CN103797988B - A kind of store method of herbaceous plant seeds - Google Patents
A kind of store method of herbaceous plant seeds Download PDFInfo
- Publication number
- CN103797988B CN103797988B CN201210448343.5A CN201210448343A CN103797988B CN 103797988 B CN103797988 B CN 103797988B CN 201210448343 A CN201210448343 A CN 201210448343A CN 103797988 B CN103797988 B CN 103797988B
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- seed
- plant seeds
- herbaceous plant
- store method
- fruit
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- 238000000034 method Methods 0.000 title claims abstract description 27
- 238000004321 preservation Methods 0.000 claims abstract description 12
- 235000013399 edible fruits Nutrition 0.000 claims abstract description 10
- 230000010152 pollination Effects 0.000 claims abstract description 10
- 239000012153 distilled water Substances 0.000 claims abstract description 7
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 7
- 238000003306 harvesting Methods 0.000 claims abstract description 5
- 239000012528 membrane Substances 0.000 claims abstract description 5
- 210000002826 placenta Anatomy 0.000 claims abstract description 5
- 238000012216 screening Methods 0.000 claims abstract description 5
- 229910000831 Steel Inorganic materials 0.000 claims abstract description 4
- 239000010959 steel Substances 0.000 claims abstract description 4
- 241000196324 Embryophyta Species 0.000 claims description 20
- 241000692253 Primula merrilliana Species 0.000 claims description 9
- 235000011179 Primula merrilliana Nutrition 0.000 claims description 9
- 230000001954 sterilising effect Effects 0.000 claims description 7
- 230000004345 fruit ripening Effects 0.000 claims description 2
- 238000007654 immersion Methods 0.000 claims description 2
- 230000007226 seed germination Effects 0.000 abstract description 5
- 238000002791 soaking Methods 0.000 abstract description 3
- 230000035784 germination Effects 0.000 description 25
- 238000003860 storage Methods 0.000 description 9
- 238000002474 experimental method Methods 0.000 description 7
- 238000010413 gardening Methods 0.000 description 3
- 108010016634 Seed Storage Proteins Proteins 0.000 description 2
- 238000013461 design Methods 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 241000208476 Primulaceae Species 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000002372 labelling Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 230000035800 maturation Effects 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- 238000009423 ventilation Methods 0.000 description 1
- 230000003442 weekly effect Effects 0.000 description 1
Abstract
The invention discloses the store method of a kind of herbaceous plant seeds, comprise the steps: that (1) pollinates, flower uses fine steel needle picking long column flower pollen to authorize short column chapiter in opening completely latter 5 days, or long column chapiter authorized by picking short column flower pollen；(2) seed collection, after pollination fruit from green become yellowish-brown time ripe, the fruit of harvesting ripe, gently twist into pieces with tweezers, go peel and placenta i.e. to complete screening and the collection of seed；(3) preservation, seed wrapped and is placed in sterilized culture dish by sterilized filter paper, and topping-up removes unnecessary distilled water after soaking 20 minutes, then seals culture dish with sealed membrane, first lucifuge room temperature preservation stored refrigerated after 3 months.Use technical scheme, it is ensured that the long keeping ability of seed, greatly improve seed germination rate.
Description
Technical field
The invention belongs to field of plant cultivation, it is more particularly related to the store method of a kind of herbaceous plant seeds.
Background technology
Primula merrilliana (PrimulamerrillianaSchltr.) is under the jurisdiction of Primulaceae, it it is the peculiar biennial herb plant in south, Anhui Province, because its flower is many and notable, easily cultivation, florescence is long, artificial cultivation condition can open before the lower Spring Festival, can continue until early or mid May always, is the gardening ornamental flower of a kind of great Development volue.But it is low i.e. to sow germination rate after Primula merrilliana seed mid or late April to mid or late May maturation, about 30%, seedling mortality rate of aestivating is up to more than 90%；And after seed collection common store method after 3 months almost all lose sprouting ability, it is the key technology difficult problem in this plant horticultural gardening that seed effectively preserves.The method of prior art routine Seed storage has following several ways: a kind of is to be dried in the shade by the seed of results, be placed in dry, nice and cool, ventilation under conditions of preserve；The second be the seed of results is in low temperature, be dried, air circulation slowly under the conditions of preserve；The third be the seed of results is placed in low temperature, be dried, airtight under conditions of preserve.Said method is all to emphasize to preserve the seed of results under conditions of being suitably dried, however use Primula merrilliana seed that these methods preserve after three months germination rate and the vigor of seed the lowest, it is impossible to meet horticultural gardening needs.
Summary of the invention
Problem to be solved by this invention is to provide the store method of a kind of suitable Primula merrilliana seed, improves the store method of the herbaceous plant seeds of seed germination rate.
To achieve these goals, the technical scheme that the present invention takes is the store method of a kind of herbaceous plant seeds provided, and comprises the steps:
(1) pollination, flower uses fine steel needle picking long column flower pollen to authorize short column chapiter in opening completely latter 5 days, or long column chapiter authorized by picking short column flower pollen；
(2) seed collection, after pollination fruit from green become yellowish-brown time ripe, the fruit of harvesting ripe, gently twist into pieces with tweezers, go peel and placenta i.e. to complete screening and the collection of seed；
(3) preservation, seed wrapped and is placed in sterilized culture dish by sterilized filter paper, and topping-up removes unnecessary distilled water after soaking a period of time, then seal culture dish with sealed membrane, first lucifuge room temperature preservation stored refrigerated after 3 months.
Preferably, herbaceous plant seeds is the seed of Primula merrilliana.
Preferably, 30-40 days fruit maturations after pollination.
Preferably, the distilled water immersion time is 20 minutes.
Preferably, the condition of preservation is for sealing high humidity, and cryopreserved temperature is 4 DEG C.
Preferably, 20min sterilizing it is incubated at filter paper uses 121 DEG C.
Use technical scheme, after pollination fruit from green become yellowish-brown time ripe, the fruit of harvesting ripe, gently twisting into pieces with tweezers, go peel and placenta i.e. to complete screening and the collection of seed, seed is wrapped and is placed in sterilized culture dish by sterilized filter paper, topping-up removes unnecessary distilled water after soaking a period of time, then with sealed membrane, culture dish is sealed, first lucifuge room temperature preservation 3 months, after turn Refrigerator store；The seed collected is the storage carrying out seed under conditions of high humidity, and after 3 months, germination rate is up to 96%, and germinating energy is 95%.After 12 months, germination rate is 72%, and germinating energy is 66%, it is ensured that the long keeping ability of seed, greatly improves seed germination rate.
Accompanying drawing explanation
Content expressed by width accompanying drawing each to this specification and the labelling in figure are briefly described below:
Fig. 1 be a kind of herbaceous plant seeds of the present invention store method the storage of seeds 3 months after germination rate and vigor comparison figure.
Fig. 2 be a kind of herbaceous plant seeds of the present invention store method the storage of seeds 12 months after germination rate and vigor comparison figure.
Detailed description of the invention
Below against accompanying drawing, by the description to embodiment, the detailed description of the invention of the present invention such as mutual alignment and annexation, the effect of each several part and operation principle, the manufacturing process between the involved shape of each component, structure, each several part and is operated with method etc., it is described in further detail, to help those skilled in the art that inventive concept, the technical scheme of the present invention are had more complete, accurate and deep understanding.
Fig. 1 be a kind of herbaceous plant seeds of the present invention store method the storage of seeds 3 months after germination rate and vigor comparison figure；Fig. 2 be a kind of herbaceous plant seeds of the present invention store method the storage of seeds 12 months after germination rate and vigor comparison figure, the store method of a kind of herbaceous plant seeds of the present invention, comprise the steps:
The 2-6 month then, the herbaceous plant seeds of selection is the seed of Primula merrilliana；
(1) pollination, uses fine steel needle picking long column flower pollen to authorize short column chapiter after flower opens completely, or the method that picking short column spends pollen to authorize long column chapiter is pollinated in 5 days；
(2) seed collection, after pollination 30-40 days, fruit from green become yellowish-brown time ripe, the fruit of harvesting ripe, gently twist into pieces with tweezers, go peel and placenta i.e. to complete screening and the collection of seed；
(3) preservation, filter paper is incubated 20min sterilizing at using 121 DEG C, and sterilized filter paper is standby；Storage practice is divided into this method group, comparison 1-3 group, totally 4 kinds of different storage practices, carries out sprouting experiment after preserving 3 months or after 12 months, and sprouting condition is carried out with reference to GB NY/T1656.5-2008.Germination rate statistics is carried out once weekly, seed exposes white radicle and is considered as seed germination more than 3mm, within continuous 1 month, have no that newly-increased seed germination is considered as sprouting and terminates, calculating germination rate by formula germination rate=germination seed number/subject seeds total grain number × 100%, in starting one month by formula germinating energy=experiment, germination number/subject seeds sum × 100% calculates germinating energy.Concrete storage practice and sprouting result are described below:
This method group: take 600, seed, with the filter paper after sterilizing, seed being packetized into 6 bags, quantity is 100/bag, is respectively placed in sterilized culture dish, topping-up soaks, remove unnecessary distilled water after 20 minutes, then with sealed membrane, culture dish is sealed, lucifuge room temperature preservation, after 3 months the most by the end of August, taking 3 bags that is 300 to carry out sprouting experiment, experimental result is as follows: germination rate is up to 96%, and germinating energy is 95%.Another 3 bags turn the environment stored refrigerated of 4 DEG C, carry out sprouting experiment after 9 months, and sprouting condition is carried out with reference to GB NY/T1656.5-2008, statistical result be germination rate be 72%, germinating energy is 66%.
Matched group 1: take 600, seed, be packetized into 6 bags with the filter paper after sterilizing, quantity is 100/bag, is placed at aeration-drying and keeps in Dark Place, and takes 3 bags and carry out sprouting experiment after 3 months, and germination rate is 8%, and germinating energy is 1%, and after 12 months, germination rate is 0%.
Matched group 2: take 600, seed, be packetized into 6 bags with the filter paper after sterilizing, quantity is 100/bag, being placed in and uncovered in sealed bag put the preservation of 4 DEG C of refrigerator, take 3 bags and carry out sprouting experiment after 3 months, germination rate is 30%, germinating energy is 36%, and after 12 months, germination rate is 0%.
Matched group 3: take 600, seed, it is packetized into 6 bags with the filter paper after sterilizing, quantity is 100/bag, it is placed in sealed bag after driving air as far as possible out of and seals, the preservation of 4 DEG C of refrigerator, take 3 bags after 3 months and carry out sprouting experiment, germination rate is 50%, germinating energy is 36%, and after 12 months, germination rate is 36%, and germinating energy is 35%.
The present invention is it is emphasised that carry out the storage of seed under conditions of high humidity, and after 3 months, germination rate is up to 96%, and germinating energy is 95%.After 12 months, germination rate is 72%, and germinating energy is 66%, it is ensured that the long keeping ability of seed, greatly improves the germination rate of seed；And the Primula merrilliana seed that the method for conventional Seed storage preserves after 3 months, the germination rate of seed is all less than 50%, vigor is less than 40%.
Above in conjunction with accompanying drawing, the present invention is exemplarily described; obviously the present invention implements and is not subject to the restrictions described above; if the improvement of the various unsubstantialities that the method design that have employed the present invention is carried out with technical scheme; or the most improved design by the present invention and technical scheme directly apply to other occasion, all within protection scope of the present invention.
Claims (5)
1. the store method of a herbaceous plant seeds, it is characterised in that: comprise the steps:
(1) pollination, flower uses fine steel needle picking long column flower pollen to authorize short column chapiter in opening completely latter 5 days, or long column chapiter authorized by picking short column flower pollen；
(2) seed collection, after pollination fruit from green become yellowish-brown time ripe, the fruit of harvesting ripe, gently twist into pieces with tweezers, go peel and placenta i.e. to complete screening and the collection of seed；
(3) preservation, wraps seed with sterilized filter paper and is placed in sterilized culture dish, after topping-up soaks a period of time, remove unnecessary distilled water, then seal culture dish with sealed membrane, first lucifuge room temperature preservation 3 months, then stored refrigerated；
Described herbaceous plant seeds is the seed of Primula merrilliana.
2. according to the store method of the herbaceous plant seeds described in claim 1, it is characterised in that: 30 ~ 40 days fruit maturations after described pollination.
3. according to the store method of the herbaceous plant seeds described in claim 1, it is characterised in that: the described distilled water immersion time is about 20 minutes.
4. according to the store method of the herbaceous plant seeds described in claim 1, it is characterised in that: described cryopreserved temperature is 4 DEG C.
5. according to the store method of the herbaceous plant seeds described in claim 1, it is characterised in that: described filter paper is incubated 20min sterilizing at using 121 DEG C.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210448343.5A CN103797988B (en) | 2012-11-12 | 2012-11-12 | A kind of store method of herbaceous plant seeds |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210448343.5A CN103797988B (en) | 2012-11-12 | 2012-11-12 | A kind of store method of herbaceous plant seeds |
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| Publication Number | Publication Date |
|---|---|
| CN103797988A CN103797988A (en) | 2014-05-21 |
| CN103797988B true CN103797988B (en) | 2016-08-03 |
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| CN201210448343.5A Expired - Fee Related CN103797988B (en) | 2012-11-12 | 2012-11-12 | A kind of store method of herbaceous plant seeds |
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101112147A (en) * | 2007-08-30 | 2008-01-30 | 福建师范大学 | Safe hoarding method for Chinese bandoline wood seeds |
| CN101213897A (en) * | 2008-01-08 | 2008-07-09 | 上海交通大学 | Fast dormancy breaking method for celery seed |
| CN101816282A (en) * | 2010-04-26 | 2010-09-01 | 天津科润农业科技股份有限公司 | Hybrid round eggplant seed production method |
| CN102283122A (en) * | 2011-07-11 | 2011-12-21 | 镇江瑞繁农艺有限公司 | Dormancy breaking seeding raising method for water lily seeds |
| CN102498783A (en) * | 2011-10-21 | 2012-06-20 | 芜湖东源新农村开发股份有限公司 | Wild cryptotaenia japonica seed dormancy breaking method |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2000236707A (en) * | 1999-02-22 | 2000-09-05 | Ts Shokubutsu Kenkyusho:Kk | Seed storage |
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2012
- 2012-11-12 CN CN201210448343.5A patent/CN103797988B/en not_active Expired - Fee Related
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101112147A (en) * | 2007-08-30 | 2008-01-30 | 福建师范大学 | Safe hoarding method for Chinese bandoline wood seeds |
| CN101213897A (en) * | 2008-01-08 | 2008-07-09 | 上海交通大学 | Fast dormancy breaking method for celery seed |
| CN101816282A (en) * | 2010-04-26 | 2010-09-01 | 天津科润农业科技股份有限公司 | Hybrid round eggplant seed production method |
| CN102283122A (en) * | 2011-07-11 | 2011-12-21 | 镇江瑞繁农艺有限公司 | Dormancy breaking seeding raising method for water lily seeds |
| CN102498783A (en) * | 2011-10-21 | 2012-06-20 | 芜湖东源新农村开发股份有限公司 | Wild cryptotaenia japonica seed dormancy breaking method |
Non-Patent Citations (1)
| Title |
|---|
| 安徽特有植物安徽羽叶报春的繁殖生物学研究;陈明林;《生物多样性》;20071115(第06期);599-607 * |
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| CN103797988A (en) | 2014-05-21 |
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