CN103751231A - Application of gingko extract nanometer liposome in acne removing and dermatosis treatment - Google Patents
Application of gingko extract nanometer liposome in acne removing and dermatosis treatment Download PDFInfo
- Publication number
- CN103751231A CN103751231A CN201310409572.0A CN201310409572A CN103751231A CN 103751231 A CN103751231 A CN 103751231A CN 201310409572 A CN201310409572 A CN 201310409572A CN 103751231 A CN103751231 A CN 103751231A
- Authority
- CN
- China
- Prior art keywords
- semen ginkgo
- nanometer liposome
- ginkgo extrac
- preparation
- dermatosis
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Medicinal Preparation (AREA)
- Cosmetics (AREA)
Abstract
The present invention relates to a use of gingko extract nanometer liposome in preparation of external application preparations for acne removing and dermatosis treatment. According to the present invention, the gingko extract can be obtained from extracts of leaf, fruit, exopleura, rhizome and other parts of gingko through a chemical separation method; the gingko extract nanometer liposome is prepared by using a film ultrasound-high pressure homogenization method; the dermatosis is infectious, inflammatory and allergic skin diseases; the drug, the cosmetic or the disinfectant external application preparation containing the gingko extract nanometer liposome and provided for acne removing and dermatosis treatment contains 0.001-80% of the gingko extract nanometer liposome; results of pharmacology studies show that the gingko extract nanometer liposome provides significant anti-common skin pathogen infection effects, anti-inflammatory effects and anti-allergic effects, and the effect of the gingko extract nanometer liposome is significantly superior to the effect of the gingko extract external application preparation control group; and results of clinical trials on infectious or allergic skin disease patients show that the gingko extract nanometer liposome provides significant treatment effects for a variety of skin diseases, wherein the effective rate is 100%, and characteristics of safe use and no side effect are provided.
Description
Technical field
The invention belongs to biological medicine and therapeutic make-up technique field, be specifically related to Semen Ginkgo extrac nanometer liposome in the application in treatment dermatosis and anti-acne.
Background technology
Most dermatosis persistent period are long, and easily outbreak repeatedly, brings misery to patient, has a strong impact on patient's physical and mental health.Dermatitis can be divided into infectious and non-infectious.Infectious skin disease can be caused by antibacterial, virus, fungus and protozoon, as acne, impetigo, folliculitis, herpes, tinea manus and pedis, tinea corporis, acariasis, scabies etc.; Acne can propionibacterium acnes and infection of staphylococcus aureus cause.Non-infectious dermatosis mostly is allergy or anaphylaxis dermatosis, as neurodermatitis, eczema, urticaria, miliaria, skin pruritus etc.Treatment infectious skin disease mostly is antimicrobial drug, antiviral agents or antifungal agent at present; The medicine for the treatment of dermatitis and allergic skin disease mostly is corticosteroid hormone, though these medicines have effect for the moment, often occurs that skin lesion worsens after drug withdrawal; Prolonged application can cause many side effect.For fear of the generation of this type of bad phenomenon, market demand develop a kind of derive from natural, effect significantly, effect extensively, without the dermopathic external preparation for the treatment of of obvious adverse reaction, to alleviate patient suffering.Dermatosis is of a great variety and patient is numerous, and therefore develop this type of and treat dermopathic external preparation product and there is good potential applicability in clinical practice, to patient, be very useful and necessary.
Semen Ginkgo is the medicinal plants of China's preciousness, and leaf, fruit and the episperm etc. of Semen Ginkgo all have medicinal exploitation and are worth.According to < < Compendium of Materia Medica > >, record, Semen Ginkgo property is sweet, bitter, flat, puckery; Have that relieving asthma and cough, leukorrhagia stopping are turbid, the merit of pain relieving; Cure mainly cough of cold type phlegm dyspnea, asthma, frequent micturition, leucorrhea with red and white discharge, rhagadia manus et pedis etc.Particularly it is found that in recent decades in Folium Ginkgo and contain plurality of active ingredients, main effective ingredient is flavonoid and terpene lactone compound, has the function of control cardiovascular and cerebrovascular disease.
Nanometer liposome is the ultra micro spheroid carrier preparation that drug encapsulation is formed in lipoids bilayer.Liposome, as a kind of novel pharmaceutical carrier, has obtained development rapidly in preparation for external application to skin research and application aspect in recent years.Nanometer liposome as the feature of percutaneous drug delivery carrier is: compatibility, can strengthen the transdermal effect of medicine; Skin histology targeting; Slow-releasing; Liposomal encapsulated drug solubility and the stability of improving.The advantage such as that nanometer liposome external preparation has is efficient, long-acting, safety, skin targeting are strong.Exploitation external preparation for skin nano liposome preparations is to improve enepidermic trend, has become the direction of external used medicine and cosmetics research and development.
Have not yet to see the report of the application of Semen Ginkgo extrac nanometer liposome in anti-acne and treatment dermatosis.Therefore, about Semen Ginkgo extrac nanometer liposome external preparation is prevented and treated the research and development of dermatosis and seat skin ulcer newtype drug, cosmetics, disinfectant, and the abundant exploitation to gingko resource, will produce huge society and economic benefit.
Summary of the invention
The object of this invention is to provide the application of Semen Ginkgo extrac nanometer liposome in treatment dermatosis and anti-acne.Semen Ginkgo extrac nanometer liposome external preparation has the function of the infectious and allergic skin disease of control, have evident in efficacy, effect is extensive, use safe feature.
The object of the present invention is achieved like this:
The application of Semen Ginkgo extrac nanometer liposome external preparation in anti-acne and treatment dermatosis is provided.
Described Semen Ginkgo extrac is by Chemical Decomposition method, from the leaf of Semen Ginkgo, really, obtain the extract at the position such as episperm, rhizome, extracts solvent and can be the organic solvents such as ethanol, water, ether, petroleum ether; Or to Folium Ginkgo, really, episperm, rhizome extract elaboration products and in remaining waste material, again reclaim extracting in the course of processing.
Described Semen Ginkgo extrac nanometer liposome, by adopting the preparation of thin-film ultrasonic-high pressure homogenization method.
Described dermatosis is antibacterial, virus, fungus, parasite insect infection dermatoses; And dermatitis and anaphylaxis dermatosis.
Further, described dermatosis is seat skin ulcer, impetigo, folliculitis, herpes, wart, tinea, eczema, urticaria, skin pruritus, dermatitis, acariasis, scabies, tinea unguium, clavus callus, psoriasis multiple dermatosis.
The dermopathic medicine of the treatment that contains Semen Ginkgo extrac nanometer liposome, cosmetics or disinfectant external preparation are also provided, contain Semen Ginkgo extrac nanometer liposome 0.001-80%, and conventional external preparation substrate or external preparation acceptable carrier pharmaceutically.
Further, contain Semen Ginkgo extrac nanometer liposome 0.001-30%.
Further, contain Semen Ginkgo extrac nanometer liposome 0.5-20%.
By above-mentioned weight ratio scope get principal agent Semen Ginkgo extrac nanometer liposome, pharmaceutically conventional external preparation substrate or acceptable carrier prepares Semen Ginkgo extrac nanometer liposome external preparation; And comprise Semen Ginkgo extrac nanometer liposome and any combinations of substances is used for the treatment of dermopathic medicine, cosmetics or disinfectant external preparation.Described preparation is said exterior-applied formulation on any pharmaceutics, can be liquid preparation, cream cream, gel, membrane, lotion, powder, aerosol spray, suppository, transdermal absorption formulation etc.
The invention has the beneficial effects as follows, the present invention has significant therapeutic effect to seat skin ulcer, infectivity, struvite and anaphylaxis dermatosis tool, extensively, and curative effect is better than glucocorticoid or antibiotic in effect, without the side effect due to life-time service glucocorticoid, antibiotic.What is more important, the effect of Semen Ginkgo extrac nanometer liposome is significantly better than Semen Ginkgo extrac matched group.Local irritation test research shows safety of the present invention, without untoward reaction such as skin irritation, allergy.Clinical various skin patient's result on trial shows, the present invention has significant therapeutic effect to multiple dermatosis tool, and effective percentage reaches 100%, uses safety to have no side effect.
The specific embodiment
Below in conjunction with embodiment, the present invention is further described.
Embodiment 1:
By Semen Ginkgo peeling, put into tissue mashing machine and pulverize.Accurately take a certain amount of Semen Ginkgo through pulverizing, adding solid-liquid ratio is 1:10(W/V) 80% ethanol, at 60 ℃ of bath temperatures, stirring and leaching 2 times, each 4 hours extraction times.After lixiviate finishes, centrifugal (5000 revs/min, 15 minutes), get supernatant.Use vacuum rotary evaporator concentrated supernatant, condition is 55 ℃ of temperature, pressure 0.008-0.009MPa, and concentrating under reduced pressure, and in 60 ℃ of vacuum dryings, obtain Semen Ginkgo extrac extractum, and character is: light yellow extractum, be dissolved in alcohol, and water insoluble.
Phospholipid ethanol solution, filters; Add above-mentioned Semen Ginkgo extrac extractum, vitamin E, jolting is even, puts in round-bottomed flask, 30 ℃ of constant temperature water bath rotary evaporations, decompression film forming, makes film material on bottle wall, form uniform class membrane of lipoprotein, adds at 50 ℃ of PBS buffer fully vibration aquation, come off completely to wall upper film, immerse 50 ℃ of stirred in water bath, ultrasonic 5min under 25 ℃ of water bath condition, the each 5s of 200Hz, intermittently 5s, to forming the translucent turbid liquid that shows slightly opalescence.High pressure homogenize, circulates 3 times under 100 Mpa pressure, crosses microporous filter membrane, obtains light yellow transparent colloid shape Semen Ginkgo extrac nanometer liposome suspension.
The preparation of tincture: get above-mentioned Semen Ginkgo extrac nanometer liposome suspension 100ml, be dissolved in 600ml 95% alcoholic solution, add wherein again propylene glycol 200ml to stir, mend 95% alcoholic solution to enough 1000 ml, obtain faint yellow Semen Ginkgo extrac nanometer liposome tincture 1000 ml, Semen Ginkgo extrac nano-lipid body burden is 10%.Be sub-packed in 50 tubules every 20 ml.
Embodiment 2:
By Semen Ginkgo peeling, put into tissue mashing machine and pulverize.Accurately take a certain amount of Semen Ginkgo through pulverizing, adding solid-liquid ratio is 1:10(W/V) distilled water, at 60 ℃ of bath temperatures, stirring and leaching 2 times, each 4 hours extraction times.After lixiviate finishes, centrifugal (5000 revs/min, 15 minutes), get supernatant.Concentrated supernatant, and in 60 ℃ of vacuum dryings, obtain Semen Ginkgo extrac extractum, character is: light yellow extractum, be dissolved in alcohol, and water insoluble.
By embodiment 1 Semen Ginkgo extrac nanometer liposome suspension preparation technology, prepare Semen Ginkgo extrac nanometer liposome suspension.
The preparation of gel: carbopol 10g is sprinkled upon to 500ml distilled water surface, swelling spending the night.Take glycerol 60g, propylene glycol 120ml, is added to respectively in swelling good carbopol, grinds evenly rapidly, obtains transparent gel-type vehicle.Get above-mentioned Semen Ginkgo extrac nanometer liposome suspension 40ml, be dissolved in 100ml 95% alcoholic solution, be slowly added in gel-type vehicle, stir.Slowly add triethanolamine 15ml again, mend distilled water to enough 1000 g, obtain faint yellow Semen Ginkgo extrac nanometer liposome gel 1000 g, Semen Ginkgo extrac nano-lipid body burden is 4%.Be sub-packed in 50 tubules every 20 g.
Embodiment 3:
By Semen Ginkgo peeling, put into tissue mashing machine and pulverize.Accurately take a certain amount of Semen Ginkgo through pulverizing, adding solid-liquid ratio is 1:10(W/V) 80% petroleum ether, at 60 ℃ of bath temperatures, stirring and leaching 2 times, each 4 hours extraction times.After lixiviate finishes, centrifugal (5000 revs/min, 15 minutes), get supernatant.Use vacuum rotary evaporator concentrated supernatant, condition is 55 ℃ of temperature, pressure 0.008-0.009MPa, and concentrating under reduced pressure, and in 60 ℃ of vacuum dryings, obtain Semen Ginkgo extrac extractum, and character is: light yellow extractum, be dissolved in alcohol, and water insoluble.
By embodiment 1 Semen Ginkgo extrac nanometer liposome suspension preparation technology, prepare Semen Ginkgo extrac nanometer liposome suspension.
The preparation of ointment: get above-mentioned Semen Ginkgo extrac nanometer liposome suspension 5ml, be dissolved in 100ml 95% alcoholic solution, then add wherein triethanolamine 50ml, heat to 70 ℃.Paraffin 300g, stearic acid 300g, propylene glycol 100ml are added respectively to stir and melt, mend distilled water to enough 1000 g, stir to paste, obtain faint yellow Semen Ginkgo extrac nanometer liposome ointment 1000g, Semen Ginkgo extrac nano-lipid body burden is 0.5%.Be sub-packed in 50 tubules every 20 g.
Embodiment 4:
Folium Ginkgo is shredded, put into pulverizer by its pulverizing.Accurately take a certain amount of Folium Ginkgo through pulverizing, adding solid-liquid ratio is 1:6(W/V) 80% ethanol, at 60 ℃ of bath temperatures, stirring and leaching 2 times, each 4 hours extraction times.After lixiviate finishes, centrifugal (5000 revs/min, 15 minutes), get supernatant.Use vacuum rotary evaporator concentrated supernatant, condition is 55 ℃ of temperature, pressure 0.008-0.009MPa, and concentrating under reduced pressure, and in 60 ℃ of vacuum dryings, obtain Semen Ginkgo extrac extractum, and character is: light yellow extractum, be dissolved in alcohol, and water insoluble.
By embodiment 1 Semen Ginkgo extrac nanometer liposome suspension preparation technology, prepare Semen Ginkgo extrac nanometer liposome suspension.
Get above-mentioned Semen Ginkgo extrac nanometer liposome suspension 0.1ml, by embodiment 3 ointment preparation technologies, prepare Semen Ginkgo extrac nanometer liposome ointment 1000g, Semen Ginkgo extrac nano-lipid body burden is 0.01%.Be sub-packed in 50 tubules every 20 g.
Embodiment 5:
Folium Ginkgo is shredded, put into pulverizer by its pulverizing.Accurately take a certain amount of Folium Ginkgo through pulverizing, adding solid-liquid ratio is 1:6(W/V) distilled water, at 60 ℃ of bath temperatures, stirring and leaching 2 times, each 4 hours extraction times.After lixiviate finishes, centrifugal (5000 revs/min, 15 minutes), get supernatant.Concentrated supernatant, in 60 ℃ of vacuum dryings, obtains Semen Ginkgo extrac extractum, and character is: light yellow extractum, be dissolved in alcohol, and water insoluble.
By embodiment 1 Semen Ginkgo extrac nanometer liposome suspension preparation technology, prepare Semen Ginkgo extrac nanometer liposome suspension.
Get above-mentioned Semen Ginkgo extrac nanometer liposome suspension 300ml, by embodiment 1 tincture preparation technology, prepare Semen Ginkgo extrac nanometer liposome tincture 1000 ml, Semen Ginkgo extrac nano-lipid body burden is 30%.Be sub-packed in 50 tubules every 20 ml.
Embodiment 6:
Folium Ginkgo is shredded, put into pulverizer by its pulverizing.Accurately take a certain amount of Folium Ginkgo through pulverizing, adding solid-liquid ratio is 1:6(W/V) petroleum ether, at 60 ℃ of bath temperatures, stirring and leaching 2 times, each 4 hours extraction times.After lixiviate finishes, centrifugal (5000 revs/min, 15 minutes), get supernatant.Use vacuum rotary evaporator concentrated supernatant, condition is 55 ℃ of temperature, pressure 0.008-0.009MPa, and concentrating under reduced pressure, and in 60 ℃ of vacuum dryings, obtain Semen Ginkgo extrac extractum, and character is: light yellow extractum, be dissolved in alcohol, and water insoluble.
By embodiment 1 Semen Ginkgo extrac nanometer liposome suspension preparation technology, prepare Semen Ginkgo extrac nanometer liposome suspension.
Get above-mentioned Semen Ginkgo extrac nanometer liposome suspension 20ml, by embodiment 2 gel preparation technologies, prepare Semen Ginkgo extrac nanometer liposome gel 1000 g, Semen Ginkgo extrac nano-lipid body burden is 2%.Be sub-packed in 50 tubules every 20 g.
Embodiment 7:
Ginkgo nut episperm is stripped down, dry, pulverized with pulverizer.Accurately take a certain amount of gingko episperm through pulverizing, adding solid-liquid ratio is 1:6(W/V) 80% ethanol, at 60 ℃ of bath temperatures, stirring and leaching 2 times, each 4 hours extraction times.After lixiviate finishes, use nutsch filter sucking filtration, collect filtrate.Use vacuum rotary evaporator concentrated filtrate, condition is 55 ℃ of temperature, pressure 0.008-0.009MPa, concentrating under reduced pressure.In 60 ℃ of vacuum dryings, obtain Semen Ginkgo extrac extractum, character is: light brown extractum, micro-smelly, be dissolved in alcohol, water insoluble.
By embodiment 1 Semen Ginkgo extrac nanometer liposome suspension preparation technology, prepare Semen Ginkgo extrac nanometer liposome suspension.
Get above-mentioned Semen Ginkgo extrac nanometer liposome suspension 400ml, by embodiment 1 tincture preparation technology, prepare Semen Ginkgo extrac nanometer liposome tincture 1000ml, Semen Ginkgo extrac nano-lipid body burden is 40%.Be sub-packed in 50 tubules every 20 ml.
Embodiment 8:
Ginkgo nut episperm is stripped down, dry, pulverized with pulverizer.Accurately take a certain amount of gingko episperm through pulverizing, adding solid-liquid ratio is 1:6(W/V) 80% petroleum ether, at 60 ℃ of bath temperatures, stirring and leaching 2 times, each 4 hours extraction times.After lixiviate finishes, use nutsch filter sucking filtration, collect filtrate.Use vacuum rotary evaporator concentrated filtrate, condition is 55 ℃ of temperature, pressure 0.008-0.009MPa, concentrating under reduced pressure.In 60 ℃ of vacuum dryings, obtain Semen Ginkgo extrac extractum, character is: light brown extractum, micro-smelly, be dissolved in alcohol, water insoluble.
By embodiment 1 Semen Ginkgo extrac nanometer liposome suspension preparation technology, prepare Semen Ginkgo extrac nanometer liposome suspension.
Get above-mentioned Semen Ginkgo extrac nanometer liposome suspension 200ml, by embodiment 1 tincture preparation technology, prepare Semen Ginkgo extrac nanometer liposome tincture 1000 ml, Semen Ginkgo extrac nano-lipid body burden is 20%.Be sub-packed in 50 tubules every 20 ml.
Embodiment 9:
By embodiment 1 Semen Ginkgo extrac extractum preparation technology, prepare Semen Ginkgo extrac extractum.
By embodiment 1 Semen Ginkgo extrac nanometer liposome suspension preparation technology, prepare Semen Ginkgo extrac nanometer liposome suspension.
Get above-mentioned Semen Ginkgo extrac nanometer liposome suspension 1ml, by embodiment 3 ointment preparation technologies, prepare Semen Ginkgo extrac nanometer liposome ointment 1000g, Semen Ginkgo extrac nano-lipid body burden is 0.1%.Be sub-packed in 50 tubules every 20 g.
Embodiment 10:
By embodiment 4 Semen Ginkgo extrac extractum preparation technologies, prepare Semen Ginkgo extrac extractum.
By embodiment 1 Semen Ginkgo extrac nanometer liposome suspension preparation technology, prepare Semen Ginkgo extrac nanometer liposome suspension.
Get above-mentioned Semen Ginkgo extrac nanometer liposome suspension 10ml, by embodiment 2 gel preparation technologies, prepare Semen Ginkgo extrac nanometer liposome gel 1000g, Semen Ginkgo extrac nano-lipid body burden is 1%.Be sub-packed in 50 tubules every 20 g.
The Semen Ginkgo extrac nanometer liposome external preparation of making in order to top method, carries out relevant local irritation safety evaluatio experiment and clinic trial test.Pharmacology pharmacodynamic of the present invention and the experiment of local irritation safety evaluatio and data below.
Experiment one: to the therapeutical effect of chemical contact scytitis
Chemistry contact scytitis modelling: experiment is faced upward position with rabbit and fixed, abdominal part cropping is also used depilatory cream unhairing, at the border circular areas of the skin of abdomen surface markers 4 diameter 2.5cm of place, each region all covers with 3 layers of sterile gauze, dimethylbenzene is dripped on gauze, and it is moistening to continue to add dimethylbenzene maintenance gauze, observes scytitis reaction, continue to smear dimethylbenzene after 100 minutes, modeling success.
Get 10 of modeling success rabbit, there is II degree, the inflammatory reaction of III degree in local skin, shows as erythema, infiltration, pimple, vesicle etc.At 4 place's inflammation parts of same rabbit, smear respectively physiological saline solution, Semen Ginkgo extrac nanometer liposome gel of the present invention, Semen Ginkgo extrac gel, dexamethasone acetate ointment, every day 2 times, for three days on end.Observe the state of the each administered area of rabbit and control zone dermatitis.Dermatitis calibration and efficacy determination: the grade scale that dermatitis criterion is recommended according to international contact dermatitis seminar: "-" negative reaction, " ± " suspicious reaction (only having slight erythema), "+" weak positive reaction (erythema, infiltration, can have a small amount of pimple), " ++ " strong positive reaction (erythema, infiltration, pimple, vesicle), "+++" extremely strong reaction (erythema, infiltration, pimple, the large vesicle of appearance).Efficacy determination: inflammation transfers "-" to by " ++ " is above, is decided to be recovery from illness; Inflammation transfers " ± " to by " ++ " is above, is decided to be effective; Inflammation transfers "+" to by " ++ " is above, is decided to be effectively; Inflammation keeps " ++ " spend or deteriorate to " +++ " after medication, and it is invalid to be decided to be.
Experimental result shows, Semen Ginkgo extrac nanometer liposome gel of the present invention has significant therapeutic effect to chemical contact dermatitis, and effect is significantly better than Semen Ginkgo extrac matched group; And normal saline matched group to chemical contact dermatitis without therapeutical effect.The present invention, in application process, has the good effect of stripping off to the horny layer of disease damage skin part, can comparatively fast remove the disease damage tissue of lesions position, and affected area wound is healed rapidly.In application process of the present invention, have no apparent side effect, stop observation post administration 3 days, the phenomenon that has no dermatitis recurrence or increase the weight of, i.e. " rebound phenomenon "; And dexamethasone acetate group has 2 place's inflammation parts to occur that scytitis reaction increases the weight of.
The therapeutical effect of table 1. to chemical contact scytitis
| Grouping | Cure rate/% | Obvious effective rate/% | Onset time/min |
| Semen Ginkgo extrac | 60 | 80 | 14.1±2.6 |
| Semen Ginkgo extrac nanometer liposome | 90 | 100 | 9.1±2.0 ** |
| Dexamethasone acetate | 70 | 80 | 13.6±3.3 |
X ± s. n=10. compared with Semen Ginkgo extrac group, *
p<0.05, * *
p<0.01.
Experiment two: to the therapeutical effect of 2,4 dinitrochlorobenzene (DNCB) induced mice type Ⅳ allergy
40 of mices, are divided into 4 groups at random: model contrast, Semen Ginkgo extrac nanometer liposome tincture of the present invention, Semen Ginkgo extrac tincture, hydrocortisone emulsifiable paste group, 10 every group.Test 1d, respectively organize every mouse subcutaneous injection 3.5% DNCB-acetone oil solution 0.04ml sensitization.Test 10d, in each mouse right ear tow sides, evenly coat 1% DNCB-acetone oil solution 0.03ml and attack.Test 11d, each mouse right ear of model control group be outer 75% alcoholic solution that is coated with respectively, and each medication group mouse right ear is the outer relative medicine that is coated with respectively, every day 2 times, for three days on end.Last is smeared and is put to death mice in latter 24 hours, takes off left and right auricle with diameter 6mm card punch, weighs, using the difference of two auricle weight as type Ⅳ allergy intensity on analytical balance.Treatment rear right auricular concha skin lesion pathological change and infiltrating cells are observed: under HE stained, observe auricular concha pathological change, under light microscopic, with ocular-net micrometer, count every square millimeter of number of inflammatory cells (comprising Streptococcus and polymorphonuclear leukocyte).Experimental result is in Table 2.Result shows, Semen Ginkgo extrac nanometer liposome external preparation of the present invention has obvious inhibitory action to the ear swelling of dermatitis model mouse, and can obviously reduce skin lesion position inflammatory cell infiltration, has antiinflammatory action.Illustrate that the present invention can alleviate the inflammatory reactions such as hyperemia, edema, effect is better than glucocorticoid hydrocortisone and Semen Ginkgo extrac.In application process of the present invention, have no apparent side effect.
Table 2. causes the impact of mice type Ⅳ allergy on DNCB
| Grouping | Swelling/mg | Suppression ratio/% | Inflammatory cell counting //mm 2 |
| Model contrast | 16.47±4.07 | － | 684.01±112.39 |
| Semen Ginkgo extrac | 10.22±2.83 ** | 37.95 | 435.23±81.46 ** |
| Semen Ginkgo extrac nanometer liposome | 7.04±1.95 **# | 57.26 | 269.02±64.30 **## |
| Hydrocortisone | 9.34±2.72 ** | 43.29 | 314.58±75.04 **# |
X ± s. n=10. compared with model group,
* p<0.05,
* p<0.01. compared with Semen Ginkgo extrac group,
# p<0.05,
## p<0.01.
Experiment three: to the effect of bacterial infection treating for skin disease
Microbionation is cultivated: the staphylococcus aureus of clinical separation and seat skin ulcer propionibacterium are identified, be inoculated in culture medium 35 ℃ of continuous culture.During experimental infection, be diluted to 1X10 with fluid medium
7individual/m l is standby.The preparation of skin infection model: the rabbit of selecting the rabbit ear to infect without skin lesion, in rabbit ear inner side, prepare the skin lesion that diameter is 7mm, until hemorrhage, with aseptic cotton balls hemostasis by compression.After stopping blooding, with the 1m l syringe of being furnished with No. 4 syringe needles, carefully at surperficial 2 staphylococcus aureuses or the seat skin ulcer propionibacterium liquid of dripping of skin lesion, rabbit ear skin lesion place is set level, treat its natural drying, make skin infection model.
40 of rabbit, 80 of the rabbit ears, divide right and left, and serial number is divided into 8 groups, every group of 10 rabbit ears.First 4 groups are infected staphylococcus aureus for first 24 hours in administration, and latter 4 groups are infected seat skin ulcer propionibacterium.Medicine for external application or physiological saline solution after 24 hours, every day 2 times, for three days on end, observe and record the variation of the red and swollen area of skin infection.The results are shown in Table 3.Experimental result shows, staphylococcus aureus and seat skin ulcer propionibacterium skin infection be after 24 hours, local skin redness, and temperature raises, after outer painting Semen Ginkgo extrac nanometer liposome of the present invention ointment, skin infection is had to remarkable inhibitory action, red and swollen area obviously dwindles; Effect is better than ofloxacin ointment and Semen Ginkgo extrac.In application process of the present invention, have no apparent side effect.
The therapeutical effect that table 3. infects skin staphylococcus aureus, seat skin ulcer propionibacterium
X ± s. n=10. compared with model group, *
p<0.05, * *
p<0.01. compared with Semen Ginkgo extrac group,
# p<0.05,
## p<0.01.
Experiment four: to the therapeutical effect of herpesvirus infection dermatoses
Get 40 of Cavia porcelluss, be divided at random 4 groups: model contrast, Semen Ginkgo extrac nanometer liposome of the present invention, Semen Ginkgo extrac, Virless Cream group, 10 every group.The foundation of infection model: by guinea pig back unhairing, area 4cmX4cm, penetrates into deep skin with syringe needle, is evenly thorn 7 pins of Flos Mume sample in 1-1.2cm diameter, with former times of herpes simplex virus of clinical separation (HSV-1) liquid, 0.07ml drips in acupuncture district, with aseptic glass rod, is coated with and opens infection.In infecting the 5th day, each administration group is coated with relative medicine outward, and model control group is coated with physiological saline solution outward.Treat every day for 2 times, continuous 6 days.Observed and recorded every day infected area skin lesion degree, is divided into 4 grades: 0 grade: skin is normal or recover incrustation and come off; 1 grade: infection face is rubescent or recovery incrustation slightly; 2 grades: infect flushing swollen; 3 grades: infect flushing swollen heavier or with herpes.Statistical procedures: each group skin lesion degree of each term day is compared with rank test (two groups of sequence checkings) method; With following formula, calculate each term day skin lesion degree: average disease damage degree=Σ (certain level infects face number × this grade of index)/this group infects face sum.The results are shown in Table 4.Experimental result shows; Semen Ginkgo extrac nanometer liposome gel of the present invention can obviously suppress herpesvirus (HSV-1) to be infected; the guinea pig skin of herpesvirus infection is had to significant protective effect; remarkable with model control group comparing difference, and effect is better than acyclovir and Semen Ginkgo extrac.In application process of the present invention, the horny layer of disease damage skin part is had to the good effect of stripping off, can comparatively fast remove the disease damage tissue of lesions position, affected area wound is healed rapidly.In application process of the present invention, have no apparent side effect.
The therapeutical effect of table 4. to herpesvirus infection guinea pig skin disease
X ± s. n=10. compared with model group, *
p<0.05, * *
p<0.01. compared with Semen Ginkgo extrac group,
# p<0.05,
## p<0.01.
Experiment five: to the therapeutical effect of cutaneous fungal infection
Trichophyton gypseum suspension preparation: trichophyton gypseum activates after 10 days in 10 ml test tubes (including potato dextrose agar 3 ml) at 30 ℃, to in vitro adding 1ml normal saline (containing 0.1% Tween 80), suction pipe aspirates gently, get containing the bacteria suspension of mycelia piece and put into dismembyator grinding evenly, naked eyes are without obvious granule, be ground-glass appearance outward appearance, Microscopic observation mycelia, with Sharpe fluid medium adjustment bacteria suspension concentration to 2 X 10
5cfu/ml is standby.Sporidiole tinea collecting cells method is the same.The foundation of infected animal model: in guinea pig back both sides apart from atlas 5cm and 15cm and be the center of circle apart from 4 intersection points of back center line 5cm, with the circle depilation face of hand-lifting diameter 10cm processed, after 24 hours, with 1ml syringe, at 30 °, the face center of circle of depilation angle head skew back, thrust (not make dermatorrhagia as degree) in guinea pig skin, inject bacteria suspension 0.02ml, separately get a fritter lawn and implant in pin hole, it is embedded on guinea pig skin.Infect animal skin after 3-5 days and occur the anthropoid tinea corporis infection symptoms of class, model success is described.Get trichophyton gypseum and infect 40 of guinea pig models, be divided into 4 groups, i.e. Semen Ginkgo extrac nanometer liposome of the present invention, Semen Ginkgo extrac, Miconazole Nitrate Cream, model control group, 10 every group.Each administration group is coated with relative medicine outward, and model control group is coated with physiological saline solution outward, and 2 times/day, Continuous Observation medication 10 days.Sporidiole trichophyton mentagrophytes infection guinea pig model grouping administration is infected guinea pig model with trichophyton gypseum.Observation index: infect sign in the 0th, 3,7,10 days gross examination of skeletal muscle skin lesion squamas for the treatment of, erythema, abscess etc., skin lesion position row fungus microscope examination and cultivation, measure skin lesion diameter, the reflection skin lesion area take diameter as index.Treatment standard: skin lesion place is without any infection sign, and fungus microscope examination, cultivation are all negative for curing; Before a small amount of pimple of inoculation position and erythema, paler colour or treatment, take an evident turn for the better as effectively; Erythema diffusion, accompanies desquamation, pimple, local abscess or even more serious diffusion, injures the thick crust of corium, and even hemorrhage is invalid.The results are shown in Table 5-7.Experimental result shows, Semen Ginkgo extrac nanometer liposome ointment of the present invention has significant therapeutic effect to trichophyton gypseum and sporidiole trichophyton mentagrophytes infection guinea pig skin tool, can make to infect skin lesion diameter and obviously be contracted to disappearance, skin lesion squama, erythema, abscess etc. infect sign obviously to be alleviated to disappearing, 10 days fungus microscope examinations, cultivate all negative; Curative effect is better than Miconazole Nitrate Cream and Semen Ginkgo extrac.In application process of the present invention, the horny layer of disease damage skin part is had to the good effect of stripping off, can comparatively fast remove the disease damage tissue of lesions position, affected area wound is healed rapidly.In Semen Ginkgo extrac nanometer liposome salve application process of the present invention, have no apparent side effect.
The therapeutic effect of table 5. to trichophyton gypseum and sporidiole trichophyton mentagrophytes infection
Table 6. trichophyton gypseum infects skin lesion diameter (mm) to be changed
| Grouping | 0 day | 3 days | 7 days | 10 days |
| Model contrast | 8.14±1.91 | 8.82±2.45 | 9.39±2.41 | 9.92±2.31 |
| Semen Ginkgo extrac | 7.95±2.06 | 6.13±1.82 * | 4.85±0.92 ** | 2.44±0.25 ** |
| Semen Ginkgo extrac nanometer liposome | 8.03±2.33 | 5.47±1.18 ** | 2.46±0.77 **## | 0.17±0.05 **## |
| Miconazole nitrate | 7.96±2.09 | 6.02±1.76 ** | 4.03±1.05 ** | 1.46±0.22 **## |
X ± s. n=10. compared with model group, *
p<0.05, * *
p<0.01. compared with Semen Ginkgo extrac group,
# p<0.05,
## p<0.01.
Table 7. sporidiole trichophyton mentagrophytes infection skin lesion diameter (mm) changes
| Grouping | 0 day | 3 days | 7 days | 10 days |
| Model contrast | 6.01±1.45 | 6.79±1.99 | 7.80±1.97 | 8.59±2.23 |
| Semen Ginkgo extrac | 5.92±1.73 | 4.26±1.07 ** | 2.32±0.94 ** | 1.42±0.27 ** |
| Semen Ginkgo extrac nanometer liposome | 5.99±1.64 | 3.55±0.85 ** | 1.51±0.54 **# | 0.28±0.09 **## |
| Miconazole nitrate | 5.87±1.51 | 4.83±1.42 * | 2.17±0.51 ** | 1.24±0.26 ** |
X ± s. n=10. compared with model group, *
p<0.05, * *
p<0.01. compared with Semen Ginkgo extrac group,
# p<0.05,
## p<0.01.
Experiment six: treating for skin disease effect due to inhibition acarid
Demodex Parasitized is one of reason of folliculitis and the morbidity of sebaceous gland chronic inflammatory disease dermatosis.Experiment is for examination acarid: human body face vermiform mite, the person's face of taking from mite infection.Before sleeping evening, person under inspection cleans face, the adhesive tape of 1.5cm × 6cm is affixed on to nose and a side nasolabial fold portion, take off adhesive tape and be affixed on microscopy on microscope slide inferior morning, on every adhesive tape, having more than 3 person of adult is experimenter, select altogether 30 parts, be divided at random 3 groups, be i.e. Semen Ginkgo extrac nanometer liposome of the present invention, Semen Ginkgo extrac, matched group, every group 10 parts, count out every group of acarid sum.Semen Ginkgo extrac nanometer liposome tincture group of the present invention: the adhesive tape glue that meets experiment condition is faced up and is fixed on microscope slide, evenly drip Semen Ginkgo extrac nanometer liposome tincture of the present invention on microscope slide, thin and thick observing polypide as standard under high power lens, and for keeping contacting of medicine and polypide on slide, supplement at any time medicinal liquid, every slide is fixed on a micro-Microscopic observation, within every 2 hours, observe 1 time, each observation 30 seconds, record the active situation of polypide, until polypide is motionless under high power lens, confirm polypide death.Semen Ginkgo extrac tincture group: choosing meets 10 parts of the adhesive tapes of tested condition, drips Semen Ginkgo extrac tincture, observe and decision method the same.Matched group: choosing meets 10 parts of the adhesive tapes of tested condition, drips 75% alcoholic solution, observe and decision method the same.The results are shown in Table 8.Experimental result shows, Semen Ginkgo extrac nanometer liposome tincture of the present invention has the effect of killing acarid, can suppress the dermatosis due to mite infection, the Semen Ginkgo extrac that is better than evident in efficacy.
Table 8. acaricidal action experimental result (the borer population mortality rate of living)
Local irritation test: get 8 of adult rabbit, male and female half and half, every animal sub-cage rearing, first raises 3-4 days at laboratory condition.Then rabbit spinal column both sides dorsal body setae is shaved off to every lateral area 5 × 10cm with electric shaver-for women
2.Carefully check that whether depilation district has redness and damage, if any abandoning it, can not test.Adopt the test method of multiple dosing, in depilation, Semen Ginkgo extrac nanometer liposome external preparation of the present invention is smeared in district, every day 2 times, smears continuously 7 days.Whether last is smeared after 24 hours and is washed away smear with warm water, observes local skin reaction respectively at l, after 24,48,72 hours, as erythema, edema, desquamation, incrustation etc., have petechia, skin peptide coarse or poor etc., records time of origin and regression time.Experimental result shows, is smearing continuously Semen Ginkgo extrac nanometer liposome external preparation of the present invention after 7 days, and each rabbit skin does not all show obvious skin irritation and anaphylaxis.Show product safety of the present invention, have no adverse reaction.
[0059] clinic trial test: the Semen Ginkgo extrac nanometer liposome external preparation of making in order to top method, on probation through various skin patient, concrete case and the course for the treatment of are in Table 9.All patients are coated with sufferer skin lesion place outward with the present invention, every day 2 times.Patient does not all use any other external preparation for skin medicine during treating.Criterion of therapeutical effect: cure: clinical skin lesion performance all disappears, and pathogen microscopy is negative; Effective: the basic disappearance of clinical skin lesion performance, pathogen microscopy is the weak positive; Effective: clinical skin lesion performance part disappears, and pathogen microscopy is the weak positive; Invalid: clinical skin lesion shows constant or increases the weight of, and pathogen microscopy is positive.Cure rate=healing number/total case X100%; Effective percentage=(healing number+effective number+efficiently individual quantity)/total case X100%.The results are shown in Table 9.Clinic trial result shows, acne and various skin patient use Semen Ginkgo extrac nanometer liposome external preparation of the present invention all effective, and effective percentage reaches 100%; Cure rate all exceedes 80%.During application the present invention, all there is not the untoward reaction such as skin irritation, allergy in all patients.Therefore the present invention is the dermopathic external preparation of a kind of desirable treatment, evident in efficacy, safe and reliable.
Table 9 Semen Ginkgo extrac nanometer liposome of the present invention external preparation is treated dermopathic clinical effectiveness
The present invention is compared with prior art: the present invention derives from natural precious medicinal plants Semen Ginkgo, from Folium Ginkgo, really, the position such as episperm extracts and obtains Semen Ginkgo extrac, take Semen Ginkgo extrac as effective ingredient, make nanometer liposome preparation for external application to skin, infectious, anaphylaxis dermatosis tool are had significant therapeutic effect, and curative effect is better than glucocorticoid or antibiotic, without the side effect due to life-time service glucocorticoid, antibiotic.Therefore Semen Ginkgo extrac nanometer liposome external preparation of the present invention is evident in efficacy, uses safety, has no adverse reaction, and has good potential applicability in clinical practice.
Claims (7)
1. Semen Ginkgo extrac nanometer liposome is prevented and treated the purposes in the external preparation of dermatosis and anti-acne in preparation.
2. purposes as claimed in claim 1, described dermatosis is antibacterial, virus, fungus, parasite insect infection dermatoses; And struvite and anaphylaxis dermatosis.
3. Semen Ginkgo extrac nanometer liposome as claimed in claim 1, described Semen Ginkgo extrac is by Chemical Decomposition method, from the leaf of Semen Ginkgo, really, obtain the extract at episperm, rhizome position, extraction solvent is ethanol, water, ether, petroleum ether organic solvent; Or to Folium Ginkgo, really, episperm, rhizome extract elaboration products and in remaining waste material, again reclaim extracting in the course of processing; Described Semen Ginkgo extrac nanometer liposome, by adopting the preparation of thin-film ultrasonic-high pressure homogenization method.
4. external preparation as claimed in claim 1 is the dermopathic medicine for the treatment of, cosmetics or the disinfectant external preparation that contain Semen Ginkgo extrac nanometer liposome; And comprise Semen Ginkgo extrac nanometer liposome and any combinations of substances is used for the treatment of dermopathic medicine, cosmetics or disinfectant external preparation; It is characterized in that, described preparation is said exterior-applied formulation on any pharmaceutics, is liquid preparation, cream cream, gel, powder, membrane, lotion, aerosol spray, transdermal absorption formulation.
5. medicine as claimed in claim 4, cosmetics or disinfectant external preparation, is characterized in that, contains Semen Ginkgo extrac nanometer liposome 0.001-80%.
6. medicine as claimed in claim 4, cosmetics or disinfectant external preparation, is characterized in that, contains Semen Ginkgo extrac nanometer liposome 0.001-30%.
7. medicine as claimed in claim 4, cosmetics or disinfectant external preparation, is characterized in that, contains Semen Ginkgo extrac nanometer liposome 0.5-20%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310409572.0A CN103751231A (en) | 2013-09-11 | 2013-09-11 | Application of gingko extract nanometer liposome in acne removing and dermatosis treatment |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310409572.0A CN103751231A (en) | 2013-09-11 | 2013-09-11 | Application of gingko extract nanometer liposome in acne removing and dermatosis treatment |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN103751231A true CN103751231A (en) | 2014-04-30 |
Family
ID=50518658
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310409572.0A Pending CN103751231A (en) | 2013-09-11 | 2013-09-11 | Application of gingko extract nanometer liposome in acne removing and dermatosis treatment |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103751231A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107837215A (en) * | 2017-11-02 | 2018-03-27 | 河北荃美生物科技有限公司 | Bilobanoate beautifying and skin-protecting cream, preparation method and applications |
| CN114831908A (en) * | 2022-04-28 | 2022-08-02 | 江苏农牧科技职业学院 | Ginkgo episperm anti-aging acne-removing cream and preparation method thereof |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102247411A (en) * | 2011-08-18 | 2011-11-23 | 王青 | Application of ginkgo extractive in preparing external preparation capable of treating skin disease |
-
2013
- 2013-09-11 CN CN201310409572.0A patent/CN103751231A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102247411A (en) * | 2011-08-18 | 2011-11-23 | 王青 | Application of ginkgo extractive in preparing external preparation capable of treating skin disease |
Non-Patent Citations (2)
| Title |
|---|
| 江彬彬 等: "脂质体透皮吸收的研究进展", 《中国药房》 * |
| 江彬彬 等: "脂质体透皮吸收的研究进展", 《中国药房》, no. 06, 31 December 2002 (2002-12-31) * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107837215A (en) * | 2017-11-02 | 2018-03-27 | 河北荃美生物科技有限公司 | Bilobanoate beautifying and skin-protecting cream, preparation method and applications |
| CN114831908A (en) * | 2022-04-28 | 2022-08-02 | 江苏农牧科技职业学院 | Ginkgo episperm anti-aging acne-removing cream and preparation method thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN100569273C (en) | A kind of external Chinese medicine that is used for the treatment of skin tumour, tinea, wart, herpes | |
| CN101862351B (en) | Application of active parts of gallnut in preparing anti-ulcerative colitis medicine | |
| CN104688786A (en) | Externally used pharmaceutical composition, preparation method and application thereof | |
| CN102920915A (en) | Ointment preparation for treating haemorrhoids and preparation method for ointment preparation | |
| CN102319272B (en) | Application of ginkgolic acid in preparation of external preparation for treating dermatopathy | |
| CN105395593A (en) | Itching relieving ointment containing folium artemisiae argyi extract and preparation method thereof | |
| CN110974861B (en) | Blumea balsamifera oil liposome | |
| CN103751231A (en) | Application of gingko extract nanometer liposome in acne removing and dermatosis treatment | |
| CN101040957B (en) | Medicine for treating skin diseases such as eczema and dermatitis and the method for preparing the same | |
| CN110279717A (en) | The preparation of crocodile first active principle and its anti-oxidant, anti-hepatic fibrosis application | |
| CN102846677A (en) | Use of ginkgolic acids in preparation of external preparation for treating cutaneous tumors | |
| CN102349935A (en) | Purpose of ginkgolic acid in preparing external preparation for treating venereal disease, gynopathy and perianal disease | |
| CN105147827B (en) | A kind of antibacterial medicines and preparation method thereof | |
| CN103751230A (en) | Application of gingko extract nanometer liposome in gynecological disease treatment | |
| CN114224952A (en) | Traditional Chinese medicine decontamination preparation for soothing skin of infants and preparation method thereof | |
| CN103751105A (en) | Application of ginkgolic acid nanometer liposome in treatment of gynecological diseases and venereal diseases | |
| CN102145072A (en) | Traditional Chinese medicine composition for treating infantile eczema and dermatitis skin diseases | |
| CN102973680B (en) | External linimentum for treating superficial fungal infection and preparation method of external linimentum | |
| CN112294929A (en) | Gynecological traditional Chinese medicine salt lotion and preparation method and application thereof | |
| CN102302524A (en) | Applications of gingko extractive in preparing external preparation for treating venereal disease, gynecopathy and perianal diseases | |
| CN115300576B (en) | Compound traditional Chinese medicine nanogel for treating beriberi and tinea pedis and preparation method thereof | |
| CN102846678A (en) | Application of ginkgolic acid in weight losing | |
| CN102579629B (en) | Chinese medicinal preparation for treating vaginitis and preparation method thereof | |
| CN102526266B (en) | Chinese medicinal preparation for treating vaginitis and preparation method thereof | |
| CN111388535B (en) | Application of osmanthus flower extract in preparation of medicine for preventing and treating sunburn |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20140430 |