CN103798146B - Rapid seedling growing method for two pieces of euphylla of tobacco single plant - Google Patents
Rapid seedling growing method for two pieces of euphylla of tobacco single plant Download PDFInfo
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- CN103798146B CN103798146B CN201410074577.7A CN201410074577A CN103798146B CN 103798146 B CN103798146 B CN 103798146B CN 201410074577 A CN201410074577 A CN 201410074577A CN 103798146 B CN103798146 B CN 103798146B
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Abstract
The invention discloses a rapid seedling growing method for two pieces of euphylla of a tobacco single plant. One tobacco plant with eight pieces of the euphylla can be rapidly and effectively cultured into four tobacco seedlings by virtue of applying a culture condition, as well as a nutritional medium and cefotaxime sodium. Under the normal culture condition, all the tobacco seedlings obtained in normal time can normally bloom and seed, so as to provide a rapid, portable, efficient and reliable seedling growing method for scientific researches. By adopting the method, the four tobacco seedlings with the same genetic background can be obtained in short time by one tobacco plant; a lot of tobacco seeds with the same genetic background can be obtained in short time; the repeatability and reliability of materials can be scientifically researched.
Description
Technical field
The invention belongs to the vegetative propagation technique field of tobacco, be specifically related to one and grow tobacco individual plant 2 true leaf sprouting and rooting methods.
Background technology
In the research process of Tobacco Germplasm Resources innovation, for cultivating single the precious cigarette strain or the precious individual plant of newfound tobacco that obtain, need cultivation more than 4 months, ability output tobacco seed, in very long more than four months, once this individual plant is dead, the loss of this kind of matter will be caused, or accidentally mix in seed harvest, airing process, all can cause the loss of this kind of matter.Preserved in order to ensure new cultivation and newfound fine individual plant, ensure repeatability and the reliability of follow-up test, become many strains very necessary single cigarette strain Fast-propagation.The strain of the cigarette of many strains simultaneously can gather in the crops a large amount of seeds, for further research provides the research material that the genetic background of preciousness is consistent.
In order to achieve the above object, previous the best way is that the way adopting artificial organ to cultivate carries out vegetative propagation, single-plant propagation is become many strains, but artificial organ cultivates the medium needing price higher, operation link is complicated, and need periodically very long incubation, also need equipment and the chemical reagent such as investment high voltage sterilizing device, superclean bench, incubator, hormone.In addition, in tissue culture procedures, first to be sprouted by hormone induction, and then taken root by hormone induction, finally just can be trained cigarette seedling, a lot of artificial alimentation material of this intermediate demand and hormone, be difficult to ensure that the cigarette seedling obtained is consistent with maternal cigarette strain genetic background, do not undergo mutation, and consuming time very long.Therefore, set up the seedling establishment method that a kind of quick, convenient, efficient, reliable tobacco individual plant becomes many strains, have great importance in tobacco scientific research.
Summary of the invention:
The object of the invention is to study a kind of convenient, efficient, reliable tobacco individual plant and become the seedling establishment method of many strains, thus directly band stem 2 true leaves of 1 tobacco plant are multiplied into 1 complete cigarette strain.For achieving the above object, the invention discloses one and to grow tobacco individual plant 2 true leaf sprouting and rooting methods, fast a strain tobacco can be multiplied into 4 strains, thus make up the deficiencies in the prior art.
Method of the present invention, comprises following step:
1) from the top of the cigarette strain of 8 leaf periods, every 2 true leaf band stems cut, and are cut into 4 joints altogether;
2) nutrient matrix of sterilizing is loaded in the flowerpot of sterilizing, and in the nutrient matrix of sterilizing, pour into the aqueous solution containing Cefotaxime Sodium;
Wherein nutrient matrix comprises organic matter, sand and soil, and its mass ratio is preferably 1:1:3; The organic matter used is made up of wheat straw, chicken manure and fused calcium magnesium phosphate material, and its one is specifically composed as follows: 10 kilograms of rotten wheat straw, dried poultrymanure 0.4 kilogram, 0.02 kilogram, fused calcium magnesium phosphate; Wherein in the aqueous solution, the concentration of Cefotaxime Sodium is: 100-300mg/L;
3) prepared by step 1) band stem cigarette joint according to direction of growth transplanting in step 2) flowerpot in;
4) being placed under framework by flowerpot, frame supported, is to provide space.Then mould with printing opacity
Framework is sealed by material film, and object ensures humidity.Then be placed in greenhouse and cultivate.Temperature in greenhouse
For 25-28 DEG C, illumination is solar irradiation natural daylight.
5) cultivate after 10 days and burrow on the plastic film, then cultivate the area plastic foil on framework being removed in 5 days 50%, object allows cigarette seedling adapt to external environment condition;
6) plastic foil is all thrown off after 16 days and is watered by cultivation, then flowerpot is placed in shady spot in greenhouse and cultivates;
7) cultivate under cigarette seedling being put sun-drenched condition when cultivation 18 days, complete tobacco individual plant 2 true leaf sprouting and rooting.
Water the process that cefotaxime na concn is 200mg/L or 250mg/L in nutrient matrix, 4 joints all energy 100% survive, and such 18 true leaf cigarettes strain has been cultivated into 4 cigarette seedlings.
The invention discloses one to grow tobacco individual plant 2 true leaf sprouting and rooting methods, by condition of culture, and the application of nutrient matrix and Cefotaxime Sodium, fast and effectively 18 true leaf cigarettes strain can be cultivated into 4 cigarette seedlings.And under normal condition of culture, the cigarette seedling obtained within the normal time can normally blossom and bear fruit, for scientific research provides quick, convenient, efficient, reliable seedling establishment method, 1 cigarette strain can be made to obtain genetic background 4 strain cigarette seedlings always at short notice, and obtain the consistent seed of a large amount of genetic background at short notice, ensure that experimental study reliability of material.
Embodiment
The present invention selects the cigarette strain of 8 leaf periods as parent, so both ensure that and once can breed the consistent cigarette strain of 4 genetic background, and, utilize the strain of 8 leaf period cigarettes to be in the characteristic in vegetative period, thus ensure the success rate of breeding.In addition, method of the present invention employs the aqueous solution containing Cefotaxime Sodium in reproductive process, suppresses the germ contamination because wound causes, considerably increases the success rate of seedling, the present invention is simultaneously quick, convenient, efficient, reliable, does not need special appointed condition.
Below in conjunction with example, method of the present invention is described in detail.Utilize the tobacco plant of method of the present invention to the large type of flue-cured tobacco, suncured tabacco, Turkish tobaccos and cigar 4 to breed, effect is all good, and concrete grammar and result are described as follows.
Embodiment 1
1) from the top of the Flue-cured Tobacco strain of 8 leaf periods of tobacco flue-cured tobacco cultivars NC89 and K326 cigarette strain (NC89 and K326 kind is provided by Chinese tobacco germplasm resource bank), every 2 true leaf band stems cut, and are cut into 4 joints altogether, breed;
2) then load in the flowerpot of sterilizing by the nutrient matrix of the sterilizing prepared, after wherein nutrient matrix comprises the mixing of 100g organic matter, 100g river sand and 300g ground surface soil, prepared by high-pressure sterilizing pot 121 DEG C of sterilizing 1.5h.The organic matter used is by 10 kilograms of rotten wheat straw (naturally rotting after being stacked by wheat straw to be formed), 0.4 kilogram of dried poultrymanure and 0.02 kilogram of fused calcium magnesium phosphate (P
2o
5content 18%, CaO content 45%, SiO2 content 20%, content of MgO 12%, impurity and moisture 5%) mix.Then in the nutrient matrix of sterilizing, the aqueous solution containing 250mg/L Cefotaxime Sodium is poured into, till irrigating;
3) plant of being prepared by step 1) according to direction of growth transplanting in flowerpot, flowerpot is placed under metal framework, metal framework can adopt: metal framework described in ZL201220273863.2, then use small plastic bag (patent No.: ZL201320124664.X) to be sealed by metal framework, be then placed in greenhouse and cultivate.In greenhouse, temperature is 25-28 DEG C, and illumination is solar irradiation natural daylight.
4) cultivate after 10 days and burrow on film, thus the environment allowing the new plant cultivated adapt to outside plastic foil;
5) area film on metal frame being removed in 15 days 50% is cultivated again; Thus the environment plant newly cultivated adapted to further outside plastic foil;
6) film all thrown off in 16 days and water, flowerpot being placed on greenhouse shady spot and cultivating;
7) within 18 days, cultivate under rearmounted sun-drenched condition in cultivation, water the process of Cefotaxime Sodium (concentration is 200mg/L and 250mg/L) in nutrient matrix, 4 joints all energy 100% survive, and such 1 strain cigarette is just multiplied into 4 strain cigarettes.Under normal tobacco condition of culture, the cigarette strain obtained in normal incubation time can normally be blossomed and beared fruit.
Table 1: the flue-cured tobacco watering variable concentrations Cefotaxime Sodium in nutrient matrix is trained motility rate (%)
Embodiment 2:
1) from the top of the cigarette strain of the Turkish tobaccos tobacco bred Ke Saxi of 8 leaf periods and Pehanorm inferior (Ke Saxi and the inferior kind of Pehanorm are provided by Chinese tobacco germplasm resource bank), every 2 true leaf band stems cut, and are cut into 4 joints altogether, breed;
2) then load in the flowerpot of sterilizing by the nutrient matrix of the sterilizing prepared, after wherein nutrient matrix comprises the mixing of 100g organic matter, 100g river sand and 300g ground surface soil, prepared by high-pressure sterilizing pot 121 DEG C of sterilizing 1.5h.The organic matter used is by 10 kilograms of rotten wheat straw (naturally rotting after being stacked by wheat straw to be formed), 0.4 kilogram of dried poultrymanure and 0.02 kilogram of fused calcium magnesium phosphate (P
2o
5content 18%, CaO content 45%SiO2 content 20%, content of MgO 12%, impurity and moisture 4%) mix.Then in the nutrient matrix of sterilizing, the aqueous solution containing 250mg/L Cefotaxime Sodium is poured into, till irrigating;
3) plant of being prepared by step 1) according to direction of growth transplanting in flowerpot, flowerpot is placed under metal framework, metal framework can adopt: metal framework described in ZL201220273863.2, then use small plastic bag (patent No.: ZL201320124664.X) to be sealed by metal framework, be then placed in greenhouse and cultivate.In greenhouse, temperature is 25-28 DEG C, and illumination is solar irradiation natural daylight.
4) cultivate after 10 days and burrow on film, thus the environment allowing the new plant cultivated adapt to outside plastic foil;
5) area film on metal frame being removed in 15 days 50% is cultivated again; Thus the environment plant newly cultivated adapted to further outside plastic foil;
6) film all thrown off in 16 days and water, flowerpot being placed on greenhouse shady spot and cultivating;
7) within 18 days, cultivate under rearmounted sun-drenched condition in cultivation.Under normal tobacco condition of culture, the cigarette strain obtained in normal incubation time can normally be blossomed and beared fruit.In nutrient matrix, water the process of Cefotaxime Sodium (concentration is 200mg/L and 250mg/L), 4 joints all energy 100% survive, and such 1 strain cigarette is just multiplied into 4 strain cigarettes.
Table 2: the Turkish tobaccos watering variable concentrations Cefotaxime Sodium in nutrient matrix are trained motility rate (%)
Embodiment 3:
1) top of fluffy (the fluffy kind in the pool is provided by Chinese tobacco germplasm resource bank) cigarette strain from the suncured tabacco tobacco bred pool of 8 leaf periods, every 2 true leaf band stems cut, and are cut into 4 joints altogether, breed;
2) then load in the flowerpot of sterilizing by the nutrient matrix of the sterilizing prepared, after wherein nutrient matrix comprises the mixing of 100g organic matter, 100g river sand and 300g ground surface soil, prepared by high-pressure sterilizing pot 121 DEG C of sterilizing 1.5h.The organic matter used is by 10 kilograms of rotten wheat straw (naturally rotting after being stacked by wheat straw to be formed), 0.4 kilogram of dried poultrymanure and 0.02 kilogram of fused calcium magnesium phosphate (P
2o
5content 18%, CaO content 45%SiO2 content 20%, content of MgO 12%, impurity and moisture 4%) mix.Then in the nutrient matrix of sterilizing, the aqueous solution containing 250mg/L Cefotaxime Sodium is poured into, till irrigating;
3) plant of being prepared by step 1) according to direction of growth transplanting in flowerpot, flowerpot is placed under metal framework, metal framework can adopt: metal framework described in ZL201220273863.2, then use small plastic bag (patent No.: ZL201320124664.X) to be sealed by metal framework, be then placed in greenhouse and cultivate.In greenhouse, temperature is 25-28 DEG C, and illumination is solar irradiation natural daylight.
4) cultivate after 10 days and burrow on film, thus the environment allowing the new plant cultivated adapt to outside plastic foil;
5) area film on metal frame being removed in 15 days 50% is cultivated again; Thus the environment plant newly cultivated adapted to further outside plastic foil;
6) film all thrown off in 16 days and water, flowerpot being placed on greenhouse shady spot and cultivating;
7) within 18 days, cultivate under rearmounted sun-drenched condition in cultivation, water the process of Cefotaxime Sodium (concentration is 200mg/L and 250mg/L) in nutrient matrix, 4 joints all energy 100% survive, and such 1 strain cigarette is just multiplied into 4 strain cigarettes.Under normal tobacco condition of culture, the cigarette strain obtained in normal incubation time can normally be blossomed and beared fruit.
Embodiment 4:
1) from the top of cigar tobacco bred Beinhart1000-1 (Beinhart1000-1 kind is provided by Chinese tobacco germplasm resource bank) the cigarette strain of 8 leaf periods, every 2 true leaf band stems cut, and are cut into 4 joints altogether, breed;
2) then load in the flowerpot of sterilizing by the nutrient matrix of the sterilizing prepared, after wherein nutrient matrix comprises the mixing of 100g organic matter, 100g river sand and 300g ground surface soil, prepared by high-pressure sterilizing pot 121 DEG C of sterilizing 1.5h.The organic matter used is by 10 kilograms of rotten wheat straw (naturally rotting after being stacked by wheat straw to be formed), 0.4 kilogram of dried poultrymanure and 0.02 kilogram of fused calcium magnesium phosphate (P
2o
5content 18%, CaO content 45%SiO2 content 20%, content of MgO 12%, impurity and moisture 4%) mix.Then in the nutrient matrix of sterilizing, the aqueous solution containing 250mg/L Cefotaxime Sodium is poured into, till irrigating;
3) plant of being prepared by step 1) according to direction of growth transplanting in flowerpot, flowerpot is placed under metal framework, metal framework can adopt: metal framework described in ZL201220273863.2, then use small plastic bag (patent No.: ZL201320124664.X) to be sealed by metal framework, be then placed in greenhouse and cultivate.In greenhouse, temperature is 25-28 DEG C, and illumination is solar irradiation natural daylight.
4) cultivate after 10 days and burrow on film, thus the environment allowing the new plant cultivated adapt to outside plastic foil;
5) area film on metal frame being removed in 15 days 50% is cultivated again; Thus the environment plant newly cultivated adapted to further outside plastic foil;
6) film all thrown off in 16 days and water, flowerpot being placed on greenhouse shady spot and cultivating;
7) within 18 days, cultivate under rearmounted sun-drenched condition in cultivation, water the process of Cefotaxime Sodium (concentration is 200mg/L and 250mg/L) in nutrient matrix, 4 joints all energy 100% survive, and such 1 strain cigarette is just multiplied into 4 strain cigarettes.Under normal tobacco condition of culture, the cigarette strain obtained in normal incubation time can normally be blossomed and beared fruit.
The above embodiments prove that method of the present invention can effectively be bred cigarette strain, have good application value.
Claims (3)
1. one to grow tobacco individual plant 2 true leaf sprouting and rooting methods, it is characterized in that, described method comprises following step:
1) from the top of the cigarette strain of 8 leaf periods, every 2 true leaf band stems cut, and are cut into 4 joints altogether;
2) nutrient matrix of sterilizing is loaded in the flowerpot of sterilizing, and in the nutrient matrix of sterilizing, pour into the aqueous solution containing Cefotaxime Sodium;
3) by step 1) the band stem cigarette prepared joint according to direction of growth transplanting in step 2) flowerpot in;
4) be placed under framework by flowerpot, then sealed by framework with light-passing plastic film, be then placed in greenhouse and cultivate, in greenhouse, temperature is 25-28 DEG C, and illumination is natural lighting;
5) cultivate after 10 days and burrow on the plastic film, then cultivate the area plastic foil on framework being removed in 5 days 50%;
6) plastic foil is all thrown off after 16 days and is watered by cultivation, then flowerpot is placed in shady spot in greenhouse and cultivates;
7) cultivate under cigarette seedling being put sun-drenched condition when cultivation 18 days, complete tobacco individual plant 2 true leaf sprouting and rooting;
Described nutrient matrix comprises organic matter, sand and soil, and its mass ratio is 1:1:3, and in the aqueous solution, the concentration of Cefotaxime Sodium is 100-300mg/L.
2. the method for claim 1, it is characterized in that described organic matter is made up of wheat straw, chicken manure and fused calcium magnesium phosphate material, mass ratio is 500:20:1.
3. the method for claim 1, is characterized in that the concentration of Cefotaxime Sodium in the described aqueous solution is 200mg/L or 250mg/L.
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| CN103798146B true CN103798146B (en) | 2015-05-06 |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN202663884U (en) * | 2012-06-01 | 2013-01-16 | 中国农业科学院烟草研究所 | Tool for vertically coating film on single plant of field crop |
| CN103053419A (en) * | 2012-12-28 | 2013-04-24 | 四川省烟草技术中心 | Method for screening root media by using tobacco sterile seedlings |
| CN103109745A (en) * | 2013-03-10 | 2013-05-22 | 通化师范学院 | Method for removing tobacco mosaic virus and rapidly cultivating non-toxic seedling in test tube |
| CN203136663U (en) * | 2013-02-27 | 2013-08-21 | 中国农业科学院烟草研究所 | Single crop stereoscopic cover film |
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2014
- 2014-03-02 CN CN201410074577.7A patent/CN103798146B/en not_active Expired - Fee Related
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN202663884U (en) * | 2012-06-01 | 2013-01-16 | 中国农业科学院烟草研究所 | Tool for vertically coating film on single plant of field crop |
| CN103053419A (en) * | 2012-12-28 | 2013-04-24 | 四川省烟草技术中心 | Method for screening root media by using tobacco sterile seedlings |
| CN203136663U (en) * | 2013-02-27 | 2013-08-21 | 中国农业科学院烟草研究所 | Single crop stereoscopic cover film |
| CN103109745A (en) * | 2013-03-10 | 2013-05-22 | 通化师范学院 | Method for removing tobacco mosaic virus and rapidly cultivating non-toxic seedling in test tube |
Non-Patent Citations (1)
| Title |
|---|
| "烟草快速繁殖及培养的研究";何川生等;《云南农业大学学报》;19981231;第13卷(第4期);第392-396页 * |
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