CN103751316A - Paliurus ramosissimus extract with antifungal activity and preparations and applications thereof - Google Patents
Paliurus ramosissimus extract with antifungal activity and preparations and applications thereof Download PDFInfo
- Publication number
- CN103751316A CN103751316A CN201410023850.3A CN201410023850A CN103751316A CN 103751316 A CN103751316 A CN 103751316A CN 201410023850 A CN201410023850 A CN 201410023850A CN 103751316 A CN103751316 A CN 103751316A
- Authority
- CN
- China
- Prior art keywords
- waistcoat
- seed extract
- sub
- extract
- ethanol
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Medicines Containing Plant Substances (AREA)
- Cosmetics (AREA)
Abstract
The invention discloses a Paliurus ramosissimus extract with antifungal activity and preparations and applications thereof. The preparation method includes the steps of extracting terpenoids, flavonoids, alkaloids, cumarins (including glycosides and monomers of terpenoids, flavonoids, alkaloids and cumarins) and polysaccharides from a whole plant or any part of Paliurus ramosissimus with solvent, collecting extractive solution, concentrating and drying to obtain Paliurus ramosissimus extract; making into tablets, granules, ointment, gel, film, liniment, lotion and spray. The Paliurus ramosissimus extract with antifungal activity and preparations disclosed by the invention are used for preparing antifungal drugs.
Description
Technical field
The present invention relates to field of medicaments, in particular a kind of waistcoat seed extract and all kinds of preparation and application with antifungal activity.
Background technology
Along with increasing of population, increasing the weight of of environmental pollution, by fungus-caused various diseases, as various tinea unguium, tinea etc., becomes the mankind's frequently-occurring disease.This type of disease incidence is high, and according to statistics, the mycotic sickness rate of China is up to 78%.Mycosis is divided into superficial mycosis and the large class of deep mycosis two.Mycotic infection of superficial part is called again tinea, is a kind of obstinate refractory, chronic dermatosis that re-infection rate is higher, accounts for the more than 80% of mycosis total incidence; Deep mycosis is mainly that fungus is invaded subcutaneous tissue, mucosa and internal organs, take candida albicans infection as main.The mycosis course of disease is long, and relapse rate is high, needs patient's long-term prescription.Treat clinically this type of disease and generally adopt the treatment of ointment partial smearing, or oral antibiotic whole body therapeutic.Wherein pure Western medicine has accounted for very large ratio, and Chinese medicine is little.
Chinese medicine has own unique advantage in treatment infected by microbes disease.Chinese medicine effect relaxes, and toxic and side effects is little, and giving consideration to both the incidental and fundamental is suitable for chronic disease or the patient's long-term taking that has a delicate constitution, and also can greatly reduce or delay simultaneously the generation of microorganism drug resistance.Some have the Chinese medicine of antifungic action and have all applied for patent, for example: patent CN102631379A discloses preparation method and the antifungal application thereof of Ramulus et folium taxi cuspidatae activity extract; Patent CN1895331A discloses Herba Euphorbiae Humifusae effective site and effective ingredient thereof and has prepared the purposes of medicine of antifungic action; Similarly also has CN103155958 etc.The potential effect visible, Chinese medicine has aspect antifungic action.
Waistcoat (Paliurus ramosissimus(Lour.) Poir) be machaka, be a kind of Common Medicinal Herbs, it is reported that branch and leaf root flowers and fruits are all medicinal.Nature and flavor hardship, flat, nontoxic.Can invigorate blood circulation except cold, deliver antipyreticly, detumescence, controls traumatic injury and trusted subordinate's pain.Record in < < Chinese medicine voluminous dictionary > > among the people and some local medicine will.
Summary of the invention
Technical problem to be solved by this invention is for the deficiencies in the prior art, and a kind of waistcoat seed extract and all kinds of preparation and application with antifungal activity is provided.
Technical scheme of the present invention is as follows:
A kind of waistcoat seed extract with antifungal activity, its preparation method is: get the sub-Herb plant of waistcoat or arbitrary position wherein, the 1-20 that adds waistcoat sub-volume methanol or ethanol extraction doubly, or by methanol or ethanol extraction further with ethyl acetate or Petroleum ether extraction; Dry after extracting solution is concentrated, make waistcoat seed extract.
Described waistcoat is the sample of fresh goods, lyophilization product or methanol or Ethanol Treatment; Described extracting method is dipping, backflow or percolation; Described drying means is the dry or microwave drying of drying under reduced pressure, lyophilization, spraying.
The described waistcoat seed extract making comprises glycoside and the monomer of terpenoid, flavonoid, alkaloids, Coumarins and terpenoid, flavonoid, alkaloids, Coumarins.
All kinds of preparations of described waistcoat seed extract comprise waistcoat seed extract tablet, granule, ointment, gel, liniment, liniment, lotion and spray.
Described waistcoat seed extract is applied to the preparation of antifungal drug.
The preparation that is applied to antifungal drug of all kinds of preparations of described waistcoat seed extract.
The waistcoat seed extract with antifungal activity provided by the invention and all kinds of preparation are in the application of antifungal activity.Described Chinese medicine extract is the sub-methanol of waistcoat, ethanol, ethyl acetate, petroleum ether or similar solvent extract.Described waistcoat should be fresh goods, lyophilization product, methanol (ethanol) or other organic solvent pretreatment sample, to guarantee the stable of effective ingredient.
The specific embodiment
Below in conjunction with specific embodiment, the present invention is described in detail.Below in conjunction with embodiment, the present invention is described in further detail, but embodiments of the present invention are not limited to this.
The preparation of embodiment waistcoat seed extract
1, the preparation of the sub-ethanol extraction of waistcoat
(1) get the sub-Herb plant of waistcoat (or wherein arbitrary position) fresh goods 1kg, add 95% (volumetric concentration) of 8 times of waistcoat sub-volume after soak with ethanol 1-2 days, leaf, root and stem are pulverized, with 12 times of 95% ethanol extraction of waistcoat sub-volume, flood 3 days again, collect extracting solution, decompression recycling ethanol, concentrated solution lyophilization obtains the sub-ethanol extraction of waistcoat.
(2) get the sub-Herb plant of waistcoat fresh goods (or wherein arbitrary position) 1kg, pulverize, add 95% alcohol reflux of 8 times 3 times of waistcoat sub-volume, collect extracting solution, decompression recycling ethanol, concentrated solution lyophilization obtains the sub-ethanol extraction of waistcoat.
(3) get the sub-Herb plant of waistcoat fresh goods (root, stem, leaf etc.) 1kg, after lyophilization, pulverize (also can pulverize postlyophilization), add 95% alcohol reflux of 10 times 3 times of waistcoat sub-volume, collect extracting solution, decompression recycling ethanol, concentrated solution lyophilization obtains the sub-ethanol extraction of waistcoat.
2, the preparation of the sub-methanolic extract of waistcoat
Get the sub-Herb plant of waistcoat fresh goods 1kg, pulverize, add the methanol eddy of 6 times of waistcoat sub-volume to extract 3 times, collect extracting solution, reclaim under reduced pressure methanol, concentrated solution lyophilization obtains the sub-methanolic extract of waistcoat.
3, the preparation of the sub-ligroin extraction of waistcoat
(1) concentrated solution of the sub-ethanol extraction of waistcoat, with petroleum ether extraction, reclaims petroleum ether dry.
(2) the sub-methanol extract of waistcoat drying sample, adds 10 times of water suspendibles, with petroleum ether extraction, dry after recovery petroleum ether.
(3) the sub-ethanol extraction drying sample of waistcoat, by 10 times of petroleum ether reflux, extract,, reclaims petroleum ether dry.
4, the preparation of the sub-ethyl acetate extract of waistcoat
(1) concentrated solution of the sub-ethanol extraction of waistcoat, with extracting by 10 times of ethyl acetate again after 10 times of petroleum ether extractiones, dry after recovery ethyl acetate.
(2) the sub-methanol extract of waistcoat drying sample, adds 10 times of times of water suspendibles, with extracting by 10 times of ethyl acetate after 10 times of petroleum ether extractiones again.After reclaiming ethyl acetate, be dried.
(3) the sub-ethanol extraction drying sample of waistcoat, extracts 2 times by 6 times of ethyl acetate backflow, after recovery ethyl acetate, is dried.
2, the preparation of the sub-ethyl acetate extract tablet of waistcoat
Get the sub-ethyl acetate extract of 300g waistcoat, after pulverizing, cross 40 mesh sieves, add 100g microcrystalline Cellulose, 57.5g lactose, 20g cross-linking sodium carboxymethyl cellulose, after mixing, evenly spray body volume concentrations is that 95% alcoholic solution is appropriate, and wet method extruding is granulated, cross 24 mesh sieves, after 50 ℃ of oven dry, then add the magnesium stearate of 20g cross-linking sodium carboxymethyl cellulose and 2.5g, after fully mixing, tabletting, obtains the sub-ethyl acetate extract tablet of waistcoat;
3, the preparation of the sub-ethanol extraction granule of waistcoat
Get the sub-ethanol extraction of 1000g waistcoat, after pulverizing, cross 40 mesh sieves, add 4000g Icing Sugar, after mixing, evenly spray body volume concentrations is that 95% alcoholic solution is appropriate, and wet method extruding is granulated, and crosses 24 mesh sieves, after 50 ℃ of oven dry, granulate, obtains the sub-ethanol extraction granule of waistcoat;
4, the preparation of the sub-methanolic extract ointment of waistcoat
Take 115g octadecanol, 115g white vaseline, 70g glyceryl monostearate, heating and melting, obtains oil phase, adds the sub-methanolic extract of 40g waistcoat; Separately get 100g glycerol, 15g sodium lauryl sulphate, 0.01g cysteine hydrochloride, add 650ml water dissolution, obtain water; Be heated to respectively 75 ℃～80 ℃, under stirring, water slowly added to oil phase, then continue to stir 15 minutes, obtain the sub-methanolic extract ointment of waistcoat.
5, the preparation of the sub-ethanol extraction gel of waistcoat
Get 10g carbomer and be sprinkled in 420ml purified water, stirring makes swelling, adds 100ml propylene glycol stirring and dissolving, stirs the lower 18g of dropping triethanolamine, makes gel-type vehicle; Separately get the sub-ethanol extraction of 100g waistcoat and 2g ethyl hydroxybenzoate and be dissolved in 350ml ethanol, under stirring, add in gel-type vehicle, stir evenly and get final product.
6, the preparation of the sub-ethanol extraction liniment of waistcoat
Getting 40g polyvinyl alcohol 124 swells in 400ml purified water; Separately get the sub-ethanol extraction of 100g waistcoat and be dissolved in 400ml ethanol, then add 100ml glycerol, after stirring, slowly add in poly-vinyl alcohol solution, stir evenly rear filtration, then on filter, add ethanol to 1000ml, obtain.
7, the preparation of the sub-Petroleum ether extraction composition liniment of waistcoat
Get the sub-ligroin extraction fine powder of 100g waistcoat and be placed in mortar, add 500ml Oleum Arachidis hypogaeae semen to grind well, more slowly add saturated calcium hydroxide aqueous solution to 1000ml, be ground to even white milk and get final product.
8, the preparation of the sub-ethanol extraction lotion of waistcoat
Get the sub-ethanol extraction fine powder of 100g waistcoat and be placed in mortar, add 50ml glycerol and appropriate purified water, grind to form pasty state, add gradually purified water to full dose to mix and get final product.
Two, the composition Study of the sub-ethanol extraction of waistcoat
The preparation of sample test liquid: treating excess syndrome is executed the sub-ethanol extraction matter sample of the waistcoat 1g in 1 (1), is dissolved in 25ml dehydrated alcohol, centrifugal.Get supernatant 2ml, with 5 times of dehydrated alcohol dilutions, to final concentration be 0.008g/mi.
1, the inspection of triterpenes components is known
(1) L-B reaction
Sample is dissolved in acetic anhydride, adds several of concentrated sulphuric acid-acetic anhydrides (1: 20), can produce Huang → red → purple → change color such as indigo plant, finally fade, show to contain triterpenoid compound.
(2) Kahlenberg reaction
Sample test liquid is put on filter paper, the chloroformic solution (or the saturated chloroformic solution of Butter of antimony .) of spray 20% Antimony pentachloride, dry rear 60-70 ℃ of heating, aobvious blue, show to contain triterpenoid compound.
(3) R-H reaction
Sample test liquid is dropped on filter paper, and extrusive body volume concentrations is 25% trichloroacetic acid alcoholic solution, is heated to 100 ℃, aobvious red, gradually becomes purple, shows to contain triterpenoid compound.
(4) Salkowki reaction
Ethanol extraction matter sample is dissolved in to chloroform, adds after concentrated sulphuric acid, in sulfuric acid layer, present redness or blueness, chloroform layer has green fluorescence to occur, shows to contain triterpenoid compound.
(5) Tschugaeff reaction
Ethanol extraction matter sample is dissolved in glacial acetic acid, adds that chloroacetic chloride number drips and zinc chloride crystallization number, heat, presents pale red or aubergine a little, shows to contain triterpenoid compound.
2, the inspection of flavones ingredient is known
(1) hydrochloric acid-magnesium powder reduction reaction (reduction reaction colour developing)
The ethanol extraction matter sample that takes a morsel is dissolved in 1mL ethanol, adds a little magnesium powder and concentrated hydrochloric acid, and for a moment, observing response color is aubergine, shows to contain flavone compound in vibration.
(2) aluminum chloride reaction (the complex reaction colour developing of metal ion)
With Glass rod, dip ethanol extraction matter sample test liquid and be applied on filter paper, dry up, the aluminum chloride alcoholic solution of spray 1%, dries up observe phenomena.Put aobvious foresythia under uviol lamp, show to contain flavone compound.
(3) ferric chloride reaction (the complex reaction colour developing of metal ion)
With Glass rod, dip sample test liquid and be applied on filter paper, dry up, under uviol lamp, observe after fluorescence, the ferric chloride alcoholic solution of spray 3%, dries up, and occurs skipper fluorescence speckle, then after ammonia cure, changes brown fluorescence speckle into, shows to contain flavone compound.
(4) alkaline reagent colour developing
With Glass rod, dip sample test liquid and be applied on filter paper, after being dried, spraying with sodium hydrate aqueous solution or be exposed in ammonia steam, while observing under fluorescent light, ammonia steam-energy makes sample color dot change glassy yellow into, shows to contain flavone compound.
3, the inspection of alkaloids composition is known
(1) improvement bismuth potassium iodide (Dragendorff) method
1. bismuth subnitrate 0.85g is dissolved in 10ml glacial acetic acid and 40ml water; 2. potassium iodide 8g is dissolved in 20ml water.Test solution 1. with test solution 2. mixed in equal amounts put in brown bottle to preserve and make stock solution.With front, 1ml stock solution, 4ml glacial acetic acid are mixed with 12ml water.Sample test liquid is added in mentioned reagent, be red tan solution, adding distil water one shakes has precipitation to produce, and shows to contain alkaloid compound.
(2) IKI (Wagner) method
Iodine 1g and potassium iodide 10g are dissolved in 50ml water, add 2ml acetic acid, add water to 100ml.Get mentioned reagent appropriate, add the i.e. aobvious sepia of 1ml methanol extraction matter sample test liquid, show to contain alkaloid compound.
(3) silico-tungstic acid (Bertrand) method
5g silico-tungstic acid is dissolved in 100ml water, adds concentrated hydrochloric acid and is adjusted on a small quantity pH=2 left and right.Get mentioned reagent appropriate, add the i.e. aobvious sepia of 1ml methanol extraction matter sample test liquid, show to contain alkaloid compound.
4, the inspection of Coumarins composition is known
(1) hydroxamic acid ferrum reaction
1. a. oxammonium hydrochloride. 20g is dissolved in 50ml water, and with ethanol dilution, to 200ml, cold place preserves; B. potassium hydroxide 50g is dissolved in little water, adds 500ml ethanol.2. iron chloride (FeCl36H2O) 10g is dissolved in 20ml36% hydrochloric acid solution, and the 200ml that adds diethyl ether shakes up, and puts in hermetic container and preserves.During use by test solution 1. a. mix by 1: 2 with 1. b., elimination precipitation, filtrate is put Refrigerator store.With Glass rod, dip ethyl acetate extract sample test liquid and be applied on filter paper, first spray ab mixing test solution, slightly dry, then spray test solution 2., and aobvious red, show to contain coumarin kind compound.
(2) diazo reagent reaction
1. paranitroanilinum 0.35g, is dissolved in concentrated hydrochloric acid 5ml, adds water to 50ml; 2. sodium nitrite 5g, adds water 50ml.Get 1., 2. liquid equivalent is used after mixing in ice-water bath.The Petroleum ether extraction matter sample test liquid that takes a morsel drips diazo reagent, aobvious orange red, shows to contain coumarin kind compound.
Three, the antifungal activity of waistcoat seed extract research
Take the sub-ethanol extraction 1g of waistcoat of preparation in example 1 (1), with 5% aqueous isopropanol, dissolve and prepare to 10ml, 0.22 μ m membrane filtration degerming.Get the above-mentioned solution of 1ml degerming, join in the PDA culture medium that is cooled to approximately 50 ℃ after 9ml melting, fully shake up, pour into rapidly in the culture dish that diameter is 6cm, standing, make the PDA culture plate that contains 10mg/ml waistcoat seed extract.Make blank PDA culture plate with 5% aqueous isopropanol of same volume.
The truffle inoculum cutting is moved into above-mentioned pastille PDA culture plate, and 25 ℃ of constant temperature culture, when matched group bacterium colony approaches culture dish edge, measure the colony diameter on all culture plates by decussation method, after proofreading and correct, calculate bacteriostasis rate.
Result is as shown in table 1.The sub-ethanol extract of waistcoat has obvious inhibitory action to tested all kinds of funguses.Because tested fungus is common pathomycete, there is obvious representativeness, the sub-ethanol extract of this results suggest waistcoat has stronger antifungal activity, is expected to be applied to the preparation of antimycotic medicine.
The inhibition of the sub-ethanol extract of table 1 waistcoat to various funguses
| Strain | Bacteriostasis rate (%) |
| Candida albicans | 87.53±8.36 |
| Trichophyton | 80.46±10.24 |
| Alpha fungus | 72.69±11.53 |
| Trichophyton violaceum | 90.16±14.36 |
| Trichophyton | 54.39±8.67 |
| Trichophyton verrucosum | 42.63±12.81 |
| Microsporum nanum | 60.54±10.33 |
| Sabouraudites lanosus | 61.32±7.65 |
| Sporothrix schenckii | 76.87±15.69 |
| Phialophora compactum | 66.63±17.65 |
| Exophiala dermatitides | 62.65±8.87 |
| Hormodendrum pedrosei | 53.13±10.08 |
Should be understood that, for those of ordinary skills, can be improved according to the above description or convert, and all these improvement and conversion all should belong to the protection domain of claims of the present invention.
Claims (6)
1. one kind has the waistcoat seed extract of antifungal activity, it is characterized in that, its preparation method is: get the sub-Herb plant of waistcoat or arbitrary position wherein, the 1-20 that adds waistcoat sub-volume methanol or ethanol extraction doubly, or by methanol or ethanol extraction further with ethyl acetate or Petroleum ether extraction; Dry after extracting solution is concentrated, make waistcoat seed extract.
2. waistcoat seed extract according to claim 1, is characterized in that, described waistcoat is the sample of fresh goods, lyophilization product or methanol or Ethanol Treatment; Described extracting method is dipping, backflow or percolation; Described drying means is the dry or microwave drying of drying under reduced pressure, lyophilization, spraying.
3. waistcoat seed extract according to claim 1, is characterized in that, described in the waistcoat seed extract that makes comprise glycoside and the monomer of terpenoid, flavonoid, alkaloids, Coumarins and terpenoid, flavonoid, alkaloids, Coumarins.
4. all kinds of preparations of waistcoat seed extract according to claim 1, is characterized in that, comprise waistcoat seed extract tablet, granule, ointment, gel, liniment, liniment, lotion and spray.
5. the application of waistcoat seed extract according to claim 1, is characterized in that, is used for the treatment of, alleviates fungus-caused clinical disease, the purposes in antifungal drug preparation.
6. the application of all kinds of preparations of waistcoat seed extract according to claim 4, is characterized in that, is used for the treatment of, alleviates fungus-caused clinical disease, the purposes in antifungal drug preparation.
Priority Applications (9)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410023850.3A CN103751316B (en) | 2014-01-17 | 2014-01-17 | A kind of there is antifungal activity waistcoat seed extract and all kinds of preparation and application |
| EP14858822.1A EP3064212B1 (en) | 2013-10-31 | 2014-10-30 | Paliurus ramosissimus (lour.) poir extract and preparation method and uses thereof |
| KR1020167014232A KR101891417B1 (en) | 2013-10-31 | 2014-10-30 | Paliurus ramosissimus(lour.)poir extract and preparation method and uses thereof |
| ES14858822T ES2775598T3 (en) | 2013-10-31 | 2014-10-30 | Paliurus ramosissimus (Lour.) Poir extract and preparation method and uses thereof |
| PCT/CN2014/089895 WO2015062517A1 (en) | 2013-10-31 | 2014-10-30 | Paliurus ramosissimus (lour.) poir extract and preparation method and uses thereof |
| JP2016550924A JP6371400B2 (en) | 2013-10-31 | 2014-10-30 | How to use Hana Natsume, How to use Hana Jujube Extract and How to Use Drug Mixture |
| US15/033,386 US20160256510A1 (en) | 2013-10-31 | 2014-10-30 | Paliurus ramosissimus (lour.) poir extract and preparation method and uses thereof |
| JP2018132095A JP6656316B2 (en) | 2013-10-31 | 2018-07-12 | How to use cucumbers, how to use cucumbers extract and how to use drug mixtures |
| US16/220,229 US10987395B2 (en) | 2013-10-31 | 2018-12-14 | Paliurus ramosissimus (Lour.) Poir. extract and preparation methods and uses thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410023850.3A CN103751316B (en) | 2014-01-17 | 2014-01-17 | A kind of there is antifungal activity waistcoat seed extract and all kinds of preparation and application |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103751316A true CN103751316A (en) | 2014-04-30 |
| CN103751316B CN103751316B (en) | 2016-03-30 |
Family
ID=50518743
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410023850.3A Active CN103751316B (en) | 2013-10-31 | 2014-01-17 | A kind of there is antifungal activity waistcoat seed extract and all kinds of preparation and application |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103751316B (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2015062517A1 (en) * | 2013-10-31 | 2015-05-07 | 四川省中医药科学院 | Paliurus ramosissimus (lour.) poir extract and preparation method and uses thereof |
| CN105796677A (en) * | 2016-05-23 | 2016-07-27 | 四川省中医药科学院 | Application of paliurus ramosissimus (Lour.) poir or extract thereof in preparing medicine for treating infectious ulcer |
| CN105832840A (en) * | 2016-05-23 | 2016-08-10 | 四川省中医药科学院 | Application of paliurus ramosissimus (Lour.) poir or extract thereof to preparation of drug for treating burns and scalds |
| CN105998233A (en) * | 2016-05-23 | 2016-10-12 | 四川省中医药科学院 | Use of paliurus or extract thereof in preparing drug for treating allergic dermatitis |
| CN105998232A (en) * | 2016-05-23 | 2016-10-12 | 四川省中医药科学院 | Use of paliurus or extract thereof in preparing drug for treating acne |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101376870A (en) * | 2008-10-10 | 2009-03-04 | 黄毓星 | Chinese herbal medicine wine capable of activating blood circulation, removing stasis, expelling toxin and relieving itching |
| CN103550323A (en) * | 2013-10-31 | 2014-02-05 | 四川省中医药科学院 | Traditional Chinese medicine extract with antitumor activity as well as preparation method and use thereof |
| CN103735653A (en) * | 2014-01-15 | 2014-04-23 | 四川省中医药科学院 | Traditional Chinese medicine extract with anti-tumor activity as well as preparation method and use thereof |
| CN103751315A (en) * | 2014-01-15 | 2014-04-30 | 四川省中医药科学院 | Traditional Chinese medicine extract capable of resisting fibrosis and preparation method and application thereof |
-
2014
- 2014-01-17 CN CN201410023850.3A patent/CN103751316B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101376870A (en) * | 2008-10-10 | 2009-03-04 | 黄毓星 | Chinese herbal medicine wine capable of activating blood circulation, removing stasis, expelling toxin and relieving itching |
| CN103550323A (en) * | 2013-10-31 | 2014-02-05 | 四川省中医药科学院 | Traditional Chinese medicine extract with antitumor activity as well as preparation method and use thereof |
| CN103735653A (en) * | 2014-01-15 | 2014-04-23 | 四川省中医药科学院 | Traditional Chinese medicine extract with anti-tumor activity as well as preparation method and use thereof |
| CN103751315A (en) * | 2014-01-15 | 2014-04-30 | 四川省中医药科学院 | Traditional Chinese medicine extract capable of resisting fibrosis and preparation method and application thereof |
Non-Patent Citations (1)
| Title |
|---|
| 韦国锋等: "马甲子镇咳祛痰作用及其化学成分的提取分离", 《右江民族医学院学报》 * |
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2015062517A1 (en) * | 2013-10-31 | 2015-05-07 | 四川省中医药科学院 | Paliurus ramosissimus (lour.) poir extract and preparation method and uses thereof |
| US10987395B2 (en) | 2013-10-31 | 2021-04-27 | Sichuan Academy Of Chinese Medicine Sciences | Paliurus ramosissimus (Lour.) Poir. extract and preparation methods and uses thereof |
| CN105796677A (en) * | 2016-05-23 | 2016-07-27 | 四川省中医药科学院 | Application of paliurus ramosissimus (Lour.) poir or extract thereof in preparing medicine for treating infectious ulcer |
| CN105832840A (en) * | 2016-05-23 | 2016-08-10 | 四川省中医药科学院 | Application of paliurus ramosissimus (Lour.) poir or extract thereof to preparation of drug for treating burns and scalds |
| CN105998233A (en) * | 2016-05-23 | 2016-10-12 | 四川省中医药科学院 | Use of paliurus or extract thereof in preparing drug for treating allergic dermatitis |
| CN105998232A (en) * | 2016-05-23 | 2016-10-12 | 四川省中医药科学院 | Use of paliurus or extract thereof in preparing drug for treating acne |
| CN105998232B (en) * | 2016-05-23 | 2019-12-20 | 四川省中医药科学院 | Application of paliurus ramosissimus or extract thereof in preparation of medicine for treating acne |
| CN105832840B (en) * | 2016-05-23 | 2019-12-20 | 四川省中医药科学院 | Application of paliurus ramosissimus or extract thereof in preparation of medicine for treating burns and scalds |
| CN105998233B (en) * | 2016-05-23 | 2020-02-18 | 四川省中医药科学院 | Application of paliurus ramosissimus or extract thereof in preparation of medicine for treating allergic dermatitis |
| CN105796677B (en) * | 2016-05-23 | 2020-03-31 | 四川省中医药科学院 | Application of paliurus ramosissimus or extract thereof in preparation of medicine for treating infectious ulcer |
Also Published As
| Publication number | Publication date |
|---|---|
| CN103751316B (en) | 2016-03-30 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US8632828B2 (en) | Chinese herbal medicine composition used for antiinflammation, detumescence and acesodyne, and preparation method and use thereof | |
| CN103751316B (en) | A kind of there is antifungal activity waistcoat seed extract and all kinds of preparation and application | |
| WO2018058261A1 (en) | Traditional chinese medicine composition for treating psoriasis and preparation method thereof | |
| Kasahara et al. | Effect of methanol extract from flower petals of Tagetes patula L. on acute and chronic inflammation model | |
| CN104857359A (en) | Medicinal composition for treating piglet dysentery and preparation method of medicinal composition | |
| CN103536826B (en) | One is treated colpitic Chinese medicine composition and preparation method thereof | |
| JPH0616530A (en) | Cosmetic | |
| CN103272092A (en) | Huoxiang Zhengqi externally-applied preparation and its preparation method and application | |
| CN102793830A (en) | Pronephrium triphyllum-rheum officinale gel and preparation method thereof | |
| CN108478500A (en) | A kind of composition and its application for oral cavity | |
| CN1318052C (en) | Medicine compositions for treating acne, and its prepn. method | |
| CN110464755B (en) | A Chinese medicinal tablet for treating acute pyelonephritis | |
| CN103800466A (en) | Paliurus ramosissimus extract for treating inflammation or/ and anabrosis of oral cavity and digestive tract as well as preparation and application thereof | |
| Tiwari | Phytochemical analysis and medicinal value of Kalmegh (Andrographis paniculata Nees) | |
| SAMBUDDHA et al. | Research findings of few medicinal plants highlighting the scope for further study | |
| CN104435157B (en) | A kind of Chinese medicine composition for treating lower urinary tract infection and preparation method thereof | |
| CN102188681B (en) | Traditional Chinese drug composition for treating pelvic inflammatory disease, preparation method, application thereof | |
| CN105832840B (en) | Application of paliurus ramosissimus or extract thereof in preparation of medicine for treating burns and scalds | |
| CN105998233B (en) | Application of paliurus ramosissimus or extract thereof in preparation of medicine for treating allergic dermatitis | |
| CN105796677B (en) | Application of paliurus ramosissimus or extract thereof in preparation of medicine for treating infectious ulcer | |
| CN106310063A (en) | Compound gel for treating eczema and preparation method thereof | |
| Raja et al. | Exploration of Mukia maderaspatana as promising topical herbal preparation for cutaneous warts in cattle | |
| CN105998232B (en) | Application of paliurus ramosissimus or extract thereof in preparation of medicine for treating acne | |
| Potaraju et al. | Evaluation of Wound Healing and Anti-inflammatory Activities of Leaves of Ziziphus oenoplia | |
| CN105891401B (en) | Money Hymecromone granular mass control method |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20191009 Address after: 641000 No. 456, Anji street, Neijiang economic and Technological Development Zone, Sichuan Patentee after: Sichuan zitonggong pharmaceutical Limited by Share Ltd Address before: 610041, No. four, 51 South Renmin Road, Chengdu, Sichuan, Wuhou District Patentee before: Sichuan Academy of Chinese Medicine Sciences |