A has three first signal window 206A, but not as limit, as long as at least one can be implemented.Wherein first signal window 206A can be used as the first fluorescence and measures the signal input of cavity 200A and the interface of output.In one embodiment, each first signal window 206A can be also a hole.In first, penetrate the inwall that face 204A is arranged at the first noumenon 202A, penetrating face 204A in first can be a curved surface, a concave surface, a convex surface or a plane.In first, penetrating face 204A can be formed by a total reflection material or a perfect diffusion material, wherein, in one embodiment, can be made up of a barium sulphate material optical effect of total reflection or perfect diffusion, but not as limit.In addition, the first fluorescence measurement cavity 200A can be also an integrating sphere, an integration rod or an integral operation module.In the present embodiment, the first fluorescence measurement cavity 200A is an integrating sphere.The first central control module 230A is electrically connected on respectively the first spectrometer 220A and the first light source control module 210A.The first light source control module 210A has one first light source control unit 212A and three the first excitation source 214A.One end of the first light source control unit 212A is connected with the first central control module 230A, each the first excitation source 214A is connected in the other end of the first light source control unit 212A, and be respectively coupled to first signal window 206A, and launch in exciting light 240 to first fluorescence measurement cavity 200A.In addition, fluorescence volume examining system 20 also has a determinand 250, determinand 250 is arranged at the first fluorescence and measures in cavity 200A, determinand 250 can be a complete stool plant or a plant leaf, in the present embodiment, determinand 250 is a complete stool plant, and is excited by the exciting light 240 of the first excitation source 214A transmitting and produce a chlorophyllous fluorescence signal 260.In addition, the first excitation source 214A can be also a pulse type laser or a light emitting diode, but not as limit.The first spectrometer 220A has one first spectroscopy unit 222A and three the first sense channel module 224A.Each first sense channel module 224A has one first sensing optical fiber 226A and one first sensing probe 228A, the two ends of the first sensing optical fiber 226A are connected to the first spectroscopy unit 222A and the first sensing probe 228A, and the first sensing probe 228A is coupled to first signal window 206A, and measure that determinand 250 in the first fluorescence measures cavity 200A is excited by the exciting light 240 of the first excitation source 214A transmitting and the chlorophyll fluorescence signal 260 that produces, via the first sensing optical fiber 226A, chlorophyll fluorescence signal 260 is sent to the first spectroscopy unit 222A and produces a chlorophyll fluorescence information to analyze, in the present embodiment, chlorophyll fluorescence information is the full spectrum of chlorophyll.In addition, the first sensing probe 228A can be also a charge coupled device (Charge Coupled Device, CCD) or one complementary metal oxide semiconductor (CMOS) (Complementary Metal Oxide Semiconductor, CMOS), but not as limit.In the present embodiment, fluorescence volume examining system 20 is the small-sized complete stool plant fluorescence volume examining system of a kind of single sample and full spectrum.
Be noted that, although in the above-described embodiments, the first light source control module 210A, the first spectrometer 220A, the quantity that the first fluorescence measures cavity 200A and the first central control module 230A is one, the quantity of the first light source control unit 212A is that the quantity of one and the first excitation source 214A is three, the quantity of the first spectroscopy unit 222A is that the quantity of one and the first sense channel module 226A is three, but the quantity of in fact each unit can reference measure demand, in fluorescence volume examining system 20, increase multiple unit, to reach user's optimum measurement effect.
As Fig. 2 B fluorescence volume examining system schematic diagram that is second embodiment of the invention.The framework of the present embodiment is substantially similar to the framework shown in Fig. 2 A, difference be the fluorescence volume examining system 20 of the present embodiment, it also includes and measures with the first fluorescence one second fluorescence that cavity 200A structure is identical and measure cavity 200B and the identical secondary light source control module 210B of the first light source control module 210A structure.Wherein the second fluorescence measures cavity 200B and has in one second body 202B and one second and penetrate face 204B.The second body 202B has three secondary signal window 206B, but not as limit, as long as at least one can be implemented.Secondary light source control module 210B has a secondary light source control module 212B and three the second excitation source 214B.The first spectrometer 220A also has one second sensing optical fiber 226B and the one second sensing probe 228B identical with the first sensing optical fiber 226A and the first sensing probe 228A structure.Wherein one end of secondary light source control module 212B is connected with the first light source control unit 212A, and each second excitation source 214B is connected in the other end of secondary light source control module 212B, and is respectively coupled to secondary signal window 206B.The two ends of the second sensing optical fiber 226B are connected to the first spectroscopy unit 222A and the second sensing probe 228B, and the second sensing probe 228B is coupled to secondary signal window 206B.Be noted that in the present embodiment, fluorescence volume examining system 20 is the small-sized complete stool plant fluorescence volume examining system of a kind of synchronous, instant, multisample and full spectrum.
As Fig. 2 C fluorescence volume examining system schematic diagram that is third embodiment of the invention.The framework of the present embodiment is substantially similar to the framework shown in Fig. 2 A, difference be the fluorescence volume examining system 20 of the present embodiment, it also includes with the first fluorescence and measures one the 3rd fluorescence measurement cavity 200C that cavity 200A structure is identical, and wherein the 3rd fluorescence measurement cavity 200C is an integration rod.Be noted that in the present embodiment, fluorescence volume examining system 20 utilizes integration rod to measure cavity as fluorescence can measure large-scale complete stool plant, and it is the large-scale complete stool plant fluorescence volume examining system of a kind of single sample and full spectrum.
As Fig. 3 A fluorescence volume examining system schematic diagram that is fourth embodiment of the invention.The framework of the present embodiment is substantially similar to the framework shown in Fig. 2 A, difference be the fluorescence volume examining system 20 of the present embodiment, it also includes and measures with the first fluorescence three the 4th fluorescence that cavity 200A structure is identical and measure one the 4th spectrometer 220D that cavity 200D, one the 4th light source control module 210D, the first spectrometer 220A structure that the first light source control module 210A structure is identical are identical and identical one the 4th central control module 230D of the first central control module 230A structure.Wherein each the 4th fluorescence measures cavity 200D and has in one the 4th body 202D and one the 4th and penetrate face 204D.Each the 4th body 202D has one the 4th signal window 206D, but not as limit.The 4th light source control module 210D has one the 4th light source control unit 212D and three the 4th excitation source 214D.The 4th spectrometer 220D has three the 4th sensing optical fiber 226D and three the 4th sensing probe 228D.Wherein one end of the 4th light source control unit 212D is connected with the 4th central control module 230D, each the 4th excitation source 214D is connected in the other end of the 4th light source control unit 212D, and is respectively coupled to the 4th signal window 206D of each the 4th fluorescence measurement cavity 200D.The two ends of the 4th sensing optical fiber 226D are connected to the 4th spectroscopy unit 222D and the 4th sensing probe 228D, and the 4th sensing probe 228D is respectively coupled to the 4th signal window 206D of each the 4th fluorescence measurement cavity 200D.Be noted that in the present embodiment, fluorescence volume examining system 20 is the small-sized complete stool plant fluorescence volume examining system of a kind of synchronous, instant, multisample and full spectrum.
As Fig. 3 B fluorescence volume examining system schematic diagram that is fifth embodiment of the invention.The framework of the present embodiment is substantially similar to the framework shown in Fig. 3 A, difference be the fluorescence volume examining system 20 of the present embodiment, it also includes with the 4th fluorescence and measures three the 5th fluorescence measurement cavity 200E that cavity 200D structure is identical, and it is an integration rod that each the 5th fluorescence measures cavity 200E.Be noted that in the present embodiment, fluorescence volume examining system 20 utilizes integration rod to measure cavity as fluorescence can measure large-scale complete stool plant, and it be a kind of synchronous, instant, multisample and the large-scale complete stool plant fluorescence volume examining system of spectrum entirely.
Fig. 4 is that the fluorescence of Fig. 2 A of the present invention measures method flow diagram.A kind of fluorescence method for measurement 30, it includes the following step: step S31, provide one first fluorescence to measure cavity 200A, it has a first noumenon 202A and on the first noumenon 202A, offers the inwall of penetrating face 204A in three first signal window 206A and one first and be arranged at the first noumenon 202A, a determinand 250 is arranged to the first fluorescence and measures in cavity 200A; Step S32, provides one first light source control module 210A, and it launches an exciting light 240 in the first fluorescence measures cavity 200A; And step S33, one first spectrometer 220A is provided, it measures that determinand 250 is excited that light 240 excites and the fluorescence signal 260 that produces, and then analyzes and produce a fluorescence information.Be noted that determinand 250 can be a complete stool plant or a plant leaf, in the present embodiment, determinand 250 is a complete stool plant, and is excited by the exciting light 240 of the first excitation source 214A transmitting and produce a chlorophyllous fluorescence signal 260.
Fig. 5 is that another fluorescence of Fig. 2 A of the present invention measures method flow diagram.A kind of fluorescence method for measurement 40, it includes the following step: step S41, provide one first fluorescence to measure cavity 200A, it has a first noumenon 202A and on the first noumenon 202A, offers the inwall of penetrating face 204A in three first signal window 206A and one first and arrange the first noumenon 202A, determinand 250 is arranged to the first fluorescence and measures in cavity 200A; Step S42, provides one first central control module 230A and one first light source control module 210A, and the first central control module 230A also drives the first light source control module 210A to launch exciting light 240 to first fluorescence and measures in cavity 200A; Step S43, the first fluorescence measure cavity 200A first in penetrate face 204A and make determinand 250 evenly be subject to light via a total reflection or a perfect diffusion exciting light 240, determinand 250 total reflection or the perfect diffusion that fluorescence signal 260 that light 240 excites can measure cavity 200A by the first fluorescence that be excited remain in cavity; Step S44, one first spectrometer 220A is provided, wherein the first central control module 230A also drives the first spectrometer 220A to measure that the first fluorescence measures that determinand 250 in cavity 200A is excited that light excites and the fluorescence signal 260 that produces, and then analyzes generation one fluorescence information; And step S45, provide fluorescence more than one to measure cavity modes, many fluorescence measures cavity modes the first spectrometer 220A is analyzed produce determinand 250 to be excited region-wide fluorescence information mean value that light 240 excites and the determinand 250 zones of different fluorescence information numerical value that light 240 excites that is excited.Be noted that determinand 250 can be a complete stool plant or a plant leaf, in the present embodiment, determinand 250 is a complete stool plant, and is excited by the exciting light 240 of the first excitation source 214A transmitting and produce a chlorophyllous fluorescence signal 260.
Fig. 6 is that the fluorescence of Fig. 3 A of the present invention measures method flow diagram.A kind of fluorescence method for measurement 50, it includes the following step: step S51, provide three the 4th fluorescence to measure cavity 200D, each the 4th fluorescence measures cavity 200D to be had one the 4th body 202D and on the 4th body 202D, offers the inwall of penetrating face 204D in three the 4th signal window 206D and one the 4th and arrange the 4th body 202D, three determinands 250 is arranged to the 4th fluorescence and measures in cavity 200D; Step S52, provides one the 4th central control module 230D and one the 4th light source control module 210D, and the 4th central control module 230D also drives the 4th light source control module 210D to launch an exciting light 240 to each the 4th fluorescence and measures in cavity 200D; Step S53, the 4th fluorescence measure cavity 200D the 4th in penetrate face 204D and make each determinand 250 evenly be subject to light via a total reflection or a perfect diffusion exciting light 240, total reflection or the perfect diffusion that fluorescence signal 260 that light 240 excites can measure cavity 200D by the 4th fluorescence that be excited of each determinand 250 remain in cavity; Step S54, one the 4th spectrometer 220D is provided, wherein the 4th central control module 230D also drives the 4th spectrometer 220D to measure that each the 4th fluorescence measures that determinand 250 in cavity 200D is excited that light 240 excites and the fluorescence signal 260 that produces, and then analyzes and produce a fluorescence information; And step S55, provide a single fluorescence to measure cavity modes, single fluorescence measures cavity modes and the 4th spectrometer 220D is analyzed result from the 4th fluorescence to measure be excited region-wide fluorescence information mean value that light 240 excites and another the 4th fluorescence of determinand 250 in cavity 200D to measure determinand 250 in the cavity 200D region-wide fluorescence information mean value that light 240 excites that is excited.Be noted that determinand 250 can be a complete stool plant or a plant leaf, in the present embodiment, determinand 250 is a complete stool plant, and is excited by the exciting light 240 of each the 4th excitation source 214D transmitting and produce a chlorophyllous fluorescence signal 260.
In sum, be of the present inventionly applied to the System and method for that chlorophyll fluorescence measures, the spectrometer application front end sensing optical fiber by multiple sense channel modules and sensing probe are as sensing element.Coordinate the light source control module of fluorescence volume examining system, the spectrometer of these multiple sense channel modules is after receiving the chlorophyll fluorescence signal of full spectrum, to be used for analyzing its feature crest, to produce chlorophyll fluorescence information or other composition subject matter content of complete stool plant.Compared to prior art, utilize this kind of measuring technique, only need the spectrometer of multiple sense channel modules, can possess the measuring function of many spectrometers, therefore, not only can effectively reduce building cost, possessing full spectrum resolution ability of system, also can synchronously carry out instant multisample measurement, further, by measured data, as the control parameter of environment control element, Greenhouse System is controlled in the environmental parameter back coupling that construction goes out based on plant physiology accordingly.
Above-described specific embodiment, only release Characteristic of the present invention for example, but not for limiting the category of implementing of the present invention, in not departing under the spirit and technology category of taking off in the present invention, the disclosed content of any utilization and the equivalence that completes changes and modify, all still should be above-mentioned claim and contains.
Claims (27)
1. fluorescence measures a cavity, and it comprises:
Body, it has at least one signal window; And
Inside penetrate face, it is arranged at the inwall of this body.
2. fluorescence as claimed in claim 1 measures cavity, and wherein this each signal window is a hole.
3. fluorescence as claimed in claim 1 measures cavity, wherein this interior penetrate face be a curved surface, a concave surface, a convex surface or a plane one of them.
4. fluorescence as claimed in claim 3 measures cavity, and wherein this is interiorly penetrated face one of them is coated with by a barium sulphate material, a total reflection material or a perfect diffusion material.
5. fluorescence as claimed in claim 1 measures cavity, wherein this body be an integrating sphere, an integration rod or an integral operation module one of them.
6. a fluorescence volume examining system, it comprises:
At least one light source control module;
At least one spectrometer;
At least one fluorescence measures cavity, and it is respectively coupled to this at least one spectrometer and this at least one light source control module, and wherein this each fluorescence measurement cavity has:
Body, it has at least one signal window; And
Inside penetrate face, it is arranged at the inwall of this body; And
At least one central control module, it is electrically connected on respectively this at least one spectrometer and this at least one light source control module.
7. fluorescence volume examining system as claimed in claim 6, wherein this each light source control module has:
Light source control unit, its one end is connected with this central control module; And
At least one light source, each light source is connected in the other end of this light source control unit, and is coupled to this one of them signal window, and launches a light to this fluorescence measurement cavity.
8. fluorescence volume examining system as claimed in claim 7, wherein this each light source be a pulse type laser or a light emitting diode one of them.
9. fluorescence volume examining system as claimed in claim 6, wherein this each spectrometer has:
Spectroscopy unit; And
At least one sense channel module, wherein each sense channel module has a sensing optical fiber and a sensing probe, the two ends of this sensing optical fiber are connected to this spectroscopy unit and this sensing probe, and this sensing probe is coupled to this one of them signal window, to measure a fluorescence signal, and via this sensing optical fiber, this fluorescence signal is sent to this spectroscopy unit to analyze and to produce a fluorescence information.
10. fluorescence volume examining system as claimed in claim 9, wherein this sensing probe be a charge coupled device or a complementary metal oxide semiconductor (CMOS) one of them.
11. fluorescence volume examining systems as claimed in claim 6, wherein this each signal window is a hole.
12. fluorescence volume examining systems as claimed in claim 6, wherein this interior penetrate face be a curved surface, a concave surface, a convex surface or a plane one of them.
13. fluorescence volume examining systems as claimed in claim 12, wherein this is interiorly penetrated face one of them is coated with by a barium sulphate material, a total reflection material or a perfect diffusion material.
14. fluorescence volume examining systems as claimed in claim 6, wherein this body be an integrating sphere, an integration rod or an integral operation module one of them.
15. 1 kinds of fluorescence method for measurement, it includes the following step:
Provide at least one fluorescence to measure cavity, it has body and on this body, offers the inwall of penetrating face at least one signal window and one and be arranged at this body;
At least one light source control module is provided, and it is launched a light to this at least one fluorescence and measures in cavity; And
At least one spectrometer is provided, and it receives a fluorescence signal, to analyze and to produce a fluorescence information.
16. fluorescence method for measurement as claimed in claim 15, it also comprises provides at least one central control module, to drive this each light source control module.
17. fluorescence method for measurement as claimed in claim 16, wherein this each central control module also drives this each spectrometer.
18. fluorescence method for measurement as claimed in claim 15, it also comprises provides fluorescence more than one to measure cavity modes, and this many fluorescence measures cavity modes and makes this each spectrometer analysis and produce a region-wide fluorescence information mean value and a zones of different fluorescence information numerical value.
19. fluorescence method for measurement as claimed in claim 15, it also comprises provides a single fluorescence to measure cavity modes, this list fluorescence measures the region-wide fluorescence information mean value that cavity modes makes this each spectrometer analysis and produces this one of them fluorescence measurement cavity, and this another fluorescence measures a region-wide fluorescence information mean value of cavity.
20. fluorescence method for measurement as claimed in claim 15, wherein this each light source control module has:
Light source control unit, its one end is connected with this central control module; And
At least one light source, each light source is connected in the other end of this light source control unit, and is coupled to this one of them signal window, and launches a light to this fluorescence measurement cavity.
21. fluorescence method for measurement as claimed in claim 20, wherein this each light source be a pulse type laser or a light emitting diode one of them.
22. fluorescence method for measurement as claimed in claim 15, wherein this each spectrometer has:
Spectroscopy unit; And
At least one sense channel module, wherein each sense channel module has a sensing optical fiber and a sensing probe, the two ends of this sensing optical fiber are connected to this spectroscopy unit and this sensing probe, and this sensing probe is coupled to this one of them signal window, to measure a fluorescence signal, and via this sensing optical fiber, this fluorescence signal is sent to this spectroscopy unit to analyze and to produce a fluorescence information.
23. fluorescence method for measurement as claimed in claim 22, wherein this sensing probe be a charge coupled device or a complementary metal oxide semiconductor (CMOS) one of them.
24. fluorescence method for measurement as claimed in claim 15, wherein each signal window is a hole.
25. fluorescence method for measurement as claimed in claim 15, wherein this interior penetrate face be a curved surface, a concave surface, a convex surface or a plane one of them.
26. fluorescence method for measurement as claimed in claim 25, wherein this is interiorly penetrated face one of them is coated with by a barium sulphate material, a total reflection material or a perfect diffusion material.
27. fluorescence method for measurement as claimed in claim 15, wherein this fluorescence measure cavity be an integrating sphere, an integration rod or an integral operation module one of them.
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| TW101143282A TW201421008A (en) | 2012-11-20 | 2012-11-20 | Chamber, system and method of fluorescence measurement |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106872424A (en) * | 2017-01-09 | 2017-06-20 | 浙江大学 | Phytoplankton original position on-line measuring device based on photosynthetic pigments fluorescence |
| CN107991239A (en) * | 2017-11-27 | 2018-05-04 | 上海泽泉科技股份有限公司 | One kind measurement container, measuring system and measuring method |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2344777Y (en) * | 1998-09-16 | 1999-10-20 | 厦门大学 | Liquid cell for fluorimeter |
| CN1556919A (en) * | 2002-03-29 | 2004-12-22 | 大V电子株式会社 | Fluorescence measuring apparatus |
| CN1910445A (en) * | 2004-01-12 | 2007-02-07 | 国际宝石学院 | Fluorescence measuring device for gemstones |
| JP2009210323A (en) * | 2008-03-03 | 2009-09-17 | Nippon Sheet Glass Co Ltd | Multichannel thermal lens spectrometry system and multichannel thermal lens spectrometry method |
| US20100327182A1 (en) * | 2008-02-14 | 2010-12-30 | Starna Scientific Limited | Fluorescence measurement cell |
| US20120277555A1 (en) * | 2008-06-12 | 2012-11-01 | Paseman Sabrina K | Transmission Fluorometer |
-
2012
- 2012-11-20 TW TW101143282A patent/TW201421008A/en unknown
-
2013
- 2013-01-06 CN CN201310003586.2A patent/CN103837510A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2344777Y (en) * | 1998-09-16 | 1999-10-20 | 厦门大学 | Liquid cell for fluorimeter |
| CN1556919A (en) * | 2002-03-29 | 2004-12-22 | 大V电子株式会社 | Fluorescence measuring apparatus |
| CN1910445A (en) * | 2004-01-12 | 2007-02-07 | 国际宝石学院 | Fluorescence measuring device for gemstones |
| US20100327182A1 (en) * | 2008-02-14 | 2010-12-30 | Starna Scientific Limited | Fluorescence measurement cell |
| JP2009210323A (en) * | 2008-03-03 | 2009-09-17 | Nippon Sheet Glass Co Ltd | Multichannel thermal lens spectrometry system and multichannel thermal lens spectrometry method |
| US20120277555A1 (en) * | 2008-06-12 | 2012-11-01 | Paseman Sabrina K | Transmission Fluorometer |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106872424A (en) * | 2017-01-09 | 2017-06-20 | 浙江大学 | Phytoplankton original position on-line measuring device based on photosynthetic pigments fluorescence |
| CN106872424B (en) * | 2017-01-09 | 2019-06-18 | 浙江大学 | Phytoplankton original position on-line measuring device based on photosynthetic pigments fluorescence |
| CN107991239A (en) * | 2017-11-27 | 2018-05-04 | 上海泽泉科技股份有限公司 | One kind measurement container, measuring system and measuring method |
Also Published As
| Publication number | Publication date |
|---|---|
| TW201421008A (en) | 2014-06-01 |
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