CN103728272A - SPR (Surface Plasma Resonance) imaging detection device - Google Patents
SPR (Surface Plasma Resonance) imaging detection device Download PDFInfo
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- CN103728272A CN103728272A CN201310673822.1A CN201310673822A CN103728272A CN 103728272 A CN103728272 A CN 103728272A CN 201310673822 A CN201310673822 A CN 201310673822A CN 103728272 A CN103728272 A CN 103728272A
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Abstract
The embodiment of the invention discloses an SPR (Surface Plasma Resonance) imaging detection device which comprises a base, an optical platform, an optical prism, a light source, an image receiving device, and a synchronous scanning device, wherein the synchronous scanning device comprises a motor, a sliding block guide rail, a sliding block, a connecting rod group, an incidence arm, and a reflection arm; the connecting rod group comprises a first connecting rod, a second connecting rod, a third connecting rod, a fourth connecting rod, and an circular arc guide rail; the first connecting rod and the second connecting rod are connected with the sliding block respectively; the first connecting rod and the second connecting rod form a reversed V-shaped rod; the third connecting rod is connected with the other end of the first connecting rod, and the first connecting rod and the third connecting rod form a V-shaped rod; the fourth connecting rod is connected with the other end of the second connecting rod, and the second connecting rod and the fourth connecting rod form a V-shaped rod; the circular arc guide rail is in sliding connection with the joint of the first connecting rod and the third connecting rod and the joint of the second connecting rod and the fourth connecting rod; the joint of the first connecting rod and the third connecting rod and the joint of the second connecting rod and the fourth connecting rod are kept sliding symmetrically along the circular arc guide rail. Therefore, the detecting precision of the SPR imaging detection device is improved.
Description
Technical field
The present invention relates to imaging detection technology field, relate in particular to a kind of surface plasma resonance image-forming pick-up unit.
Background technology
Surface plasma body resonant vibration (Surface Plasmon Resonance, referred to as SPR) sensor is a kind of sensor that can detect optical property (as dielectric thickness, the refractive index etc.) slight change of testing medium, its core component is metal function layer.As certain incident angle, wavelength and horizontal magnetic (transverse magnetic, referred to as TM) incident light of polarization direction, after the coupling mechanisms such as prism, the interface excitation surface plasma-wave (Surface Plasmon Wave, referred to as SPW) of meeting between metal function layer and testing medium.When this surface plasma-wave launching efficiency is maximum, will produce SPR phenomenon, be that boundary reflection light intensity produces sharp-decay or polarization direction changes, thereby by detecting the variation of corresponding incident angle, incident wavelength, polarization direction or boundary reflection light intensity of SPR phenomenon, realize the detection to the optical property of near interface testing medium between metal function layer and testing medium.
The eighties in 20th century, spr sensor just starts for detection of near the variation of refractive index metal surface (Gordon, J.G.; Ernst, S.Surf.Sci.1980,101,499).Spr sensor was all widely used in fields such as drug screening, Biochemical Research in the last few years, become at present the important tool (Homola of qualitative and quantitative analysis biomolecular reaction (as kinetic parameter and detection substrate concentration), Chem.Rev.2008,108,462-493).
In prior art, utilize SPR principle, the method that detects near the variations in refractive index in metal surface has four kinds, comprising: angle detection, phase-detection, wavelength detect and intensity detection.Wherein, strength detecting method is that incident angle is fixed near intensity of reflected light minimum value, when the refractive index of check point generation biochemical reaction or check point changes, angle corresponding to intensity of reflected light minimum value changes, drive the fixedly variation of incident angle place intensity of illumination simultaneously, thereby by detecting fixed angle place, the variation of intensity of reflected light, obtain the information of biochemical reaction or variations in refractive index, be widely used in high-throughout SPR imaging and detect.But the accuracy of detection of SPR imaging detection device is lower in prior art.
Summary of the invention
For solving the problems of the technologies described above, the embodiment of the present invention provides a kind of surface plasma resonance image-forming pick-up unit, to improve the accuracy of detection of described surface plasma resonance image-forming pick-up unit.
For addressing the above problem, the embodiment of the present invention provides following technical scheme:
A surface plasma resonance image-forming pick-up unit, comprises pedestal, optical table, optical prism, light source and image received device, also comprises:
The synchronous scanning device that is positioned at described optical prism below, described synchronous scanning device comprises: motor; Be positioned at the slide block guide rail of described motor top; Be connected with described motor, under the drive of described motor, the slide block moving up and down along described slide block guide rail; Be connected with described slide block, under the drive of described slide block, the connection rod set of moving within being parallel to described base plane; The incident arm being connected with described connection rod set one end and the reflection arm being connected with the described connection rod set other end;
Wherein, described connection rod set comprises: the first connecting rod and the second connecting rod that are fixedly connected with described slide block respectively, and described first connecting rod and second connecting rod form reverse V-shaped bar; With the third connecting rod that the described first connecting rod other end is fixedly connected with, described first connecting rod and third connecting rod form V-type bar; The 4th connecting rod being fixedly connected with the described second connecting rod other end, described second connecting rod and the 4th connecting rod form V-type bar; With the arc guide rail that the tie point of tie point, second connecting rod and the 4th connecting rod of described first connecting rod and third connecting rod is slidably connected, the tie point of the tie point of described first connecting rod and third connecting rod, second connecting rod and the 4th connecting rod keeps symmetrical along described arc guide rail and slides.
Preferably, described first connecting rod, second connecting rod, third connecting rod and the 4th connecting rod are isometric.
Preferably, described light source comprises:
Semiconductor laser;
Emergent light to described semiconductor laser expands, and the collimating apparatus of collimation;
The polarization direction of the emergent light after collimation is adjusted to the polarizer of TM polarization;
Regulate the attenuator of the emergent light light intensity of the described polarizer;
Intercept the rectangular light hurdle of the emergent light center intensity uniform parts of described attenuator.
Preferably, described collimating apparatus comprises: a plurality of lens that the emergent light of described semiconductor laser is expanded.
Preferably, described light source comprises:
Produce the White LED of white emergent light;
The collimating apparatus that the emergent light of described White LED is collimated;
The polarization direction of the emergent light after collimation is adjusted to the polarizer of TM polarization;
Regulate the attenuator of the emergent light light intensity of the described polarizer;
The emergent light of described attenuator is carried out to the wave filter of filtering.
Preferably, described collimating apparatus is simple lens.
Preferably, described optical table and described optical prism seal by annulus.
Preferably, the position being fixedly connected with described optical prism on described optical table is provided with groove.
Preferably, on described pedestal, be provided with the tommy of calibration described optical prism position.
Preferably, described motor is stepper motor.
Compared with prior art, technique scheme has the following advantages:
The technical scheme that the embodiment of the present invention provides, can be by the mechanical drive advance amount of described motor, directly calculate described first connecting rod and the tie point of third connecting rod and the tie point of second connecting rod and the 4th connecting rod along the slide displacement of arc guide rail, again according to described slide displacement, calculate the angular displacement of described incident arm and reflection arm, can obtain the variation of described light source emergent light incident angle, improve the accuracy of detection of described surface plasma resonance image-forming pick-up unit.
And, in embodiments of the present invention, because described connection rod set and described arc guide rail are slidably connected, thereby the slip of described connection rod set can be fixed in the plane at described arc guide rail place, prevent that described connection rod set is perpendicular to described arc guide rail place rotation with in surface, thereby further improved the precision while calculating described light source emergent light incident angle variation, finally improved the accuracy of detection of described surface plasma resonance image-forming pick-up unit.
Accompanying drawing explanation
In order to be illustrated more clearly in the embodiment of the present invention or technical scheme of the prior art, to the accompanying drawing of required use in embodiment or description of the Prior Art be briefly described below, apparently, accompanying drawing in the following describes is some embodiments of the present invention, for those of ordinary skills, do not paying under the prerequisite of creative work, can also obtain according to these accompanying drawings other accompanying drawing.
Fig. 1 is the structural representation of SPR imaging detection device in prior art;
The structural representation of the surface plasma resonance image-forming pick-up unit that Fig. 2 provides for the embodiment of the present invention;
The partial structurtes schematic diagram that Fig. 3 is the surface plasma resonance image-forming pick-up unit that provides in one embodiment of the invention;
The partial structurtes schematic diagram that Fig. 4 is the surface plasma resonance image-forming pick-up unit that provides in another embodiment of the present invention;
The partial structurtes schematic diagram that Fig. 5 is the surface plasma resonance image-forming pick-up unit that provides in the embodiment of the present invention.
Embodiment
As shown in Figure 1, SPR imaging detection device of the prior art comprises:
Be fixed on the optical table 02 of described pedestal 01 upper end, side;
Be fixed on the optical prism 03 of described optical table 02 side;
Be positioned at the synchronous scanning device 04 of described optical prism 03 below, described synchronous scanning device 04 comprises: motor 041; Be positioned at the slide block guide rail 042 of described motor 041 top; The slide block 043 being connected with described motor 041; With the connection rod set 044 that described slide block 043 is connected, described connection rod set 044 comprises first connecting rod and the second connecting rod being fixedly connected with described slide block 043 respectively;
The incident arm 045 being connected with the described first connecting rod other end and the reflection arm 046 being connected with the described second connecting rod other end, wherein, described incident arm 045 and reflection arm 046 are relatively arranged on the both sides of described optical prism 03;
Be fixed on the light source 05 on described incident arm 045;
Be fixed on the CCD receiver 06 on described reflection arm 046.
SPR imaging detection device shown in Fig. 1 is when work, by slide block described in described driven by motor, along described slide block guide rail, move up and down, thereby drive the tie point of described first connecting rod and second connecting rod, perpendicular bisector along described optical prism moves up and down, thereby change described first connecting rod and second connecting rod subtended angle, change the subtended angle of described incident arm and reflection arm, realize the change of incident angle, and guarantee the light that described light source sends simultaneously, through described optical prism, all by described CCD receiver, received, be embodied as picture and detect.
But the accuracy of detection of above-mentioned SPR imaging detection device is lower.
Inventor studies discovery, this is that movement locus due to the tie point of the tie point of described incident arm and first connecting rod and reflection arm and second connecting rod is camber line, and the movement locus of described slide block is straight line, when calculating the variation of described light source emergent light incident angle, need first to convert the mechanical drive advance amount of described motor to bolt rotary amount, and then the amount of spin of described screw rod is converted to the angular turn amount of connection rod set, easy like this in long-term use procedure wearing and tearing and the dislocation due to screw rod produce return difference, reduce the accuracy of detection of described SPR imaging detection device.
And be subject to the impact of external shock, in the rotation process of described connection rod set, can not guarantee to be only positioned at the plane that is parallel to described pedestal completely, also may in the plane perpendicular to described pedestal, rotate, cause introducing error when calculating the variation of described light source emergent light incident angle, further reduce the accuracy of detection of described SPR imaging detection device.
In view of this, the embodiment of the present invention provides a kind of surface plasma resonance image-forming pick-up unit, comprises pedestal, optical table, optical prism, light source and image received device, in addition, also comprises:
The synchronous scanning device that is positioned at described optical prism below, described synchronous scanning device comprises: motor; Be positioned at the slide block guide rail of described motor top; Be connected with described motor, under the drive of described motor, the slide block moving up and down along described slide block guide rail; Be connected with described slide block, under the drive of described slide block, the connection rod set of moving within being parallel to described base plane; The incident arm being connected with described connection rod set one end and the reflection arm being connected with the described connection rod set other end;
Wherein, described connection rod set comprises: the first connecting rod and the second connecting rod that are fixedly connected with described slide block respectively, and described first connecting rod and second connecting rod form reverse V-shaped bar; With the third connecting rod that the described first connecting rod other end is fixedly connected with, described first connecting rod and third connecting rod form V-type bar; The 4th connecting rod being fixedly connected with the described second connecting rod other end, described second connecting rod and the 4th connecting rod form V-type bar; With the arc guide rail that the tie point of tie point, second connecting rod and the 4th connecting rod of described first connecting rod and third connecting rod is slidably connected, the tie point of the tie point of described first connecting rod and third connecting rod, second connecting rod and the 4th connecting rod keeps symmetrical along described arc guide rail and slides.
The surface plasma resonance image-forming pick-up unit that the embodiment of the present invention provides, can be by the mechanical drive advance amount of described motor, directly calculate described first connecting rod and the tie point of third connecting rod and the tie point of second connecting rod and the 4th connecting rod along the slide displacement of arc guide rail, again according to described slide displacement, the angular displacement of calculating described incident arm and reflection arm, can obtain the variation of described light source emergent light incident angle.As can be seen here, when the surface plasma resonance image-forming pick-up unit providing in utilizing the present embodiment detects, save and converted the mechanical drive advance amount of described motor to bolt rotary amount, and then the amount of spin of described screw rod is converted to the computation process of the angular turn amount of connection rod set, thereby avoided wearing and tearing and the dislocation due to screw rod in long-term use procedure to produce return difference, improved the accuracy of detection of described surface plasma resonance image-forming pick-up unit.
And, in embodiments of the present invention, because described connection rod set and described arc guide rail are slidably connected, thereby the slip of described connection rod set can be fixed in the plane at described arc guide rail place, prevent that described connection rod set is perpendicular to described arc guide rail place rotation with in surface, thereby further improved the precision while calculating described light source emergent light incident angle variation, finally improved the accuracy of detection of described surface plasma resonance image-forming pick-up unit.
For above-mentioned purpose of the present invention, feature and advantage can more be become apparent, below in conjunction with accompanying drawing, the specific embodiment of the present invention is described in detail.
Set forth detail in the following description so that fully understand the present invention.But the present invention can be different from alternate manner described here and implements with multiple, and those skilled in the art can do similar popularization without prejudice to intension of the present invention in the situation that.Therefore the present invention is not subject to the restriction of following public concrete enforcement.
As shown in Figure 2, the embodiment of the present invention provides a kind of surface plasma resonance image-forming pick-up unit, comprising: pedestal 1, optical table 2, optical prism 3, synchronous scanning device 4, light source 5 and image received device 6.
In one embodiment of the invention, described pedestal 1 is vertically placed, and its upper end, side level is fixed with optical table 2.The side of described optical table 2 is fixed with optical prism 3, seals between the two by annulus.Described optical prism 3 is equilateral triangle optical prism, and one angle vertically downward, is positioned on the vertical centering control axis of described optical prism 3.Under described optical prism 3, be provided with synchronous scanning device 4, described synchronous scanning device 4 comprises: motor 41; Be positioned at the slide block guide rail 42 of described motor 41 tops, described slide block guide rail 42 bottoms are connected with described motor 41, and described slide block guide rail 42 is fixed along the vertical centering control axis of described pedestal 1; Be connected with described motor 41, under the drive of described motor 41, the slide block 43 moving up and down along described slide block guide rail 42; Be connected with described slide block 43, under the drive of described slide block 43, in the connection rod set 44 that is parallel to described pedestal 1 flat in-plane moving; The incident arm 45 being connected with described connection rod set 44 one end and the reflection arm 46 being connected with described connection rod set 44 other ends, wherein, described incident arm 45 and reflection arm 46 are relatively arranged on the both sides of described optical prism 3.On described incident arm 45, be provided with light source 5; On described reflection arm 46, be provided with image received device 6.
Wherein, described connection rod set 44 comprises: the first connecting rod and the second connecting rod that are fixedly connected with described slide block 43 respectively, and described first connecting rod and second connecting rod form reverse V-shaped bar; With the third connecting rod that the described first connecting rod other end is fixedly connected with, described first connecting rod and third connecting rod form V-type bar; The 4th connecting rod being fixedly connected with the described second connecting rod other end, described second connecting rod and the 4th connecting rod form V-type bar; The arc guide rail 48 being slidably connected with the tie point of tie point, second connecting rod and the 4th connecting rod of described first connecting rod and third connecting rod.Wherein, described arc guide rail 48 is fixed on described pedestal 1, and its center of arc is positioned at the bottom center place of described optical prism 3; The tie point of the tie point of described first connecting rod and third connecting rod, second connecting rod and the 4th connecting rod keeps symmetrical along described arc guide rail 48 and slides.
In addition, as shown in Figure 3, the surface plasma resonance image-forming pick-up unit that the embodiment of the present invention provides also comprises: be positioned at the micro-array chip 8 of plane in described optical prism 3 levels, and be positioned at micro-measuring cell 9 on described micro-array chip 8 surfaces.Preferably, in one embodiment of the invention, fill with index-matching fluid the clearance between described optical prism 3 and micro-array chip 8.
When the surface plasma resonance image-forming pick-up unit that the embodiment of the present invention provides is worked, by described motor 41, drive described slide block 43 to move up and down along described slide block guide rail 42, thereby drive the connection rod set 44 being connected with described slide block 43, be parallel to the flat in-plane moving of described pedestal 1, and then the incident arm 45 that is connected with described connection rod set 44 one end of drive and the reflection arm 46 being connected with described connection rod set 44 other ends rotate centered by the bottom surface of described optical prism 3.Because be provided with light source 5 on described incident arm 45, on described reflection arm 46, be provided with image received device 6 again.When described incident arm 45 rotates centered by the bottom surface of described optical prism 3 with reflection arm 46, can be so that the rotation that described light source 5 and image received device 6 produce symmetry angle, guarantee that emergent light that described light source 5 sends is with different incident angles, from the left tilted surface of described optical prism 3, inject, the interface of optical prism 3 and described micro-array chip 8 described in directive, in described optical prism 3 and the interface of described micro-array chip 8, reflect, forming reflected light penetrates from the right tilted surface of described optical prism 3, full illumination is in the entrance port of described image collecting device 6, by described image collecting device 6, received, carry out imaging detection.
In this embodiment, described surface plasma resonance image-forming pick-up unit, can be by the mechanical drive advance amount of described motor 41, directly calculate first connecting rod and the tie point of third connecting rod and the slide displacement of the tie point of second connecting rod and the 4th connecting rod on arc guide rail 48 in described connection rod set 44, again according to described slide displacement, the angular displacement of calculating described incident arm 45 and reflection arm 46, can obtain the variation of described light source 5 emergent light incident angles.
As can be seen here, when the surface plasma resonance image-forming pick-up unit providing in utilizing the present embodiment detects, save and converted the mechanical drive advance amount of described motor to bolt rotary amount in prior art, and then the amount of spin of described screw rod is converted to the computation process of the angular turn amount of connection rod set, thereby avoided wearing and tearing and the dislocation due to screw rod in long-term use procedure to produce return difference, improved the accuracy of detection of described surface plasma resonance image-forming pick-up unit.
And, in embodiments of the present invention, because described connection rod set 44 is slidably connected with described arc guide rail 48, thereby the slip of described connection rod set 44 can be fixed in the plane at described arc guide rail 48 places, prevent that described connection rod set 44 is perpendicular to described arc guide rail 48 place rotation with in surfaces, thereby further improved the precision while calculating described light source 5 emergent light incident angles variation, finally improved the accuracy of detection of described surface plasma resonance image-forming pick-up unit.
Preferably, described first connecting rod, second connecting rod, third connecting rod and the 4th connecting rod are isometric.
In one embodiment of the invention, as shown in Figure 3, described light source 5 comprises: semiconductor laser 501; Emergent light to described semiconductor laser 501 expands, and the collimating apparatus 502 of collimation; The polarization direction of the emergent light after collimation is adjusted to the polarizer 503 of TM polarization; Regulate the attenuator 504 of the emergent light light intensity of the described polarizer 503; Intercept the rectangular light hurdle 505 of the emergent light center intensity uniform parts of described attenuator 504.
The course of work of described light source 5 comprises: described semiconductor laser 501 produces the emergent light of single wavelength; Utilize the emergent light of 502 pairs of described semiconductor lasers 501 of described collimating apparatus to collimate, guarantee that the incident angle of entire light is consistent; Utilize the described polarizer 503 that the polarization direction of the emergent light after collimation is adjusted to TM polarization; Utilize described attenuator 504 to control emergent light intensity, prevent that saturated phenomenon from appearring in image received device 6 when receiving reflected light; Utilize described rectangular light hurdle 505 that the center intensity uniform parts of the emergent light through attenuator 504 is intercepted, guarantee the light intensity of beam incident optical prism 3, consistent within the scope of covering space.
It should be noted that, in this embodiment, described surface plasma resonance image-forming pick-up unit also comprises: the lens 506 that are positioned at described reflection arm 46, described lens 506 are for collecting the reflected light by optical prism 3 and micro-array chip 8 generation of interfaces, and by the reflected light directive image received device 6 of collecting; And described image received device 6 is for the emergent light of lens 506 being carried out to opto-electronic conversion, be embodied as the collection of picture detection signal.
In this embodiment, the monochromaticity of described semiconductor laser 501 is relatively good, can directly produce the emergent light of single wavelength, be conducive to guarantee, in spatial dimension that incident light covers at it, keep the high uniformity of incident intensity, and the relatively good tune of light path.
But, because described SPR imaging detection device is when working, require within the scope of incident light institute covering space, image received device 6 can receive the signal that can detect, thereby the emergent light that needs semiconductor laser 501 has larger optical intensity density, described semiconductor laser 501 can be exported larger power.And the output power of described semiconductor laser 501 is larger, cost is higher, thereby causes the cost of SPR imaging detection device described in this embodiment higher.
And, because the beam divergence angle of semiconductor laser 501 is less, be difficult to produce larger illumination range, the coverage that is its emergent light is less, therefore, needs to comprise a plurality of lens that the emergent light of described semiconductor laser 501 is expanded in described collimating apparatus 502, emergent light to described semiconductor laser first expands, expand the coverage of described emergent light, then collimate, guarantee that incident angle is consistent.
But, because the spectral distribution of laser is Gaussian distribution, after the emergent light of described semiconductor laser 501 is expanded, can produce comparatively significantly intensity of illumination phenomenon pockety, be that the marginal portion of described emergent light and the core deviation of described emergent light are larger, therefore, need in rectangular light hurdle 505 intercepting outgoing beams, the part of center intensity distribution uniform, thus cause described in this embodiment in SPR imaging detection device the size of light path system and regulate difficulty larger.
In order to reduce the cost of described surface plasma body resonant vibration (SPR) imaging detection device, and the size of dwindling described surface plasma resonance image-forming pick-up unit, in another embodiment of the present invention, as shown in Figure 4, described light source 5 comprises: the White LED 511 that produces white emergent light; The collimating apparatus 512 that the emergent light of described White LED 511 is collimated; The polarization direction of the emergent light after collimation is adjusted to the polarizer 513 of TM polarization; Regulate the attenuator 514 of the emergent light light intensity of the described polarizer 513; The emergent light of described attenuator 514 is carried out to the wave filter 515 of filtering.
Incident direction and wavelength incomplete same due to each light in the emergent light of White LED 511 generations, and light source requirements in surface plasma resonance image-forming pick-up unit is within the scope of its light beam covering space, keep the high uniformity of incident angle, polarization direction and intensity.Therefore, the light source 5 that the embodiment of the present invention provides, when work, when described White LED 511 produces after emergent light, need to, first through collimating apparatus 512 collimations, guarantee the consistent of described emergent light incident angle; The recycling polarizer 513 is adjusted to TM polarization by the polarization direction of the emergent light after collimation, so that the plasma of metal surface in excitating surface plasma resonance image-forming pick-up unit forms evanescent wave.Then, utilize attenuator 514 to regulate the intensity of illumination of the described polarizer 513 emergent lights, to prevent that image received device 6 in surface plasma resonance image-forming pick-up unit from, when receiving reflected light, occurring saturated phenomenon; Finally, utilize the emergent light of 515 pairs of described attenuators 514 of wave filter to carry out filtering, forming incident direction consistent, and thering is the emergent light of single wavelength.
Because the cost of high-power White LED 511 will be much smaller than the cost of high power semiconductor lasers 501, therefore the light source cost of the surface plasma resonance image-forming pick-up unit providing in the embodiment of the present invention is lower, thereby reduced the cost of described surface plasma resonance image-forming pick-up unit.
In one embodiment of the invention, described wave filter 515 can be filter plate, but the present invention do not limit this, as long as can play filter effect, obtains the emergent light of single wavelength.
It should be noted that, because the illumination range of White LED 511 emergent lights is larger, therefore, in one embodiment of the invention, described collimating apparatus 512 can be a simple lens, the emergent light of described White LED 511 is become to directional light, thereby the incident angle that guarantees described emergent light is consistent, and without a plurality of lens of recycling, the emergent light of described White LED 511 is expanded, thereby simplified the light-source structure of described surface plasma resonance image-forming pick-up unit, dwindled the size of described surface plasma resonance image-forming pick-up unit, also reduced the difficulty of the optical path adjusting in described surface plasma resonance image-forming pick-up unit.
Also it should be noted that, because the spectral distribution of the emergent light of White LED 511 is comparatively even, the intensity of illumination that is the emergent light of described White LED 511 distributes comparatively even, therefore, in one embodiment of the invention, described light source can not comprise rectangular light hurdle, thereby has further simplified the light-source structure of described surface plasma resonance image-forming pick-up unit, has reduced the difficulty of the optical path adjusting in described light source.
As shown in Figure 5, in one embodiment of the invention, the position being fixedly connected with described optical prism 3 on described optical table 2 is provided with groove 21, so that the installation of described annulus 22 can also be played the effect that prevents fluid seepage.In another embodiment of the present invention, on described pedestal 1, be also provided with tommy 7, so that fixing described optical prism 3 is calibrated two of described optical prism 3 lower surfaces.Of the present invention, have in an embodiment, described motor 41 is preferably stepper motor, and described image collecting device is preferably CCD receiver.
In sum, the surface plasma resonance image-forming pick-up unit that the embodiment of the present invention provides, can be by the mechanical drive advance amount of described motor, directly calculate described first connecting rod and the tie point of third connecting rod and the tie point of second connecting rod and the 4th connecting rod along the slide displacement of arc guide rail, again according to described slide displacement, the angular displacement of calculating described incident arm and reflection arm, can obtain the variation of described light source emergent light incident angle.As can be seen here, when the surface plasma resonance image-forming pick-up unit providing in utilizing the present embodiment detects, save and converted the mechanical drive advance amount of described motor to bolt rotary amount, and then the amount of spin of described screw rod is converted to the computation process of the angular turn amount of connection rod set, thereby avoided wearing and tearing and the dislocation due to screw rod in long-term use procedure to produce return difference, improved the accuracy of detection of described surface plasma resonance image-forming pick-up unit.
And, in embodiments of the present invention, because described connection rod set and described arc guide rail are slidably connected, thereby the slip of described connection rod set can be fixed in the plane at described arc guide rail place, prevent that described connection rod set is perpendicular to described arc guide rail place rotation with in surface, thereby further improved the precision while calculating described light source emergent light incident angle variation, finally improved the accuracy of detection of described surface plasma resonance image-forming pick-up unit.
In this instructions, various piece adopts the mode go forward one by one to describe, and each part stresses is the difference with other parts, between various piece identical similar part mutually referring to.
Above-mentioned explanation to the disclosed embodiments, makes professional and technical personnel in the field can realize or use the present invention.To the multiple modification of these embodiment, will be apparent for those skilled in the art, General Principle as defined herein can, in the situation that not departing from the spirit or scope of the present invention, realize in other embodiments.Therefore, the present invention will can not be restricted to embodiment illustrated herein, but will meet the widest scope consistent with principle disclosed herein and features of novelty.
Claims (10)
1. a surface plasma resonance image-forming pick-up unit, comprises pedestal, optical table, optical prism, light source and image received device, it is characterized in that, also comprises:
The synchronous scanning device that is positioned at described optical prism below, described synchronous scanning device comprises: motor; Be positioned at the slide block guide rail of described motor top; Be connected with described motor, under the drive of described motor, the slide block moving up and down along described slide block guide rail; Be connected with described slide block, under the drive of described slide block, the connection rod set of moving within being parallel to described base plane; The incident arm being connected with described connection rod set one end and the reflection arm being connected with the described connection rod set other end;
Wherein, described connection rod set comprises: the first connecting rod and the second connecting rod that are fixedly connected with described slide block respectively, and described first connecting rod and second connecting rod form reverse V-shaped bar; With the third connecting rod that the described first connecting rod other end is fixedly connected with, described first connecting rod and third connecting rod form V-type bar; The 4th connecting rod being fixedly connected with the described second connecting rod other end, described second connecting rod and the 4th connecting rod form V-type bar; With the arc guide rail that the tie point of tie point, second connecting rod and the 4th connecting rod of described first connecting rod and third connecting rod is slidably connected, the tie point of the tie point of described first connecting rod and third connecting rod, second connecting rod and the 4th connecting rod keeps symmetrical along described arc guide rail and slides.
2. surface plasma resonance image-forming pick-up unit according to claim 1, is characterized in that, described first connecting rod, second connecting rod, third connecting rod and the 4th connecting rod are isometric.
3. surface plasma resonance image-forming pick-up unit according to claim 1, is characterized in that, described light source comprises:
Semiconductor laser;
Emergent light to described semiconductor laser expands, and the collimating apparatus of collimation;
The polarization direction of the emergent light after collimation is adjusted to the polarizer of TM polarization;
Regulate the attenuator of the emergent light light intensity of the described polarizer;
Intercept the rectangular light hurdle of the emergent light center intensity uniform parts of described attenuator.
4. surface plasma resonance image-forming pick-up unit according to claim 3, is characterized in that, described collimating apparatus comprises: a plurality of lens that the emergent light of described semiconductor laser is expanded.
5. surface plasma resonance image-forming pick-up unit according to claim 1, is characterized in that, described light source comprises:
Produce the White LED of white emergent light;
The collimating apparatus that the emergent light of described White LED is collimated;
The polarization direction of the emergent light after collimation is adjusted to the polarizer of TM polarization;
Regulate the attenuator of the emergent light light intensity of the described polarizer;
The emergent light of described attenuator is carried out to the wave filter of filtering.
6. surface plasma resonance image-forming pick-up unit according to claim 5, is characterized in that, described collimating apparatus is simple lens.
7. according to the surface plasma resonance image-forming pick-up unit described in claim 1-6 any one, it is characterized in that, described optical table and described optical prism seal by annulus.
8. surface plasma resonance image-forming pick-up unit according to claim 7, is characterized in that, the position being fixedly connected with described optical prism on described optical table is provided with groove.
9. according to the surface plasma resonance image-forming pick-up unit described in claim 1-6 any one, it is characterized in that, on described pedestal, be provided with the tommy of calibration described optical prism position.
10. according to the surface plasma resonance image-forming pick-up unit described in claim 1-6 any one, described motor is stepper motor.
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| CN201310673822.1A CN103728272B (en) | 2013-12-11 | 2013-12-11 | Surface plasma resonance image-forming pick-up unit |
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| CN109799209A (en) * | 2019-01-31 | 2019-05-24 | 马佑桥 | A kind of simplification glass prism SPR excitation apparatus based on parabolic reflector |
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