CN103728418A - Analyzing method for researching parameters of architecture and spatial distribution of crop roots - Google Patents
Analyzing method for researching parameters of architecture and spatial distribution of crop roots Download PDFInfo
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- CN103728418A CN103728418A CN201310746739.2A CN201310746739A CN103728418A CN 103728418 A CN103728418 A CN 103728418A CN 201310746739 A CN201310746739 A CN 201310746739A CN 103728418 A CN103728418 A CN 103728418A
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Abstract
The invention relates to an analyzing method for researching the growth parameters and spatial distribution of crop roots, so as to visually analyze the space architecture of the roots. The analyzing method comprises the following steps: 1) precisely and completely sampling the crop roots, subdividing the soil profile according to the actual undisturbed distribution positions of the crop roots, washing the roots in each soil block unit, and storing the roots at low temperature; 2) scanning the roots by using a high resolution scanner, and storing the scanning results in the form of pictures; 3) analyzing the pictures of the root with WinRhizo software, so as to obtain the parameters of the roots such as length, diameter and surface area and reduce the error caused by manual measurement; 4) drawing a three-dimensional diagram of the spatial distribution of the roots by using Surfer software according to the parameters of the roots such as length, diameter and surface area; 5) clearly and precisely analyzing the spatial distribution and the architecture of the roots according to the three-dimensional diagram of the spatial distribution of the roots. The analyzing method is convenient to operate, and can precisely and accurately analyze the characteristic parameters and the spatial distribution characters of the roots.
Description
Technical field
Patent of the present invention relates to a kind of analytical approach of studying crop root structure parameters and space distribution.
Background technology
In soil, the form of root system and distribution not only affect the absorption to nutrient and moisture, and affect soil nutrient availability.Due to the complicacy of growing process, its growing environment height Spatial-Temporal Variability of root growth environment particularly, limited to a great extent the research of plant root growth process and soil course and understanding.In the existing research about crop root, a lot of work is all to cultivate with nutrient solution or silica sand the short term tests carrying out under controlled conditions, the relation that cannot really understand in the complete growth and development process of crop the growth of root system in soil and grow with overground part.Because root growth is in soil, cannot directly observe, study the root system of growing in field soil, will obtain root system with distinct methods.Common method have root bore method, whole take method and Monolith method etc. (
w.Methods of studying root systems[M] .New York:Springer, Berlin Heidelberg, 19791).The complexity of distinct methods is different, and the information of obtaining is also different.It is relatively simple that root bores method, can understand the long density of root of different soil, but due to obtained be local root system, so cannot understand the space distribution of whole root system in soil, the floristic root system research that is more suitable for being evenly distributed in soil in root system.For root system plant pockety in soil, must there be enough sampling spots and rational sample position can guarantee that result approaches actual conditions; Whole takes the form that method can be understood whole root system, and obtains the information that root is heavy and root is long, but workload is large, and in uprooting and washing root process, may lose the long lateral root of a large amount of decision roots.By whole take method obtain root system and thereafter wash root process in lost a large amount of lateral roots, the biomass of the lateral root of this part loss is very little, but very large to the long contribution of total root.Existing research is pointed out, destructiveness is uprooted and is washed root and can cause a large amount of radicula losses, loss amount can be up to 50% (Pallant E, Holmgren R A, Schuler G E et al.Using a fine root extraction device to quantify small diameter corn roots (>01025mm) in field soils[J] .Plant Soil, 1993,153:273-2791).By Monolith method, obtain root system and can understand the space distribution of root system in soil, and obtain the long density of the root of root system in different soils space and the heavy density of root, result approaches reality most, but workload is huge.Method of the present invention has been drawn the advantage of Monolith method, but efficiently solves the huge drawback of the method workload.By original position small cubes gradation solid space, gather root system sample, divide soil block to carry out scanning analysis to the root system of the soil sample of each stereo omnibearing, first the stereo-picture analysis software of Engineering application is applied to root system Study on spatial distribution again, characterize root system at soil space X, Y, the level of Z direction, vertical space distribute.
Summary of the invention
Patent of the present invention proposes a kind of analytical approach of studying crop root structure parameters and space distribution, the description that solves root system spatial distribution characteristic is fuzzyyer, focus mostly in the analysis of one dimension univers parameter, can not determine the problem of the concrete Root Parameters value of actual distribution position, root system field, and root system sampling procedure is loaded down with trivial details, process is more coarse, leaks root and falls the problem that root phenomenon is serious, has made up weak point of the prior art.
Technical scheme of the present invention is achieved in that
An analytical approach of studying crop root structure parameters and space distribution, comprises the following steps:
1) utilize little cube of original position to get root device, crop root is carried out accurately to comprehensively sampling, keep the original state distributing position of root system.The depth of soil of every 10cm is one deck, and the square soil block that the volume of take is 10cm*10cm*10cm is unit, and whole plant root is taken out, and takes soil total depth and is as the criterion with theoretical root length.Root system of plant in each soil block is chosen and cleaned, pack valve bag into and take back laboratory, low temperature is preserved; .
2) root system after cleaning is lain in clear glass dish, in glass disc, pour clear water into, the water yield just covers root system and is as the criterion, and guarantee between root system without intersecting, clear glass dish is placed on high-resolution scanner, root system is scanned, root system is scanned into picture form;
3) use WinRhizo software to analyze the picture of root system, the Root Parameters such as root length, root diameter and root surface area once can be analyzed, time saving and energy saving, the error occurring while reducing manual measurement;
4) according to Root Parameters such as root length, root diameter and root surface areas, with Surfer Software on Drawing root system space distribution three-dimensional plot, with X, Y-axis represents the coordinate of same solum horizontal direction, with Z axis, represent the size of Root Parameters, accurately express the distribution of root system in soil space;
5) draw out Root Distribution three-dimensional plot, spatial distribution characteristic and corresponding Root Parameters (XY represents the coordinate of root system in soil, and the size of Z value represents the size of Root Parameters) thereof that can clear accurate analysis root system
Beneficial effect of the present invention is: easy and simple to handle, save time, saving of labor, leakage root is few, can obtain comparatively accurate, comprehensive root system information, utilize spatial image analysis software to simulate, characterize intuitively the space distribution of root system each soil layer in space, can obtain root growth and development characteristic parameter and the space distribution configuration thereof in root system each mikey region in field, finally obtain root system steric configuration figure
Embodiment
Below the technical scheme in the embodiment of the present invention is clearly and completely described, obviously, described embodiment is only the present invention's part embodiment, rather than whole embodiment.Embodiment based in the present invention, those of ordinary skills, not making the every other embodiment obtaining under creative work prerequisite, belong to the scope of protection of the invention.
An analytical approach of studying crop root structure parameters and space distribution, comprises the following steps:
1) utilize and get root device, crop root is carried out accurately to comprehensively sampling, the original state distributing position that keeps root system, root system is cleaned, low temperature is preserved: during sampling according to the sample point coordinates of finishing in advance, the soil block of 10cmx10cm x10cm size of take is unit, makes the in the same size of itself and hollow cubic steel body 1.Every 10cm is one deck, determines size and the soil block number of every one deck Sampling Area according to the horizontal distribution size of root system.Original state characteristic distributions according to root system in soil, the cube of 10cm x10cm x10cm volume size of take is unit, whole root system piecemeal can be taken out, and finally be spliced into whole root structure.Can calculate the soil weight, soil moisture content, the long density of root, root system surface area density, system general length, root system total surface area and laterally with longitudinally distributing in soil thereof simultaneously;
2) application of high resolution scanner scans root system, root system is scanned into picture form (jpeg form): by non-overlapping being placed on transparency glass plate of root system after cleaning, splash into clear water, make it cover root system (guaranteeing that small radicula divides expansion), then root system is scanned into the picture that resolution is 300dpi, input computer, in order to carrying out root system analysis;
3) use WinRhizo root system software to analyze the picture of root system: by the picture input WinRhizo root system analysis software after root system scanning, the Root Parameters such as root length, root diameter and root surface area once can be analyzed, time saving and energy saving, to occur while reducing manual measurement error;
4) with Surfer Software on Drawing root system space distribution three-dimensional plot: according to Root Parameters such as root length, root diameter and root surface areas, while take field sampling, horizontal direction (directions X and Y-direction) is coordinate axis, take Root Parameters value as Z axis, Root Parameters is depicted as to three-dimensional isogram, accurately expresses the distribution of root system in soil space;
5) according to the Root Distribution three-dimensional plot of drawing out, the Root Parameters value in a certain definite site in soil space can be accurately expressed, the spatial distribution characteristic of root system in different soil and same soil layer varying level direction can be clearly analyzed accurately.
The foregoing is only preferred embodiment of the present invention, in order to limit the present invention, within the spirit and principles in the present invention not all, any modification of doing, be equal to replacement, improvement etc., within all should being included in protection scope of the present invention.
Claims (1)
1. an analytical approach of studying crop root structure parameters and space distribution, is characterized in that, comprises the following steps:
1) utilize and get root device, crop root is carried out accurately to comprehensively sampling, keep the original state distributing position of root system, root system is cleaned, low temperature is preserved;
2) application of high resolution scanner scans root system, and root system is scanned into picture form;
3) use WinRhizo software to analyze the picture of root system, the Root Parameters such as root length, root diameter and root surface area once can be analyzed, time saving and energy saving, the error occurring while reducing manual measurement;
4) according to Root Parameters such as root length, root diameter and root surface areas, with Surfer Software on Drawing root system space distribution three-dimensional plot, accurately express the distribution of root system in soil space;
5) draw out Root Distribution three-dimensional plot, spatial distribution characteristic and corresponding Root Parameters thereof that can clear accurate analysis root system.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105608735A (en) * | 2015-12-24 | 2016-05-25 | 北京农业信息技术研究中心 | Crop root space division method and system |
| CN106197286A (en) * | 2016-07-12 | 2016-12-07 | 江苏省农业科学院 | A kind of Root morphology assay method of fibrous root system plants |
| CN110021336A (en) * | 2017-07-25 | 2019-07-16 | 中国石油天然气股份有限公司 | Root system of plant vertical distribution model building method and device |
| CN112858300A (en) * | 2021-01-25 | 2021-05-28 | 西南民族大学 | Method for determining morphological structures of different degradation gradient plant roots of alpine meadow |
Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU2180103C2 (en) * | 2000-04-07 | 2002-02-27 | Галда Александр Васильевич | Method for extracting root samples of deep-rooted, preferably medicine, plants, apparatus for removing soil and ground during root sampling and apparatus for determining orientation and coordinates of arrangement of plant roots in space |
| CN1776357A (en) * | 2005-12-14 | 2006-05-24 | 华南农业大学 | In-situ measuring method of plant root system geometry |
| CN101574044A (en) * | 2009-06-05 | 2009-11-11 | 中国科学院植物研究所 | Method for dynamically researching in-situ root system |
| CN101658107A (en) * | 2009-09-18 | 2010-03-03 | 华南农业大学 | Method for in-situ dynamic observation and measurement of root system of plant |
| CN101923011A (en) * | 2010-06-29 | 2010-12-22 | 中国科学院新疆生态与地理研究所 | Three-dimensional sampling method applicable to root system research |
| CN102511365A (en) * | 2011-12-15 | 2012-06-27 | 上海市农业生物基因中心 | Method for measuring configuration and morphology of rice seedling root |
| CN102763573A (en) * | 2012-07-05 | 2012-11-07 | 华南农业大学 | Plant root system in-situ dynamic observation device, system and method under soil culture conditions |
| CN102937410A (en) * | 2012-11-20 | 2013-02-20 | 中国神华能源股份有限公司 | Ectotrophic mycorrhiza mycelium field in situ dynamic monitoring method in ecological environment reconnaissance |
| CN103141320A (en) * | 2013-03-27 | 2013-06-12 | 西北农林科技大学 | Automatic plant root system monitoring system |
-
2013
- 2013-12-31 CN CN201310746739.2A patent/CN103728418A/en active Pending
Patent Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU2180103C2 (en) * | 2000-04-07 | 2002-02-27 | Галда Александр Васильевич | Method for extracting root samples of deep-rooted, preferably medicine, plants, apparatus for removing soil and ground during root sampling and apparatus for determining orientation and coordinates of arrangement of plant roots in space |
| CN1776357A (en) * | 2005-12-14 | 2006-05-24 | 华南农业大学 | In-situ measuring method of plant root system geometry |
| CN101574044A (en) * | 2009-06-05 | 2009-11-11 | 中国科学院植物研究所 | Method for dynamically researching in-situ root system |
| CN101658107A (en) * | 2009-09-18 | 2010-03-03 | 华南农业大学 | Method for in-situ dynamic observation and measurement of root system of plant |
| CN101923011A (en) * | 2010-06-29 | 2010-12-22 | 中国科学院新疆生态与地理研究所 | Three-dimensional sampling method applicable to root system research |
| CN102511365A (en) * | 2011-12-15 | 2012-06-27 | 上海市农业生物基因中心 | Method for measuring configuration and morphology of rice seedling root |
| CN102763573A (en) * | 2012-07-05 | 2012-11-07 | 华南农业大学 | Plant root system in-situ dynamic observation device, system and method under soil culture conditions |
| CN102937410A (en) * | 2012-11-20 | 2013-02-20 | 中国神华能源股份有限公司 | Ectotrophic mycorrhiza mycelium field in situ dynamic monitoring method in ecological environment reconnaissance |
| CN103141320A (en) * | 2013-03-27 | 2013-06-12 | 西北农林科技大学 | Automatic plant root system monitoring system |
Non-Patent Citations (5)
| Title |
|---|
| ERIC PALLANT等: "Using a fine root extraction device to quantify small diameter corn roots (≧ 0.025 mm) in field soils", 《PLANT AND SOIL》 * |
| PATRICK ARMENGAUD等: "EZ-RHIZO: integrated software for the fast and accurate measurement of root system architecture", 《THE PLANT JOURNAL》 * |
| 张吴平等: "均质壤土下玉米根系三维空间分布的模拟与参数分析", 《土壤学报》 * |
| 李克新等: "作物根系原位观测识别新方法综述", 《浙江农业学报》 * |
| 李宏等: "枣树根系空间分布规律研究", 《河南农业科学》 * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105608735A (en) * | 2015-12-24 | 2016-05-25 | 北京农业信息技术研究中心 | Crop root space division method and system |
| CN105608735B (en) * | 2015-12-24 | 2018-07-06 | 北京农业信息技术研究中心 | Crop root space-division method and system |
| CN106197286A (en) * | 2016-07-12 | 2016-12-07 | 江苏省农业科学院 | A kind of Root morphology assay method of fibrous root system plants |
| CN110021336A (en) * | 2017-07-25 | 2019-07-16 | 中国石油天然气股份有限公司 | Root system of plant vertical distribution model building method and device |
| CN112858300A (en) * | 2021-01-25 | 2021-05-28 | 西南民族大学 | Method for determining morphological structures of different degradation gradient plant roots of alpine meadow |
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Application publication date: 20140416 |