CN103804066A - Novel organic microorganism bacterial manure and preparation process thereof - Google Patents
Novel organic microorganism bacterial manure and preparation process thereof Download PDFInfo
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- CN103804066A CN103804066A CN201310285235.5A CN201310285235A CN103804066A CN 103804066 A CN103804066 A CN 103804066A CN 201310285235 A CN201310285235 A CN 201310285235A CN 103804066 A CN103804066 A CN 103804066A
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Abstract
The invention relates to a novel organic microorganism bacterial manure. The novel organic microorganism bacterial manure is prepared by mixing and stirring a bacillus velezensis powder, turfy soil, humic acid, micro-element fertilizers and an auxiliary agent according to a certain ratio, pelletizing and drying, wherein the bacillus velezensis powder is separated from virgin forest soils of Qinling mountains. By using the organic microorganism bacterial manure, the problem of environmental pollution caused by chemical fertilizers can be avoided, thus the soil environment is effectively protected; and the prepared organic microorganism bacterial manure is capable of effectively preventing and treating soil-borne diseases, promoting crop growth and improving the quality and the yield of agricultural products.
Description
Technical field
The present invention relates to a kind of New organic microbial fertilizer formula and preparation technology, belong to organic microbial fertilizer field.
Background technology
Along with the abuse of chemical pesticide and each insecticides, environmental problem is more and more serious, has then caused the degeneration of soil erosion and soil, and the agricultural chemicals high residue problem of agricultural-food also becomes the important factor that affects people's life.Therefore, people are in development economy, more and more pay attention to food safety and eubiosis problem, the parent who so just makes the biogenic preparation of high environment avidity in agriculture production more and more be subject to people looks at, particularly huge to the demand of microbial fertilizer and microbial preparation.
At present, international biogenic preparation is generally divided into: animal, plant, microorganism.Wherein microbial pesticide and botanical pesticide have been realized suitability for industrialized production in production practice.Microbial source fertilizer is to utilize the physiologically active substance etc. of microorganism to carry out growing of regulating plant, is of value to plant-growth and output and improves.The bacterium that wherein produces IAA is attached to root system of plant, and when utilizing plant metabolism to produce secretory product, producing the plant hormone such as IAA and GA affects physiological process and the metamorphosis of plant.Be embodied in and can directly promote the elongation of root, thereby increased the chance contacting with nutritive substance in soil; Can improve the content of Endogenous IAA in plant materials; The expression of inducing plant Analysis of Defence Genes Involved, improves the resistance such as plant materials is disease-resistant, drought resisting.At present, studying in the world more is azospirillum, pseudomonas putida and root nodule bacterium.Spaepen in 2008 proves the change effect of root morphology of Brasil diazotrophic spirillum by experiment, shows as IAA that this bacterium produces and significantly promoted the formation of wheat root hair.Pseudomonas putida has not only played promoter action in cymbidium seed symbiotic germination, and can under greenhouse experiment, significantly promote the output of tomato.B.Ali in 2009 etc., from plant rhizosphere, isolate five kinds of bacterium that produce IAA in phyllosphere.Other that have been reported have the bacterium of the product IAA of direct growth-promoting effect to also have to plant: xanthomonas maltophilia, bacillus cereus, dark green trichoderma, micrococcus sp, Staphylococcus etc.The research application of the organic microbial fertilizer that the Wei Laize genus bacillus BC39 that separation obtains from the virgin forest soil of the Qinling Mountains mentioning in the present invention makes on farm crop has no report.
Summary of the invention
The object of the present invention is to provide a kind of organic microbial fertilizer of novel non-pollution; described organic microbial fertilizer because of avoid with chemical fertilizer have environmental protection and public nuisance free, can not be to the feature of environment; thereby soil protection environment effectively; and the organic microbial fertilizer making both can effectively preventing soil biography evil can also promote plant growth, raising quality of agricultural product and quality.
Organic microbial fertilizer of the present invention, wherein said microorganism is from the virgin forest soil of the Qinling Mountains, to separate the Wei Laize bacillus velezensis (under be called genus bacillus BC39) obtaining, the bacterium powder obtaining after processing by fermentation.
Organic microbial fertilizer of the present invention, comprises the raw material of following weight proportion:
Bacillus BC39 bacterium powder: 5%-50%, turfy soil: 20%-50%, humic acids: 20%-70%; Micro-fertilizer: 1%-15%; Auxiliary agent: 0.5%-10%.
Preferably, organic microbial fertilizer of the present invention, its raw material weight per-cent consists of: bacillus BC39 bacterium powder: 10%-40%, turfy soil: 20%-40%, humic acids: 30%-50%; Micro-fertilizer: 5%-10%; Auxiliary agent: 0.5%-5%.
More have the weight percent of choosing to consist of: bacillus BC39 bacterium powder: 20%, turfy soil: 30%, humic acids: 40%; Micro-fertilizer: 8.5%; Auxiliary agent: 1.5%.
In the present invention, described bacillus BC39 bacterium, be from Wei Laize genus bacillus BC39 be the virgin forest soil of the Qinling Mountains, separate to, the standard of the BC39 of genus bacillus described in formula bacterium powder is: BC39 live body >=30,000,000,000/g.
In the present invention, the standard of described turfy soil is: weight in wet base is about 2%, is no more than 5%, organic content>=30%; The standard of described humic acids is: water content is about 2%, is no more than 5%, organic content>=75%; Described micro-fertilizer is containing key acid ammonium, sodium wolframate, zinc sulfate and magnesium sulfate, and described micro-fertile water content is about 1%, is no more than 3%; Described auxiliary agent is xylogen M
9.In above-mentioned each standard, bacterium is as the criterion in weight proportion.
Particularly, the screening formulation of organic microbial fertilizer of the present invention is in table 1:
The production formula of table 1 organic microbial fertilizer wettable powder
Organic microbial fertilizer of the present invention can be prepared by the following method
Bacterial strain suspension preparation for 1 inoculation
The BC39 bacterial strain that 4 ℃ of lower inclined planes are preserved is placed in activation culture 1～2h, then by its streak inoculation on LB solid plate substratum, cultivate 24h.After bacterial strain is fully grown, picking colony, is inoculated in LB liquid nutrient medium and cultivates 48h, for subsequent use
2 bacterium powder preparations
In liquid fermentation tank, pack liquid nutrient medium into, sterilizing is placed on 1～2d under room temperature, pollution-free on inspection after, inoculate above-mentioned bacteria suspension, by after the fermentation liquor pretreatment obtaining, ultrafiltration and concentration fermented liquid, the dry bacterium powder that obtains of spraying.
The preparation of other materials in 3 bacterial manure
Select nontoxic to bacterium powder, water-absorbing-retaining performance is good, particle is tiny and be easily medium carrier with the turfy soil of seed adhesion, prevented from caking, cheapness.By dry turfy soil, pulverizing, sieve, after sterilizing, for subsequent use.
Turfy soil is mixed with humic acids, micro-fertilizer, add auxiliary agent granulation to form particulate state fertilizer.
The preparation of 4 bacterial manure
After the particulate state fertilizer of 2 gained bacterium powder and 3 gained mixes by a certain percentage, be dried, be packaged to be product of the present invention.
Accompanying drawing explanation
Fig. 1 is organic microbial fertilizer invention Production Flow Chart.
Specific embodiment
The present invention further illustrates by following embodiment, but any embodiment or its combination not should be understood to the restriction to scope of the present invention or embodiment.Scope of the present invention is limited by appended claims, and in conjunction with the general general knowledge of this specification sheets and this area, those of ordinary skills can clearly understand claims limited range.Under prerequisite without departing from the spirit and scope of the present invention, those skilled in the art can carry out any modification or change to technical scheme of the present invention, and this modification and change are also contained in scope of the present invention.
Organic microbial fertilizer in the present invention is a kind of with Wei Laize genus bacillus BC39 bacterium powder, turfy soil, humic acids, micro-fertilizer and the auxiliary agent organic microbial fertilizer that mix and blend, granulation, oven dry make according to a certain percentage.Particular content of the present invention has: by multiple production technique, Wei Laize genus bacillus BC39 is made after bacterium powder, be mixed to get a kind of novel organic microbial fertilizer with turfy soil, humic acids, micro-fertilizer and auxiliary agent, use it for after the booth vegetable melons and fruits such as cucumber, capsicum, tomato, watermelon, strawberry, the quality of agricultural-food improves, output increases.
The preparation of embodiment 1 BC39 bacterium powder
Bacterial strain suspension preparation for 1 inoculation
The BC39 bacterial strain that 4 ℃ of lower inclined planes are preserved is placed in activation culture 1～2h at 30 ℃, after by its streak inoculation in LB solid plate substratum (peptone 10g, yeast extract paste 5g, sodium-chlor 5-10g, agar 20g, pH7.0, cumulative volume 1000ml) upper, cultivate 24h at 30 ℃.After bacterial strain is fully grown, picking colony, is inoculated in LB liquid nutrient medium, under 30 ℃, 150rpm, cultivates 48h, for subsequent use
2 bacterium powder preparations
In liquid fermentation tank, pack 1500L/LB liquid nutrient medium into, sterilizing is placed on 1～2d under room temperature, pollution-free on inspection after, inoculate respectively 6% above-mentioned bacteria suspension, under 30 ℃, 150rpm, cultivate 2～3d, by after fermentation liquor pretreatment, ultrafiltration and concentration fermented liquid, the dry bacterium powder that obtains of spraying.
The preparation of other materials in embodiment 2 organic microbial fertilizers
Select nontoxic to bacterium powder, water-absorbing-retaining performance is good, pH value between 6.5～7.0, particle is tiny and be easily medium carrier with the turfy soil of seed adhesion, prevented from caking, cheapness.By dry turfy soil, pulverize, sieve with 100 sieve apertures, at 100～121 ℃, continuous sterilization is after 30～40 minutes, for subsequent use.
Turfy soil is mixed with humic acids, micro-fertilizer, add auxiliary agent granulation to form particulate state fertilizer.
The preparation of embodiment 3 organic microbial fertilizers
Take BC39 bacterium powder that embodiment 1 prepares, select the turfy soil handled well by following weight ratio, and humic acids, micro-fertilizer, auxiliary agent.
The bacterial manure that table 2 organic microbial fertilizer raw material of the present invention Different Weight proportioning is made
After the particulate state fertilizer of bacterium powder and 3 gained is mixed, be dried, be packaged to be product of the present invention.
Embodiment 4: organic microbial fertilizer of the present invention is to the disease-resistant of different plants and the effect of promoting production
Below experiment is carried out field experiment with the fertilizer of prescription proportioning D gained in the embodiment of the present invention 3; selected prescription D; should not be construed prescription used while being restriction field experiment of the present invention; those skilled in the art, according to the claims in the present invention book and specification sheets scope of disclosure, can easily expect; also can be after the same method; one different prescriptions carry out field experiment, and these, all within protection scope of the present invention.
New organic microbial fertilizer in the present invention is carried out to field test: its to the disease-resistant and effect of increasing production of different plants in table 3 to table 6.
The comparison of several doses of control cucumber fusarium axysporum field efficacies of table 3
From table 3, result can be found out, apply organic microbial fertilizer of the present invention, its diseased plant rate and disease index are low, can effectively prevent the generation of cucumber disease, effect is better than methyl Bu Tuojin, Vetstrep and dislikes mould spirit, compared with clear water control group, difference has significance (P<0.01).
Several chemical control capsicum epidemic disease of table 4 field control effectiveness test result
Several medicaments of table 5 are to bacterial wilt of tomato field test results
Several medicaments of table 6 are to strawberry blight field test results
* in table, each data are the mean value repeating for 3 times.
Result from table 4 to table 6 is known, organic microbial fertilizer of the present invention has disease prevention effect to different plants, can effectively prevent that the disease of plant from sending out rate, and can Promoting plant growth, improve the output of agricultural-food, it,, in disease prevention and raising output, is better than now conventional chemistry fertilizer or chemical insecticide.
Claims (10)
1. a New organic microbial fertilizer, is characterized in that described microbial-bacterial fertilizer comprises the raw material of following weight proportion:
2. organic microbial fertilizer according to claim 1, the weight proportion of wherein said raw material is:
3. organic microbial fertilizer according to claim 2, the weight proportion of wherein said raw material is:
4. according to the organic microbial fertilizer described in any claim in claim 1-3, wherein said genus bacillus BC39 bacterium powder, the standard that contains BC39 live body is: BC39 live body >=30,000,000,000/g.
5. according to the organic microbial fertilizer described in any claim in claim 1-3, the standard of wherein said turfy soil is: water content is no more than 5%, organic content>=30%; The standard of described humic acids is: water content is no more than 5%, organic content>=75%; Described micro-fertilizer is containing key acid ammonium, sodium wolframate, zinc sulfate and magnesium sulfate, and described micro-fertile water content is no more than 3%; Described auxiliary agent is xylogen M
9.
6. a method of preparing the organic microbial fertilizer described in any claim in claim 1-5, comprising: to genus bacillus BC39, ferment after processing, obtain bacterium powder; Gained bacterium powder and turfy soil, humic acids, micro-fertilizer and auxiliary agent are carried out to mixture, granulation and oven dry, be prepared into organic microbial fertilizer.
7. method according to claim 6, wherein prepares the organic microbial fertilizer viable count >=6,000,000,000/g of gained, organic content >=39%.
8. the application of organic microbial fertilizer in soil-borne disease control described in any claim in claim 1-5.
9. the application of organic microbial fertilizer in Promoting plant growth or control pathological changes of plant described in any claim in claim 1-5.
10. application according to claim 9, wherein said plant is selected from cucumber, capsicum, watermelon, tomato or strawberry.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN107011911A (en) * | 2017-03-30 | 2017-08-04 | 深圳市铁汉生态环境股份有限公司 | Beach saline land modifying agent, its preparation method and beach saline land modification method |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102391040A (en) * | 2011-07-19 | 2012-03-28 | 杨凌绿力生物工程有限公司 | New organic microbial fertilizer and preparation process thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102391040A (en) * | 2011-07-19 | 2012-03-28 | 杨凌绿力生物工程有限公司 | New organic microbial fertilizer and preparation process thereof |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107011911A (en) * | 2017-03-30 | 2017-08-04 | 深圳市铁汉生态环境股份有限公司 | Beach saline land modifying agent, its preparation method and beach saline land modification method |
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Application publication date: 20140521 |