CN103733881B - A kind of manufacture method of Antrodia camphorata original seed - Google Patents
A kind of manufacture method of Antrodia camphorata original seed Download PDFInfo
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- CN103733881B CN103733881B CN201310719336.9A CN201310719336A CN103733881B CN 103733881 B CN103733881 B CN 103733881B CN 201310719336 A CN201310719336 A CN 201310719336A CN 103733881 B CN103733881 B CN 103733881B
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- cinnamomum
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- 241001486992 Taiwanofungus camphoratus Species 0.000 title claims abstract description 55
- 238000000034 method Methods 0.000 title claims abstract description 11
- 238000004519 manufacturing process Methods 0.000 title claims abstract description 9
- 239000001963 growth medium Substances 0.000 claims abstract description 54
- 238000011218 seed culture Methods 0.000 claims abstract description 33
- 235000013312 flour Nutrition 0.000 claims abstract description 32
- 239000002023 wood Substances 0.000 claims abstract description 32
- 241000723347 Cinnamomum Species 0.000 claims abstract description 24
- DSSYKIVIOFKYAU-XCBNKYQSSA-N (R)-camphor Chemical compound C1C[C@@]2(C)C(=O)C[C@@H]1C2(C)C DSSYKIVIOFKYAU-XCBNKYQSSA-N 0.000 claims abstract description 8
- 241000723346 Cinnamomum camphora Species 0.000 claims abstract description 7
- 241000386927 Cinnamomum micranthum f. kanehirae Species 0.000 claims abstract description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 16
- OSGAYBCDTDRGGQ-UHFFFAOYSA-L calcium sulfate Chemical compound [Ca+2].[O-]S([O-])(=O)=O OSGAYBCDTDRGGQ-UHFFFAOYSA-L 0.000 claims description 10
- 239000000203 mixture Substances 0.000 claims description 10
- 235000015099 wheat brans Nutrition 0.000 claims description 10
- 229930006000 Sucrose Natural products 0.000 claims description 8
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 8
- 239000000706 filtrate Substances 0.000 claims description 8
- 239000005720 sucrose Substances 0.000 claims description 8
- 229920001817 Agar Polymers 0.000 claims description 6
- 229920000742 Cotton Polymers 0.000 claims description 6
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 6
- 239000008272 agar Substances 0.000 claims description 6
- 239000008103 glucose Substances 0.000 claims description 6
- 238000002386 leaching Methods 0.000 claims description 2
- 239000000463 material Substances 0.000 abstract description 8
- 241000283690 Bos taurus Species 0.000 abstract description 3
- 229960000846 camphor Drugs 0.000 abstract description 2
- 229930008380 camphor Natural products 0.000 abstract description 2
- 239000000758 substrate Substances 0.000 abstract description 2
- 241000196324 Embryophyta Species 0.000 description 17
- 241000894006 Bacteria Species 0.000 description 15
- 238000012360 testing method Methods 0.000 description 14
- 230000001954 sterilising effect Effects 0.000 description 8
- 241000222336 Ganoderma Species 0.000 description 6
- 239000004743 Polypropylene Substances 0.000 description 5
- -1 polypropylene Polymers 0.000 description 5
- 229920001155 polypropylene Polymers 0.000 description 5
- 241000233866 Fungi Species 0.000 description 4
- 239000011521 glass Substances 0.000 description 4
- 238000002360 preparation method Methods 0.000 description 4
- 239000002994 raw material Substances 0.000 description 4
- 238000012549 training Methods 0.000 description 4
- 241000123370 Antrodia Species 0.000 description 3
- 238000011160 research Methods 0.000 description 3
- 241000222382 Agaricomycotina Species 0.000 description 2
- 241000222341 Polyporaceae Species 0.000 description 2
- 241000222383 Polyporales Species 0.000 description 2
- 240000000111 Saccharum officinarum Species 0.000 description 2
- 235000007201 Saccharum officinarum Nutrition 0.000 description 2
- 230000004913 activation Effects 0.000 description 2
- 238000012258 culturing Methods 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 238000011081 inoculation Methods 0.000 description 2
- 238000012546 transfer Methods 0.000 description 2
- 241000221198 Basidiomycota Species 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 229940025250 camphora Drugs 0.000 description 1
- 239000010238 camphora Substances 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000000855 fermentation Methods 0.000 description 1
- 230000004151 fermentation Effects 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 239000011159 matrix material Substances 0.000 description 1
- 230000003071 parasitic effect Effects 0.000 description 1
- 230000000144 pharmacologic effect Effects 0.000 description 1
- 239000010979 ruby Substances 0.000 description 1
- 229910001750 ruby Inorganic materials 0.000 description 1
Abstract
The invention discloses the manufacture method of a kind of Antrodia camphorata original seed, it is characterised in that comprise the following steps: be inoculated into by Antrodia camphorata in activated inclined plane culture medium and carry out cellar culture, then inoculate and on pedigree seed culture medium, carry out cellar culture, obtain Antrodia camphorata original seed；Described activated inclined plane culture medium is to replace the Cinnamomum kanahirai hay trees bits in the routine activated inclined plane culture medium containing Cinnamomum kanahirai hay tree (Cinnamomum kanehirai) wood flour with Lignum cinnamomi camphorae (Cinnamomum camphora (L.) Presl.) wood flour, and described pedigree seed culture medium is to replace the Cinnamomum kanahirai hay trees bits in the routine pedigree seed culture medium containing Cinnamomum kanahirai hay trees bits with Lignum cinnamomi camphorae wood flour.The present invention uses common Lignum cinnamomi camphorae (Cinnamomum camphora (L.) Presl.) to replace Cinnamomum kanahirai hay tree; make Antrodia camphorata pedigree seed culture medium; new substrate explored by artificial culture for Antrodia camphorata; to replace rare Cinnamomum kanahirai hay trees material, the artificial culture of Antrodia camphorata and the protection of cattle Camphor resources are significant.
Description
Technical field:
The invention belongs to rare medicinal fungus domestication culture techniques field, be specifically related to the manufacture method of a kind of Antrodia camphorata original seed.
Background technology:
Antrodia camphorata also known as Antrodia Camphorata or Antrodia camphorata, belongs to Basidiomycota (Basidomycota), Basidiomycotina (Basidiomycotina),
Hymenomycetes (Hymenomycetes), Aphyllophorales (Aphyllophorales), Polyporaceae (Polyporaceae), Antrodia belongs to
(Antrodia), formal name used at school is [Antrodia camphorata (M.Zang&C.H.Su) Sheng H.Wu, Ryvarden&T.T.
Chang], its another common Latin entitled [Antrodia cinnamomea T.T.Chang&W.N.Chou], actually jljl is different
Name.Initially, solemn by continent scholar a surname and Taiwan's scholars Su Qing China reports in nineteen ninety, jointly for Ganoderma novel species Antrodia camphorata
(Ganoderma comphoratum), is to have been delivered as Ganoderma because type specimen has been infected with Ganoderma spore by mistake
(Ganoderma) member.Reconfigure by Taiwan's scholars Wu's good reputation and Zhang Dongzhu etc. and be born in the year of cattle Antrodia camphorata for present Antrodia.
Antrodia camphorata is the peculiar fungus in Taiwan, is only grow on Taiwan distinctive Cinnamomum kanahirai hay tree (Cinnamomum kanehirai).Cinnamomum kanahirai hay
Tree (Cinnamomum kanehirai Hay) is the island of Taiwan distinctive child care class seeds, belongs to evergreen broad-leaved megaphanerophyte, with the most normal
The Lignum cinnamomi camphorae seen is different, and leaf is about 15cm, width about 9cm, and annual June to October is trophophase, is grown on height above sea level 200～2000m
Mountain area.Up to the present, Antrodia camphorata is the upper unique domestomycetes found of Cinnamomum kanahirai hay tree, and its parasitics is not strong, and Cinnamomum kanahirai hay tree is the most therefore
And dead, centuries of can surviving.
Owing to Antrodia camphorata is considered the unique and medicinal fungi of preciousness in Taiwan, therefore there is high research and commercial value, be also
The wild fungus that Taiwan is the most expensive at present, is referred to as " Ganoderma " in Hong Kong, Macao and Taiwan, and Taiwan is among the people referred to as " ruby in forest ".
And wild Antrodia camphorata is the rarest, the artificial culture of Antrodia camphorata sporophore then needs to fell Cinnamomum kanahirai hay tree as culture matrix, therefore in order to protect
Cattle Camphor resources, need to improve exploration to existing Antrodia camphorata artificial cultivation technique.
About the research of Antrodia camphorata, the nearly more than ten years successively have tens place scholar to carry out the research of various aspects, including form in Taiwan
, molecular biology, liquid fermentation, component analysis and pharmacological action etc., but the domesticating and cultivating of Antrodia camphorata is due to by planting material
Limitation, is a difficult point so far.
Summary of the invention:
It is an object of the invention to provide the manufacture method of a kind of Antrodia camphorata original seed, this manufacture method is to utilize common Lignum cinnamomi camphorae (Cinnamom
Um camphora (L.) Presl.) replace rare Cinnamomum kanahirai hay trees material, it is achieved that the artificial culture of Antrodia Camphorata.
The manufacture method of the Antrodia camphorata original seed of the present invention, it is characterised in that comprise the following steps: Antrodia camphorata is inoculated into activated inclined plane training
Support and carry out cellar culture on base, then inoculate and on pedigree seed culture medium, carry out cellar culture, obtain Antrodia camphorata original seed；
Described activated inclined plane culture medium is to replace routine to contain with Lignum cinnamomi camphorae (Cinnamomum camphora (L.) Presl.) wood flour
Cinnamomum kanahirai hay trees bits in the activated inclined plane culture medium of Cinnamomum kanahirai hay tree (Cinnamomum kanehirai) wood flour, described Primary spawn
Base is to replace the Cinnamomum kanahirai hay trees bits in the routine pedigree seed culture medium containing Cinnamomum kanahirai hay trees bits with Lignum cinnamomi camphorae wood flour.
Preferably, described activated inclined plane culture medium every liter is so prepared: by boil water to 200g Rhizoma Solani tuber osi and 100g Lignum cinnamomi camphorae wood flour,
Cross leaching filtrate, in filtrate, then add 20g glucose and 20g agar, be settled to 1L with water.Sterilizing is standby.
Preferably, described pedigree seed culture medium, based on total mass fraction 100%, by 44～78% Lignum cinnamomi camphorae wood flour, 0～44% cotton seed hulls,
10～20% wheat bran, 1% sucrose and 1% Gypsum Fibrosum composition.Sterilizing is standby.
Another object of the present invention is to provide Lignum cinnamomi camphorae wood flour and replaces the application in cultivating Antrodia camphorata of the Cinnamomum kanahirai hay trees bits.
The present invention uses common Lignum cinnamomi camphorae (Cinnamomum camphora (L.) Presl.) to replace Cinnamomum kanahirai hay tree, makes the training of Antrodia camphorata original seed
Supporting base, the artificial culture for Antrodia camphorata explores new substrate, to replace rare Cinnamomum kanahirai hay trees material, artificial culture and the Cinnamomum kanahirai hay to Antrodia camphorata
The protection of tree resource is significant.
Accompanying drawing illustrates:
Fig. 1 is that the Antrodia camphorata speed of growth on two kinds of different slant mediums compares；
Fig. 2 is that the Antrodia camphorata speed of growth in two kinds of different base material pedigree seed culture mediums compares；
Fig. 3 is that the Antrodia camphorata speed of growth in two kinds of different base material pedigree seed culture mediums compares.
Detailed description of the invention:
Following example are to further illustrate the present invention rather than limitation of the present invention.
Embodiment 1:
Experimental strain: Antrodia camphorata
Experimental procedure:
1, the preparation of activated inclined plane culture medium:
The raw material of every liter of culture medium: Rhizoma Solani tuber osi (peeling) 200 grams, glucose 20 grams, 20 grams of agar, 100g Lignum cinnamomi camphorae wood flour.
By (20～30min) to boil water to Rhizoma Solani tuber osi and Lignum cinnamomi camphorae wood flour, filtered through gauze, obtain filtrate, filtrate adds glucose and agar, so
1L is complemented to afterwards with water, 121 DEG C of sterilizing 30min, it is subsequently poured in test tube, is configured to the test tube slant training of 18mm × 180mm
Support base.
2, Antrodia camphorata mother plant tube activation: aseptically inoculate, cut from Antrodia camphorata original strain one piece of about 5mm × 5mm,
The strain block of thick about 2mm, proceeds in the activated inclined plane culture medium that step 1 prepares；Using conventional PDA culture medium as comparison.
Two kinds of culture medium do 5 repetitions respectively.
3, bacteria record: be placed in quiescent culture 8d in 28 ± 1 DEG C of lucifuge incubators, treats that mycelium front end forms smooth consistent bacterium
During silk edge line, the most recordable line is as mycelial growth rate start line.Then record a mycelia edge line every 24h,
The numerical value of record 8d is as statistical data continuously.
4, the preparation of pedigree seed culture medium:
(1) Lignum cinnamomi camphorae wood flour pedigree seed culture medium: based on total mass fraction 100%, by 78% Lignum cinnamomi camphorae wood flour, 20% wheat bran, 1% sugarcane
Sugar and 1% Gypsum Fibrosum composition.According to the above ratio, after Lignum cinnamomi camphorae wood flour, wheat bran, sucrose and Gypsum Fibrosum are mixed, add water moistening and mix thoroughly,
Water content 55-60%, uses after fermenting one day.
(2) weed tree sawdust pedigree seed culture medium: based on total mass fraction 100%, by 78% common weed tree sawdust, 20% wheat bran, 1% sugarcane
Sugar and 1% Gypsum Fibrosum composition.According to the above ratio, after common weed tree sawdust, wheat bran, sucrose and Gypsum Fibrosum are mixed, add water moistening and mix thoroughly,
Water content 55-60%, uses after fermenting one day.
5, raw material subpackage sterilizing: Lignum cinnamomi camphorae wood flour pedigree seed culture medium and weed tree sawdust pedigree seed culture medium are distributed into 30mm × 250mm respectively
Teat glass and the polypropylene bacterium bag of 13cm × 27cm, install rear 121 DEG C of sterilizing 1h, material temperature be down to less than 25 DEG C standby.
6, inoculation: by the Antrodia camphorata strain transfer through step 2 activated inclined plane culture medium culturing to the glass examination processed through step 5
Pipe is with in the Lignum cinnamomi camphorae wood flour in polypropylene bacterium bag and weed tree sawdust pedigree seed culture medium, and every 18mm × 180mm test tube slant can connect
The test tube original seed 6-8 of 30mm × 250mm props up or the polypropylene bacterium bag original seed 3-5 bag of 13cm × 27cm.With weed tree sawdust Primary spawn
Base compares, and two kinds of pedigree seed culture mediums do 3 repetitions respectively.
7, bacteria: quiescent culture 10d in 25 ± 1 DEG C of lucifuge incubators, treats that mycelium front end forms smooth consistent mycelia edge
During line, the most recordable line is as mycelial growth rate start line.Then record a mycelia edge line every 24h, remember continuously
The numerical value of record 8d is as statistical data.
8, calculate Antrodia camphorata average growth rate on different culture media, compare and reach a conclusion, and Taking Pictures recording result.
9, experimental result is as follows:
Result of the test as depicted in figs. 1 and 2, it will be seen from figure 1 that Antrodia camphorata is in the activated inclined plane culture medium of Lignum cinnamomi camphorae wood flour
Long speed is significantly faster than common PDA inclined-plane, and mycelium length reaches 2.58775mm/d, and mycelia is the densest；And at common PDA
On inclined-plane, antrodia camphorata mycelium is sparse, and color is light, uneven, and long speed is slow, and only 0.504mm/d, part test tube to later stage is even
Stagnate the longest.
No matter figure it is seen that in test tube or in bacterium bag, the Antrodia camphorata long speed on Lignum cinnamomi camphorae wood flour pedigree seed culture medium is fast
In weed tree sawdust pedigree seed culture medium, in test tube, mycelium length is 2.1875mm/d, and in bacterium bag, mycelium length reaches 2.20625mm/d,
And mycelia is sturdy the densest；And on weed tree sawdust pedigree seed culture medium, antrodia camphorata mycelium is sparse, color is light, uneven, and long speed is slow
Slowly, in test tube, mycelium length is only 0.255mm/d, and in bacterium bag, mycelium length is 0.3025mm/d, and some is to the later stage very
The longest to stagnating.
Embodiment 2:
Experimental strain: Antrodia camphorata
Experimental procedure:
1, the preparation of activated inclined plane culture medium:
The raw material of every liter of culture medium: Rhizoma Solani tuber osi (peeling) 200 grams, glucose 20 grams, 20 grams of agar, 100g Lignum cinnamomi camphorae wood flour.
By (20～30min) to boil water to Rhizoma Solani tuber osi and Lignum cinnamomi camphorae wood flour, filtered through gauze, obtain filtrate, filtrate adds glucose and agar, so
1L is complemented to afterwards with water, 121 DEG C of sterilizing 30min, it is subsequently poured in test tube, is configured to the test tube slant training of 18mm × 180mm
Support base.
2, Antrodia camphorata mother plant tube activation: aseptically inoculate, cut from Antrodia camphorata original strain one piece of about 5mm × 5mm,
The strain block of thick about 2mm, proceeds in the activated inclined plane culture medium that step 1 prepares；Using conventional PDA culture medium as comparison.
Two kinds of culture medium do 5 repetitions respectively.
3, bacteria record: be placed in quiescent culture 8d in 28 ± 1 DEG C of lucifuge incubators, treats that mycelium front end forms smooth consistent bacterium
During silk edge line, the most recordable line is as mycelial growth rate start line.Then record a mycelia edge line every 24h,
The numerical value of record 8d is as statistical data continuously.
4, the preparation of pedigree seed culture medium:
(1) Lignum cinnamomi camphorae wood flour pedigree seed culture medium: based on total mass fraction 100%, by 44% Lignum cinnamomi camphorae wood flour, 44% cotton seed hulls, 10%
Wheat bran, 1% sucrose and 1% Gypsum Fibrosum composition.According to the above ratio, Lignum cinnamomi camphorae wood flour, cotton seed hulls, wheat bran, sucrose and Gypsum Fibrosum are mixed
After, add water moistening and mix thoroughly, water content 55-60%, using after fermenting one day.
(2) weed tree sawdust pedigree seed culture medium: based on total mass fraction 100%, by 44% common weed tree sawdust, 44% cotton seed hulls, 10%
Wheat bran, 1% sucrose and 1% Gypsum Fibrosum composition.According to the above ratio, Lignum cinnamomi camphorae wood flour, cotton seed hulls, wheat bran, sucrose and Gypsum Fibrosum are mixed
After, add water moistening and mix thoroughly, water content 55-60%, using after fermenting one day.
5, raw material subpackage sterilizing: Lignum cinnamomi camphorae wood flour pedigree seed culture medium and weed tree sawdust pedigree seed culture medium are distributed into 30mm × 250mm respectively
Teat glass and the polypropylene bacterium bag of 13cm × 27cm, install rear 121 DEG C of sterilizing 1h, material temperature be down to less than 25 DEG C standby.
6, inoculation: by the Antrodia camphorata strain transfer through step 2 activated inclined plane culture medium culturing to the glass examination processed through step 5
Pipe is with in the Lignum cinnamomi camphorae wood flour in polypropylene bacterium bag and weed tree sawdust pedigree seed culture medium, and every 18mm × 180mm test tube slant can connect
The test tube original seed 6-8 of 30mm × 250mm props up or the bacterium bag original seed 3-5 bag of 13cm × 27cm.Oppose with weed tree sawdust pedigree seed culture medium
According to, two kinds of pedigree seed culture mediums do 3 repetitions respectively.
7, bacteria: quiescent culture 10d in 25 ± 1 DEG C of lucifuge incubators, treats that mycelium front end forms smooth consistent mycelia edge
During line, the most recordable line is as mycelial growth rate start line.Then record a mycelia edge line every 24h, remember continuously
The numerical value of record 8d is as statistical data.
8, calculate Antrodia camphorata average growth rate on different culture media, compare and reach a conclusion, and Taking Pictures recording result.
9, experimental result is as follows:
Result of the test, as it is shown on figure 3, the Antrodia camphorata long speed on Lignum cinnamomi camphorae wood flour pedigree seed culture medium is faster than weed tree sawdust pedigree seed culture medium, reaches
To 2.4375mm/d, and mycelia is sturdy the densest；And on weed tree sawdust pedigree seed culture medium, antrodia camphorata mycelium is sparse, color is light, no
Uniformly, and long speed is only 0.4275mm/d, counts to later stage mycelia more and just stagnates not long.
Claims (2)
1. the manufacture method of an Antrodia camphorata original seed, it is characterised in that comprise the following steps: Antrodia camphorata is inoculated into activated inclined plane culture medium
On carry out cellar culture, then inoculate and on pedigree seed culture medium, carry out cellar culture, obtain Antrodia camphorata original seed；
Described activated inclined plane culture medium is to replace routine to contain with Lignum cinnamomi camphorae (Cinnamomum camphora (L.) Presl.) wood flour
Cinnamomum kanahirai hay trees bits in the activated inclined plane culture medium of Cinnamomum kanahirai hay tree (Cinnamomum kanehirai) wood flour, described Primary spawn
Base is to replace the Cinnamomum kanahirai hay trees bits in the routine pedigree seed culture medium containing Cinnamomum kanahirai hay trees bits with Lignum cinnamomi camphorae wood flour；
Described pedigree seed culture medium, based on total mass fraction 100%, by 44～78% Lignum cinnamomi camphorae wood flour, 0～44% cotton seed hulls, 10～20%
Wheat bran, 1% sucrose and 1% Gypsum Fibrosum composition.
Manufacture method the most according to claim 1, it is characterised in that described activated inclined plane culture medium every liter is so to prepare
: by boil water to 200g Rhizoma Solani tuber osi and 100g Lignum cinnamomi camphorae wood flour, cross leaching filtrate, in filtrate, then add 20g glucose
With 20g agar, it is settled to 1L with water.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310719336.9A CN103733881B (en) | 2013-12-23 | A kind of manufacture method of Antrodia camphorata original seed |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310719336.9A CN103733881B (en) | 2013-12-23 | A kind of manufacture method of Antrodia camphorata original seed |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103733881A CN103733881A (en) | 2014-04-23 |
| CN103733881B true CN103733881B (en) | 2016-11-30 |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101191120A (en) * | 2006-11-28 | 2008-06-04 | 蔡达铭 | Culture method for antrodia cinnamomea |
| TW200944120A (en) * | 2008-04-23 | 2009-11-01 | Qi-Xi Lian | Artificial cultivation method for antrodia camphorata |
| CN102047814A (en) * | 2010-10-25 | 2011-05-11 | 青岛农业大学 | Micro ventilation lime wood antrodia camphorate cultivation method |
| TW201119567A (en) * | 2009-12-02 | 2011-06-16 | Endemic Species Res Inst C O A | Method for incubating fruiting bodies of Antrodia cinnamomea |
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101191120A (en) * | 2006-11-28 | 2008-06-04 | 蔡达铭 | Culture method for antrodia cinnamomea |
| TW200944120A (en) * | 2008-04-23 | 2009-11-01 | Qi-Xi Lian | Artificial cultivation method for antrodia camphorata |
| TW201119567A (en) * | 2009-12-02 | 2011-06-16 | Endemic Species Res Inst C O A | Method for incubating fruiting bodies of Antrodia cinnamomea |
| CN102047814A (en) * | 2010-10-25 | 2011-05-11 | 青岛农业大学 | Micro ventilation lime wood antrodia camphorate cultivation method |
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Address after: 510070 No. 100 martyrs Middle Road, Guangdong, Guangzhou Patentee after: Institute of Microbiology, Guangdong Academy of Sciences Patentee after: GUANGDONG YUEWEI EDIBLE FUNGI TECHNOLOGY Co.,Ltd. Address before: No. 56, courtyard, No. 100, Xianlie Middle Road, Guangzhou, Guangdong 510070 Patentee before: GUANGDONG INSTITUTE OF MICROBIOLOGY (GUANGDONG DETECTION CENTER OF MICROBIOLOGY) Patentee before: GUANGDONG YUEWEI EDIBLE FUNGI TECHNOLOGY Co.,Ltd. |
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