CN103720646A - Enriched-Se isatis root polysaccharide oral liquid and preparation method thereof - Google Patents
Enriched-Se isatis root polysaccharide oral liquid and preparation method thereof Download PDFInfo
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- CN103720646A CN103720646A CN201310662126.0A CN201310662126A CN103720646A CN 103720646 A CN103720646 A CN 103720646A CN 201310662126 A CN201310662126 A CN 201310662126A CN 103720646 A CN103720646 A CN 103720646A
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Abstract
The invention relates to an enriched-Se isatis root polysaccharide oral liquid. Firstly, isatis roots, sodium chloride and water are mixed and heated to extract isatis root polysaccharide, then the collected isatis root polysaccharide and a selenium agent perform a catalytic reaction, and lastly, a reaction product is mixed with sulfactol and the water to obtain the finished product. The enriched-Se isatis root polysaccharide oral liquid has the effects of both the isatis root polysaccharide and organic Se, which are mutually combined and simultaneously act on an organism, can further improve the immunity ability of the organism; in the clinic practical application, the enriched-Se isatis root polysaccharide oral liquid avoids that poultry suddenly die under the state of lacking the Se and abnormal immunity, so as to lower farmers' economical loss.
Description
Technical field
The invention belongs to Banlangen Polysaccharide deep processing field, especially a kind of rich selenium Banlangen Polysaccharide oral liquid and preparation method thereof.
Background technology
Indigo-blue of Radix Isatidis another name, Isatis indigotica Fort (Indigofera tinctoria L, Baphicanthus cusia (nees) Brem. Polygonum tinctorium Ait) root and Radix Clerodenri Cyrtophylli, be the dry root of plant Isatis indigotica Fort. or careless Folium Isatidis, is elongated cylindrical, and surface is light yellow, coarse, has vertical wrinkle and horizontal spot, and it is Chinese medicine, has the function of heat-clearing and toxic substances removing, removing heat from blood sore-throat relieving.For diseases such as purple dark, the mumps of maculae caused by virulent heat pathogen, crimson tongue, sore throat, scarlet fever, major part pestilence, erysipelas, carbuncle.In the numerous compositions of Radix Isatidis, Banlangen Polysaccharide is one of main chemical composition, and research shows, it has immunoregulation capability, effect for reducing blood fat, antivirus action and effect of scavenging radical.
Organic selenium is a kind of Methionine metabolism approach metabolism of following, participate in the synthetic of albumen, easily in tissue, store, absorb, if the effect of Banlangen Polysaccharide and organic selenium can be mutually combined, will form and a kind ofly can bring into play the facilitation of organic selenium to body, can embody again the multifunctional product of Banlangen Polysaccharide effect.
Summary of the invention
The object of the invention is to overcome the deficiencies in the prior art, taking convenience is provided, is easy to a kind of rich selenium Banlangen Polysaccharide oral liquid produced.
The technical scheme that the present invention takes is:
A rich selenium Banlangen Polysaccharide oral liquid, is characterized in that: comprise the Radix Isatidis selenium polysaccharide of 1～20 part, sodium thiosulfate and the purified water of the former weight 0.2～0.5%.
And the preparation method of described Radix Isatidis selenium polysaccharide comprises the following steps:
Prepare Banlangen Polysaccharide
(1) Radix Isatidis and water are added in Low Temperature Thermal reflux, extract, tank by weight 1:5～15, temperature conditions is 70 degrees Celsius, and pressure is 0.25 MPa;
(2) the sodium chloride that adds Radix Isatidis and water gross weight 0.1～0.5%, then rises to 45～75 degrees Celsius by temperature in extraction pot and extracts, and extraction time is 2～8 hours
(3) collect extracting solution, under the condition of 0.3 MPa, carry out concentrating under reduced pressure, until relative density is 1.1～1.35 to stop;
(4) step product is (3) washed by 95% ethanol or absolute ether, centrifugal rear collection supernatant under per minute 500～1500 conditions that turn then, then supernatant is made to Banlangen Polysaccharide after by 120 degrees Celsius of heated-air circulation oven inner dryings.
Prepare Radix Isatidis selenium polysaccharide
(1) Banlangen Polysaccharide and selenizing agent are added in reactor by the weight ratio of 1:0.2-0.85 jointly;
(2) reacting by heating still, makes temperature in it rise to 40～65 degrees Celsius, adds catalyst;
(3) centrifugation step product (2) under per minute 500～1500 conditions that turn, then adsorb with the macroporous resin of 100～500 nanometers;
(4) step macroporous resin is (3) washed with absolute ether, the product of eluting is through centrifugal under per minute 500～1500 conditions that turn, and gleanings makes Radix Isatidis selenium polysaccharide after by 120 degrees Celsius of heated-air circulation oven inner dryings.
And, prepare Radix Isatidis selenium polysaccharide step (2) described catalyst be rare nitric acid or barium chloride, the weight ratio of catalyst and selenizing agent is 0.2～0.45:1, the catalytic reaction time is 2～12 hours.
Another object of the present invention is to provide a kind of preparation method of rich selenium Banlangen Polysaccharide oral liquid, it is characterized in that: comprise the following steps:
(1) Radix Isatidis selenium polysaccharide is mixed with sodium thiosulfate, add in Agitation Tank;
(2) add again purified water, stir 15～30 minutes;
(3) by 0.45 micron of filtration for step product (2), after subpackage, make finished product.
Advantage of the present invention and good effect are:
In the present invention, first Radix Isatidis and sodium chloride, water Hybrid Heating are extracted to Banlangen Polysaccharide, then the Banlangen Polysaccharide of collection and selenizing agent are carried out to catalytic reaction, last reactant makes finished product after being mixed with sodium thiosulfate and water again.Finished product possesses the double effects of Banlangen Polysaccharide and organic selenium, and the two mutually combines, and acts on body simultaneously, energy is the immunocompetence of enhancing body further, in clinical practice is used, avoided poultry in the sudden death lacking under selenium and dysimmunity state, reduced raiser's economic loss.
The specific embodiment
Below in conjunction with embodiment, the present invention is further described, and following embodiment is illustrative, is not determinate, can not limit protection scope of the present invention with following embodiment.
A rich selenium Banlangen Polysaccharide oral liquid, is characterized in that: comprise the Radix Isatidis selenium polysaccharide of 1～20 part, sodium thiosulfate and the purified water of the former weight 0.2～0.5%.
Wherein, the Radix Isatidis selenium polysaccharide preparation method in rich selenium Banlangen Polysaccharide oral liquid comprises the following steps:
Prepare Banlangen Polysaccharide
(1) Radix Isatidis and water are added in Low Temperature Thermal reflux, extract, tank by weight 1:5～15, temperature conditions is 70 degrees Celsius, and pressure is 0.25 MPa;
(2) the sodium chloride that adds Radix Isatidis and water gross weight 0.1～0.5%, then rises to 45～75 degrees Celsius by temperature in extraction pot and extracts, and extraction time is 2～8 hours
(3) collect extracting solution, under the condition of 0.3 MPa, carry out concentrating under reduced pressure, until relative density is 1.1～1.35 to stop;
(4) step product is (3) washed by 95% ethanol or absolute ether, centrifugal rear collection supernatant under per minute 500～1500 conditions that turn then, then supernatant is made to Banlangen Polysaccharide after by 120 degrees Celsius of heated-air circulation oven inner dryings.
Prepare Radix Isatidis selenium polysaccharide
(1) Banlangen Polysaccharide and selenizing agent are added in reactor by the weight ratio of 1:0.2-0.85 jointly;
(2) reacting by heating still, makes temperature in it rise to 40～65 degrees Celsius, adds the rare nitric acid of catalyst or barium chloride, and the weight ratio of catalyst and selenizing agent is 0.2～0.45:1, catalytic reaction 2～12 hours;
(3) centrifugation step product (2) under per minute 500～1500 conditions that turn, then adsorb with the macroporous resin of 100～500 nanometers;
(4) step macroporous resin is (3) washed with absolute ether, the product of eluting is through centrifugal under per minute 500～1500 conditions that turn, and gleanings makes Radix Isatidis selenium polysaccharide after by 120 degrees Celsius of heated-air circulation oven inner dryings.
The preparation method of above-mentioned rich selenium Banlangen Polysaccharide oral liquid comprises the following steps:
(1) Radix Isatidis selenium polysaccharide is mixed with sodium thiosulfate, add in Agitation Tank;
(2) add again purified water, stir 15～30 minutes;
(3) by 0.45 micron of filtration for step product (2), after subpackage, make finished product.
Embodiment 1
1. prepare Banlangen Polysaccharide
(1) 50 kilograms of Radix Isatidis and 250 kg of water (weight ratio 1:5) are added in Low Temperature Thermal reflux, extract, tank jointly, temperature conditions is 70 degrees Celsius, and pressure is 0.25 MPa;
(2) add 0.3 kilogram of sodium chloride (Radix Isatidis and water gross weight 0.1%), then temperature in extraction pot is risen to 45 degrees Celsius and extract, extraction time is 8 hours;
(3) collect extracting solution, under the condition of 0.3 MPa, carry out concentrating under reduced pressure, until relative density is 1.1 to stop;
By step product (3) by 95% washing with alcohol of 3 times, centrifugal rear collection supernatant under per minute 500～1500 conditions that turn then, then supernatant is made to Banlangen Polysaccharide after by 120 degrees Celsius of heated-air circulation oven inner dryings.
2. prepare Radix Isatidis selenium polysaccharide
(1) 10 kilograms of Banlangen Polysaccharides and 2 kilograms of triphenyl selenizing phosphines (1:0.2) are added in reactor jointly;
(2) reacting by heating still, makes temperature in it rise to 40 degrees Celsius, the rare nitric acid of catalyst that to add with selenizing agent weight ratio be 1:0.2, catalytic reaction 2 hours;
(3) centrifugation step product (2) under per minute 500 conditions that turn, then adsorb with the macroporous resin of 100 nanometers;
(4) step macroporous resin is (3) washed with absolute ether, the product of eluting is through centrifugal under per minute 500 conditions that turn, and gleanings makes Radix Isatidis selenium polysaccharide after by 120 degrees Celsius of heated-air circulation oven inner dryings.
Prepare Radix Isatidis selenium polysaccharide oral liquid
By 20 kilograms of Radix Isatidis selenium polysaccharides and 0.04 kilogram of sodium thiosulfate (for Radix Isatidis selenium polysaccharide weight 0.2%) mix, add in Agitation Tank;
(2) add again 100 liters of purified water, stir 15 minutes;
(3) by 0.45 micron of filtration for step product (2), after subpackage, make finished product.
Embodiment 2
1. prepare Banlangen Polysaccharide
(1) 60 kilograms of Radix Isatidis and 900 kg of water (weight ratio 1:15) are added in Low Temperature Thermal reflux, extract, tank jointly, temperature conditions is 70 degrees Celsius, and pressure is 0.25 MPa;
(2) add 4.8 kilograms of sodium chloride (Radix Isatidis and water gross weight 0.5%), then temperature in extraction pot is risen to 75 degrees Celsius and extract, extraction time is 2 hours;
(3) collect extracting solution, under the condition of 0.3 MPa, carry out concentrating under reduced pressure, until relative density is 1.35 to stop; (the actual conditions of concentrating under reduced pressure?)
(4) by step product (3), the absolute ether by 5 times washs, centrifugal rear collection supernatant under per minute 1500 conditions that turn then, then supernatant is made to Banlangen Polysaccharide after by 120 degrees Celsius of heated-air circulation oven inner dryings.
2. prepare Radix Isatidis selenium polysaccharide
(1) 20 kilograms of Banlangen Polysaccharides and 17 kilograms of pyridine-dichloro oxygen selenium (1:0.85) are added in reactor jointly;
(2) reacting by heating still, makes temperature in it rise to 65 degrees Celsius, the catalyst barium chloride that to add with selenizing agent weight ratio be 1:0.45, catalytic reaction 12 hours;
(3) centrifugation step product (2) under per minute 1500 conditions that turn, then adsorb with the macroporous resin of 500 nanometers;
(4) step macroporous resin is (3) washed with absolute ether, the product of eluting is through centrifugal under per minute 1500 conditions that turn, and gleanings makes Radix Isatidis selenium polysaccharide after by 120 degrees Celsius of heated-air circulation oven inner dryings.
Prepare Radix Isatidis selenium polysaccharide oral liquid
By 15 kilograms of Radix Isatidis selenium polysaccharides and 0.075 kilogram of sodium thiosulfate (for Radix Isatidis selenium polysaccharide weight 0.5%) mix, add in Agitation Tank;
(2) add again 100 liters of purified water, stir 30 minutes;
(3) by 0.45 micron of filtration for step product (2), after subpackage, make finished product.
Embodiment 3
1. prepare Banlangen Polysaccharide
(1) 100 kilograms of Radix Isatidis and 1000 kg of water (weight ratio 1:10) are added in Low Temperature Thermal reflux, extract, tank jointly, temperature conditions is 70 degrees Celsius, and pressure is 0.25 MPa;
(2) add 3.3 kilograms of sodium chloride (Radix Isatidis and water gross weight 0.3%), then temperature in extraction pot is risen to 75 degrees Celsius and extract, extraction time is 5 hours;
(3) collect extracting solution, under the condition of 0.3 MPa, carry out concentrating under reduced pressure, until relative density is 1.2 to stop;
(4) by step product (3), the absolute ether by 4 times washs, centrifugal rear collection supernatant under per minute 1200 conditions that turn then, then supernatant is made to Banlangen Polysaccharide after by 120 degrees Celsius of heated-air circulation oven inner dryings.
2. prepare Radix Isatidis selenium polysaccharide
(1) 20 kilograms of Banlangen Polysaccharides and 11 kilograms of pyridine-dichloro oxygen selenium (1:0.55) are added in reactor jointly;
(2) reacting by heating still, makes temperature in it rise to 65 degrees Celsius, the catalyst barium chloride that to add with selenizing agent weight ratio be 1:0.35, catalytic reaction 8 hours;
(3) centrifugation step product (2) under per minute 1200 conditions that turn, then adsorb with the macroporous resin of 300 nanometers;
(4) step macroporous resin is (3) washed with absolute ether, the product of eluting is through centrifugal under per minute 1200 conditions that turn, and gleanings makes Radix Isatidis selenium polysaccharide after by 120 degrees Celsius of heated-air circulation oven inner dryings.
Prepare Radix Isatidis selenium polysaccharide oral liquid
By 30 kilograms of Radix Isatidis selenium polysaccharides and 0.12 kilogram of sodium thiosulfate (for Radix Isatidis selenium polysaccharide weight 0.4%) mix, add in Agitation Tank;
(2) add again 100 liters of purified water, stir 20 minutes;
(3) by 0.45 micron of filtration for step product (2), after subpackage, make finished product.
When prepared by Banlangen Polysaccharide, add the yield that can increase significantly Banlangen Polysaccharide after sodium chloride, the results are shown in Table 1:
Table 1: the relation of Banlangen Polysaccharide yield and sodium chloride addition
As seen from the table, in Banlangen Polysaccharide preparation process, add 0.1～0.5% sodium chloride, can improve the yield of Banlangen Polysaccharide, wherein add sodium chloride addition can improve Banlangen Polysaccharide yield 17% when 0.4～0.5% scope.
The ratio of Radix Isatidis selenium polysaccharide in feeding water pressed example 1 for 100ml/300L, and example 2 is 100ml/300L, and example 3 is 100ml/300L, is respectively test group 1, test group 2, test group 3, and matched group is feeding not.
Feed 30 days, record the initial body weight of every pig, whole opisthosoma weight, take average weight gain, heavy, the average feedstuff-meat ratio of average feed consumption, mortality rate as detecting index.Table 2 be Radix Isatidis selenium polysaccharide oral liquid to pig weight increment test result, table 3 is that Radix Isatidis selenium polysaccharide oral liquid is to pig resistance against diseases result of the test.
Table 2: Radix Isatidis selenium polysaccharide oral liquid is to pig weight increment test result
Table 3: Radix Isatidis selenium polysaccharide oral liquid is to pig resistance against diseases result of the test
The ratio of Radix Isatidis selenium polysaccharide in feeding water pressed example 1 for 100ml/300L, and example 2 is 100ml/300L, and example 3 is 100ml/300L, is respectively test group 1, test group 2, test group 3, and matched group is feeding not.。Feed 30 days, record turns group Gain weight of before and after test object.Table 4 is that Radix Isatidis selenium polysaccharide oral liquid is to pig anti-stress result of the test.
Table 4: Radix Isatidis selenium polysaccharide oral liquid is to pig anti-stress result of the test
Test is divided into 4 groups at random, one group is matched group, by normal feeding manner, raise, other 3 groups is test group, in feeding process, add Radix Isatidis selenium polysaccharide oral liquid, by the mixed drink of 100ml/300L water, matched group broiler is by normal aquaculture model be correlated with vaccine immunity and medication, feed 30 days, record weightening finish and the mortality rate of broiler.Table 5 is that Radix Isatidis selenium polysaccharide oral liquid is to broiler growth result of the test.
Table 5: Radix Isatidis selenium polysaccharide oral liquid is to broiler growth result of the test
By infrared heating, make ambient temperature reach 32 ℃, and be incubated by thermostat, as high temperature matched group and test group experimental condition.The ratio of Radix Isatidis selenium polysaccharide in feeding water pressed example 1 100ml/300L, example 2 100ml/300L, and example 3 100ml/300L, are respectively test group 1, test group 2, test group 3, and matched group is feeding not.。Feed 30 days, record chicken group's Gain weight.Table 6 is the anti-heat stress result of the test of Radix Isatidis selenium polysaccharide oral liquid to broiler.
Table 6: the anti-heat stress result of the test of Radix Isatidis selenium polysaccharide oral liquid to broiler
From above experimental result, draw, Radix Isatidis selenium polysaccharide oral liquid can significantly improve the autoimmunity of pig, broiler, increases resistance against diseases.
Claims (4)
1. a rich selenium Banlangen Polysaccharide oral liquid, is characterized in that: comprise the Radix Isatidis selenium polysaccharide of 1～20 part, sodium thiosulfate and the purified water of the former weight 0.2～0.5%.
2. a kind of rich selenium Banlangen Polysaccharide oral liquid according to claim 1, is characterized in that: the preparation method of described Radix Isatidis selenium polysaccharide comprises the following steps:
Prepare Banlangen Polysaccharide:
(1) Radix Isatidis and water are added in Low Temperature Thermal reflux, extract, tank by weight 1:5～15, temperature conditions is 70 degrees Celsius, and pressure is 0.25 MPa;
(2) the sodium chloride that adds Radix Isatidis and water gross weight 0.1～0.5%, then rises to 45～75 degrees Celsius by temperature in extraction pot and extracts, and extraction time is 2～8 hours
(3) collect extracting solution, under the condition of 0.3 MPa, carry out concentrating under reduced pressure, until relative density is 1.1～1.35 to stop;
(4) step product is (3) washed by 95% ethanol or absolute ether, centrifugal rear collection supernatant under per minute 500～1500 conditions that turn then, then supernatant is made to Banlangen Polysaccharide after by 120 degrees Celsius of heated-air circulation oven inner dryings;
Prepare Radix Isatidis selenium polysaccharide:
(1) Banlangen Polysaccharide and selenizing agent are added in reactor by the weight ratio of 1:0.2-0.85 jointly;
(2) reacting by heating still, makes temperature in it rise to 40～65 degrees Celsius, adds catalyst;
(3) centrifugation step product (2) under per minute 500～1500 conditions that turn, then adsorb with the macroporous resin of 100～500 nanometers;
(4) step macroporous resin is (3) washed with absolute ether, the product of eluting is through centrifugal under per minute 500～1500 conditions that turn, and gleanings makes Radix Isatidis selenium polysaccharide after by 120 degrees Celsius of heated-air circulation oven inner dryings.
3. a kind of rich selenium Banlangen Polysaccharide oral liquid according to claim 2, it is characterized in that: prepare Radix Isatidis selenium polysaccharide step (2) described catalyst be rare nitric acid or barium chloride, the weight ratio of catalyst and selenizing agent is 0.2～0.45:1, and the catalytic reaction time is 2～12 hours.
4. the preparation method of a kind of rich selenium Banlangen Polysaccharide oral liquid according to claim 1, is characterized in that: comprise the following steps:
(1) Radix Isatidis selenium polysaccharide is mixed with sodium thiosulfate, add in Agitation Tank;
(2) add again purified water, stir 15～30 minutes;
(3) by 0.45 micron of filtration for step product (2), after subpackage, make finished product.
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Cited By (1)
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|---|---|---|---|---|
| CN105330758A (en) * | 2015-11-16 | 2016-02-17 | 浙江省农业科学院 | Method for extracting polysaccharide from needle mushroom roots |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102000105A (en) * | 2010-10-11 | 2011-04-06 | 西北农林科技大学 | Compound timicosin oral liquid and preparation method thereof |
| CN102532339A (en) * | 2012-02-14 | 2012-07-04 | 兰州大学 | Method for selenizing pilose asiabell root polysaccharides and application of product |
-
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102000105A (en) * | 2010-10-11 | 2011-04-06 | 西北农林科技大学 | Compound timicosin oral liquid and preparation method thereof |
| CN102532339A (en) * | 2012-02-14 | 2012-07-04 | 兰州大学 | Method for selenizing pilose asiabell root polysaccharides and application of product |
Non-Patent Citations (1)
| Title |
|---|
| 盛家荣等: "均匀设计优选南板蓝根多糖的提取工艺", 《中药材》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105330758A (en) * | 2015-11-16 | 2016-02-17 | 浙江省农业科学院 | Method for extracting polysaccharide from needle mushroom roots |
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