CN103749957B - Preparation method for blue-green algae single-cell protein feed - Google Patents
Preparation method for blue-green algae single-cell protein feed Download PDFInfo
- Publication number
- CN103749957B CN103749957B CN201310749678.5A CN201310749678A CN103749957B CN 103749957 B CN103749957 B CN 103749957B CN 201310749678 A CN201310749678 A CN 201310749678A CN 103749957 B CN103749957 B CN 103749957B
- Authority
- CN
- China
- Prior art keywords
- blue
- green algae
- medium
- black
- fermentation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention provides a preparation method for a blue-green algae single-cell protein feed. According to the preparation method, aspergillus niger with the preservation number of CGMCC (China General Microbiological Culture Collection Center) No.8640 is used for carrying out solid-state fermentation on blue-green algae. The preparation method comprises the specific steps: (1) dehydrating the blue-green algae and carrying out filter pressing to prepare a blue-green algae dehydrated filtering block; (2) inoculating aspergillus niger liquid into a solid-state culture medium and culturing to obtain solid-state aspergillus niger; (3) adding the solid-state aspergillus niger into the blue-green algae dehydrated filtering block and mixing and fermenting to obtain a blue-green algae fermentation product; (4) drying the blue-green algae fermentation product by hot air, and crushing and sieving to obtain the blue-green algae single-cell protein feed. According to the preparation method for the blue-green algae single-cell protein feed, waste resources are utilized, the dehydration is energy-saving and the environmental pollution is not caused; the detoxification of micro-capsule blue-green algae toxin and the synchronous production of the single-cell protein feed are realized.
Description
Technical field
The present invention relates to a kind of waste resource fodder and utilize technology, specifically a kind of preparation method of blue-green algae single-cell protein feed.
Technical background
Along with industrial and agricultural production continues develop rapidly, the frequent aggravation of mankind's activity, cause inland-lake pool pollution condition and be on the rise, the rudimentary plant blue-green algae in lake can overgrow in 6 annual-October, is referred to as " wawter bloom "." wawter bloom " takes place frequently, and to local society economic development, life and the life and health of resident bring adverse influence, it multiple, and continuation and seriousness have caused the very big concern of government and society all circles.
Blue-green algae is planted at the frequent species of China nearly more than 300, wherein dominant species name is called microcystic aeruginosa (Microcystisaeruyinvsa), when in the inland lakes such as Taihu Lake, Chaohu and Dian Chi, " wawter bloom " occurs, occupation rate is about more than 95%, and almost full lake is all this absolute predominance population.The Microcystin (Microcystin is called for short MC) that micro-capsule blue-green algae produces is that one has hepatotoxicity wind agitation and short carcinous cyclic amino acid peptide matters.Report according to the study, Microcystin MC can cause fish poisoning, and in heavy dose of situation, severe patient can lethally be died, and Microcystin MC can cause hepatic injury to mammal small white mouse, causes hepatomegaly and liver tissue lesions.But also there is research to point out, Microcystin MC does not show MA to mouse bone marrow cells chromosome, does not also find its aberration inducing effect, at least can say, Microcystin MC, to Mammalian genetic material DNA, also lacks the abundant foundation of Mutagenicity and MA.
The annual blue-green algae that micro-capsule cyanobacterial bloom produces in lake, biomass is huge.Only jiangsu wuxi one city statistics, the blue-green algae liquid (wherein containing blue-green algae dry 1-2%) salvaging out from 2011 ~ in June, 2013-October from Taihu Lake has nearly 1,000,000 tons more than every year, and this is only a part for blue-green algae generation in lake.How to process and make good use of these blue algae resources, it is turned waste into wealth, there is great Social Ecology benefit and economic implications.
By the analysis to Taihu Lake Microcystis aeruginosa resource, wherein nitrogenous about about 10%, phosphorous close to 1%, the crude protein content that measure and calculation obtains is estimated about 50%, blue algae polysaccharide 5%, crude fat, close to 0.3%, also containing natural colouring matter, unrighted acid and cyanophycean toxin, contains varied physiologically active components such as antibacterial, antiviral and anticancer in addition.Micro-capsule blue-green algae nutrition rich connotation, physiologically active presents many-sided function, and resources development and utilization is well worth doing.
At present the industrialization compared with wide-range is not yet entered to the exploitation of micro-capsule blue-green algae, except by marsh gas power generation can be produced the anaerobic fermentation of blue-green algae and produce except organic fertilizer, be mostly also in the experimental exploring stage, also do not reach plant-scale extensive utilization.Applicant thinks, blue-green algae is made protein feed, and significant is paid close attention to, significant:
The first, micro-capsule blue-green algae is produced protein feed, and the resources advantage of itself can farthest be played, and its nitrogenous height, cyanophycin are abundant, and kind is varied.Once had report, grinds after blue-green algae drying, compared by blue algae powder with other high-quality protein resources, blue algae powder albumen is 1.34 ~ 1.46 times of soybean protein, is 3.5 ~ 4.1 times of milk powder, be eel, 4.5 ~ 5.8 times of soft-shelled turtle powder.
Second, the feeding effect of blue-green algae to livestock and poultry and aquatic livestock is remarkable, from blue algae powder make an addition to carry out feeding trial feed effect viewed from, with the addition of 4 ~ 6% blue algae powders in pork pig basal feed, 18 ~ 40% can be increased weight, add in layer diets by 0.1 ~ 1% blue algae powder, laying rate can improve 3.5% ~ 5.3%.Blue-green algae is applied to aquaculture, breeds fish with making bait containing 5% blue algae powder, compared with the control, carp can be made to increase weight 20.1%, crucian weightening finish 18.3%, bream weightening finish 10.6%.Separately there is bibliographical information, the broiler chicken of blue algae powder of feeding, significantly can improve the appearance luster of chicken leg and sternal rib, improve outward appearance and the class of product.
3rd, the shortage of protein feed is the development bottleneck of feed industry and aquaculture for many years.According to statistics, the whole nation provides the artifical compound feed such as livestock and poultry and aquatic livestock to reach 1.3 hundred million tons/year, is equivalent to 1/4 ~ 1/5 of National Grain.If realize blue-green algae being made " without grain protein feed " considerable grain can be saved.The innovative development of this feed technology and popularization, contribute to the problem alleviating current China protein feed deficiency.
But the micro-capsule blue-green algae utilizing lake outburst " wawter bloom " to produce makes cyanophycin feed, mainly contains two large technical problems: one is how effectively to utilize micro-capsule blue-green algae Middle nutrition component, transform and produce cyanophycin feed; Two is how effectively to remove micro-capsule cyanophycean toxin MC.
Existing technology is also in laboratory scale experimental stage, is difficult to form industrialization.Substantially be all first by physics or chemical method, under intense conditions (high temperature, strong acid, highly basic), remove cyanophycean toxin, then make feed.For example, physical method (Chinese invention patent " processing method of blue-green algae ", publication number CN102150747A) is adopted to be by 150 ~ 300 DEG C, 10 ~ 60min high-temperature process detoxification element, obtain blue-green algae dry powder by≤60 DEG C of dryings, it is said and can be used as feed addictive.This method major defect is that treatment temperature is too high, has destruction to protein peptide bond structure, can affect the quality of forage protein; Meanwhile, adopt temperature high like this to remove cyanophycean toxin also undesirable, high temperature 240 DEG C process 1 hour, MC-LR is also surplus 53.19ug/kg.Again for example, adopt chemical method (Chinese invention patent " a kind of preparation method of detoxicated algae ", publication number CN1268317A) first blue-green algae is made dry algae powder, then adopt 1 ~ 3% high-concentration ozone water to carry out cyanophycean toxin detoxification, or by ozone-containing air (O
3content is 20%) carry out cyanophycean toxin detoxification.But herein to the method for these the two kinds of ozone detoxifications proposed, whether concrete effect how, do not disclose concrete data in file, as the product after detoxification, can adopt, be not also described further for feed.Again for example, blue-green algae hydrolysis is obtained amino acid whose chemical method (Chinese invention patent " bloom blue algae hydrolysis obtains the method for free amino acid ", publication number CN101133778) rich blue algae water (water content 75 ~ 97%) is added the concentrated sulfuric acid (or concentrated hydrochloric acid) in reaction pot, first hydrolysis 5 ~ 8 hours in 100 ~ 120 DEG C, add zinc salt or mantoquita again as catalyst, hydrolysis 2 ~ 5 hours again, alkali neutralization is added after cooling, filter and remove precipitation, supernatant hydrolyzate adopts spraying dry or vacuum drying or freeze drying to obtain amino-acid powder.But, in strong acid, high temperature, hydrolysis can destroy the tryptophan in protein composition, also make cystine and the oxidation of methionine generating portion, and by the hydrolyzate convection drying after neutralization, volume is very large, not only the salt content of product is high, and production cost is very high, feed circle and aquaculture popularization beyond affordability.
Summary of the invention
For above-mentioned technical barrier, the invention provides a kind of microorganism solid fermentation method, synchronously realize the detoxification of micro-capsule cyanophycean toxin and form the two-way object of single-cell protein feed, the object of the present invention is achieved like this:
A preparation method for blue-green algae single-cell protein feed, is characterized in that, be that the black-koji mould (Aspergillusniger) of CGMCCNo.8640 carries out solid state fermentation to blue-green algae for zymophyte with preserving number, concrete steps are as follows:
1) the blue algae dewatering filter block that water content is 79%-87.2% is made in blue algae dewatering, press filtration;
2) be 1 × 10 by concentration
6-1 × 10
8the black-koji mould liquid of cfu/ml by volume mass ratio 3%-5% is inoculated in solid medium, in 28-35 DEG C, cultivates 36-48h, obtain solid-state black-koji mould under relative humidity 85-90 ﹪ environment;
Wheat bran and the water of described solid state rheology based formulas to be mass ratio be 1-1.5;
3 ﹚ add auxiliary material in blue algae dewatering filter block described in step 1, form the blue-green algae culture medium that water content is 60%, in blue-green algae culture medium, add the solid-state black-koji mould in step 2, form fermentation medium, the mass ratio of described solid-state black-koji mould and blue-green algae culture medium is 4%-6%; Being mixed by fermentation medium is placed in koji tray, in 30-37 DEG C, the environment bottom fermentation 36-48h of relative humidity 85-90%, obtains blue algae fermentation product;
The blue algae fermentation product that step 3 obtains by 4 ﹚, by the heated-air drying of 80-100 DEG C, makes its water content < 10%, then pulverizes and sieves, and namely obtains blue-green algae single-cell protein feed.
In the present invention, described black-koji mould liquid obtains like this:
Black-koji mould streak inoculation is activated on Czapek's medium, 28-30 DEG C of lower inclined plane cultivates picking colony after 3-5 days, then streak inoculation is in Kolle flask Czapek's medium, and 28-30 DEG C of lower inclined plane spreads cultivation 3-4 days, then add sterilized water scraping inclined-plane bacterium colony and make bacteria suspension, as black-koji mould liquid;
Or black-koji mould streak inoculation is activated on Czapek's medium, 28-30 DEG C of lower inclined plane cultivates picking colony after 3-5 days, streak inoculation is in Kolle flask Czapek's medium again, 28-30 DEG C of lower inclined plane spreads cultivation 3-4 days, then add after sterilized water scraping inclined-plane bacterium colony makes bacteria suspension, be inoculated in triangle shaking flask fluid nutrient medium with volume ratio 3-5%, 30-32 DEG C, 150-180rpm. shaker fermentation is cultivated 40-50h and is obtained shaking table bacterium liquid, as black-koji mould liquid;
Or black-koji mould streak inoculation is activated on Czapek's medium, 28-30 DEG C of lower inclined plane cultivates picking colony after 3-5 days, streak inoculation is in Kolle flask Czapek's medium again, 28-30 DEG C of lower inclined plane spreads cultivation 3-4 days, then add after sterilized water scraping inclined-plane bacterium colony makes bacteria suspension, be inoculated in triangle shaking flask fluid nutrient medium with volume ratio 3-5%, 30-32 DEG C, 150-180rpm. shaker fermentation is cultivated 40-50h and is obtained shaking table bacterium liquid, be inoculated in seeding tank fluid nutrient medium with volume ratio 5-10 ﹪ shaking table bacterium liquid, logical filtrated air stirs, zymocyte liquid is obtained at 30-32 DEG C of fermented and cultured 36-48h, as black-koji mould liquid,
Wherein, Czapek's medium formula is: in 1L distilled water, add 3g NaNO
3, 1g K
2hPO
4, 0.5g KCL, 0.5gMgSO
4﹒ 7H
2o, 0.01g FeSO
4﹒ 4H
2o, 30g sucrose and 15-20g agar, adjustment pH is 6.0-6.5;
Triangle shaking flask fluid nutrient medium and seeding tank Liquid Culture based formulas are: in 1L distilled water, add 3g NaNO
3, 1gK
2hPO
4, 0.5g KCL, 0.5g MgSO
4﹒ 7H
2o, 0.01g FeSO
4﹒ 4H
2o and 30g sucrose, adjustment pH is 6.0-6.5.
In the present invention, described auxiliary material is wheat bran, or blue algae powder, or powder of straw or the combination between them.
The present invention adopts microorganism solid fermentation method, particularly have employed unsterilised Solid Fermentation of Uncooked Material technology, synchronously carries out removing cyanophycean toxin MC and transforming the technical process that blue-green algae is single-cell protein feed two aspects.The biological oxidation produced by fermenting on the one hand, metabolism assimilation, and the catalyticing decomposition action that enzymatic production is further produced, define a series of resultant effects such as the open loop to micro-capsule cyanophycean toxin, decomposition and utilization, cause Algae toxins to be constantly decomposed during the fermentation, destroy and utilize, constantly removed; On the other hand simultaneously, fermentation also makes the diversified nutrition composition in blue-green algae obtain to reconfigure and recycle, zymophyte self is bred rapidly, the unicellular organism thalline that quantity of formation is huge and precursor thereof, namely become blue-green algae single cell protein, thus realize the fodder of blue-green algae.
The invention has the advantages that: 1, solid state fermentation conditions is natural, ecological, gentle, tunning enriches, without micro-capsule cyanophycean toxin MC, except results mycoprotein, also can produce multiple digestive ferment, organic acid, vitamin and natural antimicrobial substance, and the somatomedin of multiple the unknown, the blue-green algae forage protein nutrition formed is good, inherent quality is high; 2, the dehydration energy saving effect of highly significant is presented in solid state fermentation production process, and non-environmental-pollution, be again utilize waste resource, make low cost product, be conducive to applying in various cultivation industry; 3, solid state fermentation ratio is easier to realize industrial large-scale production, can utilize blue algae resource in a large number.
Detailed description of the invention
In conjunction with specific embodiments, illustrate the present invention further, these embodiments should be understood and be only not used in for illustration of the present invention and limit the scope of the invention.
The preparation of embodiment 1 black-koji mould
The black-koji mould that the application adopts derives from Institute of Microorganism, Academia Sinica, applicant by this bacterial strain is carried out blue-green algae domestication adapt to after, what the strain obtained was new has lifting single cell protein, improve the black-koji mould of fermentation dehydration rate and anti-living contaminants, this new Aspergillus niger strain is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center on December 27th, 2013 by applicant, address is: No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City, Institute of Microorganism, Academia Sinica, postcode: 100101, preserving number is CGMCCNo.8640.
The preparation of embodiment 2 blue algae dewatering filter block
Water content blue algae collecting liquid more than 98% is carried out pressure floatation air by pressurized dissolved air flotation machine, then uses sludge filter press press filtration, obtained water content is the blue algae dewatering filter block of 79%-87.2%.
Embodiment 3 blue-green algae solid state fermentation prepares single-cell protein feed
Culture medium involved by the present embodiment:
Wheat bran and the water of solid state rheology based formulas to be mass ratio be 1-1.5;
Czapek's medium is filled a prescription: in 1L distilled water, add 3g NaNO
3, 1g K
2hPO
4, 0.5g KCL, 0.5g MgSO
4﹒ 7H
2o, 0.01g FeSO
4﹒ 4H
2o, 30g sucrose and 15-20g agar, adjustment pH is 6.0-6.5.
What Example 1 obtained activates black-koji mould streak inoculation on Czapek's medium, 28-30 DEG C of lower inclined plane cultivates 3-5 days, cover with after bacterium colony until inclined-plane, picking colony, streak inoculation is in Kolle flask Czapek's medium, and 28-30 DEG C of lower inclined plane spreads cultivation 3-4 days, treat that inclined-plane covers with bacterium colony, adding sterilized water scraping inclined-plane bacterium colony and make bacteria suspension, as black-koji mould liquid, is 1 × 10 by 3ml concentration
6-1 × 10
8the black-koji mould liquid of cfu/ml is inoculated in (volume mass ratio is 3%) in 100g solid medium, 28-35 DEG C, cultivate 36-48h under relative humidity 85-90 ﹪ environment, obtain solid-state black-koji mould.
Obtaining water content with method described in embodiment 2 is 87.2% dehydration filter block 500g, add 312g wheat bran, blue-green algae moisture content in medium is made to adjust to about 60%, add the solid-state black-koji mould of 32.5g (mass ratio is about 4%) again, mix, obtain in granular fermentation medium, fermentation medium is layered in ventilative koji tray, thickness is 4cm, temperature 35 DEG C, relative humidity 86%, natural ventilation oxygen environment bottom fermentation 48h, fermentation medium all can form the tunning of mycelium enclosed mass, by tunning by pulverizing and sieving after the heated-air drying of 80-100 DEG C, namely blue-green algae single-cell protein feed 299g is obtained, crude protein content 27.6% is recorded by Kjeldahl's method.
Embodiment 4 blue-green algae solid state fermentation removes Algae toxins
Culture medium involved by the present embodiment:
Wheat bran and the water of solid state rheology based formulas to be mass ratio be 1-1.5;
Czapek's medium is filled a prescription: in 1L distilled water, add 3g NaNO
3, 1g K
2hPO
4, 0.5g KCL, 0.5g MgSO
4﹒ 7H
2o, 0.01g FeSO
4﹒ 4H
2o, 30g sucrose and 15-20g agar, adjustment pH is 6.0-6.5;
Triangle shaking flask Liquid Culture based formulas is: in 1L distilled water, add 3g NaNO
3, 1g K
2hPO
4, 0.5g KCL, 0.5gMgSO
4﹒ 7H
2o, 0.01g FeSO
4﹒ 4H
2o and 30g sucrose, adjustment pH is 6.0-6.5.
The aspergillus niger strain streak inoculation that Example 1 obtains activates on Cha Shi slant medium, 28-30 DEG C of lower inclined plane cultivates 3-5 days, picking colony after bacterium colony is covered with until inclined-plane, streak inoculation is the 28-30 DEG C of 3-4 days that spreads cultivation on Kolle flask inclined-plane, treat that inclined-plane covers with bacterium colony, add sterilized water scraping inclined-plane bacterium colony and make bacteria suspension, being about 3-5% with volume ratio is inoculated in triangle shaking flask fluid nutrient medium, 30-32 DEG C, 150-180rpm. shaker fermentation cultivation 40-50h obtains the streak inoculation of shaking table strain liquid black-koji mould and activates on Czapek's medium, 28-30 DEG C of lower inclined plane cultivates picking colony after 3-5 days, streak inoculation is in Kolle flask Czapek's medium again, 28-30 DEG C of lower inclined plane spreads cultivation 3-4 days, then add after sterilized water scraping inclined-plane bacterium colony makes bacteria suspension, be inoculated in triangle shaking flask fluid nutrient medium with volume ratio 3-5%, 30-32 DEG C, 150-180rpm. shaker fermentation is cultivated 40-50h and is obtained shaking table bacterium liquid, as black-koji mould liquid, be 1 × 10 by 4ml concentration
6-1 × 10
8the black-koji mould liquid of cfu/ml is inoculated in (volume mass ratio is 4%) in 100g solid medium, 28-35 DEG C, cultivate 36-48h under relative humidity 85-90 ﹪ environment, obtain solid-state black-koji mould.
The blue algae dewatering filter block of 1000g water content 80.8% is obtained with method described in embodiment 2, add powder of straw 100g wherein, wheat bran 304g, its water content is to about 60%, then add after 70.2g solid black Aspergillus (mass ratio is about 5%) fully mixes and obtain fermentation medium, the fermentation medium taking out half amount is dried as fermentation medium dry powder immediately, in celadon (contrast as before fermentation).Second half fermentation medium adopts tray to leave standstill solid state fermentation, be paved into thickness 4cm, temperature 35 DEG C, relative humidity more than 86%, fermentation 48h, fermentation medium forms the tunning of mycelia enclosed mass, and tunning is obtained tunning dry powder 285g by the heated-air drying of 80-100 DEG C, in yellowish-brown, record crude protein content 38.7% by Kjeldahl's method.Send Southern Yangtze University's Food Science and analysis center of technology National Key Laboratory to test the content detecting Algae toxins (MC-LR, MC-YR and MC-RR) in the lump in fermentation medium dry powder (before fermentation sample) and tunning dry powder (after fermenting sample), test data is as shown in table 1: the virus elimination rate (%) calculating toxin:
Sample content of toxins contrast before and after table 1 ferments
From table 1 result, the virus elimination rate of MC-LR and MC-RR two kinds of toxin kinds is all more than 70%, and MC-YR tunning does not detect, and virus elimination rate reaches 100%.Applicant thinks, the application adopts the detoxification efficiency of black-koji mould solid state fermentation to micro-capsule blue-green algae remarkable.
The blue algae dewatering filter block of embodiment 5 different moisture content is on the impact of tunning
Culture medium involved by the present embodiment:
Wheat bran and the water of solid state rheology based formulas to be mass ratio be 1-1.5;
Czapek's medium is filled a prescription: in 1L distilled water, add 3g NaNO
3, 1g K
2hPO
4, 0.5g KCL, 0.5g MgSO
4﹒ 7H
2o, 0.01g FeSO
4﹒ 4H
2o, 30g sucrose and 15-20g agar, adjustment pH is 6.0-6.5.
Triangle shaking flask fluid nutrient medium and seeding tank Liquid Culture based formulas are: in 1L distilled water, add 3g NaNO
3, 1g K
2hPO
4, 0.5g KCL, 0.5g MgSO
4﹒ 7H
2o, 0.01g FeSO
4﹒ 4H
2o and 30g sucrose, adjustment pH is 6.0-6.5.
The black-koji mould streak inoculation that Example 1 obtains activates on Czapek's medium, 28-30 DEG C of lower inclined plane cultivates picking colony after 3-5 days, streak inoculation is in Kolle flask Czapek's medium again, 28-30 DEG C of lower inclined plane spreads cultivation 3-4 days, then add after sterilized water scraping inclined-plane bacterium colony makes bacteria suspension, be inoculated in triangle shaking flask fluid nutrient medium with volume ratio 3-5%, 30-32 DEG C, 150-180rpm. shaker fermentation is cultivated 40-50h and is obtained shaking table bacterium liquid, be inoculated in seeding tank fluid nutrient medium with volume ratio 5-10 ﹪ shaking table bacterium liquid, logical filtrated air stirs, zymocyte liquid is obtained at 30-32 DEG C of fermented and cultured 36-48h, as black-koji mould liquid, be 1 × 10 by 5ml concentration
6-1 × 10
8the black-koji mould liquid of cfu/ml is inoculated in (volume mass ratio is 5%) in 100g solid medium, 28-35 DEG C, cultivate 36-48h under relative humidity 85-90 ﹪ environment, obtain solid-state black-koji mould.
Obtain water content with method described in embodiment 2 and be respectively 87.2%, 86.5%, 84.4%, 79%, 81.5%, the each 500g of dehydration filter block of 80.8%, numbering 1-6 group respectively, adding wheat bran wherein is respectively 312g, 300g, 277g, 215g, 244g and 236g, moisture content in medium is made to adjust to about 60%, add 48.7g respectively again, 48.0g, 46.6g, 42.9g, the solid-state aspergillus niger (mass ratio is about 6%) of 44.6g and 44.2g, mix, obtain in granular fermentation medium, fermentation medium is layered in ventilative koji tray, thickness is 4cm, temperature 35 DEG C, relative humidity 86%, natural ventilation oxygen environment bottom fermentation 48h, fermentation medium all can form the tunning of mycelium enclosed mass, by tunning by pulverizing and sieving after the heated-air drying of 80-100 DEG C, the crude protein content of tunning is recorded by Kjeldahl's method, result is as shown in table 2:
Table 2 different moisture content blue algae dewatering filter block fermentation after crude protein content
From table 2 result, the protein content of tunning becomes negative correlation with the water content of blue algae dewatering filter block before fermentation, by reducing the water content of filter block, can improve the protein content of product after solid state fermentation.
The above is only the preferred embodiment of the present invention, it should be pointed out that for those skilled in the art, under the premise without departing from the principles of the invention, can also make some improvement, and these improvement also should be considered as protection scope of the present invention.
Claims (3)
1. a preparation method for blue-green algae single-cell protein feed, is characterized in that, with preserving number be CGMCC No.8640 black-koji mould (
aspergillus niger) for zymophyte carries out solid state fermentation to blue-green algae, concrete steps are as follows:
1) the blue algae dewatering filter block that water content is 79%-87.2% is made in blue algae dewatering, press filtration;
2) be 1 × 10 by concentration
6-1 × 10
8the black-koji mould liquid of cfu/ml by volume mass ratio 3%-5% is inoculated in solid medium, in 28-35 DEG C, cultivates 36-48h, obtain solid-state black-koji mould under relative humidity 85-90 ﹪ environment;
Wheat bran and the water of described solid state rheology based formulas to be mass ratio be 1-1.5;
3 ﹚ add auxiliary material in blue algae dewatering filter block described in step 1, form the blue-green algae culture medium that water content is 60%, in blue-green algae culture medium, add the solid-state black-koji mould in step 2, form fermentation medium, the mass ratio of described solid-state black-koji mould and blue-green algae culture medium is 4%-6%; Being mixed by fermentation medium is placed in koji tray, in 30-37 DEG C, the environment bottom fermentation 36-48h of relative humidity 85-90%, obtains blue algae fermentation product;
The blue algae fermentation product that step 3 obtains by 4 ﹚, by the heated-air drying of 80-100 DEG C, makes its water content < 10%, then pulverizes and sieves, and namely obtains blue-green algae single-cell protein feed.
2. the preparation method of blue-green algae single-cell protein feed according to claim 1, is characterized in that described black-koji mould liquid obtains like this:
Black-koji mould streak inoculation is activated on Czapek's medium, 28-30 DEG C of lower inclined plane cultivates picking colony after 3-5 days, then streak inoculation is in Kolle flask Czapek's medium, and 28-30 DEG C of lower inclined plane spreads cultivation 3-4 days, then add sterilized water scraping inclined-plane bacterium colony and make bacteria suspension, as black-koji mould liquid;
Or black-koji mould streak inoculation is activated on Czapek's medium, 28-30 DEG C of lower inclined plane cultivates picking colony after 3-5 days, streak inoculation is in Kolle flask Czapek's medium again, 28-30 DEG C of lower inclined plane spreads cultivation 3-4 days, then add after sterilized water scraping inclined-plane bacterium colony makes bacteria suspension, be inoculated in triangle shaking flask fluid nutrient medium with volume ratio 3-5%, 30-32 DEG C, 150-180rpm. shaker fermentation is cultivated 40-50h and is obtained shaking table bacterium liquid, as black-koji mould liquid;
Or black-koji mould streak inoculation is activated on Czapek's medium, 28-30 DEG C of lower inclined plane cultivates picking colony after 3-5 days, streak inoculation is in Kolle flask Czapek's medium again, 28-30 DEG C of lower inclined plane spreads cultivation 3-4 days, then add after sterilized water scraping inclined-plane bacterium colony makes bacteria suspension, be inoculated in triangle shaking flask fluid nutrient medium with volume ratio 3-5%, 30-32 DEG C, 150-180rpm. shaker fermentation is cultivated 40-50h and is obtained shaking table bacterium liquid, be inoculated in seeding tank fluid nutrient medium with volume ratio 5-10 ﹪ shaking table bacterium liquid, logical filtrated air stirs, zymocyte liquid is obtained at 30-32 DEG C of fermented and cultured 36-48h, as black-koji mould liquid,
Wherein, Czapek's medium formula is: in 1L distilled water, add 3g NaNO
3, 1g K
2hPO
4, 0.5g KCL, 0.5g MgSO
4﹒ 7H
2o, 0.01g FeSO
4﹒ 4H
2o, 30g sucrose and 15-20g agar, adjustment pH is 6.0-6.5;
Triangle shaking flask fluid nutrient medium and seeding tank Liquid Culture based formulas are: in 1L distilled water, add 3g NaNO
3, 1g K
2hPO
4, 0.5g KCL, 0.5g MgSO
4﹒ 7H
2o, 0.01g FeSO
4﹒ 4H
2o and 30g sucrose, adjustment pH is 6.0-6.5.
3. the preparation method of blue-green algae single-cell protein feed according to claim 1 or 2, it is characterized in that, described auxiliary material is wheat bran, or blue algae powder, or powder of straw, or the combination between them.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310749678.5A CN103749957B (en) | 2013-12-31 | 2013-12-31 | Preparation method for blue-green algae single-cell protein feed |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310749678.5A CN103749957B (en) | 2013-12-31 | 2013-12-31 | Preparation method for blue-green algae single-cell protein feed |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103749957A CN103749957A (en) | 2014-04-30 |
| CN103749957B true CN103749957B (en) | 2015-05-20 |
Family
ID=50517412
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310749678.5A Active CN103749957B (en) | 2013-12-31 | 2013-12-31 | Preparation method for blue-green algae single-cell protein feed |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103749957B (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106617043A (en) * | 2016-09-22 | 2017-05-10 | 苏州太阳都生化技术有限公司 | Preparation method and application of bloom cyanobacteria nutrient solution |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101215539B (en) * | 2008-01-07 | 2010-06-02 | 周文彩 | Blue algae biological bacterium leaven, nutriment and biological organic fertilizer containing same, and preparing method thereof |
| CN101317625B (en) * | 2008-06-27 | 2011-12-07 | 刘新虎 | Comprehensive treatment method for pollution of livestock and poultry aquaculture, special feedstuff bridging agent, biological edible warm pad and biological organic fertilizer |
| CN101653189B (en) * | 2009-08-26 | 2012-04-25 | 四川国凤生物科技有限公司 | Natural plant small molecular group substance concentrate and production method thereof |
| CN101849618A (en) * | 2010-04-13 | 2010-10-06 | 浙江大学 | Method for producing complex enzyme for feed |
| CN102132762B (en) * | 2011-03-29 | 2013-01-23 | 南开大学 | Straw feed and preparation method thereof |
-
2013
- 2013-12-31 CN CN201310749678.5A patent/CN103749957B/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| CN103749957A (en) | 2014-04-30 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103478413B (en) | Method for producing protein feed by mixed-strain solid-state fermentation of ginkgo leaf residues | |
| CN103468594B (en) | Candidautilis strain and application thereof | |
| CN109497265A (en) | It is a kind of using pomace, vinasse as the biological feedstuff mixed fungus fermentation method of raw material | |
| CN114107073B (en) | Method for producing hypha protein by utilizing molasses | |
| CN111187731A (en) | Biological bottom-improving algae-culturing water quality improving microbial inoculum and preparation method thereof | |
| CN106495896B (en) | A kind of Pleurotus eryngii waste chaff is culture materials of edible fungi of raw material and preparation method thereof | |
| CN102924141A (en) | Process for utilizing three abandoned proteins and thoroughly-decomposed cow dung to produce growth-promoting bio-organic fertilizer and product thereof | |
| CN101914478A (en) | Bacillus subtilis and application thereof | |
| CN103184174A (en) | Production method of bacillus subtilis biological agent used for sodium humate-containing feed in medium | |
| CN101407762B (en) | Microbial solid inocula, and preparation and use thereof | |
| CN110396480A (en) | A kind of bacillus coagulans XP and its application in Feed Manufacturing | |
| CN103211088A (en) | Preparation method of sea cucumber bait | |
| CN102399699B (en) | Method for producing biological water-purifying agent through microbe mutual fermentation of chicken manure | |
| CN103642695B (en) | One Aspergillus oryzae and the application prepared at fermentable in fodder additives thereof | |
| CN113416673A (en) | Complex microbial inoculant for detoxifying cottonseed meal as well as preparation method and application thereof | |
| CN102898197A (en) | Technology for producing growth promoting bioorganic fertilizer by using algae mud as additive and product | |
| CN115838668B (en) | Preparation method of pig manure composite fermentation microbial inoculum, fermentation microbial inoculum prepared by using same, pig manure bio-organic fertilizer and preparation method and application thereof | |
| CN103749957B (en) | Preparation method for blue-green algae single-cell protein feed | |
| CN111019996A (en) | Method for preparing active polypeptide by liquid fermentation of camellia seed meal | |
| CN107484882A (en) | A kind of method and its application of waste of aquatic harmless treatment | |
| CN103725738A (en) | Method for preparing collagen polypeptides by using larimichthys crocea leftovers | |
| CN1460423A (en) | Production method of straw and stalk microbial fermented feed | |
| CN103820342B (en) | Selenium-enriched yeast and application thereof | |
| CN102334597A (en) | Method for preparing detoxified animal feed from jatropha curcas cake dregs through oyster mushroom fermentation | |
| CN116004399A (en) | Neurospora crassa DZ01 strain and application thereof in fermented eucommia ulmoides leaf feed |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| PE01 | Entry into force of the registration of the contract for pledge of patent right | ||
| PE01 | Entry into force of the registration of the contract for pledge of patent right |
Denomination of invention: Preparation method for blue-green algae single-cell protein feed Effective date of registration: 20181213 Granted publication date: 20150520 Pledgee: Wuxi rural commercial bank Limited by Share Ltd Yixing branch Pledgor: Tianshi Fodder Co., Ltd., Yixing City Registration number: 2018990001202 |
|
| PC01 | Cancellation of the registration of the contract for pledge of patent right | ||
| PC01 | Cancellation of the registration of the contract for pledge of patent right |
Date of cancellation: 20200812 Granted publication date: 20150520 Pledgee: Wuxi rural commercial bank Limited by Share Ltd. Yixing branch Pledgor: SKYSTONE FEED (YIXING) Co.,Ltd. Registration number: 2018990001202 |
|
| PE01 | Entry into force of the registration of the contract for pledge of patent right | ||
| PE01 | Entry into force of the registration of the contract for pledge of patent right |
Denomination of invention: A preparation method of cyanobacteria single cell protein feed Effective date of registration: 20200812 Granted publication date: 20150520 Pledgee: Wuxi rural commercial bank Limited by Share Ltd. Yixing branch Pledgor: SKYSTONE FEED (YIXING) Co.,Ltd. Registration number: Y2020990000944 |