CN105998233B - Application of paliurus ramosissimus or extract thereof in preparation of medicine for treating allergic dermatitis - Google Patents
Application of paliurus ramosissimus or extract thereof in preparation of medicine for treating allergic dermatitis Download PDFInfo
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- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
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- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
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- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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Abstract
The invention belongs to the field of medicines, and relates to a new application of malachite fruit or an extract thereof, in particular to an application of the malachite fruit or the extract thereof in preparing a medicine for treating allergic dermatitis. The externally applied paliurus ramosissimus and the extract thereof can effectively inhibit experimental allergic dermatitis, the onset time and the treatment effect are superior to those of clinical common control drugs, and the result shows that the externally applied paliurus ramosissimus and the extract thereof are superior to the existing common drugs in the treatment effect of treating allergic dermatitis.
Description
Technical Field
The invention belongs to the field of medicines, and relates to a new application of malachite fruit or an extract thereof, in particular to an application of the malachite fruit or the extract thereof in preparing a medicine for treating allergic dermatitis.
Background
The malachite (Paliurus ramosissimus (Lour.) Poir) is deciduous shrub, a common medicinal plant, and is reported to be medicinal for both branches, roots, flowers and fruits. Bitter and mild in nature and taste, and non-toxic. Has effects of dispelling cold, promoting blood circulation, relieving exterior syndrome, relieving fever, and relieving swelling, and can be used for treating traumatic injury and pain of heart and abdomen. However, the use of the paliurus ramosissimus and the extract thereof for treating allergic dermatitis is not reported.
Allergic dermatitis is a skin disease caused by allergens, and mainly refers to skin diseases such as red and swollen skin, itching, wheezy and desquamation and the like caused by contacting human bodies with certain allergens. Specific allergens can be classified into contact allergens, inhalation allergens, ingestion allergens and injection allergens. Each type of allergen can cause corresponding allergic reaction, and the main manifestations are various dermatitis, eczema and urticaria. The topical treatment of allergic dermatitis is extremely important in the whole treatment, and the acute stage is mainly erythema and pimple, and is made into lotion, cream or ointment, such as calamine lotion, shaking lotion, triamcinolone acetonide cream, clofah cream, and skin relaxing cream. The subacute or chronic stage is mainly cream or ointment for external use, and can be corticosteroid ointment, pine distillate ointment, semen Sojae Atricolor distillate ointment, zinc oxide ointment, etc., such as purulent secretion, and antibiotic such as neomycin, erythromycin, bacitracin, or other bactericide such as mupirocin ointment, berberine, mercurial, etc. However, in many patients, these treatments do not alleviate or eliminate symptoms, or recur several days after the onset of the drug, and are in a state of non-response to the drug, which is clinically intractable. At present, the external hormone medicine has definite curative effect on allergic dermatitis, but the side effect and the traditional habit of people limit the use; the traditional Chinese medicine composition has corresponding medicines for infectious ulcer and burn and scald at present, but the effective rate and the cure rate are not high.
The inventor finds that the vest and the extract thereof have good effect of treating the allergic dermatitis in the research process.
Disclosure of Invention
The invention solves the technical problem of providing new application of the vest and the extract thereof.
The new application of the paliurus ramosissimus and the extract thereof provided by the invention is the application of the paliurus ramosissimus or the extract thereof in preparing a medicament for treating allergic dermatitis. Specifically, the medicine is an external medicine.
When in application, the whole plant of the paliurus ramosissimus or a fresh or dry part of any part of the paliurus ramosissimus is adopted as the paliurus ramosissimus; wherein the medicinal part can be any part of root, stem, leaf, flower, fruit or their mixture. The most preferred site is the leaf.
In view of the collection period and the storage convenience of medicinal materials, in order to facilitate clinical medication, the whole plant of the paliurus ramosissimus or a fresh or dried product of any part of the paliurus ramosissimus can be taken as a raw material medicine, and the raw material medicine is directly prepared into a proper preparation for application after being crushed, or can be prepared into an extract for preparation of a proper preparation for application. Specifically, the paliurus ramosissimus is a crushed product or an extract of a fresh or dried product, and the crushed product is prepared by crushing a whole plant of paliurus ramosissimus or a fresh or dried product of any part of the paliurus ramosissimus as a raw material by using a crusher or other apparatuses; the extract is prepared by taking a whole plant of the paliurus ramosissimus or a fresh or dried part of any part of the paliurus ramosissimus as a raw material medicine and adopting a conventional extraction method.
When in use, the paliurus ramosissimus extract is prepared by taking the complete paliurus ramosissimus plant or any part of the paliurus ramosissimus as a raw material medicine and adopting a conventional extraction method. The main components of the paliurus ramosissimus extract comprise flavonoids, terpenoids, alkaloids and coumarins; further comprises the glycosides of the above flavones, terpenes, alkaloids, coumarins, and monomeric components thereof; also comprises polysaccharides and cellulose.
The preparation method of the smashed malachite comprises the following steps:
A. the whole plant of the paliurus ramosissimus or a fresh or dried part of any part of the paliurus ramosissimus is taken as a raw material medicine;
B. pulverizing, and sieving.
The crushing includes crushing by a crusher or other device.
The pulverized product prepared by the above method is called as waistcoat pulverized product.
The preparation method of the vest extract comprises the following steps:
the preparation method comprises the following steps:
the first preparation method of the extract of the paliurus ramosissimus comprises the following steps:
A. the whole plant of the paliurus ramosissimus or a fresh or dried part of any part of the paliurus ramosissimus is taken as a raw material medicine;
B. pulverizing and sieving;
C. extracting with water or organic solvent, and drying.
In the above technical solution, the organic solvent in step C is selected from methanol, ethanol, acetone, isopropanol, ethyl acetate or petroleum ether; ethanol is preferred.
In the technical scheme, the extraction in the step C adopts impregnation, reflux, percolation or other conventional methods for extraction;
in the above technical scheme, the drying in step C is vacuum drying, freeze drying, spray drying, microwave drying or other conventional drying methods.
The second preparation method comprises the following steps:
A. the whole plant of the paliurus ramosissimus or a fresh or dried part of any part of the paliurus ramosissimus is taken as a raw material medicine;
B. pulverizing and sieving;
C. extracting with solvent a, and concentrating the filtrate to obtain concentrated solution;
D. c, extracting the concentrated solution obtained in the step C by using a solvent b to obtain an extract, and drying to obtain the extract;
or, drying the concentrated solution obtained in the step C to obtain an extract 1, carrying out solid-liquid extraction by using a solvent b, and drying to obtain the extract.
In the technical scheme, the extraction method in the step C adopts impregnation, reflux, percolation or other conventional methods for extraction;
in the above technical solution, the solvent a in step C is selected from water and conventional organic solvents (methanol, ethanol, acetone, isopropanol, ethyl acetate or petroleum ether); ethanol is preferred.
In the technical scheme, the solvent b in the step D is selected from ethyl acetate, petroleum ether or n-butanol; ethyl acetate is preferred.
In the technical scheme, the mode that the concentrated solution obtained in the step C is extracted by a solvent b to obtain an extraction liquid belongs to liquid-liquid extraction; and C, drying the concentrated solution obtained in the step C to obtain an extract 1, wherein the extraction mode of the extract by using a solvent b belongs to solid-liquid extraction.
In the above technical scheme, the drying in step D is vacuum drying, freeze drying, spray drying, microwave drying or other conventional drying methods.
The preparation method comprises the following steps:
A. the whole plant of the paliurus ramosissimus or a fresh or dried part of any part of the paliurus ramosissimus is taken as a raw material medicine;
B. extracting with water or organic solvent, and drying.
In the above technical solution, the organic solvent in step B is selected from methanol, ethanol, acetone, isopropanol, ethyl acetate or petroleum ether; ethanol is preferred.
In the technical scheme, the extraction in the step B adopts impregnation, reflux, percolation or other conventional methods for extraction;
in the above technical scheme, the drying in step B is vacuum drying, freeze drying, spray drying, microwave drying or other conventional drying methods.
The preparation method comprises the following steps:
A. the whole plant of the paliurus ramosissimus or a fresh or dried part of any part of the paliurus ramosissimus is taken as a raw material medicine;
B. extracting with solvent a, and concentrating the filtrate to obtain concentrated solution;
C. extracting the filtrate obtained in the step B with a solvent B to obtain a liquid phase, and drying to obtain the compound;
or concentrating and drying the filtrate obtained in the step B to obtain an extract 1, extracting with a solvent B, and drying the extract to obtain the compound.
In the technical scheme, the extraction method in the step B adopts impregnation, reflux, percolation or other conventional methods for extraction;
in the above technical scheme, the solvent a in step B is selected from water and conventional organic solvents (methanol, ethanol, acetone, isopropanol, ethyl acetate or petroleum ether); ethanol is preferred.
In the technical scheme, the solvent b in the step C is selected from ethyl acetate, petroleum ether or n-butanol; ethyl acetate is preferred.
In the technical scheme, the mode that the concentrated solution obtained in the step B is extracted by a solvent B to obtain an extraction liquid belongs to liquid-liquid extraction; and (C) drying the concentrated solution obtained in the step (B) to obtain an extract 1, wherein the extraction mode of the solvent B belongs to solid-liquid extraction.
In the above technical scheme, the drying in step C is vacuum drying, freeze drying, spray drying, microwave drying or other conventional drying methods.
The extracts prepared by the method are named as corresponding extracts by the solvents used in the step C of the method one or the step B of the method three, such as the ethanol extract of the paliurus ramosissimus, the methanol extract of the paliurus ramosissimus, the isopropanol extract of the paliurus ramosissimus, the ethyl acetate extract of the paliurus ramosissimus and the petroleum ether extract of the paliurus ramosissimus. If prepared according to methods two and four, solvent b used in step D of method two or step C of method four is named as the corresponding extract, such as the petroleum ether extract of paliurus ramosissimus, the ethyl acetate extract of paliurus ramosissimus; the extract 1 obtained in the second step D and the fourth step C is named corresponding extract by using the solvent a used in the second step C and the fourth step B, such as the ethanol extract, the methanol extract and the isopropanol extract of the paliurus ramosissimus.
In the technical scheme, the dosage relation of the solvent used in the step C of the first preparation method of the extract and the step B of the third preparation method and the waistcoat is that the adding amount of the solvent is 1-40 times of the weight of the waistcoat; preferably, the addition amount of the solvent is 2-20 times of the weight of the vest; furthermore, the addition amount of the solvent is 4-12 times of the weight of the vest.
In the technical scheme, the dosage relation of the solvent a and the waistcoat used in the step C of the second preparation method and the step B of the fourth preparation method of the extract is that the addition amount of the solvent a is 1-40 times of the weight of the waistcoat; preferably, the addition amount of the solvent a is 2-20 times of the weight of the waistcoat; furthermore, the addition amount of the solvent a is 4-12 times of the weight of the waistcoat.
Preferably, when the solvent a is ethanol, the addition amount of the ethanol is 1-40 times of the mass of the vest; preferably, the addition amount of the ethanol is 2-20 times of the weight of the vest; furthermore, the addition amount of methanol or ethanol is preferably 4-12 times of the weight of the vest.
Wherein the concentration of the ethanol is 10-95%. Preferably, ethanol is used at a concentration of 40-95%. Most preferably, ethanol is used at a concentration of 60-95%.
The medicine is an external preparation. The external preparation is prepared by taking the malachite or the extract thereof as an active ingredient, adding pharmaceutically acceptable auxiliary materials and preparing according to a conventional method; comprises ointment, cream, gel, plastics, liniment, lotion, spray, suspension, solution, emulsion, paste or cataplasm.
Wherein, when the paliurus ramosissimus is taken as an active ingredient, fresh or dry plants are directly crushed, pharmaceutically acceptable auxiliary materials are added, and the external preparation is prepared by a conventional method.
Wherein, when the paliurus ramosissimus extract is taken as an active ingredient, the extract is prepared by the extraction method, pharmaceutically acceptable auxiliary materials are added, and the external preparation is prepared by a conventional method.
In conclusion, the externally applied paliurus ramosissimus and the extract thereof can effectively inhibit experimental allergic dermatitis, the onset time and the treatment effect are superior to those of clinical common control medicaments, and the result shows that the externally applied paliurus ramosissimus and the extract thereof are superior to the existing common medicaments in the treatment effect of treating the allergic dermatitis.
Detailed Description
The following description of specific embodiments of the invention illustrates, but does not limit, the invention.
The technical scheme of the invention is as follows:
pulverizing folium seu cortex Mali Pumilae, stems and leaves of semen Strychni, and whole plant of semen Strychni fresh or dried product, or extracting with water or conventional organic solvent (methanol, ethanol, acetone, isopropanol, ethyl acetate or petroleum ether) 1-40 times of the weight of semen Strychni; or further extracting the extract with ethyl acetate or petroleum ether; concentrating the extractive solution, and drying to obtain semen Strychni extract; the paliurus ramosissimus extract or paliurus ramosissimus raw powder comprises terpenoids, flavonoids, alkaloids, coumarins, polysaccharides and celluloses, and glycosides and monomers of terpenoids, flavones, alkaloids and coumarins.
The paliurus ramosissimus extract or paliurus ramosissimus crude powder is prepared by extracting paliurus ramosissimus with water or conventional organic solvent, or directly pulverizing fresh or dried paliurus ramosissimus into powder; the organic solvent comprises methanol, ethanol, isopropanol, ethyl acetate, and petroleum ether; the extraction method comprises soaking, refluxing, percolating or other conventional extraction methods; the drying method comprises reduced pressure drying, freeze drying, spray drying, microwave drying or other conventional drying methods.
The preparation of the paliurus ramosissimus extract or the paliurus ramosissimus crude powder comprises ointment, cream, gel, plastics, liniment, lotion, spray, suspension, solution, emulsion, paste or cataplasm.
The paliurus ramosissimus extract or paliurus ramosissimus crude drug powder is used for preparing an external medicine for treating allergic dermatitis.
Method for preparing Chinese red waistcoat seed extract or Chinese red waistcoat seed crude drug powder
1. Preparation of water extract of whole plant
Pulverizing 5kg of the whole plant of Strychnos Nus et Schlegel, adding 8 times of water, decocting for 3 times, collecting extractive solution, concentrating under reduced pressure, and drying to obtain water extract of Strychnos Nus et Schlegel.
2. Preparation of whole plant ethanol extract
Pulverizing 5kg of the whole plant of Strychnos Nus-vomica, adding 8 times of 95% ethanol, soaking and extracting for several times, collecting extractive solution, recovering ethanol under reduced pressure until no ethanol smell exists, and drying to obtain Strychnos Nus-vomica ethanol extract.
3. Preparation of ethanol, petroleum ether and ethyl acetate extracts of stem and leaf
Pulverizing stem and leaf of Strychnos Nus-vomica 5kg, adding 10 times of 95% ethanol, reflux-extracting for several times, collecting extractive solution, recovering ethanol under reduced pressure at 60 deg.C until no ethanol smell exists, and drying to obtain Strychnos Nus-vomica ethanol extract. Dispersing the semen Strychni ethanol extract in water, sequentially extracting with petroleum ether and ethyl acetate to obtain semen Strychni petroleum ether extract and ethyl acetate extract.
4. Preparation of extracts of whole plant petroleum ether and ethyl acetate
Pulverizing 5kg of fructus Strychni, adding 10 times of methanol, soaking and extracting for several times, collecting extractive solution, recovering methanol under reduced pressure at 40 deg.C, drying to obtain fructus Strychni methanol extract, dispersing the fructus Strychni methanol extract in water, sequentially extracting with petroleum ether and ethyl acetate to obtain fructus Strychni petroleum ether extract and ethyl acetate extract.
5. 5kg of the whole plant of the paliurus ramosissimus is taken, dried, crushed and sieved by a 80-mesh medicine sieve.
6. Taking 1kg of the whole fresh waistcoat plant, crushing and sieving by a 60-mesh medicine sieve.
Component research of paliurus ramosissimus extract
Qualitative study of component (A)
Preparing a sample test solution: taking 1g of the whole plant ethanol extract sample, dissolving in 25ml of absolute ethanol, and centrifuging. Taking 2ml of supernatant, and diluting with absolute ethyl alcohol by 5 times until the final concentration is 0.008 g/ml.
1. Identification of triterpene compositions
(1) L-B reaction
The sample was dissolved in acetic anhydride and a few drops of concentrated sulfuric acid-acetic anhydride (1: 20) produced a color change of yellow → red → violet → blue, etc., and eventually faded, indicating the presence of triterpenes.
(2) Kahlenberg reaction
Dropping chloroform or alcohol solution of the sample on a filter paper, spraying 20% antimony pentachloride chloroform solution (or antimony trichloride saturated chloroform solution), drying, heating at 60-70 deg.C, and showing blue color, which indicates that the triterpene compound is contained.
(3) R-H reaction
Dropping the sample test solution on filter paper, spraying 25% trichloroacetic acid ethanol solution, heating to 100 deg.C, and gradually changing into purple to indicate that the sample test solution contains triterpenes.
(4) Salkowki reaction
The sample is dissolved in chloroform, and after concentrated sulfuric acid is added, the sulfuric acid layer is red or blue, and the chloroform layer has green fluorescence, which indicates that the sample contains triterpenoids.
(5) Tschugaeff reaction
Dissolving the sample in glacial acetic acid, adding acetyl chloride for a few drops and zinc chloride crystals for a few grains, and slightly heating to show light red or purple red, which indicates that the triterpenes are contained.
2. Identification of flavonoid ingredients
(1) Reduction reaction of hydrochloric acid-magnesium powder (color development reaction)
Dissolving a small amount of sample in 1mL of ethanol, adding a small amount of magnesium powder and concentrated hydrochloric acid, oscillating for a moment, and observing that the reaction color is purple red, which indicates that the product contains flavonoid compounds.
(2) Aluminum trichloride reaction (Complex reaction of Metal ion color development)
Dipping a sample test solution by a glass rod, coating the sample test solution on filter paper, drying, spraying a 1% aluminum trichloride ethanol solution, drying, and observing the phenomenon. The product shows bright yellow under an ultraviolet lamp, which indicates that the product contains flavonoids.
(3) Ferric chloride reaction (Complex reaction of metal ion color development)
Dipping the test solution by a glass rod, coating the test solution on filter paper, drying the filter paper, observing fluorescence under an ultraviolet lamp, spraying a 3% ferric trichloride ethanol solution, drying the filter paper, generating dark blue fluorescent spots, and then converting the dark blue fluorescent spots into brown fluorescent spots after ammonia fumigation, thereby indicating that the filter paper contains flavonoid compounds.
(4) Color development with alkaline reagent
Dipping the test solution with a glass rod, coating the test solution on filter paper, drying, spraying sodium hydroxide aqueous solution or exposing the test solution in ammonia vapor, and observing under a fluorescent lamp, wherein the ammonia vapor can change the color point of the sample into bright yellow, which indicates that the sample contains flavonoid compounds.
3. Detection of alkaloid components
(1) Modified bismuth potassium iodide (Dragendorff) process
① bismuth subnitrate 0.85g dissolved in 10ml glacial acetic acid and 40ml water, ② potassium iodide 8g dissolved in 20ml water, test solution ① and test solution ② equal amount mixed in brown bottle for preservation as stock solution, before use 1ml stock solution, 4ml glacial acetic acid and 12ml water mixed, sample test solution added to the reagent, red brown solution, with distilled water and shaking, precipitation, indicating containing alkaloid compounds.
(2) Iodine-potassium iodide (Wagner) process
Dissolving iodine 1g and potassium iodide 10g in 50ml water, adding acetic acid 2ml, and adding water to 100 ml. And taking a proper amount of the reagent, and adding 1ml of sample test solution to obtain a brown color which shows that the reagent contains alkaloid compounds.
(3) Silicotungstic acid (Bertrand) process
5g of silicotungstic acid is dissolved in 100ml of water, and a small amount of concentrated hydrochloric acid is added to adjust the pH value to about 2. And taking a proper amount of the reagent, and adding 1ml of sample test solution to obtain a brown color which shows that the reagent contains alkaloid compounds.
4. Detection of coumarin compounds
(1) Iron hydroxamate reaction
① a, dissolving 20g of hydroxylamine hydrochloride in 50ml of water, diluting with ethanol to 200ml, storing in a cold place, b, dissolving 50g of potassium hydroxide in a little water, adding 500ml of ethanol, dissolving 10g of ② ferric chloride (FeCl 3.6H2O) in 20ml of 36% hydrochloric acid solution, adding 200ml of diethyl ether, shaking uniformly, storing in a closed container, mixing the test solution ① a and ① b according to a ratio of 1: 2 when in use, filtering out precipitates, storing the filtrate in a refrigerator, dipping a sample by a glass rod, coating the sample on filter paper, spraying the ab mixed test solution firstly, slightly drying, and then spraying the test solution ②, wherein the sample shows red color and contains coumarin compounds.
(2) Diazotization reagent reaction
① g p-nitroaniline, 0.35g, dissolved in 5ml concentrated hydrochloric acid, added with water to 50ml, ② g sodium nitrite, 5g added with water to 50ml, ① and ② liquid are mixed in ice water bath in equal amount for use, a small amount of sample is taken to be tested, and a diazotization reagent is dripped into the sample liquid, thus showing orange red, which indicates that the coumarin compound is contained.
Quantitative analysis of component (II)
1. Taking 0.1g of the extract of the water ethyl acetate of the stem and leaf of the paliurus ramosissimus, adding the water ethyl acetate into a 10ml volumetric flask, adding the water ethyl acetate to the scale, precisely sucking 4ml of the solution from the middle, adding the solution into the 10ml volumetric flask, volatilizing the solvent, adding 0.4ml of 5% vanillin-glacial acetic acid and 1.6ml of perchloric acid, uniformly mixing, diluting the solution to the scale by the water ethyl acetate, heating the solution in a constant-temperature water bath at 70 ℃ for 15min, cooling the solution to the room temperature, transferring the solution to the 10ml volumetric flask, adding the water ethyl acetate to dilute the scale, shaking the solution uniformly, measuring the absorbance at the wavelength of 540nm, and calculating the total triterpene content (the triterpene content is calculated by the American tea acid. The calculated content of triterpenes in 1g of fructus seu radix Kadsurae Oblongifoliae extract is 418 mg.
2. Taking 0.1g of crushed raw hemlock leaf, adding three parts of crushed raw hemlock leaf into a 10ml volumetric flask, adding a proper amount of ethyl acetate, carrying out ultrasonic extraction for 30 minutes, cooling, fixing the volume to a scale, precisely sucking 4ml of solution from the middle part, adding into the 10ml volumetric flask, volatilizing the solvent, adding 0.4ml of 5% vanillin-glacial acetic acid and 1.6ml of perchloric acid, uniformly mixing, diluting to the scale by ethyl acetate, heating in a 70 ℃ constant-temperature water bath for 15min, cooling to room temperature, transferring into the 10ml volumetric flask, adding ethyl acetate to dilute to the scale, shaking uniformly, measuring the absorbance at the wavelength of 540nm, and calculating the total triterpene content (the triterpene content is calculated by the American tea acid) in the test solution. The calculated content of triterpenes in 1g of fructus seu radix Kadsurae Oblongifoliae extract is 46 mg.
3. Taking 0.1g of the crushed parts of the dry parts of the stems and leaves of the paliurus ramosissimus, adding the three parts of the crushed parts into a 10ml volumetric flask, adding a proper amount of ethyl acetate, carrying out ultrasonic extraction for 30 minutes, cooling, fixing the volume to the scale, precisely sucking 4ml of the solution from the middle part, adding the solution into the 10ml volumetric flask, volatilizing the solvent, adding 0.4ml of 5% vanillin-glacial acetic acid and 1.6ml of perchloric acid, uniformly mixing, diluting the solution to the scale by ethyl acetate, heating the solution in a constant-temperature water bath at 70 ℃ for 15min, cooling to the room temperature, transferring the solution into the 10ml volumetric flask, adding the ethyl acetate to the scale, uniformly shaking, measuring the absorbance at a wavelength of 540nm, and calculating the total triterpene content (containing triterpene in terms of the American tea acid) in. The calculated triterpene component in 1g of fructus seu radix Kadsurae Oblongifoliae extract contains 85 mg.
4. Taking 0.1g and three parts of the whole-plant ethanol extract of the paliurus ramosissimus, adding a proper amount of ethyl acetate into a 10ml volumetric flask, ultrasonically extracting for 30 minutes, cooling, fixing the volume to a scale, precisely sucking 4ml of solution from the middle, adding into the 10ml volumetric flask, volatilizing the solvent, adding 0.4ml of 5% vanillin-glacial acetic acid and 1.6ml of perchloric acid, uniformly mixing, diluting to the scale by the ethyl acetate, heating in a 70 ℃ constant-temperature water bath for 15min, cooling to room temperature, transferring into the 10ml volumetric flask, adding the ethyl acetate to dilute to the scale, shaking uniformly, measuring the absorbance at the wavelength of 540nm, and calculating the total triterpene content (containing triterpene in the American tea acid) in the test solution. The calculated content of triterpenes in 1g of fructus seu radix Kadsurae Oblongifoliae extract is 132 mg.
5. Taking 0.1g and three parts of water extract of the whole plant of the paliurus ramosissimus, adding a proper amount of ethyl acetate into a 10ml volumetric flask, carrying out ultrasonic extraction for 30 minutes, cooling, fixing the volume to a scale, precisely sucking 4ml of solution from the middle, adding into the 10ml volumetric flask, volatilizing the solvent, adding 0.4ml of 5% vanillin-glacial acetic acid and 1.6ml of perchloric acid, uniformly mixing, diluting to the scale by the ethyl acetate, heating in a 70 ℃ constant-temperature water bath for 15 minutes, cooling to room temperature, transferring into the 10ml volumetric flask, adding the ethyl acetate to dilute to the scale, shaking uniformly, measuring the absorbance at the wavelength of 540nm, and calculating the total triterpene content (containing triterpene in the American tea acid) in the test solution. The calculated content of triterpenes in 1g of fructus seu radix Kadsurae Oblongifoliae extract is 22 mg.
Preparation of Chinese medicine 'Sanjiazi' preparation
1. Preparation of cream
Taking the extract of the stem leaves of the paliurus ramosissimus with ethyl acetate, and adding appropriate auxiliary materials, such as: making into cream with stearyl alcohol, glyceryl monostearate, glycerol, stearic acid, etc.
Weighing 115g of octadecanol, 115g of white vaseline and 70g of glyceryl monostearate, heating to melt to obtain an oil phase, and adding 40g of the vest ethyl acetate extract; dissolving 100g of glycerol, 15g of sodium dodecyl sulfate and 0.01g of cysteine hydrochloride into 650ml of water to obtain a water phase; respectively heating to 75-80 ℃, slowly adding the water phase into the oil phase under stirring, and then continuously stirring for 15 minutes to obtain the milkvetch seed ethyl acetate extract cream.
2. Preparation of gel of vest seed ethanol extract
Spreading 10g carbomer into 420ml purified water, stirring for swelling, adding 100ml propylene glycol, stirring for dissolving, and dropwise adding 18g triethanolamine under stirring to obtain gel matrix; dissolving 100g of the ethanol extract of semen Strychni and 2g of ethylparaben in 350ml of ethanol, adding into the gel matrix under stirring, and stirring.
3. Preparation of plastics of vest seed ethanol extract
Swelling 40g of polyvinyl alcohol 124 in 400ml of purified water; dissolving 100g of the ethanol extract of the paliurus ramosissimus in 400ml of ethanol, adding 100ml of glycerol, stirring uniformly, slowly adding into the polyvinyl alcohol solution, stirring uniformly, filtering, and adding ethanol from a filter to 1000ml to obtain the composition.
4. Preparation of paliurus ramosissimus petroleum ether extract liniment
Placing 100g of fine powder of the paliurus ramosissimus oil ether extract in a mortar, adding 500ml of peanut oil, uniformly grinding, slowly adding saturated calcium hydroxide aqueous solution to 1000ml, and grinding to obtain a uniform white emulsion.
5. Preparation of water extract lotion of vest
Placing 100g of the fine powder of the water extract of the paliurus ramosissimus into a mortar, adding 50ml of glycerol and a proper amount of purified water, grinding into paste, gradually adding the purified water to the full amount, and uniformly mixing.
6. Preparation of vest seed ethanol extract ointment
Pulverizing the ethanol extract of semen Strychni into fine powder, adding into sterilized and filtered cold liquid paraffin 20g, stirring, adding into dried vaseline 900g and lanolin 50g, stirring, and solidifying to 1000 g.
7. Preparation of a Mali Asiatica paste
Heating and melting 250g of lanolin and 250g of white vaseline in a water bath, and standing at about 60 ℃ for later use; adding 300g of pulverized waistcoat into the above matrix, stirring, adding sieved starch 125g and zinc oxide fine powder 125g, stirring to obtain paste, and cooling.
8. Preparation of cataplasm
200g of sodium polyacrylate, 150g of gelatin and 250g of polyvinyl alcohol are taken as adhesives and dissolved in 100ml of distilled water, and the mixture is heated and stirred until the sodium polyacrylate, the gelatin and the polyvinyl alcohol are completely dissolved and kept stand for standby. Mixing fructus seu radix Kalimeridis ethanol extract 30g, glycerol 350g, and zinc oxide 20g, adding into adhesive, heating and stirring for 10min, standing, degassing, removing bubbles, uniformly coating on 200mm × 25mm non-woven fabric substrate, and standing for gelation.
Fourth, the effect of Mali Asiatica and extract on allergic dermatitis
1. Test method
132 KM mice are divided into 11 groups according to weight layer at random, and the groups are respectively a model control group, a positive control group (Bingpengsan, betamethasone), an ethyl acetate extract small-dose group, an ethyl acetate extract large-dose group, an ethanol extract small-dose group, an ethanol extract large-dose group, a paliurus ramosissimus extract group, a paliurus ramosissimus oil-ether extract group, a paliurus ramosissimus fresh product crushed product group and a paliurus ramosissimus dry product crushed product group. The extracts are all fresh extracts, the concentration is equivalent to 1g fresh product/ml, the same is applied below.
Applying the test substance (extract is applied at a dose of 0.05 ml/animal left ear, and fresh or dry product is applied at a dose of 0.1g fresh/animal left ear) or control substance (Bingpeng powder, 1g crude drug/animal left ear; betamethasone ointment, 10mg crude drug/g ointment, and 0.1g ointment/animal left ear), or solvent 1 time per day for 8 days. The drug was administered to the abdomen of mice at 9d in 25. mu.l of 1% 2, 4-Dinitrofluorobenzene (DNFB) (100 mg DNFB was added to a 1: 1 mixture of acetone and vegetable oil and mixed well, and the volume was adjusted to 10 ml). 1h after the 13d administration, 10. mu.l of 1% DNFB solution was applied to the left ear of the mouse; 24h after smearing, namely 1h after the last administration, the animals are killed by dislocation of cervical vertebrae, ear pieces are weighed, and the swelling and swelling degree of the ears is calculated.
2. Test results
The results are shown in Table 1. The dry and fresh malachite seed and the extract have obvious inhibiting effect on ear swelling, the large dose group of the extract is superior to the small dose group, and the Bingpeng powder has no obvious antiallergic effect.
Table 1 effect of malachite seed and extracts on DNFB-induced ear swelling in mice (n-12,
)
p < 0.05, P < 0.01, compared to model control
3. Conclusion of the experiment
The results are shown in Table 1. The dried and fresh paliurus ramosissimus and the extract can still effectively resist skin anaphylactic reaction caused by DNFB 5 days after the drug is stopped, and the betamethasone and the Bingpeng powder for clinically treating allergic dermatitis have no curative effect after the drug is stopped. The experiment adopts DNFB-stimulated immunoreaction to simulate the abnormal immunoreaction process of the allergic dermatitis, and results show that the dry and fresh vest shells and the extract have a continuous effect on immune skin allergy and have a unique effect on easy relapse of the allergic dermatitis after clinical withdrawal.
Anti-inflammatory action
1. Test method
100 KM mice are divided into 10 groups according to weight layer at random, and the 10 groups are respectively a model control group, a positive control group (cortisone acetate ointment), an ethyl acetate extract small-dose group, an ethyl acetate extract large-dose group, an ethanol extract small-dose group, an ethanol extract large-dose group, a paliurus chinensis petroleum ether extract group, a paliurus chinensis water extract group, a paliurus chinensis fresh product crushed product group and a paliurus chinensis dry product crushed product group. On the day of the experiment, animals were weighed, and the mice were carefully coated on both sides of their right ears with test substances (extract at 0.05 ml/mouse, fresh or dry 0.1g fresh/mouse) or controls (cortisone acetate ointment, 10mg technical/g cream, 0.1g cream/mouse), or solvents. After 40min, the drug is wiped off, xylene (30ul) is carefully smeared on both sides of the right ear of the mouse, the mouse is sacrificed after 20min of modeling, the left and right ears are cut along the base of the auricle of the mouse, the left and right ears are weighed, and the swelling degree (the difference between the two ears) is calculated.
2. Test results
The results are shown in Table 2. Each group of the paliurus ramosissimus extract has obvious inhibition effect on ear swelling caused by xylene stimulation, a large-dose group is superior to a small-dose group, and the effect of a large-dose group of the ethyl acetate extract is similar to that of cortisone acetate.
Table 2 effect of malachite seed and extracts on xylene-induced ear swelling in mice (n-10,
)
p < 0.05, P < 0.01, compared to model control
3. Conclusion of the experiment
The results are shown in table 2: the dry and fresh paliurus ramosissimus and various extracts have definite anti-inflammatory effects on inflammation caused by direct chemical stimulation.
Claims (14)
1. The application of the paliurus ramosissimus extract in preparing the medicine for treating the allergic dermatitis is characterized in that the paliurus ramosissimus extract is an ethyl acetate extract.
2. Use according to claim 1, characterized in that: the paliurus ramosissimus adopts whole paliurus ramosissimus plants or fresh or dry parts of any parts of the paliurus ramosissimus.
3. Use according to claim 2, characterized in that: the part is any one of root, stem, leaf, flower and fruit or their mixture.
4. Use according to claim 2, characterized in that: the part is a leaf.
5. Use according to claim 1, characterized in that: the extract is prepared by taking a whole plant of the paliurus ramosissimus or a fresh or dried product of any part of the paliurus ramosissimus as a raw material medicine and adopting a conventional extraction method.
6. Use according to claim 1, characterized in that: the preparation method of the extract of the paliurus ramosissimus at least comprises the following two methods:
the first preparation method of the extract of the paliurus ramosissimus comprises the following steps:
A. the whole plant of the paliurus ramosissimus or a fresh or dried part of any part of the paliurus ramosissimus is taken as a raw material medicine;
B. pulverizing and sieving;
C. extracting with solvent a, and concentrating the filtrate to obtain concentrated solution;
D. c, extracting the concentrated solution obtained in the step C by using a solvent b to obtain an extract, and drying to obtain the extract;
or, drying the concentrated solution obtained in the step C to obtain an extract 1, performing solid-liquid extraction by using a solvent b, and drying to obtain the extract; the second preparation method of the paliurus ramosissimus extract comprises the following steps:
A. the whole plant of the paliurus ramosissimus or a fresh or dried part of any part of the paliurus ramosissimus is taken as a raw material medicine;
B. extracting with solvent a, and concentrating the filtrate to obtain concentrated solution;
C. extracting the concentrated solution obtained in the step B by using a solvent B to obtain an extract, and drying to obtain the extract;
or, drying the concentrated solution obtained in the step B to obtain an extract 1, performing solid-liquid extraction by using a solvent B, and drying to obtain the extract.
7. Use according to claim 6, characterized in that: any one of the following is satisfied:
in the first preparation method of the extract of the paliurus ramosissimus, the crushing in the step B comprises crushing by a crusher or other devices;
step C in the first preparation method of the extract of the paliurus ramosissimus and step B in the second preparation method of the extract of the paliurus ramosissimus, wherein the extraction method adopts immersion, reflux, percolation or other conventional methods for extraction;
the drying method adopts reduced pressure drying, freeze drying, spray drying or microwave drying;
the step C of the first preparation method of the extract of the paliurus ramosissimus and the step B of the second preparation method of the extract of the paliurus ramosissimus, wherein the solvent a comprises water and an organic solvent; the organic solvent is methanol, ethanol, acetone or isopropanol;
step D of the first preparation method of the paliurus ramosissimus extract and step C of the second preparation method of the paliurus ramosissimus extract, wherein the solvent b is ethyl acetate;
the dosage relation of the solvent a used in the step C of the first preparation method of the extract of the paliurus ramosissimus and the solvent B used in the step B of the second preparation method of the extract of the paliurus ramosissimus is that the addition amount of the solvent a is 1-40 times of the mass of the paliurus ramosissimus;
when the solvent a is ethanol, the concentration of the ethanol is 10-95%.
8. Use according to claim 7, characterized in that: the step C of the first preparation method of the paliurus ramosissimus extract and the step B of the second preparation method of the paliurus ramosissimus extract are that the solvent a comprises water and an organic solvent, and the organic solvent is ethanol.
9. Use according to claim 7, characterized in that: the dosage relationship between the solvent a used in the step C of the first preparation method of the paliurus ramosissimus extract and the solvent B used in the step B of the second preparation method of the paliurus ramosissimus extract is that the addition amount of the solvent a is 2-20 times of the mass of the paliurus ramosissimus.
10. Use according to claim 9, characterized in that: the addition amount of the solvent a is 4-12 times of the weight of the vest.
11. Use according to claim 7, characterized in that: when the solvent a used in the step C of the first preparation method of the extract of the paliurus ramosissimus and the step B of the second preparation method of the extract of the paliurus ramosissimus is ethanol, the concentration of the ethanol is 40-95%.
12. Use according to claim 11, characterized in that: the concentration of the ethanol is 60-95%.
13. Use according to any one of claims 1 to 12, characterized in that: the external preparation is prepared by taking the crushed product or extract of the whole plant of the paliurus ramosissimus or the fresh/dry product of any part of the paliurus ramosissimus as a raw material and adding pharmaceutically acceptable auxiliary materials according to a conventional method.
14. Use according to claim 13, characterized in that: the external preparation comprises ointment, cream, gel, plastics, liniment, lotion, spray, suspension, solution, emulsion, paste or cataplasm.
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Citations (3)
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| CN103690840A (en) * | 2013-12-09 | 2014-04-02 | 赵升瑞 | Pure traditional Chinese medicine formulation for treating allergy and preparation method of pure traditional Chinese medicine formulation |
| CN103751316A (en) * | 2014-01-17 | 2014-04-30 | 四川省中医药科学院 | Paliurus ramosissimus extract with antifungal activity and preparations and applications thereof |
| CN105147875A (en) * | 2015-07-22 | 2015-12-16 | 当涂县科辉商贸有限公司 | External traditional Chinese medicine for treating allergic dermatitis |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103690840A (en) * | 2013-12-09 | 2014-04-02 | 赵升瑞 | Pure traditional Chinese medicine formulation for treating allergy and preparation method of pure traditional Chinese medicine formulation |
| CN103751316A (en) * | 2014-01-17 | 2014-04-30 | 四川省中医药科学院 | Paliurus ramosissimus extract with antifungal activity and preparations and applications thereof |
| CN105147875A (en) * | 2015-07-22 | 2015-12-16 | 当涂县科辉商贸有限公司 | External traditional Chinese medicine for treating allergic dermatitis |
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Effective date of registration: 20200827 Address after: 641000 No. 456, Anji street, Neijiang economic and Technological Development Zone, Sichuan Patentee after: Sichuan zitonggong pharmaceutical Limited by Share Ltd. Address before: 610041, No. four, 51 South Renmin Road, Chengdu, Sichuan, Wuhou District Patentee before: SICHUAN ACADEMY OF CHINESE MEDICINE SCIENCES |