CN103755470A - Sustained-release corn specific fertilizer produced from aginomoto mother liquor - Google Patents

Abstract

The invention relates to a sustained-release corn specific fertilizer produced from aginomoto mother liquor, which belongs to special fertilizers for rice. The sustained-release corn specific fertilizer is produced by producing gamma-PGA (pteroylglutamic acid) from bacillus licheniformis strains and fermented aginomoto mother liquor, and adding NH<4+>, P and K of a certain ratio aiming at demands of rice growth. The fertilizer has the advantages that due to adoption of the gamma-PGA produced through fermentation, the fertilizer decomposition speed and the leaching loss can be greatly reduced, the fertilizer efficiency is prolonged to be 120 days from 30 days, and the utilization rate is increased by 95% from 30%. By adopting the product provided by the invention, after manuring of 1-2 times can be avoided, soil hardening caused by excessive chemical fertilizer application is prevented, the result of primary experiments in corn lands show that the yield can be increased by 7.22-24.44% when the fertilizer is used, so that the working intensity and the production cost of farmers are greatly lowered.

Description

Utilize sodium glutamate mother liquid to produce the corn fertilizer with slow releasing function

Technical field

The invention belongs to a kind of corn fertilizer, refer in particular to the corn fertilizer that utilizes sodium glutamate mother liquid to produce.

Background technology

China is glutamate production big country, and annual production occupies first of the world, and 1 ton of monosodium glutamate of every production, discharge 20～25 tons of high concentrated organic waste liquids, i.e. sodium glutamate mother liquids.Sodium glutamate mother liquid refer to Gourmet Powder Factory produce in monosodium glutamate process through etc. the waste liquid that discharges after electric separation and Extraction L-glutamic acid.When domestic Gourmet Powder Factory extracts L-glutamic acid from fermented liquid, it is generally not first separating thallus, directly from the fermented liquid that contains thalline and protein and enriched material thereof, extract L-glutamic acid, and domestic increasing monosodium glutamate enterprise gradually developing trend in etc. electric mother liquor no longer through ion exchange column, reclaim residual L-glutamic acid, but directly fully utilize and wastewater treatment after qualified discharge, to reduce production costs, improve comprehensive benefit.Therefore, Deng containing a large amount of protein and other nutritive ingredients in electric mother liquor, it is reported, L-glutamic acid residual in mother liquor is about 1.2%～1.5%, have even up to 2%, account for 20%～25% of fermentation liquid Glutamic Acid, a large amount of thalline that wherein contain are quality protein sources, and crude protein content accounts for 70%～80%.At present Gourmet Powder Factory adopts concentrated, hydrolysis and neutralization, granulation to make composite fertilizer to sodium glutamate mother liquid.

Different crops is different to the three elements nitrogen of fertilizer, phosphorus, potassium demand, if stereotyped, use nitrogen, phosphorus, the potassium of high-content, do not apply fertilizers scientifically, the rational application of fertilizer, to make plant growth bad, and increase production cost, consume the energy, cause the pollution of environment, do not reach volume increase, increase income, the object of synergy, do not utilize sodium glutamate mother liquid to produce the corn fertilizer with slow releasing function at present.

Summary of the invention

The invention provides a kind of corn fertilizer that utilizes sodium glutamate mother liquid to produce to have slow releasing function, the problem that can not be suitable for corn use completely existing when solving with sodium glutamate mother liquid production composite fertilizer.

The technical scheme that the present invention takes is to be obtained by following method:

(1), utilize activated lichem bacillus strain to carry out fermentation culture, in fermentation culture process, first slant strains is carried out to liquid seeds cultivation, then in fermention medium, carries out fermentation culture with 10% inoculum size; Culture condition, shaking culture: 150～250r/min under pH7.0～7.4,30～40 ℃ of temperature, aerobic conditions, in fermenting process, stream adds glucose and sodium glutamate mother liquid, makes the concentration of glucose and L-glutamic acid all maintain 10-15g/L, and fermentation time is 48～72h;

(2), the sodium glutamate mother liquid that contains γ-PGA in step () being concentrated into dry matter content is 40%;

(3), the sodium glutamate mother liquid concentrated solution pH value that contains γ-PGA that obtains of set-up procedure (two), add wherein 1%～3% bicarbonate of ammonia, making its pH value is 6.2～6.4;

(4), in step (three), add containing NH ₄ ⁺, P, K composition, make N account for whole dry-matter total masses 26～30%, P ₂o ₅account for whole dry-matter total masses 12～15%, K ₂o accounts for 13～16% of whole dry-matter total masses, puddles evenly;

(5) spray granulating, dries, and screening is air-dry, cooling, packing warehouse-in.

The present invention selects the lichen bacillus ferments sodium glutamate mother liquid to generate gamma-polyglutamic acid-(γ-PGA), γ-PGA has very large using value at agriculture field, can make the slow-releasing system of fertilizer, in the fermented liquid of sodium glutamate mother liquid, is rich in gamma-polyglutamic acid-, through concentrated, neutralization, add NH again ₄+, P, K granulation make compoiste fertilizer, this fertilizer contains amino acid, residual sugar, N, K, P and the required trace element of multiple corn, can be for corn planting, to improve corn yield.

γ-the PGA of fermentative production in the present invention, can greatly reduce fertilizer decomposition rate and leaching loss, makes fertilizer efficiency period extend to 120 days from 30 days, and utilization ratio brings up to 95% by 30%.Use this patent product to remove from and topdress for l-2 time, prevent many soil compactions that cause to fertilize, the Preliminary experiment results of carrying out corn field shows to use product of the present invention can increase production 7.22%～24.44%, greatly reduces peasant's labour intensity and production cost.

Therefore this patent has solved the single problem of the original kind of factory's fertilizer, has improved value-added content of product, thereby has realized significant economic benefit.

Embodiment

Embodiment 1

(1), utilize activated lichem bacillus strain to carry out fermentation culture, in fermentation culture process, first slant strains is carried out to liquid seeds cultivation, then in fermention medium, carries out fermentation culture with 10% inoculum size; Culture condition, shaking culture: 150r/min under pH7.0,30 ℃ of temperature, aerobic conditions, in fermenting process, stream adds glucose and sodium glutamate mother liquid, makes the concentration of glucose and L-glutamic acid all maintain 10g/L, and fermentation time is 48h;

(2), the sodium glutamate mother liquid that contains γ-PGA in step () being concentrated into dry matter content is 40%;

(3), the sodium glutamate mother liquid concentrated solution pH value that contains γ-PGA that obtains of set-up procedure (two), add wherein 1% bicarbonate of ammonia, making its pH value is 6.2;

(4), in step (three), add containing NH ₄ ⁺, P, K composition, make N account for whole dry-matter total masses 26%, P ₂o ₅account for whole dry-matter total masses 12%, K ₂o accounts for 13% of whole dry-matter total masses, puddles evenly;

(5) spray granulating, dries, and screening is air-dry, cooling, packing warehouse-in.

Embodiment 2

(1), utilize activated lichem bacillus strain to carry out fermentation culture, in fermentation culture process, first slant strains is carried out to liquid seeds cultivation, then in fermention medium, carries out fermentation culture with 10% inoculum size; Culture condition, shaking culture: 200r/min under pH7.2,35 ℃ of temperature, aerobic conditions, in fermenting process, stream adds glucose and sodium glutamate mother liquid, makes the concentration of glucose and L-glutamic acid all maintain 12.5g/L, and fermentation time is 60h;

(2), the sodium glutamate mother liquid that contains γ-PGA in step () being concentrated into dry matter content is 40%;

(3), the sodium glutamate mother liquid concentrated solution pH value that contains γ-PGA that obtains of set-up procedure (two), add wherein 2% bicarbonate of ammonia, making its pH value is 6.3;

(4), in step (three), add containing NH ₄ ⁺, P, K composition, make N account for whole dry-matter total masses 28%, P ₂o ₅account for whole dry-matter total masses 12.5%, K ₂o accounts for 14.5% of whole dry-matter total masses, puddles evenly;

(5) spray granulating, dries, and screening is air-dry, cooling, packing warehouse-in.

Embodiment 3

(1), utilize activated lichem bacillus strain to carry out fermentation culture, in fermentation culture process, first slant strains is carried out to liquid seeds cultivation, then in fermention medium, carries out fermentation culture with 10% inoculum size; Culture condition, shaking culture: 250r/min under pH7.4,40 ℃ of temperature, aerobic conditions, in fermenting process, stream adds glucose and sodium glutamate mother liquid, makes the concentration of glucose and L-glutamic acid all maintain 15g/L, and fermentation time is 72h;

(2), the sodium glutamate mother liquid that contains γ-PGA in step () being concentrated into dry matter content is 40%;

(3), the sodium glutamate mother liquid concentrated solution pH value that contains γ-PGA that obtains of set-up procedure (two), add wherein 3% bicarbonate of ammonia, making its pH value is 6.4;

(4), in step (three), add containing NH ₄ ⁺, P, K composition, make N account for whole dry-matter total masses 30%, P ₂o ₅account for whole dry-matter total masses 15%, K ₂o accounts for 16% of whole dry-matter total masses, puddles evenly;

(5) spray granulating, dries, and screening is air-dry, cooling, packing warehouse-in.

By specific experiment example, further illustrate the unusual effect of product of the present invention to corn growth below

Experimental example 1

1.1 test materials

For the corn variety that studies the corn research centre seed selection of academy of agricultural sciences, Wu Wei Jilin Province interior single 314; The product of this patent embodiment 1 (containing N:26%, P ₂o ₅: 12%, K ₂o:13%); Duplex composite fertilizer (N:16%, P that Shijiazhuang duplex composite fertilizer limited liability company produces ₂o ₅: 16%, K ₂o:8%); The urea that Chang Mount city, Jilin Province fertilizer plant produces (containing N:46%).

1.2 test method

4 treatment group A, B, C, D that product of the present invention is established in test add 1 control group E(table 1) totally 5 treatment group, experimental plot area 26m ², 8 row districts, long 5 meters of row, line-spacing 0.65m.Random district, community group is arranged, and repeats for 3 times.4 treatment group of product of the present invention are made seed manure and are once applied; Control group is made seed manure with duplex composite fertilizer, with urea, topdresses and once applies in the jointing stage.The planting density of corn is 60000 strains/hm ².

The simultaneous test design of table 1 slow-release fertilizer type composite fertilizer

1.3 overviews experimental field

Test site is carried out in test base, academy of agricultural sciences, Jilin Province.Soil is dark chestnut soil, full nitrogen 1.964g/kg; Alkali-hydrolyzable nitrogen 162.89mg/kg; Available phosphorus 20.256mg/kg; Available potassium 147.036mg/kg; PH=6.96, organic 2.2g/kg.Physical features is smooth, preceding crop corn, and the spring is turned over spring ridging, and irrigation and drainage facility is good

2 results and analysis

2.1 the impact of different treatment on Yield

As can be seen from Table 2, from yield component, the spike length of different amounts product treatment of the present invention, row grain number are all high than contrast, and fringe is thick and tassel row number compared with the control, not quite, the bald sharp length of each product treatment of the present invention is significantly less than contrast in impact.

The impact of table 2 different treatment on yield component

Note: in table, data are to repeat mean value 3 times, E group is CK(contrast)

2.2 impacts of different treatment group on corn yield

Different treatment group on the impact of corn yield in Table 3.As can be seen from Table 3, the output of product treatment of the present invention is all higher than the output of CK, and output amplification is 7.22%～24.44%.In each processing of product of the present invention, product consumption of the present invention is minimum, the output that its output is processed apparently higher than other each on the contrary.The results of analysis of variance shows, each process between difference reach conspicuous level.Community mean yield with each processing carries out LSR test, and result shows, between treatment group A and C, D, difference reaches conspicuous level, and between A and E, difference reaches utmost point conspicuous level.Between all the other each processing, without significant difference, wherein CK output is minimum, and it is the highest that the minimum A of product consumption of the present invention processes output, and it is compared difference and reaches utmost point conspicuous level with CK, be optimum handling.

The corn yield of table 3 different treatment

Claims (1)

1. utilize sodium glutamate mother liquid to produce to have a corn fertilizer for slow releasing function, it is characterized in that being obtained by following method:

(1), utilize activated lichem bacillus strain to carry out fermentation culture, in fermentation culture process, first slant strains is carried out to liquid seeds cultivation, then in fermention medium, carries out fermentation culture with 10% inoculum size; Culture condition, shaking culture: 150～250r/min under pH7.0～7.4,30～40 ℃ of temperature, aerobic conditions, in fermenting process, stream adds glucose and sodium glutamate mother liquid, makes the concentration of glucose and L-glutamic acid all maintain 10-15g/L, and fermentation time is 48～72h;

(2), the sodium glutamate mother liquid that contains γ-PGA in step () being concentrated into dry matter content is 40%;

(3), the sodium glutamate mother liquid concentrated solution pH value that contains γ-PGA that obtains of set-up procedure (two), add wherein 1%～3% bicarbonate of ammonia, making its pH value is 6.2～6.4;

(4), in step (three), add containing NH ₄ ⁺, P, K composition, make N account for whole dry-matter total masses 26～30%, P ₂o ₅account for whole dry-matter total masses 12～15%, K ₂o accounts for 13～16% of whole dry-matter total masses, puddles evenly;

(5) spray granulating, dries, and screening is air-dry, cooling, packing warehouse-in.