CN103735855A - Traditional Chinese medicine composition containing dendrobium officinale leaves and flowers and application thereof - Google Patents
Traditional Chinese medicine composition containing dendrobium officinale leaves and flowers and application thereof Download PDFInfo
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Abstract
The invention discloses a traditional Chinese medicine composition containing dendrobium officinale leaves and flowers. The composition comprises the following components in parts by weight: 10-25 parts of dendrobium officinale leaves, 1-5 parts of dendrobium officinale flowers, 5-15 parts of fleshfingered citron flowers, 5-20 parts of seville orange flowers, 5-15 parts of feverfew and 5-18 parts of flowers of Japanese apricot. The invention further provides tea prepared by the traditional Chinese medicine composition containing dendrobium officinale leaves and flowers as well as an application of the traditional Chinese medicine composition in preparing medicines for treating functional dyspepsia. The composition disclosed by the invention mainly has the beneficial effects that (1) the composition is reasonable in formula, and has the effects of regulating qi and the middle warmer and replenishing the vital essence and removing heat; (2) the composition is abundant in source, low in cost and beneficial to comprehensive utilization of the dendrobium officinale raw material; (3) through detection, the tea prepared by the composition has the effects of resisting oxidation, eliminating free radicals and the like in vitro; (4) the composition can improve gastrointestinal evacuation to relieve symptoms such as gastrointestinal fullness, and can be used for assistant treatment of diseases such as functional dyspepsia.
Description
(1) technical field
The present invention relates to a kind of Chinese medicine composition containing Herba Dendrobii leaf and flower, and in the application of preparing in functional dyspepsia medicine, and utilize auxiliary treatment prepared by this Chinese medicine composition for tea-drinking.
(2) background technology
Functional dyspepsia (Functional Dyspepsia, be called for short FD) be a clinical comparatively common class functional disease take upper abdominal pain or discomfort, epigastrium burn feeling, glutted, early full sense after the meal etc. as principal character, comprising: 1. 2. two types of upper abdomen syndromes etc. of uncomfortable syndrome after the meal.FD diagnostic criteria includes with the next item down or more than one symptom: feeling of repletion, early full, upper abdominal pain or epigastrium burn feeling after the meal, but to get rid of organic, general, the metabolic disease of soluble this symptom.The disease associations such as FD and chronic gastritis, bile reflux gastritis, gastroesophageal reflux.
The indigestion symptom that has every year 20%-30% to occur for a long time in general population or repeatedly show effect.These data have reflected in crowd without the dyspeptic situation of investigating.Though there is the patient of organic disease comprising heartburn symptom and prompting, only account for dyspepsia patient's fraction, therefore can infer that all the other most patients all exist F D.Some perspective studys show, occurring first in the patient of indigestion symptom, dyspeptic annual morbidity is 1% left and right, and after morbidity, most unknown cause dyspepsia patients have the catabasis, but long-term observation still can Symptomatic outbreak repeatedly.Approximately 50% patient can seek medical advice because of indigestion symptom.Prompting primary disease, clinical comparatively common, is also a large functional disease of a class puzzlement crowd simultaneously.
Chinese medicine thinks that primary disease belongs to stagnation of QI in spleen and stomach and forms, and has obvious dependency with depression of liver-QI simultaneously, and incoordination between the liver and stomach is a large different TCM syndrome types of assertive evidence.Can adopt the treatment rule of depressed liver-energy dispersing and QI regulating to carry out conventional therapy.But, in a large amount of sub-health populations, there is light symptoms crowd's ratio to rise just year by year, this crowd often can initiatively not appeal to Drug therapy, will cause year in year out comparatively serious functional disease, and therapeutic effect is obviously not good.How to intervene in early days, namely occurring that just intervening in early days of comparatively slight functional disease is comparatively effectively means of one.But this class disease of Chinese traditional treatment needs determination for the treatment of based on pathogenesis obtained through differentiation of symptoms and signs ability prescriptions, early stage application just utilizes decoction more for sub-health population, not accept, and is also difficult to meet the requirement of large-scale promotion.Thereby, in the urgent need to developing a kind of Chinese medicinal formulae of determined curative effect.
(3) summary of the invention
The present invention is for a kind of determined curative effect, abundant, the cheap Chinese medicine composition that can be used for functional dyspepsia in medicine source are provided, and the application of this Chinese medicine composition in the various control functional dyspepsia medicines of preparation.
The technical solution used in the present invention is:
Containing a Chinese medicine composition for Herba Dendrobii leaf and flower, the component following by quality proportioning forms (all with dry weight basis):
10～25 parts, Herba Dendrobii leaf
1～5 part of produce dendrobium flowers
5～15 parts of Flos Citri Sarcodactyliss
5～20 parts of CitrusaurantiumL.Var.amara Engl.s
5～15 parts of Flos Chrysanthemis
5～18 parts of flowers bud of Japanese apricot.
In component of the present invention, the quality of each component is with dry weight basis, and each raw material components used is obtainable Chinese medicine dry products on market.Wherein Herba Dendrobii leaf and Hua Jun derive from Zhejiang Province, the Herba Dendrobii of the wild or artificial growth in the area such as Anhui Province, Fujian Province.Herba Dendrobii belongs to a large kind of Herba Dendrobii, especially has establishing in large scale in Zhejiang Province.Wild resource is also extremely abundant.Chinese medicine thinks, Herba Dendrobii is sweet light micro-salty, cold, enters stomach, lung, kidney channel.There is the stomach reinforcing of promoting the production of body fluid, the curative effect of clearing away heat and nourishing YIN, can deal with problems arising from an accident and take good care of for calentura, < < herbal classic > > calls its " tonifying five ZANG-organs asthenia weakness and emaciation; reinforcing YIN-essence; take for a long time thick the intestines and stomach ", is often described as " one of China's nine large Herba mesonae chinensis ".Often with stem, be used as medicine at present, its " leaf ", " flower " Chang great Liang give up when gathering, and do not wait and are maximally utilised.Through content analysis, detect and find, its " leaf " and " flower " also contain higher medicinal price, and effective ingredient is take flavonoid and all kinds of volatilization wet goods as main, are applicable to exploitation and the comprehensive utilization of various generation tea-drinking kind.Herba Dendrobii leaf is the side-product of Herba Dendrobii plantation with flower at present, after being dried, can on market, conveniently obtain by conventional method.
From the formula of this compositions, Herba Dendrobii leaf: contain abundant flavone bioside compounds, there is the pharmacologically active of removing free radical, antioxidation, blood sugar lowering and protection cardiovascular system.Produce dendrobium flowers: contain more multiple volatile ingredient, as aldehyde C-9, Folium eucalypti globueli (Eucalyptus globulus Labill.)-5,11-diene-8,12-lactide, α-cedar wood alcohol etc.From property of traditional Chinese taste, Herba Dendrobii flower has the effect that fragrance is amusing, can activating QI and resolving turbid pathogen, and regulating the function of middle-JIAO QI of the spleen and stomach.Flos Citri Sarcodactylis: be flower and the alabastrum of rutaceae Fructus Citri Sarcodactylis.Function is similar to finger citron sliced, but is used as medicine and more can wanders away with flower.Enter liver, stomach two warps, gas perfume (or spice), mildly bitter flavor, has the merit of suppressing the hyperactive liver peratodynia; Its ethanol extract has obvious inhibitory action to isolated rat intestinal tube.The rabbit duodenum spasm that acetylcholine is caused has significant spasmolysis.CitrusaurantiumL.Var.amara Engl.: having another name called Citrus aurantium L. var. amara Engl., is the alabastrum of rutaceae Citrus aurantium L. var. amara Engl..Containing volatile oil component limonene, linalool, citronellol, valeric acid etc.The sweet micro-hardship of CitrusaurantiumL.Var.amara Engl. property, the merit of have soothing the liver, stomach function regulating, regulating the flow of vital energy, kindly controls that painful abdominal mass is vexed in the heart, abdominal distention, the diseases such as vomiting, few food.Flos Chrysanthemi: claiming again Flos Chrysanthemi, is the head inflorescence of feverfew chrysanthemum.Gas delicate fragrance, lightly seasoned micro-hardship, enters lung, Liver Channel.There is the functions such as dispelling wind, heat clearing away, improving eyesight, removing toxic substances.Contain more volatile oil, flavonoid, aminoacid ingredient and trace element.Many and other Calming liver to disperse Qi decoction compatibilities are used for the treatment of the diseases such as depression of liver-QI.Flower bud of Japanese apricot: claim again FLOS MUME, sour and astringent, flat.Enter liver, lung two warps.The merit of there is soothing the liver, stomach function regulating, reducing phlegm.Be usually used in treating the diseases such as globus hystericus, stomachache due to hyperactive liver-QI attacking the stomach, inappetence.
Functional dyspepsia and gastrointestinal dynamic relation are close, and TCM Syndrome Type is take incoordination between the liver and stomach as main foundation pattern of syndrome.More than in formula, produce dendrobium flowers, leaf are monarch drug, fragrance circulation of qi promoting, have the merit of stomach function regulating yin nourishing concurrently, the product that coordinate flower bud of Japanese apricot, Flos Citri Sarcodactylis, the equal tool depressed liver-energy dispersing and QI regulating of CitrusaurantiumL.Var.amara Engl. and stomach and alleviating pain, Flos Chrysanthemi has the merit of dispelling wind liver heat removing, has the effect of removing toxic substances concurrently simultaneously, coordinate monarch drug to play altogether the merit of dispersing the stagnated liver-QI for regulating the stomach pain relieving, all medicines coordinate the effect that can play treating both the principal and secondary aspects of a disease above, can be used for the treatment of the functional dyspepsia of incoordination between the liver and stomach, are its desirable drug regimens.
The preferred quality proportioning of described Chinese medicine composition is as follows:
The invention still further relates to the application of described Chinese medicine composition in the medicine of preparation treatment functional dyspepsia.
Described medicine can add conventional adjuvant, makes one of following dosage form:
1. decoction; 2. capsule; 3. granule; 4. tablet; 5. oral liquid; 6. for tea-drinking.
The invention still further relates to prepared by a kind of Chinese medicine composition of the present invention for tea-drinking, described component of getting prescription amount for tea-drinking, is mainly used in the auxiliary treatment of functional dyspepsia, chronic gastritis etc., described generation tea-drinking prepare by the following method:
Get the Herba Dendrobii leaf of 10～25 mass parts, the produce dendrobium flowers of 1～5 mass parts, the Flos Citri Sarcodactylis of 5～15 mass parts, the CitrusaurantiumL.Var.amara Engl. of 5～20 mass parts, the Flos Chrysanthemi of 5～15 mass parts, the flower bud of Japanese apricot of 5～18 mass parts, mix latter 60 ℃ and dry to obtain mixture; The mixture of gained is pulverized, and crosses successively 20 orders, 80 mesh sieves, gets the granule of 20 order to 80 order mid portions, weighs and packs filter paper bag into, and every 2 grams of granules are one bag, obtain for tea-drinking tea bag.
The available 150ml boiling water of the every bag of described generation tea-drinking (2g) brews, and 5～10min is drinkable.
The beneficial effect of the Chinese medicine composition containing produce dendrobium flowers and leaf provided by the invention is mainly reflected in: (1) Chinese medicine composition composition formula of the present invention is reasonable, has the effect of depressed liver-energy dispersing and QI regulating and stomach and alleviating pain, with strong points; (2) compositions provided by the invention is with low cost, and crude drug source is abundant, with low cost, is conducive to the comprehensive utilization of Herba Dendrobii raw material; (3) compositions make for tea-drinking after testing, there is in vitro antioxidation, remove the effect such as free radical; (4) can alleviate the spasm of gastrointestinal smooth muscle, promote the emptying of gastrointestinal, the effectively clinical symptoms of the disease such as relieve chronic gastritis, functional dyspepsia, can be for the auxiliary treatment of the diseases such as functional dyspepsia, raising patient's quality of life.
(4) accompanying drawing explanation
The clearance rate comparison diagram to DPPH free radical for tea-drinking of Fig. 1 different formulations.
Fig. 2 different formulations for tea-drinking to ABTS
+the clearance rate comparison diagram of free radical.
Fig. 3 variable concentrations is the influence curve figure to rat small intestine in vitro autonomic movement tension force for tea-drinking.
Fig. 4 variable concentrations is the influence curve figure to rat small intestine in vitro autonomic movement amplitude for tea-drinking.
Fig. 5 variable concentrations is the influence curve figure to rat small intestine in vitro autonomic movement frequency for tea-drinking.
The antagonistic rate comparison diagram of isolated rat small intestinal shrink tension, amplitude and the frequency of Fig. 6 different pharmaceutical to Ach induction.
(5) specific embodiment
Below in conjunction with specific embodiment, the present invention is described further, but protection scope of the present invention is not limited only to this.
Embodiment 1: for tea-drinking formula optimization
One, experimental technique:
1 raw material processing
Get respectively the dry product of Herba Dendrobii leaf, produce dendrobium flowers, Flos Citri Sarcodactylis, CitrusaurantiumL.Var.amara Engl., Flos Chrysanthemi, flower bud of Japanese apricot, 60 ℃ of oven dry; Respectively get 100g dry product and pulverize, cross successively 20 orders, 80 mesh sieves, get 20 order to 80 order mid portion granules, weigh.
The screening of 2 tea bag drink prescriptions
Adopt L
16(4
5) orthogonal table, design 25 group of formula are tested.Orthogonal test factor level table is in Table 1.
Table 1 orthogonal test factor level table
25 group of formula, as table 3 Orthogonal Experiment and Design, are got respectively raw material (20 order to the 80 order mid portion granules that the step 1 makes) consumption of each factor varying level, mix make 25 kinds of formulas for tea-drinking.
3 sensory reviews
Get the 2g sample (specification 2g/ bag) of respectively filling a prescription, add after 150mL boiling water bubble 5min, it is carried out to sensory evaluation.With reference to GB/T24690-2009, formulate following organoleptic quality standards of grading, best result: flavour is that 30 points, fragrance are 20 points, and soup color is 10 points, evaluates standard as shown in table 2.Each processing score value is carried out to statistical analysis, determine profile error ratio.
Table 2 sensory review reference standard
According to results of sensory evaluation, can tea bag drink be divided into excellent, good, in, differ from four grades.
Excellent: flavour is pure, there is good fragrance of a flower abnormal smells from the patient, without assorted different odor, soup color is bright without precipitation, brews rear filter paper bag and rises and do not break.
Good: though flavour is pure, there is good fragrance of a flower abnormal smells from the patient, few fresh taste, soup color is bright but fresh and alive not.Brew rear filter paper bag flawless.
In: flavour is purer, and fragrance is gentle, and soup color depth is dark, sometimes has a small amount of tea grounds and spill after brewing.
Poor: flavour, fragrance are undesired, and soup color contamination is turbid, has different odor, brews rear loose bag.
4 determination of bioactive constituent
4.1 determination of total flavonoids
4.1.1 the drafting of rutin standard curve
Accurately draw concentration and be 150 μ g/mL rutin standard solution 0,0.50,1.00,2.00,3.00,4.00mL, move in 10mL scale color comparison tube, add distilled water to 5mL, respectively add 5% sodium nitrite solution 0.3mL, after jolting, place 5min, add 10% aluminum nitrate solution 0.3mL, shake up rear placement 6min, add 1.0mol/L NaOH solution 2mL, be settled to scale with distilled water, take zero pipe as blank, shake up the rear 1cm of using cuvette, at 510nm place, measure light absorption value, take optical density as vertical coordinate, the content of flavone is abscissa, drawing standard curve.
4.1.2 the compound flower tea bag mensuration of general flavone content in sample of making tea
Get 1mL teabag soak, by the method for standard curve, measure light absorption value, and convert and obtain general flavone content in teabag soak according to standard curve,, further convert and obtain the general flavone content in 2g teabag dry powder sample.
4.2 total phenol contents are measured
4.2.1 the drafting of gallic acid standard curve
Accurately draw concentration and be the gallic acid standard solution 0,0.1,0.2,0.4,0.6,0.8mL of 100 μ g/mL in 10mL color comparison tube, add the Na of 5mL forint phenol reagent, 4mL75g/L
2cO
3solution.Vortex mixes after 15s, is settled to scale with distilled water, places 30min for 40 ℃, colour developing.With the light absorption value of spectrophotometric determination 765nm, take optical density as vertical coordinate, the content of total phenols is abscissa, drawing standard curve.
4.2.1 the compound flower tea bag mensuration of total phenol content in sample of making tea
Get 1mL teabag soak, by the method for standard curve, measure light absorption value, and convert and obtain total phenol content in teabag soak according to standard curve, further convert and obtain the total phenol content in 2g teabag dry powder sample.
4.3 radical scavenging activities are measured
4.3.1 scavenging ability of DPPH free radical is measured
Get 2 × 10 of 1mL sample liquid and 1mL
-4mol/L DPPH solution shakes up, and under room temperature, lucifuge is measured its absorbance A after placing 30min under 517nm
i, measure 1mL DPPH solution and the absorbance A c of 1mL dehydrated alcohol mixed liquor under 517nm simultaneously, and 1mL sample liquid and the absorbance A of 1mL dehydrated alcohol mixed liquor under 517nm
j.
DPPH free radical scavenging activity (%)=[1-(A
i-A
j)/Ac] × 100
4.3.2 remove ABTS
+free radical ability is measured
Get the sample solution 0.4mL of variable concentrations, add the 0.0875mM ABTS of 3.6mL
+solution, after whirlpool mixes in dark place standing 10min, measure the light absorption value of 734nm.Control tube substitutes sample liquid with isopyknic 70% ethanol.
Two, experimental result
1 sensory review's result
From table 3 orthogonal experiments, according to the size of extreme difference R, B > E > D > C > F > A, illustrate that Flos Citri Sarcodactylis is teabag organoleptic indicator's major influence factors, other factors are followed successively by flower bud of Japanese apricot, Flos Chrysanthemi, CitrusaurantiumL.Var.amara Engl., Herba Dendrobii leaf and Herba Dendrobii flower.By intuitive analysis, be combined as preferably as seen A
3b
3c
5d
5e
2f
2, i.e. 0.9g Herba Dendrobii flower, 12g Flos Citri Sarcodactylis, 15g CitrusaurantiumL.Var.amara Engl., 7.5g Flos Chrysanthemi, 10g flower bud of Japanese apricot and 16g Herba Dendrobii leaf, but this formula combination does not appear in orthogonal test table.For the further impact of this result of checking on formula for a product, with A
3b
3c
5d
5e
2f
2for condition is carried out formula test, result sensory review's total score is 57.
Table 3 orthogonal experiments
2 total flavones and determining total phenol result
Two the formula As the highest to sensory review's score
3b
1c
3d
5e
2f
4(formula 1), A
4b
3c
1d
4e
2f
5(formula 2) and optimal case A
3b
3c
5d
5e
2f
2in (formula 3) teabag, total flavones, total phenol content are measured, and result is as shown in table 4.
Table 4 different formulations is for total flavones and total phenol content measurement result in tea-drinking dry powder sample
3 antioxidation in vitro result of the tests
3.1 scavenging ability of DPPH free radical
Get three formula (A
3b
1c
3d
5e
2f
4(formula 1), A
4b
3c
1d
4e
2f
5(formula 2), A
3b
3c
5d
5e
2f
2(formula 3)) teabag contrast with Vc, teabag is soaked in the boiling water of different volumes amount, the quality of teabag and the volume ratio of water are sample liquid concentration, according to step 4.3.1, measure teabag sample liquid and the Vc sample liquid scavenging ability of DPPH free radical of variable concentrations, record the absorbance of 517nm, calculate DPPH free radical scavenging activity, take sample liquid, add after reagent the final concentration after dilution as abscissa, DPPH free radical scavenging activity is vertical coordinate, and curve plotting as shown in Figure 1.
As shown in Figure 1, the medical flower compound bag-soak-tea of three formulas can effectively suppress the generation of DPPH free radical, and has within the specific limits dose-effect relationship, its IC
50be respectively 13.31 μ g/mL, 12.75 μ g/mL and 13.04 μ g/mL, positive control V
ciC
50=4.42 μ g/mL.
3.2 remove ABTS
+free radical ability
Get three formula (A
3b
1c
3d
5e
2f
4(formula 1), A
4b
3c
1d
4e
2f
5(formula 2), A
3b
3c
5d
5e
2f
2(formula 3)) teabag and Vc contrast, teabag is soaked in the boiling water of different volumes amount, the quality of teabag and the volume ratio of water are sample liquid concentration, according to the teabag sample liquid of step 4.3.2 mensuration variable concentrations and Vc sample liquid removing ABTS
+free radical ability, records the absorbance data of 734nm, calculates ABTS
+free radical scavenging activity, adds the concentration at the end after dilution after reagent as abscissa take sample liquid, ABTS
+free radical scavenging activity is vertical coordinate, and curve plotting as shown in Figure 2.
As shown in Figure 2, the teabag of three formulas all can effectively suppress ABTS
+the generation of free radical, and there is within the specific limits dose-effect relationship, its IC
50be respectively 22.30 μ g/mL, 10.69 μ g/mL and 13.79 μ g/mL, with positive control V
c(IC
50=17.01 μ g/mL) quite.
4 experimental results: carry out orthogonal design with combination of Chinese medicine material preferred, A
3b
3c
5d
5e
2f
2scheme is to carry out from now on the preferred version that medicine group is complete, and this formula has had the feature of mouthfeel, stable content concurrently, and has significant effect removing aspect free radical, and contemporary tea-drinking form is easy to use, can be for the auxiliary treatment of relevant disease.
Embodiment 2: the research for tea-drinking to isolated rat bowel movement effect
1. research purpose
Utilize in vitro intestinal smooth muscle bar power determination method, observation is the impact on isolated rat intestinal tension force, amplitude and frequency for tea-drinking, and compare with XIANGSHA LIUJU WAN and normal saline, observe the impact of tension force, amplitude and the frequency on the intestinal smooth muscle bar under acetylcholine-induced for tea-drinking decoct.
2. research method
Get SD isolated rat small intestinal, by in vitro intestinal smooth muscle perfusion experimental technique, observe and add various dose shrink situation and under acetylcholine effect, add respectively the contraction situation for isolated rat intestinal smooth muscle after tea-drinking, XIANGSHA LIUJU WAN and normal saline for the rear isolated rat intestinal smooth muscle of tea-drinking (in bath, final concentration is 0.0185g/ml, 0.0370g/ml, 0.0740g/ml, 0.1480g/ml).
3. experiment material and method
3.1. laboratory animal healthy SD rat, clean level, male, body weight 200-300g, six week age, Shanghai western pul-Bi Kai laboratory animal company limited, quality certification numbering: SCXK (Shanghai) 2008-0016, is provided by Zhejiang University of Traditional Chinese Medicine's Animal Experimental Study center.
3.2. experimental drug and reagent
For tea-drinking, get in embodiment 1 for tea-drinking (A
3b
3c
5d
5e
2f
2formula) 24g (12 bags), heating water 480ml soaks 5min,, decocting 3min, evaporation and concentration is to every milliliter contained amount 1.0g, after decompress filter, standby; The material 6g (3 bags) that gets it filled, is mixed with every milliliter contained amount 0.25g with method, standby;
Acecoline: Aladdin industrial group, product batch number: A111014;
XIANGSHA LIUJU WAN: Beijing TongrenTang Pharmaceutical Co., Ltd, product batch number: 3082071; Take 25g, add water 250ml and decoct 5min, decompress filter, filtrate evaporation and concentration to concentration is 1.0g/ml, after refiltering, standby; Take 6g, with method, be mixed with its concentration and be 0.25g/ml, standby;
Tyrode's solution: be made into and contain NaCl8.0g with distilled water, KCl0.2g, MgSO
47H
2o0.26g, NaH
2pO
42H
2o0.065g, NaHCO
31.0g, CaCl
20.2g, the 1000ml solution of glucose 1.0g, standby; Prepare tyrode's solution NaCl used, KCl, MgSO
47H
2o, NaH
2pO
42H
2o, NaHCO
3, CaCl
2, glucose is analytical pure.
4. experimental technique
4.1 experimental techniques are got one of SD rat, fasting 24h before experiment, freely drink water, de-neck is cut abdominal cavity open after putting to death immediately, find small intestinal, take out, separate mesentery and its content rinsed well with tyrode's solution, be cut into respectively 2-3cm intestinal tube, be stored in low temperature (4-6 ℃) containing gaseous mixture (95%O
2+ 5%CO
2) in saturated tyrode's solution, standby.
By in vitro intestinal smooth muscle, pour into experimental technique with automatic oxygen supply sustainable supply gaseous mixture (95%O
2+ 5%CO
2), intestinal tube one end is fixed on ventilation hook, the other end is connected and fixed on tonotransducer and causes computer interface.Water-bath keeps temperature (37 ± 0.5) ℃ in bath, passes into gaseous mixture 60-80 bubble/min, specimen load 1g, and chart speed 4mm/min, range 20mv, balance 30-45min, between specimen stable phase, every 15min renews fresh tyrode's solution once.Record one section of normal contraction curve, start to add tested medicine.Medicine adds in temperature chamber with microsyringe.After every experiment, rinse specimen with tyrode's solution, every minor tick 10min, totally 3 times, shrinks after stable and just carries out next step experiment until intestinal segment.
4.2 experiment content
4.2.1 the impact on isolated rat intestinal autonomic movement for tea-drinking
Specimen is stablized 1h, produce after regular constant self-discipline, first record contrast state smoothing flesh activity curve 5min, and then with bath in add various dose for tea-drinking, making its final concentration is (0.0185g/ml, 0.0370g/ml, 0.0740g/ml, 0.1480g/ml), 21min is observed in each dosing, records amplitude, mean tension and frequency change that medicine is had an effect.
The impact of 4.2.2 for tea-drinking decoct, the isolated rat intestinal of Ach induction being shunk
Specimen is stablized 1h, produces after the self-disciplining that rule is constant, and first record contrast state smoothing flesh activity curve 5min then adds acetylcholine Ach (final concentration 10 in bath
-5mol/L), when reaching maximum collapse, adding for tea-drinking makes its final concentration for (0.0740g/ml), each observation post administration 5min that gives, record amplitude, mean tension and frequency change that medicine is had an effect, with the amplitude before administration, mean tension and frequency, be worth in contrast, amplitude, mean tension and frequency action effect value after administration, represent percent change=(effect value-control value)/control value × 100% by percent change.The operating procedure of XIANGSHA LIUJU WAN (0.0740g/ml) positive controls and normal saline negative control group is the same.
4.3 statistical method
Every data are all with mean ± standard deviation
represent, each group of data are carried out to statistical analysis with SPSS software statistics bag.Two groups of means are relatively with t check, and between many groups, mean is relatively used variance analysis, between mean, relatively with q, checks between two.
5. experimental result
5.1 impacts of generation tea-drinking on isolated rat intestinal autonomic movement
The impact (n=6) of table 5 generation tea-drinking decoct on rat small intestine in vitro autonomic movement tension force (g)
With before administration relatively: * P<0.01, * * P<0.05; Compare with 0.0185g/ml group:
##p<0.05; Compare with 0.0370g/ml group:
△ △p<0.05.
Result shows, for tea-drinking decoct, rat small intestine autonomic movement is to obvious inhibitory action, shows as amplitude and weakens, and tension force declines, frequency deceleration, and strengthen with dosage, effect is strengthened, and extend action time.
Table 5 shows, compared with before administration, for the tension force of tea-drinking decoct all the other each dosage groups except 0.0185g/ml group, all obviously reduces (P<0.01 or P<0.05); After administration, concentration is the 0.0185g/ml group tension force time of reducing only to continue 3 minutes (P<0.05), have afterwards by a small margin and rise, 0.0370g/ml group tension force reduces time remaining 15 minutes (P<0.05), and all the other dosage group tension force reduce time remaining 21 minutes (P<0.01 or P<0.05); Between each dosage group, be obvious dose-effect relationship (P<0.05); After administration, each dosage group temporal evolution tension force is without significant change (P>0.05).Tension variation curve chart is shown in Fig. 3.
Table 6 shows, compared with before administration, is the amplitude reduction (P<0.01 or P<0.05) of 0.0740g/ml group and 0.1480g/ml group for tea-drinking decoct concentration; After administration, its amplitude time of reducing continues respectively 15 minutes (P<0.01 or P<0.05) and continues 21 minutes (P<0.01 or P<0.05); 0.0740g/ml and 0.1480g/ml group obviously reduce (P<0.01 or P<0.05) compared with 0.0185g/ml and 0.0370g/ml group amplitude; After administration each dosage group over time amplitude without significant change (P>0.05).Amplitude variations curve chart is shown in Fig. 4.
The impact (n=6) of table 6 generation tea-drinking on rat small intestine in vitro autonomic movement amplitude (cm)
With before administration relatively: * P<0.01, * * P<0.05; Compare with 0.0185g/ml group:
##p<0.05; Compare with 0.0370g/ml group:
△ △p<0.05.
Table 7 shows: compared with before administration, for the frequency of the each dosage group of tea-drinking decoct, all reduce (P<0.01 or P<0.05); After administration, 0.0185g/ml class frequency reduces time remaining 9 minutes (P<0.05), all the other dosage class frequencys reduce time remaining 21 minutes (P<0.01 or P<0.05), and each dosage group is obvious dose-effect relationship (P<0.01 or P<0.05); After administration each dosage group group over time frequency without significant change (P>0.05).Frequency variation curve figure is shown in Fig. 5.
Table 7 generation tea-drinking on rat small intestine in vitro autonomic movement frequency (beat/min) impact (n=6)
The tea-drinking decoct impact that the isolated rat intestinal of Ach induction is shunk of 5.2 generations
Result shows, Ach (10
-5mol/L) cause the strong contraction of isolated rat small intestinal, with comparison before administration, its tension force obviously increases to 1.77 ± 0.62 (P<0.01), frequency is increased to 31.67 ± 4.06 (P<0.05), and amplitude is without significant change (P>0.05).With the comparison of normal saline negative control group, the increase (P<0.01) of increasing (P<0.01) and frequency for tea-drinking decoct (0.0740g/ml) tension force that obviously antagonism acetylcholine causes, but on amplitude without impact (P>0.05), XIANGSHA LIUJU WAN (0.0740g/ml) the positive controls tension force that obviously antagonism acetylcholine causes increases (P<0.01), but on amplitude and frequency without impact (P>0.05); With the comparison of XIANGSHA LIUJU WAN (0.0740g/ml) positive controls, for tea-drinking decoct (0.0740g/ml), frequency is had to significant difference (P<0.01), on tension force and amplitude without impact (P>0.05).Concrete antagonistic rate contrast situation as shown in Figure 6.
6. analyze and discuss
By above result, shown, the tension force for tea-drinking decoct to isolated rat bowel movement, amplitude, frequency all have obvious inhibitory action, and present certain dose-effect relationship.
Acetylcholine (Ach) is a kind of neurotransmitter of Gastrointestinal motility adjustment, can cause that pipe smooth muscle shrinks.Its mechanism of action is: the M-receptors bind on Ach and smooth muscle surface, open current potential sensitivity Ca
2+passage or receptor activation Ca
2+passage, causes extracellular Ca
2+interior stream, Ca in cell
2+concentration raises, sarolemma depolarization.Ca in born of the same parents
2+concentration raises, and Secondary cases causes cyclic guanosine monophosphate (cGMP) increased content in born of the same parents, thereby promotes endoplasmic reticulum to discharge Ca
2+, thereby Ca in cell
2+concentration further increases, and causes smooth muscle contraction.By above experiment, found: the intestinal contraction movement causing for tea-drinking decoct energy antagonism acetylcholine, its antagonism to tension force and frequency is remarkable, and it is remarkable compared with XIANGSHA LIUJU WAN effect to the antagonism of frequency.
Enterospasm is the pathogenesis basis of many digestive tract disease, and some spasmolytic medicines are as large in side effect such as atropine, to the research acting in gastrointestinal movement for tea-drinking decoct, will be the anti-enterospasm drug provision of development of new theoretical foundation.
In sum: for tea-drinking decoct, can reduce smooth muscle tension force, remove spasm, to recovering gastrointestinal smooth myokinesis and an equilibrium of forces, adjust gastrointestinal motility disorder undoubtedly, be very useful.
Claims (6)
1. containing a Chinese medicine composition for Herba Dendrobii leaf and flower, the component following by quality proportioning forms:
10～25 parts, Herba Dendrobii leaf
1～5 part of produce dendrobium flowers
5～15 parts of Flos Citri Sarcodactyliss
5～20 parts of CitrusaurantiumL.Var.amara Engl.s
5～15 parts of Flos Chrysanthemis
5～18 parts of flowers bud of Japanese apricot.
2. Chinese medicine composition as claimed in claim 1, is characterized in that described Chinese medicine composition amount proportioning is as follows:
3. the application of Chinese medicine composition as claimed in claim 1 or 2 in the medicine of preparation treatment functional dyspepsia.
4. application as claimed in claim 3, is characterized in that described medicine makes one of following dosage form: (1) decoction; (2) capsule; (3) granule; (4) powder; (5) oral liquid; (6) for tea-drinking.
With claimed in claim 1 containing the Chinese medicine composition of Herba Dendrobii leaf and flower, prepare for a tea-drinking, described component of getting prescription amount for tea-drinking, prepares by the following method:
Get the produce dendrobium flowers of 1～5 mass parts, the Flos Citri Sarcodactylis of 5～15 mass parts, the CitrusaurantiumL.Var.amara Engl. of 5～20 mass parts, the Flos Chrysanthemi of 5～15 mass parts, the flower bud of Japanese apricot of 5～18 mass parts, the Herba Dendrobii leaf of 10～25 mass parts, mix latter 60 ℃ and dry to obtain mixture; The mixture of gained is pulverized, and crosses successively 20 orders, 80 mesh sieves, gets the granule of 20 order to 80 order mid portions, weighs and packs filter paper bag into, and every 2 grams of granules are one bag, obtain for tea-drinking tea bag.
6. the application of generation as claimed in claim 5 tea-drinking in the medicine of preparation treatment functional dyspepsia.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104666930A (en) * | 2015-01-29 | 2015-06-03 | 百色红色福地农业科技开发有限公司 | Preparation method of dendrobium candidum flower capsule |
| CN109170050A (en) * | 2018-09-25 | 2019-01-11 | 休宁县春之斛生物科技有限公司 | A kind of dendrobium nobile scented tea for health care and preparation method thereof |
| CN111000943A (en) * | 2019-12-31 | 2020-04-14 | 高加喜 | Antidiarrheal composition for treating intestinal digestive diseases and preparation method thereof |
| CN114796073A (en) * | 2022-05-05 | 2022-07-29 | 浙江工业大学 | Anti-aging and anti-wrinkle dendrobium officinale essence and preparation method and application thereof |
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2013
- 2013-12-17 CN CN201310693517.9A patent/CN103735855A/en active Pending
Non-Patent Citations (2)
| Title |
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| 吕圭源等: "铁皮石斛功效相关药理作用研究进展", 《中国中药杂志》 * |
| 李光等: "试析花类药性能特点", 《江西中医药》 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104666930A (en) * | 2015-01-29 | 2015-06-03 | 百色红色福地农业科技开发有限公司 | Preparation method of dendrobium candidum flower capsule |
| CN109170050A (en) * | 2018-09-25 | 2019-01-11 | 休宁县春之斛生物科技有限公司 | A kind of dendrobium nobile scented tea for health care and preparation method thereof |
| CN111000943A (en) * | 2019-12-31 | 2020-04-14 | 高加喜 | Antidiarrheal composition for treating intestinal digestive diseases and preparation method thereof |
| CN114796073A (en) * | 2022-05-05 | 2022-07-29 | 浙江工业大学 | Anti-aging and anti-wrinkle dendrobium officinale essence and preparation method and application thereof |
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Application publication date: 20140423 |