CN103751230A - Application of gingko extract nanometer liposome in gynecological disease treatment - Google Patents
Application of gingko extract nanometer liposome in gynecological disease treatment Download PDFInfo
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Abstract
The invention relates to a use of gingko extract nanometer liposome in preparation of external application preparations for gynecological disease treatment. According to the present invention, the gingko extract can be obtained from extracts of leaf, fruit, rhizome and other parts of gingko through a chemical separation method; the gingko extract nanometer liposome is prepared by using a film ultrasound-high pressure homogenization method; the drug, the cosmetic or the disinfectant external application preparation containing the gingko extract nanometer liposome and provided for gynecological disease treatment contains 0.001-80% of the gingko extract nanometer liposome and a pharmaceutically and commonly used external application preparation substrate or an acceptable carrier; the preparation is the any one external application formulation in the pharmacy; and results of pharmacology studies and clinical trials show that the gingko extract nanometer liposome provides significant treatment effects for a variety of gynecological diseases, and has characteristics of safe use and no side effect.
Description
Technical field
The invention belongs to biological medicine technology field, be specifically related to the purposes of Semen Ginkgo extrac nanometer liposome in the gynopathic external preparation of preparation treatment.
Background technology
Gynaecopathia is as trichomonal vaginitis, colpitis mycotica, bacterial vaginitis, pudendal pruritus, cervicitis, cervical erosion etc., be one of clinical modal disease, this type of disease persistent period is long, easily outbreak repeatedly, to patient, bring misery, have a strong impact on patient's physical and mental health.This type of disease for the treatment of mostly is antimicrobial drug at present, though these medicines have effect for the moment, after drug withdrawal, easily recurrence, can not reach the effect of wound repairing; And prolonged application can cause many side effect.For fear of the generation of this type of bad phenomenon, market demand develop a kind of derive from natural, effect significantly, without the gynopathic external preparation for the treatment of of obvious adverse reaction, to alleviate patient suffering.This type of Disease is numerous, and therefore the external preparation product of this type of disease of exploitation treatment has good potential applicability in clinical practice, to patient, is very useful and necessary.
Semen Ginkgo is the medicinal plants of China's preciousness, and leaf, fruit and the episperm etc. of Semen Ginkgo all have medicinal exploitation and are worth.According to < < Compendium of Materia Medica > >, record, Semen Ginkgo property is sweet, bitter, flat, puckery; Have that relieving asthma and cough, leukorrhagia stopping are turbid, the merit of pain relieving; Cure mainly cough of cold type phlegm dyspnea, asthma, frequent micturition, leucorrhea with red and white discharge, rhagadia manus et pedis etc.Particularly it is found that in recent decades in Folium Ginkgo and contain plurality of active ingredients, main effective ingredient is flavonoid and terpene lactone compound, has the function of control cardiovascular and cerebrovascular disease.
Nanometer liposome is the ultra micro spheroid carrier preparation that drug encapsulation is formed in lipoids bilayer.Liposome, as a kind of novel pharmaceutical carrier, has obtained development rapidly in preparation for external application to skin research and application aspect in recent years.Nanometer liposome as the feature of percutaneous drug delivery carrier is: compatibility, can strengthen the transdermal effect of medicine; Skin histology targeting; Slow-releasing; Liposomal encapsulated drug solubility and the stability of improving.The advantage such as that nanometer liposome external preparation has is efficient, long-acting, safety, skin targeting are strong.Exploitation external preparation for skin nano liposome preparations is to improve enepidermic trend, has become the direction of external used medicine and cosmetics research and development.
Have not yet to see the report of the application of Semen Ginkgo extrac nanometer liposome in treatment gynaecopathia.Therefore, about Semen Ginkgo extrac nanometer liposome external preparation is prevented and treated the research and development of gynaecopathia newtype drug, cosmetics, disinfectant, and the abundant exploitation to gingko resource, will produce huge society and economic benefit.
Summary of the invention
The object of this invention is to provide the new purposes of Semen Ginkgo extrac nanometer liposome, i.e. the purposes of Semen Ginkgo extrac nanometer liposome in the gynopathic external preparation of preparation control.It has evident in efficacy, use safe feature.
The object of the present invention is achieved like this:
The purposes of Semen Ginkgo extrac nanometer liposome in the gynopathic external preparation of preparation treatment is provided.
Described gynaecopathia is trichomonal vaginitis, colpitis mycotica, bacterial vaginitis, pudendal pruritus, cervicitis, cervical erosion etc.
Described Semen Ginkgo extrac is by Chemical Decomposition method, from the leaf of Semen Ginkgo, really, obtain the extract at the position such as rhizome, extracts solvent and can be the organic solvents such as ethanol, water, ether, petroleum ether; Or to Folium Ginkgo, really, rhizome extracts elaboration products and in remaining waste material, again reclaims extracting in the course of processing.
Described Semen Ginkgo extrac nanometer liposome, by adopting the preparation of thin-film ultrasonic-high pressure homogenization method.
The gynopathic medicine of the treatment that contains Semen Ginkgo extrac nanometer liposome, disinfectant external preparation are also provided, contain Semen Ginkgo extrac nanometer liposome 0.001-80%, and conventional external preparation substrate or external preparation acceptable carrier pharmaceutically.
Further, contain Semen Ginkgo extrac nanometer liposome 0.001-30%.
Further, contain Semen Ginkgo extrac nanometer liposome 0.5-20%.
By above-mentioned weight ratio scope get principal agent Semen Ginkgo extrac nanometer liposome, pharmaceutically conventional external preparation substrate or acceptable carrier prepares Semen Ginkgo extrac nanometer liposome external preparation; And comprise Semen Ginkgo extrac nanometer liposome and any combinations of substances is used for the treatment of gynopathic medicine, disinfectant external preparation.Described preparation is said exterior-applied formulation on any pharmaceutics, is liquid preparation, cream cream, gel, membrane, lotion, powder, aerosol spray, suppository, transdermal absorption formulation.
The invention has the beneficial effects as follows, pharmacology test research shows that the present invention has significant therapeutic effect to rat bacterial vaginitis, colpitis mycotica, cervicitis model tool, and can suppress the propagation of human papillomavirus (HPV) infection cell, there is the effect of anti-HPV viral infection; What is more important, the effect of Semen Ginkgo extrac nanometer liposome is significantly better than Semen Ginkgo extrac matched group.Local irritation test research shows safety of the present invention, without untoward reaction such as skin irritation, allergy.Clinical multiple gynecological patient result on trial shows, the present invention has significant therapeutic effect to the sick tool of multiple gynecological, and effective percentage reaches 100%, uses safety to have no side effect.
The specific embodiment
Below in conjunction with embodiment, the present invention is further described.
Embodiment 1:
By Semen Ginkgo peeling, put into tissue mashing machine and pulverize.Accurately take a certain amount of Semen Ginkgo through pulverizing, adding solid-liquid ratio is 1:10(W/V) 80% ethanol, at 60 ℃ of bath temperatures, stirring and leaching 2 times, each 4 hours extraction times.After lixiviate finishes, centrifugal (5000 revs/min, 15 minutes), get supernatant.Use vacuum rotary evaporator concentrated supernatant, condition is 55 ℃ of temperature, pressure 0.008-0.009MPa, and concentrating under reduced pressure, and in 60 ℃ of vacuum dryings, obtain Semen Ginkgo extrac extractum, and character is: light yellow extractum, be dissolved in alcohol, and water insoluble.
Phospholipid ethanol solution, filters; Add above-mentioned Semen Ginkgo extrac extractum, vitamin E, jolting is even, puts in round-bottomed flask, 30 ℃ of constant temperature water bath rotary evaporations, decompression film forming, makes film material on bottle wall, form uniform class membrane of lipoprotein, adds at 50 ℃ of PBS buffer fully vibration aquation, come off completely to wall upper film, immerse 50 ℃ of stirred in water bath, ultrasonic 5min under 25 ℃ of water bath condition, the each 5s of 200Hz, intermittently 5s, to forming the translucent turbid liquid that shows slightly opalescence.High pressure homogenize, circulates 3 times under 100 Mpa pressure, crosses microporous filter membrane, obtains light yellow transparent colloid shape Semen Ginkgo extrac nanometer liposome suspension.
The preparation of tincture: get above-mentioned Semen Ginkgo extrac nanometer liposome suspension 100ml, be dissolved in 600ml 95% alcoholic solution, add wherein again propylene glycol 200ml to stir, mend 95% alcoholic solution to enough 1000 ml, obtain faint yellow Semen Ginkgo extrac nanometer liposome tincture 1000 ml, Semen Ginkgo extrac nano-lipid body burden is 10%.Be sub-packed in 50 tubules every 20 ml.
Embodiment 2:
By Semen Ginkgo peeling, put into tissue mashing machine and pulverize.Accurately take a certain amount of Semen Ginkgo through pulverizing, adding solid-liquid ratio is 1:10(W/V) distilled water, at 60 ℃ of bath temperatures, stirring and leaching 2 times, each 4 hours extraction times.After lixiviate finishes, centrifugal (5000 revs/min, 15 minutes), get supernatant.Concentrated supernatant, and in 60 ℃ of vacuum dryings, obtain Semen Ginkgo extrac extractum, character is: light yellow extractum, be dissolved in alcohol, and water insoluble.
By embodiment 1 Semen Ginkgo extrac nanometer liposome suspension preparation technology, prepare Semen Ginkgo extrac nanometer liposome suspension.
The preparation of gel: carbopol 10g is sprinkled upon to 500ml distilled water surface, swelling spending the night.Take glycerol 60g, propylene glycol 120ml, is added to respectively in swelling good carbopol, grinds evenly rapidly, obtains transparent gel-type vehicle.Get above-mentioned Semen Ginkgo extrac nanometer liposome suspension 40ml, be dissolved in 100ml 95% alcoholic solution, be slowly added in gel-type vehicle, stir.Slowly add triethanolamine 15ml again, mend distilled water to enough 1000 g, obtain faint yellow Semen Ginkgo extrac nanometer liposome gel 1000 g, Semen Ginkgo extrac nano-lipid body burden is 4%.Be sub-packed in 50 tubules every 20 g.
Embodiment 3:
By Semen Ginkgo peeling, put into tissue mashing machine and pulverize.Accurately take a certain amount of Semen Ginkgo through pulverizing, adding solid-liquid ratio is 1:10(W/V) 80% petroleum ether, at 60 ℃ of bath temperatures, stirring and leaching 2 times, each 4 hours extraction times.After lixiviate finishes, centrifugal (5000 revs/min, 15 minutes), get supernatant.Use vacuum rotary evaporator concentrated supernatant, condition is 55 ℃ of temperature, pressure 0.008-0.009MPa, and concentrating under reduced pressure, and in 60 ℃ of vacuum dryings, obtain Semen Ginkgo extrac extractum, and character is: light yellow extractum, be dissolved in alcohol, and water insoluble.
By embodiment 1 Semen Ginkgo extrac nanometer liposome suspension preparation technology, prepare Semen Ginkgo extrac nanometer liposome suspension.
The preparation of ointment: get above-mentioned Semen Ginkgo extrac nanometer liposome suspension 5ml, be dissolved in 100ml 95% alcoholic solution, then add wherein triethanolamine 50ml, heat to 70 ℃.Paraffin 300g, stearic acid 300g, propylene glycol 100ml are added respectively to stir and melt, mend distilled water to enough 1000 g, stir to paste, obtain faint yellow Semen Ginkgo extrac nanometer liposome ointment 1000g, Semen Ginkgo extrac nano-lipid body burden is 0.5%.Be sub-packed in 50 tubules every 20 g.
Embodiment 4:
Folium Ginkgo is shredded, put into pulverizer by its pulverizing.Accurately take a certain amount of Folium Ginkgo through pulverizing, adding solid-liquid ratio is 1:6(W/V) 80% ethanol, at 60 ℃ of bath temperatures, stirring and leaching 2 times, each 4 hours extraction times.After lixiviate finishes, centrifugal (5000 revs/min, 15 minutes), get supernatant.Use vacuum rotary evaporator concentrated supernatant, condition is 55 ℃ of temperature, pressure 0.008-0.009MPa, and concentrating under reduced pressure, and in 60 ℃ of vacuum dryings, obtain Semen Ginkgo extrac extractum, and character is: light yellow extractum, be dissolved in alcohol, and water insoluble.
By embodiment 1 Semen Ginkgo extrac nanometer liposome suspension preparation technology, prepare Semen Ginkgo extrac nanometer liposome suspension.
Get above-mentioned Semen Ginkgo extrac nanometer liposome suspension 20ml, by embodiment 3 ointment preparation technologies, prepare Semen Ginkgo extrac nanometer liposome ointment 1000g, Semen Ginkgo extrac nano-lipid body burden is 2%.Be sub-packed in 50 tubules every 20 g.
Embodiment 5:
Folium Ginkgo is shredded, put into pulverizer by its pulverizing.Accurately take a certain amount of Folium Ginkgo through pulverizing, adding solid-liquid ratio is 1:6(W/V) distilled water, at 60 ℃ of bath temperatures, stirring and leaching 2 times, each 4 hours extraction times.After lixiviate finishes, centrifugal (5000 revs/min, 15 minutes), get supernatant.Concentrated supernatant, in 60 ℃ of vacuum dryings, obtains Semen Ginkgo extrac extractum, and character is: light yellow extractum, be dissolved in alcohol, and water insoluble.
By embodiment 1 Semen Ginkgo extrac nanometer liposome suspension preparation technology, prepare Semen Ginkgo extrac nanometer liposome suspension.
Get above-mentioned Semen Ginkgo extrac nanometer liposome suspension 300ml, by embodiment 1 tincture preparation technology, prepare Semen Ginkgo extrac nanometer liposome tincture 1000 ml, Semen Ginkgo extrac nano-lipid body burden is 30%.Be sub-packed in 50 tubules every 20 ml.
Embodiment 6:
Folium Ginkgo is shredded, put into pulverizer by its pulverizing.Accurately take a certain amount of Folium Ginkgo through pulverizing, adding solid-liquid ratio is 1:6(W/V) petroleum ether, at 60 ℃ of bath temperatures, stirring and leaching 2 times, each 4 hours extraction times.After lixiviate finishes, centrifugal (5000 revs/min, 15 minutes), get supernatant.Use vacuum rotary evaporator concentrated supernatant, condition is 55 ℃ of temperature, pressure 0.008-0.009MPa, and concentrating under reduced pressure, and in 60 ℃ of vacuum dryings, obtain Semen Ginkgo extrac extractum, and character is: light yellow extractum, be dissolved in alcohol, and water insoluble.
By embodiment 1 Semen Ginkgo extrac nanometer liposome suspension preparation technology, prepare Semen Ginkgo extrac nanometer liposome suspension.
Get above-mentioned Semen Ginkgo extrac nanometer liposome suspension 200ml, by embodiment 2 gel preparation technologies, prepare Semen Ginkgo extrac nanometer liposome gel 1000 g, Semen Ginkgo extrac nano-lipid body burden is 20%.Be sub-packed in 50 tubules every 20 g.
Embodiment 7:
By embodiment 1 Semen Ginkgo extrac extractum preparation technology, prepare Semen Ginkgo extrac extractum.
By embodiment 1 Semen Ginkgo extrac nanometer liposome suspension preparation technology, prepare Semen Ginkgo extrac nanometer liposome suspension.
Get above-mentioned Semen Ginkgo extrac nanometer liposome suspension 400ml, by embodiment 1 tincture preparation technology, prepare Semen Ginkgo extrac nanometer liposome tincture 1000 ml, Semen Ginkgo extrac nano-lipid body burden is 40%.Be sub-packed in 50 tubules every 20 ml.
Embodiment 8:
By embodiment 2 Semen Ginkgo extrac extractum preparation technologies, prepare Semen Ginkgo extrac extractum.
By embodiment 1 Semen Ginkgo extrac nanometer liposome suspension preparation technology, prepare Semen Ginkgo extrac nanometer liposome suspension.
Get above-mentioned Semen Ginkgo extrac nanometer liposome suspension 1ml, by embodiment 3 ointment preparation technologies, prepare Semen Ginkgo extrac nanometer liposome ointment 1000g, Semen Ginkgo extrac nano-lipid body burden is 0.1%.Be sub-packed in 50 tubules every 20 g.
Embodiment 9:
By embodiment 4 Semen Ginkgo extrac extractum preparation technologies, prepare Semen Ginkgo extrac extractum.
By embodiment 1 Semen Ginkgo extrac nanometer liposome suspension preparation technology, prepare Semen Ginkgo extrac nanometer liposome suspension.
Get above-mentioned Semen Ginkgo extrac nanometer liposome suspension 0.1ml, by embodiment 2 gel preparation technologies, prepare Semen Ginkgo extrac nanometer liposome gel 1000 g, Semen Ginkgo extrac nano-lipid body burden is 0.01%.Be sub-packed in 50 tubules every 20 g.
Embodiment 10:
By embodiment 5 Semen Ginkgo extrac extractum preparation technologies, prepare Semen Ginkgo extrac extractum.
By embodiment 1 Semen Ginkgo extrac nanometer liposome suspension preparation technology, prepare Semen Ginkgo extrac nanometer liposome suspension.
Get above-mentioned Semen Ginkgo extrac nanometer liposome suspension 10ml, by embodiment 2 gel preparation technologies, prepare Semen Ginkgo extrac nanometer liposome gel 1000g, Semen Ginkgo extrac nano-lipid body burden is 1%.Be sub-packed in 50 tubules every 20 g.
The Semen Ginkgo extrac nanometer liposome external preparation of making in order to top method, carries out relevant pharmacology, the experiment of local irritation safety evaluatio and clinic trial test.Experimental data of the present invention below:
To the female rats of the therapeutical effect of rat bacterial vaginitis: body weight 200-250g, from vagina, inject staphylococcus aureus (3 × 10
5cFU/ml) and escherichia coli (1.6 × 10
6cFU/ml) mixed bacteria liquid, bacterium liquid injection rate is 0.05ml/100g body weight, the next day, inoculates 1 time.Within the 5th day and the 7th day, get respectively vaginal secretions and do microscopy, antibacterial culturing, and isolate respectively staphylococcus aureus and escherichia coli, then be inoculated in respectively in culture medium, after cultivating, do identification of strains.Microscopy and identification of strains are positive for infecting successful rat.Get the successful rat of infection and be divided at random 4 groups, model control group, Semen Ginkgo extrac matched group, Semen Ginkgo extrac nanometer liposome group of the present invention, ofloxacin ointment group, 10 every group.In each rat vagina of administration group, inject respectively relative medicine, saline injection in each rat vagina of model control group, every day 1 time, continuous 5 days.Within after drug withdrawal the 1st day and the 4th day, get rat vagina secretions and identify, all negative being decided to be turns out cloudy to identify pathogen for 2 times.Within after drug withdrawal the 5th day, rat is dissected, got vagina specimen and carry out perusal; Vagina specimen is marked by hyperemia, edema, hemorrhage 3 indexs: without hyperemia, edema, hemorrhagely comment 0 point; Vaginal mucosa still smoothly has mild hyperaemia, is chosen as 1 point; Vaginal mucosa is level and smooth moderate hyperemia still, and visible point-like petechia, is chosen as 2 points; Vaginal mucosa is unsmooth, and severe hyperemia is bled profusely a little as seen, organizes Mild edema, is chosen as 3 points.With the score value of model control group, be decided to be 100% (degree of inflammation), medicine group and model control group comparison, can draw medicine group inflammation suppression ratio.With t check, inflammation is marked and added up, use χ
2check is added up negative conversion rate.Result shows (in Table 1), and Semen Ginkgo extrac nanometer liposome gel of the present invention has significant therapeutic effect to rat bacterial vaginitis tool, and effect is significantly better than ofloxacin and Semen Ginkgo extrac external preparation matched group.
The therapeutical effect of table 1. to rat bacterial vaginitis
| Grouping | Inflammation scoring | Inflammation suppression ratio (%) | Negative conversion rate (%) |
| Model contrast | 2.64±0.52 | 0 | 0 |
| Semen Ginkgo extrac | 0.97±0.14 ** | 63.26 | 60 ** |
| Semen Ginkgo extrac nanometer liposome | 0.33±0.09 **## | 87.5 | 80 **# |
| Ofloxacin | 0.95±0.28 ** | 64.02 | 60 ** |
X ± s. n=10. compared with model control group,
* p<0.05,
* p<0.01. compared with Semen Ginkgo extrac group,
# p<0.05,
## p<0.01.
To the female rats of the therapeutical effect of rat colpitis mycotica: body weight 200-250g, the 35 mg/kg anesthesia of lumbar injection pentobarbital sodium, spay.Perform the operation after 10 days, rat skin lower injection estradiol benzoate 10mg/kg, continuous 5 days, vaginal smear under microscope, visible a large amount of keratinocytes, shape is large and irregular, illustrates that rat is in false rutting period.Every 1 estradiol benzoate of injection on the 3rd, make rat maintain false rutting period later.To, in the estrous rat of vacation, from vaginal injection, enter 2.5 × 10
6the Candida albicans 0.1ml of CFU/ml, block vaginal orifice with aseptic cotton balls, prevent that bacterium liquid stream from going out, microscopy vaginal secretions after 10 days, visible rat pudendum is obviously red and swollen, and has a large amount of white secretions, and secretions is inoculated in sabouraud culture medium, after cultivating, microscopy is positive and is the successful rat of infection.Get the successful rat of infection and be divided at random 4 groups, model control group, Semen Ginkgo extrac matched group, Semen Ginkgo extrac nanometer liposome group of the present invention, Miconazole Nitrate Cream group, 10 every group.In each rat vagina of administration group, inject respectively relative medicine, saline injection in each rat vagina of model control group, every day 1 time, continuous 7 days.Before administration in the 4th day and get rat vagina secretions the 8th day morning and cultivate in sabouraud culture medium 48 hours, as without albicans growth for curing, otherwise, do not cure.Use χ
2check is added up cure rate.Result shows (in Table 2), and Semen Ginkgo extrac nanometer liposome ointment of the present invention has significant therapeutic effect to rat colpitis mycotica tool, and effect is significantly better than miconazole nitrate and Semen Ginkgo extrac external preparation matched group.
The therapeutical effect of table 2. to rat colpitis mycotica
| Grouping | Within 3 days, cure (only) | Cure rate (%) | Within 7 days, cure (only) | Cure rate (%) |
| Model contrast | 0 | 0 | 0 | 0 |
| Semen Ginkgo extrac | 4 | 40 * | 7 | 70 ** |
| Semen Ginkgo extrac nanometer liposome | 7 | 70 **# | 10 | 100 **# |
| Miconazole nitrate | 5 | 50 * | 7 | 70 ** |
N=10. compared with model control group,
* p<0.05,
* p<0.01. compared with Semen Ginkgo extrac group,
# p<0.05,
## p<0.01.
The therapeutical effect that human papillomavirus (HPV) is infected: HPV infects positive epithelial cells from male patients with condyloma acuminata patient foreskin position disease damage and women's patients of cervical ruin cervix uteri port part disease damage.Cell culture: trypsin digestion obtains HPV and infects epithelial cells, with 1 × 10
5cell number is inoculated in the 100ml Tissue Culture Flask of coating Mus tail collagen, and condition of culture is 5%CO
2, 37 ℃; Culture medium is RPMI1640 culture fluid, containing 10% calf serum and antibiotics.Use 2.5gL
-1the epithelial cells of trypsinization the 3rd generation monolayer culture, makes single cell suspension, every hole 4 × 10 with culture fluid
4individual cell is inoculated in 24 orifice plates, and every hole adds 1ml culture fluid cellar culture, within 3 days, changes liquid 1 time, continuously 24d.Centrifugal collecting cell, observation of cell form under microscope, and adopt tetramethyl azo azoles salt (MTT) colorimetric determination cell proliferation vigor.Cell is inoculated in to 96 orifice plates, and (every porocyte number is 2 × 10
3), establish Semen Ginkgo extrac matched group, Semen Ginkgo extrac nanometer liposome group of the present invention, interferon group, establish 5 dosage groups for every group, add respectively relative medicine 0,20,40,60,80,100 μ l, each dosage group is established 12 multiple holes; Cultivate after 3 days, sucking-off culture fluid, in every hole, add MTT solution 100 μ l in 37 ℃ of reactions 4 hours, discard MTT, every hole adds 100 μ l DMSO and vibrates 10 minutes, abundant dissolving crystallized thing, 490nm place measures absorbance A value, and experimental result represents (experimental group A value/control group A value × lOO) with cell proliferation rate.Result shows (in Table 3), and compared with blank group, Semen Ginkgo extrac nanometer liposome tincture of the present invention can suppress the propagation of HPV infection cell; The cell that the visible HPV of micro-Microscopic observation infects recovers Normocellular form gradually, illustrate that the present invention has the effect that anti-HPV infects, and effect is significantly better than interferon and Semen Ginkgo extrac external preparation matched group.
The impact of table 3. on the HPV infection cell rate of increase (%)
X ± s. n=12. compared with blank, * *
p<0.01. compared with Semen Ginkgo extrac group,
# p<0.05,
## p<0.01.
To the therapeutical effect of rat cervicitis: set up rat cervicitis model with phenol rubber cement vaginal injection.Phenol adding distil water, Radix Acaciae senegalis are made 25% rubber cement, extract the injection of the capable vagina of 0.15ml deep during modeling, and give self-control medical aseptic cotton silver blocking vaginal orifice, prevent that medicinal liquid from flowing out, and 1 time on the every 3rd, totally 4 times, 12 days.Get the successful rat of modeling and be divided at random 4 groups, model control group, Semen Ginkgo extrac matched group, Semen Ginkgo extrac nanometer liposome group of the present invention, the safe rubber cement group of two azoles, 10 every group.In each rat vagina of administration group, inject respectively relative medicine, saline injection in each rat vagina of model control group, every day 1 time, continuous 7 days.Perusal rat pudendum part and secretions change, and whether vaginal orifice is dry, and mucosa has or not redness, hyperemia, and vagina has or not flow liquid to bleed etc.The pathological change of light Microscopic observation cervical tissue cellularity.Result shows, perusal model group pudendum turns white, and cervix uteri hyperemia, redness have individually dense sexual secretion; The above-mentioned Non Apparent Abnormality of organizing for the treatment of group.The necrosis of micro-Microscopic observation model group cervical mucosal, comes off, and forms ulcer, visible a large amount of cell infiltration under cervical mucosal, little vasodilation, hyperemia, interstitial edema, the visible columnar epithelium squamous metaplasia of squama column intersection; Semen Ginkgo extrac matched group, the necrosis of the safe group of two azoles mucosa, come off, and vasodilation, hyperemia, edema alleviate, and inflammatory cell is less; The above-mentioned performance of Semen Ginkgo extrac nanometer liposome gel group of the present invention obviously alleviates.Illustrate that Semen Ginkgo extrac nanometer liposome gel of the present invention has significant therapeutic effect to rat cervicitis tool, can promote wound tissue healing, curative effect is better than the two calm and peaceful Semen Ginkgo extrac external preparation of azoles matched groups.
Local irritation test: get 8 of adult rabbit, male and female half and half, every animal sub-cage rearing, first raises 3-4 days at laboratory condition.Then rabbit spinal column both sides dorsal body setae is shaved off to every lateral area 5 × 10cm with electric shaver-for women
2.Carefully check that whether depilation district has redness and damage, if any abandoning it, can not test.Adopt the test method of multiple dosing, in depilation, Semen Ginkgo extrac nanometer liposome external preparation of the present invention is smeared in district, every day 2 times, smears continuously 7 days.Whether last is smeared after 24 hours and is washed away smear with warm water, observes local skin reaction respectively at l, after 24,48,72 hours, as erythema, edema, desquamation, incrustation etc., have petechia, skin peptide coarse or poor etc., records time of origin and regression time.Experimental result shows, is smearing continuously Semen Ginkgo extrac nanometer liposome external preparation of the present invention after 7 days, and each rabbit skin does not all show obvious skin irritation and anaphylaxis.Show product safety of the present invention, have no adverse reaction.
Clinic trial test: the Semen Ginkgo extrac nanometer liposome external preparation of making in order to top method, on probation through multiple gynecological patient, concrete case and the course for the treatment of are in Table 4.All patients are coated with disease damage place outward with the present invention, every day 1 time.Patient does not all use any other external used medicine during treating.Criterion of therapeutical effect: cure: clinical symptoms performance all disappears, and pathogen microscopy is negative; Effective: clinical symptoms performance is basic to disappear, and pathogen microscopy is the weak positive; Effective: clinical symptoms performance part disappears, and pathogen microscopy is the weak positive; Invalid: clinical symptoms shows constant or increases the weight of, and pathogen microscopy is positive.Cure rate=healing number/total case X100%; Effective percentage=(healing number+effective number+efficiently individual quantity)/total case X100%.The results are shown in Table 4.Clinic trial result shows, multiple gynecological patient uses Semen Ginkgo extrac nanometer liposome external preparation of the present invention all effective, and effective percentage reaches 100%; Cure rate all exceedes 80%.During application the present invention, all there is not the untoward reaction such as skin irritation, allergy in all patients.Therefore the present invention is the gynopathic external preparation of a kind of desirable treatment, evident in efficacy, safe and reliable.
Table 4 Semen Ginkgo extrac nanometer liposome of the present invention external preparation is treated gynopathic clinical effectiveness
The present invention is compared with prior art: the present invention derives from natural precious medicinal plants Semen Ginkgo, from positions such as Folium Ginkgo, fruits, extract and obtain Semen Ginkgo extrac, take Semen Ginkgo extrac as effective ingredient, make nanometer liposome external preparation, the sick tool of multiple gynecological is had significant therapeutic effect, and curative effect is better than common antibiotics or immunosuppressant, without the side effect due to life-time service immunosuppressant, antibiotic.Therefore Semen Ginkgo extrac nanometer liposome external preparation of the present invention curative effect aspect treatment gynaecopathia is outstanding, uses safety to have no side effect, and has good potential applicability in clinical practice.
Claims (7)
1. the purposes of Semen Ginkgo extrac nanometer liposome in the gynopathic external preparation of preparation control.
2. purposes as claimed in claim 1, described gynaecopathia is trichomonal vaginitis, colpitis mycotica, bacterial vaginitis, pudendal pruritus, cervicitis, cervical erosion.
3. Semen Ginkgo extrac nanometer liposome as claimed in claim 1, described Semen Ginkgo extrac is by Chemical Decomposition method, and from the leaf of Semen Ginkgo, really, obtain the extract at rhizome position, extraction solvent is ethanol, water, ether, petroleum ether organic solvent; Or to Folium Ginkgo, really, rhizome extracts elaboration products and in remaining waste material, again reclaims extracting in the course of processing; Described Semen Ginkgo extrac nanometer liposome, by adopting the preparation of thin-film ultrasonic-high pressure homogenization method.
4. external preparation as claimed in claim 1 is the gynopathic medicine for the treatment of, cosmetics or the disinfectant external preparation that contain Semen Ginkgo extrac nanometer liposome; And comprise Semen Ginkgo extrac nanometer liposome and any combinations of substances is used for the treatment of gynopathic medicine, cosmetics or disinfectant external preparation; It is characterized in that, described preparation is said exterior-applied formulation on any pharmaceutics, is liquid preparation, cream cream, gel, powder, membrane, lotion, aerosol spray, transdermal absorption formulation, subcutaneous injection agent.
5. medicine as claimed in claim 4, cosmetics or disinfectant external preparation, is characterized in that, contains Semen Ginkgo extrac nanometer liposome 0.001-80%.
6. medicine as claimed in claim 4, cosmetics or disinfectant external preparation, is characterized in that, contains Semen Ginkgo extrac nanometer liposome 0.001-30%.
7. medicine as claimed in claim 4, cosmetics or disinfectant external preparation, is characterized in that, contains Semen Ginkgo extrac nanometer liposome 0.5-20%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104857035A (en) * | 2015-04-24 | 2015-08-26 | 天益京（天津）生物科技有限公司 | Ginkgo biloba leaf extract preparation method |
| CN106177475A (en) * | 2016-09-26 | 2016-12-07 | 贵州柏泰合生物科技有限公司 | A kind of Chinese medicine composition for gynaecopathia |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102302524A (en) * | 2011-08-18 | 2012-01-04 | 王青 | Applications of gingko extractive in preparing external preparation for treating venereal disease, gynecopathy and perianal diseases |
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Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102302524A (en) * | 2011-08-18 | 2012-01-04 | 王青 | Applications of gingko extractive in preparing external preparation for treating venereal disease, gynecopathy and perianal diseases |
Non-Patent Citations (1)
| Title |
|---|
| 江彬彬等: "脂质体透皮吸收的研究进展", 《中国药房》, vol. 13, no. 6, 31 December 2002 (2002-12-31), pages 369 - 371 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104857035A (en) * | 2015-04-24 | 2015-08-26 | 天益京（天津）生物科技有限公司 | Ginkgo biloba leaf extract preparation method |
| CN106177475A (en) * | 2016-09-26 | 2016-12-07 | 贵州柏泰合生物科技有限公司 | A kind of Chinese medicine composition for gynaecopathia |
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