CN103800410A - Cultivated ginseng ethanol extract with function of improving studying memory - Google Patents
Cultivated ginseng ethanol extract with function of improving studying memory Download PDFInfo
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- CN103800410A CN103800410A CN201410017082.0A CN201410017082A CN103800410A CN 103800410 A CN103800410 A CN 103800410A CN 201410017082 A CN201410017082 A CN 201410017082A CN 103800410 A CN103800410 A CN 103800410A
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- radix ginseng
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Abstract
The invention provides a cultivated ginseng ethanol extract with a function of improving the studying memory, and provides a preparation method of a five-year cultivated ginseng ethanol extract. Pharmacological experiments indicate that the five-year cultivated ginseng ethanol extract has the function of improving the studying memory.
Description
Technical field
The invention discloses a kind of 5 years agricultural climate condition for corn Radix Ginseng ethanol extractions with enhancing learning and memory function, and the preparation method of this ethanol extraction is provided, belong to Chinese materia medica technical field.
Background technology
Radix Ginseng (Panax ginseng C.A.Meyer) is called " king of BAICAO " by people, its active constituents of medicine has saponin, polysaccharide, polypeptide, flavone, volatilization wet goods.Radix Ginseng has effect to nervous system, cardiovascular system, blood system, digestive system, hormonal system, immune system and genitourinary system, and its medical value is known by people.
5 years raw artificial growth Radix Ginsengs of in JIUYUE, 2012 Ministry of Public Health approval are as new resource food.What Radix Ginseng used as food is the former powder of plant and crude extract, and it eats the regulating action of body physiological function be it be unclear that.From the experimental fact of pharmacology aspect, 5 years oral 3 months of agricultural climate condition for corn Radix Ginseng ethanol extractions, have the effect that strengthens learning and memory function to normal body.
Summary of the invention
The invention provides the preparation method of 5 years agricultural climate condition for corn Radix Ginseng ethanol extractions.
The present invention further discloses the enhancing learning and memory function of 5 years agricultural climate condition for corn Radix Ginseng ethanol extractions.
The specific embodiment
Embodiment 1
The preparation method of 5 years agricultural climate condition for corn Radix Ginseng ethanol extractions
Get 5 years agricultural climate condition for corn Radix Ginseng coarse powder 10g, put into conical flask, add 80ml ethanol (70%), reflux, extract, 1.5h extracting solution is crossed two-layer gauze (centre adds one deck absorbent cotton), collects filtrate;
In residue, add 80ml ethanol (70%), the 1.5h that again refluxes, extracting liquid filtering, wrings out residual extracting solution in ginseng slag with gauze, merges twice filtrate;
Extracting solution is concentrated into without alcohol taste at water-bath, dries, weigh, for subsequent use.
After Radix Ginseng coarse powder 10g is concentrated, obtaining dry powder weight is 3.08g, and extraction ratio is 30.8%.Wherein ginsenoside's total content is about 2.33%(crude drug), ginseng polysaccharide's total content is about 0.76%(crude drug).
Test example 1
The impact on adult rat learning and memory of 5 years agricultural climate condition for corn Radix Ginseng ethanol extractions
1 materials and methods
1.1 medicine experiments are originated in Jilin Fusong County with Radix Ginseng (life in 5 years).Radix Ginseng ethanol extract is that 70% alcohol heating reflux extracts Radix Ginseng coarse powder, filter, concentrated, dry to obtain Radix Ginseng extractum, extraction ratio is 30.8%, and wherein total Ginsenosides Content is about 2.33%, and ginseng polysaccharide's content is 0.76%, provided by research and development centre of Changchun University of Traditional Chinese Medicine biotech lab, lot number is 20110331.
1.2 experiment equipment
Morris water maze device Chengdu TME Technology Co., Ltd.
The program control camera bellows Chinese military medicine academy of science medicine of keeping away of DBA-2 type grinds institute
The self-control of big and small rat diving platform device
1.3 laboratory animal Wistar140, male and female half and half, body weight 180-220g.Provided lot number by Jilin University's zooscopy center: SCXK-(Ji) 2007-2003.Animal, in the indoor sub-cage rearing of Special experimental, freely ingests and drinks water.
140 rats are divided at random Normal group by 1.3 experimental techniques, 5 years agricultural climate condition for corn Radix Ginseng ethanol extracts are little, in, heavy dose of (0.25,0.5,1.0g crude drug/kg) group.Each group rat gastric infusion every day 1 time, continuous 3 months.After administration 3 months, carry out behavioristics's experiment, duration of test continues administration.Water maze test carries out 7 days continuously, first 6 days, at four different place of entry of 1,2,3,4 quadrant, measure rat arrive the time (incubation period), the distance of swimming of platform, towards angle and average speed, within the 7th day, remove platform, measure the number of times of rat spanning platform in 2min, in the residence time of platform area, residence time in the quadrant of platform area, the distance of swimming accounts for the percentage ratio, average speed of total distance of swimming and towards angle in the quadrant of platform area.Water maze test finishes latter second day, carries out step down test, records the number of times being shocked by electricity in every rat 5min or is errors number, using this as school grade, again test after 24h, this is memory and keeps test, records the errors number in incubation period and the 5min that jumps off platform for the 1st time.After finishing, keeps away step down test dark test.The 1st day learning training, records every mice and enters in incubation period in darkroom and 5min and be subject to number of shocks (errors number) after 24h.
1.4 statistical method data acquisitions are processed with SPSS10.0 statistical software.Experimental result is with mean ± standard deviation
form represent, adopt Student ' s t-test to carry out group difference comparison, P<0.05 represents that difference has statistical significance.
2 experimental results
2.1 water maze laboratory
With matched group comparison, Radix Ginseng ethanol extract small dose group, within the 7th day, stop residence time, platform stop distance/total distance ratio of distance, platform area to platform, the platform time of staying/total time, other indexs were without significant change than obviously increase (P<0.05).In Radix Ginseng ethanol extract, dosage group stops residence time, platform stop distance/total distance ratio of distance, platform area on the 7th day to platform, the platform time of staying/total time ratio, number of times through platform obviously extends (P<0.05 or P<0.01), and other indexs are without significant change.The heavy dose of group of Radix Ginseng ethanol extract, the 7th day to the platform 2min inner platform time of staying, in residence time/total time of platform area than the trend that has increase, but not statistically significant, other indexs, without significant change, the results are shown in 1-5.
The impact of table 1 Radix Ginseng on Normal Adult Rat water maze incubation period (s)
*with relatively P<0.05 of Normal group
The impact (cm) of table 2 Radix Ginseng on the Normal Adult Rat water maze distance of swimming
*with relatively P<0.05 of Normal group
Table 3 Radix Ginseng on Normal Adult Rat water maze towards the impact at angle (°)
The impact (cm/s) of table 4 Radix Ginseng on Normal Adult Rat water maze average speed
The impact of table 5 Radix Ginseng on the 7th day water maze laboratory of Normal Adult Rat
*with relatively P<0.05 of Normal group,
*p<0.01
2.2 diving tower experiments
With matched group ratio, the errors number of the heavy dose of group of Radix Ginseng ethanol extract first day obviously reduces (P<0.05), and other each group without significant change, in table 6.
The impact of table 6 Radix Ginseng on the experiment of Normal Adult Rat diving tower
2.3 keep away dark experiment
With matched group comparison, Radix Ginseng ethanol extract is little, in, heavy dose of group of errors number of keeping away the dark experiment error latence of second day and second day have no significant change, in table 7.
Table 7 Radix Ginseng is kept away dark impact to Normal Adult Rat
Claims (3)
1. have a plantation Radix Ginseng ethanol extraction that strengthens learning and memory function, it is characterized in that 5 years agricultural climate condition for corn Radix Ginsengs, extracting mode is ethanol extraction.
2. the preparation method of kind planting Radix Ginseng ethanol extraction, comprises the following steps:
Get 5 years agricultural climate condition for corn Radix Ginseng coarse powder 10g, put into conical flask, add 80ml ethanol (70%), reflux, extract, 1.5h extracting solution is crossed two-layer gauze (centre adds one deck absorbent cotton), collects filtrate;
In residue, add 80ml ethanol (70%), the 1.5h that again refluxes, extracting liquid filtering, wrings out residual extracting solution in ginseng slag with gauze, merges twice filtrate;
Extracting solution is concentrated into without alcohol taste at water-bath, dries, weigh, for subsequent use.
After Radix Ginseng coarse powder 10g is concentrated, obtaining dry powder weight is 3.08g, and extraction ratio is 30.8%.Wherein ginsenoside's total content is about 2.33%(crude drug), ginseng polysaccharide's total content is about 0.76%(crude drug).
3. a kind of plantation Radix Ginseng ethanol extraction with enhancing learning and memory function claimed in claim 1 improves the purposes in learning and memory function medicine in preparation.
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| CN201410017082.0A CN103800410A (en) | 2014-01-15 | 2014-01-15 | Cultivated ginseng ethanol extract with function of improving studying memory |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105166896A (en) * | 2015-09-06 | 2015-12-23 | 吉林大学 | Health-care food capable of improving memory in auxiliary manner |
| JP2019218284A (en) * | 2018-06-18 | 2019-12-26 | オンガネジャパン株式会社 | Oral composition for improving juvenile learning ability, and method for improving juvenile learning ability |
-
2014
- 2014-01-15 CN CN201410017082.0A patent/CN103800410A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105166896A (en) * | 2015-09-06 | 2015-12-23 | 吉林大学 | Health-care food capable of improving memory in auxiliary manner |
| JP2019218284A (en) * | 2018-06-18 | 2019-12-26 | オンガネジャパン株式会社 | Oral composition for improving juvenile learning ability, and method for improving juvenile learning ability |
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Application publication date: 20140521 |